Culture device for three-dimensional cells

Abstract

The utility model relates to the technical field of cell culture, and discloses a culture device for three-dimensional cells, which comprises a culture solution device body, the bottom of the culture solution device body is fixedly connected with a conical hopper, and a second support frame is fixedly connected between the inner walls of the culture solution device body. The top of the second supporting frame penetrates through and is slidably connected with a square block, the top of the square block is fixedly connected with a supporting rod, the outer circle of the supporting rod is fixedly connected with evenly-distributed connecting rings, the tops of the connecting rings are fixedly connected with evenly-distributed guide rails, and a guide plate is slidably connected between every two adjacent guide rails. And connecting plates are fixedly connected to one sides of every two adjacent guide plates. According to the culture solution device, the cover is taken down through rotation of the guide rails, the limiting holes, the square blocks and the culture units, then the supporting rods are pulled upwards to enable the square blocks to be separated from the second supporting frame, and then the supporting rods are taken out of the culture solution device body.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to a device for culturing three-dimensional cells. Background Technology

[0002] Cell culture, as the cornerstone of biological science, is the foundation of various biological experimental research and a crucial area of ​​technical research. Three-dimensional culture is an important branch of this field. Unlike two-dimensional cell culture, three-dimensional cell culture requires higher standards of three-dimensional culture equipment due to the different types of cells, carriers, and the construction of different culture systems. The selection of the type of culture equipment is also more demanding.

[0003] Existing culture devices mostly employ two-dimensional cell culture, which typically involves growth along a two-dimensional plane on the surface of a culture flask made of glass or plastic.

[0004] However, in current culture devices, the culture units are fragile and difficult to replace when damaged, which affects cell culture. To address these issues, a culture device for three-dimensional cells is proposed. Utility Model Content

[0005] The purpose of this invention is to provide a three-dimensional cell culture device, which solves the problem in the prior art that the culture unit is a fragile item and is inconvenient to replace when damaged, thus affecting cell culture.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a culture device for three-dimensional cells, comprising a culture solution container body, a conical hopper fixedly connected to the bottom of the culture solution container body, a second support frame fixedly connected between the inner walls of the culture solution container body, a square block slidably connected through the top of the second support frame, a support rod fixedly connected to the top of the square block, a uniformly distributed connecting ring fixedly connected to the outer ring of the support rod, a uniformly distributed guide rail fixedly connected to the top of the connecting ring, a guide plate slidably connected between adjacent guide rails, a connecting plate fixedly connected to one side of adjacent guide plates, a culture unit fixedly connected to the top of adjacent connecting plates, a limiting hole opened on one side of adjacent guide rails, a limiting groove opened on one side of adjacent guide plates, a spring fixedly connected to one side of the inner wall of adjacent limiting grooves, a limiting ball fixedly connected to one side of adjacent springs, a fine-pore grid fixedly connected to one side of the culture unit, and a conveying component provided on one side of the outer ring of the culture solution container body.

[0007] By adopting the above technical solution, pulling the culture unit that needs to be replaced will allow the limiting ball to slide out of the limiting hole, and then the culture unit can be removed for replacement. When installing, the guide plate is placed into the guide rail and slid. When the limiting ball moves to the position of the limiting hole, the spring is no longer under force and rebounds, and the limiting ball will be locked into the limiting hole for limiting, thus completing the installation.

[0008] As a further description of the above technical solution: the delivery assembly includes an inlet pipe that passes through and is fixedly connected to the body of the culture solution container. One end of the inlet pipe passes through and is fixedly connected to a first transport pipe. The other end of the first transport pipe passes through and is provided with a rotary joint. The right end of the inlet pipe passes through and is fixedly connected to a second solenoid valve. The inner ring of the rotary joint is provided with a second transport pipe. The inner ring of the support rod passes through and is provided with a conduit. The outer ring of the conduit passes through and is fixedly connected to a uniformly distributed first outlet pipe. The other end of the first outlet pipe is fixedly connected to a support rod, and the first outlet pipe passes through and extends to one side of the support rod.

[0009] By adopting the above technical solution, the delivery component is mainly used to deliver the cell hydrogel system.

[0010] As a further description of the above technical solution: a second liquid outlet pipe is connected through and fixedly connected to the bottom of the conical hopper, and a first solenoid valve is connected through and fixedly connected to the bottom of the second liquid outlet pipe.

