Cell porous culture plate special for cell slide

Abstract

The utility model relates to the technical field of cell culture, and mainly discloses a cell porous culture plate special for a cell slide, which comprises a cover plate, a plate body and a bottom plate, a plurality of culture holes are uniformly distributed on the plate body in an array manner, grooves in one-to-one correspondence with the culture holes are arranged on the bottom plate, and the bottoms of the culture holes are connected with the grooves in an embedded manner. The bottom plate is tightly connected with the side face of the bottom of the plate body, a tearing strip is arranged at the connecting position of the bottom plate and the plate body and used for tightly sealing the plate body and the plate bottom, the cover plate is arranged at the top of the plate body, the cover plate is connected with the plate body in a clamped mode, the extraction process of the cell slide can be optimized, and the operation difficulty and the risk that the cell slide is damaged are reduced. Meanwhile, the integrity and safety of cells are ensured, so that the experiment efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a multi-well cell culture plate special for a cell slide. Background Art

[0002] Multi-well cell culture plates are widely used tools in biological science research, mainly for cell culture and experimental operations. Existing multi-well cell culture plates mainly include 6-well plates, 12-well plates, 24-well plates and 96-well plates. In cell climbing experiments, researchers usually place specially prepared cell slides in multi-well cell culture plates so that cells can attach and grow on the cell slides. After completing the processing required for the experiment, researchers need to remove the cell slides from the multi-well culture plates for further observation and analysis. They are widely used in experimental techniques such as immunofluorescence and in situ hybridization.

[0003] However, current multi-well culture plates present significant operational difficulties when gripping cell slides. Due to the height limitations of multi-well culture plates and the surface tension between the cell slide and the bottom of the culture plate, researchers often encounter difficulties in gripping the cell slides, resulting in easy breakage of the cell slides or destruction of the cell layer during extraction, thus affecting experimental results and subsequent experimental progress. Utility Model Content

[0004] The purpose of this utility model is to solve the technical problems existing in the prior art and provide a cell multi-well culture plate specifically for cell slides. This plate can optimize the extraction process of cell slides, reduce the difficulty of operation and the risk of cell slide damage. At the same time, it ensures the integrity and safety of cells, thereby improving experimental efficiency and meeting the practical needs of modern biomedical research.

[0005] To achieve the above-mentioned purpose, the utility model adopts the following technical solutions: a cell multi-porous culture plate specially used for cell slides, comprising a cover plate, a plate body and a bottom plate, wherein a plurality of culture wells are evenly distributed in an array on the plate body, and grooves corresponding to the culture wells are provided on the bottom plate, and the bottoms of the culture wells are embedded in the grooves, and the bottom plate is tightly connected to the side of the bottom of the plate body, and a tear strip is provided at the connection between the bottom plate and the plate body, and the tear strip is used to tightly seal the plate body and the bottom of the plate to ensure that the connection between the two is in a completely sealed state, thereby avoiding the risk of leakage during the cell culture process, and the cover plate is arranged on the top of the plate body, and the cover plate is snap-connected to the plate body to ensure a sterile environment during the cell culture process.

[0006] Furthermore, the plate body is in a rectangular parallelepiped shape, the upper parts of the plurality of culture holes are flush with the top of the plate body, and the lower parts of the plurality of culture holes extend beyond the bottom of the plate body by about 4-6 mm.

[0007] Furthermore, the bottom plate is in a rectangular shape, and the depth of the groove is 2-3 mm.

[0008] Furthermore, the width of the tear strip is 2-3 mm.

[0009] Furthermore, there is an interference fit between the bottom of the culture well and the groove.

[0010] Furthermore, a locking portion is provided at the bottom of the plate body, and an embedded portion is provided above the bottom plate. Preferably, the embedded portion is a rectangular annular protrusion with rounded corners, the locking portion is a rectangular annular protrusion with rounded corners, the embedded portion is embedded in the locking portion, and the embedded portion and the locking portion are in interference connection.

