Bovine serum albumin purification device

Abstract

The utility model relates to a bovine serum albumin purification device, and aims to improve the dropping precision of a salt solution in a protein purification process through automatic design and accurate control. The device comprises a pump box, a conveying wheel set, a liquid conveying rubber pipe, a driving motor and a liquid storage tank. The driving motor drives the conveying wheel set to rotate, and the abutting roller in the conveying wheel set revolves around the main shaft sleeve to extrude the liquid feeding rubber pipe, so that the salt solution is pushed to move along the pipeline, and accurate dripping of the liquid is realized. A groove gland is arranged on one side of the pump box and used for pressing the liquid conveying rubber pipe when the conveying wheel set is not started, and liquid leakage is prevented. The arrangement of the adjusting disc allows the abutting roller to be adjusted in the radial direction so as to adapt to liquid feeding rubber pipes of different specifications, and therefore the output amount of the salt solution is accurately controlled. The device is simple in structure and convenient to operate, can effectively improve the purification efficiency and quality of bovine serum albumin, and is suitable for various experiments and production environments.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biochemistry technique, concretely is a kind of bovine serum albumin purification device. BACKGROUND

[0002] In the purification process of bovine serum albumin, salting-out method is a commonly used method. This method precipitates proteins from solution by gradually increasing the concentration of ammonium sulfate or other salt solutions. However, the traditional salting-out process mainly relies on manual addition of salt solution, and the concentration of the solution is adjusted manually to induce protein precipitation.

[0003] This traditional manual addition method has several significant defects, which directly affect the effect and efficiency of purification. First, it is difficult to accurately control the addition amount and speed of salt solution during manual addition, resulting in uneven solution concentration, which in turn affects the precipitation effect of proteins. This inaccurate control can cause partial over-precipitation or insufficient precipitation of proteins, ultimately affecting the purity and yield of purification. Second, the stability of manual operation is poor, and the addition speed is difficult to maintain constant. This fluctuation easily leads to drastic changes in salt concentration in the solution, which in turn adversely affects the structure and activity of proteins, affecting the repeatability of experiments. In addition, the traditional manual addition method is labor-intensive, requires continuous monitoring and adjustment, and is cumbersome and prone to errors, which makes it difficult to meet the high-precision and high-efficiency requirements in industrial production and high-throughput experiments.

[0004] Therefore, the traditional manual addition method has gradually become difficult to meet the high-standard requirements of modern biochemical research and production for protein purification, and a new device capable of automatically and accurately controlling the addition process of salt solution is urgently needed to improve the efficiency and quality of protein purification. SUMMARY

[0005] The utility model aims at solving the technical problems existing in the prior art or related art.

[0006] Therefore, the purpose of the utility model is to provide a bovine serum albumin purification device, which improves the addition accuracy of salt solution in the protein purification process through automated design and precise control, overcomes the defects of traditional manual addition method, and ensures efficient and high-quality purification of proteins.

[0007] To achieve the above purpose, the utility model provides a bovine serum albumin purification device. The main structure of the device includes pump box, conveying wheel group, liquid feeding rubber tube, driving motor and liquid storage tank.

[0008] The pump box is the core installation platform of the device, used for fixing key components such as the driving motor, the liquid storage tank and the conveying wheel set. The design of the pump box not only ensures the stable installation of these components, but also ensures the efficient transmission and collaborative work between the components. One side of the pump box is provided with a groove cover, through which the liquid feeding rubber tube can be tightly pressed in the internal guide groove of the pump box. This design effectively prevents the automatic flow of the salt solution due to gravity or residual pressure when the conveying wheel set is not started, thereby avoiding liquid waste and unnecessary complication of the system.

[0009] The conveying wheel set is installed in the pump box and directly driven by the driving motor. Its main function is to periodically extrude the liquid feeding rubber tube by driving a plurality of resistance rollers to rotate, so that the salt solution can be accurately dropped at a predetermined speed and amount. The resistance rollers revolve around the main shaft in the conveying wheel set and precisely extrude the rubber tube in each rotation to realize the orderly transmission of the liquid.