[0011] By adopting the above technical solution, the conical hopper prevents waste liquid from leaving residue during discharge.

[0012] As a further description of the above technical solution: a first handle is fixedly connected to one side of the culture unit.

[0013] By adopting the above technical solution, the person in charge can easily move the culture unit.

[0014] As a further description of the above technical solution: the limiting hole and the limiting ball are engaged.

[0015] By adopting the above technical solution, the culture unit is installed when the limiting ball and the limiting hole are engaged.

[0016] As a further description of the above technical solution: the outer ring of the culture solution container body has two air inlet pipes that are connected through and fixedly connected, and the outer ring of the two air inlet pipes is threaded with a filter screen. The bottom of the outer ring of the culture solution container body is provided with a first support frame.

[0017] By adopting the above technical solution, the first support frame supports the body of the culture solution container.

[0018] As a further description of the above technical solution: the top of the outer ring of the culture solution container is threaded with a cap, and a second handle is fixedly connected to the top of the cap.

[0019] By adopting the above technical solution, the second handle can easily rotate the lid.

[0020] As a further description of the above technical solution: a second transport pipe is connected through and fixedly connected to one side of the outer ring of the support rod, and a conduit is connected through and fixedly connected to one end of the second transport pipe.

[0021] By adopting the above technical solution, the second transport pipe transports the liquid.

[0022] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0023] This utility model provides a three-dimensional cell culture device. It consists of a guide rail, a limiting hole, a square block, and a culture unit. The cover is removed by rotating it, and then the support rod is pulled upwards to separate the square block from the second support frame. The support rod is then removed from the culture solution container. Pulling the first handle on the culture unit to be replaced allows the limiting ball to slide out of the limiting hole, and the culture unit can then be removed for replacement. During installation, the guide plate is placed into the guide rail and slid. When the limiting ball moves to the limiting hole position, the spring is no longer under force and rebounds, causing the limiting ball to lock into the limiting hole for positioning, thus completing the installation. This device facilitates installation and disassembly, makes replacement convenient, and improves the overall performance. Attached Figure Description

[0024] Figure 1 This is a three-dimensional view of the overall structure of this utility model;

[0025] Figure 2 This is a cross-sectional view of the overall structure of this utility model;

[0026] Figure 3 This is an exploded view of the square block structure of this utility model;

[0027] Figure 4 This is an exploded view of the culture unit structure of this utility model;

[0028] Figure 5 This is a schematic diagram of the guide plate structure of this utility model;

[0029] Figure 6 This is a schematic diagram of the support rod structure of this utility model.

[0030] In the diagram: 1. Culture solution container body; 2. Lid; 3. First support frame; 4. Conical hopper; 5. Liquid inlet pipe; 6. Air inlet pipe; 7. Filter screen; 8. Support rod; 9. Guide tube; 10. Second transport pipe; 11. First transport pipe; 12. Rotary joint; 13. First liquid outlet pipe; 14. Square block; 15. Second support frame; 16. Connecting ring; 17. Culture unit; 18. Guide rail; 19. Limiting hole; 20. Guide plate; 21. Limiting groove; 22. Spring; 23. Limiting ball; 24. First handle; 25. Connecting plate; 26. Fine-pore grid; 27. Second liquid outlet pipe; 28. First solenoid valve; 29. ​​Second solenoid valve; 30. Second handle. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] To further understand the content of this utility model, a detailed description of this utility model will be provided in conjunction with the accompanying drawings.

[0033] Combination Figure 1 This utility model discloses a three-dimensional cell culture device, comprising a culture solution container body 1, a conical hopper 4 fixedly connected to the bottom of the culture solution container body 1 to facilitate the discharge of waste liquid without accumulation, a second support frame 15 fixedly connected to the inner wall of the culture solution container body 1, a second outlet pipe 27 penetrating and fixedly connected to the bottom of the conical hopper 4, the second support frame 15 supporting the support rod 8 as a whole and facilitating its removal, a first solenoid valve 28 penetrating and fixedly connected to the bottom of the second outlet pipe 27, a first handle 24 fixedly connected to one side of the culture unit 17, pulling the first handle 24 to facilitate the replacement of the culture unit 17, a limiting hole 19 engaging with a limiting ball 23, and the outer side of the culture solution container body 1... Two air inlet pipes 6 are connected and fixedly connected through the ring. A filter screen 7 is placed on the air inlet pipe 6 to reduce the entry of impurities. The filter screen 7 can be replaced by rotating it. The outer ring of both air inlet pipes 6 is threaded with a filter screen 7. A first support frame 3 is set at the bottom of the outer ring of the culture solution container body 1. The culture solution container body 1 is placed in the first support frame 3 and serves as support. A cover 2 is threadedly connected to the top of the outer ring of the culture solution container body 1. A second handle 30 is fixedly connected to the top of the cover 2. Rotating the second handle 30 will rotate the cover 2 and remove it. A second transport pipe 10 is connected and fixedly connected through one side of the outer ring of the support rod 8. A conduit 9 is connected and fixedly connected to one end of the second transport pipe 10.

