Tissue dissociation tube and dissociation device
By using a combination structure of multiple first grinding bodies and cutting bodies in a tissue dissociation device, the problems of high cost and unsatisfactory dispersion in preparing single cell suspensions in the prior art are solved, and efficient and low-cost single cell suspension preparation is achieved.
Patent Information
- Application Number
- CN202422391201.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-29
- Publication Date
- 2025-10-10
- Estimated Expiration
- 2034-09-29
AI Technical Summary
In the prior art, the devices for preparing single cell suspensions are relatively expensive and have complex structures. Moreover, when the dissociation is insufficient, the dispersion of the obtained cell suspension is not ideal.
A tissue dissociation device is used, including a base and a rotating part. The rotating part includes multiple first grinding bodies arranged along the circumferential direction of the inner cylinder to form a continuous stepped structure. The rotating base drives the second grinding body and the cutting body to rotate, thereby achieving sufficient grinding and cutting of the tissue.
A single cell suspension with high uniformity and good dispersion is obtained, and the device has a simple structure and low cost.
Smart Images
Figure CN223422676U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to a tissue separation device, and in particular to a tissue dissociation tube and a dissociation device having the tissue dissociation tube. Background Art
[0002] Tissue dissociation is a technique for breaking down and disaggregating bulk animal and plant tissue into single-cell suspensions for subsequent capture, lysis, or other research procedures. The dispersion of cells in a single-cell suspension directly impacts its quality. However, the equipment used to prepare single-cell suspensions in related technologies is costly and complex, and insufficient dissociation can result in suboptimal dispersion of the resulting single-cell suspension. Utility Model Content
[0003] In view of the above, it is necessary to provide a tissue dissociation tube and a dissociation device.
[0004] In a first aspect, the present application provides a tissue dissociation tube comprising a base and a rotating member. The base comprises an inner cylinder and a plurality of first grinding bodies. The plurality of first grinding bodies are connected to the inner sidewall of the inner cylinder and arranged along the circumference of the inner cylinder, and the plurality of first grinding bodies enclose a dissociation pool. The rotating member comprises a rotating base, a rotating shaft, a second grinding body, and a cutting body. The rotating base is rotatably connected to the inner cylinder. The rotating shaft is fixed to the rotating base and is at least partially located within the dissociation pool. The second grinding body is fixed to the rotating shaft and extends helically from one end of the rotating shaft proximal to the rotating base to an end distal to the rotating base. The second grinding body and the first grinding body are loosely fitted in the radial direction of the rotating shaft. The cutting body is fixed to the rotating shaft and extends radially from the rotating shaft. The projection of the cutting body on the rotating base along the axis of the rotating shaft at least partially does not overlap with the projection of the second grinding body on the rotating base along the axis of the rotating shaft.
[0005] Based on the first aspect, in some possible implementations, there are multiple cutting bodies, which are spaced apart along the axis of the rotating shaft, and projections of two adjacent cutting bodies along the axis of the rotating shaft on the rotating base overlap.
[0006] Based on the first aspect, in some possible implementations, the cutting body includes a first connecting edge and a first cutting edge. The first connecting edge is connected to the rotating shaft. The first cutting edge is connected to the first cutting edge and is disposed along the circumferential direction of the rotating shaft toward the outer side surface of the second grinding body. The first cutting edge is provided with a plurality of first cutting teeth.
[0007] Based on the first aspect, in some possible implementations, the tissue dissociation tube further includes a cover member detachably connected to the base. The cover member is provided with a cover groove for accommodating tissue, and the cover groove is configured to communicate with the dissociation tank. The second grinding body includes a grinding portion located within the dissociation tank and a guide portion extending from a side of the grinding portion away from the rotating base to outside the dissociation tank. The guide portion is configured to guide tissue within the cover groove into the dissociation tank.
[0008] Based on the first aspect, in some possible implementations, the grinding portion includes a radial portion and a circumferential portion. The radial portion is connected to the rotating shaft and extends radially from the rotating shaft to the edge of the rotating base. The circumferential portion is smoothly connected to a side of the radial portion away from the rotating shaft. The circumferential portion is connected to the edge of the rotating base and is arranged along the circumference of the rotating base.
