Automatic medium-pressure preparation and separation equipment and automatic separation and preparation system

Abstract

The utility model provides automatic medium-pressure preparation and separation equipment and an automatic separation and preparation system. The automatic medium-pressure preparation and separation equipment comprises a workbench; the sample loading, liquid adding and needle cleaning module, the column packing module, the fraction collecting module, the carrying mechanism and the pump valve module are arranged on the workbench; the mobile phase cache module, the sample loading liquid adding and needle cleaning module which are arranged independent of the workbench are used for sucking sample liquid and cleaning a sample loading needle; chromatographic columns and / or sample loading columns are installed on the column loading module through a carrying mechanism, samples are separated, and the chromatographic columns and / or the sample loading columns of various specifications can be arranged in the column loading module; the fraction collection module is used for collecting fractions; the carrying mechanism is used for transferring materials on the sample loading, liquid adding and needle cleaning module, the column packing module and the fraction collecting module; the mobile phase cache module caches the required mobile phase; and the pump valve module is respectively communicated with the sample loading, liquid adding and needle cleaning module, the column packing module, the fraction collecting module and the mobile phase caching module and is used for providing power for liquid transmission among the modules. According to the invention, automation and unmanned operation of the whole process of medium-pressure preparation and separation are realized.

Description

TECHNICAL FIELD

[0001] The present disclosure relates to the technical field of automation equipment, in particular to the technical field of automation equipment for medicine and chemical industry, and more particularly to an automatic medium-pressure preparation separation equipment and an automatic separation preparation system. BACKGROUND

[0002] Experiments in biological and pharmaceutical industries often involve a large number of component separation and purification processes. Chromatography is a technology that separates and purifies different substances by using different distribution coefficients between stationary phase and mobile phase. In the research and production of traditional Chinese medicine, chromatography is widely used in the separation, purification, identification and quality control of complex traditional Chinese medicine components. At present, a large amount of manual intervention is required during the separation and purification of components, resulting in high labor cost, low processing efficiency and difficulty in ensuring the accuracy of experiments. CONTENT OF THE INVENTION

[0003] In view of the above problems, the present disclosure provides an automatic medium-pressure preparation separation equipment and an automatic separation preparation system. By using automatic liquid suction and column loading processes, the speed of sample processing is greatly improved, a large number of samples can be processed in a short time, human errors are reduced, the consistency of the volume and rate of each liquid suction and column loading is ensured, and the efficiency, accuracy and safety of experiments are improved. At the same time, labor cost and potential human errors are reduced, and laboratory work is more efficient and standardized.

[0004] In a first aspect, the present disclosure provides an automatic medium-pressure preparation separation equipment, comprising: a workbench; a sample loading and needle cleaning module, a column loading module, a fraction collection module, a conveying mechanism and a pump valve module arranged on the workbench; and a mobile phase buffer module arranged independently of the workbench, wherein the sample loading and needle cleaning module is used for sucking sample liquid and cleaning the sample needle; the column loading module is used for installing a chromatographic column and / or a sample column to the column loading module through the conveying mechanism to separate the components of the sample, and the column loading module can accommodate chromatographic columns and / or sample columns of multiple specifications; the fraction collection module is used for collecting fractions; the conveying mechanism is used for transferring materials among the sample loading and needle cleaning module, the column loading module and the fraction collection module; the mobile phase buffer module is used for buffering and placing the required mobile phase; and the pump valve module is in communication with the sample loading and needle cleaning module, the column loading module, the fraction collection module and the mobile phase buffer module, respectively, and provides power for liquid transmission between the modules.

[0005] In some embodiments, the automatic medium-pressure preparation separation equipment further comprises an interaction unit for interacting with the outside through the conveying mechanism; a cleaning column module for buffering and placing a cleaning column; and a liquid leakage sensing module for detecting liquid leakage and issuing a real-time alarm.

[0006] In some embodiments, the automated medium-pressure preparative separation equipment further includes a storage rack for placing materials required for processing in each module; and an upper plug placement module for caching and placing the upper plugs of the chromatographic column and / or loading column.

[0007] In some embodiments, the mobile phase buffer module includes a plurality of mobile phase buffer boxes, in which large-capacity barrels filled with mobile phase are placed.

[0008] In some embodiments, the interactive unit is arranged on the side of the workbench close to the user operation, and the sample loading and liquid addition and needle cleaning module, column packing module, and fraction collection module are arranged in sequence adjacent to the transport mechanism.

[0009] In some embodiments, the sample loading and liquid addition and needle washing module includes a sample loading and liquid addition part and a sample loading needle washing part.

[0010] In some embodiments, the sample loading and liquid adding part includes a lifting mechanism, a sample loading needle arranged on the lifting mechanism, a pump connected to the sample loading needle, a container seat located below the sample loading needle, and a translation mechanism capable of driving the container seat to move, and a container containing the sample liquid is placed in the container seat.

[0011] In some embodiments, the sample loading needle cleaning part includes a cleaning seat, and a first cleaning channel, a second cleaning channel and a drainage channel arranged in the cleaning seat, the first cleaning channel is used to clean the outer wall of the sample loading needle, the second cleaning channel is used to clean the inner wall of the sample loading needle, and the drainage channel is used to discharge the waste liquid after cleaning. The first cleaning channel and the second cleaning channel are separated and connected, and the second cleaning channel is connected to the drainage channel.

[0012] In some embodiments, one end of the first cleaning channel has a first inlet for the sample loading needle to enter, and the other end is closed. One end of the second cleaning channel has a second inlet for the sample loading needle to enter, and the other end is connected to the drainage channel. The first inlet is connected to the second inlet. The drainage channel has a drainage port, and the drainage port is connected to the waste liquid bottle.

[0013] In some embodiments, the column loading module includes a first movable seat, a second movable seat, and a fixed seat arranged in sequence from top to bottom; a first driving mechanism for driving the first movable seat to rise and fall; and a second driving mechanism for driving the second movable seat to rise and fall, the first movable seat and the second movable seat are opposite to each other, the second movable seat and the fixed seat are opposite to each other, the first movable seat is provided with multiple first joints, the second movable seat is provided with multiple second joints, and the fixed seat is provided with multiple third joints, the specifications of the multiple first joints are the same or different, the specifications of the multiple second joints are the same or different, and the specifications of the multiple third joints are the same or different.

