High-pressure homogenizer for purifying protein

Abstract

The utility model relates to the technical field of high-pressure homogenizers, in particular to a high-pressure homogenizer for purifying protein, which comprises a feeding box mounted on a machine body, and a bubble removing mechanism for removing bubbles in protein turbid liquid is arranged in the feeding box. A high-pressure homogenizer for performing high-pressure homogenization on the protein suspension is arranged on the machine body. According to the utility model, the rotating motor drives the rotating shaft to rotate, so that the impeller at the bottom of the rotating shaft is driven to rotate to generate centrifugal force, and a bubble liquid film is broken by utilizing the centrifugal force generated by rotation, so that gas is discharged, and bubbles in protein turbid liquid are eliminated; the high-speed jet fluid generates pressure drop, so that bubbles and protein turbid liquid are mixed in the Venturi jet device and then discharged through a diffusion section in the Venturi jet device, and the effect of removing the bubbles is achieved through the pressure drop and suction force generated by the high-speed fluid.

Description

TECHNICAL FIELD

[0001] The utility model relates to high pressure homogenizer technical field especially relates to a high pressure homogenizer for purifying protein. BACKGROUND

[0002] High pressure homogenizer is mainly used in the cell breakage of biological technology field, the research and development of special reagent of medical field, material homogenization of cosmetic field, substance emulsification of food industry and so on, and in the process of purifying protein, high pressure homogenizer plays an important role, and it is helpful to improve the homogeneity and stability of protein and is suitable for protein purification process of different scales and needs.

[0003] But the existing high pressure homogenizer carries out high pressure homogenization to the purified protein, and the protein suspension contains a certain amount of bubbles, which leads to the empty condition in the pipeline during the homogenization process, and further influences the service life of the homogenization valve, and the protein suspension contains larger particles or certain impurities, which will affect the homogenization valve. UTILITY MODEL CONTENT

[0004] In view of the defects of the prior art, the utility model provides a high pressure homogenizer for purifying protein, which solves the technical problems that the protein suspension contains bubbles in the prior art, leads to the empty condition in the pipeline, and influences the service life of the homogenization valve, and achieves the purpose of removing the bubbles in the suspension.

[0005] To solve the above technical problems, the utility model provides the following technical scheme: a high pressure homogenizer for purifying protein, which comprises a feed tank installed on a machine body, a bubble removal mechanism for removing bubbles in the protein suspension is arranged in the feed tank, and a high pressure homogenization mechanism for high pressure homogenization of the protein suspension is arranged on the machine body.

[0006] The bubble removal mechanism comprises a partition plate installed in the feed tank, a rotating shaft is rotatably connected in a rotating hole formed in the top center of the feed tank, an impeller is installed at the bottom of the rotating shaft, a rotating motor for driving the rotating shaft to rotate is installed on the feed tank, a secondary elimination assembly for secondary elimination of bubbles in the protein suspension is installed on the side of the feed tank, an elimination feeding assembly for tertiary elimination of bubbles in the protein suspension is arranged at the bottom of the feed tank, and a feed filtering assembly for filtering and intercepting large particles or impurities in the protein suspension is arranged on the feed tank.

[0007] Preferably, the secondary elimination assembly comprises a communication pipe installed on the side of the feed tank, the communication pipe is in U-shaped structure, a suction pump is installed on the communication pipe, a venturi jet is installed at the bottom of the communication pipe, and the venturi jet extends into the interior of the feed tank.

[0008] Preferably, the eliminating feeding assembly comprises a feeding pipe installed at the bottom of the feeding tank, an ultrafiltration membrane is installed in the feeding pipe, and a one-way valve is installed on the feeding pipe.

[0009] Preferably, the feeding filtering assembly comprises a feeding pipe installed at the top of the feeding tank, a filling bucket is sleeved on the feeding pipe, and a filtering dense net is installed in the filling bucket.

