Efficient plasma exosome filtering device
By using a combination of silica gel column chromatography and filter paper layer in the plasma exosome filtration device, the problem of poor filtration effect in the existing technology is solved, efficient separation and extraction of plasma exosomes is achieved, and the operation process is simplified.
Patent Information
- Application Number
- CN202422632263.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-30
- Publication Date
- 2025-10-17
- Estimated Expiration
- 2034-10-30
AI Technical Summary
Existing plasma exosome filtration methods have poor filtration effects, making it difficult to efficiently separate and extract exosomes from plasma.
A high-efficiency plasma exosome filtration device was designed, comprising a first cylinder and a second cylinder. A vertical tube was provided in the second cylinder, and silica gel was filled between the outer and inner walls of the vertical tube. Combined with column chromatography and filter paper layers, it was used to adsorb and filter exosomes in plasma, and the exosomes were separated through a separatory funnel.
The filtration effect of plasma exosomes is improved, the separation efficiency is enhanced, the pre-treatment process is simplified, and the use is convenient.
Smart Images
Figure CN223439368U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the technical field of plasma exosome detection, especially relates to a high -efficient plasma exosome filter device. BACKGROUND
[0002] Plasma exosomes are small vesicles secreted by cells, typically ranging in diameter from 30 to 150 nanometers, and are widely present in body fluids such as plasma, urine, and saliva. They contain a variety of biological molecules such as proteins, lipids, and RNA, and can transmit information between cells, participate in cell communication and regulation. Exosomes have attracted widespread attention in biomedical research because they play an important role in the occurrence and development of diseases. For example, exosomes may be related to tumors, neurodegenerative diseases, and cardiovascular diseases. Therefore, studying the composition and function of plasma exosomes helps to understand the disease mechanism and may provide new methods for early diagnosis and treatment.
[0003] Before plasma exosome detection, pretreatment is required, and filtration is an important step in pretreatment. The existing filtration method is mostly simple filtration by filter paper, and the filtration effect is poor. UTILITY MODEL CONTENT
[0004] The utility model aims at providing a kind of high -efficient plasma exosome filter device to solve the problems in the above background technology.
[0005] To achieve the above object, the utility model provides the following technical scheme: a kind of high -efficient plasma exosome filter device, including first cylinder, the inside of the first cylinder is equipped with second cylinder, the inside of the second cylinder is equipped with vertical tube, the cavity is formed between the outer wall of vertical tube and the inner wall of second cylinder, the inside of the cavity is filled with silica gel, the both ends of vertical tube are through setting, and the bottom of vertical tube penetrates the bottom surface of second cylinder, the bottom side of the first cylinder is equipped with outlet pipe, the lateral wall of vertical tube is equipped with through hole, the inside of vertical tube is equipped with filter paper layer, the filter paper layer is attached to the inner wall of vertical tube, and seals through hole.
[0006] Preferably, the upper portion of the first cylinder is equipped with a separatory funnel, the outlet of the separatory funnel is connected with a rubber tube, and the rubber tube is in communication with the cavity between the outer wall of the vertical tube and the inner wall of the second cylinder.
[0007] Preferably, one side of the first cylinder is equipped with a bracket, the top of the bracket is equipped with a fixing clamp, and the fixing clamp clamps the separatory funnel.
[0008] Preferably, the top surface of the first cylinder is equipped with a cylinder cover, the cylinder cover is provided with a connecting pipe, the bottom of the connecting pipe extends into the cavity between the outer wall of the vertical tube and the inner wall of the second cylinder, and the top of the connecting pipe is connected with the rubber tube of the outlet of the separatory funnel.
[0009] Preferably, the bottom surface of the barrel cover is sleeved on the second barrel body and is threadedly connected.
[0010] The utility model at least has the following beneficial effects:
[0011] The utility model provides a kind of efficient plasma exosome filter device, vertical pipe is arranged in the inside of second barrel body, silica gel is filled in cavity, column chromatography is combined with filtration, and filtration effect is improved, in addition, according to requirement, separating funnel is set in the top of first barrel body, further improve the effect of pretreatment, greatly facilitate use. BRIEF DESCRIPTION OF DRAWINGS
[0012] Figure 1 It is internal structure schematic view of the utility model;
[0013] Figure 2 It is external structure schematic view of the utility model.
[0014] In the figure mark: 1, first barrel body;2, second barrel body;3, vertical pipe;4, through hole;5, filter paper layer;6, water outlet pipe;7, connecting pipe;8, separating funnel;9, support. DETAILED DESCRIPTION
[0015] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.
[0016] EMBODIMENT
[0017] Please refer to Figure 1 And Figure 2 The utility model provides a kind of technical schemes: a kind of efficient plasma exosome filter device, including first barrel body 1, the inside of first barrel body 1 is equipped with second barrel body 2, the inside of second barrel body 2 is equipped with vertical pipe 3, and the cavity is formed between the outer wall of vertical pipe 3 and the inner wall of second barrel body 2, and the inside of cavity is filled with silica gel, and the both ends of vertical pipe 3 are through setting, and the bottom of vertical pipe 3 penetrates the bottom surface of second barrel body 2, specifically, vertical pipe 3 is fixedly connected with second barrel body 2, and the bottom of first barrel body 1 is equipped with water outlet pipe 6, and the lateral wall of vertical pipe 3 is equipped with through hole 4, and the inside of vertical pipe 3 is equipped with filter paper layer 5, and filter paper layer 5 is attached to the inner wall of vertical pipe 3, and sealing through hole 4.
