Ventilation base plate capable of uniformly bubbling

Abstract

The utility model provides a ventilation base plate with uniform bubbling, which is arranged at the bottom of a cell culture bag and used for ventilating the cell culture bag, and comprises a ventilation base plate, the ventilation base plate comprises a base plate and ventilation sheets, one side of the base plate is provided with a plurality of placing grooves used for placing the ventilation sheets, and the ventilation sheets comprise a sintering part, a transition part and a ventilation part which are sequentially arranged in a step shape. The containing groove comprises a groove bottom communicated with the ventilation pipeline and a groove wall used for limiting the ventilation piece, the groove wall extends in the direction of the center axis of the containing groove to form a step, the step comprises a first table top and a second table top, the first table top is provided with a sintering ring cone, and one end of the transition part abuts against the second table top. According to the ventilation base plate with the uniform bubbling, the transition part is arranged on the ventilation sheet, and the transition part is used for preventing a molten sintering ring cone material from diffusing to the ventilation part and blocking the ventilation hole when the ventilation sheet is mounted, so that the ventilation hole is utilized to the maximum extent, and the ventilation efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedicine, and in particular to a ventilation chassis with uniform bubbling. Background Art

[0002] Cell culture is a method of simulating an in vivo environment (including sterility, a suitable temperature, pH, and nutritional conditions) in vitro, enabling cells to survive, grow, reproduce, and maintain their primary structure and function. During cell culture, ventilation at the top of the culture bag is insufficient to meet the cells' need for dissolved oxygen. Therefore, ventilation from the bottom of the reaction bag is commonly used.

[0003] However, the diameter of cells is generally 10-100 microns, and the volume of bubbles is similar to the size of cells. When bubbles burst in the culture medium, they can easily cause large shear force damage to the surrounding cells. For this reason, the volume of the bubbles needs to be reduced. In order to reduce the volume of the bubbles, the aperture of the bottom ventilation device needs to be reduced accordingly; at the same ventilation flow rate, the bubbles coming out of the smaller aperture will be relatively smaller. Not only can the damage to cells when the bubbles burst be reduced, but also, the surface area of ​​the bubbles coming out at the same ventilation volume will be larger. Correspondingly, the larger the contact area between the gas and the culture medium, the faster the interaction between the gas and the culture medium will be, resulting in a better effect of gas introduction.

[0004] In the prior art, small-pore bubbles are achieved by squeezing gas through a sintered sheet with fine pores. The sintered sheet needs to form a sealed connection with the base, specifically by melting part of the material on the base, allowing the molten material to enter the fine pores of the sintered sheet. After the material cools and takes shape, the sintered sheet and the base can be connected as one. However, after melting, the material will pass upward through the fine pores of the sintered sheet until it reaches the upper surface of the sintered sheet, forming an airtight surface condensation layer on the upper surface that is wider than the material after condensation inside the sintered sheet. Although the bubbles that emerge directly through the sintered sheet meet the requirements of small pores, some small-volume bubbles will accumulate under the upper airtight condensation layer. When the accumulated gas volume reaches a critical value, they will all emerge from the boundary of the condensation layer to form a large bubble. This large bubble can cause cell damage and reduce ventilation efficiency. Utility Model Content

[0005] In response to the shortcomings of the existing technology, the purpose of the present invention is to provide a ventilation chassis with uniform bubbling, which aims to solve the technical problem in the existing technology that after melting, the material will pass through the pores of the sintered sheet upward to the upper surface of the sintered sheet, forming an airtight surface condensation layer on the upper surface that is wider than the material after condensation inside the sintered sheet.

[0006] In order to achieve the above-mentioned purpose, the present invention is implemented through the following technical solutions: a ventilation chassis with uniform bubbling, installed at the bottom of a cell culture bag for ventilating the cell culture bag, comprising a ventilation chassis, the ventilation chassis comprising a chassis and a ventilation sheet, a plurality of placement grooves for placing the ventilation sheets are provided on one side of the chassis, the placement grooves comprising a groove bottom and a groove wall for limiting the position of the ventilation sheet, the ventilation sheet comprising a ventilation portion and a sintering portion for airtight connection with the placement groove, the ventilation sheet also comprising a transition portion connected between the sintering portion and the ventilation portion, the ventilation sheet is arranged in a step shape, the ventilation portion is provided with a ventilation hole, the groove wall extends toward the central axis direction of the placement groove to form a step, the step comprises a first table top and a second table top, the first table top is provided with a sintering ring cone for fixing the sintering portion, and one end of the transition portion is abutted against the second table top.