[0010] One end of the liquid feeding rubber tube is connected to the liquid storage tank, and the other end extends to the target container. The conveying wheel set gradually transports the salt solution in the liquid storage tank to the target container through the extrusion of the rubber tube by the resistance rollers. The material of the rubber tube is usually silicone or rubber, which has good elasticity and pressure resistance and can restore its shape after a long time of high-frequency extrusion, thereby ensuring the stability of the dropping.

[0011] The driving motor is installed outside the pump box and directly drives the rotation of the conveying wheel set. The speed of the driving motor can be adjusted to control the rotation speed of the resistance rollers and the extrusion frequency of the liquid feeding rubber tube, thereby accurately controlling the dropping speed and amount of the salt solution.

[0012] The liquid storage tank is fixedly installed above the pump box and is mainly used for storing ammonium sulfate or other salt solutions to be dropped. The liquid storage tank is communicated with the liquid feeding rubber tube through the connecting pipeline, so that the salt solution can smoothly enter the liquid feeding rubber tube for transmission under the action of the static pressure in the liquid storage tank.

[0013] Further, the conveying wheel set is designed with an adjustable structure that allows the radial position of the resistance rollers to be finely adjusted. By rotating the adjustment disc, the position of the resistance rollers relative to the liquid feeding rubber tube can be accurately adjusted, thereby changing the amount of liquid transported per rotation. This design allows the output amount of the salt solution to be accurately controlled and optimized by replacing the liquid feeding rubber tube of different specifications or adjusting the position of the resistance rollers according to different experimental conditions or production requirements.

[0014] The beneficial effects achieved by the utility model are as follows:

[0015] 1.The utility model discloses a unique conveying structure is set up, realizes the effective sealing of liquid feeding rubber tube.Under the abutting state of abutting roller and liquid feeding rubber tube surface, the automatic flow of salt solution under the uncontrolled condition can be avoided, and the stability and accuracy of the system are ensured.Meanwhile, when the abutting roller revolves around the main shaft sleeve inside the pump box, the abutting roller extrudes the liquid feeding rubber tube, and the salt solution inside is gradually pushed and automatic output is realized, so that the drop amount and drop speed of salt solution are accurately controlled, and the overall purification efficiency and operation precision are improved.

[0016] 2.The utility model discloses a rotatable adjusting disc and dynamic guide disc structure are set up, and the surface is respectively arranged with arc guide groove and radial guide groove.Under the relative motion of the two, the radial motion of abutting roller can be guided, so that the specific position of abutting roller is adjusted.In addition, by replacing the liquid feeding rubber tube of different inner diameter specifications, the liquid feeding amount of main shaft sleeve in single circle rotation process can be changed.Therefore, the device can more accurately adjust the output amount of salt solution according to actual operation demand, and flexibly adjusts the output efficiency of salt solution under different production demands, meets the diversified experimental and production requirements. DRAWINGS

[0017] Figure 1 It is the overall structure schematic diagram of an embodiment of the utility model;

[0018] Figure 2 It is the pump box surface groove gland opening state schematic diagram of an embodiment of the utility model;

[0019] Figure 3 It is the pump box cross section structure schematic diagram of an embodiment of the utility model;

[0020] Figure 4 It is the conveying wheel group structure schematic diagram of an embodiment of the utility model;

[0021] Figure 5 It is the conveying wheel group exploded structure schematic diagram of an embodiment of the utility model;

[0022] Figure 6 It is the adjusting disc surface structure schematic diagram of an embodiment of the utility model.

[0023] Signs:

[0024] 100, pump box;110, drive motor;120, liquid storage tank;130, groove gland;131, lock catch;

[0025] 200, conveying wheel group;210, main shaft sleeve;220, adjusting disc;230, abutting roller;240, synchronous shaft;211, dynamic guide disc;212, radial guide groove;221, handle;222, arc guide groove;241, damping piece;

[0026] 300, liquid delivery hose; 310, drip head. DETAILED DESCRIPTION

[0027] To make the purpose, technical scheme and advantages of the utility model clearer and more comprehensible, the utility model is further described in detail below in combination with specific embodiments and with reference to the drawings. It should be noted that the embodiments of the utility model and the features in the embodiments can be combined with each other without conflict.

