High-efficiency low-consumption dissolved oxygen fermentation tank

Abstract

A high-efficiency low-consumption dissolved oxygen fermentation tank belongs to the technical field of microbial fermentation equipment and comprises a tank body, a tank cover, a condenser, a motor and an air distributor, a transmission shaft, a plurality of six-straight-blade turbine stirrers and a rake type defoaming device are arranged in the tank body, and the rake type defoaming device and the six-straight-blade turbine stirrers are arranged on the transmission shaft in a penetrating and sleeving manner; the tank body is of a double-layer structure; a plurality of air inlets, a defoaming electrode and a temperature sleeve are arranged on the tank cover; the air distributor comprises a plurality of air pipes, two baffles and an air distribution disc, and the air distribution disc is fixedly arranged at the bottom of the tank body; the oxygen supply device is simple in structure, and the oxygen supply area and the oxygen supply amount are increased by adopting the air distribution disc; the air pipes and the baffles are uniformly distributed, so that the same effect as the baffles can be achieved; the baffle and the lower side of the air pipe are matched with the stirrer to scatter bubbles, and the purpose of increasing dissolved oxygen is achieved through matching of the two functions; and the rake type defoaming device and the defoaming electrode are matched for defoaming twice, so that the defoaming effect is more remarkable.

Description

Technical Field

[0001] This utility model belongs to the technical field of microbial fermentation equipment, and specifically relates to a high-efficiency, low-consumption dissolved oxygen fermenter. Background Technology

[0002] A microbial fermenter is a device used for microbial fermentation production. Its main function is to provide suitable environmental conditions for microbial fermentation. These include temperature, dissolved oxygen levels, culture medium concentration, and fermentation product concentration, all of which affect the fermentation process. Dissolved oxygen levels, in particular, have a significant impact on the fermentation of microorganisms, especially aerobic bacteria. Therefore, dissolved oxygen levels are crucial for fermenters, and the effectiveness of the corresponding dissolved oxygen structure, i.e., the air distributor, is also essential.

[0003] Currently, most fermenters use annular air distributors, which consist of an air pipe connected to a perforated ring. The air pipe connects to an air inlet, and sterile air enters through the inlet, passes through the air pipe, and exits through the small holes in the air distribution ring. An agitator and baffles break up the air bubbles, thus achieving dissolved oxygen. However, sterile air only exits through the ring, leaving no oxygen supply in the center, resulting in uneven oxygenation and low dissolved oxygen efficiency, which is detrimental to microbial fermentation. Furthermore, due to the low oxygen supply efficiency, annular air distributors require continuous oxygen supply, leading to higher energy consumption. Summary of the Invention

[0004] The purpose of this invention is to solve the problems of low dissolved oxygen supply efficiency and high energy consumption in existing fermenters, and to provide a high-efficiency, low-energy-consumption dissolved oxygen fermenter.

[0005] A high-efficiency, low-consumption dissolved oxygen fermenter includes a tank body, a tank cover, a condenser, a motor, and an air distributor; wherein the tank cover is mounted on the tank body via an upper flange and a lower flange, the condenser and the motor are respectively mounted on the tank cover, and the air distributor is located inside the tank body;

[0006] The tank is equipped with a drive shaft, several six-blade turbine agitators and a rake-type defoamer. The drive shaft passes through the tank cover and is connected to the output end of the motor. The rake-type defoamer and several six-blade turbine agitators are sleeved on the drive shaft. The tank has a double-layer structure with a condensate outlet at the top and a condensate inlet at the bottom.

[0007] The can lid is provided with several air inlets, a material filling port, and an inoculation port; the can lid is also equipped with a defoaming electrode and a temperature sleeve.

[0008] The air distributor includes several air pipes, two baffles, and an air distribution disc, wherein the number of air pipes is equal to the number of air inlets; the air distribution disc is fixedly installed at the bottom of the tank, the air pipes are evenly distributed on the air distribution disc and connected to the air distribution disc, the baffles are symmetrically fixedly installed on the air distribution disc; the other end of the air pipe is connected to the air inlet.

[0009] The aforementioned six-blade turbine agitator consists of two units.

[0010] There are two air inlets and two air pipes.

[0011] The tank body is equipped with four support rods at the bottom.

[0012] The rake-type defoamer and the six-blade turbine agitator can be freely adjusted in position on the drive shaft and then fixed.

[0013] The working process and working principle of this utility model:

[0014] The tank has a double-layer structure. Cooling water is injected into the fermentation tank through the cooling water inlet and discharged through the cooling water outlet, thereby cooling the fermentation liquid in the fermentation tank.

