Centrifugal tube capable of rapidly purifying protein

Abstract

The utility model discloses a centrifuge tube capable of quickly purifying protein, which relates to the technical field of centrifuge tubes, and comprises an upper tube body, the lower end of the upper tube body is in threaded connection with a lower tube body, a filter mechanism is arranged at the joint of the upper tube body and the lower tube body, a separation tube is arranged in the upper tube body, and a filter mechanism is arranged in the separation tube. Two symmetrical liquid through holes are formed in the lower end of the separation pipe, sealing parts are arranged at the liquid through holes, and a fixing module is arranged at the upper end of the separation pipe; large protein is intercepted in the lower pipe body through the filtering mechanism, liquid supernatant and small impurities can enter the upper pipe body through the filtering mechanism, the liquid supernatant and the small impurities are sealed in the separation pipe through the sealing component on the separation pipe, and therefore the liquid supernatant and the large protein are separated, subsequent further operation is facilitated, and the separation efficiency is improved. The separation effect is effectively improved, and the working efficiency is improved; and the separation pipe can be quickly disassembled and assembled through the fixing module, so that supernate is conveniently taken out.

Description

TECHNICAL FIELD

[0001] The utility model relates to centrifugal tube technical field, and specifically is a centrifugal tube of fast protein purification. BACKGROUND

[0002] Proteins in tissues or cells are generally in the form of a complex mixture, and each type of cell contains thousands of different proteins. The overall goal of protein separation and purification is to increase the purity of the product, and the requirement for purification is to separate the desired protein from the impurities with reasonable efficiency, speed, yield and purity. Protein separation requires the use of centrifugal tubes. Biological sample suspension is rotated at high speed in the centrifugal tube. Due to the action of the centrifugal force, the suspended small particles (such as organelles, biological macromolecular precipitates, etc.) settle at a certain speed, thereby separating from the solution.

[0003] The existing centrifugal tube usually needs manual pipetting operation of the supernatant separated after centrifugation after purification, and then pouring the lower layer of macromolecular protein. However, manual separation of the supernatant is prone to contain impurities, which is not convenient for subsequent further operation, has poor separation effect, and has low work efficiency.

[0004] Therefore, the present application provides a centrifugal tube for fast protein purification, which can eliminate the drawbacks of the prior art. SUMMARY

[0005] The utility model discloses a centrifugal tube for fast protein purification, which can solve the problem of manual separation of the supernatant, which is prone to contain impurities, is not convenient for subsequent further operation, has poor separation effect, and has low work efficiency.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:

[0007] A centrifugal tube for fast protein purification includes an upper tube body, a lower tube body connected to the lower end of the upper tube body by screwing, a filter mechanism provided at the connection between the upper tube body and the lower tube body, a separation tube provided inside the upper tube body, two symmetrical liquid passage holes provided at the lower end of the separation tube, a sealing component provided at the liquid passage holes, and a fixing module provided at the upper end of the separation tube.

[0008] Based on the above technical scheme, the utility model also provides the following optional technical schemes:

[0009] In an optional scheme, the filter mechanism includes a fixed ring, a separation module fixedly connected inside the fixed ring, a fixed frame provided at the upper end of the separation module, the fixed frame fixedly connected to the inner wall of the fixed ring, and a fixed block fixedly connected to the upper end of the fixed frame.

[0010] In an alternative: the sealing component comprises a rotating block, the rotating block is rotationally connected at the lower end of the separation tube, the outer end of the rotating block is fixedly connected with two symmetrically arranged baffles, the baffles are located corresponding to the liquid passage hole, and the lower end of the rotating block is provided with a clamping groove corresponding to the fixed block.

[0011] In an alternative: the fixed module comprises four limiting blocks, the four limiting blocks are fixedly connected at the upper end of the separation tube, and the upper end of the upper tube body is provided with a limiting groove corresponding to the limiting block.

[0012] In an alternative: the outer side wall surface of the separation tube is provided with an annular protruding block, the side of the protruding block away from the separation tube abuts against the inner wall of the upper tube body, and the protruding block is made of rubber.

[0013] In an alternative: the upper end of the separation tube is fixedly connected with a sealing cover.

[0014] In an alternative: the upper end surface of the baffle completely covers the liquid passage hole.

[0015] In an alternative: the four limiting blocks are uniformly arranged on the separation tube.

[0016] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0017] The utility model discloses a filtering mechanism, and larger proteins are intercepted in the lower tube body, and supernatant and smaller impurities can pass through the filtering mechanism and enter the upper tube body, the sealing component on the separation tube seals the supernatant and smaller impurities in the separation tube, so that the supernatant and larger proteins are separated, subsequent further operation is facilitated, the separation effect is effectively improved, and the working efficiency is improved. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is the structure schematic drawing of the utility model.

[0019] Figure 2 It is the sectional view of the utility model.

[0020] Figure 3 It is the structure schematic drawing of A of the utility model.