[0028] It is understood that the description is only exemplary and is not intended to limit the scope of the utility model.

[0029] Some embodiments of the utility model provide a bovine serum albumin purification device, which is described below in combination with the drawings.

[0030] In combination with Figures 1-6 As shown in the drawings, the utility model provides a bovine serum albumin purification device, which aims to realize accurate dropwise addition of salt solution through automatic design, so as to improve the purification efficiency and quality of bovine serum albumin. The following describes the components of the device and its working principle in detail.

[0031] The device comprises a pump box 100, a conveying wheel set 200, a liquid delivery hose 300, a driving motor 110 and a liquid storage tank 120. The pump box 100 is the core installation structure of the whole device, which fixes the driving motor 110 and the liquid storage tank 120 and provides support and protection for the installation and operation of the conveying wheel set 200 and the liquid delivery hose 300.

[0032] The pump box 100 is a cuboid structure, and a space for installing the conveying wheel set 200 and accommodating the liquid delivery hose 300 is formed inside the pump box 100. One side of the pump box is provided with an openable slot pressing cover 130, through which the liquid delivery hose 300 can be pressed tightly in the guide slot inside the pump box 100, so as to avoid uncontrolled flow of the salt solution when the conveying wheel set 200 is not started. One end of the slot pressing cover 130 is rotatably connected to the pump box 100 through a hinge, and the other end is provided with a lock catch 131, which cooperates with the pump box 100 to ensure the stability of the slot pressing cover 130 in the working state.

[0033] The conveying wheel set 200 is installed inside the pump box 100 and is driven to rotate by the driving motor 110 through a synchronous shaft 240. The conveying wheel set 200 comprises a main shaft sleeve 210, an adjusting disc 220 and a plurality of abutting rollers 230. The conveying wheel set 200 is fixed to the output end of the driving motor 110 and is rotatably installed on the inner side of the driving motor 110. The adjusting disc 220 is fixedly connected to the synchronous shaft 240, and through the rotation of the synchronous shaft 240, the adjusting disc 220 and the abutting rollers 230 are driven to revolve around the main shaft sleeve 210. The abutting rollers 230 are installed between the adjusting disc 220 and the dynamic guide disc 211 and can be radially adjusted in the relative movement of the two.

[0034] The surface of the synchronous shaft 240 is provided with a damping piece 241 which contacts the inner wall of the main shaft sleeve 210 by friction to limit the free rotation of the synchronous shaft 240, thereby stabilizing the movement of the adjustment disc 220 during the radial adjustment process and preventing the shaking caused by inertia from affecting the position accuracy of the abutting roller 230.

[0035] The abutting roller 230 contacts the liquid delivery rubber tube 300 and delivers the liquid by extruding the liquid delivery rubber tube 300 during the revolution. The adjustment disc 220 and the dynamic guide disc 211 are provided with an arc guide groove 222 and a radial guide groove 212, which enable the abutting roller 230 to move radially when the main shaft sleeve 210 rotates, thereby adjusting the contact position of the abutting roller 230 with the liquid delivery rubber tube 300 and changing the liquid delivery amount per revolution.

[0036] The abutting rollers 230 are evenly distributed along the circumferential direction of the synchronous shaft 240, i.e., the angular interval of each abutting roller in the circumferential direction is equal. This structure ensures that the rubber tube is extruded uniformly and continuously, avoids the instability of liquid flow, and improves the continuity and accuracy of liquid delivery.

[0037] The liquid delivery rubber tube 300 is made of flexible material, such as silicone or rubber, and has good elasticity and pressure resistance. One end of the liquid delivery rubber tube 300 is connected to the liquid storage tank 120 through a pipeline, and the other end is connected to the liquid drop head 310. The liquid delivery rubber tube 300 is arranged around half of the outer periphery of the delivery wheel set 200, and part of the rubber tube is arranged in the guide groove of the groove cover 130. The liquid delivery rubber tube 300 is periodically extruded by the abutting roller 230 of the delivery wheel set 200, and the salt solution is gradually pushed forward, thereby realizing the orderly dropwise addition of the liquid.