[0010] Preferably, the high-pressure homogenizing mechanism comprises a piston power tank installed on the machine body, a pipeline is installed in the piston power tank, the feeding pipe extends into the pipeline, a homogenizing valve is installed on the pipeline, an electric telescopic rod is installed at the right end of the pipeline, the free end of the electric telescopic rod extends into the pipeline, and a striking head is installed on the free end, and a discharging pipe is installed at the bottom of the pipeline.

[0011] Preferably, a controller is installed on the machine body, and the bubble removing mechanism and the high-pressure homogenizing mechanism are electrically connected to the controller.

[0012] By means of the above technical scheme, the high-pressure homogenizing machine for purifying protein has at least the following beneficial effects:

[0013] 1. The rotating shaft is driven to rotate by the rotating motor, so that the impeller at the bottom of the rotating shaft is driven to rotate to generate centrifugal force, the centrifugal force generated by rotation is utilized to break the bubble liquid film, and the gas is discharged, and the bubbles in the protein suspension liquid are eliminated.

[0014] 2. When the protein suspension liquid enters the injection cavity from the inlet of the Venturi jet, high-speed injection fluid is formed, the pressure drop of the high-speed fluid is generated, the bubbles and the protein suspension liquid are mixed in the Venturi jet, and then are discharged through the diffusion section in the Venturi jet, so that the effect of removing the bubbles is realized through the pressure drop and suction force generated by the high-speed fluid. BRIEF DESCRIPTION OF DRAWINGS

[0015] The accompanying drawings, which are included to provide a further understanding of the application and constitute a part of this application, illustrate certain illustrative embodiments of the application and together with the description serve to explain the application.

[0016] In the drawings:

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 It is a schematic diagram of the independent structure of the feeding tank of the utility model;

[0019] Figure 3 It is a schematic diagram of the internal structure of the feeding tank of the utility model;

[0020] Figure 4 It is a high-pressure homogenizing mechanism sectional view internal structure schematic view of the utility model;

[0021] Figure 5 It is a high-pressure homogenous mechanism local amplification structure schematic view of the utility model.

[0022] In the drawing: 1, machine body;2, feed tank;3, bubble removal mechanism;301, partition plate;302, rotating shaft;303, impeller;304, rotating motor;305, secondary elimination assembly;3051, communication pipe;3052, suction pump;3053, venturi jet;306, eliminate feeding assembly;3061, feeding pipe;3062, ultrafiltration membrane;3063, check valve;307, feed filter assembly;3071, injection pipe;3072, filling hopper;3073, filter dense net;4, high-pressure homogenizing mechanism;401, piston power box;402, pipeline;403, homogenizing valve;404, electric telescopic rod;405, impact head;406, discharge pipe;5, controller. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0024] Embodiment 1

[0025] Based on the problem that the existing protein suspension liquid contains bubbles, resulting in empty running in the pipeline, affecting the service life of the homogenizing valve in the prior art, the embodiment provides a high-pressure homogenizer for purifying protein, please refer to Figures 1-5 , the embodiment provides a high-pressure homogenizer for purifying protein, which can remove bubbles in the suspension liquid. The high-pressure homogenizer for purifying protein comprises a feed tank 2 installed on a machine body 1, a bubble removal mechanism 3 for eliminating bubbles in the protein suspension liquid is arranged in the feed tank 2, a high-pressure homogenizing mechanism 4 for homogenizing the protein suspension liquid is arranged on the machine body 1, the bubbles in the protein suspension liquid are eliminated by the bubble removal mechanism 3, avoiding the empty running of the pipeline 402 in the homogenizing process, affecting the service life of the homogenizing valve 403, and the gap between the homogenizing valve 403 and the impact head 405 is adjusted by the high-pressure homogenizing mechanism 4 to adapt to different use conditions.