[0009] Based on the first aspect, in some possible implementations, the rotating member further includes a plurality of third grinding bodies connected to an edge of the rotating base. The plurality of third grinding bodies are spaced apart along the circumference of the rotating base. The third grinding bodies are clearance-fitted with the first grinding body in a radial direction of the rotating axis. The circumferential portion and the third grinding bodies are disposed together around the cutting body.
[0010] Based on the first aspect, in some possible implementations, the third grinding body includes a second connecting edge and a second cutting edge. The second connecting edge is connected to an edge of the rotating base. The second cutting edge is connected to the second connecting edge and is disposed along the circumference of the rotating base toward the outer side surface of the second grinding body. The second cutting edge is provided with a plurality of second cutting teeth.
[0011] Based on the first aspect, in some possible implementations, the dissociation tank is tapered and barrel-shaped, with a radius of the dissociation tank gradually increasing from an end of the rotation axis closer to the rotating base to an end farther from the rotating base. The circumferential portion and the third grinding body are both tapered and barrel-shaped and compatible with the dissociation tank.
[0012] Based on the first aspect, in some possible implementations, the tissue dissociation tube further includes a shaft sleeve, which is disposed between the rotating base and the inner cylinder.
[0013] A second aspect of the present application provides a dissociation device comprising the tissue dissociation tube and a driving member, wherein the driving member is configured to be detachably connected to a rotating member of the tissue dissociation tube and to drive the rotating member to rotate.
[0014] The tissue dissociation tube and dissociation device of the present application can dissociate tissue to obtain a single-cell suspension, wherein a plurality of first grinding bodies are arranged along the circumferential direction of the inner cylinder to form a continuous stepped structure. When the rotating base rotates relative to the inner cylinder, it drives the second grinding body to rotate, and the second grinding body cooperates with the first grinding body to grind and squeeze the tissue in the dissociation tank. Moreover, when the rotating base rotates relative to the inner cylinder, it also drives the cutting body to rotate to cut the tissue in the dissociation tank. Therefore, the first grinding body, the second grinding body and the cutting body work together to allow the tissue to be fully ground and cut to obtain a single-cell suspension with high uniformity and good dispersion. Moreover, the above-mentioned tissue dissociation tube and dissociation device have a simple structure and low cost. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 This is a schematic structural diagram of a tissue dissociation tube provided in one embodiment of the present application.
[0016] Figure 2 for Figure 1 Schematic diagram of the structure of the tissue dissociation tube from another angle.
[0017] Figure 3 for Figure 1 The tissue dissociation tube is shown in a cross-sectional view along the cutting line III-III.
[0018] Figure 4 for Figure 1 Exploded view of the tissue dissociation tube shown.
[0019] Figure 5 for Figure 4 Schematic diagram of the structure of the rotating part of the tissue dissociation tube shown.
[0020] Figure 6 for Figure 4 Schematic diagram of the structure of the base of the tissue dissociation tube is shown.
[0021] Figure 7 for Figure 5 and Figure 6 The schematic diagram of the structure after the rotating part and the base are connected is shown.
[0022] Figure 8 This is a schematic structural diagram of a dissociation device provided in one embodiment of the present application.
[0023] Description of main component symbols
[0024] Dissociation device 1
[0025] Base 10
[0026] Inner tube 11
[0027] Outer cylinder 12
[0028] Connecting portion 13
[0029] First grinding body 14
[0030] Rotating member 20
[0031] Rotating base 21
[0032] Rotating shaft 22
[0033] Second grinding body 23
[0034] Cutting body 24
[0035] The third grinding body 25
[0036] Covering member 30
[0037] Covering slot 31
[0038] External thread 32
[0039] Seal 40
[0040] Sleeve 50
[0041] Tissue dissociation tube 100
[0042] First cylinder 111
[0043] Second cylinder 112
[0044] Internal thread 120
[0045] Dissociation cell 140
[0046] Driving member 200
[0047] Mounting hole 210
[0048] Grinding section 231
[0049] Guide portion 232
[0050] First connecting edge 240
[0051] First cutting edge 241
[0052] Second connecting edge 250
[0053] Second cutting edge 251
[0054] Radial portion 2311
[0055] Circumferential portion 2312
[0056] First cutting tooth 2410
[0057] Second cutting tooth 2510
[0058] First direction X
[0059] Second direction Y DETAILED DESCRIPTION
[0060] The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application, and should not be understood as limiting the present application.