[0011] Furthermore, a tear portion is provided at one end of the tear strip.

[0012] Preferably, the tearing portion is in the shape of a ring or a convex piece.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] The plate and base of this multi-well cell culture plate are not only connected by the cell culture wells in the plate body and the grooves in the base, but also tightly connected by a tear strip, enabling routine cell slicing experiments and subsequent processing without leakage. Once the cell slicing experiment is complete, researchers can separate the plate body from the base by pulling the tear strip and easily pick up the cell slide in the groove of the base using tweezers. This design significantly improves operational convenience and work efficiency while reducing the risk of damage during the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0016] Figure 1 This is a schematic diagram of the explosion structure of the multi-well cell culture plate of the utility model;

[0017] Figure 2 This is a schematic structural diagram of the multi-well cell culture plate of the present invention with the cover removed;

[0018] Figure 3 This is a schematic diagram of the plate structure of the multi-well cell culture plate of the present invention;

[0019] Figure 4 This is a schematic diagram of the bottom plate structure of the multi-well cell culture plate of the present invention;

[0020] Figure 5 This is a schematic diagram of the overall structure of the multi-well cell culture plate of the present invention.

[0021] In the figure: 1, cover plate; 2, plate body; 3, bottom plate; 4, culture well; 5, groove; 6, tear strip; 7, tear part; 8, locking part; 9, embedding part; 10, cell slide. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0023] like Figure 1-5 As shown, a cell multi-well culture plate specially used for cell slides includes a cover plate 1, a plate body 2 and a bottom plate 3.

[0024] The plate body 2 is rectangular in shape, and is evenly arrayed with a plurality of culture wells 4 on the plate body 2. The number of culture wells 4 is common, such as 6 wells, 12 wells, 24 wells, 48 ​​wells, and 96 wells. Taking 12 wells as an example, the array is 3*4. Specifically, the plate body 2 includes a top plate and a bottom plate 3. The culture wells 4 are formed as cylindrical plates with openings at both ends. The tops of the plurality of culture wells 4 are flush with the top plate of the plate body 2, and the bottoms of the plurality of culture wells 4 extend beyond the bottom plate 3 of the plate body 2 by approximately 4-6 mm, preferably by 4 mm.

[0025] The base plate 3 is cuboid, specifically, rectangular. A groove 5 is provided on the base plate 3, corresponding one-to-one with each culture well 4. The groove 5 has a depth of 2-3 mm, preferably 2 mm. The bottom of the culture well 4 is embedded in the groove 5. Furthermore, the bottom of the culture well 4 and the groove 5 are interference-fitted. This allows for precise fit between the cell culture well 4 and the groove 5 of the base plate 3, ensuring stability.

[0026] Furthermore, the bottom plate 3 of the plate body 2 is provided with a snap-fit ​​portion 8, and the top of the bottom plate 3 is provided with an embedded portion 9. Preferably, the embedded portion 9 is a rectangular annular protrusion with rounded corners, and the snap-fit ​​portion 8 is a rectangular annular protrusion with rounded corners. The embedded portion 9 is embedded in the snap-fit ​​portion 8, and the embedded portion 9 and the snap-fit ​​portion 8 are in interference fit. The tear strip 6 is provided between the bottom edge of the snap-fit ​​portion 8 and the top edge of the bottom plate 3. The tear strip 6 is used to tightly seal the plate body 2 and the bottom of the plate, ensuring that the connection between the two is in a completely sealed state, thereby avoiding the risk of leakage during cell culture.

[0027] The manufacturing process of the tear strip 6 to achieve a tight connection between the plate body 2 and the bottom plate 3 is prior art, and reference is made to a chewing gum box with a tear strip 6 .

[0028] Furthermore, one end of the tear strip 6 is provided with a tear portion 7. Furthermore, the width of the tear strip 6 is 2-3 mm, preferably 2 mm. Preferably, the tear portion 7 is in the shape of a ring or a tab. This allows the operator to easily pull open the tear strip 6 after the experiment to separate the plate body 2 from the base plate 3.

