Leaf in-vitro fresh-keeping preservation device

Abstract

The utility model discloses a leaf in-vitro fresh-keeping storage device which comprises a box body, a box cover and a movable partition plate, the movable partition plate is used for dividing the inner space of the box body into compartments, and the compartments are used for storing in-vitro leaves; an interlayer is arranged between the side wall of the box body and / or the movable partition plate and is communicated with the outside through a channel; the fresh-keeping storage device further comprises water absorption paper or water absorption sponge, and the water absorption paper and / or the water absorption sponge are / is located in the interlayer and used for storing the nutrient solution. By means of the fresh-keeping storage device, the humidity in the box body can be guaranteed, it is guaranteed that the interior of the box body is in a low-temperature environment, meanwhile, a nutrient solution can be continuously supplied to the in-vitro leaves, and the requirements that the in-vitro leaves are transported back to a laboratory from the field to be refrigerated and can be preserved for a long time in the refrigeration process are met.

Description

Technical Field

[0001] The present application relates to the field of cold chain preservation technology, and in particular to a device for preserving and storing detached leaves. Background Art

[0002] Orchids are one of my country's ten traditional famous flowers, with a cultivation history of over 2,000 years. Orchids are characterized by their unique leaves, flowers, and fragrance, each possessing four qualities: pure air, pure color, pure spirit, and pure rhythm. They are characterized by purity and elegance. China is a major distribution center for orchids, with 31 known species, representing over half of the world's orchids, primarily terrestrial orchids. Among these orchids, the Chinese orchid is a highly ornamental terrestrial orchid with a rich variety of over a thousand species, including the spring orchid, the cymbidium orchid, the jian orchid, the black orchid, and the cold orchid.

[0003] During the long-term artificial selection and cultivation process of Huilan, a group of new germplasms with stable leaf color variations were obtained, that is, the leaves were inlaid with golden or vermilion stripes or patches in different positions, which are called "leaf art"; the ornamental and commercial value of leaf art varieties has increased exponentially, becoming an important goal of breeding.

[0004] Due to the high commodity price and the instability of genetic variation, destructive sampling strategies and preservation measures have become restrictive steps in its scientific research.

[0005] When collecting samples, some preservation methods, such as vacuum packaging, sealed plastic bags, cardboard boxes, and direct exposure, often cause samples to oxidize or lose water before they are even brought back to the laboratory. Storage in the refrigerator can also lead to drying and wilting due to water loss, or rotting due to airtightness. These methods are not suitable for long-term storage and can easily damage the physical and chemical properties of leaf samples. Freezing with dry ice and liquid nitrogen can effectively preserve the physical and chemical properties of leaves, but this can damage their morphological characteristics and is unsuitable for orchid samples, where leaf morphology is crucial.

[0006] Therefore, it is urgent to find more effective measures to transport detached leaves from the wild back to the laboratory for refrigeration and keep them fresh for a longer period of time in refrigeration. Utility Model Content

[0007] The embodiments of the present application provide a device for preserving and storing detached leaves, which can meet the needs of transporting detached leaves from the field to a laboratory for refrigeration and preserving them for a long time in the refrigeration.

[0008] The present application discloses a device for preserving and preserving isolated leaves, comprising:

[0009] Box;

[0010] A box cover, the box cover is matched with the box body opening;

[0011] a first moisturizing device and a second moisturizing device, wherein the first moisturizing device and the second moisturizing device are absorbent paper or absorbent sponge for storing nutrient solution;

[0012] When in use, the humidity of the first moisturizing device is greater than that of the second moisturizing device. The first moisturizing device is used to contact the petiole of the detached leaf, and the second moisturizing device is used to contact the leaf of the detached leaf.

[0013] By adopting the detached leaf preservation device disclosed in the present application, the humidity inside the box can be guaranteed, and at the same time, the detached leaves can be continuously supplied with nutrient solution. By utilizing the humidity difference between the first moisturizing device and the second moisturizing device, the petioles of the detached leaves can absorb the nutrient solution, thereby ensuring the activity of the detached leaves. It can also ensure that the inside of the box is in a low-temperature environment, meeting the needs of transporting the detached leaves from the field back to the laboratory for refrigeration and preserving them for a long time in the refrigeration.

