Culture dish for immunofluorescent staining of oocyte / early embryo

Abstract

The utility model discloses a culture dish for immunofluorescent staining of oocytes / early embryos. The culture dish comprises a dish bottom and an upper cover matched with the dish bottom, a plurality of grooves for containing oocytes / early embryos are formed in the dish bottom and the upper cover; a plurality of independent areas are formed among the grooves in a line drawing manner in advance, so that the grooves are distributed on the dish bottom and the upper cover to group the dyeing dishes. The immunofluorescence staining dish disclosed by the utility model is small and practical, and the design and the use of the immunofluorescence staining dish avoid the tedious steps of grouping by using mark stroke lines on a conventional cell culture dish and using an immunohistochemical stroke range before / during the staining process; the preparation work and time before oocyte / early embryo immunofluorescence staining are greatly reduced, the possibility of liquid crosstalk and sample confusion among groups is avoided, the experiment efficiency is improved, and the experiment process is accelerated.

Description

Technical Field

[0001] This invention belongs to the field of biological instrument and equipment technology, and more specifically, relates to a culture dish for immunofluorescence staining of oocytes / early embryos. Background Technology

[0002] Immunofluorescence staining of oocytes / early embryos is typically performed on standard cell culture dishes, involving 2-4 groups: control group, treatment group 1, treatment group 2, and treatment group 3. Oocytes / early embryos are suspension cells and cannot adhere to the culture dish. Before immunofluorescence staining, demarcation lines are drawn on the standard cell culture dish using a marker to group the cells and label the groups. Then, multiple circles are drawn using an immunohistochemistry pen to mark the placement area for each step's solution (approximately 50 μl). Because the lines drawn by the immunohistochemistry pen are above the surface of the culture dish, they prevent the solution from spreading. The suspended oocytes / early embryo cells are then sequentially transferred into the solution for each step of the experiment.

[0003] In practice, an immunofluorescence staining experiment requires at least 3-4 culture dishes. If the number of groups increases or if staining experiments for multiple antibodies are performed simultaneously, the number of culture dishes used will be even greater. The preparatory work of drawing lines, marking group names, and drawing circles before the experiment increases exponentially, which greatly reduces experimental efficiency and increases the error rate. In addition, the height of the immunohistochemistry pen mark is limited, and the buffer solution often overflows outside the circle due to its own large extensibility or slight collisions during the experiment. This causes the liquids and samples of each group to mix and interfere with each other. The diffused liquid will evaporate faster and affect the concentration, ultimately interfering with the experimental results. Utility Model Content

[0004] To address the aforementioned issues, this invention provides a culture dish for immunofluorescence staining of oocytes / early embryos. By incorporating grouping and staining grooves during the fabrication of the culture dish, the preparation work and error rate before immunofluorescence staining of oocytes / early embryos are significantly reduced, and the possibility of liquid crosstalk between groups is further reduced or even eliminated.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] An immunofluorescence staining culture dish for oocytes / early embryos includes a dish bottom and a matching top cover; both the dish bottom and the top cover are provided with multiple grooves for holding oocytes / early embryos; the grooves are pre-marked with lines to form several independent areas, and the group names are marked with numbers, so that the grooves are arranged in a certain way on the dish bottom and the top cover to group the staining dishes.

[0007] In one embodiment of this utility model, the scribing line is at least one straight line along the diameter direction provided on the bottom of the dish and the top cover.

[0008] In one embodiment of this utility model, the number of lines is 2-4.

[0009] In one embodiment of this utility model, the scribing lines are two mutually perpendicular straight lines along the diameter direction on the bottom of the dish and the top cover, one half of which is a dashed line and marked with an arrow to indicate the direction.

[0010] In one embodiment of this utility model, the scribing line includes a straight line along the diameter direction on the bottom of the dish and the top cover, and a circle at the center position.

[0011] In one embodiment of this utility model, different partitions are marked with auxiliary markers numbered 1-4 to indicate grouping.

[0012] For example, the grooves are pre-marked with lines to form four independent areas, and the group names are marked with numbers 1-4.