[0061] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the said features. In the description of the present application, "multiple" means two or more, unless otherwise clearly and specifically defined.
[0062] In the description of this application, it should be noted that, unless otherwise expressly specified or limited, the terms "installed," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections, electrical connections, or mutual communication; they can refer to direct connections or indirect connections through an intermediate medium; they can refer to internal communication between two components or the interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in this application based on specific circumstances.
[0063] In this application, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Moreover, a first feature being "above," "above," and "above" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicates that the first feature is lower in level than the second feature.
[0064] Please also see Figures 1 to 4 In one embodiment of the present application, a tissue dissociation tube 100 is provided for dissociating tissue to obtain a single cell suspension. The tissue dissociation tube 100 comprises a base 10 and a rotating member 20 ( Figure 3 and Figure 4 shown in ).
[0065] like Figure 3 、 Figure 4 and Figure 6 As shown, the base 10 includes an inner cylinder 11 and a plurality of first grinding bodies 14. The inner cylinder 11 is hollow, and the plurality of first grinding bodies 14 are connected to the inner sidewall of the inner cylinder 11 and arranged along the circumference of the inner cylinder 11. The first grinding bodies 14 are protruding from the inner sidewall of the inner cylinder 11. The plurality of first grinding bodies 14 enclose a dissociation tank 140, in which tissue is dissociated to obtain a single-cell suspension. In some embodiments, the cross-section of the first grinding bodies 14 is generally triangular.
[0066] like Figures 3 to 5 As shown, the rotating member 20 includes a rotating base 21, a rotating shaft 22, a second grinding body 23 and a cutting body 24. The rotating base 21 is rotatably connected to the inner cylinder 11, and the rotating base 21 can be driven by a driving member 200 (in Figure 8 The rotating shaft 22 is fixed to the rotating base 21 and is at least partially located in the dissociation tank 140. In some embodiments, the dissociation tank 140 is in a conical barrel shape, and the radius of the dissociation tank 140 gradually increases from the end of the rotating shaft 22 close to the rotating base 21 to the end away from the rotating base 21. Therefore, it is convenient for the dissociation tank 140 to accommodate the tissue to be dissociated and prevent the tissue from being lost from the inner cylinder 11 during the dissociation process. Figure 3As shown, in some embodiments, the inner cylinder 11 includes a first cylinder 111 and a second cylinder 112 connected to each other. Along the second direction Y, the width of the first cylinder 111 is greater than the width of the second cylinder 112. The first grinding body 14 is connected to the inner side wall of the first cylinder 111, that is, the dissociation tank 140 formed by the plurality of first grinding bodies 14 is located in the first cylinder 111. At least part of the rotating base 21 is rotatably disposed in the second cylinder 112, and the rotating shaft 22 extends into the first cylinder 111. In some embodiments, the side of the rotating base 21 facing away from the rotating shaft 22 can be connected to the driving member 200. The base 10, the rotating base 21 and the rotating shaft 22 can all be roughly cylindrical structures.
[0067] The second grinding body 23 is fixed to the rotating shaft 22. The second grinding body 23 spirally extends from one end of the rotating shaft 22 close to the rotating base 21 to the other end away from the rotating base 21, and the second grinding body 23 and the first grinding body 14 are loosely matched along the radial direction of the rotating shaft 22. The second grinding body 23 and the first grinding body 14 can cooperate to grind and squeeze the tissue in the dissociation tank 140. The cutting body 24 is fixed to the rotating shaft 22 and preferably extends from the rotating shaft 22 along the radial direction of the rotating shaft 22. Figure 5 and Figure 7 As shown, the projection of the cutting body 24 on the rotating base 21 along the axis of the rotating shaft 22 and the projection of the second grinding body 23 on the rotating base 21 along the axis of the rotating shaft 22 at least partially do not overlap, and preferably do not overlap. The cutting body 24 can cut the tissue in the dissociation tank 140. The fact that the cutting body 24 extends radially along the rotating shaft 22 does not mean that the cutting body 24 must be perpendicular to the first direction X. In some embodiments, the cross-section of the first grinding body 14 along the axis perpendicular to the rotating shaft 22 is approximately triangular.