[0026] Since the protein suspension liquid in the prior art contains bubbles, which causes the pipeline 402 to be empty, affecting the service life of the homogenizing valve 403, therefore, the device is provided with a bubble removing mechanism 3, the bubble removing mechanism 3 comprises a partition plate 301 installed in the inside of the feeding tank 2, a rotating shaft 302 is rotatably connected in the rotating hole opened in the top center of the feeding tank 2, and the bottom of the rotating shaft 302 is installed with an impeller 303, the feeding tank 2 is installed with a rotating motor 304 for driving the rotating shaft 302 to rotate, the side of the feeding tank 2 is installed with a secondary elimination assembly 305 for eliminating the bubbles in the protein suspension liquid for the second time, the bottom of the feeding tank 2 is provided with an elimination feeding assembly 306 for eliminating the bubbles in the protein suspension liquid for the third time, and the feeding tank 2 is provided with a feeding filtering assembly 307 for filtering and intercepting the large particles or impurities in the protein suspension liquid, the rotating motor 304 drives the rotating shaft 302 to rotate, thereby driving the impeller 303 at the bottom of the rotating shaft 302 to rotate to generate centrifugal force, the centrifugal force generated by the rotation breaks the bubble liquid film, thereby discharging the gas and eliminating the bubbles in the protein suspension liquid.

[0027] In order to avoid that the micro bubbles in the protein suspension liquid are not eliminated, therefore, the device is also provided with the elimination feeding assembly 306, the elimination feeding assembly 306 comprises a feeding pipe 3061 installed at the bottom of the feeding tank 2, an ultrafiltration membrane 3062 is installed in the feeding pipe 3061, and a one-way valve 3063 is installed on the feeding pipe 3061, the protein suspension liquid is transported to the inside of the high-pressure homogenizing mechanism 4 through the feeding pipe 3061, in this process, the ultrafiltration membrane 3062 in the feeding pipe 3061 intercepts and defoams the remaining micro bubbles in the protein suspension liquid, further removing the bubbles.

[0028] In order to avoid that the protein suspension liquid is mixed with large particles or impurities, causing the mechanical parts in the high-pressure homogenizing mechanism 4 to be damaged, therefore, the device is also provided with the feeding filtering assembly 307, the feeding filtering assembly 307 comprises a feeding pipe 3071 installed at the top of the feeding tank 2, a filling bucket 3072 is sleeved on the feeding pipe 3071, a filtering dense net 3073 is installed in the filling bucket 3072, the filling bucket 3072 is placed in the feeding pipe 3071, then the protein suspension liquid is injected into the filling bucket 3072 and flows into the feeding tank 2, in this process, the protein suspension liquid flows through the filtering dense net 3073 in the filling bucket 3072, thereby intercepting and filtering the large particles or impurities in the protein suspension liquid.

[0029] In order to better adapt to different specifications of protein suspension high pressure homogenization processing, the device is also provided with a high pressure homogenization mechanism 4, the high pressure homogenization mechanism 4 includes a piston power box 401 mounted on the machine body 1, a pipeline 402 is mounted in the piston power box 401, and the feeding pipe 3061 extends into the pipeline 402, the homogenization valve 403 is mounted on the pipeline 402, the electric telescopic rod 404 is mounted at the right end of the pipeline 402, the free end of the electric telescopic rod 404 extends into the pipeline 402, and the impact head 405 is mounted, the discharge pipe 406 is mounted at the bottom of the pipeline 402, the protein suspension in the feeding tank 2 is extruded and conveyed to the homogenization valve 403 on the pipeline 402 through the piston power box 401, and the electric telescopic rod 404 can be started to pull the impact head 405 left and right according to different specifications of protein suspension, so as to adjust the gap between the impact head 405 and the homogenization valve 403, so as to adapt to different protein suspensions.

[0030] The controller 5 is mounted on the machine body 1, and the bubble removal mechanism 3 and the high pressure homogenization mechanism 4 are electrically connected to the controller 5, and the normal operation of the device is controlled through the controller 5.

