Adsorption type biological matrix treatment instrument

Abstract

The utility model relates to the technical field of biological matrix treatment instruments, in particular to an adsorption type biological matrix treatment instrument which comprises a box body, a liquid supply cavity and an experiment cavity are arranged in the box body, a chromatographic column is arranged in the experiment cavity, a plunger pump for supplying liquid to the chromatographic column is arranged in the liquid supply cavity, the plunger pump is communicated with a plurality of pressure pumps, and liquid inlets of the pressure pumps are communicated with a plurality of liquid storage tanks. A control screen for controlling the pressure pump and the plunger pump is arranged on the box body, and at least one adjusting piece is arranged in a connecting pipe between the plunger pump and the chromatographic column. Therefore, liquid can be automatically added into the chromatographic column, the labor cost is reduced, the working efficiency is improved, and in the liquid adding process, not only can new impurities be prevented from being introduced, but also reflux can be prevented.

Description

Technical Field

[0001] The utility model relates to the technical field of biological matrix processing instruments, in particular to an adsorption-type biological matrix processing instrument. Background Art

[0002] Chromatographic separation, also known as chromatography, utilizes the differences in the physical and chemical properties of the components in a sample to distribute them to varying degrees in two phases. One phase is fixed (called the stationary phase), while the other phase flows through this stationary phase (called the mobile phase) and causes the components to move at different speeds, thereby achieving separation. Chromatography is often required in the process of biological matrix processing.

[0003] At present, the existing chromatographic separation device, such as the Chinese patent with publication number CN219897134U, discloses an automatic column passing device, including a base, a controller, a fixing frame, a chromatography column, a liquid adding device, and a peristaltic pump; the base is arranged horizontally; the controller is arranged on the left side of the top of the base; the fixing frame is arranged vertically on the right rear side of the controller, and a U-shaped buckle is provided on the front side of the top of the fixing frame; the opening of the U-shaped buckle faces left; the chromatography column is clamped in the U-shaped buckle, and its liquid outlet faces downward; the liquid adding device includes a robotic arm and a liquid adding head; the robotic arm is arranged above the fixing frame, and the rear The bottom of the end is connected to a lifting and movable bracket, which is arranged behind the fixed bracket and connected to the controller; the liquid adding head is arranged at the bottom of the front side of the robotic arm, and the robotic arm can drive the liquid adding head to move to the top of the chromatography column to add liquid; the peristaltic pump is arranged on the base and electrically connected to the controller, its discharge end is connected to the liquid adding head, and the feed end is connected to the solvent barrel. The whole process is basically automated, which can replace manual column passing and reduce labor costs. However, the above-mentioned device has a complex structure and poor sealing. It is easy to introduce new impurities during operation, and it is inconvenient to use in practice.

[0004] How to automatically add liquid to the chromatography column, reduce labor costs, improve work efficiency, and avoid the introduction of new impurities and prevent backflow during the addition process has become a technical problem that needs to be broken through.

[0005] In summary, the existing technology has obvious inconveniences and defects in actual use, so it is necessary to improve it. Utility Model Content

[0006] In response to the above-mentioned shortcomings, the purpose of the present invention is to provide an adsorption-type biological matrix processing instrument with a simple structure that can automatically add liquid to the chromatography column, reducing labor costs and improving work efficiency. During the liquid addition process, it can not only avoid the introduction of new impurities but also prevent backflow.

[0007] To achieve the above-mentioned objectives, the present invention provides an adsorption-type biological matrix processing instrument, comprising a housing, wherein a liquid supply chamber and an experimental chamber are provided within the housing, a chromatography column is provided within the experimental chamber, a plunger pump is provided within the liquid supply chamber for supplying liquid to the chromatography column, the plunger pump is connected to a plurality of pressure pumps, the liquid inlet of the pressure pump is connected to a plurality of liquid storage tanks, and a control panel for controlling the pressure pump and the plunger pump is provided on the housing.