[0014] In some embodiments, one end of the first joint is in communication with the sample loading and liquid addition module and the needle cleaning module, and one end of the third joint is in communication with the fraction collection module through the pump valve module.

[0015] In some embodiments, the first driving mechanism comprises a first transmission member and a first driving member, the first transmission member connecting the first driving member and the first movable seat, the first driving member driving the first movable seat to move up and down via the first transmission member, and the second driving mechanism comprises a second transmission member and a second driving member, the second transmission member connecting the second driving member and the second movable seat, the second driving member driving the second movable seat to move up and down via the second transmission member.

[0016] In some embodiments, the column loading module further comprises a lower end cap mounting and dismounting assembly for cooperating with the carrying mechanism to pull out and reset the lower end cap of the chromatographic column and the sample loading column, and a positioning mechanism for positioning the chromatographic column and the sample loading column.

[0017] In some embodiments, the automatic medium-pressure preparation and separation device further comprises a liquid leakage sensing module for detecting liquid leakage and giving real-time alarm, the liquid leakage sensing module comprising a plurality of liquid leakage sensors, the positions of the liquid leakage sensors comprising the second movable seat and the base for placing the container tray in the fraction collection module.

[0018] In some embodiments, the cleaning column module comprises a placing seat capable of placing a plurality of cleaning columns.

[0019] In some embodiments, the carrying mechanism comprises a mechanical arm and a ground rail module, the mechanical arm moving on the ground rail module, the end of the mechanical arm being provided with a carrying tool, and the carrying tool being detachably connected with the mechanical arm.

[0020] In some embodiments, the mechanical arm is a four-axis mechanical arm, and the carrying tool is a container gripper or a tray gripper.

[0021] In some embodiments, a cover body is arranged on the workbench, an internal accommodation space being formed between the cover body and the workbench, an interaction window is arranged on the cover body on one side where the interaction unit is arranged, for material interaction with the external space; and maintenance windows are arranged on the cover body on the remaining sides, for maintenance of the modules in the internal accommodation space.

[0022] In some embodiments, the interaction window is a sliding window, and the maintenance window is a casement window.

[0023] In the second aspect, the embodiments of the present disclosure provide an automatic separation and preparation system, comprising a mobile device and at least one automatic medium-pressure preparation and separation device as described above, the mobile device being used for conveying materials to the automatic medium-pressure preparation and separation device and taking away samples processed by the automatic medium-pressure preparation and separation device.

[0024] The automatic medium-pressure preparation separation device, the automatic separation preparation system according to the present disclosure can be applied to the traditional Chinese medicine separation scene, and the whole process is realized automatically, the labor cost is reduced, the separation and purification of traditional Chinese medicine components are realized through a chromatographic column, and the following beneficial effects are realized: 1, the automatic liquid suction and column loading process greatly improves the sample processing speed, so that a large number of samples can be processed in a short time; 2, machine operation reduces human error and ensures the consistency of the volume and rate of each liquid suction and column loading, thereby improving the repeatability and accuracy of the experiment and making the experimental work more standardized; 3, the user only needs to set the relevant parameters, and the automatic medium-pressure preparation separation device can automatically complete the whole process, reducing the tedious manual operation; 4, as the automatic equipment is used, the contact between samples can be reduced, and the risk of cross contamination is reduced; 5, the user does not need to directly contact harmful chemicals or biological samples, reducing the potential health risk; 6, the automatic system can accurately control the amount of reagent used, reducing waste, which is particularly important when expensive reagents are used. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 is a top view of an automatic medium-pressure preparation separation device of an embodiment of the present disclosure without a cover;

[0026] Figure 2 is a perspective structural schematic view of an automatic medium-pressure preparation separation device of an embodiment of the present disclosure without a cover;

[0027] Figure 3 is a perspective view of a sample loading and liquid adding and needle cleaning module of an embodiment of the present disclosure;

[0028] Figure 4 is a side view of a sample loading and liquid adding and needle cleaning module of an embodiment of the present disclosure;

[0029] Figure 5 is a schematic view of a needle cleaning part of a sample loading and liquid adding and needle cleaning module of an embodiment of the present disclosure;

[0030] Figure 6 is a perspective structural schematic view of a needle cleaning part of a sample loading and liquid adding and needle cleaning module of an embodiment of the present disclosure;

[0031] Figure 7 is a perspective view of a column loading module of an embodiment of the present disclosure;

[0032] Figure 8 is a rear view of a column loading module of an embodiment of the present disclosure;

[0033] Figure 9 is a perspective view of a column installed in a column loading module of an embodiment of the present disclosure;

[0034] Figure 10is a perspective view of one of the mobile phase buffer tanks in a mobile phase buffer module of an embodiment of the present disclosure;

[0035] Figure 11 is a perspective view of an automated medium pressure preparative separation apparatus with a cover in a state of the present disclosure. DETAILED DESCRIPTION

[0036] For those skilled in the art to better understand the technical solutions of the present disclosure, the technical solutions of the present disclosure will be described in detail below in combination with the drawings.

[0037] In the following, example embodiments will be described more fully with reference to the accompanying drawings, in which example embodiments can be embodied in different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the present disclosure to those skilled in the art.

[0038] In the case of no conflict, each embodiment of the present disclosure and each feature in the embodiments can be combined with each other.

[0039] As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0040] The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present disclosure. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and / or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and / or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and / or groups thereof.

[0041] The embodiments described herein can be described with reference to plan views and / or cross-sectional views by virtue of the idealized nature of the drawings. Thus, the example illustrations are presented for the purpose of simplifying the present disclosure and are not necessarily drawn to scale relative to one another. For the same reason, the dimensions of the different features in the drawings can also have been given relative to one another. Therefore, the embodiments are not limited to the precise drawings illustrated in the figures.

[0042] Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and the present disclosure and will not be interpreted in an overly literal or overly formal sense unless expressly so defined herein.

[0043] Figure 1 is a top view of the automated medium-pressure preparation and separation device without the cover according to an embodiment of the present disclosure, Figure 2 is a perspective view of the automated medium-pressure preparation and separation device without the cover according to an embodiment of the present disclosure. Reference is made to Figure 1 and Figure 2 The automated medium-pressure preparation and separation device 100 includes a workbench 11 on which a plurality of modules are arranged, which will be described in detail below.

