L-citrulline continuous conversion tank

Abstract

The utility model relates to the technical field of bioconversion, in particular to an L-citrulline continuous conversion tank which comprises a tank body, a rotating shaft is arranged in the tank body, a stirring paddle is arranged at the bottom of the rotating shaft, the top of the rotating shaft penetrates out of the tank body to be connected with a first motor, and a hollow lead screw is sleeved outside the rotating shaft. After catalytic conversion is completed, the ultrafiltration membrane sieve plate moves downwards to carry out filter pressing, immediate separation of citrulline clear liquid and whole-cell enzyme is achieved, the citrulline clear liquid is discharged from the discharging port, the whole-cell enzyme is gathered at the bottom of the tank, and after the ultrafiltration membrane sieve plate is sprayed and cleaned, the whole-cell enzyme can be separated from the whole-cell enzyme. The arginine solution is supplemented for secondary conversion, secondary utilization and even repeated utilization of the whole-cell enzyme are realized, external filtering equipment is not needed, the use cost is reduced, and the filtering efficiency is improved through immediate filtering; the method is simple and convenient to use, saves enzyme resources and has higher conversion efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of biological transformation, in particular to an L-citrulline continuous transformation tank. Background Art

[0002] Citrulline is a basic amino acid that participates in the human urea cycle. As an important functional amino acid, L-citrulline exhibits antioxidant, vasodilator, arteriosclerosis inhibitory properties, male sexual function improvement, and blood pressure and blood sugar regulation. It is often added to functional foods as a nutritional supplement. The industrial production of L-citrulline uses L-arginine as a substrate. Whole-cell catalysis is performed using bacterial cells containing arginine deiminase, obtained through fermentation. The enzyme then catalyzes the conversion of L-citrulline into L-citrulline in a single step.

[0003] Traditional L-citrulline conversion mostly uses whole-cell arginine deiminase for a single catalytic process. After the catalytic process is completed, the feed liquid is discharged and filtered through an external filtration device to remove the whole-cell enzyme and impurities before L-citrulline is separated and purified. Since whole-cell catalysis maintains the enzyme's original physiological environment and state, it improves the enzyme's stress resistance and ensures the enzyme's stability and adaptability, thus enabling repeated batch conversions. Direct discharge of the feed liquid after a single catalytic process often results in enzyme waste, increases production equipment costs, and results in low conversion efficiency. Utility Model Content

[0004] In view of the above-mentioned defects, the purpose of the present invention is to provide an L-citrulline continuous conversion tank to solve the problems of increased production costs due to external filtering equipment and low conversion efficiency.

[0005] In order to achieve the above-mentioned object, the utility model provides an L-citrulline continuous conversion tank, comprising a tank body, a rotating shaft is provided in the tank body, a stirring paddle is provided at the bottom of the rotating shaft, and the top of the rotating shaft passes through the tank body and is connected to a first motor, a hollow screw rod is sleeved on the outside of the rotating shaft, a rotatable ultrafiltration membrane sieve plate is provided on the hollow screw rod, the top of the hollow screw rod passes through the tank body and is connected to a driven gear, the driven gear meshes with a driving gear, and the driving gear is connected to a second motor.

[0006] As a preferred technical solution, the ultrafiltration membrane sieve plate is circular, and a through hole is provided at the center of the ultrafiltration membrane sieve plate for socketing the rotating shaft; the ultrafiltration membrane sieve plate includes an outer circular frame and an inner circular frame, and two layers of upper and lower mesh brackets are provided between the outer circular frame and the inner circular frame, and an ultrafiltration membrane layer is provided between the upper and lower mesh brackets.

[0007] As a preferred technical solution, the ultrafiltration membrane layer is a ceramic membrane with a thickness of 200 to 350 nm.

[0008] As a preferred technical solution, a plurality of reinforcing ribs are provided at equal angles between the outer circular frame and the inner circular frame.

[0009] As a preferred technical solution, a soft rubber ring is provided on the outside of the outer circular frame.