[0021] Figure 4 It is the structure schematic drawing of B of the utility model.

[0022] Figure 5 It is the structure schematic drawing of the separation tube of the utility model.

[0023] The reference signs are annotated as follows: 100, upper tube body, 200, lower tube body, 300, separation tube, 301, liquid passage hole, 401, fixed ring, 402, separation die, 403, fixed frame, 404, fixed block, 501, rotating block, 502, baffle, 503, clamping groove, 601, limiting block, 602, limiting groove, 700, protruding block, 800, sealing cover. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical scheme and advantages of the utility model more clearly, the utility model is further described in detail below in combination with the drawings and examples.

[0025] In one embodiment, as shown in Figures 1-5 A centrifuge tube capable of rapidly purifying protein, comprising an upper tube body 100, a lower end of the upper tube body 100 being threadedly connected to a lower tube body 200, a filter mechanism being arranged at a connection between the upper tube body 100 and the lower tube body 200, an inside of the upper tube body 100 being provided with a separation tube 300, a lower end of the separation tube 300 being provided with two symmetrical liquid passage holes 301, the liquid passage holes 301 being provided with sealing components, and an upper end of the separation tube 300 being provided with a fixed module. When the device is used, a liquid to be purified is first placed in the lower tube body 200, the upper tube body 100 is rotated by means of thread connection, the lower end of the upper tube body 100 is fixedly connected to the upper end of the lower tube body 200, the separation tube 300 is fixedly connected to the inside of the upper tube body 100 by means of the fixed module, and the separation tube 300 is placed in a centrifuge for centrifugation. Larger-mass proteins are blocked in the lower tube body 200 by means of the filter mechanism, and smaller-mass impurities enter the inside of the separation tube 300 in the upper tube body 100 by means of the filter mechanism. The separation tube 300 is taken out, the liquid passage holes 301 at the lower end of the separation tube 300 are automatically closed by the sealing components, and the protein is rapidly separated and purified.

[0026] In the embodiment, as shown in Figure 4 The filter mechanism comprises a fixed ring 401, the inside of the fixed ring 401 being fixedly connected to a separation die 402, an upper end of the separation die 402 being provided with a fixed frame 403, the fixed frame 403 being fixedly connected to the inner wall of the fixed ring 401, an upper end of the fixed frame 403 being fixedly connected to a fixed block 404, and the separation die 402 being fixedly connected to the connection between the lower end of the upper tube body 100 and the lower tube body 200 by means of the fixed ring 401. Different-mass proteins are separated and purified by means of the separation die 402, larger-mass proteins are blocked at the lower end of the separation die 402, smaller-mass proteins pass through the separation die 402 and enter the separation tube 300 through the liquid passage holes 301, the protein is rapidly separated and purified, and the device can be used for purifying different types of proteins by replacing the separation die 402 with different pore diameters, so that the protein purification is realized.

[0027] In one embodiment, as shown in Figure 4 and Figure 5 , the sealing component includes a rotating block 501, which is rotatably connected to the lower end of the separation tube 300, the outer end of the rotating block 501 is fixedly connected to two symmetrically arranged baffles 502, the baffles 502 are positioned corresponding to the liquid passage hole 301, the lower end of the rotating block 501 is provided with a clamping groove 503 corresponding to the fixed block 404, when the separation tube 300 is inserted into the upper tube body 100, the clamping groove 503 and the fixed block 404 cooperate with each other, the upper tube body 100 is rotated to drive the fixed block 404 to rotate, the fixed block 404 drives the rotating block 501 to rotate, and the rotating block 501 drives the two side fixedly connected baffles 502 to rotate, thereby opening the liquid passage hole 301, so that the liquid passing through the separation mold 402 can enter the separation tube 300 through the liquid passage hole 301, when the separation is completed, the upper tube body 100 is reversely rotated to drive the rotating block 501 to rotate, so that the baffle 502 resets to seal the liquid passage hole 301, and the liquid is sealed in the separation tube 300, thereby rapidly separating and purifying the protein.

[0028] In one embodiment, as shown in Figure 1 and Figure 3 , the fixed module includes four limiting blocks 601, which are fixedly connected to the upper end of the separation tube 300, the upper end of the upper tube body 100 is provided with a limiting groove 602 corresponding to the limiting block 601, when the separation tube 300 is inserted into the upper tube body 100, the limiting block 601 is clamped into the limiting groove 602, and as the separation tube 300 rotates, the limiting block 601 and the limiting groove 602 cooperate to fix the separation tube 300, when the separation tube 300 needs to be taken out, it only needs to be reversely rotated to take out.

[0029] In one embodiment, as shown in Figure 2 , the outer side wall surface of the separation tube 300 is provided with an annular protruding block 700, the side of the protruding block 700 away from the separation tube 300 abuts against the inner wall of the upper tube body 100, the protruding block 700 is rubber, the annular rubber protruding block 700 can seal between the separation tube 300 and the upper tube body 100, prevent leakage, and can increase the stability of the separation tube 300, prevent the separation tube 300 from shaking inside the upper tube body 100 during separation, and affect the separation effect.

