Nucleic acid amplification reaction temperature control device

Abstract

The utility model belongs to the field of temperature control devices, particularly relates to a temperature control device for nucleic acid amplification reaction, and provides the following scheme aiming at the problem that the existing temperature control device for nucleic acid amplification reaction is low in heating and cooling rate: the temperature control device for nucleic acid amplification reaction comprises a box body; the thermoelectric refrigeration sheet is fixedly mounted on the box body; the fixing device is arranged on the kit body, and the fixing device is used for fixing and limiting the plurality of reagent bottles; the belt transmission mechanism is arranged on one side of the fixing device; the fan blade device is arranged on one side of the belt transmission mechanism; the electric heating wire device is arranged on one side of the fan blade device; the cooling device is arranged on one side of the kit body, and the cooling device is used for cooling the plurality of reagent bottles; the air cooling device is arranged on the cooling device; the heating and cooling rate of the temperature control device can be improved.

Description

Technical Field

[0001] The present application relates to the field of temperature control devices, and in particular to a temperature control device for nucleic acid amplification reactions. Background Art

[0002] During nucleic acid amplification reactions, the sample needs to be heated and cooled multiple times. Existing temperature control devices often use thermoelectric cooling sheets and fins to heat and cool the nucleic acid amplification reaction.

[0003] The patent document with publication number CN221217802U discloses a temperature control device for nucleic acid amplification reaction, including a temperature compensation module, a reaction module, a thermoelectric device and a heat sink stacked in sequence. The heat sink includes a plurality of fins, which are arranged in parallel along the air inlet and outlet directions to form a fin array. The fin array has a front end face, a rear end face, and a lower end face. Adjacent fins form an air duct; the air duct has a front air port, a rear air port and a lower air port. Each front air port and each rear air port has the same width and height as each other, and each lower air port has the same length and width as each other. The nucleic acid amplification instrument includes the above-mentioned temperature control device and a transformer switch. The present application can change the air inlet path in the radiator, which is beneficial to the temperature uniformity of the reaction module.

[0004] However, the existing temperature control device for nucleic acid amplification reaction has the problem of low temperature increase and decrease rate. Therefore, a temperature control device for nucleic acid amplification reaction is proposed. Utility Model Content

[0005] In order to improve the problem of low temperature rise and fall rates in existing temperature control devices for nucleic acid amplification reactions, the present application provides a temperature control device for nucleic acid amplification reactions.

[0006] The present application provides a nucleic acid amplification reaction temperature control device that adopts the following technical solution:

[0007] A nucleic acid amplification reaction temperature control device, comprising:

[0008] Box body;

[0009] Thermoelectric cooling chip, the thermoelectric cooling chip is fixedly installed on the box body;

[0010] A fixing device, the fixing device is arranged on the box body;

[0011] A belt transmission mechanism is provided on one side of the fixing device;

[0012] The fan blade device is arranged on one side of the belt transmission mechanism;

[0013] The heating wire device is arranged on one side of the fan blade device;

[0014] A cooling device is provided on one side of the box body;

[0015] The air cooling device is arranged on the temperature reduction device.

[0016] Furthermore, the fixing device includes multiple reagent bottles, multiple fixed cylinders and partitions, the partitions are fixedly connected to the box body, an insulation box is fixedly connected to the partition, a fixed frame is fixedly connected to the insulation box, multiple second openings are opened on the fixed frame, multiple reagent bottles are respectively located on the multiple second openings, multiple fixed cylinders are fixedly connected to the partitions, and the fixing device is used to fix and limit the multiple reagent bottles.

[0017] Furthermore, the belt transmission mechanism includes two pulleys and a belt, a support platform is fixedly connected to the insulation box, a motor is fixedly installed on the support platform, the output shaft of the motor is fixedly connected to one of the two pulleys, and both pulleys are connected to the belt transmission.

[0018] Furthermore, the fan blade device includes two rotating frames and two fan blades. Two first openings are opened on the insulation box. The two rotating frames are fixedly connected to the two first openings respectively. The two fan blades are rotatably connected to the two rotating frames respectively. The two fan blades are fixedly connected to the two pulleys respectively.

[0019] Furthermore, the heating wire device includes a plurality of heating wires and two ventilation tubes, the two ventilation tubes are fixedly connected to the two rotating frames respectively, and the plurality of heating wires are fixedly connected to the two ventilation tubes respectively.

[0020] Furthermore, the cooling device includes a condenser, a water pump and a water tank. The water pump is fixedly installed on the water tank. The two ends of the condenser are fixedly connected to the water tank and the water pump respectively. The condenser is located at the bottom of the partition. The cooling device is used to cool multiple reagent bottles.