[0007] Compared with the prior art, the beneficial effect of the present invention lies in: adopting the above-mentioned design scheme, after the sintered ring cone is dissolved, the sintered part is fixed on the first table surface, and cannot extend to the ventilation part due to the obstruction of the transition part, so that no condensation layer is generated, thereby ensuring the integrity of the ventilation part, maximizing the ventilation area of ​​the ventilation part, and not causing large bubbles to be generated during ventilation due to the generated condensation layer, which will generate large shear force on the cells.

[0008] According to one aspect of the above technical solution, a ventilation duct communicating with the bottom of the trough is provided on one side of the chassis close to the bottom of the trough.

[0009] According to one aspect of the above technical solution, the second table surface is spaced apart from the bottom of the groove. This design can ensure that the bubble outlet area of ​​the vent sheet is maximized.

[0010] According to one aspect of the above technical solution, the sintering ring cone is arranged in the middle of the first table top, and the cross section of the sintering ring cone is arranged in a triangular shape. By setting the cross section of the sintering ring cone into a triangle, it can be evenly diffused around the sintering part after dissolution, so that the sintering part and the first table top are fixed more firmly and the airtightness is better.

[0011] According to one aspect of the above technical solution, an anti-overflow ring groove is provided on the second table surface away from the center of the placement groove. With this design, after the sintering ring cone material is dissolved, the overflowed sintering ring cone and the sintering table surface material in a molten state are received to prevent overflow and blockage of the vent holes on the vent plate. At the same time, the anti-overflow ring groove facilitates assembly and avoids assembly difficulties caused by the mismatch between the rounded corners at the bottom of the vent plate and the step corner angles of the placement groove.

[0012] According to one aspect of the above technical solution, a stirring paddle mounting shaft is provided on one side of the chassis close to the placement tank, and the stirring paddle is a magnetically coupled driven stirring paddle. The stirring paddle driven by magnetic coupling can stir the nutrient solution inside the reaction bag without contacting the internal environment.

[0013] According to one aspect of the above technical solution, the edge of the chassis is provided with a welding edge. By providing the welding edge on the edge of the chassis, it is convenient to weld the chassis and the culture bag together, so that an independent space is formed inside the culture bag. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of a ventilated chassis structure with uniform bubbling and a reaction bag in one embodiment of the present invention;

[0015] Figure 2 This is a schematic diagram of a ventilation chassis structure with uniform bubbling in one embodiment of the present utility model;

[0016] Figure 3 This is a partial cross-sectional view of a ventilated bottom plate with uniform bubbling in one embodiment of the present invention;

[0017] Explanation of the main component symbols: 1- chassis, 11- placement groove, 111- groove wall, 112- groove bottom, 113- first table top, 114- second table top, 115- overflow ring groove, 12- sintering ring cone, 13- ventilation pipe, 14- stirring paddle mounting shaft, 15- welding edge, 2- ventilation sheet, 21- ventilation part, 211- vent hole, 22- transition part, 23- sintering part, 3- stirring paddle, 4- reaction bag;

[0018] The following specific embodiments will further illustrate the present invention in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0019] To facilitate understanding of the present invention, a more comprehensive description of the present invention will be provided below with reference to the accompanying drawings. The drawings illustrate several embodiments of the present invention. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and comprehensive understanding of the present invention.

[0020] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be an intermediate element. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only.

