Double-layer culture dish for anaerobic microorganisms
The anaerobic environment is constructed by a double-layer culture dish design and a sealing component, which solves the high cost and complex operation problems of anaerobic microbial culture in the existing technology, and achieves ease of use and efficient anaerobic maintenance.
Patent Information
- Application Number
- CN202422674685.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-04
- Publication Date
- 2025-10-17
- Estimated Expiration
- 2034-11-04
AI Technical Summary
Existing anaerobic microbial culture methods are costly and cumbersome to operate, which is not conducive to their widespread research and application.
The double-layer culture dish design is adopted, the culture space is separated from the deoxidizer by a partition, and the exhaust pipe and sealing components are used to build and maintain the anaerobic environment, which simplifies the operation and improves the sealing.
The ease of use and durability of anaerobic microbial culture are achieved, the sealing and anaerobic holding capabilities of the culture dish are improved, and the operational complexity and cost are reduced.
Smart Images
Figure CN223445531U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to experimental equipment technical field especially relates to an anaerobic microorganism double -layered culture dish. BACKGROUND
[0002] Microorganism culture dish is the vessel commonly used in laboratory, mainly used for the culture of microorganism or cell, and glass culture dish can be used for plant material, microorganism culture and animal cell adherent culture.
[0003] The sensitivity of anaerobic microorganism to oxygen makes its culture process need to strictly control oxygen content, and the traditional anaerobic culture method usually needs to be carried out in an oxygen-free environment, which needs to use complex equipment and technology to ensure the oxygen-free state of the culture environment. However, these methods are not only costly, but also cumbersome to operate, which is not conducive to the extensive research and application of anaerobic microorganism. UTILITY MODEL CONTENT
[0004] The utility model aims at solving the shortcomings that the anaerobic microorganism culture method in the prior art is not only costly, but also cumbersome to operate, which is not conducive to the extensive research and application of anaerobic microorganism, and provides an anaerobic microorganism double -layered culture dish.
[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme:
[0006] An anaerobic microorganism double -layered culture dish, including the dish bottom for placing anaerobic microorganism, the upper side of dish bottom is equipped with dish cover, the dish bottom and dish cover between are equipped with the baffle for placing deoxidizer;
[0007] Among them, the surface of the baffle is fixed with a fixed frame, a group of connecting components are arranged in the fixed frame, the connecting components are used for fixing the dish bottom and the dish cover on the upper and lower sides of the fixed frame respectively, and a sealing gap is formed between the dish bottom and the dish cover;
[0008] A plurality of communication grooves are uniformly arranged in the baffle and are communicated with the space in the dish bottom;
[0009] The exhaust pipe is communicated in the dish cover, the exhaust pipe is used for exhaust, a group of sealing components are arranged in the exhaust pipe, and the sealing components are used for maintaining the sealing property of the sealing gap after exhaust.
[0010] In a possible design, the connecting components include two internal threads arranged in the fixed frame, the surfaces of the dish bottom and the dish cover are provided with external threads, and the dish cover and the dish bottom are threadedly connected to the upper and lower sides of the fixed frame respectively.
[0011] In a possible design, the surfaces of the fixed frame and the dish cover are fixed with anti-skid rings.
[0012] In a possible design, the end of the dish bottom and the dish cover close to each other is fixed with a sealing gasket, and the two sealing gaskets are in extrusion contact with the upper and lower ends of the partition plate respectively.
[0013] In a possible design, the top end of the partition plate is uniformly fixed with a plurality of limiting communication barrels, the plurality of limiting communication barrels are in communication with the plurality of communication grooves respectively, and the plurality of limiting communication barrels are used for limiting the deoxidizing agent.
[0014] In a possible design, the sealing assembly comprises a gap plate fixed in the exhaust pipe, a connecting rod sliding in the gap plate, a sealing plate fixed at the side end of the connecting rod, a sealing ring fixed on the inner wall of the exhaust pipe and matched with the sealing plate, a sealing spring fixed between the gap plate and the sealing plate, and the sealing spring is sleeved on the surface of the connecting rod.
[0015] In a possible design, the dish bottom, the dish cover and the partition plate are made of transparent glass.
[0016] In the application, the anaerobic microorganism to be cultured is placed in the dish bottom for storage, the fixed frame is screwed and fixed to the surface of the dish bottom, the deoxidizing agent is placed on the partition plate in the fixed frame, the dish cover is fixed in the fixed frame by screwing the dish cover, the exhaust pipe is connected with the micro vacuum pump in the external environment, and the micro vacuum pump is started to extract the vacuum in the dish bottom. After the vacuum pump is closed, the elastic sealing spring pushes the sealing plate to adhere to the sealing ring, the sealing ring and the sealing plate cooperate to seal the space in the dish bottom, the space on the upper side of the partition plate is communicated with the space in the dish bottom through the plurality of limiting communication barrels, the plurality of communication grooves and the plurality of limiting communication barrels, and the deoxidizing agent can perform the deoxidation reaction on the space in the dish bottom to remove the oxygen in the dish bottom.
