Biological enzyme purification device

Abstract

The utility model provides a biological enzyme purification device, and belongs to the technical field of biological enzyme preparation equipment. Comprising a mounting rack, a first barrel is arranged on the mounting rack, a rough filtration mechanism is arranged in the first barrel, a first liquid inlet pipe is arranged at the top of the first barrel, an air inlet pipe is connected to the outer wall of the first barrel, a mounting box is arranged on the mounting rack, the first barrel communicates with the mounting box, a blow-off pipe is connected to the bottom of the mounting box, and an exhaust pipe is connected to the top of the mounting box; a fine filtration mechanism and a second liquid outlet pipe are connected to the side wall of one side of the mounting box, and a valve body is arranged in the exhaust pipe. In the process that a culture solution is fed into the mounting box from the first barrel, the culture solution gradually fills the mounting box, gas is exhausted from the exhaust pipe, the valve body closes the exhaust pipe, the culture solution is filtered by the filter layer and then exhausted, a purified biological enzyme solution is obtained, and when the culture solution is lowered to the position below the top of the filter layer, the valve is opened again to exhaust gas in time. Bubbles are prevented from being generated in the bio-enzyme solution, and the purification effect of the bio-enzyme solution is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological enzyme preparation equipment technical field especially relates to a biological enzyme purification device. BACKGROUND

[0002] In the production of protease, purification and separation are needed, which is briefly described as follows: in the extraction process of protease, biological tissues are first broken, then the clarified liquid is taken and a reagent is added, and then the liquid is poured into an enzyme extraction device, impurities are filtered out through the device, and a liquid with high protease content is obtained.

[0003] The patent with publication number CN212925016U discloses a protease purification device, which comprises a support frame, a shell fixed on the support frame, and a first cylinder fixed on the shell. The interior of the first cylinder is provided with a centrifugal filtration mechanism for centrifugal filtration of a solution containing protease. The shell is provided with a centrifugal power mechanism connected to the centrifugal filtration mechanism. The shell is also provided with a fine filtration mechanism. The shell is also provided with a liquid power mechanism, and the liquid power mechanism sends the liquid centrifugally filtered in the first cylinder to the fine filtration mechanism. The liquid power mechanism is a pump body, the liquid inlet end of which is connected to the first cylinder, and the liquid outlet end of which is connected to the fine filtration mechanism.

[0004] However, in actual use, the experimenters cannot accurately observe the content of the solution preliminarily filtered in the first cylinder. After the solution in the first cylinder is completely pumped into the fine filtration mechanism by the pump body, the pump body will be idle, which can easily cause wear of the pump body and foaming of the biologically purified enzyme solution, reducing the service life of the pump body and affecting subsequent experiments. Utility model content

[0005] In view of the above problems, the utility model provides a biological enzyme purification device.

[0006] In order to achieve the above-mentioned purposes, the utility model adopts the following technical solutions:

[0007] The utility model provides a biological enzyme purification device, which comprises a mounting rack, a first cylinder arranged on the mounting rack, a coarse filtration mechanism arranged in the first cylinder, a first liquid inlet pipe arranged on the top of the first cylinder and facing the coarse filtration mechanism, a first valve connected to the first liquid inlet pipe, a mounting box arranged below the first cylinder on the mounting rack, a first liquid outlet pipe connected to the bottom of the first cylinder, the other end of the first liquid outlet pipe connected to the mounting box, a blowdown pipe connected to the bottom of the mounting box, an exhaust pipe connected to the top of the mounting box, an air inlet pipe connected to the outer wall of the first cylinder, the air inlet pipe connected to an air source, a second liquid outlet pipe connected to one side wall of the mounting box, a fine filtration mechanism arranged between the mounting box and the second liquid outlet pipe, and a valve body arranged in the exhaust pipe for emptying the air in the mounting box and limiting the solution from entering the exhaust pipe.

[0008] Further, the coarse filter mechanism comprises a second cylinder body rotatably arranged in the first cylinder body, the top of the second cylinder body is open, the side wall of the second cylinder body is hollow, the first driving motor is arranged on the mounting frame, the output shaft of the first driving motor extends into the first cylinder body and is fixedly connected with the bottom wall of the second cylinder body in a coaxial manner, and the filter sleeve is fixedly arranged on the inner wall of the second cylinder body in a coaxial manner.

