Gene synthesis device
The sealing plate design and drive assembly with rotation axis positioning solve the problems of unstable sealing solution and incomplete drainage in gene synthesis devices, achieving sealing of the synthesis column and no contamination of reagents, thus improving synthesis efficiency and quality.
Patent Information
- Application Number
- CN202423127576.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-18
- Publication Date
- 2025-11-07
- Estimated Expiration
- 2034-12-18
AI Technical Summary
In existing gene synthesis technologies, problems such as unstable sealing solution and incomplete drainage affect synthesis efficiency and quality.
The sealing plate design adopts a rotating shaft positioning system. The rotating shaft serves as the positioning reference to prevent the sealing plate from tilting. Combined with the drive assembly, it achieves the sealing fit and separation between the sealing plate and the synthesis column, ensuring a sealed connection between the synthesis column and the collection box, and using gravity to complete the drainage.
This solved the problems of unstable sealing solution and incomplete drainage, ensuring the sealing of the synthesis column, avoiding reagent contamination, and improving synthesis efficiency and quality.
Smart Images
Figure CN223517507U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the technical field of genetic engineering, and particularly relates to a gene synthesis device. BACKGROUND
[0002] Gene synthesis is a technique for synthesizing genes by artificial method, and is one of means for obtaining genes, and a plurality of reagents need to be proportioned in each round of synthesis process, each round is new reagent, and the liquid volume of the reagent needs to be a stable value, and a plurality of reagent liquids cannot be doped. At present, the artificial synthesis method has experienced the development process from single channel to multiple channels. From the initial single channel controlled by the electromagnetic valve to the current multiple channel synthesis method, the existing technology realizes the plugging and drainage of multiple channels through the up-down reciprocating motion of the piston. In the whole reciprocating motion process of plugging and draining, the plugging and draining are often unstable and incomplete, thereby affecting the efficiency and quality of synthesis. UTILIZY MODEL CONTENT
[0003] The utility model provides a gene synthesis device, aims at solving the technical problem proposed in the above background art.
[0004] To achieve the above object, the utility model adopts the technical scheme of providing a gene synthesis device, comprising:
[0005] The synthesis assembly comprises a synthesis seat and a plurality of synthesis columns, the plurality of synthesis columns are arranged on the synthesis seat, the bottom is provided with an opening and is on the same plane, and the bottom of the synthesis column is flush with the bottom of the synthesis seat or protrudes from the bottom of the synthesis seat.
[0006] The liquid collecting box is arranged on the lower side of the synthesis seat and is connected and sealed with the synthesis seat.
[0007] The sealing plate is arranged in the liquid collecting box and is provided with a rotating shaft at one end, the rotating shaft is rotationally connected with the liquid collecting box, the sealing plate is adapted to rotate around the rotating shaft and is sealed and combined with the bottom of the synthesis column or separated from the bottom of the synthesis column.
[0008] The driving assembly is connected with the sealing plate at the power output end and is used to drive the sealing plate to rotate around the rotating shaft.
[0009] In a possible implementation manner of the gene synthesis device provided by the utility model, the synthesis seat comprises a synthesis base plate and a heating base plate, the heating base plate is arranged on the upper side of the synthesis base plate and is connected with the synthesis base plate, and the synthesis column penetrates through the heating base plate and the synthesis base plate and is connected with the heating base plate and the synthesis base plate.
[0010] In a possible implementation manner of the gene synthesis device, the synthesis seat further comprises a heat insulation pad, the heat insulation pad is arranged on the upper side of the heating base plate and is attached to the heating base plate, and the synthesis column passes through the heating base plate.
[0011] In a possible implementation manner of the gene synthesis device, the driving assembly comprises:
[0012] A rocker arm is arranged on the lower side of the end of the sealing plate away from the rotating shaft and is rotationally connected to the liquid collecting box at one end.
[0013] A pushing block is arranged on the lower side of the sealing plate and is connected to the side of the rocker arm facing the rotating shaft, and is adapted to rotate the sealing plate with the rocker arm to push the sealing plate, so that the sealing plate is in sealing attachment with the bottom of the synthesis column or is separated from the bottom of the synthesis column.
[0014] A driving mechanism is connected to the rocker arm at the power output end and is used to drive the rocker arm to rotate.
[0015] In a possible implementation manner of the gene synthesis device, the end of the pushing block away from the rocker arm is in an arc structure.
[0016] In a possible implementation manner of the gene synthesis device, the end of the rocker arm away from the pushing block is provided with a sliding groove, the sliding groove extends along the length direction of the rocker arm, and the driving mechanism comprises:
[0017] A motor assembly is fixedly arranged; and
[0018] A crank is connected to the power output end of the motor assembly at one end and is arranged in the sliding groove at the other end, and has the freedom of rotating in the sliding groove and sliding along the length direction of the sliding groove.
