Cell culture dish

Abstract

The utility model provides a cell culture dish, relates to cell culture dish field, including culture subassembly, culture subassembly includes casing, top cover, culture medium and connecting piece, casing and top cover are used for providing space for cell culture, culture medium is used for providing culture work space for cell culture, connecting piece is used for connecting top cover and casing, and the casing is used for connecting the top cover and the connecting piece. A temperature control assembly is mounted on the surface of the culture assembly and comprises a microcontroller, a heating sheet, a temperature sensor and a three-color indicator lamp; according to the cell culture device, by arranging the culture assembly, the effect of providing a culture space for cells can be achieved, so that the cells can be cultured, by arranging the temperature control assembly, the effect of providing constant temperature during cell culture can be achieved, and the temperature sensor is used for monitoring the temperature of a culture environment; the culture temperature can be controlled through the microcontroller and the heating sheet, so that the use function of the culture dish can be improved.

Description

Technical Field

[0001] The utility model belongs to the field of cell culture dishes, in particular to a cell culture dish. Background Art

[0002] Cell culture dishes are containers used to culture and propagate biological cells in vitro and are widely used in biomedical research, drug screening, and clinical diagnosis.

[0003] According to Chinese patent application number: 202020913628.1, a cell culture dish is disclosed, including an outer dish body, an inner dish body and a dish cover, and also includes: a first sealing plug, a plurality of return springs, a first permanent magnet, a knob, a rotating shaft, a second permanent magnet and a cell filter device;

[0004] Current technology has effectively solved the problem of inconvenient replacement of the inner substrate of cell culture dishes, which is easy to cause contamination. It has the advantage of convenient replacement of the inner substrate of the culture dish. However, the function of the culture dish is relatively single when in use, and it needs to be coordinated with an external constant temperature device to provide a culture environment for cells, thereby reducing the use effect of the culture dish.

[0005] In summary, the present invention provides a cell culture dish to solve the above problems. Utility Model Content

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0007] A cell culture dish includes a culture component, which includes a shell, a top cover, a culture medium and a connecting piece. The shell and the top cover are used to provide space for cell culture, the culture medium is used to provide a culture space for cell culture, and the connecting piece is used to connect the top cover and the shell. A temperature control component is installed on the surface of the culture component, and the temperature control component includes a microcontroller, a heating plate, a temperature sensor and a three-color indicator light. The temperature control component is used to provide a constant temperature environment for cell culture. An injection component is provided on the top of the top cover, and the injection component is used to add nutrient solution to cell culture.

[0008] Furthermore, in the present invention, the top cover is located on the top of the shell, and the culture medium is located in the inner cavity of the shell and is movably connected to the inner cavity of the shell.

[0009] Furthermore, in the present invention, the connecting piece includes a connecting column and an internal thread, the internal thread is opened on the inner wall of the top cover, the connecting column is fixed to the top of the shell, and the end of the connecting column away from the shell extends to the inner cavity of the top cover and is threadedly connected to the internal thread.

[0010] Furthermore, in the present invention, the microcontroller and the three-color indicator light are both mounted on the surface of the shell, the heating plate is mounted on the bottom of the shell cavity, and the temperature sensor is mounted on the top of the top cover cavity.

[0011] Furthermore, in the present invention, the output end of the temperature sensor is connected to the input end of the microcontroller, and the output end of the microcontroller is connected to the input ends of the heating plate and the three-color indicator light respectively.

[0012] Furthermore, in the present invention, the liquid injection assembly includes a liquid injection tube and a valve, one end of the liquid injection tube is connected to the top cover, and the valve is installed on the surface of the liquid injection tube.

[0013] Furthermore, in the present invention, the heating plate is a PET electric heating film, and the microcontroller adopts an ESP8266 single-chip microcomputer.

[0014] Beneficial effects: The utility model has the following beneficial effects:

[0015] The utility model provides a culture space for cells by arranging a culture component. The culture medium is used to store cells. The connecting piece is used to connect the shell and the top cover so that the shell and the top cover can provide a closed culture space for the cells, thereby enabling the cells to be cultured. The temperature control component is provided to provide a constant temperature during cell culture. The temperature sensor is used to monitor the temperature of the culture environment. The culture temperature can be controlled by the microcontroller and the heating plate, thereby improving the use function of the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the main structure of the utility model;

[0017] Figure 2 This is a schematic structural diagram of the utility model in a state where the shell and the culture medium are separated;

[0018] Figure 3 This is a schematic diagram of the top cover of the utility model when viewed from above;

[0019] Figure 4 It is a schematic diagram of the system flow of the utility model.