[0009] Further, the fine filter mechanism comprises a mounting frame and a filter layer, the mounting frame is arranged on the side wall of the mounting box, the mounting port is arranged on the mounting frame and communicates the mounting box and the second liquid outlet pipe, and the filter layer is arranged in the mounting port.

[0010] Further, the bottom of the blow-off pipe is detachably connected with the sewage collecting tank.

[0011] Further, the valve body comprises a floating ball and a connecting frame, the exhaust port is arranged in the mounting box, the exhaust pipe communicates with the exhaust port, the connecting frame is arranged in the mounting box and located below the exhaust port, the floating ball is vertically movably arranged in the connecting frame, and the floating ball can block the exhaust port upwardly.

[0012] Further, the exhaust pipe is connected with the anti-spray assembly, the anti-spray assembly comprises a first anti-spray cover and a second anti-spray cover which are sequentially arranged outside the outlet of the exhaust pipe, a plurality of first outlets and a plurality of second outlets are evenly arranged on the side walls of the first anti-spray cover and the second anti-spray cover in a circumferential direction of the first anti-spray cover and the second anti-spray cover respectively, and the first outlets and the second outlets are staggered with each other.

[0013] Further, the inner top wall of the mounting box is arranged as a guide groove in an inverted conical shape, and the exhaust port is connected to the top end of the guide groove.

[0014] The beneficial effects of the present application are as follows: when the biological enzyme is purified, the culture solution is injected into the second cylinder body in the first cylinder body through the first liquid inlet pipe, the first liquid inlet pipe is closed, the second cylinder body is rotated, the culture solution in the second cylinder body is initially filtered from the filter sleeve on the side wall of the second cylinder body, after the initial filtration, the first cylinder body is inflated by the gas source through the air inlet pipe, the culture solution in the first cylinder body is sent into the mounting box through the first liquid outlet pipe, when the air in the mounting box is exhausted and the culture solution fills the mounting box, the valve body closes the exhaust pipe and forces the culture solution to be filtered through the filter layer and then discharged, and the purified biological enzyme solution is obtained and discharged from the second liquid outlet pipe, when the culture solution in the mounting box is lowered below the top of the filter layer, the valve opens the exhaust pipe, air is prevented from being discharged from the second liquid outlet pipe to cause bubbles in the biological enzyme solution, and the purification effect of the biological enzyme solution is improved. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a whole structure schematic view of the biological enzyme purification device of the embodiment of the present application.

[0016] Figure 2 It is a whole structure schematic view of the biological enzyme purification device of the embodiment of the present application. Figure 1 It is a local enlarged schematic view of part A.

[0017] 1, mounting rack; 11, first cylinder; 111, first liquid outlet pipe; 112, air inlet pipe; 12, first liquid inlet pipe; 121, first valve; 21, second cylinder; 22, first driving motor; 23, filter sleeve; 3, mounting box; 31, air outlet pipe; 32, second liquid outlet pipe; 33, guide groove; 5, sewage pipe; 51, sewage collecting tank; 61, mounting frame; 62, filter layer; 71, floating ball; 72, connecting frame; 73, air outlet; 81, first blowout prevention cover; 82, second blowout prevention cover. DETAILED DESCRIPTION

[0018] In order to better understand the above technical solutions, the above technical solutions will be described in detail below in combination with the drawings of the specification and specific embodiments.

[0019] The embodiment of the present application discloses a biological enzyme purification device, referring to Figure 1 and Figure 2 , comprising a mounting rack 1, the mounting rack 1 is provided with a first cylinder 11, the first cylinder 11 is hollow inside and is provided with a coarse filter mechanism, the top of the first cylinder 11 is connected with a first liquid inlet pipe 12, the first liquid inlet pipe 12 is connected with a valve, the end of the first liquid inlet pipe 12 extending into the first cylinder 11 is opposite to the coarse filter mechanism, the biological enzyme culture solution enters the first cylinder 11 from the first liquid inlet pipe 12 and is released into the coarse filter mechanism. The mounting rack 1 is provided with a mounting box 3 below the first cylinder 11, the bottom of the first cylinder 11 is connected with a first liquid outlet pipe 111, the other end of the first liquid outlet pipe 111 is connected with the mounting box 3. The outer wall of the first cylinder 11 is connected with an air inlet pipe 112, the air inlet pipe 112 is connected with an air source, the air source can be clean air, the bottom of the mounting box 3 is connected with a sewage pipe 5, the top of the mounting box 3 is connected with an air outlet pipe 31, the side wall of the mounting box 3 is connected with a second liquid outlet pipe 32, a fine filter mechanism is arranged between the mounting box 3 and the second liquid outlet pipe 32. The top wall of the mounting box 3 is connected with the air outlet pipe 31, a valve body is arranged in the air outlet pipe 31 for exhausting air in the mounting box 3 and limiting the solution from entering the air outlet pipe 31.

