Electric conversion instrument for inducing stem cell editing

Abstract

The utility model discloses an electrotransduction instrument for inducing stem cell editing, which comprises an electrotransduction instrument body, an electric shock cup groove is arranged in the electrotransduction instrument body, a groove cover is arranged above the electric shock cup groove, an adjusting screw rod is arranged in the groove cover in a penetrating manner, a connecting bearing is arranged below the adjusting screw rod, and the connecting bearing is connected with the electrotransduction instrument body. A motor is installed above the adjusting lead screw, limiting auxiliary rods are installed on the two sides of the adjusting lead screw, air conveying branch pipes are installed on the two sides of the electric shock cup groove, and high-pressure nozzles are installed on the sides, close to the electric shock cup groove, of the air conveying branch pipes; and the air pump is installed on the right side of the electrotransformer body, and the output end of the air pump is connected with an air delivery pipe. According to the electric conversion instrument for inducing stem cell editing, the air pump blows air into the electric shock cup groove through the air conveying pipe, the air conveying branch pipe and the high-pressure nozzle, dust in the electric shock cup groove is blown out, and the situation that dust left in the electric shock cup groove influences normal operation of the electric conversion instrument is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell editing, in particular to an electroporator for induced stem cell editing. Background Art

[0002] Induced stem cell editing, especially the editing of induced pluripotent stem cells (iPSCs), has been a research hotspot in the biomedical field in recent years. An electroporator is an essential device for induced stem cell editing. An electroporator, also known as a transformer, is a commonly used instrument in microbiology experiments. It is one of the main methods for introducing exogenous DNA into bacterial cells. The electroporator electroporates the exogenous DNA into the substrate cell, creating temporary holes in the cell membrane through high-voltage electric fields and heat, allowing the DNA to enter the cell.

[0003] China Authorization Announcement No. CN213113356U discloses a cell electroporator for gene editing with a dustproof mechanism, including a dustproof mechanism. The dustproof mechanism is installed on the electroporator body, the electroporator body includes an annular groove, the dustproof mechanism includes a first locking structure and a second locking structure, a dustproof cover is mounted on the annular groove, one side of the dustproof cover is connected to the electroporator body through the first locking structure, and the other side of the dustproof cover is connected to the electroporator body through the second locking structure; by mounting the dustproof cover on the annular groove, external dust can be prevented from entering the electric shock tube in the annular groove, thereby preventing contamination during the cell electroporation process.

[0004] The above-mentioned cell electroporator prevents external dust from entering the annular groove by installing a dust cover. However, when the dust cover is open to place the electroporation cup, dust can easily enter the annular groove, and the electroporation cup may also carry dust. Dust that enters the annular groove is difficult to clean. Therefore, we propose an electroporator for induced stem cell editing. Utility Model Content

[0005] The purpose of the present utility model is to provide an electroporator for induced stem cell editing, so as to solve the problem proposed in the above-mentioned background art that the above-mentioned cell electroporator prevents external dust from entering the annular groove by setting up a dust cover. However, when the dust cover is opened to place the electroporation cup, dust can easily enter the annular groove, and the electroporation cup may also carry dust, and the dust entering the annular groove is not easy to clean.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solutions: an electroporator for induced stem cell editing, comprising an electroporator body, an electroporator cup slot defined within the electroporator body, a slot cover mounted above the electroporator cup slot, an adjustment screw threaded through the slot cover, a connecting bearing mounted below the adjustment screw, a motor mounted above the adjustment screw, limited auxiliary rods mounted on both sides of the adjustment screw, gas supply branches mounted on both sides of the electroporator cup slot, and a high-pressure nozzle mounted on the side of the gas supply branch adjacent to the electroporator cup slot;

[0007] An air pump is installed on the right side of the electrocirculator body, the output end of the air pump is connected to an air pipe, and the input end of the air pump is installed with a purification box.