[0031] Example 2

[0032] Based on example 1, as shown in Figures 1-5 Because part of the micro-bubbles is not eliminated in the centrifugal rotation process, and a certain amount of micro-bubbles is also generated in the centrifugal rotation process, the device is also provided with a secondary elimination assembly 305, the secondary elimination assembly 305 includes a communication pipe 3051 mounted on the side of the feeding tank 2, and the communication pipe 3051 is in U-shaped structure, the suction pump 3052 is mounted on the communication pipe 3051, and the venturi jet 3053 is mounted at the bottom of the communication pipe 3051, and the venturi jet 3053 extends into the inside of the feeding tank 2, the suction pump 3052 is started to pump the protein suspension in which the first bubbles are eliminated by centrifugal to the venturi jet 3053 at the bottom of the communication pipe 3051 through the communication pipe 3051, when the protein suspension enters the jet chamber from the inlet of the venturi jet 3053, high-speed jet fluid is formed, the high-speed fluid generates pressure drop, so that the bubbles and the protein suspension are mixed in the venturi jet 3053, and then discharged through the diffusion section in the venturi jet 3053, so as to realize the effect of removing bubbles through the pressure drop and suction force generated by the high-speed fluid.

[0033] It should be noted that in this paper, the term "including", "containing" or any other variant thereof is intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed, or includes elements inherent to such process, method, article or equipment.

[0034] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A high pressure homogeniser for purifying proteins, comprising a feed tank (2) mounted on a machine body (1), characterised in that: The feed tank (2) is provided with a bubble removal mechanism (3) for removing bubbles in the protein suspension, and the machine body (1) is provided with a high-pressure homogenization mechanism (4) for high-pressure homogenization of the protein suspension. The bubble removal mechanism (3) comprises a partition plate (301) installed in the feed tank (2), a rotating shaft (302) rotatably connected in a rotating hole opened in the center of the top of the feed tank (2), and an impeller (303) installed at the bottom of the rotating shaft (302), a rotating motor (304) installed on the feed tank (2) for driving the rotating shaft (302) to rotate, a secondary elimination assembly (305) installed on the side of the feed tank (2) for secondary elimination of bubbles in the protein suspension, an elimination feeding assembly (306) provided at the bottom of the feed tank (2) for tertiary elimination of bubbles in the protein suspension, and a feed filtering assembly (307) provided on the feed tank (2) for filtering and intercepting large particles or impurities in the protein suspension.

2. The high-pressure homogenizer for purifying a protein according to claim 1, characterized by: The secondary elimination assembly (305) comprises a communication pipe (3051) installed on the side of the feed tank (2), wherein the communication pipe (3051) is in a U-shaped structure, a suction pump (3052) is installed on the communication pipe (3051), and a Venturi jet (3053) is installed at the bottom of the communication pipe (3051) and extends into the interior of the feed tank (2).

3. The high-pressure homogenizer for purifying a protein according to claim 1, characterized by: The elimination feeding assembly (306) comprises a feeding pipe (3061) installed at the bottom of the feed tank (2), wherein an ultrafiltration membrane (3062) is installed in the feeding pipe (3061), and a one-way valve (3063) is installed on the feeding pipe (3061).

4. The high-pressure homogenizer for purifying a protein according to claim 1, characterized by: The feed filtering assembly (307) comprises a feeding pipe (3071) installed on the top of the feed tank (2), wherein a filling bucket (3072) is sleeved on the feeding pipe (3071), and a filtering dense net (3073) is installed in the filling bucket (3072).

5. The high-pressure homogenizer for purifying a protein according to claim 1, characterized by: The high-pressure homogenization mechanism (4) comprises a piston power tank (401) installed on the machine body (1), a pipeline (402) installed in the piston power tank (401), wherein the feeding pipe (3061) extends into the pipeline (402), a homogenization valve (403) installed on the pipeline (402), an electric telescopic rod (404) installed at the right end of the pipeline (402), wherein the free end of the electric telescopic rod (404) extends into the pipeline (402) and is provided with an impact head (405), and a discharge pipe (406) installed at the bottom of the pipeline (402).

6. The high-pressure homogenizer for purifying a protein according to claim 1, characterized by: A controller (5) is installed on the machine body (1), and the bubble removal mechanism (3) and the high-pressure homogenization mechanism (4) are electrically connected to the controller (5).

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