[0021] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used herein in the specification of this invention are intended only to describe specific embodiments and are not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0022] See also Figures 1 to 2 , an embodiment of the present invention provides a ventilation base with uniform bubbling, which is installed at the bottom of the cell culture bag for ventilating the cell culture bag, including a ventilation base, the ventilation base includes a base 1 and a ventilation sheet 2, and one side of the base 1 is provided with a plurality of placement grooves 11 for placing the ventilation sheets 2, wherein the side of the base 1 facing away from the placement grooves 11 is provided with a ventilation pipe 13 connected to the placement grooves 11, the ventilation sheet 2 includes a ventilation portion 21, and a sintering portion 23 for airtight connection with the placement grooves 11, the ventilation sheet 2 also includes a transition portion 22 connected between the sintering portion 23 and the ventilation portion 21, the ventilation sheet 2 is arranged in a step-shaped manner, the ventilation portion 21 is provided with ventilation holes 211, the placement groove 11 includes a groove bottom 112 connected to the ventilation pipe 13, and a groove wall 111 for limiting the ventilation sheet 2, wherein the diameter of the ventilation pipe 13 is smaller than the diameter of the groove bottom 112 of the placement groove 11; the groove wall 111 is directed to The placement groove 11 extends in the direction of the central axis to form a step, and the step includes a first table surface 113 and a second table surface 114. The first table surface 113 is provided with a sintering ring cone 12 for fixing the sintering portion 23. The placement groove 11 and the chassis 1 away from the ventilation pipe 13 form a confluence channel through the ventilation hole 211. In this embodiment, the side of the chassis 1 facing the interior of the reaction bag 4 is also provided with a stirring paddle mounting shaft 14 and a stirring paddle 3, and the internal nutrient solution is stirred by the stirring paddle 3 so that the internal nutrient solution is evenly mixed; specifically, the chassis 1 is provided with a stirring paddle mounting shaft 14 on the side close to the placement groove 11, and the stirring paddle 3 is a magnetic coupling driven stirring paddle 3; it should be noted that in this embodiment, the stirring paddle 3 is driven by magnetic coupling, so that the stirring paddle 3 can be driven to rotate without contacting the stirring paddle 3, which can avoid contamination of the interior of the reaction bag 4 and leakage of the reaction bag 4, and the structure is simple.

[0023] See also Figure 3In this embodiment, the sintered cone 12 disposed on the first table 113 is triangular in shape. When the vent sheet 2 is placed on the placement groove 11, the sintered portion 23 contacts the triangular top of the sintered cone 12. During assembly and heating, the triangular top of the sintered cone 12 melts first. Under the action of gravity, the sintered portion 23 moves downward, and the melted sintered cone 12 material flows down along the sides of the triangle, thus being evenly distributed on the first table 113. After the sintered portion 23 and the sintered cone 12 are compatible and solidified, the sintered portion 23 is connected to the first table 113. The sintered cone 12 material fills the gap between the sintered portion 23 and the first table 113, forming a single body with the vent sheet 2, thereby forming an airtight connection with the first table 113. Specifically, the sintered cone 12 is disposed in the middle of the first table 113 and has a triangular cross-section. However, when the sintered ring cone 12 melts, it will inevitably diffuse to the surroundings along the ventilation plate 2. In this embodiment, in order to prevent the melted sintered ring cone 12 material from diffusing to the ventilation portion 21 of the ventilation plate 2, the ventilation holes 211 of the ventilation portion 21 are blocked and the ventilation area of ​​the ventilation portion 21 is reduced. For this purpose, a transition portion 22 is provided between the ventilation portion 21 and the sintered portion 23, and the transition portion 22 is used to prevent the melted sintered ring cone 12 material from diffusing to the ventilation portion 21.

[0024] See also Figure 3 In this embodiment, since the vent sheet 2 is arranged in a stepped shape, after the vent sheet 2 is placed in the placement groove 11, the sintered portion 23 of the vent sheet 2 fits against the first table 113, and one end of the transition portion 22 abuts against the second table 114; it can be understood that the overflowed sintered ring cone 12 will flow into the second table 114 along the gap between the transition portion 22 and the step side wall. In order to avoid the melted sintered ring cone 12 flowing along the second table 114 to the vent portion 21, affecting the ventilation area of ​​the vent portion 21, an anti-overflow ring groove 115 is provided on the side of the second table 114 away from the center of the placement groove 11, and the overflowed sintered ring cone 12 material flows directly into the anti-overflow ring groove 115 along the groove wall 111, thereby preventing the sintered ring cone 12 material from flowing into the vent portion 21 along the transition portion 22; preferably, the second table 114 is provided with an anti-overflow ring groove 115 on the side away from the center of the placement groove 11. It should be noted that the anti-overflow ring groove 115 is also convenient for assembly, avoiding assembly difficulties caused by the mismatch between the bottom radius of the vent plate 2 and the step corner angle of the placement groove 11, and the sintered ring cone 12 material flowing into the anti-overflow ring groove 115 merges with the transition part 22 after solidification, further strengthening the fixation of the vent plate 2 and the placement groove 11.

