Anti-pollution bacterial culture device convenient for sample adding and sampling

Abstract

The anti-pollution bacterial culture device comprises a culture container body, a tray and a cover, the tray is arranged at an opening of the culture container body, the cover is used for sealing the opening of the culture container body, a groove is formed in the tray, liquid absorption materials are arranged in the groove, and a sample adding opening is formed in the tray; the cover is an elastic sealing cover, a plurality of elastic self-sealing holes with different hole diameters are formed in the cover, and the elastic self-sealing holes are in a sealed state under the action of elastic materials of the elastic self-sealing holes when no external force exists. The device provided by the utility model can effectively prevent the pollution of external infectious microbes, improve the accuracy and reliability of experimental results, and avoid the reuse of the used elastic self-sealing hole at the same time.

Description

Technical Field

[0001] The utility model relates to a biological experiment device, in particular to a bacteria culture device which is pollution-proof and convenient for adding and taking samples. Background Art

[0002] Bacterial culture is a common basic experiment in multidisciplinary experimental teaching and clinical testing such as medicine and molecular biology. During the bacterial culture process, special attention should be paid to preventing contamination by foreign bacteria. However, from culturing bacteria to harvesting bacteria, multiple processes are often involved. For example, when measuring the bacterial growth concentration, it is necessary to regularly aspirate the bacterial solution from the culture bottle and measure the OD value; when using bacteria as hosts for exogenous gene expression, it is necessary to add inducers; and to meet different experimental purposes, drugs and the like are also added. In current experiments, all of the above operations require the bacterial culture container to be completely opened and placed in a sterile clean bench for operation. In this process, contamination may occur due to incomplete environmental sterilization or improper operation of the experimenter, resulting in failure of the experimental results.

[0003] CN202220169U discloses a bacterial culture bottle for convenient sampling, with a culture bottle, the upper opening of the culture bottle has an outer seal and an inner seal sealed by an elastic ring, and two sampling ports closed by sealing plugs are provided at the bottom of the culture bottle. During use, bacteria culture is carried out according to routine inoculation. When sampling or adding other drugs or biological reagents is required in the middle or at the end of bacterial culture, the surface of a sealing plug in the sampling port is disinfected and sterilized, and then, a sterile syringe is used to extend into the bottle to suck out the culture solution containing bacteria and move it to the inspection site or to transfer the species. If biochemical reagents need to be added, another sealing plug can be selected, and after its surface is sterilized, biochemical drugs, reagents, etc. can be injected with a syringe. There is no need to carry out complicated opening disinfection and sterilization, so that the bacterial culture operation is simple and convenient. Although it is provided with a special sampling port at the bottom, it is still necessary to remove the lid and sterilize frequently. The device is designed with two lower ports, one for sampling and one for adding samples. However, the fixed port apertures do not accommodate the varying volume requirements of liquid sampling. For example, bacterial growth testing typically requires measuring in milliliters, while the addition of inducers and biological reagents typically requires measuring in microliters. This poses a risk of cross-contamination. Furthermore, the two ports are angled, making it difficult to use a pipette for sample addition and addition, as standard pipetting requires a perpendicular position to the liquid surface. Furthermore, the application does not specify the distance between the two ports and the bottom of the flask. This design approach should be tailored to suit different flask sizes, as the volume of bacterial solution directly impacts both sample removal and addition. For example, a 50ml Erlenmeyer flask typically contains 10ml of bacterial solution, while a 250ml Erlenmeyer flask typically contains 50ml. These volumes are typically relatively small, making this angled design difficult to use for sample removal and addition.

[0004] CN203474802U also discloses an anti-contamination bacterial culture bottle. Its main features include an inner and outer sealing membrane covering the bottle mouth, which are sealed to the bottle mouth via an elastic binding ring. Two or three sampling nozzles are provided on the bottle wall in the middle of the bottle body, the inner ends of the sampling nozzles communicate with the interior of the bottle body, and the outer ends of the sampling nozzles are provided with sealing plugs. Two or three conical protrusions protruding into the bottle body are provided at the junction of the bottle bottom and the bottle wall, and the conical protrusions are symmetrical along the center line of the bottle bottom. The bottle bottom is provided with supporting feet, the bottle wall is provided with scale lines, and the bottle body is provided with a thermometer. This culture bottle can ensure that the bacteria in the bottle obtain equal and sufficient nutrients during the bacterial culture process, and can prevent the bacteria in the bottle from secondary contamination during sampling, testing, and adding reagents, thereby ensuring the accuracy of the test results. However, the sampling port uses a sealed plug, which needs to be removed when in use, so that the part will be completely exposed to the air, increasing the risk of contamination; at the same time, since general incubators have thermometers, the thermometer and scale lines designed here are also redundant.