[0014] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become obvious from the description below, or will be learned through practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The above and / or additional aspects and advantages of the present application will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0016] Figure 1 This is a schematic diagram of the three-dimensional structure of the in vitro leaf preservation device in an embodiment of the present application;

[0017] Figure 2 This is a schematic diagram of the exploded structure of the first separator in use in an embodiment of the present application;

[0018] Figure 3 This is a schematic diagram of the exploded structure of the second separator in use in an embodiment of the present application;

[0019] Figure 4 This is a schematic diagram of the internal structure of the box in the embodiment of the present application;

[0020] Figure 5 Schematic diagram of the exploded structure of the first separator in the embodiment of the present application;

[0021] Figure 6 Schematic diagram of the exploded structure of the second separator in the embodiment of the present application;

[0022] Figure 7 It is a schematic structural diagram of the box cover in an embodiment of the present application.

[0023] Description of main component symbols:

[0024] Box body 100, interlayer 110, absorbent paper / sponge 120, channel 130, chute 140, top protrusion 141, bottom protrusion 142;

[0025] Box cover - 200, peripheral protrusion - 210;

[0026] Movable partition 300, interlayer 310, absorbent paper / absorbent sponge 320, channel 330, first partition 340, first channel 341, first fixing rib 342, second channel 343, second fixing rib 344, second partition 350, label attachment area 360. DETAILED DESCRIPTION

[0027] The embodiments of the present application are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application and are not to be construed as limiting the present application.

[0028] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more features. In the description of the present application, "multiple" means two or more, unless otherwise clearly and specifically defined.

[0029] In the description of this application, it should be noted that, unless otherwise expressly specified or limited, the terms "installed," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections, electrical connections, or mutual communication; they can refer to direct connections or indirect connections through an intermediate medium; they can refer to internal communication between two components or the interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in this application based on specific circumstances.

[0030] In this application, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Moreover, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0031] The disclosure below provides many different embodiments or examples for realizing different structures of the present application. In order to simplify the disclosure of the present application, the components and settings of specific examples are described below. Of course, they are merely examples and are not intended to limit the present application. In addition, the present application may repeat reference numbers and / or reference letters in different examples, and such repetition is for the purpose of simplicity and clarity, and does not itself indicate the relationship between the various embodiments and / or settings discussed. In addition, the present application provides examples of various specific processes and materials, but those of ordinary skill in the art will appreciate the application of other processes and / or the use of other materials.

[0032] The embodiment of the present application provides a device for preserving and preserving detached leaves, such as Figure 1-Figure 7 As shown, it includes a box body 100; a box cover 200 that matches the opening of the box body 100; a first moisturizing device and a second moisturizing device, wherein the first moisturizing device and the second moisturizing device are absorbent paper or absorbent sponge 120 (320) for preserving nutrient solution; when in use, the humidity of the first moisturizing device is greater than that of the second moisturizing device, the first moisturizing device is used to contact the petiole of the detached leaf, the second moisturizing device is used to contact the leaf of the detached leaf, the second moisturizing device is used to ensure that the leaf of the detached leaf has a certain humidity, and the humidity of the first moisturizing device is higher and is in contact with the petiole. During the leaf preservation process, the nutrient solution in the first moisturizing device can penetrate into the petiole and then be supplied to the detached leaf to ensure the freshness of the detached leaf.

[0033] In some embodiments, a movable partition 300 is further included. The movable partition 300 is located inside the box 100 and is used to separate the internal space of the box 100 to form a compartment, and the compartment is used to store the separated leaves. An interlayer 110 (310) is provided between the side wall of the box 100 and / or the movable partition 300. The interlayer 110 (310) is connected to the outside through a channel 130 (330); and a first moisturizing device or a second moisturizing device is provided inside the interlayer 110 (310).

[0034] The movable partition 300 can separate the space inside the box 100 as needed. For example, when collecting samples of a larger space and samples of a smaller space at the same time, the space can be reasonably separated. An interlayer 110 (310) is set in the middle of the box 100 and / or the partition, and an absorbent paper 120 (320) or an absorbent sponge 120 (320) for storing nutrient solution is set in the interlayer 110 (310). The nutrient solution stored in the absorbent paper or absorbent sponge 120 (320) can be transported to the outside of the interlayer 110 (310) through the channel 130 (330), and then supplied to the detached leaves. The absorbent paper or absorbent sponge 120 (320) can ensure the humidity inside the box 100 and prevent the leaves from drying up. If a bactericidal and antiseptic component is added to the nutrient solution, it can further prevent the leaves from rotting and further optimize the preservation effect. During use, the absorbent paper or absorbent sponge 120 (320) with the nutrient solution absorbed can also be frozen and then placed in the interlayer 110 (310) after freezing, which can ensure that the interior of the box 100 is in a low-temperature environment, so that the fresh-keeping storage device can maintain a low temperature even when it leaves the refrigerator, thereby ensuring the fresh-keeping effect.