[0034] Combination Figure 2 - Figure 5 A square block 14 is slidably connected through the top of the second support frame 15. A support rod 8 is fixedly connected to the top of the square block 14. Evenly distributed connecting rings 16 are fixedly connected to the outer ring of the support rod 8. The connecting rings 16 are connected to the support rod 8. Evenly distributed guide rails 18 are fixedly connected to the top of the connecting rings 16. A guide plate 20 is slidably connected between each two adjacent guide rails 18. The guide plate 20 moves within the guide rail 18 and is limited in movement. A connecting plate 25 is fixedly connected to one side of each two adjacent guide plates 20. A culture unit 17 is fixedly connected to the top of each two adjacent connecting plates 25. Limiting holes are opened on one side of each two adjacent guide rails 18. 19. When the guide plate 20 moves, the limiting ball 23 also moves. When it moves to the position of the limiting hole 19, the spring 22 rebounds because there is no force, and then engages with the limiting hole 19. Limiting grooves 21 are opened on one side of each of the two adjacent guide plates 20. Springs 22 are fixedly connected to one side of the inner wall of each of the two adjacent limiting grooves 21. Limiting balls 23 are fixedly connected to one side of each of the two adjacent springs 22. Fine pore grids 26 are fixedly connected to one side of each culture unit 17. Fine pore grids 26 facilitate nutrient interaction between the cell culture system and the culture medium. A conveying component is provided on one side of the outer ring of the culture solution container body 1.

[0035] Combination Figure 6 The delivery assembly includes an inlet pipe 5, which passes through and is fixedly connected to the culture solution container body 1. The inlet pipe 5 allows the cell hydrogel system to be delivered into the first transport pipe 11 and the second transport pipe 10 in the required quantity and density, and then discharged from the first outlet pipe 13. One end of the inlet pipe 5 passes through and is fixedly connected to the first transport pipe 11, and the other end of the first transport pipe 11 passes through and is provided with a rotary joint 12. When the support rod 8 is removed, the second transport pipe 10 needs to be separated from the first transport pipe 11. The right end of the inlet pipe 5 passes through and is fixedly connected to a second solenoid valve 29. The inner ring of the rotary joint 12 is provided with the second transport pipe 10, and the inner ring of the support rod 8 is provided with a conduit 9. The conduit allows the liquid to enter and then be discharged from the first outlet pipe 13. The outer ring of the conduit 9 passes through and is fixedly connected to the evenly distributed first outlet pipes 13. The other end of the first outlet pipe 13 is fixedly connected to the support rod 8, and the first outlet pipe 13 passes through and extends to one side of the support rod 8.

[0036] Working Principle: When using the culture device, first sterilize the device and connect an external peristaltic pump to the second solenoid valve 29 and the first solenoid valve 28. Then, rotate and remove the lid 2, place an appropriate amount of culture medium into the culture unit 17, start the peristaltic pump until circulation is complete and air is removed, then use the feed head of the peristaltic pump to deliver to the inlet pipe 5, and open the second solenoid valve 29 to deliver the cell hydrogel system into the first transport pipe 11 and the second transport pipe 10 according to the required quantity and density, and then discharge it from the first outlet pipe 13 into the culture unit 17 for loading. Then, rotate and seal the lid 2 to begin the culture process. The sides and bottom of the culture unit 17 are covered with fine-pore grids for easy exchange. When changing the medium, disconnect from the peristaltic pump, open the first solenoid valve 28 to discharge the waste liquid from the first solenoid valve 28, and drain the dry liquid. After cleaning, connect the peristaltic pump and use it to slowly add liquid through the inlet pipe 5. The peristaltic pump continuously circulates the culture. During use, the support rod 8 can be moved upwards to separate the square block 14 from the second support frame 15, allowing the support rod 8 to be pulled out of the culture solution container body 1. Simultaneously, the second transport pipe 10 separates from the rotary joint 12. Then, pull the first handle 24 to forcefully pull the limiting ball 23 out of the limiting hole 19, allowing the culture unit 17 to be separated and replaced. During installation, simply insert the guide plate 20 into the limiting hole 19 and push it forcefully. When the limiting ball 23 moves to the position of the limiting hole 19, the spring 22 rebounds, and the limiting ball 23 engages with the limiting hole 19, completing the installation of the culture unit 17. This facilitates replacement and improves the performance. Except for the rubber transport pipe, the rest of the device is made of transparent material for easy observation.