[0017] Beneficial effects: in the utility model, the anaerobic microorganism double-layer culture dish adopts a double-layer structure design, the culture space is separated from the deoxidizing agent by the partition plate, the effective action of the deoxidizing agent is ensured, and direct interference of the deoxidizing agent on the growth of microorganisms is avoided.
[0018] In the utility model, the anaerobic microorganism double-layer culture dish realizes easy fixing and dismounting of the dish bottom and the dish cover, and ensures the sealing performance of the culture dish. This design not only improves the usability of the culture dish, but also enhances the durability and reliability of the culture dish.
[0019] In the utility model, the deoxidizing agent is used to remove the oxygen in the culture dish, and the sealing maintenance after exhaust is realized through the exhaust pipe and the sealing assembly. This design not only simplifies the construction process of the anaerobic environment, but also improves the sealing performance and oxygen-free maintenance capacity of the culture dish. BRIEF DESCRIPTION OF DRAWINGS
[0020] Figure 1An explosion schematic view of the anaerobic microorganism double-layer culture dish is provided in the utility model.
[0021] Figure 2 A front view of the anaerobic microorganism double-layer culture dish is provided in the utility model.
[0022] Figure 3 A sectional view of the anaerobic microorganism double-layer culture dish is provided in the utility model.
[0023] Figure 4 A partial view of the anaerobic microorganism double-layer culture dish is provided in the utility model.
[0024] In the figure: 1, dish bottom; 2, dish cover; 3, partition; 4, fixed frame; 5, anti-skid ring; 6, communication groove; 7, limiting communication cylinder; 8, exhaust pipe; 9, sealing pad; 10, gap plate; 11, sealing ring; 12, sealing plate; 13, sealing spring; 14, connecting rod. DETAILED DESCRIPTION
[0025] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments.
[0026] Embodiment 1: refer to Figures 1-4 An anaerobic microorganism double-layer culture dish is applied in the technical field of experimental equipment, which comprises a dish bottom 1, a dish cover 2, a partition 3, a fixed frame 4, an anti-skid ring 5, a communication groove 6, a limiting communication cylinder 7, an exhaust pipe 8, a sealing pad 9, a gap plate 10, a sealing ring 11, a sealing plate 12, a sealing spring 13 and a connecting rod 14 and the like.
[0027] The dish bottom 1 is used for placing culture medium and microbial samples of anaerobic microorganisms. The upper side of the dish bottom 1 is provided with the dish cover 2, and the dish cover 2 and the dish bottom 1 are fixedly connected through the fixed frame 4 and a connecting assembly. The fixed frame 4 is fixed to the surface of the partition 3, and the connecting assembly comprises two internal threads arranged in the fixed frame 4 and external threads arranged on the surfaces of the dish bottom 1 and the dish cover 2 respectively. Through the threaded connection mode, the dish cover 2 and the dish bottom 1 are respectively fixed to the upper and lower sides of the fixed frame 4, and a sealing gap is formed between the two, so as to ensure the construction of the anaerobic environment.
[0028] In order to facilitate the user to rotate and fix the dish bottom 1 and the dish cover 2, the surfaces of the fixed frame 4 and the dish cover 2 are both fixed with the anti-skid ring 5. These anti-skid rings 5 provide additional gripping force and rotation stability, so that the user can more easily complete the fixing operation of the dish bottom 1 and the dish cover 2.
[0029] A plurality of communication grooves 6 are evenly formed in the partition plate 3, and the communication grooves 6 are communicated with the space in the dish bottom 1 and used for placing the deoxidizing agent. In order to limit the deoxidizing agent and prevent it from moving or leaking in the partition plate 3, a plurality of limiting communication tubes 7 are evenly fixed at the top end of the partition plate 3, and the limiting communication tubes 7 are communicated with the plurality of communication grooves 6 respectively.
