Multi-channel fluorescence imaging system

Abstract

The utility model belongs to the technical field of detection, and particularly relates to a multichannel fluorescence imaging system which comprises a camera, a light source group, a sample plate and three sets of optical filters, the sample plate can be a flat plate, a 6-pore plate, a 24-pore plate, a 96-pore plate, a 384-pore plate, a 1596-pore plate or pore plates with other specifications; the camera is fixedly connected with a lens, and the camera is used for shooting a sample on the sample plate; the light source group is used for exciting fluorescent dye coupled with a sample in the sample plate, the fluorescent dye can be one or more, the wavelengths of three kinds of light are different, and the light source can be replaced. According to the utility model, the three fluorescent dye samples are respectively shot and detected in three different exciting light environments, so that the detection accuracy can be greatly improved.

Description

Technical Field

[0001] The utility model belongs to the field of detection technology, and particularly relates to a multi-channel fluorescence imaging system. Background Art

[0002] In fields such as biomedical testing and chemical analysis, the accurate detection and analysis of different substances has always been a research focus and difficulty. With the continuous deepening of scientific research and the increasing diversification of practical application scenarios, the requirements for detection technology are becoming more and more stringent.

[0003] Traditional detection methods can determine the type of target by photographing the fluorescent dye coupled to the target sample. However, since the fluorescent dye coupled to the target sample is not fully excited during the photographing process, the fluorescent dye on the sample is not clear after the camera takes the image, which greatly affects the accuracy of the detection. Utility Model Content

[0004] The purpose of the utility model is to provide a multi-channel fluorescence imaging system, which can photograph and detect samples of three fluorescent dyes respectively under three different excitation light environments, so as to more clearly photograph the fluorescent dyes on the samples and greatly improve the accuracy of detection.

[0005] The technical solutions adopted by this utility model are as follows:

[0006] A multi-channel fluorescence imaging system includes a camera, a light source group, a sample plate, and three filters;

[0007] The camera is fixedly connected to a lens, and the camera is used to photograph the sample on the sample plate;

[0008] The light source group is used to excite the fluorescent dyes marked on the sample. The light source group irradiates three lights respectively. The three lights have different wavelengths and are respectively adapted to the three fluorescent dyes.

[0009] The three filters are respectively used to filter the three colors of light from the light source group. The colors of the three filters are respectively matched with the three fluorescent dyes. The three filters can be moved in sequence to the side of the lens facing the sample plate.

[0010] Furthermore, the sample plate is any one of a flat plate, a 6-well plate, a 24-well plate, a 96-well plate, a 384-well plate, and a 1596-well plate.

[0011] Furthermore, the light source group includes three groups of light sources, and the three groups of light sources respectively excite light of three wavelengths.

[0012] Furthermore, the multi-channel fluorescence imaging system also includes a horizontal moving device, to which a mounting plate is fixedly connected. The horizontal moving device can drive the mounting plate to move on the upper side of the lens, and the three filters are all fixedly connected to the mounting plate.

[0013] Furthermore, the horizontally moving device includes two side rails, the mounting plate is horizontally slidably connected between the two side rails, a tooth groove is opened on the upper side of the mounting plate, a servo motor is fixedly connected to one of the side rails, and the output end of the servo motor is fixedly connected to a meshing gear meshing with the tooth groove.

[0014] The technical effects achieved by this utility model are:

[0015] The multi-channel fluorescence imaging system of the utility model can photograph and detect samples of three fluorescent dyes respectively under three different excitation light environments, so as to more clearly photograph the fluorescent dyes on the samples and greatly improve the accuracy of detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural diagram of the utility model;

[0017] Figure 2 It is a schematic diagram of the utility model;

[0018] Figure 3 It is a structural diagram of the mounting plate of the utility model;

[0019] Figure 4 It is a structural schematic diagram of the sample plate of the utility model.

[0020] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0021] 1. Camera; 2. Light source assembly; 3. Sample plate; 4. Sample storage hole; 5. Sample; 6. Lens; 7. Filter; 8. Mounting plate; 9. Side slide rail; 10. Servo motor; 11. Gear; 12. Gear groove. DETAILED DESCRIPTION

[0022] In order to make the purpose and advantages of the present invention more clearly understood, the present invention is described in detail below with reference to the following embodiments. It should be understood that the following text is only used to describe one or several specific embodiments of the present invention and does not strictly limit the scope of protection of the present invention.

[0023] like Figure 1 As shown, a multi-channel fluorescence imaging system includes a camera 1, a light source group 2, a sample plate 3, and three filters 7;

[0024] Among them, Figure 1 As shown, samples 5 are stored on the sample plate 3 , and different samples 5 are coupled and labeled with different fluorescent dyes. The types of fluorescent dyes set on the samples 5 can be one or more, preferably three.

[0025] The sample plate 3 is preferably any one of a flat plate, a 6-well plate, a 24-well plate, a 96-well plate, a 384-well plate, and a 1596-well plate, that is, a sample storage well 4 can be opened on the sample plate 3, and the number of the sample storage wells 4 can be 1, 6, 24, 96, 384, or 1536, which can be selected according to the needs of the user, to meet more needs of the user and complete imaging of various specifications;

[0026] Meanwhile, the sample plate 3 may also be a well plate of other specifications.

[0027] like Figure 1 As shown, a lens 6 is fixedly connected to the upper side of the camera 1 , and the sample plate 3 is preferably located on the upper side of the lens 6 . The camera 1 is used to photograph the sample 5 on the sample plate 3 , that is, to photograph the fluorescent dye of the sample 5 .

[0028] Wherein, the light source group 2 is used to excite the fluorescent dye marked on the sample 5;

[0029] The light source group 2 irradiates three kinds of lights respectively. The three kinds of lights have different wavelengths. The three kinds of lights are respectively adapted to the three kinds of fluorescent dyes on the sample 5 .