[0018] In the embodiment, the cavity between the outer wall of the vertical pipe 3 and the inner wall of the second cylinder 2 is wetted by the reagent in advance, the mixed solution of plasma exosome is introduced into the cavity between the outer wall of the vertical pipe 3 and the inner wall of the second cylinder 2, and since the cavity is filled with silica gel, the silica gel can play the role of adsorption and column chromatography, the mixed solution enters the vertical pipe 3 through the through hole 4, is filtered through the filter paper layer 5, and then enters the first cylinder 1 through the vertical pipe 3, and is discharged through the water outlet pipe 6.
[0019] Further, the upper portion of the first cylinder 1 is provided with a separating funnel 8, the outlet of the separating funnel 8 is connected with a rubber pipe, and the rubber pipe is connected with the cavity between the outer wall of the vertical pipe 3 and the inner wall of the second cylinder 2.
[0020] In the embodiment, whether the separating funnel 8 is used to separate the mixed solution of plasma exosome can be selected according to actual needs, and the separated liquid is introduced into the first cylinder 1.
[0021] Further, one side of the first cylinder 1 is provided with a support 9, the top of the support 9 is provided with a fixing clamp, and the fixing clamp is fixedly connected with the support 9, and the fixing clamp clamps the separating funnel 8.
[0022] In the embodiment, the support 9 and the fixing clamp are arranged, so that the separating funnel 8 is conveniently fixed.
[0023] Further, the top surface of the first cylinder 1 is provided with a cylinder cover, the cylinder cover is provided with a connecting pipe 7, the bottom of the connecting pipe 7 extends into the cavity between the outer wall of the vertical pipe 3 and the inner wall of the second cylinder 2, and the top of the connecting pipe 7 is connected with the rubber pipe of the outlet of the separating funnel 8.
[0024] In the embodiment, the cylinder cover is arranged to facilitate sealing.
[0025] Further, the bottom surface of the cylinder cover is sleeved on the second cylinder 2, and the cylinder cover is threadedly connected with the second cylinder 2.
[0026] In the embodiment, the cylinder cover is threadedly connected with the second cylinder 2, so that the vertical pipe 3 is conveniently opened, and the filter paper layer 5 is conveniently placed.
[0027] The working principle and use process of the utility model are as follows: after the utility model is installed, the above embodiment is used until all the working steps are completed.
[0028] The basic principle, main characteristics and advantages of the present application are shown and described above. It is obvious for those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or essential characteristics of the present application. Therefore, from any point of view, the embodiments should be considered as exemplary and non-limiting. The scope of the present application is defined by the appended claims rather than the above description, and therefore all changes falling within the meaning and scope of the equivalent elements of the claims should be included in the present application. Any reference signs in the claims should not be considered as limiting the claims involved.
[0029] Although the embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.
Claims
1. A high-efficiency plasma exosome filtration device, characterized in that: The invention comprises a first cylinder (1), wherein a second cylinder (2) is provided inside the first cylinder (1), a vertical tube (3) is provided inside the second cylinder (2), a cavity is formed between the outer wall of the vertical tube (3) and the inner wall of the second cylinder (2), the interior of the cavity is filled with silica gel, the two ends of the vertical tube (3) are through-set, and the bottom of the vertical tube (3) passes through the bottom surface of the second cylinder (2), a water outlet pipe (6) is provided on one side of the bottom of the first cylinder (1), a through hole (4) is provided through the side wall of the vertical tube (3), a filter paper layer (5) is provided inside the vertical tube (3), the filter paper layer (5) is in contact with the inner wall of the vertical tube (3) and seals the through hole (4).
2. A high-efficiency plasma exosome filtration device according to claim 1, characterized in that: A separating funnel (8) is provided above the first cylinder (1), and the outlet of the separating funnel (8) is connected to a rubber tube, which is in communication with the cavity between the outer wall of the vertical tube (3) and the inner wall of the second cylinder (2).
3. The high-efficiency plasma exosome filtration device according to claim 2, characterized in that: A bracket (9) is provided on one side of the first cylinder (1), and a fixing clamp is provided on the top of the bracket (9), and the fixing clamp clamps the separating funnel (8).
4. The high-efficiency plasma exosome filtration device according to claim 3, characterized in that: The top surface of the first cylinder (1) is provided with a cylinder cover, and the cylinder cover is penetrated by a connecting pipe (7). The bottom of the connecting pipe (7) extends into the cavity between the outer wall of the vertical pipe (3) and the inner wall of the second cylinder (2), and the top of the connecting pipe (7) is connected to the rubber tube at the outlet of the separating funnel (8).
5. The high-efficiency plasma exosome filtration device according to claim 4, characterized in that: The bottom surface of the cylinder cover is sleeved on the second cylinder body (2) and is threadedly connected.