[0010] As a preferred technical solution, a spray device is provided above the ultrafiltration membrane sieve plate.

[0011] As a preferred technical solution, a feed port is provided on the upper side wall of the tank body, and a drain valve is provided on the bottom of the tank body; a jacket is provided on the outside of the tank body, a jacket medium inlet is provided on the side wall below the jacket, and a jacket medium outlet is provided on the side wall above the jacket; the heights of the feed port and the jacket medium outlet are lower than the highest position of the ultrafiltration membrane sieve plate in the tank body.

[0012] As a preferred technical solution, the feed port is detachable.

[0013] As a preferred technical solution, a plurality of discharge ports are provided on the side wall of the tank body and on the opposite side of the feed port.

[0014] As a preferred technical solution, an electrode socket is provided at the bottom of the tank body, and a pH / temperature electrode is inserted into the electrode socket.

[0015] The L-citrulline continuous conversion tank provided by the utility model has the advantages that after the catalytic conversion is completed, the ultrafiltration membrane sieve plate moves downward for pressure filtration to achieve instant separation of the citrulline clear liquid and the whole-cell enzyme, the citrulline clear liquid is discharged from the discharge port, and the whole-cell enzyme is accumulated at the bottom of the tank, after the ultrafiltration membrane sieve plate is sprayed and cleaned, arginine solution is added for secondary conversion, thereby achieving secondary or even multiple utilization of the whole-cell enzyme, and no external filtering equipment is required, thus reducing the use cost, and instant filtration improves the filtration efficiency; the utility model is simple and convenient to use, saves enzyme resources, and has higher conversion efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic structural diagram of the L-citrulline continuous conversion tank of the utility model;

[0017] Figure 2 Schematic diagram of the structure of the ultrafiltration membrane sieve plate;

[0018] In the picture:

[0019] 1-tank body, 2-rotating shaft, 3-stirring paddle, 4-first motor, 5-second motor, 6-driving gear, 7-driven gear, 8-spraying device, 9-hollow screw, 10-feed port, 11-drain valve, 12-electrode jack, 13-discharge port, 14-jacketed medium inlet, 15-jacketed medium outlet, 16-jacketed drain port, 17-ultrafiltration membrane sieve plate, 171-outer circular frame, 172-mesh bracket, 173-ultrafiltration membrane layer, 174-reinforcement rib, 175-through hole, 176-inner circular frame, 177-soft rubber ring. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0021] Example 1:

[0022] See also Figure 1 The utility model provides an L-citrulline continuous conversion tank, comprising a tank body 1, wherein the tank body 1 is used for containing a base material arginine solution and whole-cell enzyme.

[0023] A rotating shaft 2 is provided in the tank body 1 , a stirring paddle 3 is provided at the bottom of the rotating shaft 2 , and a top of the rotating shaft 2 passes through the tank body 1 and is connected to a first motor 4 .

[0024] The outer portion of the rotating shaft 2 is provided with a hollow screw 9, on which a rotatable ultrafiltration membrane sieve plate 17 is provided. The top of the hollow screw 9 passes through the tank body 1 and is connected to a driven gear 7. The driven gear 7 engages with a driving gear 6, and the driving gear 6 is connected to a second motor 5. The second motor 5 drives the driving gear 6 and the driven gear 7 to rotate, thereby driving the hollow screw 9 to rotate. The rotation of the hollow screw 9 drives the ultrafiltration membrane sieve plate 17 to move up and down.

[0025] See also Figure 2 The shape of the ultrafiltration membrane sieve plate 17 is adapted to the cross-section of the tank body 1. In this embodiment, a barrel-shaped tank body is used as an example for description, so the ultrafiltration membrane sieve plate 17 is circular. A through hole 175 is provided at the center of the ultrafiltration membrane sieve plate 17 for sleeve-mounting the rotating shaft 2. The ultrafiltration membrane sieve plate 17 includes an outer circular frame 171 and an inner circular frame 176. An upper and lower layer of mesh supports 172 are provided between the outer and inner circular frames 171 and 176. An ultrafiltration membrane layer 173 is provided between the upper and lower layers of mesh supports 172. The ultrafiltration membrane layer 173 is a ceramic membrane with a diameter of 200 to 350 nm.

