Plasmid construction device

Abstract

The utility model discloses a plasmid construction device, and belongs to the technical field of bioengineering. Comprising a monitoring module, a pipetting module, a microplate reader, a liquid storage module, an elisa plate, a purification module, an electrophoresis module, a gel cutting module and a PCR instrument, the monitoring module is electrically connected with the pipetting module, the microplate reader, the purification module, the electrophoresis module and the gel cutting module, and the pipetting module can be in butt joint with the purification module, the liquid storage module and the elisa plate; the electrophoresis module is used for electrophoresis, and the gel cutting module is used for cutting gel of a gelatinous structure; the purification module is used for purifying the gelatinous target sample; the liquid transfer module can transfer a sample solvent and water in the liquid storage module to the elisa plate, the elisa plate can be placed in an elisa reader for concentration detection, the monitoring module enables the liquid transfer module to transfer the liquid storage module to the elisa plate according to preset information and detection information of the elisa reader, so that the sample solvent of the elisa plate reaches a preset concentration, and the PCR reader is used for plasmid construction. And the experiment efficiency and accuracy are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of bioengineering, in particular to a plasmid construction device. Background Art

[0002] Plasmid construction is a fundamental task in molecular biology research, including genetic engineering and cell biology. The traditional plasmid construction process involves multiple steps, including PCR cloning of the target gene fragment, electrophoretic separation, cleavage and recovery, product purification, and plasmid construction. These steps typically require separate, manual operations using different instruments and equipment, making them tedious and error-prone. Therefore, a plasmid construction device is urgently needed to address these challenges. Utility Model Content

[0003] The purpose of the utility model is to provide a plasmid construction device to realize plasmid construction, improve experimental efficiency, avoid manual operation, reduce human errors, and improve experimental accuracy.

[0004] To achieve this purpose, the present invention adopts the following technical solutions:

[0005] A plasmid construction device, comprising a monitoring module, a pipetting module, a microplate reader, a liquid storage module, a microplate plate, a purification module, an electrophoresis module, a PCR plate, a gel cutting module, and a PCR instrument, wherein the monitoring module is electrically connected to the pipetting module, the PCR instrument, the microplate reader, the purification module, the electrophoresis module, and the gel cutting module, respectively; and the pipetting module is capable of docking with the purification module, the liquid storage module, the PCR plate, and the microplate plate, respectively.

[0006] The electrophoresis module is used to perform electrophoresis on the PCR sample to form a gel-like structure, and the gel cutting module is used to cut the gel-like structure to obtain a gel-like target sample;

[0007] The purification module is used to purify the gel-like target sample to form a purified sample solvent;

[0008] The pipetting module can transfer the sample solvent and the water in the liquid storage module to the ELISA plate, and the ELISA plate can be placed in the ELISA instrument for concentration detection. The monitoring module enables the pipetting module to transfer the water in the liquid storage module to the ELISA plate based on preset information and the detection information of the ELISA instrument, so that the sample solvent of the ELISA plate reaches a preset concentration. The pipetting module can transfer the plasmid construction reaction solution in the liquid storage module and the sample solvent in the ELISA plate to the PCR plate, and the PCR instrument is used to run the plasmid construction reaction program.

[0009] In some possible embodiments, the purification module includes an oscillation module, a filtration module, and a centrifugation module, all electrically connected to the monitoring module. The oscillation module, the filtration module, and the centrifugation module can purify the gel-like target sample to obtain a purified sample solvent.

[0010] In some possible embodiments, a frame is further included, and the pipetting module, liquid storage module, purification module, and electrophoresis module are all arranged on the frame. The gel cutting module includes a visual recognition component, a motion component, and a gel cutting head connected to the motion component. The visual recognition component is arranged on the frame, and the motion component and the visual recognition component are both electrically connected to the monitoring module.

[0011] In some possible implementations, a film sealing device is further included, wherein the film sealing device is electrically connected to the monitoring module and is capable of sealing the PCR plate.