[0013] In one embodiment of this utility model, the plane diameter of the groove is 0.7-1.2 cm and the depth is 0.2-0.5 cm.

[0014] In one embodiment of this utility model, the plane diameter of the groove is 1.0 cm and the depth is 0.35 cm.

[0015] In one embodiment of this utility model, the volume of the groove is 100-150 μL.

[0016] In one embodiment of this utility model, the diameter of the culture dish is 5-7 cm.

[0017] In one embodiment of this utility model, 10-16 grooves are provided on both the bottom of the dish and the top cover.

[0018] In one embodiment of this utility model, 12 grooves are provided on both the bottom of the dish and the top cover.

[0019] In one embodiment of this utility model, the bottom surface of the groove has an arc-shaped structure.

[0020] The beneficial effects of this utility model are:

[0021] While existing conventional cell culture dishes can be pre-marked with lines to group cells and define liquid placement areas for immunofluorescence staining experiments, this increases pre-experimental preparation time and easily leads to mixing of liquids and samples between groups, affecting experimental results. The immunofluorescence staining dish provided by this invention is compact and practical. Its design and use avoid the cumbersome steps of marking groups with markers, labeling group names, and using immunohistochemical markers to define areas on conventional cell culture dishes before / during the staining process. By pre-setting grouping and staining grooves on the culture dish itself, it not only significantly reduces the preparation work and time before immunofluorescence staining of oocytes / early embryos but also avoids possible liquid crosstalk and sample mixing between groups, improving experimental efficiency and accelerating the experimental process. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of the culture dish for immunofluorescence staining according to this utility model;

[0023] Figure 2 This is a side cross-sectional view of the culture dish used for immunofluorescence staining according to this invention;

[0024] Figure 3 This is a top view of the culture dish for immunofluorescence staining according to this invention;

[0025] Figure 4 This is a schematic diagram of the grouping of culture dishes used for immunofluorescence staining according to this invention. Figure 1 ;

[0026] Figure 5 This is a schematic diagram of the grouping of culture dishes used for immunofluorescence staining according to this invention. Figure 2 ;

[0027] Figure 6 This is a schematic diagram of the grouping of culture dishes used for immunofluorescence staining according to this invention. Figure 3 . Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be emphasized that the specific embodiments described herein are only for better illustrating the present utility model and represent some, not all, embodiments, and therefore are not intended to limit the present utility model. Furthermore, the technical features involved in the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0029] like Figures 1 to 6As shown, this utility model provides a culture dish for immunofluorescence staining of oocytes / early embryos, including a dish bottom 10 and a matching top cover 20; both the dish bottom 10 and the top cover 20 are provided with a plurality of grooves 30 for holding oocytes / early embryos, the grooves 30 are pre-divided into several independent areas by drawing lines and the group names are marked with numbers, so that the grooves form a certain arrangement on the dish bottom 10 and the top cover 20 to group the staining dishes.

[0030] This invention allows for the proper and convenient grouping of culture dishes in advance by designing and planning the number and arrangement of staining grooves on the culture dish.

[0031] Preferably, the scribing line is at least one straight line along the diameter direction on the bottom 10 and the top cover 20 of the dish. For example, the number of scribing lines is 2-4.

[0032] In one specific embodiment, the scribing lines are two mutually perpendicular straight lines along the diameter direction on the bottom 10 and the top cover 20 of the dish, one half of which is a dashed line and marked with an arrow to indicate the direction.

[0033] In another specific embodiment, such as Figure 3 As shown, the scribing lines include a straight line along the diameter direction on the bottom 10 and the top cover 20 of the dish, and a circle located at the center.

[0034] Preferably, the diameter of the culture dish can be 5 to 7 cm.

[0035] Preferably, both the bottom 10 and the top cover 20 are provided with 10-16 grooves 30. For example, both the bottom 10 and the top cover 20 are provided with 12 grooves 30. Preferably, the bottom surface of the grooves 30 is arc-shaped.