[0020] When the device is running, the biological enzyme culture solution is coarsely filtered by the coarse filter mechanism, the culture solution enters the first cylinder 11, the air source is used to inject air into the first cylinder 11, so that the culture solution in the first cylinder 11 enters the mounting box 3 from the first liquid outlet pipe 111 at the bottom of the first cylinder 11. In the mounting box 3, the original air is exhausted from the air outlet pipe 31 through the valve body, until the mounting box 3 is filled with the culture solution, the valve body closes the air outlet pipe 31, and the air injection into the first cylinder 11 continues, so that the culture solution is squeezed to pass through the fine filter mechanism, the filtered and purified biological enzyme solution is obtained, and the filtered impurities are precipitated in the mounting box 3 and stored in the sewage pipe 5.

[0021] In the embodiment of the present application, the coarse filter mechanism comprises a second cylinder 21 rotatably arranged in the first cylinder 11, the top of the second cylinder 21 is open, the side wall of the second cylinder 21 is hollow, and a filter sleeve 23 is coaxially fixedly installed on the inner wall of the second cylinder 21. A first driving motor 22 is fixedly installed inside the mounting rack 1, the output shaft of the first driving motor 22 extends into the first cylinder 11 and is coaxially fixedly connected to the bottom wall of the second cylinder 21, and one end of the first liquid inlet pipe 12 extending into the first cylinder 11 is located directly above the opening of the second cylinder 21. The first driving motor 22 is operated to drive the second cylinder 21 to rotate, so that the culture solution in the second cylinder 21 is subjected to centrifugal force and adheres to the inner wall of the filter sleeve, and is discharged into the first cylinder 11 after being filtered by the filter sleeve.

[0022] The fine filter mechanism comprises a mounting frame 61 and a filter layer 62. The mounting frame 61 is arranged on the side wall of the mounting box 3, and a mounting opening is formed through the mounting frame 61 to communicate the mounting box 3 and the second liquid outlet pipe 32. The filter layer 62 is arranged in the mounting opening, and the pore size of the filter layer 62 is smaller than that of the filter sleeve 23.

[0023] In the embodiment of the present application, the end of the blow-off pipe 5 away from the mounting box 3 is detachably connected to a dirt collecting tank 51, which is used to collect the impurities filtered in the mounting box 3. The dirt collecting tank 51 and the blow-off pipe 5 can be connected by a threaded connection. In other embodiments, a second valve can be installed on the blow-off pipe 5 to control the opening and closing of the blow-off pipe 5.

[0024] In the embodiment of the present application, the valve body comprises a floating ball 71 and a connecting frame 72. An exhaust opening 73 is formed in the mounting box 3, and the exhaust opening 73 should be higher than the upper edge of the filter layer 62 in the fine filter mechanism. An exhaust pipe 31 communicates with the exhaust opening 73. The connecting frame 72 is a hollow frame fixedly installed on the inner top wall of the mounting box 3 and located directly below the exhaust opening 73. The floating ball 71 is vertically movably arranged in the connecting frame 72. The floating ball 71 can be a hollow plastic ball with a rubber outer layer on the surface of the plastic ball. The diameter of the floating ball 71 should be larger than that of the exhaust opening 73, and the floating ball 71 can block the exhaust opening 73 upwardly.

[0025] Further, a blowout prevention assembly is connected to the exhaust pipe 31. The blowout prevention assembly comprises a first blowout prevention cover 81 and a second blowout prevention cover 82 which are sequentially arranged outside the outlet of the exhaust pipe 31. A plurality of first outlets and second outlets are uniformly formed on the side walls of the first blowout prevention cover 81 and the second blowout prevention cover 82 along the circumferences thereof, respectively. The first outlets and the second outlets are staggered with each other. When the culture solution in the exhaust pipe 31 flows upward, the first blowout prevention cover 81 blocks the upwardly flowing culture solution, slows down the upward impact force of the culture solution, and allows the culture solution to flow out from the first outlets around the first blowout prevention cover 81. The second blowout prevention cover 82 further blocks the culture solution and slows down the impact force thereof.