[0012] In some possible embodiments, the pipetting module further includes an X-axis moving module, a Y-axis moving module connected to the moving end of the X-axis moving module, and a first Z-axis moving module and a second Z-axis moving module both connected to the moving end of the Y-axis moving module, the moving end of the first Z-axis moving module is connected to a first pipetting component, the moving end of the second Z-axis moving module is connected to a second pipetting component, and the first pipetting component and the second pipetting component have different measuring ranges.

[0013] In some possible embodiments, the first pipette assembly includes a first power pump and a first pipette connected to the first power pump, and the first pipette is arranged at the moving end of the first Z-direction moving module; the second pipette assembly includes a second power pump and a second pipette connected to the second power pump, and the second pipette is arranged at the moving end of the second Z-direction moving module; the first pipette and the second pipette have different measuring ranges.

[0014] In some possible embodiments, a pipette box is further included, wherein the pipette box includes a first pipette and a second pipette, and the pipetting module can be docked with the pipette box to replace the first pipette or the second pipette.

[0015] In some possible embodiments, it further includes a consumables stack, a recycling station and a transport module. The unused pipette box is arranged on the consumables stack. The transport module can transfer the pipette box from the consumables stack to a preset position and can transfer the used pipette box to the recycling station.

[0016] In some possible implementations, the transport module includes a third Z-direction moving module and a clamp connected to a moving end of the third Z-direction moving module, and the third Z-direction moving module is connected to the moving end of the Y-direction moving module.

[0017] In some possible embodiments, the apparatus further includes a frame and a monitor disposed on the frame, wherein the pipetting module, the liquid storage module, the ELISA plate, the purification module, the electrophoresis module and the gel cutting module are all disposed on the frame, and the monitor is used to monitor the plasmid construction device.

[0018] Beneficial effects of the utility model:

[0019] The utility model provides a plasmid construction device, which automatically realizes the experimental operations of electrophoretic separation, cutting, purification, uniform concentration of sample solvent and plasmid construction through functional modules such as a monitoring module and a pipetting module, a purification module, an enzyme-labeled instrument, an electrophoresis module and a gel cutting module, that is, by integrating multiple functional modules and realizing automated operation, the experimental efficiency is improved. Human error is reduced, the operating steps are accurately controlled, and the sample concentration is quantitatively analyzed using a full-wavelength enzyme-labeled instrument to ensure the accuracy and repeatability of the experimental results. In addition, through modular design, each module can be flexibly configured, that is, each functional module such as a pipetting module, an electrophoresis module and a gel cutting module, a purification module, an enzyme-labeled instrument, etc. can be freely matched to meet the needs of different experimental scenarios, thereby improving the flexibility and scalability of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of a plasmid construction device provided in a specific embodiment of the present utility model;

[0021] Figure 2 yes Figure 1 Enlarged view of point A.

[0022] In the picture:

[0023] 1. Frame; 2. Pipetting module; 21. Y-axis moving module; 22. X-axis moving module; 23. First Z-axis moving module; 24. Second Z-axis moving module; 25. First pipetting assembly; 251. First power pump; 252. First pipette; 26. Second pipetting assembly; 261. Second power pump; 262. Second pipette; 27. Third pipetting assembly; 28. Fourth pipetting assembly; 3. Electrophoresis module; 4. Gel cutting module ; 41. Visual recognition component; 42. Motion component; 43. Glue cutting head; 5. Oscillation module; 6. Filtration module; 7. Centrifugation module; 8. PCR instrument; 9. Film sealing instrument; 10. Pipette box; 11. Consumables stack; 12. Recycling station; 13. Transport module; 131. Third Z-axis moving module; 132. Gripper; 14. Monitor; 15. Air purification system; 16. Consumables extraction mechanism; 17. Electrophoresis buffer container. DETAILED DESCRIPTION

[0024] To make the technical problems solved by the present invention, the technical solutions adopted, and the technical effects achieved more clearly, the technical solutions of the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work shall fall within the scope of protection of the present invention.