[0037] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0038] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A culture apparatus for three-dimensional cells, comprising a culture solution container body (1), characterized in that: A conical hopper (4) is fixedly connected to the bottom of the culture solution container body (1). A second support frame (15) is fixedly connected between the inner walls of the culture solution container body (1). A square block (14) is slidably connected through the top of the second support frame (15). A support rod (8) is fixedly connected to the top of the square block (14). A uniformly distributed connecting ring (16) is fixedly connected to the outer ring of the support rod (8). A uniformly distributed guide rail (18) is fixedly connected to the top of the connecting ring (16). A guide plate (20) is slidably connected between two adjacent guide rails (18). One side of two adjacent guide plates (20) Each of the two adjacent connecting plates (25) is fixedly connected to a connecting plate (25). A culture unit (17) is fixedly connected to the top of each of the two adjacent guide rails (18). A limit hole (19) is opened on one side of each of the two adjacent guide plates (20). A limit groove (21) is opened on one side of each of the two adjacent limit grooves (21). A spring (22) is fixedly connected to one side of each of the two adjacent springs (22). A limit ball (23) is fixedly connected to one side of each of the two adjacent springs (22). A fine-pore grid (26) is fixedly connected to one side of each culture unit (17). A conveying component is provided on one side of the outer ring of the culture solution container body (1).

2. The device for culturing three-dimensional cells according to claim 1, characterized in that: The delivery assembly includes an inlet pipe (5), which passes through and is fixedly connected to the culture solution container body (1). One end of the inlet pipe (5) passes through and is fixedly connected to a first transport pipe (11). The other end of the first transport pipe (11) passes through and is provided with a rotary joint (12). The right end of the inlet pipe (5) passes through and is fixedly connected to a second solenoid valve (29). The inner ring of the rotary joint (12) is provided with a second transport pipe (10). The inner ring of the support rod (8) passes through and is provided with a conduit (9). The outer ring of the conduit (9) passes through and is fixedly connected to a uniformly distributed first outlet pipe (13). The other end of the first outlet pipe (13) is fixedly connected to the support rod (8), and the first outlet pipe (13) passes through and extends to one side of the support rod (8).

3. The device for culturing three-dimensional cells according to claim 1, characterized in that: The bottom of the conical hopper (4) is connected to a second liquid outlet pipe (27), and the bottom of the second liquid outlet pipe (27) is connected to a first solenoid valve (28).

4. The device for culturing three-dimensional cells according to claim 1, characterized in that: A first handle (24) is fixedly connected to one side of the culture unit (17).

5. The device for culturing three-dimensional cells according to claim 1, characterized in that: The limiting hole (19) engages with the limiting ball (23).

6. The device for culturing three-dimensional cells according to claim 1, characterized in that: The outer ring of the culture solution container body (1) has two air inlet pipes (6) that are connected through and fixedly connected. The outer ring of the two air inlet pipes (6) is threaded with a filter screen (7). The bottom of the outer ring of the culture solution container body (1) is provided with a first support frame (3).

7. The device for culturing three-dimensional cells according to claim 1, characterized in that: The outer ring of the culture solution container body (1) is threaded with a cap (2), and a second handle (30) is fixedly connected to the top of the cap (2).

8. The device for culturing three-dimensional cells according to claim 1, characterized in that: The second transport pipe (10) is connected through and fixedly connected to one side of the outer ring of the support rod (8), and a conduit (9) is connected through and fixedly connected to one end of the second transport pipe (10).

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