[0008] At least one regulating member is provided in the connecting pipe between the plunger pump and the chromatography column.

[0009] According to the adsorption-type biological matrix processing instrument of the present invention, the regulating member is an annular structure, and the inner circumference of the regulating member is provided with a plurality of check plates inclined in the liquid supply direction, and gaps are provided between the check plates.

[0010] According to the adsorption-type biological matrix processing instrument of the present invention, the number of the check plates is 12, and the 12 check plates are evenly distributed along the inner circumference of the adjusting member.

[0011] According to the adsorption-type biological matrix processing instrument of the present invention, a fixing mechanism is provided between the chromatography column and the housing, and the fixing mechanism includes a fixing bar fixed to the housing, a driving rod is rotatably connected to the fixing bar, one end of the driving rod passes through the fixing bar, and sliding blocks are symmetrically threaded on the driving rod inside the fixing bar, one end of each sliding block passes through the fixing bar, and the end of each sliding block passing through the fixing bar is connected to a fixing clip.

[0012] According to the adsorption-type biological matrix processing instrument of the present invention, the fixing clamps are all clamped on the outside of the chromatography column, and the fixing clamps include two clamping blocks fixed to the sliding block, and a fixing rod is threaded through one of the clamping blocks.

[0013] According to the adsorption-type biological matrix processing instrument of the present invention, several liquid storage tanks for supplying liquid to the pressure pump are all arranged in the experimental chamber, a liquid storage tank for collecting is provided below the chromatography column, and the same tray is provided below the several liquid storage tanks for supplying liquid to the pressure pump and the liquid storage tank for collecting.

[0014] According to the adsorption-type biological matrix processing instrument of the present invention, one side of the liquid supply chamber and the experimental chamber are both open, and movable doors are hinged at the openings of the liquid supply chamber and the experimental chamber.

[0015] The purpose of the present utility model is to provide an adsorption-type biological matrix processing instrument, including a liquid supply chamber and an experimental chamber, wherein the plunger pump and the pressure pump and other structures in the liquid supply chamber cooperate with each other to realize automatic liquid addition to the chromatography column, reduce labor costs, improve work efficiency, and avoid the introduction of new impurities during the liquid addition process; by rotating the driving rod of the fixing mechanism, the chromatography column can be driven to rise and fall, thereby adjusting the height of the chromatography column; at least one adjusting member is provided in the connecting pipe between the plunger pump and the chromatography column, which can prevent backflow during the liquid addition process. In summary, the beneficial effects of the present utility model are: it can realize automatic liquid addition to the chromatography column, reduce labor costs, improve work efficiency, and avoid the introduction of new impurities and prevent backflow during the liquid addition process. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is the structural diagram of the utility model;

[0017] Figure 2 It is a structural diagram of the adjustment part;

[0018] Figure 3 is a cross-sectional view of the adjusting member;

[0019] Figure 4 It is a cross-sectional view of the driving rod;

[0020] In the figure: 1-box, 11-liquid supply chamber, 12-experimental chamber, 13-movable door, 14-control panel, 2-pressurization pump, 3-plunger pump, 4-fixing mechanism, 41-fixing bar, 42-driving rod, 43-sliding block, 44-fixing clamp, 5-chromatographic column, 6-connecting tube, 7-liquid storage tank, 71-tray, 8-adjusting part, 81-check piece. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0022] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top / bottom" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they cannot be understood as limitations on the present invention.