[0068] The coordinate axes are established by a first direction X and a second direction Y, which are perpendicular to each other. The first direction X is the axial direction of the rotating shaft 22, and the second direction Y is the radial direction of the rotating shaft 22. In some embodiments, the second grinding body 23 exhibits a predetermined spiral direction, and this spiral direction can be left-handed. That is, the second grinding body 23 spirals upward in a counterclockwise direction (in a direction from the end of the rotating shaft 22 closest to the rotating base 21 to the end farther from the rotating base 21). The rotating member 20 can rotate relative to the inner cylinder 11 in a direction opposite to this spiral direction.
[0069] like Figures 1 to 4 As shown, in some embodiments, the tissue dissociation tube 100 further includes a cover member 30 detachably connected to the base 10. The cover member 30 is provided with a cover slot 31 (in Figure 3(shown in the figure). When the cover member 30 is connected to the base 10, the cover groove 31 communicates with the dissociation tank 140, allowing the tissue contained in the cover groove 31 to enter the dissociation tank 140 for grinding and cutting. The radius of the cover member 30 can gradually decrease from the end of the rotating shaft 22 close to the rotating base 21 to the end away from the rotating base 21, thereby facilitating the tissue to sink into the dissociation tank 140 during tissue dissociation and fully contact the first grinding body 14, the second grinding body 23, and the cutting body 24. The cover member 30 can be made of a transparent material to visualize the tissue dissociation process.
[0070] The usage process of the above-mentioned tissue dissociation tube 100 is roughly as follows: first, the tissue is placed in the cover 30, and the cover 30 is fixed between the outer cylinder 12 and the inner cylinder 11 through the cover 30 or the base 10. At this time, the opening of the cover 30 of the tissue dissociation tube 100 is set upward; then, the tissue dissociation tube 100 is inverted, and the end of the rotating base 21 away from the rotating shaft 22 is connected to the driving member 200; the driving member 200 drives the rotating base 21 to rotate relative to the inner cylinder 11. During the rotation process, the tissue located in the dissociation pool 140 is ground and squeezed multiple times between the second grinding body 23 and the first grinding body 14, and the cutting body 24 cuts the tissue in the dissociation pool 140; after a preset time, the driving member 200 is turned off, the tissue dissociation tube 100 is inverted again and the cover 30 is separated from the base 10; finally, the formed single cell suspension is poured out.
[0071] The tissue dissociation tube 100 of the present application can dissociate tissue to obtain a single-cell suspension. Multiple first grinding bodies 14 are arranged along the circumference of an inner cylinder 11 to form a continuous stepped structure. When the rotating base 21 rotates relative to the inner cylinder 11, it drives the second grinding bodies 23 to rotate. The second grinding bodies 23 cooperate with the first grinding bodies 14 to grind and compress the tissue in the dissociation tank 140. Furthermore, when the rotating base 21 rotates relative to the inner cylinder 11, it also drives the cutting bodies 24 to rotate, thereby cutting the tissue in the dissociation tank 140. Therefore, the first grinding bodies 14, the second grinding bodies 23, and the cutting bodies 24 work together to fully grind and cut the tissue to obtain a highly uniform and well-dispersed single-cell suspension. Furthermore, by ensuring that the projections of the cutting bodies 24 and the second grinding bodies 23 along the axis of the rotating shaft 22 do not at least partially overlap, the cutting efficiency of the cutting bodies 24 can be improved. Furthermore, the tissue dissociation tube 100 and dissociation device 1 described above have a simple structure and are relatively low in cost.