[0025] In the description of this utility model, unless otherwise specified or limited, the terms "connected," "connect," and "fixed" should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0026] In the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Moreover, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0027] like Figure 1 and Figure 2As shown, this embodiment provides a plasmid construction device, including a monitoring module, a pipetting module 2, an ELISA reader, a liquid storage module, an ELISA plate, a purification module, a PCR plate, a PCR instrument, an electrophoresis module 3, and a gel cutting module 4. The monitoring module is electrically connected to the pipetting module 2, the ELISA reader, the purification module, the PCR instrument 8, the electrophoresis module 3, and the gel cutting module 4, respectively. The pipetting module 2 can be docked with the purification module, the liquid storage module, the PCR plate, and the ELISA plate. The electrophoresis module 3 is used to perform electrophoresis on the PCR sample to form a gel-like structure, and the gel cutting module 4 is used to cut the gel-like structure to obtain a gel-like target sample. The purification module is used to purify the gel-like target sample to form a purified sample solvent. The pipetting module 2 can transfer both the sample solvent and the water in the liquid storage module to the ELISA plate, which can be placed in the ELISA reader for concentration detection. The monitoring module, based on preset information and the detection information of the ELISA reader, causes the pipetting module 2 to transfer the water in the liquid storage module to the ELISA plate so that the sample solvent in the ELISA plate reaches a preset concentration. The liquid transfer module 2 can transfer the plasmid construction reaction solution in the liquid storage module and the sample solvent in the ELISA plate to the PCR plate. The PCR instrument 8 is used to run the plasmid construction reaction program.

[0028] Through the monitoring module and the functional modules such as the pipetting module 2, the purification module, the microplate reader, the electrophoresis module 3, the gel cutting module 4 and the PCR instrument 8, the experimental operations of electrophoretic separation, cutting, purification, uniform concentration of sample solvent and plasmid construction are automatically realized, that is, by integrating multiple functional modules and realizing automated operation, the experimental efficiency is improved. Human error is reduced, the operation steps are accurately controlled, and the sample concentration is quantitatively analyzed using a full-wavelength microplate reader to ensure the accuracy and repeatability of the experimental results. In addition, through modular design, each module can be flexibly configured, that is, each functional module such as the pipetting module 2, the electrophoresis module 3 and the gel cutting module 4, the purification module, the microplate reader and the PCR instrument 8 can be freely matched to meet the needs of different experimental scenarios, thereby improving the flexibility and scalability of the device.

[0029] The plasmid construction device also includes a frame 1 and a monitor 14 disposed on the frame 1. The pipetting module 2, microplate reader, liquid storage module, microplate plate, purification module, PCR instrument 8, electrophoresis module 3, and gel cutting module 4 can all be disposed on the frame 1, or can be partially disposed on the frame 1 as needed. The user can monitor the plasmid construction device, that is, monitor the aforementioned modules, through the monitor 14 to understand the experimental steps in real time. Specifically, the monitor 14 can provide panoramic monitoring.

[0030] Optionally, the plasmid construction device also includes a sealer 9, electrically connected to the monitoring module. The sealer 9 can seal the PCR plate, which holds the reaction reagents, ensuring the seal and sterility of the PCR sample during the amplification process. Furthermore, the PCR plate can be placed in a PCR instrument 8 for amplification to form a PCR sample, enabling PCR sample amplification and facilitating subsequent electrophoresis and other operations, further enabling integrated operation of the device.

[0031] The gel cutting module 4 includes a visual recognition component 41, a motion component 42 and a gel cutting head 43 connected to the motion component 42. The visual recognition component 41 is arranged on the frame 1, and the motion component 42 and the visual recognition component 41 are both electrically connected to the monitoring module. After the electrophoresis module 3 runs the gel, the visual recognition component 41 is used, such as a camera, to take pictures and images. The monitoring module automatically calculates, selects a specific segment, and calculates the gel cutting position. The motion component 42 drives the gel cutting head 43 to automatically cut the selected strip, that is, the gel-like target sample. Optionally, the motion component 42 can be a manipulator or a linear module or a combination of a motor and a lead screw nut in the prior art, etc., which can move in a horizontal plane or move in one direction, and can be set according to specific needs.

