Enzymolysis device for high-activity polypeptide production

Abstract

The utility model discloses an enzymolysis device for producing high-activity polypeptide, which relates to the technical field of polypeptide production, and comprises a bottom plate and a driving mechanism, two vertical plates are symmetrically arranged on the bottom plate, a top plate is arranged on the outer walls of the tops of the two vertical plates, a reaction tank is rotatably connected to the bottom plate, the top of the reaction tank is opened, and the driving mechanism is arranged on the bottom plate. The open end of the reaction tank is rotationally connected with the outer wall of the bottom of the top plate, a feeding port is formed in the top plate and communicated with the interior of the reaction tank, a gear ring is arranged on the outer wall of the reaction tank, a vertical rod is rotationally connected to the top plate, and a plurality of stirring blades are arranged on the outer wall, located in the reaction tank, of the vertical rod. In the use process, the stirring blades and the reaction tank can rotate in opposite directions, so that raw materials and enzymatic hydrolysate in the reaction tank can be well and uniformly mixed, the overall enzymolysis efficiency can be improved, and the practicability is high.

Description

Technical Field

[0001] The utility model relates to the technical field of polypeptide production, in particular to an enzymatic hydrolysis device for producing high-activity polypeptides. Background Art

[0002] Polypeptides are intermediate products of protein hydrolysis and have multiple biological functions, such as regulating cell growth, immune response, signal transduction, metabolism, etc. Enzymatic hydrolysis equipment is required in the production process of polypeptides.

[0003] A polypeptide production enzymolysis device with a search announcement number of CN214193281 U is disclosed, comprising a reaction tank, a heating plate provided at the bottom of the reaction tank, a vertical stirring shaft provided in the middle of the reaction tank, one end of the stirring shaft extending out of the reaction tank and connected to a motor transmission provided at the top of the reaction tank, stirring rods symmetrically provided on the surface of the stirring shaft, a water inlet pipe and an enzymolysis liquid pipe connected to the stirring shaft provided inside one of the stirring rods, a water outlet pipe and an enzymolysis liquid pipe connected to the stirring shaft provided inside the other stirring rod, and the orifices of the water inlet pipe, the water outlet pipe and the enzymolysis liquid pipe are all provided at the top of the stirring shaft, a plurality of liquid outlet heads provided on the surface of the stirring rod connected to the enzymolysis liquid pipe, a plurality of liquid outlet holes evenly distributed on the end of the liquid outlet head close to the enzymolysis liquid pipe, a lid provided on the other end of the liquid outlet head, one end of the lid connected to the liquid outlet head by a torsion spring. The utility model provides a plurality of liquid outlet heads on the surface of the stirring rod, so that the mixing between the enzymolysis liquid and the polypeptide is more uniform and faster.

[0004] In response to the aforementioned related technologies, the inventors believe that existing enzymatic hydrolysis devices for polypeptide production often only stir the hydrolyzate and polypeptide in the same direction, resulting in poor mixing uniformity between the hydrolyzate and polypeptide, which in turn affects the overall enzymatic hydrolysis efficiency. Therefore, we propose an enzymatic hydrolysis device for the production of high-activity polypeptides. Utility Model Content

[0005] In view of the above-mentioned problems existing in the existing enzymatic hydrolysis device for producing high-activity polypeptides, the present utility model is proposed.

[0006] Therefore, the purpose of the present invention is to provide an enzymatic hydrolysis device for the production of high-activity polypeptides, which solves the problem that the existing enzymatic hydrolysis devices for polypeptide production can only stir the enzymatic hydrolysis solution and the polypeptide in the same direction when stirring, resulting in poor mixing uniformity between the enzymatic hydrolysis solution and the polypeptide, which in turn affects the overall enzymatic hydrolysis efficiency.

[0007] In order to achieve the above purpose, the present invention provides the following technical solutions:

[0008] The top of the gear train is connected with the gear of the driven pulley, and the gear train is connected with the gear of the driven pulley respectively.

[0009] Preferably, a discharge port is provided at the bottom of the reaction tank, and the bottom of the reaction tank is a conical structure. A discharge pipe is provided on the bottom outer wall of the bottom plate, and the discharge pipe is rotatably connected to the discharge port.

[0010] Preferably, a support seat is provided on the bottom outer wall of the bottom plate, and the number of the support seats is four, and the four support seats are evenly distributed at the four corners of the bottom of the bottom plate.