[0072] like Figure 4 and Figure 5As shown, in some embodiments, a plurality of cutting bodies 24 may be provided to improve the efficiency of cutting tissue within the dissociation tank 140. The plurality of cutting bodies 24 are spaced apart along the first direction X, and the projections of two adjacent cutting bodies 24 along the first direction X on the rotating base 21 overlap. In some embodiments, the plurality of cutting bodies 24 are arranged in a spiral from one end of the rotating shaft 22 close to the rotating base 21 to the other end away from the rotating base 21, and the direction of the spiral arrangement of the plurality of cutting bodies 24 is the same as the spiral direction of the second grinding body 23, that is, the plurality of cutting bodies 24 are arranged in a left-hand spiral. This can reduce the obstruction of the sinking tissue by the cutting bodies 24 as the tissue contained in the cover 30 sinks into the dissociation tank 140. In some specific embodiments, the number of cutting bodies 24 is five.
[0073] The cutting body 24 may be a generally sheet-like structure, with its plane being perpendicular to the first direction X. The cutting body 24 may include a first connecting edge 240 and a first cutting edge 241. The first connecting edge 240 is connected to the rotating shaft 22. The first cutting edge 241 is connected to the first cutting edge 241 and is arranged along the circumferential direction of the rotating shaft 22 toward the outer side surface of the second grinding body 23. When the rotating base 21 rotates relative to the inner cylinder 11 along the rotation direction, the first cutting edge 241 is located at the front end of the cutting body 24 along the above-mentioned rotation direction. The first cutting edge 241 is provided with a plurality of first cutting teeth 2410, thereby further improving the cutting efficiency of the tissue in the dissociation tank 140.
[0074] Combine Figures 5 to 7 In some embodiments, the second grinding body 23 includes a grinding portion 231 located within the dissociation tank 140 and a guide portion 232 extending from the side of the grinding portion 231 away from the rotating base 21 to the outside of the dissociation tank 140. The guide portion 232 can guide the tissue in the capping groove 31 into the dissociation tank 140. The grinding portion 231 can cooperate with the first grinding body 14 to grind and compress the tissue in the dissociation tank 140. The spiral extension of the grinding portion 231 forms an arc-shaped transition space between the grinding portion 231 and the first grinding body 14, which can compress and grind larger pieces of tissue.
[0075] like Figure 3As shown, the base 10 may further include an outer cylinder 12 and a connecting portion 13. The outer cylinder 12 is sleeved on the outside of the inner cylinder 11, and the outer cylinder 12 and the inner cylinder 11 can be connected by the connecting portion 13. For example, along a cross section parallel to the first direction X, the connecting portion 13 can be roughly L-shaped, with one end of the connecting portion 13 connected to the inner side wall of the outer cylinder 12, and the other end of the connecting portion 13 connected to the outer side wall of the inner cylinder 11. The outer cylinder 12 and the connecting portion 13 are spaced apart along the second direction Y. The inner side wall of the outer cylinder 12 is provided with an internal thread 120, and the outer side wall of the cover member 30 is provided with an external thread 32. The cover member 30 can be inserted into the gap between the outer cylinder 12 and the connecting portion 13 and sleeved on the outer circumference of the inner cylinder 11, and the external thread 32 of the cover member 30 cooperates with the internal thread 120 of the outer cylinder 12, so that the cover member 30 and the base 10 are detachably connected. The cover 30 and the base 10 are assembled by threaded connection, which makes installation convenient and production cost low.
[0076] like Figure 5 As shown, in some embodiments, the grinding portion 231 includes a radial portion 2311 and a circumferential portion 2312. The radial portion 2311 is connected to the rotating shaft 22 and extends along the second direction Y to the edge of the rotating base 21. The circumferential portion 2312 is smoothly connected to the side of the radial portion 2311 away from the rotating shaft 22. The circumferential portion 2312 is connected to the edge of the rotating base 21 and is arranged along the circumferential direction of the rotating base 21.
[0077] Furthermore, the rotating member 20 may further include a plurality of third grinding bodies 25 connected to the edge of the rotating base 21. The plurality of third grinding bodies 25 are spaced apart along the circumference of the rotating base 21. The third grinding bodies 25 are spaced apart from each other along the second direction Y with the first grinding body 14. The circumferential portion 2312 and the third grinding bodies 25 are disposed together around the cutting body 24. Therefore, the third grinding bodies 25 can cooperate with the first grinding body 14 to grind and compress the tissue in the dissociation tank 140, thereby improving the efficiency of tissue grinding. In some embodiments, there are three third grinding bodies 25.