[0032] The purification module includes an oscillation module 5, a filtration module 6, and a centrifugation module 7, all electrically connected to the monitoring module. These modules are capable of purifying the gel-like target sample to obtain a purified sample solvent. During use, the gel-like target sample is first dissolved by the oscillation module 5, followed by preliminary purification by the filtration module 6. The purified sample is then further purified and collected using the centrifugation module 7 to ensure that the resulting sample meets downstream requirements. Exemplarily, the oscillation module 5 is a constant-temperature oscillator that heats the gel-like target sample and oscillates it at a constant speed, accelerating its dissolution. The centrifugal module 7 is a centrifuge, and the filtration module 6 is a negative pressure filtration structure. For example, the negative pressure filtration structure includes a container, on which a filtration plate is installed. The filtration plate and the container form an internal space. The container is connected to a vacuum pump to evacuate the internal space, so that impurities and washing liquid in the filtration plate are discharged into a waste liquid bottle connected to an empty box. The filtration plate is transferred to a recovery plate, and after adding water or eluent, it is placed on a centrifuge for centrifugation to elute the purified product and collect it in the recovery plate. The above structures are all prior art, and the specific structure and method of use are all referred to the prior art and will not be repeated here.

[0033] The pipetting module 2 also includes an X-axis moving module 22, a Y-axis moving module 21 connected to the moving end of the X-axis moving module 22, and a first Z-axis moving module 23 and a second Z-axis moving module 24 both connected to the moving end of the Y-axis moving module 21. The moving end of the first Z-axis moving module 23 is connected to a first pipetting component 25, and the moving end of the second Z-axis moving module 24 is connected to a second pipetting component 26. The first pipetting component 25 and the second pipetting component 26 have different measuring ranges. The X-direction moving module 22 can drive the Y-direction moving module 21, the first Z-direction moving module 23 and the second Z-direction moving module 24, the first pipetting assembly 25 and the second pipetting assembly 26 to move along the X-direction, and the Y-direction moving module 21 can drive the first Z-direction moving module 23 and the second Z-direction moving module 24, the first pipetting assembly 25 and the second pipetting assembly 26 to move along the Y-direction, thereby enabling the first Z-direction moving module 23 and the second Z-direction moving module 24, the first pipetting assembly 25 and the second pipetting assembly 26 to move within the XY plane, thereby increasing the range of motion. The first Z-direction moving module 23 drives the first pipetting assembly 25 to move along the Z-direction, and the second Z-direction moving module 24 drives the second pipetting assembly 26 to move along the Z-direction. By setting the first Z-direction moving module 23 and the second Z-direction moving module 24, the first pipetting assembly 25 and the second pipetting assembly 26 can work independently. In this embodiment, the X-direction, Y-direction and Z-direction are perpendicular to each other.

[0034] Specifically, by using a full-wavelength microplate reader to quantitatively analyze the sample concentration, the monitoring module automatically uniformizes the reagent volume required for the concentration based on the sample concentration data. The pipetting module 2 uses the first pipetting component 25 or the second pipetting component 26 of different ranges to transfer different liquid volumes according to the reagent volume to achieve uniform sample concentration.

[0035] Optionally, a fifth Z-direction moving module and a third pipetting assembly 27 connected to the moving end of the fifth Z-direction moving module may be provided, and the fifth Z-direction moving module is connected to the moving end of the Y-direction moving module 21. The fifth Z-direction moving module and the third pipetting assembly 27 are movable in the XY plane, and the fifth Z-direction moving module is capable of driving the third pipetting assembly 27 to move in the Z direction.

[0036] Optionally, a fourth Z-direction moving module and a fourth pipetting assembly 28 connected to the moving end of the fourth Z-direction moving module can also be provided. The fourth Z-direction moving module is connected to the moving end of the motion assembly 42 to avoid excessive load on the X-direction moving module 22 and the Y-direction moving module 21, or structural interference between multiple pipetting assemblies, etc., to facilitate structural layout. The fourth Z-direction moving module and the fourth pipetting assembly 28 can move in the XY plane through the motion assembly 42. The fourth Z-direction moving module enables the fourth pipetting assembly 28 to move along the Z direction, thereby working independently with the rubber cutting head 43. The four pipetting assemblies have different measuring ranges. By increasing the number of pipetting assemblies, different liquid volumes can be further accurately transferred to achieve uniform sample concentration.