[0008] Preferably, the adjusting screw is key-connected to the output end of the motor, and the adjusting screw is movably connected to the electric converter body through a connecting bearing, and the adjusting screw is threadedly connected to the slot cover.

[0009] Preferably, the limiting auxiliary rod passes through the interior of the slot cover, and the limiting auxiliary rod is fixedly connected to the electrocirculator body, and the slot cover is moved up and down along the limiting auxiliary rod by adjusting the screw rod.

[0010] Preferably, the gas branch pipe is connected to the gas pipe, and the gas branch pipe is connected to the high-pressure nozzle, and the output end of the high-pressure nozzle is connected to the electric shock cup groove.

[0011] Preferably, the input end of the air pump is connected to the purification box.

[0012] Preferably, the purification box is further provided with:

[0013] An air inlet is provided at the front end of the purification box, a filter is installed inside the air inlet, a snap-fit ​​groove is provided inside the purification box, a filter element is installed inside the snap-fit ​​groove, and a box cover is hinged on the top of the purification box.

[0014] Compared with the prior art, the utility model provides an electroporator for induced stem cell editing, which has the following beneficial effects: the electroporator for induced stem cell editing uses an air pump to blow gas into the interior of the electric shock cup slot through an air pipe, a gas branch pipe and a high-pressure nozzle, thereby blowing out dust inside the electric shock cup slot, thereby preventing dust remaining inside the electric shock cup slot from affecting the normal operation of the electroporator. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the internal structure of the purification box of the utility model;

[0017] Figure 3 This is a schematic diagram of the internal structure of the slot cover of the utility model;

[0018] Figure 4 This is a schematic diagram of the internal structure of the electroporator body of the utility model.

[0019] In the figure: 1. Electric rotator body; 2. Electric shock cup slot; 3. Gas pipe; 31. Gas branch pipe; 32. High-pressure nozzle; 4. Purification box; 41. Box cover; 42. Air inlet; 43. Filter; 44. Engaging slot; 45. Filter element; 5. Air pump; 6. Motor; 61. Adjusting screw; 62. Connecting bearing; 7. Slot cover; 71. Limit auxiliary rod. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0021] See also Figure 1-4The utility model provides an electric transfection instrument for inducing stem cell editing, including electric transfection instrument body 1, the inside of electric transfection instrument body 1 is seted up and is electrified cup slot 2, the upper portion of electrified cup slot 2 is installed with slot cover 7, the inside of slot cover 7 is penetrated with adjusting screw rod 61, and the lower portion of adjusting screw rod 61 is installed with connecting bearing 62;Adjusting screw rod 61 is connected with the output of motor 6 key, and adjusting screw rod 61 is movably connected with electric transfection instrument body 1 through connecting bearing 62, and adjusting screw rod 61 is connected with slot cover 7 screw, and the upper portion of adjusting screw rod 61 is installed with motor 6, and the both sides of adjusting screw rod 61 are installed with limit auxiliary rod 71;Limit auxiliary rod 71 is penetrated in the inside of slot cover 7, and limit auxiliary rod 71 is fixedly connected with electric transfection instrument body 1, and slot cover 7 is moved up and down along limit auxiliary rod 71 through adjusting screw rod 61, and the electrified cup slot 2 is covered by slot cover 7 when not using the electric transfection instrument, avoid the electrified cup slot 2 in the dust, and the both sides of electrified cup slot 2 are installed with gas branch pipe 31, and the side of gas branch pipe 31 close to electrified cup slot 2 is installed with high pressure nozzle 32;Air pump 5 is installed on the right side of electric transfection instrument body 1, and the output of air pump 5 is connected with gas pipe 3;Gas branch pipe 31 is communicated with gas pipe 3, and gas branch pipe 31 is communicated with high pressure nozzle 32, and the output of high pressure nozzle 32 is communicated with electrified cup slot 2;The gas in air pump 5 is blown to the inside of electrified cup slot 2 through gas pipe 3, gas branch pipe 31 and high pressure nozzle 32, and the dust in electrified cup slot 2 is blown out, avoid the dust in electrified cup slot 2 to cause the influence to the normal operation of electric transfection instrument, and the input of air pump 5 is installed with purification tank 4;The input of air pump 5 is communicated with purification tank 4;Air inlet 42 is seted up in the front end of purification tank 4, and filter screen 43 is installed in the inside of air inlet 42, and clamping groove 44 is seted up in the inside of purification tank 4, and filter core 45 is installed in the inside of clamping groove 44, and tank cover 41 is hinged on the upper portion of purification tank 4.