[0036] Preferably, the planar diameter of the groove 30 is 0.7–1.2 cm, and the depth is 0.2–0.5 cm. For example, the planar diameter of the groove 30 is 1.0 cm, and the depth is 0.35 cm.

[0037] Example 1

[0038] The following steps were taken to streak oocytes / early embryos using immunofluorescence staining on a culture dish:

[0039] An immunofluorescence staining culture dish with a diameter of 6 cm was used. Both the bottom and top of the dish were made of plastic. Each dish had 12 staining grooves, each 1 cm in diameter and 0.35 cm deep, with an arc-shaped bottom. The oocytes were 75 μm in diameter, and the groove diameter was much larger than the oocyte diameter. The specific distribution and pattern are shown below. Figure 1 and Figure 2 As shown.

[0040] The bottom of the culture dishes used for immunofluorescence staining and their corresponding lids are marked with straight lines, circles, and numbers 1-4 to indicate groupings. Dashed lines and arrows are used to further indicate the orientation of the circular dishes. Each culture dish has 12 recesses. If the experimental grouping is a control group and treatment group 1, it is divided into two groups (1 and 2), each using 6 recesses. Figure 4 If the experimental grouping is control group, treatment group 1, and treatment group 2, then it is divided into three groups: left, right, and middle (1, 2, 3), each using 4 grooves. Figure 5 If the experimental grouping is control group, treatment group 1, treatment group 2, and treatment group 3, then it is divided into four groups: upper left, upper right, lower left, and lower right (1, 2, 3, 4), each using 3 grooves. Figure 6 There is no need to draw lines for grouping, label groups, or use immunohistochemistry lines, which saves experimental preparation time and reduces the error rate.

[0041] Each fluorescent staining droplet typically requires 50 μL, and the recessed design can hold 150 μL of liquid, which is sufficient to avoid liquid spillage and mixing problems caused by liquid spreadability and handling collisions during experiments. At the same time, the top cover can also be grouped and recessed in a similar way to the bottom of the dish, and can be used as the bottom of the dish, with the original bottom simply placed on top.

[0042] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A culture dish for immunofluorescence staining of oocytes / early embryos, characterized in that, Includes a dish bottom (10) and a matching top cover (20); both the dish bottom (10) and the top cover (20) are provided with multiple grooves (30) for holding oocytes / early embryos; the grooves (30) are pre-marked with lines to form several independent areas, and the group names are marked with numbers, so that the grooves are arranged in a certain way on the dish bottom (10) and the top cover (20) to group the staining dishes; The groove (30) has a plane diameter of 0.7~1.2 cm and a depth of 0.2~0.5 cm.

2. The culture dish for immunofluorescence staining of oocytes / early embryos according to claim 1, characterized in that, The line is at least one straight line along the diameter direction on the bottom (10) and top cover (20) of the dish.

3. The culture dish for immunofluorescence staining of oocytes / early embryos according to claim 2, characterized in that, The number of lines drawn is 2-4.

4. The culture dish for immunofluorescence staining of oocytes / early embryos according to claim 2, characterized in that, The lines are two mutually perpendicular straight lines set on the bottom (10) and top cover (20) of the dish along the diameter direction. One half of the straight line is a dashed line and is marked with an arrow to indicate the direction.

5. The culture dish for immunofluorescence staining of oocytes / early embryos according to claim 1, characterized in that, The lines drawn include straight lines along the diameter direction on the bottom (10) and top cover (20) of the dish, and a circle at the center.

6. The culture dish for immunofluorescence staining of oocytes / early embryos according to claim 1, characterized in that, The diameter of the culture dish is 5-7 cm.

7. The culture dish for immunofluorescence staining of oocytes / early embryos according to claim 1, characterized in that, The bottom (10) and top cover (20) of the dish are each provided with 10-16 grooves (30).

8. The culture dish for immunofluorescence staining of oocytes / early embryos according to claim 1, characterized in that, The bottom (10) and top cover (20) of the dish are each provided with 12 grooves (30).

9. The culture dish for immunofluorescence staining of oocytes / early embryos according to claim 1, characterized in that, The bottom surface of the groove (30) is an arc-shaped structure.

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