[0026] To enhance the strength of the ultrafiltration membrane sieve plate 17 during up and down movement and prevent deformation, multiple reinforcing ribs 174 are provided at equal angles between the outer circular frame 171 and the inner circular frame 176. To prevent whole-cell enzymes from entering the upper portion of the ultrafiltration membrane sieve plate 17 through the gap between the outer circular frame 171 and the inner wall of the tank 1, a soft rubber ring 177 is provided on the outside of the outer circular frame 171.

[0027] See also Figure 1 A spray device 8 is provided above the ultrafiltration membrane sieve plate 17. All spray devices 8 can adopt existing technologies, so they are not described in detail.

[0028] The upper side wall of the tank body 1 is provided with a detachable feed port 10, which can be set to a larger aperture to improve feeding efficiency. The feed port 10 can be set to a trumpet shape to facilitate external soft water pipe or elbow to flush the bottom of the ultrafiltration membrane sieve plate 17.

[0029] A drain valve 11 is provided at the bottom of the tank 1. Multiple discharge ports 13 are provided on the sidewall of the tank 1, opposite the feed port 10. The provision of multiple discharge ports 13 allows for the timely discharge of filtered material during filtration, improving discharge efficiency while shortening the time required for secondary or multiple conversions.

[0030] The bottom of the tank body 1 is also provided with an electrode socket 12. The electrode socket 12 can be inserted into the pH / temperature electrode to monitor the pH and temperature of the conversion process, so that the user can control and achieve the optimal conversion conditions.

[0031] The tank body 1 is provided with a jacket on the outside. A jacket medium inlet 14 is provided on the sidewall below the jacket, and a jacket medium outlet 15 is provided on the sidewall above the jacket. The jacket medium inlet 14 and jacket medium outlet 15 are located on opposite sides. The medium in the jacket is water, but other media can be selected as needed. By circulating water of a certain temperature into the jacket, the temperature can be kept within a suitable range for conversion, thereby accelerating the enzymatic reaction. A jacket drain outlet 16 is also provided at the bottom of the jacket.

[0032] The tank body 1 is fixed by a support frame. The material of the tank body 1 can be corrosion-resistant stainless steel to widely withstand acid and alkali solutions.

[0033] The heights of the feed port 10 and the jacket medium outlet 15 are slightly lower than the highest position of the ultrafiltration membrane sieve plate 17 in the tank body 1. The jacket medium inlet 14 and the lowest discharge port 13 are opposite and at the same height.

[0034] The working principle of the present invention is as follows: the substrate arginine solution and whole-cell enzyme are pumped into the tank body 1 through the feed port 10. The conversion temperature is maintained by the jacket, and stirring is performed to promote the conversion reaction. After the conversion is completed in the tank body 1 and the product level reaches the desired value, the first motor 4 is turned off and stirring is stopped. The second motor 5 is activated, ultimately driving the ultrafiltration membrane sieve plate 17 downward along the hollow screw 9. The clear liquid passes through the ultrafiltration membrane sieve plate 17 and enters the upper part, while the enzyme is trapped below the ultrafiltration membrane sieve plate 17. After the ultrafiltration membrane sieve plate 17 is filtered, the corresponding discharge port 13 is opened to discharge the clear liquid. The ultrafiltration membrane sieve plate 17 rises to the highest position at the top of the tank body 1, and the spray device 8 is activated to spray and clean the ultrafiltration membrane sieve plate 17. After cleaning is completed, the substrate arginine solution is replenished through the feed port 10, and secondary and multiple conversions are performed. After multiple conversions, the whole-cell enzyme activity decreases and can be discharged through the drain valve 11, while the tank body 1 is cleaned at the same time. The device is simple and convenient to use, has higher conversion efficiency, and can save enzyme resources.