[0035] For example, absorbent paper can be placed in the interlayer 310 of the movable partition 300, and an absorbent sponge can be placed in the interlayer 110 of the sidewall of the box 100. The humidity of the absorbent paper should be lower than that of the sponge. When placing leaves, the leaves are fixed to the upper side of the partition 300, so that they come into contact with the absorbent paper through the channel 330. After the partition 300 is placed in the box 100, the petiole of the leaf passes through the channel 130 of the interlayer 110 of the sidewall of the box 100, coming into contact with the absorbent sponge. The nutrient solution in the absorbent sponge then penetrates the petiole, ensuring the activity of the leaf.

[0036] The box body 100 and the box cover 200 are preferably made of polyethylene, which has a heat-insulating effect and a strong anti-deformation ability, thereby further protecting the separated leaves stored inside the fresh-keeping device.

[0037] Preferably, when in use, the humidity of the first moisturizing device is 60%-70%, and the humidity of the second moisturizing device is 50%-60%. The humidity within this range can not only achieve good heat preservation and moisturizing effects, but also prevent the leaves from being soaked by excessive humidity, thereby achieving a better preservation effect.

[0038] In some embodiments, the box body and the box cover are cold storage media, and / or the interlayer is provided with a cold storage medium, and / or the first moisturizing device and the second moisturizing device are cold storage media. By providing a cold storage medium, the box body can achieve the effect of keeping cold. Before use, the box body and the box cover can be frozen first, so that the cold storage medium can store cold, and can be taken out and used directly when in use. The first moisturizing device and the second moisturizing device can also absorb low-temperature nutrient solution to achieve the purpose of cold storage. It is also possible to add other cold storage media that have already stored cold to the interlayer, or add cold storage media that have not yet stored cold and put them into the refrigerator together with the box body and the box cover to store cold for next use.

[0039] In certain embodiments, as Figure 2 、 Figure 5 and Figure 6 As shown, the side wall of the box body 100 and / or the top of the movable partition 300 are provided with a pull-out opening with the same outline as the interlayer 110 (310), and the absorbent paper or absorbent sponge 120 (320) can be pulled out or put into the interlayer 110 (310) through the pull-out opening. By providing the pull-out opening, the taking and putting of the absorbent paper or absorbent sponge 120 (320) can be facilitated. If the outline of the absorbent paper or absorbent sponge 120 (320) is the same as the cross-section of the interlayer 110 (310), the absorbent paper or absorbent sponge 120 (320) will not be greatly deformed when it is put in, thereby avoiding deformation and squeezing of the absorbent paper or absorbent sponge 120 (320), thereby avoiding the loss of water or nutrient solution caused by squeezing.

[0040] In certain embodiments, as Figure 7 As shown, a peripheral protrusion 210 with the same contour as the box body 100 is provided under the box cover 200. The peripheral protrusion 210 is sealed with the box opening of the box body 100 and abuts against the upper part of the movable partition 300. The peripheral protrusion 210 can maintain a seal with the box opening of the box body 100 to prevent moisture evaporation. In addition, the peripheral protrusion 210 can also abut against the upper part of the movable partition 300, thereby fixing the movable partition 300 and preventing the movable partition 300 from loosening and squeezing the detached blades.

[0041] In some embodiments, pairs of slide grooves 140 are provided on the long and / or short sides of the inner wall of the box body 100, and the movable partition 300 is movably disposed within the slide grooves 140. The slide grooves 140 can, on the one hand, guide the movable partition 300 when it is installed into the box body 100, thereby facilitating the installation of the movable partition 300; on the other hand, after the movable partition 300 is installed, the slide grooves 140 can also provide a fixed position for the movable partition 300. In some embodiments, the slide grooves 140 cooperate with the peripheral protrusions 210 below the box cover 200 and the bottom of the box body 100 to fully position the movable partition 300, so that the movable partition 300 no longer moves.