[0078] The third grinding body 25 may include a second connecting edge 250 and a second cutting edge 251. The second connecting edge 250 is connected to the edge of the rotating base 21. The second cutting edge 251 is connected to the second connecting edge 250 and is arranged along the circumferential direction of the rotating base 21 toward the outer side of the second grinding body 23. When the rotating base 21 rotates relative to the inner cylinder 11 along the rotation direction, the second cutting edge 251 is located at the front end of the third grinding body 25 along the above-mentioned rotation direction. The second cutting edge 251 is provided with a plurality of second cutting teeth 2510. Therefore, when the third grinding body 25 and the first grinding body 14 can cooperate to grind and squeeze the tissue in the dissociation tank 140, the third grinding body 25 can also cut the tissue, thereby further improving the cutting efficiency of the tissue in the dissociation tank 140.
[0079] When the dissociation tank 140 is tapered, the circumferential portion 2312 and the third grinding body 25 are both tapered and compatible with the dissociation tank 140. Specifically, the radius of the cone formed by the circumferential portion 2312 and the third grinding body 25 gradually increases from the end of the rotating shaft 22 closest to the rotating base 21 to the end farther from the rotating base 21. This improves tissue grinding efficiency.
[0080] like Figure 3 and Figure 4 As shown, in some embodiments, the tissue dissociation tube 100 may further include a seal 40, and the seal 40 is disposed between the inner cylinder 11 and the rotating base 21. The seal 40 can seal the gap between the inner cylinder 11 and the rotating base 21, thereby preventing tissue from flowing out of the gap during the dissociation process. In some embodiments, the seal 40 can be disposed at the junction of the first cylinder 111 and the second cylinder 112, and the seal 40 can be a sealing ring made of an elastic material such as silicone or rubber.
[0081] The tissue dissociation tube 100 may further include a sleeve 50, which is disposed between the rotating base 21 and the inner cylinder 11. The sleeve 50 has a self-lubricating effect, which can reduce friction of the rotating base 21 during rotation, thereby ensuring smooth and stable rotation. In some embodiments, the sleeve 50 can be disposed between the rotating base 21 and the second cylinder 112.
[0082] See also Figure 8 Another embodiment of the present application further provides a dissociation device 1, comprising a driving member 200 and the tissue dissociation tube 100. The driving member 200 is detachably connected to a side of a rotating base 21 facing away from the rotating shaft 22, and the driving member 200 can drive the rotating member 20 to rotate via the rotating base 21. In some embodiments, the driving member 200 can be a motor.
[0083] In some embodiments, the driving member 200 and the rotating base 21 can be positioned by means of a buckle or positioning beads (not shown), thereby preventing relative movement between the driving member 200 and the rotating base 21 along the first direction X. Furthermore, a mounting hole 210 is provided on a side of the rotating base 21 facing away from the rotating axis 22, and the mounting hole 210 is polygonal (e.g., hexagonal) when viewed from the first direction X. At least a portion of the driving member 200 is inserted into and fits into the mounting hole 210, thereby preventing relative movement between the driving member 200 and the rotating base 21 along the rotational direction.
[0084] The dissociation device 1 of the present application can dissociate tissue to obtain a single-cell suspension, wherein a plurality of first grinding bodies 14 are arranged along the circumferential direction of the inner cylinder 11 to form a continuous stepped structure. When the rotating base 21 rotates relative to the inner cylinder 11, it drives the second grinding body 23 to rotate. The second grinding body 23 cooperates with the first grinding body 14 to grind and squeeze the tissue in the dissociation tank 140. Moreover, when the rotating base 21 rotates relative to the inner cylinder 11, it also drives the cutting body 24 to rotate to cut the tissue in the dissociation tank 140. Therefore, the first grinding body 14, the second grinding body 23 and the cutting body 24 work together to allow the tissue to be fully ground and cut to obtain a single-cell suspension with high uniformity and good dispersion. Moreover, the above-mentioned tissue dissociation tube 100 and dissociation device 1 have a simple structure and low cost.