[0021] Furthermore, the air cooling device includes a heat dissipation box body and an air cooling fan. The heat dissipation box body is fixedly connected to the water tank. The air cooling fan is fixedly installed on the heat dissipation box body. Two heat dissipation ports are opened on the heat dissipation box body. The air cooling device is used to cool the condensate.

[0022] In summary, this application includes at least one of the following beneficial technical effects:

[0023] 1. By starting the motor, the motor drives one of the two pulleys to rotate, and the pulley drives the other pulley to rotate through the belt, and the two pulleys respectively drive the two fan blades to rotate. When heating the nucleic acid, multiple heating wires can be started, and then a large amount of air is drawn through the fan blades and heated by the multiple heating wires to form hot air. The hot air can be evenly distributed inside the thermal insulation box to evenly heat the nucleic acid;

[0024] 2. By starting the water pump, the water pump draws condensate to the condenser tube to cool the nucleic acid. At the same time, the thermoelectric cooling plate also starts cooling. The thermoelectric cooling plate and the condensate work together to increase the cooling rate of the nucleic acid. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the overall structure of Example 1 of the present application;

[0026] Figure 2 This is a schematic diagram of the connection structure of the belt transmission mechanism, the electric wind wire device and the fan blade device in Example 1 of the present application;

[0027] Figure 3 This is a structural diagram of the belt transmission mechanism in Example 1 of the present application;

[0028] Figure 4 This is a schematic diagram of the connection structure of the fixing device, the condensing device and the air cooling device in Example 1 of the present application.

[0029] Figure numerals: 1. first opening; 2. pulley; 3. motor; 4. support platform; 5. reagent bottle; 6. second opening; 7. insulation box; 8. heat dissipation box body; 9. heat dissipation port; 10. water tank; 11. fan blades; 12. rotating frame; 13. heating wire; 14. ventilation pipe; 15. belt; 16. fixed frame; 17. fixed cylinder; 18. condenser; 19. box body; 20. partition; 21. air-cooling fan; 22. thermoelectric cooling plate; 23. water pump. DETAILED DESCRIPTION

[0030] Example 1

[0031] The following is combined with Figures 1-4 The first embodiment of the present application is described in further detail.

[0032] A nucleic acid amplification reaction temperature control device installation method reference Figure 1 ,include:

[0033] Box body 19;

[0034] Thermoelectric cooling sheet 22, the thermoelectric cooling sheet 22 is fixedly mounted on the box body 19;

[0035] A fixing device, the fixing device is provided on the box body 19;

[0036] A belt transmission mechanism is provided on one side of the fixing device;

[0037] The fan blade device is arranged on one side of the belt transmission mechanism;

[0038] The heating wire device is arranged on one side of the fan blade device;

[0039] A cooling device is provided on one side of the box body 19;

[0040] The air cooling device is arranged on the temperature reduction device.

[0041] Reference Figure 2-Figure 3 The belt transmission mechanism includes two pulleys 2 and a belt 15. A support platform 4 is fixedly connected to the insulation box 7. A motor 3 is fixedly installed on the support platform 4. The output shaft of the motor 3 is fixedly connected to one of the two pulleys 2. Both pulleys 2 are connected to the belt 15 for transmission.

[0042] The fan blade device includes two rotating frames 12 and two fan blades 11. Two first openings 1 are opened on the insulation box 7. The two rotating frames 12 are fixedly connected to the two first openings 1 respectively. The two fan blades 11 are rotatably connected to the two rotating frames 12 respectively. The two fan blades 11 are fixedly connected to the two pulleys 2 respectively.

[0043] The heating wire device includes a plurality of heating wires 13 and two ventilation pipes 14 . The two ventilation pipes 14 are fixedly connected to the two rotating frames 12 , respectively. The plurality of heating wires 13 are fixedly connected to the two ventilation pipes 14 , respectively.

[0044] Reference Figure 4 The fixing device includes multiple reagent bottles 5, multiple fixed cylinders 17 and a partition 20. The partition 20 is fixedly connected to the box body 19. The partition 20 is fixedly connected to the insulation box 7. The insulation box 7 is fixedly connected to the fixed frame 16. The fixed frame 16 is provided with multiple second openings 6. The multiple reagent bottles 5 are respectively located on the multiple second openings 6. The multiple fixed cylinders 17 are fixedly connected to the partition 20. The fixing device is used to fix and limit the multiple reagent bottles 5.

[0045] The cooling device includes a condenser 18, a water pump 23 and a water tank 10. The water pump 23 is fixedly installed on the water tank 10. The two ends of the condenser 18 are fixedly connected to the water tank 10 and the water pump 23 respectively. The condenser 18 is located at the bottom of the partition 20. The cooling device is used to cool multiple reagent bottles 5.