[0019] In a possible implementation manner of the gene synthesis device, a base is further arranged on the lower side of the liquid collecting box and is connected to the liquid collecting box, and the motor assembly is connected to the base.
[0020] The gene synthesis device has the following beneficial effects: compared with the prior art, the gene synthesis device provided by the utility model takes the rotating shaft as a positioning reference during the rotation of the sealing plate, avoids the left-right inclination of the sealing plate, ensures the parallelism of the sealing plate and the synthesis seat, solves the problem of liquid leakage of the synthesis column, and enables the liquid on the sealing plate to naturally slide into the liquid collecting box under the action of gravity during the liquid discharge operation, so that the reagent in the synthesis column is prevented from being contaminated. BRIEF DESCRIPTION OF DRAWINGS
[0021] Figure 1The utility model provides a three -dimensional structure schematic diagram of gene synthesis device provides for the embodiment of the utility model,
[0022] Figure 2 The utility model provides a main view structure schematic diagram of synthesis module in gene synthesis device provides for the embodiment of the utility model,
[0023] Figure 3 The utility model provides a main view structure schematic diagram of gene synthesis device removes the liquid collecting box and is in the main view structure schematic diagram of liquid -tight state,
[0024] Figure 4 The utility model provides a main view structure schematic diagram of gene synthesis device removes the liquid collecting box and is in the main view structure schematic diagram of liquid -tight state,
[0025] Mark explanation:
[0026] 1, synthesis module, 2, liquid collecting box, 3, drive assembly, 4, base,
[0027] 101, synthesis column, 102, liquid separation pad, 103, heating base plate, 104, synthesis base plate,
[0028] 301, motor assembly, 302, crank, 303, rocker arm, 304, turnover shaft,
[0029] 305, push block, 306, rotating shaft, 307, sealing plate. Specific embodiments
[0030] In order to make the technical problem, technical scheme and beneficial effect that the utility model wants to solve more clearly understood, the following combining with the drawing and embodiment, the utility model is further detailedly described.It should be understood that the specific embodiments described here are only used to explain the utility model, and are not used to limit the utility model.
[0031] The technical scheme in the embodiment of the application will be described clearly and completely in the embodiment of the application, obviously, the described embodiment is only a part of the embodiment of the application, not all embodiments. The description of the following at least one exemplary embodiment is actually only illustrative, and is by no means any limitation on the application and its application or use. Based on the embodiment in the application, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of the application.
[0032] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and / or "comprising," when used in this specification, specify the presence of stated features, steps, operations, elements, components, and / or groups thereof, but do not preclude the presence or addition of one or more other features, steps, operations, elements, components, and / or groups thereof.
[0033] The relative arrangement of components and steps, numerical expressions, and numerical values set forth in the examples are not intended to limit the scope of the application unless otherwise specifically stated. It is to be understood that the drawings are not necessarily to scale of the actual proportions used in the fabrication, assembly, and operation of the example embodiments. Techniques, methods, and apparatus known to those of ordinary skill can not be discussed in detail herein, but should be considered as part of the description unless otherwise stated. In all examples shown and discussed herein, any specific values are to be interpreted as merely exemplary and not limiting. Thus, other examples of example embodiments can have different values. It is noted that like references and designations can indicate like items throughout the several views of the drawings, and thus, once an item is defined in one view, it is not necessary to discuss it further in subsequent views unless explicitly stated.
[0034] In the description of the present application, it is to be understood that the orientation or positional relationships indicated by orientation words such as "front, back, upper, lower, left, right", "horizontal, vertical, perpendicular, horizontal" and "top, bottom" are generally based on the orientation or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description. Without the opposite indication, these orientation words do not indicate and imply that the indicated device or element must have a particular orientation or be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the scope of protection of the present application. The orientation words "inner, outer" refer to the inner and outer relative to the contour of each component.
[0035] For purposes of the description hereinafter, spatial relative terms, such as "above", "below", "upper", "lower", and the like, can be used to describe the relative position of one element or feature to another element or feature as illustrated in the figures. It will be understood that the spatial relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientations depicted in the figures. For example, if the device in the figures is turned over, elements described as "above" or "up" other elements or features would then be oriented "below" or "down" the other elements or features. Thus, the exemplary term "above" can encompass both an orientation of above and below. The device can be otherwise oriented (e.g., rotated 90 degrees or at other orientations) and the spatial relative terms used herein interpreted accordingly.
[0036] In addition, it should be noted that the use of "first", "second", and the like words of distinction do not connote any meaning of importance, but are used only to distinguish one element from another, and are used subject to change only where the context clearly manifests that meaning.