[0085] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and are not intended to limit the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application.
Claims
1. A tissue dissociation tube, characterized in that: include: A base, comprising an inner cylinder and a plurality of first grinding bodies, wherein the plurality of first grinding bodies are connected to the inner side wall of the inner cylinder and arranged along the circumferential direction of the inner cylinder, and the plurality of first grinding bodies enclose a dissociation pool; The rotating member includes a rotating base, a rotating shaft, a second grinding body and a cutting body, wherein the rotating base is rotatably connected to the inner cylinder, the rotating shaft is fixed to the rotating base and is at least partially located in the dissociation tank; the second grinding body is fixed to the rotating shaft, and the second grinding body spirally extends from one end of the rotating shaft close to the rotating base to one end away from the rotating base, and the second grinding body is clearance-fitted with the first grinding body along the radial direction of the rotating shaft; the cutting body is fixed to the rotating shaft, and the cutting body extends from the rotating shaft along the radial direction of the rotating shaft, and the projection of the cutting body on the rotating base along the axial direction of the rotating shaft and the projection of the second grinding body on the rotating base along the axial direction of the rotating shaft at least partially do not overlap.
2. The tissue dissociation tube according to claim 1, wherein There are multiple cutting bodies, and the multiple cutting bodies are arranged at intervals along the axial direction of the rotating shaft. The projections of two adjacent cutting bodies along the axial direction of the rotating shaft on the rotating base overlap.
3. The tissue dissociation tube according to claim 1 or 2, wherein: The cutting body includes a first connecting edge and a first cutting edge, the first connecting edge is connected to the rotating shaft, the first cutting edge is connected to the first cutting edge and is arranged along the circumferential direction of the rotating shaft toward the outer side surface of the second grinding body, and the first cutting edge is provided with a plurality of first cutting teeth.
4. The tissue dissociation tube according to claim 1, wherein The tissue dissociation tube further comprises a sealing member detachably connected to the base, wherein the sealing member is provided with a sealing groove for accommodating tissue, and the sealing groove is configured to communicate with the dissociation tank; The second grinding body includes a grinding portion located in the dissociation tank and a guide portion extending from a side of the grinding portion away from the rotating base to outside the dissociation tank, and the guide portion is configured to guide the tissue in the capping groove into the dissociation tank.
5. The tissue dissociation tube according to claim 4, wherein: The grinding portion includes a radial portion and a circumferential portion, the radial portion is connected to the rotating shaft and extends along the radial direction of the rotating shaft to the edge of the rotating base, the circumferential portion is smoothly connected to the side of the radial portion away from the rotating shaft, the circumferential portion is connected to the edge of the rotating base and is arranged along the circumferential direction of the rotating base.
6. The tissue dissociation tube according to claim 5, wherein: The rotating member also includes a plurality of third grinding bodies connected to the edge of the rotating base, the plurality of third grinding bodies are arranged at intervals along the circumferential direction of the rotating base, the third grinding bodies are clearance-matched with the first grinding bodies along the radial direction of the rotating axis, and the circumferential portion and the third grinding bodies are arranged together around the cutting body.
7. The tissue dissociation tube according to claim 6, wherein: The third grinding body includes a second connecting edge and a second cutting edge, the second connecting edge is connected to the edge of the rotating base, the second cutting edge is connected to the second connecting edge and is arranged along the circumferential direction of the rotating base toward the outer side surface of the second grinding body, and the second cutting edge is provided with a plurality of second cutting teeth.
8. The tissue dissociation tube according to claim 6, wherein: The dissociation tank is in a conical barrel shape, and the radius of the dissociation tank gradually increases from the end of the rotating shaft close to the rotating base to the end away from the rotating base; the circumferential portion and the third grinding body are both in a conical barrel shape and are adapted to the dissociation tank.
9. The tissue dissociation tube according to claim 1, wherein: The tissue dissociation tube further comprises a shaft sleeve, which is arranged between the rotating base and the inner cylinder.
10. A dissociation device, characterized in that: include: The tissue dissociation tube according to any one of claims 1 to 9; as well as The driving member is configured to be detachably connected to the rotating member of the tissue dissociation tube and drive the rotating member to rotate.