[0023] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "sleeved / connected", "connected", etc. should be understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection. It can be a mechanical connection or an electrical connection. It can be a direct connection or an indirect connection through an intermediate medium. It can be a connection between the two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0024] See also Figures 1 to 4 The present invention provides an adsorption-type biological matrix processing instrument, including a housing 1, a liquid supply chamber 11 and an experimental chamber 12 are provided in the housing 1, and one side of the liquid supply chamber 11 and the experimental chamber 12 are both open. A chromatography column 5 is provided in the experimental chamber 12 (the structure of the chromatography column 5 is the same as that of the prior art, and its specific structure and working principle are not repeated here). A plunger pump 3 for supplying liquid to the chromatography column 5 is provided in the liquid supply chamber 11, and the plunger pump 3 is connected to a plurality of pressure pumps 2, and the liquid inlet of the pressure pump 2 is connected to a plurality of liquid storage tanks 7 (such as beakers). The plurality of liquid storage tanks 7 connected to the pressure pump 2 are used to hold a plurality of different liquids. Between the plunger pump 3 and the chromatography column 5, a plurality of Connecting pipes 6 are provided between the pressure pump 2 and the plunger pump 3, and between several pressure pumps 2 and several liquid storage tanks 7 (using connecting pipes 6 to connect several devices that need to be connected is a mature existing technology in this field, and the specific connection method will not be repeated here). A control panel 14 is provided on the box body 1, and a controller is provided inside the box body 1. The controller is electrically connected to the control panel 14, the pressure pump 2 and the plunger pump 3. The pressure pump 2 and the plunger pump 3 can be uniformly regulated by the controller (using the control panel 14 to control the controller, thereby regulating the electrical equipment is a mature existing technology in this field, and its specific electrical connection method and working principle will not be repeated here).

[0025] See also Figures 1 to 4 Preferably, the openings of the liquid supply chamber 11 and the experimental chamber 12 are hinged with movable doors 13. When there is no need to take or place items, the movable doors 13 can be closed to prevent external dust and other impurities from entering the liquid supply chamber 11 and the experimental chamber 12.

[0026] See also Figures 1 to 4Furthermore, at least one adjusting member 8 is provided in the connecting pipe 6 between the plunger pump 3 and the chromatography column 5 (the opening at the top of the chromatography column 5 is connected to a T-shaped three-way hose connector, and a rubber band is tied between the T-shaped three-way hose connector and the chromatography column 5 to enhance airtightness and stability. One end of the connecting pipe 6 between the plunger pump 3 and the chromatography column 5 is connected to the T-shaped three-way hose connector, and the other end of the T-shaped three-way hose connector is connected to the plunger pump 3). The adjusting member 8 is an annular structure, and the inner periphery of the adjusting member 8 is provided with a plurality of check pieces 81 inclined in the liquid supply direction, and gaps are provided between the plurality of check pieces 81 to prevent backflow of liquid in the connecting pipe 6 between the plunger pump 3 and the chromatography column 5.

[0027] See also Figures 1 to 4 Preferably, the number of the check plates 81 is 12, and the 12 check plates 81 are evenly distributed along the inner circumference of the adjusting member 8.

[0028] See also Figures 1 to 4 The chute 5 is fixed to the outer surface of the chute 1 and the outer surface of the chute 1 is fixed to the outer surface of the chute 1. The chute 5 is fixed to the outer surface of the chute 1 and the inner surface of the chute 1 is fixed to the outer surface of the chute 1. The chute 5 is fixed to the outer surface of the chute 1 and the outer surface of the chute 1 is fixed to the outer surface of the chute 1.

[0029] See also Figures 1 to 4 Preferably, the several liquid storage tanks 7 for supplying liquid to the pressure pump 2 are all arranged in the experimental cavity 12 (connecting pipes 6 are provided between the pressure pump 2 and the several liquid storage tanks 7 and penetrate into the experimental cavity 12), and a liquid storage tank 7 for collecting is provided below the chromatography column 5. The same tray 71 is provided below the several liquid storage tanks 7 for supplying liquid to the pressure pump 2 and the liquid storage tank 7 for collecting, which is convenient for taking the several liquid storage tanks 7 for supplying liquid to the pressure pump 2 and the liquid storage tank 7 for collecting.