[0038] The liquid delivery rubber tube 300 is arranged in a U-shaped path around the outer periphery of the delivery wheel set 200, and the arc segment of the U-shaped path abuts against the arc groove in the groove cover 130, forming a stable bending segment structure, which helps to improve the liquid pushing efficiency during the extrusion process.

[0039] The output end of the driving motor 110 is fixedly connected to one end of the main shaft sleeve 210, and the synchronous shaft 240 is rotatably sleeved on the inner side of the main shaft sleeve 210. The main shaft sleeve 210, the dynamic guide disc 211, the synchronous shaft 240, and the adjustment disc 220 are arranged along the same axis to form a coaxial structure layout, which ensures that the delivery wheel set 200 remains stable and force-symmetrical during rotation.

[0040] When the driving motor 110 is not turned on, the groove cover 130 presses the liquid delivery rubber tube 300 tightly in the pump box 100 to prevent the solution from flowing out unintentionally. When the driving motor 110 is started, the abutting roller 230 revolves with the delivery wheel set 200 to extrude the liquid delivery rubber tube 300, thereby accurately controlling the dropwise addition amount of the salt solution.

[0041] The driving motor 110 is installed outside the pump box 100 and connected with the conveying wheel set 200 through the synchronous shaft 240. The driving motor 110 provides rotary power to make the conveying wheel set 200 rotate around the main shaft sleeve 210, and then drive the pressing roller 230 to extrude the liquid conveying rubber tube 300. The rotating speed of the driving motor 110 can be adjusted, and the user can set a suitable rotating speed according to actual needs, so as to control the dropping speed and amount of the salt solution.

[0042] The liquid storage tank 120 is fixed on the top or side of the pump box 100, and is used to store ammonium sulfate or other salt solutions to be dropped. The liquid storage tank 120 is communicated with the liquid conveying rubber tube 300 through the connecting pipeline, and under the action of gravity or weak pressure, the salt solution is conveyed to the liquid conveying rubber tube 300, ready for precise dropping operation. The bottom of the liquid storage tank 120 is provided with a liquid outlet end, which is communicated with the input end of the liquid conveying rubber tube 300 through a soft connecting pipe, and is used to transmit the salt solution in the liquid storage tank 120 to the inside of the liquid conveying rubber tube 300. The pipe diameter of the liquid outlet end matches the interface of the liquid conveying rubber tube 300, ensuring reliable sealing and no leakage during liquid transmission.

[0043] Operation and adjustment

[0044] When the driving motor 110 starts, the conveying wheel set 200 begins to rotate, the pressing roller 230 extrudes the liquid conveying rubber tube 300, and the salt solution is gradually pushed along the internal channel of the rubber tube, and finally drops out from the liquid drop head 310. The user can control the dropping speed of the solution by adjusting the rotating speed of the driving motor 110. In addition, by rotating the adjusting disc 220, the radial position of the pressing roller 230 can be adjusted, so as to change the liquid conveying amount in a single rotation.

[0045] The outer side of the adjusting disc 220 is provided with a handle 221, which can be manually rotated by the user to make the adjusting disc 220 produce a deflection motion around the synchronous shaft 240 relative to the main shaft sleeve 210. The deflection motion guides the pressing roller 230 to move radially inward or outward through the relative sliding of the arc guide groove 222 and the radial guide groove 212, so as to adjust the pressing degree of the liquid conveying rubber tube 300, and realize fine control of the liquid conveying amount.

[0046] When it is necessary to replace the liquid conveying rubber tube 300 to adapt to different experimental needs, the operation can be performed by unlocking the lock buckle 131 and opening the groove gland 130. After replacement, the rubber tube is placed in the pump box 100 again, and the groove gland 130 is closed to restore the normal working state. The inner side of the groove gland 130 is provided with an arc groove, which is matched with the arc profile of the liquid conveying rubber tube 300 when it is bent on the outer periphery of the conveying wheel set 200, so as to tightly press the rubber tube, preventing liquid leakage due to pressure fluctuation.