[0042] In certain embodiments, as Figure 4 、 Figure 5 and Figure 6 As shown, there are multiple chutes 140, and they are equidistantly arranged along the long side or short side of the box body 100, and the movable partition 300 includes one or more first partitions 340 or second partitions 350. In some embodiments, at least one first partition 340 has the same cross-sectional shape as the long side of the box body 100. In some embodiments, at least one second partition 350 has the same cross-sectional shape as the short side of the box body 100. Slides 140 are arranged in pairs on both sides of the box body 100, and are equipped with multiple partitions. At the same time, the partitions also include first partitions 340 or second partitions 350 arranged along two directions, so that the fresh-keeping storage device can use space more reasonably to adapt to a variety of scenarios. Specifically, the bottom surface of the box body 100 is rectangular, with two longer sides and two shorter sides. When the separated leaves are generally small, a first partition 340 adapted to the cross-sectional shape of the long side of the box body 100 can be used, so that one box body 100 can store more leaves, such as Figure 2 When the separated blades are generally large, a second partition 350 having the same cross-sectional shape as the end face of the box 100 can be used to ensure that the blades can be preserved intact, as shown in FIG. Figure 3 If a plurality of chutes 140 are provided, the partitions can be installed flexibly, and if a plurality of partitions are provided, more partitions can be provided as required to separate more blade storage chambers.

[0043] like Figure 4 As shown, in some embodiments, the slide 140 includes a top protrusion 141, a middle notch and a bottom protrusion 142. There are two top protrusions 141 and two bottom protrusions 142, which are spaced apart and the spacing is adapted to the thickness of the movable partition 300. The middle notch is flush with the inner wall of the box body 100. The middle notch is about to interrupt the slide 140. Removing the middle part of the slide 140 and retaining the top and bottom can reduce the resistance during the installation of the movable partition 300. Specifically, only the top protrusion 141 plays a guiding role when installing the partition, and at the same time, it will generate resistance due to friction. When the movable partition 300 is pushed further into the box body 100, since the middle notch is removed, this part of the slide 140 will not generate additional resistance. The sliding resistance of the movable partition 300 is constant and the sliding is smoother. When it slides to the bottom, it is stuck by the bottom protrusion 142, completely fixing the movable partition 300. In addition, removing the middle portion of the slide groove 140 can avoid interference with the channel 130 (330) that connects the interlayer 110 (310) on the side wall of the box body 100.

[0044] A label pasting place 360 ​​may also be provided on the top side of the first dividing plate 340 or the second dividing plate 350. When the separated leaves are stored, labels may be pasted on the corresponding leaves to mark them for easy differentiation.

[0045] In some embodiments, a first channel 341 and a first fixing rib 342 are provided on one side of the first partition 340 or the second partition 350, and the first fixing rib 342 is used to support the absorbent paper or absorbent sponge 120 (320); a second channel 343 and a second fixing rib 344 are provided on the other side of the first partition 340 or the second partition 350, the area of ​​the second channel 343 is smaller than that of the first channel 341, and the spacing between the second fixing ribs 344 is smaller than that of the first fixing ribs 342, and the second fixing ribs 344 are used to bundle and fix the separated blades; taking the first partition 340 as an example, Figure 5 As shown, an interlayer 310 is provided in the middle of the first partition 340, and the interlayer 310 is connected to the outside through a channel 330. The first channel 341 is relatively large, and its effect is to ensure that the absorbent paper 320 or the absorbent sponge 320 is in contact with the leaves outside the interlayer 310, so as to supply as much water and nutrient solution as possible to the outside. The first fixing rib 342 in the middle of the first channel 341 is used to fix and support the absorbent paper 320 or the absorbent sponge 320 to prevent the absorbent paper 320 or the absorbent sponge 320 from collapsing. The other side of the first partition 340 is provided with denser second fixing ribs 344. When in use, the detached leaves can be bundled on the second fixing ribs 344. At the same time, the second fixing ribs 344 can also fix and support the absorbent paper 320 or the absorbent sponge 320. At the same time, the second channel 343 between the second fixing ribs 344 can also enable the absorbent paper 320 or the absorbent sponge 320 in the interlayer 310 to contact the outside of the interlayer 310, providing nutrients to the leaves and ensuring humidity.

[0046] Since the two sides of the movable partition 300 have different structures, the orientation of the two sides can be flexibly selected during use to adapt to more scenarios.

[0047] The following describes the use of the leaf in vitro preservation device in certain embodiments:

[0048] Step 1: Before use, the box body 100, the box cover 200, and the movable partition 300 of the leaf preservation storage device are disassembled, and the absorbent paper or absorbent sponge 120 (320) is taken out from the movable partition 300 or the box wall and is absorbed to make the humidity 60%-70% or 50%-60%;

[0049] Step 2: Place the absorbent paper 120 or absorbent sponge 120 inserted into the box 100 in a refrigerator for freezing. When collecting in the field, insert the frozen absorbent paper 120 or absorbent sponge 120 into the interlayer 110 of the box 100, close the box lid 200, and carry it with you. Also carry two movable partitions 300 of different sizes and corresponding absorbent paper 320 or absorbent sponges 320.