[0005] Although the above device solves the problem of increased contamination risk caused by adding samples during the culture process to a certain extent, it still has certain defects. Summary of the Invention

[0006] Purpose of the utility model: In order to solve the technical problems existing in the prior art, the utility model provides a bacterial culture device that is anti-pollution and convenient for adding and sampling, aiming to provide a new bacterial culture device with a simple structure, convenient operation and the ability to effectively prevent contamination.

[0007] To achieve the above-mentioned purpose, the present invention provides a contamination-proof bacterial culture device that is convenient for adding and taking samples, comprising a culture container body, a tray arranged at the opening of the culture container body, and a cover for closing the opening of the culture container body, wherein:

[0008] The lower surface of the tray is provided with a sample addition port, and a liquid absorbing material is provided in a groove other than the sample addition port;

[0009] The cover is an elastic sealing cover, and is provided with a plurality of elastic self-sealing holes with different apertures. When no external force is applied, the elastic self-sealing holes are in a closed state under the action of their own elastic material.

[0010] Wherein, the maximum pore size of the elastic self-enclosed hole is adapted to the aperture of the gun tip of the liquid-liquid transfer gun. Specifically, the maximum pore size of the elastic self-enclosed hole corresponds to the aperture of the gun tip of the liquid-liquid transfer gun of different ranges. Generally, when sampling, a liquid-liquid transfer gun is used. The gun tip of the unified standard configuration is divided into three colors. Generally, the blue gun tip is the maximum range 100uL-1ml, the yellow gun tip can measure 20uL-200uL, and the white gun tip can measure 0-10uL, 0-20uL. Therefore, the supporting design of aperture can be carried out according to the diameter of different gun tips. When using different ranges, it is inserted into different closed holes.

[0011] In a specific embodiment, the maximum aperture of the elastic self-sealing hole can be respectively corresponded to the ranges of three commonly used pipettes according to common needs, including a maximum range of 1 mL, 200 μL, and a maximum range of 20 μL.

[0012] Preferably, the sample adding port is arranged in a fan shape with the center point of the bottom surface of the tray as the vertex, and the area of ​​the sample adding port occupies one eighth of the entire bottom area of ​​the tray.

[0013] The tray is made of elastic rubber material, and its outer diameter is adapted to the inner diameter of the culture container body.

[0014] The tray is mainly set up to reserve a sample port for sample addition. At the same time, by setting a liquid-absorbing material in the groove, the sample liquid that may remain in the elastic self-sealing hole can be absorbed in time to avoid contamination in the next sample addition.

[0015] The cover is arranged closely on the upper side of the tray and is provided with a convex edge protruding from the opening of the culture container body, so as to facilitate taking and placing.

[0016] The elastic self-sealing holes are arranged radially in three rows, with each row containing three elastic self-sealing holes of different diameters. The elastic self-sealing holes on the same circumference have different diameters. This arrangement minimizes the number of elastic self-sealing holes while ensuring that different diameters are present in each radial direction, meeting diverse needs.

[0017] In order to further ensure the sealing of the culture device, a sealing cover is further provided above the lid.

[0018] In some embodiments, the culture container body is a culture bottle.

[0019] During use, the tray is first placed at the opening of the culture container body, and then the elastic sealing cover is placed above the tray so that the elastic self-sealing hole provided on the elastic sealing cover is located at the corresponding tray sample loading port. A suitable elastic self-sealing hole is selected for sample loading and sampling operations as needed. After the operation is completed, the elastic sealing cover is rotated to move the used elastic self-sealing hole to the groove of the tray. The residual liquid remaining in the used elastic self-sealing hole is absorbed by the liquid absorbing material at the groove, thereby avoiding the reuse of the used elastic self-sealing hole.

[0020] Beneficial effects: Compared with the prior art, the utility model has the following advantages:

[0021] (1) The design of the elastic self-sealing circular hole enables sample addition and sampling operations without opening the culture container, effectively preventing contamination by external bacteria and improving the accuracy and reliability of the experimental results;

[0022] (2) The provision of a tray with a sample loading port can avoid the risk of contamination caused by used elastic self-sealing holes;

[0023] (3) By optimizing the position of the elastic self-sealing hole, the same elastic sealing lid can be used in more applications to meet various experimental needs;

[0024] (4) The optional external closed lid further enhances the airtightness during the culture process and ensures the stability of the culture environment;

[0025] (5) The device has a simple structure and is easy to operate, which improves the operating efficiency and work comfort of the experimenters. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is a cross-sectional schematic diagram of a bacterial culture device;

[0027] Figure 2 Schematic diagram of a top view of a pallet;

[0028] Figure 3 This is a top view of the bacterial culture device. DETAILED DESCRIPTION

[0029] The present invention will be further described in detail below with reference to specific embodiments. The embodiments will help to understand the present invention, but the protection scope of the present invention is not limited to the following embodiments.