[0015] The six-bladed turbine agitator rotates in conjunction with a drive shaft powered by an electric motor. A rake-type defoamer mounted on the drive shaft and a defoaming electrode mounted on the tank lid work together to achieve better defoaming results.

[0016] The air pipes and baffles are evenly distributed and can serve the same function as the baffles.

[0017] In using this invention, sterile air is introduced into the fermentation broth through the air inlet, air pipe, and air distribution disc, forming sterile bubbles. Simultaneously, the drive shaft rotates under the drive of a motor, and a six-bladed turbine agitator mounted on the drive shaft rotates, stirring and dispersing the sterile bubbles discharged from the air distribution disc to achieve oxygenation. Excess air is condensed and discharged through a condenser. The foam generated in this process is defoamed for the first stage by a rake-type defoamer, and then defoamed a second time by a defoaming electrode on the tank lid. The working principle is that the gas generated by electrolysis forms tiny bubbles on the electrode surface. These bubbles increase the surface area of ​​the liquid, enhancing its surface tension, making the bubbles easier to break and expel. A temperature sleeve on the tank lid measures the temperature inside the tank. When the temperature becomes too high during fermentation, condensate is injected into the tank's jacket to lower the temperature. The heated condensate is discharged through the condensate outlet, thus cooling the fermentation broth in the tank.

[0018] The beneficial effects of this utility model are:

[0019] This invention has a simple structure. It uses an air distribution disc instead of a ring to increase the oxygen supply area and oxygen supply. The air pipes and baffles are evenly distributed and can play the same role as the baffles. The baffles and the lower side of the air pipes work with the stirrer to break up the bubbles. The combination of the two functions achieves the purpose of increasing dissolved oxygen. The rake-type defoamer and the defoaming electrode are used together to perform two defoaming processes, making the defoaming effect more significant. Attached Figure Description

[0020] Figure 1 This is a cross-sectional view of the tank body and tank lid of an embodiment of the present utility model.

[0021] Figure 2 This is a rear view of the external structure of an embodiment of the present utility model.

[0022] Figure 3 This is a schematic diagram of the can lid surface according to an embodiment of the present invention.

[0023] Figure 4 This is a schematic diagram of the air distributor structure according to an embodiment of the present utility model.

[0024] Figure 5 This is a rear view of the can lid and the internal structure of the can according to an embodiment of the present utility model.

[0025] Figure 6 A comparison chart of air volume before and after adopting this invention for the microbial fermentation production of tryptophan.

[0026] Figure 7 A comparison chart of rotation speeds before and after adopting this invention for the microbial fermentation production of tryptophan.

[0027] Figure 8 A comparative data chart showing the OD660 values ​​before and after using this invention for the microbial fermentation production of tryptophan. Figure 9 A comparative data chart showing the tryptophan yield before and after adopting this invention for microbial fermentation production of tryptophan.

[0028] Figure 10 A graph showing data on the production of guanosine by microbial fermentation. Detailed Implementation

[0029] Please see Figures 1 to 6 The image shown is an embodiment of this utility model.

[0030] A high-efficiency, low-consumption dissolved oxygen fermenter includes a tank body 1, a tank cover 2, a condenser 3, a motor 4, and an air distributor 5; wherein the tank cover 2 is mounted on the tank body 1 via an upper flange 21 and a lower flange 22, the condenser 3 and the motor 4 are respectively mounted on the tank cover 2, and the air distributor 5 is located inside the tank body 1.

[0031] The tank body 1 is equipped with a drive shaft 11, two six-blade turbine agitators 12 and a rake-type defoamer 13. The drive shaft 11 passes through the tank cover 2 and is connected to the output end of the motor 4. The rake-type defoamer 13 and the two six-blade turbine agitators 12 are sleeved on the drive shaft 11. The tank body 1 has a double-layer structure, with a condensate outlet 14 at the top and a condensate inlet 15 at the bottom.

[0032] The can lid 2 is provided with two air inlets 23, a material filling port 24 and an inoculation port 25; the can lid 2 is provided with a defoaming electrode 26 and a temperature sleeve 27;

[0033] The air distributor 5 includes two air pipes 51, two baffles 52, and an air distribution disc 53. The air distribution disc 53 is fixedly installed at the bottom of the tank body 1. The air pipes 51 are evenly distributed on the air distribution disc 53 and are connected to the air distribution disc 53. The baffles 52 are symmetrically fixedly installed on the air distribution disc 53. The other end of the air pipe 51 is connected to the air inlet 23.

[0034] The bottom of the tank body 1 is provided with four support rods 16.