[0044] The workbench 11 includes a first side, i.e., a front side 111, a second side, i.e., a rear side 112, a third side, i.e., a left side 113, and a fourth side, i.e., a right side 114. The front side 111 refers to the side close to the user in the natural use state. The rear side 112 is opposite to the front side 111 and refers to the side away from the user in the use state. The left side 113 refers to the left side of the user in the use state. The right side 114 is opposite to the left side 111 and refers to the right side of the user in the use state.

[0045] The interaction unit 1 is arranged near the first side, i.e., the front side 111 of the workbench 11, and is used to interact with external materials. For example, the material containers and / or trays delivered by the mobile device, such as an external AGV trolley, such as a tray containing empty test tubes, a tray containing chromatographic columns, a tray containing sample columns, a tray containing sample test tubes, etc., and the sample containers and / or trays processed in the device are placed in the interaction unit 1 and delivered by the mobile device to other processing systems.

[0046] In some embodiments, the sample is a biological sample, a medical sample, or a chemical sample. When the sample is a medical sample, it can be a Western medicine sample, a traditional Chinese medicine sample, or other drug samples with medicinal effects, etc. For example, the traditional Chinese medicine sample includes traditional Chinese medicinal materials, traditional Chinese medicine decoction pieces, Chinese patent medicines, semi-finished products of traditional Chinese medicines, medicinal plants, etc.

[0047] The interaction unit 1 includes a support plate arranged on the workbench 11, and a storage plate connected to the support plate, which is used to store material containers and / or trays. The storage plate is provided with at least two first storage positions for storing material containers and / or trays. Each first storage position can include a set of positioning members and a sensor. The positioning members can be provided in two, which can be protrusions or grooves, etc. The bottom of the material container and / or tray is correspondingly provided with a mechanism capable of being clamped with the protrusion or groove, so as to limit the material container and / or tray through the positioning members. The positioning members are used to limit the material container and / or tray in the storage position, and the sensor is used to sense whether the storage position stores the material container and / or tray. Optionally, a plurality of layers of storage plates can be arranged at intervals, and at least two first storage positions are arranged on each layer of storage plates. In this way, the interaction unit 1 can have a plurality of first storage positions, and a plurality of storage positions can simultaneously take and place material containers and / or trays, thereby improving efficiency.

[0048] The carrying mechanism 6 is arranged on the second side, i.e., the rear side 112, of the workbench 11 closer to the interaction unit 1, and adjacent to the interaction unit 1. The carrying mechanism 6 is used to transfer materials between modules, and is used to carry various trays and various chromatographic columns and sample columns to various workstations, so as to run the whole machine workstation in series. The carrying mechanism 6 is also used to operate the materials in the modules, such as docking the sample column, the chromatographic column, the washing column, etc. into the module in the column loading module.

[0049] In some embodiments, as shown in Figure 1 and 2 The carrying mechanism 6 includes a mechanical arm 62 and a ground rail module 61. The ground rail module 61 is arranged on the entire length of the workbench 11, and the mechanical arm 62 moves on the ground rail module 61. The ground rail module 61 can adopt a single rail design, a double rail design or any other suitable design. The ground rail module 61 can include only straight rails, or can include straight rails and curved rails for changing the direction of travel of the rails, for example, when the ground rail module is L-shaped or needs to turn. The mechanical arm 62 has components matched with the rails on the ground rail module 61, so that the mechanical arm 62 can move on the ground rail module 61, and can brake and fix when reaching the designated position of the ground rail module 61, so as to facilitate the improvement of the operation stability of the mechanical arm 62.

[0050] The mechanical arm 62 can be a four-axis mechanical arm, and a carrying tool 63 is mounted at the end of the mechanical arm 62. The carrying tool 63 is detachably connected with the mechanical arm 62. The carrying tool 63 can be an electrically operated gripper, and different carrying tools 63 such as a container gripper, a tray gripper, etc. can be switched according to the tray or container to be transferred. A tool warehouse can also be arranged on the workbench 11, and a plurality of carrying tools 63 are stored in the tool warehouse. Alternatively, in an embodiment, a quick-change male head is arranged on the mechanical arm 62, and a quick-change female head is arranged on each carrying tool 63. The quick-change male head and the quick-change female head are matched to achieve detachable connection between the mechanical arm 62 and the carrying tool 63. The quick-change male head and the quick-change female head can be quickly connected in a manner of pneumatic connection, magnetic attraction, etc., which is not limited herein. The mechanical arm 62 drives the carrying tool 63 to move and clamp the corresponding container or tray and transfer it to the target position.

[0051] Alternatively, at least one storage rack 2 can also be arranged on the workbench 11. Figure 1 and Figure 2 In the embodiment shown, three storage racks 2 are arranged on the workbench 11. One of the storage racks 2 is arranged integrally with the interactive unit 1, and another storage rack 2 is arranged side by side with the storage rack. A storage rack 2 is also arranged at a position closer to the inner side of the workbench 11 than the positions of the two storage racks 2.

[0052] The structure of the storage rack 2 can be similar to that of the interactive unit 1, which includes a support plate arranged on the workbench 11 and a storage plate connected to the support plate. The storage plate is used to store material containers and / or trays. The storage plate is provided with at least two second storage positions for storing material containers and / or trays. Each second storage position can include a set of positioning members and a sensor. The positioning members can be two, which can be protrusions or grooves, and the bottom of the material container and / or tray is correspondingly provided with a mechanism capable of being clamped with the protrusions or grooves, so as to limit the material container and / or tray by the positioning members. The positioning members are used to limit the material container and / or tray in the storage position, and the sensor is used to sense whether the storage position stores the material container and / or tray. Alternatively, a plurality of layers of storage plates can be arranged at intervals, and at least two second storage positions are arranged on each layer of storage plates. In this way, the storage rack 2 can have a plurality of second storage positions, and a plurality of storage positions can simultaneously take and place material containers and / or trays, which can improve the efficiency.