[0020] In the picture:

[0021] 1. Culture component; 11. Shell; 12. Top cover; 13. Culture medium; 14. Connector; 141. Connecting column; 142. Internal thread; 2. Temperature control component; 201. Microcontroller; 202. Heating plate; 203. Temperature sensor; 204. Three-color indicator light; 3. Liquid injection component; 301. Liquid injection tube; 302. Valve. DETAILED DESCRIPTION

[0022] In order to better understand the technical content of the present invention, specific embodiments are given and described as follows in conjunction with the accompanying drawings. Various aspects of the present invention are described in this disclosure with reference to the accompanying drawings, in which many illustrative embodiments are shown. The embodiments of the present disclosure are not necessarily defined to include all aspects of the present invention. It should be understood that the various concepts and embodiments introduced above, as well as those described in more detail below, can be implemented in any of many ways, because the concepts and embodiments disclosed in the present invention are not limited to any implementation method. In addition, some aspects disclosed in the present invention can be used alone or in any appropriate combination with other aspects disclosed in the present invention.

[0023] Example 1

[0024] like Figure 1-4 As shown, the first embodiment of the utility model provides a cell culture dish, including a culture component 1, the culture component 1 includes a shell 11, a top cover 12, a culture medium 13 and a connector 14, the shell 11 and the top cover 12 are used to provide space for cell culture, the culture medium 13 is used to provide culture space for cell culture, the connector 14 is used to connect the top cover 12 and the shell 11, a temperature control component 2 is installed on the surface of the culture component 1, the temperature control component 2 includes a microcontroller 201, a heating plate 202, a temperature sensor 203 and a three-color indicator light 204, the temperature control component 2 is used to provide a constant temperature environment for cell culture, and an injection component 3 is provided on the top of the top cover 12, and the injection component 3 is used to add nutrient solution for cell culture.

[0025] like Figure 1-4 As shown, the culture medium 13 is placed in the inner cavity of the shell 11, and then cells are injected into the inner cavity of the culture medium 13. The inner wall of the culture medium 13 is also coated with a surfactant poly-L-lysine to promote cell growth. Then the top cover 12 is closed, and the top cover 12 is sealed to the shell 11 and the culture medium 13 through the connector 14. The temperature sensor 203 monitors the temperature of the inner cavity of the culture medium 13 and sends the data to the microcontroller 201. The microcontroller 201 adjusts the heating temperature of the heating plate 202 according to the data. The three-color indicator light 204 is used for temperature indication. When the temperature of the inner cavity of the culture medium 13 is at a normal value, the three-color indicator light 204 lights up green. When the temperature of the inner cavity of the culture medium 13 is higher than the threshold, the three-color indicator light 204 lights up red. When the temperature of the inner cavity of the culture medium 13 is lower than the threshold, the three-color indicator light 204 lights up yellow, so that the staff can clearly understand the ambient temperature of the cell culture.

[0026] Example 2

[0027] Reference Figure 1-3 , which is the second embodiment of the present utility model, and this embodiment is based on the previous embodiment.

[0028] In this embodiment, the top cover 12 is located on the top of the shell 11 , and the culture medium 13 is located in the inner cavity of the shell 11 and is movably connected to the inner cavity of the shell 11 .

[0029] The connecting member 14 includes a connecting column 141 and an internal thread 142. The internal thread 142 is opened on the inner wall of the top cover 12. The connecting column 141 is fixed to the top of the shell 11. The end of the connecting column 141 away from the shell 11 extends to the inner cavity of the top cover 12 and is threadedly connected to the internal thread 142.

[0030] The liquid injection assembly 3 includes a liquid injection tube 301 and a valve 302 . One end of the liquid injection tube 301 is connected to the top cover 12 , and the valve 302 is installed on the surface of the liquid injection tube 301 .

[0031] like Figure 1-3 As shown, the top cover 12 is put on the top of the connecting column 141, and then the top cover 12 is rotated, the connecting column 141 is engaged with the internal thread 142, so that the bottom of the top cover 12 contacts the top of the shell 11, thereby sealing the culture medium 13 and providing a closed space for cell culture. The valve 302 is opened and the nutrient solution can be injected into the inner cavity of the culture medium 13 through the injection tube 301 to promote cell growth.

[0032] Example 3

[0033] Reference Figure 1-4 , which is the third embodiment of the present utility model, is based on the first two embodiments.

[0034] In this embodiment, the microcontroller 201 and the three-color indicator light 204 are both mounted on the surface of the housing 11 , the heating plate 202 is mounted on the bottom of the inner cavity of the housing 11 , and the temperature sensor 203 is mounted on the top of the inner cavity of the top cover 12 .

[0035] The output end of the temperature sensor 203 is connected to the input end of the microcontroller 201 , and the output end of the microcontroller 201 is connected to the input ends of the heating plate 202 and the three-color indicator light 204 respectively.