[0037] Please refer to Figures 1 to 4 The gene synthesis device comprises a synthesis assembly, a liquid collecting box 2, a sealing plate 307 and a driving assembly 3. The synthesis assembly comprises a synthesis seat and a plurality of synthesis columns 101. The plurality of synthesis columns 101 are arranged on the synthesis seat, are provided with openings at the bottom and are located on the same plane, and the bottom of the synthesis column 101 is flush with the bottom of the synthesis seat or protrudes from the bottom of the synthesis seat. The liquid collecting box 2 is arranged on the lower side of the synthesis seat, is connected with the synthesis seat and is in sealing cooperation. The sealing plate 307 is arranged in the liquid collecting box 2, is provided with a rotating shaft 306 at one end, is in rotating cooperation with the liquid collecting box 2, is adapted to rotate around the rotating shaft 306, is in sealing and close cooperation with the bottom of the synthesis column 101 or is separated from the bottom of the synthesis column 101, and the driving assembly 3 is connected with the sealing plate 307 and is used to drive the sealing plate 307 to rotate around the rotating shaft 306.
[0038] It should be noted that the plurality of synthesis columns 101 are arranged on the synthesis seat in an array along the width and length directions of the synthesis seat. The synthesis column 101 is tubular and penetrates up and down. During synthesis, the bottom is blocked by the sealing plate 307.
[0039] The gene synthesis device has the advantages that, compared with the prior art, the gene synthesis device provided by the utility model takes the rotating shaft 306 as a positioning reference during the rotating process of the sealing plate 307, avoids the left and right inclination of the sealing plate 307, guarantees the parallelism of the sealing plate 307 and the synthesis seat, and solves the liquid leakage of the synthesis column 101; when the liquid discharging action is performed, the sealing plate 307 is turned downward and separated from the bottom of the synthesis column 101, the liquid on the sealing plate 307 naturally slides into the liquid collecting box 2 under the action of gravity, the liquid discharging is completed, and the reagent in the synthesis column 101 is prevented from being polluted.
[0040] As shown in Figure 1 and Figure 2 in a specific embodiment of the gene synthesis device provided by the utility model embodiment, the synthesis seat comprises a synthesis substrate 104 and a heating substrate 103, the heating substrate 103 is arranged on the upper side of the synthesis substrate 104 and is connected with the synthesis substrate 104; the synthesis column 101 passes through the heating substrate 103 and the synthesis substrate 104 and is connected with the heating substrate 103 and the synthesis substrate 104.
[0041] Further, as shown in Figure 1 and Figure 2 in a specific embodiment of the gene synthesis device provided by the utility model embodiment, the synthesis seat further comprises a heat insulation pad, the heat insulation pad is arranged on the upper side of the heating substrate 103 and is attached to the heating substrate 103, and the synthesis column 101 passes through the heating substrate 103.
[0042] It should be noted that the synthesis column 101 is mounted on the heating substrate 103 and is separated by the liquid separation pad 102, the liquid separation pad 102 protects the heat of the heating substrate 103 from being excessively lost, thereby guaranteeing the heating speed of the synthesis column 101. The synthesis module 1 is sealingly connected with the liquid collecting box 2 through the synthesis substrate 104.
[0043] As shown in Figure 3 and Figure 4 in a specific embodiment of the gene synthesis device provided by the utility model embodiment, the driving assembly 3 comprises a rocker arm 303, a pushing block 305 and a driving mechanism, the rocker arm 303 is arranged on the lower side of one end of the sealing plate 307 away from the rotating shaft 306 and is rotationally matched with the liquid collecting box 2 at one end; the pushing block 305 is arranged on the lower side of the sealing plate 307 and is connected with one side of the rocker arm 303 facing the rotating shaft 306, is adapted to rotate the pushing block 305 to push the sealing plate 307, so that the sealing plate 307 is sealingly attached to or separated from the bottom of the synthesis column 101; the driving mechanism is connected with the rocker arm 303 at the power output end and is used to drive the rocker arm 303 to rotate.
[0044] Specifically, the rocker arm 303 is provided with a turnover shaft 304 at one end, the turnover shaft 304 is rotationally matched with at least one of the rocker arm 303 or the liquid collecting box 2, so that the rocker arm 303 is rotationally arranged on the liquid collecting box 2.
[0045] Further, as shown in a specific embodiment of the gene synthesis device provided in the embodiment of the utility model, Figure 3 and Figure 4 as shown, in a specific embodiment of the gene synthesis device provided in the embodiment of the utility model, the one end of the push block 305 away from the rocker arm 303 is arc-shaped, which reduces the contact stress on the one hand, and makes the process of the push plate 307 more stable on the other hand.