[0053] When the mobile device transfers more material containers and / or trays, the interactive unit 1 cannot accommodate them at one time, and the material containers and / or trays can be temporarily stored on the storage rack 2. For example, in the preparation stage of the experiment, the mobile device transfers various materials required for the experiment, such as trays containing empty test tubes, trays containing chromatographic columns, trays containing sample columns, trays containing sample test tubes, etc., to the interactive unit 1, and the handling mechanism 6 transfers the various trays on the interactive unit 1 to the storage rack 2. During the experiment, the samples processed by each module can also be temporarily stored on the storage rack 2, and then transferred to the next module by the handling mechanism 6. After all the processing of the samples is completed, the handling mechanism 6 transfers the containers and / or trays on the storage rack 2 to the interactive unit 1, and the mobile device transports them to other processing devices.

[0054] On the side of the second side, i.e., the rear side 112, of the workbench 11 closer to the handling mechanism 6, the sample loading and liquid adding module 3, the column loading module 4, the column cleaning module 5, the fraction collection module 8, the pump and valve module 10, the controller 12, Figure 1 and 2 In the embodiment shown, the sample loading and liquid adding module 3, the fraction collection module 8, and the pump and valve module 10 are each provided with two, but the number is not limited, and the number of these modules can be set according to actual processing needs. The mobile phase buffer module 9 includes a plurality of mobile phase buffer tanks, which can be independently provided on the workbench 11, and in Figure 1 and Figure 2 In the embodiment shown, the mobile phase buffer module 9 is provided on the side of the second side, i.e., the rear side, of the workbench 11, and the bottom of the mobile phase buffer module 9 can be provided with a plurality of rollers, which can be one-way or universal rollers, and can roll on the ground to facilitate the movement of the mobile phase buffer module 9. An upper plug placement module 7 is also provided on one side of the ground rail module 61.

[0055] The controller 12 is used to control the action of the transport mechanism 6 and the operating procedures of each module to realize the processing automation of the automated medium pressure preparation and separation equipment 100. The controller 12 can be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field programmable gate arrays (FPGA) or other programmable logic devices, discrete gates or transistor logic devices, discrete hardware components, etc. The general-purpose processor can be a microprocessor or the processor can also be any conventional processor, etc. The controller 12 can be set in the automated medium pressure preparation and separation equipment 100, or it can be set in the cloud or separated from the automated medium pressure preparation and separation equipment 100. When the controller 12 is separated from the automated medium pressure preparation and separation equipment 100, the controller interacts with the automated medium pressure preparation and separation equipment 100 through the network to convey various control instructions and transmit data. The network may be the Internet, a virtual private network (VPN), a wireless network including WIFI and Bluetooth, a telephone network including GPRS and CDMA networks, and a radio and television network. Figure 1 and Figure 2 In the illustrated embodiment, the controller 12 is disposed on the workbench 11 , and each module may be electrically connected to the controller 12 or connected via a network.

[0056] The sample loading and liquid addition and needle cleaning module 3 is used for loading and aspirating sample liquid, and also supports cleaning of the sample loading needle. Figure 3 This is a three-dimensional diagram of a sample loading, liquid addition and needle cleaning module according to an embodiment of the present disclosure. Figure 4 2 is a side view of a sample loading, liquid addition and needle cleaning module according to an embodiment of the present disclosure.

[0057] like Figure 3 and Figure 4As shown, the sample loading liquid adding part includes a lifting mechanism 31, a sample loading needle mounting seat 32 arranged on the lifting mechanism 31, a sample loading needle 33 mounted in the sample loading needle mounting seat 32, a plunger pump not shown in the figure in communication with the sample loading needle 33, a container seat 34 below the sample loading needle 33, and a translation mechanism 35 below the container seat 34. The lifting mechanism 31 can drive the sample loading needle mounting seat 32 to lift, so as to drive the sample loading needle 33 to move in the up-down direction. The container seat 34 can be used to place a container 37, such as a test tube or a concentrate bottle containing sample liquid. The translation mechanism 35 can drive the container seat 34 to move horizontally, so as to drive the container 37 to move in the horizontal direction. The sample loading needle 33 is also in communication with the pump valve module 10, and the plunger pump is used to drive the sample loading needle 33 to suck sample liquid from the container 37 and introduce it into the pump valve module 10.

[0058] Figure 5 is a schematic view of a sample loading needle cleaning part of an embodiment of the present disclosure, Figure 6 is a schematic view of a sample loading needle cleaning part of an embodiment of the present disclosure. As shown in Figure 5 and Figure 6 As shown, the sample loading needle cleaning part includes a cleaning seat 36 arranged on the translation mechanism 35 and a waste liquid bottle not shown in the figure in communication with the cleaning seat 36. The cleaning seat 36 is in the form of a rectangular block structure, and the inside of the cleaning seat 36 is provided with a first cleaning channel 361 for cleaning the outer wall of the sample loading needle 33, a second cleaning channel 362 for cleaning the inner wall of the sample loading needle 33, and a liquid discharge channel 363 for discharging waste liquid. The first cleaning channel 361 and the second cleaning channel 362 are arranged separately and adjacent to each other. One end of the first cleaning channel 361 has a first inlet 364 for the sample loading needle 33 to insert, and the other end is closed. One end of the second cleaning channel 362 has a second inlet 365 for the sample loading needle 33 to insert, and the other end is in communication with the liquid discharge channel 363. The first inlet 364 and the second inlet 365 are in communication, and the liquid discharge channel 363 has a liquid discharge port 366 in communication with the waste liquid bottle not shown in the figure.

[0059] When cleaning the inner wall of the sample loading needle 33, the sample loading needle 33 is inserted into the second cleaning channel 362, and the cleaning liquid is sprayed out of the sample loading needle 33 to complete the cleaning of the inner wall, and the waste liquid after cleaning is discharged out of the module through the liquid discharge port 366. When cleaning the outer wall of the sample loading needle 33, the sample loading needle 33 is inserted into the first cleaning channel 361, and the cleaning liquid is sprayed out of the sample loading needle 33, the cleaning liquid accumulates in the first cleaning channel 361, the liquid level gradually rises, and then the cleaning liquid overflows the first cleaning channel 361 to complete the cleaning of the outer wall, and the waste liquid after cleaning reaches the second cleaning channel 362 through the overflow, and is finally discharged through the liquid discharge port 366. The flow direction of the cleaning liquid is shown by the arrow in Figure 5 .