[0011] Preferably, a plurality of paddles are arranged around the inner wall of the reaction tank.

[0012] Furthermore, the stirring blade is provided with a plurality of through holes.

[0013] Preferably, a heating plate is provided in the reaction tank, a battery is installed on the outer wall of the reaction tank, and the battery and the heating plate are connected by an electric wire.

[0014] In the above technical solution, the technical effects and advantages provided by the utility model are:

[0015] 1. During use, the utility model can realize that the stirring blade and the reaction tank rotate in opposite directions, so that the raw materials and enzymatic hydrolysis liquid inside the reaction tank can be well mixed, which is beneficial to improving the overall enzymatic hydrolysis efficiency and has strong practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 This is a schematic diagram of the three-dimensional structure of the reaction tank of the present invention;

[0019] Figure 3 This is a schematic diagram of the driving mechanism structure of the utility model;

[0020] Figure 4 This is a schematic diagram of the vertical rod and stirring blade structure of the utility model;

[0021] Figure 5 This is a schematic diagram of the cross-sectional structure of the reaction tank of the present invention.

[0022] Description of reference numerals:

[0023] 1. Bottom plate; 2. Reactor; 3. Vertical plate; 4. Top plate; 5. Feed port; 6. Drive mechanism; 7. Discharge pipe; 8. Support base; 9. Gear ring; 10. Paddle; 11. Battery; 12. Motor; 13. Rotating rod; 14. First pulley; 15. Second pulley; 16. Gear; 17. Vertical rod; 18. Stirring blade; 19. Heating plate. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0025] The embodiment of the utility model discloses an enzymatic hydrolysis device for producing high-activity polypeptides.

[0026] The utility model provides Figure 1-5 The enzymatic hydrolysis device for producing high-activity polypeptides shown in the figure includes a bottom plate 1 and a driving mechanism 6. Two vertical plates 3 are symmetrically arranged on the bottom plate 1. The top outer walls of the two vertical plates 3 are provided with a top plate 4. The bottom plate 1 is rotatably connected to a reaction tank 2. The top of the reaction tank 2 is open. The open end of the reaction tank 2 is rotatably connected to the bottom outer wall of the top plate 4. A feed port 5 is provided on the top plate 4. The feed port 5 is connected to the interior of the reaction tank 2. A gear ring 9 is provided on the outer wall of the reaction tank 2. A vertical rod 17 is rotatably connected to the top plate 4. The vertical rod 17 is located at the reaction tank 2. A plurality of stirring blades 18 are provided on the outer wall of the interior, and the driving mechanism 6 includes a motor 12, a rotating rod 13 and a second pulley 15. The motor 12 is mounted on the outer wall of the top plate 4 through a frame, and the rotating rod 13 is rotatably connected to the top plate 4. One end of the rotating rod 13 is fixedly connected to the output end of the motor 12. A first pulley 14 and a gear 16 are respectively provided on the rotating rod 13. The second pulley 15 is installed on the vertical rod 17. The first pulley 14 and the second pulley 15 are connected by a belt for transmission, and the gear 16 is meshed with the gear ring 9.

[0027] The utility model provides an enzymatic hydrolysis device for producing high-activity polypeptides. A discharge port is provided at the bottom of a reaction tank 2, and the bottom of the reaction tank 2 is a conical structure. A discharge pipe 7 is provided on the bottom outer wall of the bottom plate 1. The discharge pipe 7 is rotatably connected to the discharge port, which can facilitate the discharge of the enzymatically hydrolyzed polypeptides.

[0028] The utility model is an enzymatic hydrolysis device for producing high-activity polypeptides. A support seat 8 is provided on the bottom outer wall of the bottom plate 1. There are four support seats 8, which are evenly distributed at the four corners of the bottom of the bottom plate 1. They can provide stable support and improve the stability of the entire device during use. At the same time, they can also lift the bottom plate 1 to a certain height, thereby facilitating the collection of polypeptides discharged from the discharge pipe 7.

[0029] In the enzymatic hydrolysis device for producing high-activity polypeptides of the present invention, a plurality of paddles 10 are arranged around the inner wall of a reaction tank 2 to facilitate stirring of the material inside the reaction tank 2 .