[0030] Further, such as Figure 1-2 As shown, the light source group 2 includes three groups of light sources, and the light sources are replaceable. The three groups of light sources respectively excite light of three wavelengths. At this time, the sample 5 is irradiated by starting the three groups of light sources in sequence, and the camera 1 is started respectively to take pictures.

[0031] like Figure 1-3 As shown, the three filters 7 are adapted to the light source group 2 and can be moved in sequence to the side of the lens 6 facing the sample plate 3. The lens 6 of the camera 1 shoots the sample 5 through the corresponding filters 7. Figure 2 As shown, Figure 2 The dotted area in is the effective shooting area of ​​camera 1.

[0032] The three filters 7 are respectively used to filter the three colors of light from the light source group 2. The colors of the three filters 7 are respectively matched with the three fluorescent dyes, so that the matched fluorescent dyes are highlighted. That is, when the first filter 7 is assembled on the upper side of the lens 6, the camera 1 can more clearly photograph the first fluorescent dye on the sample 5; when the second filter 7 is assembled on the upper side of the lens 6, the camera 1 can more clearly photograph the second fluorescent dye on the sample 5; when the third filter 7 is assembled on the upper side of the lens 6, the camera 1 can more clearly photograph the third fluorescent dye on the sample 5.

[0033] like Figure 1-3 As shown, in order to be able to move the filter 7 more conveniently, the multi-channel fluorescence imaging system also includes a horizontal moving device, to which a mounting plate 8 is fixedly connected. The horizontal moving device can drive the mounting plate 8 to move on the upper side of the lens 6. The three filters 7 are all fixedly connected to the mounting plate 8. At this time, by starting the horizontal moving device to drive the mounting plate 8 to move, the positions of the three filters 7 can be quickly adjusted, and the corresponding filters 7 can be assembled on the upper side of the lens 6.

[0034] like Figure 1-3 As shown, the horizontally moving device includes two side rails 9, and the mounting plate 8 is horizontally slidably connected between the two side rails 9. A tooth groove 12 is provided on the upper side of the mounting plate 8. A servo motor 10 is fixedly connected to one of the side rails 9, and the output end of the servo motor 10 is fixedly connected to a meshing gear 11 that is meshed with the meshing groove 12. At this time, by starting the servo motor 10 to drive the meshing gear 11 to rotate, the mounting plate 8 can be pushed through the meshing groove 12, driving the mounting plate 8 and the filter 7 to move horizontally together, and the moving method is relatively simple.

[0035] The horizontal moving device may also be an electric push rod or other device capable of driving the mounting plate 8 to move horizontally.

[0036] The working principle of this utility model is:

[0037] Start the servo motor 10 to drive the filter 7 to move, so that the three filters 7 move to the upper side of the lens 6 in turn, and at the same time start the corresponding lamps to illuminate the sample 5, and then start the camera 1 to shoot. By shooting and detecting the fluorescent dye on the sample 5 respectively under three light environments, the fluorescent dye on the sample 5 can be effectively stimulated, so that the fluorescent dye on the sample 5 can be shot more clearly, greatly improving the accuracy of the detection.

[0038] The above description is merely a preferred embodiment of the present invention. It should be noted that those skilled in the art may make various improvements and modifications without departing from the principles of the present invention, and such improvements and modifications should be considered within the scope of protection of the present invention. Structures, devices, and operating methods not specifically described or explained in this invention shall, unless otherwise specified or limited, be implemented in accordance with conventional means in the art.

Claims

1. A multi-channel fluorescence imaging system, characterized in that: It includes a camera (1), a light source group (2), a sample plate (3), and three filters (7); A lens (6) is fixedly connected to the camera (1), and the camera (1) is used to photograph the sample (5) on the sample plate (3); The light source group (2) is used to excite the fluorescent dyes marked on the sample (5), and the light source group (2) irradiates three kinds of light respectively, and the three kinds of light have different wavelengths, and the three kinds of light wavelengths are respectively adapted to the three kinds of fluorescent dyes; The three filters (7) are respectively used to filter the three-color light of the light source group (2). The colors of the three filters (7) are respectively adapted to the three fluorescent dyes. The three filters (7) can be moved in sequence on the side of the lens (6) facing the sample plate (3).

2. A multi-channel fluorescence imaging system according to claim 1, characterized in that: The sample plate (3) is any one of a flat plate, a 6-well plate, a 24-well plate, a 96-well plate, a 384-well plate, and a 1596-well plate.

3. A multi-channel fluorescence imaging system according to claim 1, characterized in that: The sample plate (3) is located on the upper side of the lens (6), and the lens (6) is fixedly connected to the upper side of the camera (1).

4. The multi-channel fluorescence imaging system according to claim 1, wherein: The light source group (2) comprises three groups of light sources, and the three groups of light sources respectively excite light of three wavelengths.

5. The multi-channel fluorescence imaging system according to claim 1, characterized in that: The multi-channel fluorescence imaging system further comprises a horizontal moving device, to which a mounting plate (8) is fixedly connected. The horizontal moving device can drive the mounting plate (8) to move on the upper side of the lens (6), and the three filters (7) are all fixedly connected to the mounting plate (8).

6. The multi-channel fluorescence imaging system according to claim 5, characterized in that: The horizontally movable device comprises two side rails (9), the mounting plate (8) is horizontally slidably connected between the two side rails (9), a meshing groove (12) is provided on the upper side of the mounting plate (8), a servo motor (10) is fixedly connected to one of the side rails (9), and an output end of the servo motor (10) is fixedly connected to a meshing gear (11) meshing with the meshing groove (12).

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