[0060] That is, there is no outlet at the bottom of the outer wall cleaning channel of the sample loading needle 33, and the cleaning liquid flowing out of the sample loading needle 33 is accumulated in this channel. The accumulated cleaning liquid can clean the outer wall of the sample loading needle 33. The inlet of the inner wall cleaning channel of the sample loading needle 33 is connected to the inlet of the outer wall cleaning channel of the sample loading needle 33. The inner wall cleaning channel of the sample loading needle 33 is provided with a drain port, which is connected to a waste liquid bottle. The cleaning liquid in the outer wall cleaning channel of the sample loading needle 33 can overflow into the inner wall cleaning channel and be discharged from the drain port into the waste liquid bottle. When the inner and outer walls of the sample loading needle 33 are cleaned, the cleaning liquid can be stored in the mobile phase buffer module 9, and the pump valve module 10 draws the cleaning liquid into the sample loading needle 33 for outflow.

[0061] The column packing module 4 is used for docking chromatographic columns and sample loading columns of various specifications and sizes, eluting samples in the chromatographic columns and sample loading columns, and cooperating with the transport mechanism 6 to realize the extraction of the lower plugs of the chromatographic columns and sample loading columns. Figure 7 is a perspective view of a column packing module according to an embodiment of the present disclosure; Figure 8 is a rear view of a column packing module according to an embodiment of the present disclosure; Figure 9 FIG1 is a perspective view of a column installation module according to an embodiment of the present disclosure, in which columns are installed.

[0062] Reference Figure 7 to Figure 9 The column mounting module 4 mainly includes a first movable seat 401, a second movable seat 402, a fixed seat 403, a first driving mechanism 420 for driving the first movable seat 401 to rise and fall, and a second driving mechanism 410 for driving the second movable seat 402 to rise and fall. The first movable seat 401 and the second movable seat 402 are arranged on one side of the vertical plate 407, with the first movable seat 401 located above and opposite to the second movable seat 402. The first driving mechanism 420 and the second driving mechanism 410 are arranged on the other side of the vertical plate 407 and on the base 406. The fixed seat 403 is arranged on the base 406, with the fixed seat 403 located below and opposite to the second movable seat 402. The base 406 is also provided with a first lower plug loading and unloading mechanism 404 and a positioning mechanism 405. A second lower plug loading and unloading mechanism 408 is provided on the vertical plate 407 on one side of the second movable seat 402.

[0063] The first movable seat 401 is provided with a plurality of first joints, the second movable seat 402 is provided with a plurality of second joints, and the fixed seat 403 is provided with a plurality of third joints. The sample column 430 is arranged between the first movable seat 401 and the second movable seat 402, and the chromatographic column 440 is arranged between the second movable seat 402 and the fixed seat 403 through the carrying mechanism 6. Of course, the positions of the sample column 430 and the chromatographic column 440 can also be exchanged according to needs. The liquid supply module, the sample column 430, the chromatographic column 440, and the liquid receiving module can be connected through the first movable seat 401, the second movable seat 402, and the fixed seat 403. The liquid supply module can correspond to the sample liquid feeding module and the needle cleaning module 3 and the mobile phase buffer module 9 connected through the pump valve module 10, and the liquid receiving module can correspond to the fraction collection module 8 connected through the pump valve module 10.

[0064] One end of the first joint on the first movable seat 401 can be connected with the liquid supply module, and the other end can be connected with the liquid inlet of the sample column 430. One end of the second joint on the second movable seat 402 can be connected with the liquid outlet of the sample column 430 or the liquid supply module, and the other end can be connected with the liquid inlet of the chromatographic column 440. One end of the third joint on the fixed seat 403 can be connected with the liquid outlet of the chromatographic column 440, and the other end can be connected with the liquid receiving module. The specifications of the plurality of third joints can be the same or different, wherein the difference in specifications can be that the diameters of the plurality of third joints are different, so as to be applicable to chromatographic columns 440 with different liquid outlet diameters. Of course, the plurality of third joints with different specifications can improve the compatibility of the column loading module 4, and the plurality of third joints with the same specification can improve the experimental throughput. The same is true for the plurality of first joints and the plurality of second joints.

[0065] The first driving mechanism 420 can include a first transmission member 421 and a first driving member 422, wherein the first transmission member 421 connects the first driving member 422 and the first movable seat 401. The first driving member 422 includes but is not limited to a pneumatic cylinder or an electric motor. The first driving member 422 can provide power for the lifting of the first movable seat 401 to drive the first movable seat 401 to approach or move away from the second movable seat 402. Similarly, the second driving mechanism 410 can include a second transmission member 412 and a second driving member 411, wherein the second transmission member 412 connects the second driving member 411 and the second movable seat 402. The second driving member 411 includes but is not limited to a pneumatic cylinder or an electric motor. The second driving member 411 can provide power for the lifting of the second movable seat 402 to drive the second movable seat 402 to approach or move away from the fixed seat 403.

[0066] The first lower plug mounting and dismounting mechanism 404 is used to pull out or mount the lower plug of the liquid outlet of the column, such as the chromatographic column 440, which is arranged between the second movable seat 402 and the fixed seat 403. Before the column mounting operation, the liquid outlet of the chromatographic column 440 is blocked by the lower plug arranged at the liquid outlet, so as to avoid the outflow of the substances in the chromatographic column 440. After the lower plug at the liquid outlet of the chromatographic column 440 is pulled out by the first lower plug mounting and dismounting mechanism 404, the liquid inlet and the liquid outlet of the chromatographic column 440 are in communication with the outside. The first lower plug mounting and dismounting mechanism 404 is provided with a fixing member and a clamping groove. The lower plug at the liquid outlet of the chromatographic column 440 is arranged in the clamping groove, and then the chromatographic column 440 is moved upwards, so as to pull out the lower plug. The second lower plug mounting and dismounting mechanism 408 is used to pull out or mount the lower plug of the liquid outlet of the column, such as the sample loading column 430, which is arranged between the first movable seat 401 and the second movable seat 401. By arranging the second lower plug mounting and dismounting mechanism 408 on the side of the first movable seat 401, the plug pulling operation can be completed in the fixed position of the sample loading column 430, so as to avoid the long-distance movement of the sample loading column 430 and the resulting liquid drop, thereby improving the speed of sample processing.