[0047] The arc-shaped guide groove 222 is an arc-shaped sliding groove distributed along the circumferential direction of the adjusting disc 220, used to guide the micro-adjustment of the abutting roller 230 in the radial direction.

[0048] The number of the radial guide grooves 212 and the arc-shaped guide grooves 222 is the same as and one-to-one corresponding to the number of the abutting rollers 230.

[0049] Application scenario: The device is suitable for the purification process of bovine serum albumin. By accurately controlling the drop amount and speed of the salt solution, the protein is precipitated under the best conditions, and the efficiency and quality of purification are improved. At the same time, the device can also be used in other biochemical experiments and production processes that require accurate liquid transmission, and has wide application prospects.

[0050] Working principle and use process of the utility model:

[0051] The core working principle of the bovine serum albumin purification device is based on the operation mode of the peristaltic pump. The device drives the conveying wheel set 200 to rotate through the driving motor 110, so that the plurality of abutting rollers 230 revolve along the outer periphery of the liquid conveying rubber tube 300, gradually extruding the flexible liquid conveying rubber tube 300, thereby pushing the salt solution to move along the tube and finally drop out. This way can accurately control the flow and flow rate of the liquid, and ensure that the drop amount and speed of the salt solution in the protein purification process reach the optimum.

[0052] Specifically, when the driving motor 110 starts, the conveying wheel set 200 drives the abutting roller 230 to rotate around the main shaft sleeve 210. With the revolution of the abutting roller 230, the liquid conveying rubber tube 300 is gradually extruded at different positions, thereby generating a continuous pushing force to send the salt solution from the liquid storage tank 120 to the target container. During the conveying process, the sliding contact between the abutting roller 230 and the surface of the liquid conveying rubber tube 300 ensures the uniform flow of the liquid, while preventing backflow or leakage of the liquid. The radial adjustment function of the adjusting disc 220 allows users to change the extrusion force of the abutting roller 230 on the liquid conveying rubber tube 300 according to actual needs, thereby controlling the delivery amount of the liquid.

[0053] Use process

[0054] Device preparation: First, install the components of the bovine serum albumin purification device together to ensure that the pump box 100, the driving motor 110, the conveying wheel set 200, the liquid conveying rubber tube 300 and the liquid storage tank 120 are connected stably.

[0055] Confirm that the liquid storage tank 120 has been filled with ammonium sulfate or other salt solution of the required concentration.

[0056] Check whether the specifications of the liquid conveying rubber tube 300 match the experimental requirements. If replacement is required, it can be operated by opening the groove pressure cover 130.

[0057] Start the device: Close the tank cover 130 and ensure it is locked in place, and make the liquid delivery tube 300 sealed in the state of abutting between the tank cover 130 and the abutting roller 230 to prevent liquid leakage.

[0058] Turn on the power and start the drive motor 110 to begin the rotation of the conveying wheel set 200.

[0059] The conveying wheel set 200 drives the abutting roller 230 to revolve, gradually extruding the liquid delivery tube 300, making the salt solution move along the pipeline, preparing for the dripping stage.

[0060] Adjust the dripping speed and amount:

[0061] According to the experimental requirements, adjust the rotation speed of the conveying wheel set 200 by adjusting the speed of the drive motor 110, so as to adjust the dripping speed of the salt solution.

[0062] If precise adjustment of the dripping amount is needed, the radial position of the abutting roller 230 can be changed by rotating the adjusting disc 220 to adjust the extrusion force on the liquid delivery tube 300.

[0063] By observing the value of the flowmeter without the marked number, confirm whether the dripping amount meets the expected requirements, and further adjust as needed.

[0064] Monitoring and adjustment: During the liquid dripping process, monitor the changes of the flowmeter and the experimental solution to ensure that the dripping speed and amount remain stable.

[0065] If any abnormalities are found, such as poor liquid flow or abnormal dripping speed, the motor can be stopped, the position of the abutting roller 230 or the liquid delivery tube 300 can be adjusted, or a different specification of the liquid delivery tube 300 can be replaced.

[0066] Stop and clean up: After the purification process is completed, turn off the drive motor 110 and disconnect the power to stop the rotation of the conveying wheel set 200.