[0022] Working principle: when using the electric transfection instrument for inducing stem cell editing, first, the controller controls the motor 6 to work and drive the adjusting screw rod 61 to rotate, pushing the slot cover 7 to move upwards along the limit auxiliary rod 71, then the controller controls the air pump 5 to work and send air through the gas pipe 3, gas branch pipe 31 and high pressure nozzle 32 to the inside of the electrified cup slot 2 to blow out the dust inside, cleaning the electrified cup slot 2, then put the electrified cup into the inside of the electrified cup slot 2 to work, when the air passes through the purification tank 4, it is filtered first by the filter screen 43 in the air inlet 42, then filtered secondly by the filter core 45, when the filter core 45 filters too much dust, open the tank cover 41, take out the filter core 45 from the inside of the clamping groove 44 to clean or replace, this is the working principle of the electric transfection instrument for inducing stem cell editing.

[0023] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An electroporator for induced stem cell editing, characterized in that: include An electric rotator body (1) is provided with an electric shock cup groove (2) inside the electric rotator body (1), a groove cover (7) is installed above the electric shock cup groove (2), an adjusting screw rod (61) is passed through the groove cover (7), a connecting bearing (62) is installed below the adjusting screw rod (61), a motor (6) is installed above the adjusting screw rod (61), limiting auxiliary rods (71) are installed on both sides of the adjusting screw rod (61), gas supply branches (31) are installed on both sides of the electric shock cup groove (2), and a high-pressure nozzle (32) is installed on the side of the gas supply branch pipe (31) close to the electric shock cup groove (2); An air pump (5) is installed on the right side of the electroconverter body (1), the output end of the air pump (5) is connected to an air delivery pipe (3), and the input end of the air pump (5) is installed with a purification box (4).

2. The electroporator for induced stem cell editing according to claim 1, characterized in that: The adjusting screw rod (61) is key-connected to the output end of the motor (6), and the adjusting screw rod (61) is movably connected to the electro-rotator body (1) through a connecting bearing (62), and the adjusting screw rod (61) is threadedly connected to the slot cover (7).

3. The electroporator for induced stem cell editing according to claim 1, characterized in that: The limiting auxiliary rod (71) passes through the interior of the slot cover (7), and the limiting auxiliary rod (71) is fixedly connected to the electro-rotator body (1). The slot cover (7) is moved up and down along the limiting auxiliary rod (71) by adjusting the screw rod (61).

4. The electroporator for induced stem cell editing according to claim 1, characterized in that: The gas delivery branch pipe (31) is in communication with the gas delivery pipe (3), the gas delivery branch pipe (31) is in communication with the high-pressure nozzle (32), and the output end of the high-pressure nozzle (32) is in communication with the electric shock cup tank (2).

5. The electroporator for induced stem cell editing according to claim 1, characterized in that: The input end of the air pump (5) is communicated with the purification box (4).

6. The electroporator for induced stem cell editing according to claim 1, characterized in that: The purification box (4) is also provided with: An air inlet (42) is provided at the front end of the purification box (4), a filter screen (43) is installed inside the air inlet (42), a snap-fit ​​groove (44) is provided inside the purification box (4), a filter element (45) is installed inside the snap-fit ​​groove (44), and a box cover (41) is hinged on the top of the purification box (4).

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