[0050] Step 3: Leaf Collection and Storage: Use pruning shears to cut orchid leaves 4-12 cm in length. Disinfect the leaves with alcohol and remove any dust from their surfaces. Then remove the movable partition 300 and bundle the orchid leaves onto it. Insert absorbent paper 320 or a sponge 320 with a humidity of 50%-60% into the movable partition 300. Wrap the injured leaves with absorbent paper 320 or a sponge 320 with a humidity of 60%-70% to prevent changes in the physical and chemical properties of the collected samples. Sort the leaves by strain. Place the movable partitions 300 containing leaves of the same strain in the same box 100 and label them at the labeling area 360 for easy identification.

[0051] Step 4: After placing the sample, the first box body 100 and the box cover 200 are buckled together to form a sealed environment relative to the outside for storage.

[0052] Step 5: Bring the storage device and the leaves back to the laboratory or research room and place them in a refrigerator for long-term storage.

[0053] When fresh Huilan leaves were collected through the above-mentioned preservation device, after 4 months, the pigment content comparison table of Huilan leaves was as follows:

[0054]

[0055] The table shows that after placing the sample in the device, bringing it back to the laboratory and storing it in a refrigerator, the leaves can be kept fresh for up to 3-4 months.

[0056] Throughout this specification, reference to terms such as "one embodiment," "certain embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples" means that a specific feature, structure, material, or characteristic described in conjunction with an embodiment or example is included in at least one embodiment or example of the present application. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0057] Although the embodiments of the present application have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions, and variations may be made to the embodiments without departing from the principles and intent of the present application, and that the scope of the present application is defined by the claims and their equivalents.

Claims

1. A device for preserving leaves in vitro, characterized in that: include: Box; A box cover, the box cover is matched with the box body opening; a first moisturizing device and a second moisturizing device, wherein the first moisturizing device and the second moisturizing device are absorbent paper or absorbent sponge for storing nutrient solution; When in use, the humidity of the first moisturizing device is greater than that of the second moisturizing device. The first moisturizing device is used to contact the petiole of the detached leaf, and the second moisturizing device is used to contact the leaf of the detached leaf.

2. The in vitro leaf preservation device according to claim 1, characterized in that: Also includes: A movable partition, located inside the box body, for dividing the internal space of the box body into compartments, wherein the compartments are used to store the separated blades; An interlayer is provided between the side wall of the box body and / or the movable partition, and the interlayer is connected to the outside through a channel; The first moisturizing device and / or the second moisturizing device are located in the interlayer.

3. The in vitro leaf preservation device according to claim 1, characterized in that: When in use, the humidity of the first moisturizing device is 60%-70%, and the humidity of the second moisturizing device is 50%-60%.

4. The in vitro leaf preservation device according to claim 2, characterized in that: The box body and the box cover are cold storage media, and / or; A cold storage medium is provided in the interlayer, and / or; The first moisturizing device and the second moisturizing device are cold storage media.

5. The in vitro leaf preservation device according to claim 2, characterized in that: The long sides and / or short sides of the inner wall of the box are provided with sliding grooves in pairs, and the movable partitions are movably arranged in the sliding grooves.

6. The in vitro leaf preservation device according to claim 5, characterized in that: There are multiple chute slots, which are arranged equidistantly along the long side or short side of the box.

7. The in vitro leaf preservation device according to claim 5, characterized in that: The chute includes a top protrusion, a middle notch and a bottom protrusion; There are two top protrusions and two bottom protrusions, which are spaced apart, and the spacing is adapted to the thickness of the movable partition; The middle notch is flush with the inner wall of the box.

8. The in vitro leaf preservation device according to claim 5, characterized in that: The movable partition includes one or more first partitions or second partitions. The first partition has the same cross-sectional shape as the long side of the box body, and the second partition has the same cross-sectional shape as the short side of the box body.

9. The in vitro leaf preservation device according to claim 8, characterized in that: A label sticking area is provided on the top side of the first partition or the second partition.

10. The in vitro leaf preservation device according to claim 8, characterized in that: A first channel and a first fixing rib are provided on one side of the first partition or the second partition, and the first fixing rib is used to support absorbent paper or absorbent sponge; The other side of the first partition or the second partition is provided with a second channel and a second fixing rib. The area of ​​the second channel is smaller than that of the first channel. The spacing between the second fixing ribs is smaller than that of the first fixing ribs. The second fixing ribs are used to bundle and fix the separated blades.

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