[0067] Open the tank cover 130, take out the liquid delivery tube 300 and clean or replace it, and clean up the residual solution in the liquid storage tank 120.

[0068] Clean and maintain the entire device to ensure it is in good condition for next use.

[0069] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "a specific embodiment", and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily mean the same embodiment or example. Furthermore, the described specific features, structures, materials or characteristics can be combined in any one or more embodiments or examples in a suitable manner.

[0070] Although the embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the claims and their equivalents.

Claims

1. A bovine serum protein purification apparatus, characterized by, Include: Pump box (100), conveying wheel group (200) and can be arbitrarily replaced different diameter specifications of liquid sending rubber tube (300) and fixed on the surface of the pump box (100) drive motor (110) and liquid storage tank (120), the conveying wheel group (200) is fixed on the output end of the drive motor (110) and is rotatably installed on the inside of the drive motor (110), one side of the pump box (100) is provided with a groove gland (130), one side of the groove gland (130) is provided with an arc groove matched with the conveying wheel group (200), the liquid sending rubber tube (300) is detachably arranged on the inside of the pump box (100), one end of the liquid sending rubber tube (300) is communicated with the liquid outlet end of the liquid storage tank (120), and the other end is wound around the outer periphery of the conveying wheel group (200) and then penetrates out from the surface of the pump box (100), the other end of the liquid sending rubber tube (300) is fixedly connected with a liquid drop head (310), the conveying wheel group (200) comprises a main shaft sleeve (210), an adjusting disc (220) and a plurality of abutting rollers (230), one side of the adjusting disc (220) is fixedly connected with a synchronous shaft (240), one end of the synchronous shaft (240) is rotatably sleeved on the inside of the main shaft sleeve (210), the surface of the synchronous shaft (240) is provided with a damping piece (241) frictionally abutting on the inside of the main shaft sleeve (210), the surface of the main shaft sleeve (210) is fixedly installed with a dynamic guide disc (211), the opposite surfaces of the dynamic guide disc (211) and the adjusting disc (220) are respectively provided with a radial guide groove (212) and an arc guide groove (222), a plurality of the abutting rollers (230) are uniformly distributed in the circumferential direction of the outer periphery of the synchronous shaft (240) and located between the dynamic guide disc (211) and the adjusting disc (220), both ends of the abutting roller (230) are slidably installed on the inside of the dynamic guide disc (211) and the arc guide groove (222), the abutting roller (230) is in abutment with the surface of the liquid sending rubber tube (300) under the drive motor (110) to slide along the surface of the liquid sending rubber tube (300) to form a liquid conveying effect.

2. A device for purifying bovine serum proteins according to claim 1, characterized in that The surface of the adjusting disc (220) is provided with a handle (221), which is convenient for manually operating the deflection movement of the adjusting disc (220) relative to the main shaft sleeve (210).

3. The apparatus for purifying bovine serum proteins according to claim 1, wherein The radial guide groove (212) is arranged in the radial direction, the arc guide groove (222) is an arc-shaped sliding groove, the number of the radial guide groove (212) and the arc guide groove (222) is the same as that of the abutting roller (230) and corresponds one by one.

4. The apparatus for purifying bovine serum proteins according to claim 1, wherein The liquid sending rubber tube (300) is a silica gel or rubber hose structure, the liquid sending rubber tube (300) is arranged in the outer periphery of the conveying wheel group (200) in a U shape, and the arc segment of the liquid sending rubber tube (300) is in abutment with the arc groove surface of the groove gland (130).

5. The apparatus for purifying bovine serum proteins according to claim 1, wherein The output end of the drive motor (110) is fixedly connected with one end of the main shaft sleeve (210), the synchronous shaft (240) is rotatably sleeved on the inside of the main shaft sleeve (210), the main shaft sleeve (210), the dynamic guide disc (211), the synchronous shaft (240) and the adjusting disc (220) are coaxially arranged.

6. The apparatus for purifying bovine serum proteins according to claim 1, wherein The surface of the drop head (310) is provided with a flow meter for monitoring the output of the salt solution.

Images