[0067] The positioning mechanism 405 is used to position the chromatographic column 440 and / or the sample loading column 430. The positioning mechanism 405 can be a positioning camera, which is used for visual positioning to determine the accurate position of the chromatographic column 440 and the sample loading column 430. Thus, after the lower plug of the chromatographic column 440 and the sample loading column 430 is pulled out, the liquid outlet of the chromatographic column 440 and the sample loading column 430 can be quickly positioned by the positioning mechanism 405, so as to accurately connect the liquid outlet with the connectors on the first movable seat 401, the second movable seat 402 and the fixed seat 403, thereby improving the success rate of connection.

[0068] In the dry sample loading scenario, one end of the first connector on the first movable seat 401 is connected with the liquid supply module, and the other end is connected with the liquid inlet of the sample loading column 430. One end of the second connector on the second movable seat 402 is connected with the liquid outlet of the sample loading column 430, that is, the sample loading column 430 is connected between the first movable seat 401 and the second movable seat 402. The other end of the second connector on the second movable seat 402 is connected with the liquid inlet of the chromatographic column 440. One end of the third connector on the fixed seat 403 is connected with the liquid outlet of the chromatographic column 440, that is, the chromatographic column 440 is connected between the second movable seat 402 and the fixed seat 403. The other end of the third connector on the fixed seat 403 is connected with the liquid receiving mechanism. The liquid supply module is used to supply liquid, such as eluent, into the sample loading column 430. After the liquid is mixed with the sample in the sample loading column 430, the mixture flows into the chromatographic column 440 through the first movable seat 401 to separate the components. The separated components flow into the liquid receiving module through the fixed seat 403, so as to realize the separation and purification of the sample components.

[0069] In a wet sample loading scenario, one end of the first connector on the first movable seat 401 is connected to the liquid supply module, and the other end is connected to the liquid inlet of the chromatographic column 440. One end of the second connector on the second movable seat 402 is connected to the liquid outlet of the chromatographic column 440, that is, the chromatographic column 440 is connected between the first movable seat 401 and the second movable seat 402. The other end of the second connector on the second movable seat 402 is connected to the liquid inlet of the cleaning column, and one end of the third connector on the fixed seat 403 is connected to the liquid outlet of the cleaning column, that is, the cleaning column is connected between the second movable seat 402 and the fixed seat 403. The other end of the third connector on the fixed seat 403 is connected to the liquid receiving mechanism. In addition, the chromatographic column 440 and the cleaning column can also be swapped. The cleaning column here serves as a connecting pipe. Liquid, such as sample and eluent, is supplied to the chromatographic column 440 through the liquid supply module and flows into the chromatographic column 4000 for component separation. The separated components flow from the fixed seat 403 into the liquid receiving module to achieve separation and purification of the sample components.

[0070] When docking the sample loading column 430, the chromatography column 440, the cleaning column and other columns, the first movable seat 401 and the second movable seat 402 can be driven up and down by the first driving mechanism 420 and the second driving mechanism 410 according to the height of the column to adjust the distance between the first movable seat 401, the second movable seat 402 and the fixed seat 403 to ensure that columns of different heights can be matched and installed.

[0071] In summary, multiple joints are provided on the first movable seat 401, the second movable seat 402, and the fixed seat 403, and the spacing between the first movable seat 401, the second movable seat 402, and the fixed seat 403 can be adjusted by a drive mechanism, thereby allowing the installation of multiple columns of the same or different specifications. While conventional processes require manual loading of columns, the automated medium-pressure preparative separation apparatus disclosed herein enables automated operation by directly loading and unloading columns into and from the column loading module 4 using a robotic arm.

[0072] The column packing module 4 disclosed herein not only improves the automation level of the column packing operation and reduces labor costs, but also enhances the module's compatibility. The automated column packing process significantly increases the speed of sample processing, enabling the processing of large numbers of samples in a shorter period of time, saving time and reducing human error, thereby improving the repeatability and accuracy of experiments, enhancing the efficiency, accuracy, and safety of experiments, and making laboratory work more efficient and standardized.

[0073] The cleaning column module 5 is arranged on one side of the column loading module 4, and is used for buffering and placing a plurality of cleaning columns. The cleaning column module 5 can be regarded as a placing seat, which can place a plurality of cleaning columns, for example, four cleaning columns. The cleaning columns are used in cooperation with the column loading module 4. When the column loading module 4 needs to be cleaned, the conveying mechanism 6 takes out the cleaning column from the placing seat and installs the cleaning column in the column loading module 4, so as to clean the pipeline or the chromatographic column / sample column of the column loading module 4. In addition, the cleaning column can also be used as a connecting pipeline when the chromatographic column / sample column is independently loaded and eluted.

[0074] The upper plug placing module 7 is used for buffering and placing upper plugs of various chromatographic columns and sample columns. The conveying mechanism 6 can place the upper plug taken out from the chromatographic column and the sample column in the module, and take the upper plug out of the module to reinstall it.

[0075] The fraction collection module 8 is used for buffering different containers compatible with a plurality of different trays, and is used for collecting fractions condensed at different time periods or at different temperatures, so as to facilitate subsequent analysis or use.

[0076] The mobile phase buffering module 9 is used for buffering a large amount of mobile phase liquid. The mobile phase buffering module 9 can include a plurality of mobile phase buffering tanks, Figure 10 is a perspective view of a mobile phase buffering tank. As shown in Figure 10 , the mobile phase buffering tank is provided with a turnover door 901 on the tank shell. A large-capacity barrel containing mobile phase, such as a 30L large-capacity barrel, is placed inside. When the liquid in the barrel is insufficient, the turnover door 901 is manually opened to take out the barrel inside to add liquid. The barrel inside is connected to the pump valve module 10 through the through hole 902 on the tank shell through a pipeline. In the prior art, the structure of the mobile phase buffering module is small in capacity, and manual liquid addition is required frequently. The bottom of the mobile phase buffering tank is provided with a plurality of rollers 903, which can be one-way or universal rollers, and can roll on the ground to facilitate the movement of the mobile phase buffering module 9. The mobile phase stored in different buffering tanks can be different.

[0077] The mobile phase buffering module 9 can include any number of buffering tanks, so as to store a large amount of mobile phase liquid, so as to ensure that the required mobile phase liquid can be continuously supplied without interruption for a long time experiment of 24 hours, 48 hours, etc., without interrupting the experiment process to supplement the liquid. The number of buffering tanks in the mobile phase buffering module 9 is not limited, as long as the experimental requirements can be met.