[0030] The utility model provides an enzymatic hydrolysis device for producing high-activity polypeptides. A plurality of through holes are provided on the stirring blade 18 to improve the fluidity of the material and prevent the stirring blade 18 from being deformed or damaged due to an excessively large force area.

[0031] The utility model discloses an enzymatic hydrolysis device for producing high-activity polypeptides. A heating plate 19 is provided in a reaction tank 2, and a battery 11 is installed on the outer wall of the reaction tank 2. The battery 11 and the heating plate 19 are connected by an electric wire. The provision of the heating plate 19 can improve the enzymatic hydrolysis efficiency. The provision of the battery 11 on the outer wall of the reaction tank 2 can avoid the heating plate 19 being connected to an external power supply, thereby avoiding the phenomenon of the electric wire being entangled during the rotation of the reaction tank 2.

[0032] During use, the enzymolyzate and raw materials are transported to the interior of the reaction tank 2 through the feed port 5, and then the motor 12 is started to drive the rotating rod 13 to rotate. During the rotation process, the rotating rod 13 can drive the first pulley 14 and the gear 16 to rotate. Since the first pulley 14 and the second pulley 15 are connected by a belt transmission, the vertical rod 17 and the stirring blade 18 can be driven to rotate. Since the gear 16 is meshed with the gear ring 9, the reaction tank 2 and the stirring blade 18 can be driven to rotate in opposite directions, so that the raw materials and enzymolyzate inside the reaction tank 2 can be well mixed, which is beneficial to improving the enzymolysis efficiency. At the same time, the heating plate 19 can also be started to heat it, so as to further improve the enzymolysis efficiency.

[0033] The above description is merely illustrative of certain exemplary embodiments of the present invention. It goes without saying that those skilled in the art will be able to modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and description are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. An enzymatic hydrolysis device for producing high-activity polypeptides, comprising a base plate (1) and a driving mechanism (6), characterized in that: Two vertical plates (3) are symmetrically arranged on the bottom plate (1), and a top plate (4) is provided on the top outer walls of the two vertical plates (3). A reaction tank (2) is rotatably connected to the bottom plate (1), and the top of the reaction tank (2) is open. The open end of the reaction tank (2) is rotatably connected to the bottom outer wall of the top plate (4). A feed port (5) is provided on the top plate (4), and the feed port (5) is communicated with the interior of the reaction tank (2). A gear ring (9) is provided on the outer wall of the reaction tank (2). A vertical rod (17) is rotatably connected to the top plate (4), and a plurality of stirring blades (17) are provided on the outer wall of the vertical rod (17) located inside the reaction tank (2). 8), the driving mechanism (6) includes a motor (12), a rotating rod (13) and a second pulley (15), the motor (12) is mounted on the outer wall of the top plate (4) through a frame, the rotating rod (13) is rotatably connected to the top plate (4), one end of the rotating rod (13) is fixedly connected to the output end of the motor (12), the rotating rod (13) is respectively provided with a first pulley (14) and a gear (16), the second pulley (15) is mounted on a vertical rod (17), the first pulley (14) and the second pulley (15) are connected by a belt, and the gear (16) is meshed with the gear ring (9).

2. The enzymatic hydrolysis device for producing high-activity polypeptides according to claim 1, characterized in that: The bottom of the reaction tank (2) is provided with a discharge port, and the bottom of the reaction tank (2) is a conical structure. A discharge pipe (7) is provided on the bottom outer wall of the bottom plate (1), and the discharge pipe (7) is rotatably connected to the discharge port.

3. The enzymatic hydrolysis device for producing high-activity polypeptides according to claim 1, characterized in that: A support seat (8) is provided on the outer wall of the bottom of the base plate (1), and there are four support seats (8). The four support seats (8) are evenly distributed at the four corners of the bottom of the base plate (1).

4. The enzymatic hydrolysis device for producing high-activity polypeptides according to claim 1, characterized in that: A plurality of paddles (10) are arranged around the inner wall of the reaction tank (2).

5. The enzymatic hydrolysis device for producing high-activity polypeptides according to claim 1, characterized in that: The stirring blade (18) is provided with a plurality of through holes.

6. The enzymatic hydrolysis device for producing high-activity polypeptides according to claim 1, characterized in that: A heating plate (19) is provided in the reaction tank (2), a storage battery (11) is installed on the outer wall of the reaction tank (2), and the storage battery (11) and the heating plate (19) are connected via an electric wire.

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