[0037] The first pipetting assembly 25 includes a first power pump 251 and a first pipette 252 connected to the first power pump 251. The first pipette 252 is located at the movable end of the first Z-axis movable module 23. Specifically, the first power pump 251 can be a micropump connected to the movable end of the first Z-axis movable module 23. The first pipette 252 and the micropump are directly connected, resulting in a simple and compact structure. Similarly, the second pipetting assembly 26 includes a second power pump 261 and a second pipette 262 connected to the second power pump 261. The second pipette 262 is located at the movable end of the second Z-axis movable module 24. The first pipette 252 and the second pipette 262 have different measuring ranges.

[0038] The plasmid construction device also includes a pipette box 10, which includes a first pipette 252 and a second pipette 262. The pipetting module 2 can be docked with the pipette box 10 to replace the first pipette 252 or the second pipette 262. By replacing the first pipette 252 and the second pipette 262, different reagents can be taken in and out multiple times to prevent cross contamination of reagents and affect the experimental results.

[0039] The plasmid construction device also includes a consumable material stack 11, a recycling station 12 and a handling module 13. The pipette box 10 is located at the consumable material stack 11. The handling module 13 can transfer the pipette box 10 from the consumable material stack 11 to a preset position, and can transfer the used pipette box 10 to the recycling station 12. A large amount of pipette boxes 10 can be stored by the consumable material stack 11, that is, a large amount of pipettes are stored, and the stock is sufficient to carry out multiple experiments. Specifically, the consumable material stack 11 can be a carrier, which is provided with a plurality of accommodating grooves for accommodating the pipette boxes 10. The handling module 13 can be a manipulator, which takes out the pipette box 10 from the accommodating groove and places it in the preset position. The recycling station 12 can be a casing, which has a larger opening, so that the handling module 13 transfers the used pipette box 10 into the casing.

[0040] In this embodiment, a consumable material extraction mechanism 16 is further included. The consumable material extraction mechanism 16 is used to remove the pipette box 10 from the consumable material stack 11. The transport module 13 and the consumable material extraction mechanism 16 are docked and placed in a preset position. The consumable material extraction structure can be a four-axis robot, etc., and can be implemented using existing technology, which will not be described in detail.

[0041] The transport module 13 includes a third Z-direction moving module 131 and a clamping claw 132 connected to the moving end of the third Z-direction moving module 131 . The third Z-direction moving module 131 is connected to the moving end of the Y-direction moving module 21 , simplifying the structure and reducing costs.

[0042] Optionally, the frame 1 is further provided with a plurality of containers for carrying different reagents, such as an electrophoresis buffer container 17, which can be automatically discharged into the electrophoresis tank before electrophoresis and automatically discharged back after electrophoresis without manual intervention. For example, the water container, the pipetting module 2 transfers the water in the water container to the ELISA plate so that the sample solvent of the ELISA plate reaches a preset concentration. This embodiment uses a monitor 14 for panoramic monitoring to ensure that containers carrying different reagents and other materials such as the pipette box 10 are placed in predetermined positions. The monitoring module automatically calculates the consumption and demand of consumables, and performs transportation and stack management through the handling module 13.

[0043] Optionally, an air purification system 15 is also installed on the frame 1 to purify the air on the device to ensure the accuracy of the experiment and prevent pollution. The specific air purification system 15 can adopt an air purifier in the prior art, etc., which will not be described in detail.

[0044] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention, and are not intended to limit the implementation methods of the present invention. Those skilled in the art will appreciate that other variations or modifications can be made based on the above description. It is not necessary and impossible to enumerate all implementation methods here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the claims of the present invention.