[0046] The cooling device includes a condenser 18, a water pump 23 and a water tank 10. The water pump 23 is fixedly installed on the water tank 10. The two ends of the condenser 18 are fixedly connected to the water tank 10 and the water pump 23 respectively. The condenser 18 is located at the bottom of the partition 20. The cooling device is used to cool multiple reagent bottles 5.

[0047] The implementation principle of a nucleic acid amplification reaction temperature control device in an embodiment of the present application is as follows: start the motor 3, the motor 3 drives one of the two pulleys 2 to rotate, the pulley 2 drives the other pulley 2 to rotate through the belt 15, and the two pulleys 2 respectively drive the two fan blades 11 to rotate. When heating the nucleic acid, multiple heating wires 13 can be started at the same time, and then a large amount of air is drawn through the fan blades 11 and heated through the multiple heating wires 13 to form hot air. The hot air can be evenly distributed inside the insulation box 7 to evenly heat the nucleic acid. When cooling the nucleic acid, start the water pump 23, and the water pump 23 draws condensate to the condenser tube 18. At the same time, the thermoelectric cooling plate 22 also starts cooling. The thermoelectric cooling plate 22 cooperates with the condensate to increase the cooling rate of the nucleic acid, and the air-cooling fan 21 can cool the condensate so that the condensate can be recycled.

[0048] Example 2

[0049] The difference between this embodiment and the first embodiment is that a temperature controller is fixedly mounted on the heat preservation box 7 , so as to control the temperature heated by the heating wire 13 and the cooling efficiency of the thermoelectric cooling plate 22 .

[0050] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A temperature control device for nucleic acid amplification reaction, characterized in that: include: Box body (19); A thermoelectric cooling sheet (22), the thermoelectric cooling sheet (22) is fixedly mounted on the box body (19); A fixing device, the fixing device being arranged on the box body (19); A belt transmission mechanism is provided on one side of the fixing device; The fan blade device is arranged on one side of the belt transmission mechanism; The heating wire device is arranged on one side of the fan blade device; A cooling device, the cooling device being arranged on one side of the box body (19); The air cooling device is arranged on the temperature reduction device.

2. A nucleic acid amplification reaction temperature control device according to claim 1, characterized in that: The fixing device comprises a plurality of reagent bottles (5), a plurality of fixed cylinders (17) and a partition (20), wherein the partition (20) is fixedly connected to the box body (19), a heat preservation box (7) is fixedly connected to the partition (20), a fixed frame (16) is fixedly connected to the heat preservation box (7), a plurality of second openings (6) are opened on the fixed frame (16), and the plurality of reagent bottles (5) are respectively located on the plurality of second openings (6), and the plurality of fixed cylinders (17) are fixedly connected to the partition (20). The fixing device is used to fix and limit the plurality of reagent bottles (5).

3. A nucleic acid amplification reaction temperature control device according to claim 2, characterized in that: The belt transmission mechanism comprises two pulleys (2) and a belt (15); a support platform (4) is fixedly connected to the heat preservation box (7); a motor (3) is fixedly mounted on the support platform (4); an output shaft of the motor (3) is fixedly connected to one of the two pulleys (2); and both pulleys (2) are connected to the belt (15) in a transmission manner.

4. A nucleic acid amplification reaction temperature control device according to claim 3, characterized in that: The fan blade device comprises two rotating frames (12) and two fan blades (11); the heat preservation box (7) is provided with two first openings (1); the two rotating frames (12) are fixedly connected to the two first openings (1), respectively; the two fan blades (11) are rotationally connected to the two rotating frames (12), respectively; and the two fan blades (11) are fixedly connected to the two pulleys (2).

5. A nucleic acid amplification reaction temperature control device according to claim 4, characterized in that: The heating wire device comprises a plurality of heating wires (13) and two ventilation pipes (14), wherein the two ventilation pipes (14) are fixedly connected to the two rotating frames (12) respectively, and the plurality of heating wires (13) are fixedly connected to the two ventilation pipes (14) respectively.

6. A nucleic acid amplification reaction temperature control device according to claim 5, characterized in that: The cooling device comprises a condenser (18), a water pump (23) and a water tank (10), wherein the water pump (23) is fixedly mounted on the water tank (10), and both ends of the condenser (18) are fixedly connected to the water tank (10) and the water pump (23), respectively. The condenser (18) is located at the bottom of the partition (20), and the cooling device is used to cool a plurality of reagent bottles (5).

7. A nucleic acid amplification reaction temperature control device according to claim 6, characterized in that: The air cooling device comprises a heat dissipation box body (8) and an air cooling fan (21), the heat dissipation box body (8) is fixedly connected to the water tank (10), the air cooling fan (21) is fixedly mounted on the heat dissipation box body (8), and two heat dissipation ports (9) are provided on the heat dissipation box body (8). The air cooling device is used to cool the condensate.

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