[0036] The heating plate 202 is a PET electric heating film, and the microcontroller 201 adopts an ESP8266 single chip microcomputer.

[0037] like Figure 1-4 As shown, the three-color indicator light 204 is used to indicate the temperature situation, the temperature sensor 203 can monitor the temperature of the inner cavity of the culture medium 13, and the microcontroller 201 is used to process the data monitored by the temperature sensor 203 and adjust the heating temperature of the heating plate 202, so that the temperature of the cell culture can be kept stable.

[0038] When in use, the culture medium 13 is first placed in the inner cavity of the shell 11, and then the cells are injected into the inner cavity of the culture medium 13. The top cover 12 is put on the top of the connecting column 141, and then the top cover 12 is rotated. The connecting column 141 is engaged with the internal thread 142, so that the bottom of the top cover 12 contacts the top of the shell 11, thereby sealing the culture medium 13 and providing a closed space for cell culture. The temperature sensor 203 can monitor the temperature of the inner cavity of the culture medium 13. The microcontroller 201 is used to process the data monitored by the temperature sensor 203 and adjust the heating plate. The heating temperature of 202 can keep the temperature of cell culture stable. The three-color indicator light 204 is used for temperature indication. When the temperature of the inner cavity of the culture medium 13 is at a normal value, the three-color indicator light 204 lights up green. When the temperature of the inner cavity of the culture medium 13 is higher than the threshold, the three-color indicator light 204 lights up red. When the temperature of the inner cavity of the culture medium 13 is lower than the threshold, the three-color indicator light 204 lights up yellow, so that the staff can clearly understand the ambient temperature of cell culture. By opening the valve 302, nutrient solution can be injected into the inner cavity of the culture medium 13 through the injection tube 301 to promote cell growth, thereby improving the use function of the culture dish.

[0039] The standard parts used in this application document can all be purchased from the market, and can be customized according to the description in the specification and drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the existing technology. The machinery, parts and equipment all adopt conventional models in the existing technology. The control method is automatic control through a controller. The control circuit of the controller can be implemented by simple programming by technicians in this field, which is common knowledge in this field. In addition, this application is mainly used to protect mechanical devices, so this application no longer explains the control method and circuit connection in detail.

[0040] While the present invention has been described above with reference to preferred embodiments, this is not intended to limit the present invention. Persons skilled in the art will readily appreciate that various modifications and variations may be made without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention shall be determined by the claims.

Claims

1. A cell culture dish comprising a culture component (1), characterized in that: The culture component (1) comprises a shell (11), a top cover (12), a culture medium (13) and a connector (14); the shell (11) and the top cover (12) are used to provide space for cell culture; the culture medium (13) is used to provide a culture space for cell culture; the connector (14) is used to connect the top cover (12) and the shell (11); a temperature control component (2) is installed on the surface of the culture component (1); the temperature control component (2) comprises a microcontroller (201), a heating plate (202), a temperature sensor (203) and a three-color indicator light (204); the temperature control component (2) is used to provide a constant temperature environment for cell culture; a liquid injection component (3) is provided on the top of the top cover (12); the liquid injection component (3) is used to add nutrient solution for cell culture.

2. The cell culture dish according to claim 1, wherein: The top cover (12) is located on the top of the shell (11), and the culture medium (13) is located in the inner cavity of the shell (11) and is movably connected to the inner cavity of the shell (11).

3. The cell culture dish according to claim 1, wherein: The connecting member (14) includes a connecting column (141) and an internal thread (142), wherein the internal thread (142) is formed on the inner wall of the top cover (12), the connecting column (141) is fixed to the top of the shell (11), and one end of the connecting column (141) away from the shell (11) extends to the inner cavity of the top cover (12) and is threadedly connected to the internal thread (142).

4. The cell culture dish according to claim 1, wherein: The microcontroller (201) and the three-color indicator light (204) are both mounted on the surface of the housing (11), the heating plate (202) is mounted on the bottom of the inner cavity of the housing (11), and the temperature sensor (203) is mounted on the top of the inner cavity of the top cover (12).

5. The cell culture dish according to claim 1, wherein: The output end of the temperature sensor (203) is connected to the input end of the microcontroller (201), and the output end of the microcontroller (201) is respectively connected to the input ends of the heating plate (202) and the three-color indicator light (204).

6. The cell culture dish according to claim 1, wherein: The liquid injection assembly (3) comprises a liquid injection pipe (301) and a valve (302). One end of the liquid injection pipe (301) is connected to the top cover (12), and the valve (302) is installed on the surface of the liquid injection pipe (301).

7. The cell culture dish according to claim 1, wherein: The heating plate (202) is a PET electric heating film, and the microcontroller (201) adopts an ESP8266 single chip microcomputer.

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