[0035] The L-citrulline continuous conversion tank provided by the utility model has the advantages that after the catalytic conversion is completed, the ultrafiltration membrane sieve plate moves downward for pressure filtration to achieve instant separation of the citrulline clear liquid and the whole-cell enzyme, the citrulline clear liquid is discharged from the discharge port, and the whole-cell enzyme is accumulated at the bottom of the tank, after the ultrafiltration membrane sieve plate is sprayed and cleaned, arginine solution is added for secondary conversion, thereby achieving secondary or even multiple utilization of the whole-cell enzyme, and no external filtering equipment is required, thus reducing the use cost, and instant filtration improves the filtration efficiency; the utility model is simple and convenient to use, saves enzyme resources, and has higher conversion efficiency.

[0036] Of course, the present invention may have many other embodiments. Without departing from the spirit and essence of the present invention, technicians familiar with the field can make various corresponding changes and modifications based on the present invention, but these corresponding changes and modifications should all fall within the scope of protection of the claims attached to the present invention.

Claims

1. An L-citrulline continuous conversion tank, comprising a tank body (1), a rotating shaft (2) disposed in the tank body (1), a stirring paddle (3) disposed at the bottom of the rotating shaft (2), and a first motor (4) connected to the top of the rotating shaft (2) passing through the tank body (1), wherein: The outer portion of the rotating shaft (2) is provided with a hollow screw rod (9), a rotatable ultrafiltration membrane sieve plate (17) is provided on the hollow screw rod (9), the top of the hollow screw rod (9) passes through the tank body (1) and is connected to a driven gear (7), the driven gear (7) meshes with a driving gear (6), and the driving gear (6) is connected to a second motor (5).

2. A L-citrulline continuous conversion tank according to claim 1, characterized in that, The ultrafiltration membrane sieve plate (17) is circular, and a through hole (175) is provided at the center of the ultrafiltration membrane sieve plate (17) for sleeve-connecting the rotating shaft (2); the ultrafiltration membrane sieve plate (17) comprises an outer circular frame (171) and an inner circular frame (176), and an upper and lower layer of mesh supports (172) are provided between the outer circular frame (171) and the inner circular frame (176), and an ultrafiltration membrane layer (173) is provided between the upper and lower layers of mesh supports (172).

3. A L-citrulline continuous conversion tank according to claim 2, characterized in that, The ultrafiltration membrane layer (173) is a ceramic membrane with a thickness of 200 to 350 nm.

4. A L-citrulline continuous conversion tank according to claim 2, characterized in that, A plurality of reinforcing ribs (174) are provided at equal angles between the outer circular frame (171) and the inner circular frame (176).

5. An L-citrulline continuous conversion tank according to any one of claims 2 to 4, characterized in that: A soft rubber ring (177) is provided on the outside of the outer circular frame (171).

6. An L-citrulline continuous conversion tank according to claim 1, characterized in that, A spray device (8) is provided above the ultrafiltration membrane sieve plate (17).

7. An L-citrulline continuous conversion tank according to claim 6, characterized in that, The upper side wall of the tank body (1) is provided with a feed port (10), and the bottom of the tank body (1) is provided with a drain valve (11); a jacket is provided on the outside of the tank body (1), a jacket medium inlet (14) is provided on the side wall below the jacket, and a jacket medium outlet (15) is provided on the side wall above the jacket; the heights of the feed port (10) and the jacket medium outlet (15) are lower than the highest position of the ultrafiltration membrane sieve plate (17) located in the tank body (1).

8. An L-citrulline continuous conversion tank according to claim 7, characterized in that, The feed port (10) is detachably arranged.

9. The L-citrulline continuous conversion tank according to claim 7, wherein: A plurality of discharge ports (13) are provided on the side wall of the tank body (1) and on the side opposite to the feed port (10).

10. The L-citrulline continuous conversion tank according to claim 7, characterized in that: The bottom of the tank body (1) is provided with an electrode socket (12), and a pH / temperature electrode is plugged into the electrode socket (12).

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