[0026] Further, the inner top wall of the installation box 3 is provided with a reverse tapered guide groove 33, and the air outlet 73 is connected to the top end of the guide groove 33. The reverse tapered guide groove 33 can more easily collect the air in the installation box 3, which is conducive to emptying the air in the installation box 3, avoiding the air being discharged from the fine filter mechanism when the culture solution passes through the fine filter mechanism, thereby avoiding the generation of bubbles in the purified biological enzyme solution when the culture solution is filtered.

[0027] Those skilled in the art will appreciate that although preferred embodiments of the present application have been described, those skilled in the art, once aware of the basic inventive concept, can make further changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications falling within the scope of the present application. Obviously, those skilled in the art can make various modifications and variations to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the claims of the present application and their equivalents, the present application also intends to include these modifications and variations.

Claims

1. A biocatalyst purification apparatus, characterized by: The utility model provides a filter device, including installation frame (1), be provided with first cylinder (11) on installation frame (1), be provided with rough filter mechanism in first cylinder (11), the top of first cylinder (11) is provided with first liquid inlet pipe (12) to rough filter mechanism is opposite, first liquid inlet pipe (12) is connected with first valve (121), installation box (3) is provided with below first cylinder (11) on installation frame (1), the bottom of first cylinder (11) is connected with first liquid outlet pipe (111), one end of first liquid outlet pipe (111) is connected with installation box (3), the bottom of installation box (3) is connected with blowdown pipe (5), the top of installation box (3) is connected with exhaust pipe (31), the outer wall of first cylinder (11) is connected with air inlet pipe (112), air inlet pipe (112) is connected with gas source, the side wall of installation box (3) is connected with second liquid outlet pipe (32), be provided with fine filter mechanism between installation box (3) and second liquid outlet pipe (32), be provided with valve body in exhaust pipe (31) for emptying the air in installation box (3) and limit solution to enter exhaust pipe (31).

2. The device of claim 1, wherein, The rough filter mechanism comprises a second cylinder (21) rotatably arranged in the first cylinder (11), the top of the second cylinder (21) is open, and the side wall is hollow, a first driving motor (22) is arranged on the installation frame (1), the output shaft of the first driving motor (22) extends into the first cylinder (11) and is fixedly connected coaxially on the bottom wall of the second cylinder (21), and a filter sleeve (23) is fixedly arranged coaxially on the inner wall of the second cylinder (21).

3. The device of claim 2, wherein the device is configured to perform the following steps: The fine filter mechanism comprises a mounting frame (61) and a filter layer (62), the mounting frame (61) is arranged on the side wall of the installation box (3), the mounting frame (61) is provided with a mounting opening penetrating through and communicating the installation box (3) and the second liquid outlet pipe (32), and the filter layer (62) is arranged in the mounting opening. ​ 4. The device of claim 1, wherein the device is configured to purify a biological enzyme. The bottom of the blowdown pipe (5) is detachably connected with a sewage collecting tank (51).

5. The device according to any one of claims 1 to 4, wherein the device is a device for purifying a biological enzyme. The valve body comprises a floating ball (71) and a connecting frame (72), an exhaust port (73) is formed in the installation box (3), the exhaust pipe (31) communicates with the exhaust port (73), the connecting frame (72) is arranged in the installation box (3) and located directly below the exhaust port (73), the floating ball (71) is vertically movably arranged in the connecting frame (72), and the floating ball (71) can block the exhaust port (73) upward.

6. The device of claim 5, wherein the device is configured to perform the following steps: The exhaust pipe (31) is connected with a blowout prevention assembly, the blowout prevention assembly comprises a first blowout prevention cover (81) and a second blowout prevention cover (82) which are sequentially arranged outside the outlet of the exhaust pipe (31), a plurality of first outlets and second outlets are uniformly formed in the side walls of the first blowout prevention cover (81) and the second blowout prevention cover (82) along the circumferences thereof, and the first outlets and the second outlets are staggered with each other. ​ 7. The device of claim 5, wherein the device is configured to perform the following steps: The inner top wall of the installation box (3) is arranged as a guide groove (33) in the shape of an inverted cone, and the exhaust port (73) is connected to the top end of the guide groove (33). ​

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