[0078] The pump valve module 10 is connected to the sample liquid adding and needle cleaning module 3, the column loading module 4, the fraction collection module 8, and the mobile phase buffering module 9 through pipelines, and provides power for the liquid transmission between the above modules.

[0079] The automatic medium-pressure preparation and separation device provided by the embodiments of the present disclosure is further provided with a liquid leakage sensing module. The liquid leakage sensing module comprises liquid leakage sensors arranged at a plurality of detection positions, and the liquid leakage sensors are connected with the controller 12. The liquid leakage sensors can be arranged on the base of the fraction collection module 8 for placing the container tray. When the fraction collection is performed, if no container is placed or the liquid in the container overflows, the liquid leakage sensors can sense the liquid leakage, and the controller 12 can be timely informed to stop the pump valve module 10 from pumping liquid. The liquid leakage sensors can also be arranged on the second movable seat 402 of the column loading module 4. When the column loading operation is performed, if the liquid leakage occurs at the joint between the liquid outlet of the sample column and the joint, the liquid leakage sensors can sense the liquid leakage, and the controller 12 can be timely informed to stop the pump valve module 10 from pumping liquid. The liquid leakage sensors can also be arranged at other positions where liquid leakage needs to be detected, to perform liquid leakage detection and real-time alarm, thereby ensuring the reliability of the results when the system is operated unattended.

[0080] Figure 11 FIG. 1 is a perspective view of an automatic medium-pressure preparation and separation device with a cover according to an embodiment of the present disclosure. In some embodiments, the automatic medium-pressure preparation and separation device 100 can comprise a cover 200 arranged on a workbench 11. The cover 200 and the workbench 11 form an internal accommodation space therebetween, and the modules described above are located in the accommodation space. Optionally, the cover 200 comprises a frame 600 arranged on the workbench 11. The workbench 11 and the frame 600 can be detachably connected or integrally formed, which is not limited herein. In some embodiments, only the workbench 11 can be provided without the frame 600.

[0081] The internal accommodation space is defined by the frame structure of the workbench 11 and the frame 600, i.e., the upper surface of the workbench 11 and the enclosure of the frame 600 form the internal accommodation space. Optionally, the interior of the workbench 11 also has an accommodation space. In the case of multiple sub-frames of the cover 200, each sub-frame can have its own sub-space. In the multiple sub-spaces, some sub-spaces can be interconnected, and some sub-spaces can be relatively independent and not connected with other sub-spaces, which is not limited herein.

[0082] The frames 600 are connected by plate-shaped structures to form the cover 200. The material of the cover 200 can be steel, aluminum alloy, etc., which is not limited herein. The overall shape of the cover 200 can be a cuboid or any other feasible shape, which is not limited herein. The cover 200 can comprise a plurality of support vertical beams, support horizontal beams, etc., to form a frame structure.

[0083] The interactive windows 300 and 301 are arranged on the front side of the cover 200, and the interactive windows 300 and 301 communicate the internal accommodation space and the external space. Figure 9The interaction window 300 is shown in an open state, and the interaction window 301 is shown in a closed state. In the open state of the interaction window 300, the interaction unit 1 can interact with the outside. The mobile device moves to the interaction window 300, places the material container and / or tray delivered to the interaction unit 1, or takes away the sample container and / or tray placed on the interaction unit 1 after processing. Since the interaction window 300 is mainly used for the interaction between the internal accommodation space and the external space, in order to facilitate the interaction, the interaction window 300 is arranged in the form of a movable sliding window, which can be temporarily fixed when moved upward to the open state, keeping the window open and not affecting the interaction between the internal accommodation space and the external space.

[0084] In Figure 11 The right side of the automated medium-pressure preparation and separation device 100, i.e., the right side of the workbench 1, is provided with a maintenance window 400 on the cover 200, which is mainly used for the maintenance of the modules in the internal accommodation space by the workers. In order to facilitate the operation of the workers, the maintenance window 400 is arranged in the form of a flat window, so that more internal accommodation space can be exposed when the maintenance window 400 is opened, facilitating the maintenance of the workers.

[0085] A duct assembly 500 is further arranged on the top of the cover 200, which is used to control the working temperature of the internal accommodation space and replace the air.

[0086] Optionally, in an embodiment of the present disclosure, an automated separation and preparation system is also provided, which comprises a mobile device and at least one automated medium-pressure preparation and separation device. The mobile device is used to deliver materials to the automated medium-pressure preparation and separation device, and take away samples processed by the automated medium-pressure preparation and separation device.

[0087] For example, in the preparation stage of the experiment, the mobile device transfers various materials required for the experiment, such as a tray containing empty test tubes, a tray containing chromatographic columns, a tray containing sample columns, a tray containing sample test tubes, etc., to the interaction unit 1 of the automated medium-pressure preparation and separation device 100; the products of the preparation and separation steps can be transferred to the interaction unit 1 by the carrying mechanism 6, and then delivered to other processes by the mobile device. The mobile device can be any mobile device capable of achieving the above functions in the prior art, such as but not limited to a mobile AGV trolley or a mobile robot.

[0088] Example embodiments have been disclosed herein and, although specific terms are employed, they are used in a generic and descriptive sense only and not for purposes of limitation. In some instances, it will be apparent to those skilled in the art that features, characteristics or / and elements described in connection with a particular embodiment can be used in conjunction with other embodiments unless otherwise explicitly stated. Accordingly, it will be understood that various changes in form and details can be made without departing from the scope of the disclosure as set forth in the appended claims.

Claims

1. An automated medium-pressure preparation and separation equipment, characterized in that: include: Workbench; The sample loading and liquid addition and needle cleaning module, column packing module, fraction collection module, transport mechanism and pump valve module are arranged on the workbench; and a mobile phase buffer module independently provided on the workbench, wherein: The sample addition and needle cleaning module is used to load and absorb the sample liquid and clean the sample needle; A column packing module, wherein the chromatographic column and / or the sample loading column is installed in the column packing module through the transport mechanism to separate the components of the sample. The column packing module can be provided with chromatographic columns and / or sample loading columns of various specifications; a fraction collection module, for collecting fractions; A transport mechanism for transferring materials between the sample loading and liquid addition and needle cleaning module, the column packing module and the fraction collection module; A mobile phase buffer module, used for buffering and storing required mobile phase; and The pump and valve module is respectively connected with the sample loading and liquid adding and needle cleaning module, the column packing module, the fraction collection module, and the mobile phase buffer module to provide power for liquid transmission between the modules.