[0030] See also Figures 1 to 4, place several liquid storage tanks 7 for supplying liquid to the booster pump 2 and the liquid storage tanks 7 for collecting liquid into the experimental chamber 12, wherein the several liquid storage tanks 7 for supplying liquid to the booster pump 2 are connected to the connecting pipe 6 between the booster pump 2 and the several liquid storage tanks 7, and the liquid storage tank 7 for collecting is placed under the chromatography column 5, and the control panel 14 can be used to control the plunger pump 3 and the several booster pumps 2 to perform experimental operations.

[0031] The present invention provides an adsorption-type biological matrix processing instrument, including a liquid supply chamber and an experimental chamber, wherein the plunger pump and the pressure pump and other structures in the liquid supply chamber cooperate with each other to realize automatic liquid addition to the chromatography column, reduce labor costs, improve work efficiency, and avoid the introduction of new impurities during the liquid addition process; by rotating the driving rod of the fixing mechanism, the chromatography column can be driven to rise and fall, thereby adjusting the height of the chromatography column; at least one adjusting member is provided in the connecting pipe between the plunger pump and the chromatography column, which can prevent backflow during the liquid addition process. In summary, the beneficial effects of the present invention are: it can realize automatic liquid addition to the chromatography column, reduce labor costs, improve work efficiency, and avoid the introduction of new impurities and prevent backflow during the liquid addition process.

[0032] Of course, the present invention may have many other embodiments. Without departing from the spirit and essence of the present invention, technicians familiar with the field can make various corresponding changes and modifications based on the present invention, but these corresponding changes and modifications should all fall within the scope of protection of the claims attached to the present invention.

Claims

1. An adsorption-type biological matrix processing instrument, characterized in that: The invention comprises a box body, wherein a liquid supply chamber and an experimental chamber are provided in the box body, a chromatography column is provided in the experimental chamber, a plunger pump for supplying liquid to the chromatography column is provided in the liquid supply chamber, the plunger pump is connected to a plurality of pressure pumps, the liquid inlet of the pressure pump is connected to a plurality of liquid storage tanks, and a control panel for controlling the pressure pump and the plunger pump is provided on the box body; At least one regulating member is provided in the connecting pipe between the plunger pump and the chromatography column.

2. The adsorption-type biological matrix processing instrument according to claim 1, characterized in that The regulating member is an annular structure. The inner periphery of the regulating member is provided with a plurality of check plates inclined toward the liquid supply direction, and gaps are provided between the check plates.

3. The adsorption-type biological matrix processing instrument according to claim 2, characterized in that: The number of the check plates is 12, and the 12 check plates are evenly distributed along the inner circumference of the adjusting member.

4. The adsorption-type biological matrix processing instrument according to claim 1, characterized in that A fixing mechanism is provided between the chromatography column and the box body, and the fixing mechanism includes a fixing bar fixed to the box body, a driving rod is rotatably connected to the fixing bar, one end of the driving rod passes through the fixing bar, and sliding blocks are symmetrically threaded on the driving rod inside the fixing bar, one end of the sliding blocks passes through the fixing bar, and the ends of the sliding blocks passing through the fixing bar are connected to fixing clips.

5. The adsorption-type biological matrix processing instrument according to claim 4, characterized in that: The fixing clamps are all clamped on the outside of the chromatography column, and the fixing clamps include two clamping blocks fixed to the sliding block, wherein a fixing rod is threadedly passed through one of the clamping blocks.

6. The adsorption-type biological matrix processing instrument according to claim 1, characterized in that: Several liquid storage tanks for supplying liquid to the pressure pump are all arranged in the experimental cavity, a liquid storage tank for collecting is provided below the chromatography column, and the same tray is provided below the several liquid storage tanks for supplying liquid to the pressure pump and the liquid storage tank for collecting.

7. The adsorption-type biological matrix processing instrument according to claim 1, characterized in that: One side of the liquid supply chamber and the experimental chamber is open, and movable doors are hinged at the openings of the liquid supply chamber and the experimental chamber.

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