[0046] Specifically, as shown in a specific embodiment of the gene synthesis device provided in the embodiment of the utility model, Figure 3 and Figure 4 as shown, in a specific embodiment of the gene synthesis device provided in the embodiment of the utility model, the one end of the rocker arm 303 away from the push block 305 is provided with a sliding groove, the sliding groove extends along the length direction of the rocker arm 303, the driving mechanism includes a motor assembly 301 and a crank 302, and the motor assembly 301 is fixedly arranged; the crank 302 is connected with the power output end of the motor assembly 301 at one end, and is arranged in the sliding groove at the other end, and has the freedom of rotation in the sliding groove and sliding along the length direction of the sliding groove.
[0047] The plate 307 is positioned and connected with the liquid collecting box 2 through the rotating shaft 306; the motor assembly 301 drives the crank 302 to rotate, the crank 302 drives the rocker arm 303 to swing left and right, the rocker arm 303 forms a lever mechanism with the push block 305 through the turnover shaft 304, so that the push block 305 rotates around the turnover shaft 304 to drive the plate 307 to rotate around the rotating shaft 306 by a fixed angle to seal and fit with the synthesis substrate 104 or separate from the synthesis substrate 104.
[0048] It should be noted that the motor assembly 301 is a prior art device, which can be a stepper motor, a servo motor or a combination of the same and a speed reducer.
[0049] As shown in a specific embodiment of the gene synthesis device provided in the embodiment of the utility model, Figure 1 the base 4 is arranged on the lower side of the liquid collecting box 2 and connected with the liquid collecting box 2, and the motor assembly 301 is connected with the base 4 to support the entire device.
[0050] It should be noted that the gene synthesis device provided in the embodiment of the utility model realizes the synthesis and drainage functions of the reagent in the synthesis column 101 through the turnover of the plate 307, thereby supporting the synthesis device to perform multiple rounds of synthesis operation.
[0051] The working principle is as follows: the upper sealing surface of the sealing plate 307 is attached to the lower plane of the synthetic substrate 104 by turning the sealing plate 307 upward, the channel between the synthetic column 101 and the liquid collecting box 2 is closed, the lower through hole of the synthetic column 101 is sealed, and the conditions for the synthesis work are provided. The sealing plate 307 is separated from the synthetic substrate 104 by turning the sealing plate 307 downward, the channel between the synthetic column 101 and the liquid collecting box 2 is opened, the liquid in the synthetic column 101 flows to the upper surface of the sealing plate 307 by gravity or negative pressure, and flows to the liquid collecting box 2 through the inclined surface. In this process, the upper surface of the sealing plate 307 avoids liquid residue, and when the sealing plate 307 is turned upward again, it prevents liquid from entering the synthetic column 101, avoids causing reagent pollution, and supports the smooth progress of the synthesis and liquid discharge work.
[0052] The above merely describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims
1. A gene synthesizer apparatus characterized by comprising: The application relates to a synthesis device, which comprises a synthesis assembly, a liquid collecting box, a sealing plate and a driving assembly. The synthesis assembly comprises a synthesis base plate and a heating base plate, wherein the heating base plate is arranged on the upper side of the synthesis base plate and connected with the synthesis base plate; the synthesis column passes through the heating base plate and the synthesis base plate and is connected with the heating base plate and the synthesis base plate. The synthesis base plate further comprises a heat insulation pad, which is arranged on the upper side of the heating base plate and is in close contact with the heating base plate, and the synthesis column passes through the heating base plate. The driving assembly comprises a rocker arm, a pushing block and a driving mechanism. The rocker arm is arranged on the lower side of the end of the sealing plate away from the rotating shaft and is rotatably connected with the liquid collecting box.
2. The apparatus according to claim 1, wherein The pushing block is arranged on the lower side of the sealing plate and is connected with the side of the rocker arm facing the rotating shaft, and is adapted to rotate with the rocker arm to push the sealing plate to be in close contact with or separated from the bottom of the synthesis column.
3. The apparatus according to claim 2, wherein The driving mechanism is connected with the rocker arm and is used to drive the rocker arm to rotate.
4. The apparatus according to claim 1, wherein The end of the pushing block away from the rocker arm is in arc structure. The end of the rocker arm away from the pushing block is provided with a sliding groove, which extends along the length direction of the rocker arm. The driving mechanism comprises a motor assembly and a crank. The motor assembly is fixedly arranged.
5. The apparatus according to claim 4, wherein One end of the crank is connected with the power output end of the motor assembly, and the other end is arranged in the sliding groove and has the freedom of rotating in the sliding groove and sliding along the length direction of the sliding groove.
6. The apparatus according to claim 4, wherein The motor assembly is connected with a base arranged on the lower side of the liquid collecting box and connected with the liquid collecting box. The base is arranged on the lower side of the liquid collecting box and connected with the liquid collecting box. The motor assembly is connected with the base.
7. The apparatus according to claim 6, wherein