[0030] In one embodiment, as shown in Figure 2 , the upper end of the separation tube 300 is fixedly connected to a sealing cover 800, which seals the upper end of the separation tube 300 to prevent liquid leakage during separation.

[0031] In one embodiment, as shown in Figure 5 The upper end surface of the baffle 502 completely covers the liquid passage hole 301, so as to separate different quality proteins and realize protein purification.

[0032] In one embodiment, as shown in Figure 1 The four limiting blocks 601 are evenly arranged on the separation tube 300, so as to realize effective use of space and increase the stability of the separation tube 300.

[0033] The above embodiment discloses a centrifugal tube capable of rapidly purifying proteins. When the device is used, the liquid to be purified is first placed in the lower tube body 200, the upper tube body 100 is rotated by screw connection, the lower end of the upper tube body 100 is fixedly connected with the upper end of the lower tube body 200, the separation tube 300 is inserted into the upper tube body 100, the fixing block 404 is clamped into the clamping groove 503, the limiting block 601 is clamped into the limiting groove 602, the upper tube body 100 is rotated, the fixing block 404 is rotated, the fixing block 404 drives the rotating block 501 to rotate, the rotating block 501 drives the baffle 502 fixedly connected on both sides to rotate, so as to open the liquid passage hole 301, the limiting block 601 and the limiting groove 602 cooperate with each other to fix the separation tube 300, the sealing cover 800 seals the upper end of the separation tube 300, so as to prevent leakage, the separation tube 300 and the upper tube body 100 can be sealed, the stability of the separation tube 300 can be increased, and shaking during separation is prevented, so as to affect the separation effect, the device is placed in a centrifuge for centrifugation, the separation module 402 is fixedly connected to the lower end of the upper tube body 100 and the connection between the lower tube body 200 by the fixing ring 401, different quality proteins are separated and purified by the separation module 402, the larger quality proteins are blocked at the lower end of the separation module 402, and the smaller quality proteins pass through the separation module 402 and enter the separation tube 300 through the liquid passage hole 301, so as to rapidly separate and purify proteins. The device can also replace the separation module 402 with different pore sizes according to different sizes of proteins required, can be suitable for purification of different types of proteins, and realizes protein purification.

[0034] The above only describes specific embodiments of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art can easily think of changes or replacements within the technical range disclosed in the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A centrifuge tube that can rapidly purify a protein, comprising: The utility model provides an upper pipe body (100), its characterized in be that the lower end of the upper pipe body (100) is screwed down pipe body (200), the upper pipe body (100) and the lower pipe body (200) junctions are equipped with filter mechanism, the inside of the upper pipe body (100) is equipped with separation pipe (300), the lower end of the separation pipe (300) is equipped with two symmetry liquid passage (301), and the liquid passage (301) is equipped with sealing part, and the upper end of the separation pipe (300) is equipped with fixed module.

2. The centrifuge tube capable of rapidly purifying protein according to claim 1, characterized in that, The filter mechanism includes a fixed ring (401), the inside of the fixed ring (401) is fixedly connected with a separation die (402), the upper end of the separation die (402) is provided with a fixed frame (403), the fixed frame (403) is fixedly connected to the inner wall of the fixed ring (401), and the upper end of the fixed frame (403) is fixedly connected with a fixed block (404).

3. The centrifuge tube of claim 1, wherein, The sealing part includes a rotating block (501), the rotating block (501) is rotatably connected to the lower end of the separation pipe (300), the outer end of the rotating block (501) is fixedly connected with two symmetrically arranged baffles (502), the baffles (502) are located corresponding to the liquid passage (301), and the lower end of the rotating block (501) is provided with a clamping groove (503) corresponding to the fixed block (404).

4. The centrifuge tube of claim 1, wherein, The fixed module includes four limiting blocks (601), the four limiting blocks (601) are fixedly connected to the upper end of the separation pipe (300), and the upper end of the upper pipe body (100) is provided with a limiting groove (602) corresponding to the limiting block (601).

5. The centrifuge tube of claim 1, wherein, The outer side wall surface of the separation pipe (300) is provided with an annular protruding block (700), the side, away from the separation pipe (300), of the protruding block (700) abuts against the inner wall of the upper pipe body (100), and the protruding block (700) is made of rubber.

6. The centrifuge tube of claim 1, wherein, The upper end of the separation pipe (300) is fixedly connected with a sealing cover (800).

7. The centrifuge tube of claim 3, wherein, The upper end surface of the baffle (502) completely covers the liquid passage (301).

8. The centrifuge tube of claim 4, wherein, The four limiting blocks (601) are evenly arranged on the separation pipe (300).

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