[0035] The rake-type defoamer 13 and the six-blade turbine agitator 12 can be freely adjusted in position on the drive shaft 11 and then fixed.

[0036] The working principle and process of this embodiment:

[0037] Tank 1 has a double-layer structure. Cooling water is injected into the fermentation tank through the cooling water inlet 15 and the heated cooling water is discharged through the cooling water outlet 14, thereby cooling the fermentation liquid in the fermentation tank.

[0038] Two six-bladed turbine agitators 12 rotate in conjunction with a drive shaft 11 powered by a motor 4. A rake-type defoamer 13 mounted on the drive shaft 11 and a defoaming electrode 26 mounted on the tank lid 2 work together to achieve a better defoaming effect.

[0039] The air pipe 51 and the baffle 52 are evenly distributed and can play the same role as the baffle 52.

[0040] In this embodiment, sterile air is discharged into the fermentation broth through the air inlet 23, air pipe 51, and air distribution disc 53 to form sterile bubbles. Simultaneously, the drive shaft 11 rotates under the drive of the motor 4, and two six-bladed turbine agitators 12 mounted on the drive shaft 11 rotate, stirring and dispersing the sterile bubbles discharged from the air distribution disc 53 to achieve dissolved oxygen. Excess air is condensed and discharged through the condenser 3. The fermentation broth foam generated in this process is defoamed for the first stage by the rake-type defoamer 13, which rotates with the liquid. A secondary defoaming process is performed by the defoaming electrode 26 mounted on the tank lid 2. The working principle is that the gas generated by electrolysis forms tiny bubbles on the electrode surface. These bubbles increase the surface area of ​​the liquid, enhance the surface tension of the liquid, and make the bubbles easier to break and eliminate. The temperature sleeve 27 installed on the tank cover 2 is used to measure the temperature inside the tank 1. When the temperature is too high during the fermentation process, the temperature inside the fermentation tank is reduced by injecting condensate into the jacket of the tank 1. The condensate with the increased temperature is discharged through the condensate outlet 14, thereby cooling the fermentation liquid in the fermentation tank.

[0041] See Figures 6 to 9 By recording and comparing various values ​​before and after using the air distribution disk 53 in the fermenter to produce tryptophan through microbial fermentation, it can be concluded that the effect of the fermenter is greatly improved after using the air distribution disk 53.

[0042] Please see Figure 10 After the fermenter adopts the air distribution disk 53, guanosine is produced by microbial fermentation, and various values ​​are recorded.

Claims

1. A high-efficiency, low-consumption dissolved oxygen fermentation tank, characterized in that: It includes a tank body (1), a tank cover (2), a condenser (3), a motor (4), and an air distributor (5); wherein the tank cover (2) is mounted on the tank body (1) via an upper flange (21) and a lower flange (22), the condenser (3) and the motor (4) are mounted on the tank cover (2), and the air distributor (5) is mounted inside the tank body (1); The tank (1) is equipped with a drive shaft (11), several six-bladed turbine agitators (12) and a rake-type defoamer (13). The drive shaft (11) passes through the tank cover (2) and is connected to the output end of the motor (4). The rake-type defoamer (13) and several six-bladed turbine agitators (12) are sleeved on the drive shaft (11). The tank (1) has a double-layer structure with a condensate outlet (14) at the top and a condensate inlet (15) at the bottom. The can lid (2) is provided with several air inlets (23), feeding inlets (24) and inoculation inlets (25); the can lid (2) is provided with defoaming electrodes (26) and temperature sleeves (27); The air distributor (5) includes several air pipes (51), two baffles (52) and an air distribution disc (53), wherein the number of air pipes (51) is equal to the number of air inlets (23); the air distribution disc (53) is fixedly installed at the bottom of the tank (1), the air pipes (51) are evenly distributed on the air distribution disc (53) and connected to the air distribution disc (53), and the baffles (52) are symmetrically fixedly installed on the air distribution disc (53); the other end of the air pipe (51) is connected to the air inlet (23).

2. The high-efficiency, low-consumption dissolved oxygen fermenter according to claim 1, characterized in that: The six-blade turbine agitator (12) consists of two units.

3. The high-efficiency, low-consumption dissolved oxygen fermenter according to claim 1, characterized in that: There are two air inlets (23) and two air pipes (51).

4. The high-efficiency, low-consumption dissolved oxygen fermenter according to claim 1, characterized in that: The tank (1) is provided with four support rods (16) at the bottom.

5. The high-efficiency, low-consumption dissolved oxygen fermenter according to claim 1, characterized in that: The rake-type defoamer (13) and the six-blade turbine agitator (12) can be fixed after their positions are freely adjusted on the drive shaft (11).

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