[0030] An exhaust pipe 8 is further fixed and communicated in the partition plate 3, and the exhaust pipe 8 is used for exhaust operation in the initial stage of culture to remove the residual oxygen in the culture dish. In order to ensure the sealing performance after exhaust, a sealing assembly is arranged in the exhaust pipe 8. The sealing assembly comprises a gap plate 10 fixed in the exhaust pipe 8, and a connecting rod 14 slidingly arranged in the gap plate 10. The side end of the connecting rod 14 is fixed with a sealing plate 12, and the inner wall of the exhaust pipe 8 is fixed with a sealing ring 11 matched with the sealing plate 12. In addition, a sealing spring 13 is further fixed between the gap plate 10 and the sealing plate 12, and the sealing spring 13 is sleeved on the surface of the connecting rod 14. When the exhaust pipe 8 performs exhaust, the sealing plate 12 is pushed away by the gas pressure; and when the exhaust is completed, the sealing spring 13 pushes the sealing plate 12 to be in close contact with the sealing ring 11 again, so as to maintain the sealing performance of the sealing gap.
[0031] Embodiment 2: Reference Figures 1-4 On the basis of Embodiment 1, the improvement is that: in order to ensure the sealing effect between the dish bottom 1 and the dish cover 2, the end portions of the dish bottom 1 and the dish cover 2 close to each other are fixed with sealing gaskets 9. The two sealing gaskets 9 are respectively in extrusion contact with the upper and lower ends of the partition plate 3, and the sealing performance of the culture dish is further enhanced.
[0032] In addition, in order to facilitate the observation of the growth of microorganisms in the culture dish, the dish bottom 1, the dish cover 2 and the partition plate 3 are all made of transparent glass. This design enables the user to directly observe the growth state of the microorganisms and the change of the culture medium without opening the culture dish.
[0033] The above merely describes the preferred embodiments of the present application, but the protection scope of the present application is not limited to this. Any skilled person in the art can make equivalent replacement or change according to the technical scheme and the inventive concept of the present application within the technical range disclosed by the present application, and all of them should be covered in the protection scope of the present application.
Claims
1. A double-layer culture dish for anaerobic microorganisms, characterized in that: include: A dish bottom (1) for placing anaerobic microorganisms, a dish cover (2) is provided on the upper side of the dish bottom (1), and a partition (3) for placing a deoxidizer is provided between the dish bottom (1) and the dish cover (2); A fixing frame (4) is fixed on the surface of the partition (3), and a group of connecting components are provided in the fixing frame (4). The connecting components are used to fix the dish bottom (1) and the dish cover (2) to the upper and lower sides of the fixing frame (4), respectively, and a sealing gap is formed between the dish bottom (1) and the dish cover (2); The partition (3) is evenly provided with a plurality of communication grooves (6) that are connected to the space in the bottom of the dish (1); An exhaust pipe (8) is fixedly connected to the dish cover (2), and the exhaust pipe (8) is used for exhausting gas. A set of sealing components is provided in the exhaust pipe (8), and the sealing components are used to maintain the sealing performance of the sealing gap after exhausting gas.
2. The anaerobic microbial double-layer culture dish according to claim 1, characterized in that: The connection assembly comprises two internal threads provided in the fixing frame (4), and the surfaces of the dish bottom (1) and the dish cover (2) are both provided with external threads, and the dish cover (2) and the dish bottom (1) are respectively threadedly connected to the upper and lower sides of the fixing frame (4).
3. The double-layer culture dish for anaerobic microorganisms according to claim 2, characterized in that: Anti-slip twist rings (5) are fixed on the surfaces of the fixing frame (4) and the dish cover (2).
4. The double-layer culture dish for anaerobic microorganisms according to claim 2, characterized in that: Sealing pads (9) are fixed to the adjacent ends of the dish bottom (1) and the dish cover (2), and the two sealing pads (9) are respectively in compression contact with the upper and lower ends of the partition (3).
5. The double-layer culture dish for anaerobic microorganisms according to claim 2, characterized in that: A plurality of position-limiting connecting tubes (7) are evenly fixed on the top of the partition (3), and the plurality of position-limiting connecting tubes (7) are respectively connected to the plurality of connecting grooves (6). The plurality of position-limiting connecting tubes (7) are used to limit the deoxidizer.
6. The double-layer culture dish for anaerobic microorganisms according to any one of claims 1 to 3, characterized in that: The sealing assembly includes a gap plate (10) fixed in the exhaust pipe (8), a connecting rod (14) sliding in the gap plate (10), a sealing plate (12) fixed to the side end of the connecting rod (14), a sealing ring (11) used in conjunction with the sealing plate (12) fixed to the inner wall of the exhaust pipe (8), a sealing spring (13) fixed between the gap plate (10) and the sealing plate (12), and the sealing spring (13) sleeved on the surface of the connecting rod (14).
7. The double-layer culture dish for anaerobic microorganisms according to claim 1, characterized in that: The dish bottom (1), dish cover (2) and partition (3) are all made of transparent glass.