2. The automated medium-pressure preparation and separation equipment according to claim 1, characterized in that: The automated medium-pressure preparation and separation equipment further comprises: An interaction unit, which interacts with the outside world through the transport mechanism; A cleaning column module, used for caching and placing the cleaning column; and The liquid leakage sensing module is used to detect liquid leakage and issue an alarm in real time.

3. The automated medium-pressure preparation and separation equipment according to claim 1, characterized in that: The automated medium-pressure preparation and separation equipment further comprises: Storage racks for placing materials required for processing in each module; and The upper plug placement module is used for caching and placing the upper plugs of the chromatographic column and / or the sample loading column.

4. The automated medium-pressure preparation and separation equipment according to claim 1, characterized in that: The mobile phase buffer module includes a plurality of mobile phase buffer boxes, in which large-capacity barrels filled with mobile phase are placed.

5. The automated medium-pressure preparation and separation equipment according to claim 2, characterized in that: The interaction unit is arranged on a side of the workbench close to the user operation. The sample loading and liquid adding and needle cleaning module, column packing module, and fraction collecting module are sequentially arranged at positions adjacent to the transport mechanism.

6. The automated medium-pressure preparation and separation equipment according to claim 1, characterized in that: The sample loading and liquid adding and needle cleaning module includes a sample loading and liquid adding part and a sample loading needle cleaning part.

7. The automated medium-pressure preparation and separation equipment according to claim 6, characterized in that: The sample loading and liquid adding part includes a lifting mechanism, a sample loading needle arranged on the lifting mechanism, a pump connected to the sample loading needle, a container seat located below the sample loading needle, and a translation mechanism capable of driving the container seat to move, and a container containing the sample liquid is placed in the container seat.

8. The automated medium-pressure preparation and separation equipment according to claim 6, characterized in that: The sample loading needle cleaning part includes a cleaning seat, and a first cleaning channel, a second cleaning channel and a drainage channel arranged in the cleaning seat. The first cleaning channel is used to clean the outer wall of the sample loading needle, the second cleaning channel is used to clean the inner wall of the sample loading needle, and the drainage channel is used to discharge the waste liquid after cleaning. The first cleaning channel and the second cleaning channel are separated from each other, the second cleaning channel is communicated with the drainage channel, and the first cleaning channel and the second cleaning channel share the drainage channel.

9. The automated medium-pressure preparation and separation equipment according to claim 8, characterized in that: One end of the first cleaning channel has a first inlet for the sample loading needle to extend into, and the other end is closed. One end of the second cleaning channel has a second inlet for the loading needle to extend into, and the other end is connected to the drainage channel. The first inlet is connected to the second inlet, The liquid discharge channel has a liquid discharge port, and the liquid discharge port is communicated with a waste liquid bottle.

10. The automated medium-pressure preparation and separation equipment according to claim 1, characterized in that: The column mounting module includes a first movable seat, a second movable seat, and a fixed seat arranged in sequence up and down; a first driving mechanism for driving the first movable seat to rise and fall; and a second driving mechanism for driving the second movable seat to rise and fall. The first movable seat is opposite to the second movable seat, and the second movable seat is opposite to the fixed seat. The first movable seat is provided with a plurality of first joints, the second movable seat is provided with a plurality of second joints, and the fixed seat is provided with a plurality of third joints. The specifications of the multiple first connectors are the same or different, the specifications of the multiple second connectors are the same or different, and the specifications of the multiple third connectors are the same or different.

11. The automated medium-pressure preparation and separation equipment according to claim 10, characterized in that: One end of the first connector is connected to the sample loading and needle cleaning module and the mobile phase buffer module through the pump valve module, and one end of the third connector is connected to the fraction collection module through the pump valve module.

12. The automated medium-pressure preparation and separation equipment according to claim 10, characterized in that: The first driving mechanism includes a first transmission member and a first driving member, wherein the first transmission member connects the first driving member and the first movable seat, and the first driving member drives the first movable seat to move up and down via the first transmission member. The second driving mechanism includes a second transmission member and a second driving member, the second transmission member connects the second driving member and the second movable seat, and the second driving member drives the second movable seat to move up and down via the second transmission member.

13. The automated medium-pressure preparation and separation equipment according to claim 10, characterized in that: The column packing module further comprises: The lower plug assembly and disassembly assembly is used to cooperate with the transport mechanism to remove and replace the lower plugs of the chromatographic column and the loading column, and the positioning mechanism is used to position the chromatographic column and the loading column.

14. The automated medium-pressure preparation and separation equipment according to claim 10, characterized in that: The automated medium-pressure preparation and separation equipment also includes a liquid leakage sensing module for detecting liquid leakage and issuing a real-time alarm. The liquid leakage sensing module includes a plurality of liquid leakage sensors, and the positions where the liquid leakage sensors are arranged include on the second movable seat and on the base for placing the container tray in the fraction collection module.

15. The automated medium-pressure preparation and separation equipment according to claim 2, characterized in that: The cleaning column module includes a placement seat capable of placing multiple cleaning columns.

16. The automated medium-pressure preparation and separation equipment according to claim 1, characterized in that: The transport mechanism includes a robotic arm and a ground rail module. The robotic arm moves on the ground rail module. A transport tool is installed at the end of the robotic arm. The transport tool is detachably connected to the robotic arm.

17. The automated medium-pressure preparation and separation equipment according to claim 2, characterized in that: A cover is provided on the workbench, and an internal accommodation space is formed between the cover and the workbench. On one side where the interaction unit is set, an interaction window is set on the cover body for interacting with materials with the external space; on the other sides of the cover body, maintenance windows are set on the cover body for maintaining the modules in the internal accommodation space.

18. An automated separation and preparation system, characterized in that: It comprises a mobile device and at least one automated medium-pressure preparation and separation device according to any one of claims 1 to 17, wherein the mobile device is used to transport materials to the automated medium-pressure preparation and separation device and to take away samples processed by the automated medium-pressure preparation and separation device.

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