[0029] The cover plate 1 is in a rectangular parallelepiped shape and is disposed on top of the plate body 2 . The cover plate 1 is engaged with the plate body 2 to ensure a sterile environment during cell culture.

[0030] The connection method between the cover plate 1 and the plate body 2 is the existing technology. One corner end of the cover plate 1 and the plate body 2 is chamfered, and a card groove is provided on the edge above the outer wall of the plate body 2, and a corresponding card groove is provided on the lower part of the inner wall of the cover plate 1, so that the cover plate 1 and the plate body 2 are tightly connected.

[0031] Working method:

[0032] When conducting an experiment, the cover plate 1 is opened, and the cell slide 10 is placed in the culture well 4 with tweezers. The cell slide 10 is placed on the bottom surface of the groove 5. The bottom of the culture well 4 is embedded in the groove 5 for interference connection, and the tear strip 6 tightly seals the base plate 3 and the plate body 2, so that no leakage occurs when conducting a routine experiment. Thereafter, the cover plate 1 is covered, and the cover plate 1 is tightly sealed with the plate body 2 to ensure the sealing conditions during the experiment. After the experiment is completed, the tear strip 6 is torn open by the tearing portion 7, the plate body 2 and the base plate 3 are separated, and the plate body 2 is removed, so that the shallower groove 5 is exposed to the outside, and the cell slide 10 is easily clamped with tweezers for subsequent observation.

[0033] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the present invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be encompassed within the present invention. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

Claims

1. A multi-well cell culture plate dedicated to a cell slide, characterized by: The invention comprises a cover plate (1), a plate body (2) and a bottom plate (3); a plurality of culture wells (4) are evenly distributed in an array on the plate body (2); a groove (5) corresponding to the culture wells (4) is provided on the bottom plate (3); the bottom of the culture well (4) is embedded in the groove (5); the bottom of the bottom plate (3) is tightly connected to the side of the bottom of the plate body (2); a tear strip (6) is provided at the connection between the bottom plate (3) and the plate body (2); the tear strip (6) is used to tightly seal the plate body (2) and the bottom of the plate; the cover plate (1) is arranged on the top of the plate body (2); and the cover plate (1) is snap-fitted to the plate body (2).

2. The multi-well cell culture plate for cell slides according to claim 1, characterized in that: The plate body (2) is in the shape of a rectangular parallelepiped, the upper sides of the plurality of culture holes (4) are flush with the top of the plate body (2), and the lower sides of the plurality of culture holes (4) extend beyond the bottom of the plate body (2) by about 4-6 mm.

3. The multi-well cell culture plate for cell slides according to claim 2, characterized in that: The bottom plate (3) is in the shape of a cuboid, and the depth of the groove (5) is 2-3 mm.

4. The multi-well cell culture plate for cell slides according to claim 3, characterized in that: The width of the tear strip (6) is 2-3 mm.

5. A multi-well cell culture plate for cell slides according to any one of claims 1 to 4, characterized in that: The bottom of the culture hole (4) and the groove (5) are in interference fit connection.

6. The multi-well cell culture plate for cell slides according to claim 5, characterized in that: The bottom of the plate body (2) is provided with a snap-fitting portion (8), and the upper portion of the bottom plate (3) is provided with an embedded portion (9). The embedded portion (9) is a rectangular annular protrusion with rounded corners. The snap-fitting portion (8) is a rectangular annular protrusion with rounded corners. The embedded portion (9) is embedded in the snap-fitting portion (8), and the embedded portion (9) and the snap-fitting portion (8) are in interference fit.

7. The multi-well cell culture plate for cell slides according to claim 6, characterized in that: One end of the tear strip (6) is provided with a tear portion (7).

8. The multi-well cell culture plate for cell slides according to claim 7, characterized in that: The tearing portion (7) is in the shape of a ring or a convex piece.

Images