[0030] Example 1

[0031] like Figure 1-3 As shown, a contamination-proof bacterial culture device that is convenient for sample addition and sampling comprises a culture container body 1, a tray 4 disposed at the opening of the culture container body, and a lid 2 for closing the upper opening of the culture container body. The tray 4 is made of elastic rubber material, and the outer diameter of the tray 4 is adapted to the inner diameter of the culture container body, and the outer periphery thereof is tightly fitted with the inner diameter of the culture container body. The tray 4 is provided with a radial sector-shaped sample addition port 5 and a groove 6. A liquid absorbing material is disposed in the groove, which may be a liquid absorbing cotton sheet. The area of ​​the sector-shaped sample addition port occupies one eighth of the entire container body opening. Figure 2 As shown. An elastic sealing cover is provided above the tray, and the cover is provided with at least 9 elastic self-sealing holes of different apertures. The elastic self-sealing holes are in a closed state under the action of their own elastic material. The 9 elastic self-sealing holes include elastic self-sealing holes of 3 different aperture sizes. The three aperture sizes correspond to the diameters of the pipette tips with a maximum range of 1ml, a maximum range of 200uL, and a maximum range of 20uL, respectively. The 9 elastic self-sealing holes are arranged as shown. Figure 3 As shown, they are arranged radially in three rows, and each row is provided with three elastic self-sealing holes of different aperture sizes at intervals, and the apertures of the elastic self-sealing holes on the same circumference are different.

[0032] During use, the tray is first placed at the opening of the culture container body, and then the elastic sealing cover is placed above the tray so that the elastic self-sealing hole provided on the elastic sealing cover is located at the corresponding tray sample loading port. A suitable elastic self-sealing hole is selected for sample loading and sampling operations as needed. After the operation is completed, the elastic sealing cover is rotated to move the used elastic self-sealing hole to the groove of the tray. The liquid absorbing material at the groove absorbs the residual liquid remaining in the used elastic self-sealing hole, thereby avoiding the reuse of the used elastic self-sealing hole.

[0033] The present invention provides a contamination-resistant bacterial culture device that facilitates sample addition and removal. There are numerous methods and approaches for implementing this technical solution. The foregoing description is merely a preferred embodiment of the present invention. It should be noted that those skilled in the art may make various improvements and modifications without departing from the principles of the present invention, and such improvements and modifications are also within the scope of protection of the present invention. Any components not specified in this embodiment may be implemented using existing technologies.

Claims

1. A bacterial culture device that is pollution-proof and convenient for adding and sampling, characterized in that: It comprises a culture container body (1), a tray (4) arranged at an opening of the culture container body, and a cover (2) for closing the opening of the culture container body, wherein: The tray (4) is provided with a groove, in which a liquid absorbing material (6) is provided, and the tray is provided with a sample addition port (5); The cover (2) is an elastic sealing cover, and a plurality of elastic self-sealing holes with different apertures are provided on the cover (2). When no external force is applied, the elastic self-sealing holes are in a closed state under the action of their own elastic material.

2. The bacterial culture device according to claim 1, characterized in that The maximum aperture of the elastic self-sealing hole is adapted to the aperture of the pipette tip.

3. The bacterial culture device according to claim 1, characterized in that The sample addition port (5) is arranged in a fan shape with the center point of the bottom surface of the tray as the vertex, and the area of ​​the sample addition port (5) accounts for one eighth of the entire bottom area of ​​the tray.

4. The bacterial culture device according to claim 1, characterized in that The tray (4) is a tray made of elastic rubber material, and its outer diameter is adapted to the inner diameter of the opening of the culture container body (1).

5. The bacterial culture device according to claim 1, characterized in that The lid (2) is arranged closely above the tray (4) and is provided with a convex edge protruding from the opening of the culture container body.

6. The bacterial culture device according to claim 1, characterized in that The elastic self-sealing holes are arranged radially in three rows, and each row is provided with three elastic self-sealing holes of different aperture sizes at intervals, and the aperture sizes of the elastic self-sealing holes on the same circumference are different.

7. The bacterial culture device according to any one of claims 1 to 6, characterized in that: A sealing cover (3) is provided above the cover (2).

8. The bacterial culture device according to any one of claims 1 to 6, characterized in that: The culture container body (1) is a culture bottle.

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