[0025] See also Figure 3In this embodiment, the diameter of the ventilation pipe 13 is smaller than the diameter of the bottom 112 of the placement groove 11. In order to avoid external ventilation, the gas directly passes through the ventilation pipe 13 to ventilate the ventilation part 21 of the ventilation sheet 2; further, the second table 114 is spaced apart from the bottom 112 of the groove. With this design, the ventilation area of ​​the vent hole 211 can be fully utilized, and a gas accommodating cavity is formed between the ventilation sheet 2 and the bottom 112 of the groove, thereby playing a buffering role.

[0026] Furthermore, a welding edge 15 is provided on the edge of the chassis 1 , and an airtight connection is achieved with the reaction bag 4 through the welding edge 15 .

[0027] It should be noted that, in this embodiment, the inner diameters of the multiple placement slots 11 arranged on the chassis 1 are all the same. It can be understood that the outer diameters of the corresponding ventilation plates 2 are the same, but the apertures of the ventilation holes 211 of the ventilation parts 21 of the ventilation plates 2 installed inside the respective placement slots 11 are different and can be replaced as needed to suit different experimental scenarios.

[0028] In summary, the ventilation chassis with uniform bubbling in the above embodiments of the present invention prevents the sintered ring cone from flowing into the ventilation portion in a melted state by setting a transition portion on the ventilation sheet, thereby maximizing the utilization of the ventilation holes of the ventilation sheet and improving the ventilation efficiency; and an anti-overflow ring groove is set on the second table surface. The anti-overflow ring groove not only accommodates the excess melted sintered ring cone, but also facilitates the installation of the ventilation sheet and the placement groove. In addition, the sintered ring cone material flowing into the anti-overflow ring groove is further strengthened by bonding with the transition portion to fix the ventilation sheet and the prevention groove.

[0029] Throughout this specification, reference to terms such as "one embodiment," "some embodiments," "examples," "specific examples," or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0030] The above-described embodiments merely represent several implementations of the present invention. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make various modifications and improvements without departing from the spirit of the present invention, and these modifications and improvements fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. A ventilation base with uniform bubbling, installed at the bottom of a cell culture bag for ventilating the cell culture bag, comprising a ventilation base, characterized in that: The ventilation chassis includes a chassis and a ventilation sheet. A plurality of placement grooves for placing the ventilation sheets are provided on one side of the chassis. The placement grooves include a groove bottom and a groove wall for limiting the position of the ventilation sheet. The ventilation sheet includes a ventilation portion and a sintering portion for airtight connection with the placement groove. The ventilation sheet also includes a transition portion connected between the sintering portion and the ventilation portion. The ventilation sheet is arranged in a stepped shape. The ventilation portion is provided with ventilation holes. The groove wall extends toward the central axis of the placement groove to form a step. The step includes a first table top and a second table top. The first table top is provided with a sintering ring cone for fixing the sintering portion. One end of the transition portion abuts against the second table top.

2. The ventilated bottom plate with uniform bubbling according to claim 1, characterized in that: A ventilation pipe communicating with the bottom of the trough is provided on one side of the chassis close to the bottom of the trough.

3. The ventilated bottom plate with uniform bubbling according to claim 2, characterized in that: The second table surface is spaced apart from the groove bottom.

4. The ventilated bottom plate with uniform bubbling according to claim 3, characterized in that: The sintered ring cone is arranged in the middle of the first table surface, and the cross section of the sintered ring cone is triangular.

5. The ventilated bottom plate with uniform bubbling according to claim 4, characterized in that: The second table surface is provided with an anti-overflow ring groove on a side away from the center of the placement groove.

6. The ventilated bottom plate with uniform bubbling according to claim 2, characterized in that: A stirring paddle installation shaft is provided on one side of the chassis close to the placement tank, and the stirring paddle is a magnetic coupling driven stirring paddle.

7. The ventilated bottom plate with uniform bubbling according to claim 2, characterized in that: The edge of the chassis is provided with a welding edge.

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