Claims

1. A plasmid construction device, characterized in that: The invention comprises a monitoring module, a liquid transfer module (2), an enzyme-labeled instrument, a liquid storage module, an enzyme-labeled plate, a purification module, an electrophoresis module (3), a gel cutting module (4), a PCR plate and a PCR instrument (8), wherein the monitoring module is electrically connected to the liquid transfer module (2), the enzyme-labeled instrument, the purification module, the electrophoresis module (3), the PCR instrument (8) and the gel cutting module (4), respectively, and the liquid transfer module (2) can be docked with the purification module, the liquid storage module, the PCR plate and the enzyme-labeled plate respectively; The electrophoresis module (3) is used to perform electrophoresis on the PCR sample to form a gel-like structure, and the gel cutting module (4) is used to cut the gel-like structure to obtain a gel-like target sample; The purification module is used to purify the gel-like target sample to form a purified sample solvent; The pipetting module (2) can transfer the sample solvent and the water in the liquid storage module to the ELISA plate, and the ELISA plate can be placed in the ELISA instrument for concentration detection. The monitoring module causes the pipetting module (2) to transfer the water in the liquid storage module to the ELISA plate according to preset information and detection information of the ELISA instrument, so that the sample solvent in the ELISA plate reaches a preset concentration; The liquid transfer module (2) is capable of transferring the plasmid construction reaction solution in the liquid storage module and the sample solvent in the ELISA plate to the PCR plate, and the PCR instrument (8) is used to run the plasmid construction reaction program.

2. The plasmid construction device according to claim 1, characterized in that The purification module comprises an oscillation module (5), a filtration module (6) and a centrifugal module (7) all electrically connected to the monitoring module. The oscillation module (5), the filtration module (6) and the centrifugal module (7) are capable of purifying a gel-like target sample to obtain a purified sample solvent.

3. The plasmid construction device according to claim 1, characterized in that The invention also includes a frame (1), wherein the pipetting module (2), the liquid storage module, the purification module, and the electrophoresis module (3) are all arranged on the frame (1), and the gel cutting module (4) includes a visual recognition component (41), a motion component (42), and a gel cutting head (43) connected to the motion component (42), wherein the visual recognition component (41) is arranged on the frame (1), and the motion component (42) and the visual recognition component (41) are both electrically connected to the monitoring module.

4. The plasmid construction device according to claim 1, characterized in that It also includes a film sealing device (9), which is electrically connected to the monitoring module and can seal the PCR plate.

5. The plasmid construction device according to claim 1, characterized in that The pipetting module (2) further comprises an X-direction moving module (22), a Y-direction moving module (21) connected to the moving end of the X-direction moving module (22), and a first Z-direction moving module (23) and a second Z-direction moving module (24) both connected to the moving end of the Y-direction moving module (21), wherein the moving end of the first Z-direction moving module (23) is connected to a first pipetting component (25), and the moving end of the second Z-direction moving module (24) is connected to a second pipetting component (26), and the first pipetting component (25) and the second pipetting component (26) have different measuring ranges.

6. The plasmid construction device according to claim 5, characterized in that The first pipetting assembly (25) includes a first power pump (251) and a first pipette (252) connected to the first power pump (251), and the first pipette (252) is arranged at the moving end of the first Z-direction moving module (23); the second pipetting assembly (26) includes a second power pump (261) and a second pipette (262) connected to the second power pump (261), and the second pipette (262) is arranged at the moving end of the second Z-direction moving module (24); the first pipette (252) and the second pipette (262) have different measuring ranges.

7. The plasmid construction device according to claim 6, characterized in that It also includes a pipette box (10), which includes a first pipette (252) and a second pipette (262). The pipetting module (2) can be docked with the pipette box (10) to replace the first pipette (252) or the second pipette (262).

8. The plasmid construction device according to claim 7, characterized in that The invention also includes a consumables stack (11), a recycling station (12) and a transport module (13). The unused pipette box (10) is arranged on the consumables stack (11). The transport module (13) can transfer the pipette box (10) from the consumables stack (11) to a preset position and can transfer the used pipette box (10) to the recycling station (12).

9. The plasmid construction device according to claim 8, characterized in that The transport module (13) includes a third Z-direction moving module (131) and a clamping claw (132) connected to the moving end of the third Z-direction moving module (131), and the third Z-direction moving module (131) is connected to the moving end of the Y-direction moving module (21).

10. The plasmid construction device according to any one of claims 1 to 9, characterized in that: The device further comprises a frame (1) and a monitor (14) arranged on the frame (1); the pipetting module (2), the liquid storage module, the ELISA plate, the purification module, the electrophoresis module (3) and the gel cutting module (4) are all arranged on the frame (1); and the monitor (14) is used to monitor the plasmid construction device.

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