Stretchable cell culture plug-in

Abstract

The utility model relates to a stretchable cell culture plug-in which comprises a plug-in body, the plug-in body is of an annular structure, the bottom of the plug-in body of the annular structure is connected with a transparent elastic thin film, the upper portion of the plug-in body is connected with a buckle along the outer side, the lower portion of the plug-in body is connected with an O-shaped sealing ring along the outer side, and the plug-in body is provided with a clamping groove. The side wall of the plug-in body is connected with a side wall channel penetrating through the O-shaped sealing ring, the bottom of the side wall channel protrudes out of the bottom of the plug-in body, the thickness of the transparent elastic film ranges from 0.1 mm to 1 mm, and the inner diameter of the side wall channel ranges from 1 mm to 3 mm. The dynamic culture device can meet dynamic culture requirements, and is simple in structure and convenient to operate.

Description

Technical Field

[0001] This utility model belongs to the field of biomedical engineering, and in particular relates to a stretchable cell culture plug. Background Technology

[0002] With the development of cell biology and organoid technology, three-dimensional cell culture technology is gradually replacing traditional two-dimensional cell culture technology. Currently, many cell types possess strong self-assembly capabilities, such as pluripotent stem cells, tumor cells, and tissue cells. Three-dimensional cell spheres are three-dimensional aggregates formed by the self-assembly of various cells, more closely resembling the structural morphology of in vivo tissue cells and more conducive to the study of their functional mechanisms. Therefore, three-dimensional cell spheres can be used in numerous biological and biomedical research fields, such as developmental biology, pathology, pharmacology, and cancer treatment.

[0003] A search revealed the patent: Cell Culture Unit and Cell Culture Apparatus (CN202321349258.3). The cell culture unit includes a substrate and a plug-in. The substrate has a receiving cavity, a first liquid reservoir, a second liquid reservoir, a first flow channel, and a second flow channel. The first flow channel connects the receiving cavity and the first liquid reservoir, and the second flow channel connects the receiving cavity and the second liquid reservoir. The first and second flow channels are arranged vertically. The plug-in is located in the receiving cavity and is detachably connected to the substrate. The plug-in includes a membrane structure, a first culture chamber, and an opening. The membrane structure is located between the first and second flow channels. The first culture chamber communicates with the first flow channel through the opening and with the receiving cavity through filter pores on the membrane structure. This structure is relatively complex and cannot be compatible with a variety of cell culture plates, resulting in insufficient culture adaptability.

[0004] In view of the above-mentioned shortcomings, the designer actively researched and innovated in order to create a new type of stretchable cell culture plug with greater industrial application value. Utility Model Content

[0005] To solve the above-mentioned technical problems, the purpose of this utility model is to provide a stretchable cell culture plug.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A stretchable cell culture plug includes a plug body with a ring-shaped structure. A transparent elastic film is connected to the bottom of the ring-shaped plug body. A buckle is connected to the upper side of the plug body along the outer edge. An O-ring is connected to the lower side of the plug body along the outer edge. A sidewall channel penetrating the O-ring is connected to the sidewall of the plug body. The bottom of the sidewall channel protrudes from the bottom of the plug body. The thickness of the transparent elastic film is 0.1-1 mm, and the inner diameter of the sidewall channel is 1-3 mm.

[0008] Preferably, the stretchable cell culture insert has an outer diameter that is adapted to a 6-well, 12-well, 24-well, or 48-well cell culture plate.

[0009] Preferably, in the stretchable cell culture insert, the depth of the pores in the cell culture plate is greater than the height of the insert body, and the height difference between the two is 1-10 mm.

[0010] Preferably, in the stretchable cell culture plug, the top of the sidewall channel protrudes from the top of the plug body, and the top of the sidewall channel is connected to a through hole, a pagoda connector, or a Luer connector.

[0011] Preferably, the stretchable cell culture plug is made by injection molding of a polymer material, including but not limited to PS, PC, or PMMA.

[0012] Preferably, in the stretchable cell culture plug, the plug body and the elastic film are connected by adhesive bonding, thermoforming, or laser welding.

[0013] Preferably, in the stretchable cell culture insert, the elastic film is made of transparent silicone.

[0014] Preferably, in the stretchable cell culture insert, the outer diameter of the insert body is adapted to a 12-well cell culture plate, wherein the thickness of the elastic film is 0.5 mm, the difference between the depth of the pores of the cell culture plate and the height above the insert body is 5 mm, and the inner diameter of the sidewall channel is 1 mm and connected to the pagoda connector structure.

[0015] Preferably, in the stretchable cell culture insert, the outer diameter of the insert body is adapted to a 24-well cell culture plate, wherein the thickness of the elastic film is 0.5 mm, the difference between the depth of the pores of the cell culture plate and the height above the insert body is 3 mm, and the inner diameter of the sidewall channel is 1 mm and connected to the pagoda connector structure.

[0016] Preferably, in the stretchable cell culture insert, the outer diameter of the insert body is adapted to a 6-well cell culture plate, wherein the thickness of the elastic film is 0.5 mm, the difference between the depth of the pores of the cell culture plate and the height above the insert body is 5 mm, and the inner diameter of the sidewall channel is 1 mm and connected to the pagoda connector structure.

[0017] By means of the above solution, this utility model has at least the following advantages:

[0018] This invention is simple to operate, uses gas to simulate various dynamic microenvironments, and the plug can be adapted to existing cell culture plates, thus it is compatible with most existing detection equipment and is easy to use.

[0019] The above description is only an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0020] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a schematic diagram of the structure of this utility model;

[0022] Figure 2 This is a schematic diagram of the use of this utility model. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0024] Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0025] Example

[0026] like Figure 1As shown, a stretchable cell culture insert includes an insert body 10, which has a ring-shaped structure. A transparent elastic film 101 is connected to the bottom of the ring-shaped insert body 10. A buckle 102 is connected to the upper side of the insert body 10 along the outer side, and an O-ring 103 is connected to the lower side of the insert body 10 along the outer side. A sidewall channel 104 penetrating the O-ring 103 is connected to the sidewall of the insert body 10. The bottom of the sidewall channel 104 protrudes from the bottom of the insert body 10. The thickness of the transparent elastic film 101 is 0.1-1 mm, and the inner diameter of the sidewall channel 104 is 1-3 mm. The depth of the pores of the cell culture plate is higher than the height of the insert body 10, and the height difference between the two is 1-10 mm.

[0027] The outer diameter of the plug-in body 10 described in this invention is adapted to 6-well, 12-well, 24-well, or 48-well cell culture plates, thus improving applicability.

[0028] The top end of the side wall channel 104 described in this utility model protrudes from the top of the plug-in body 10, and the top end of the side wall channel 104 is connected to a through hole, a pagoda connector, or a Luer connector.

[0029] The plug-in body 10 described in this utility model is injection molded from a polymer material, which includes, but is not limited to, PS, PC or PMMA.

[0030] In this invention, the plug-in body 10 and the elastic film 101 are connected by adhesive bonding, hot pressing, or laser welding, with adhesive bonding being the most common method.

[0031] The elastic film 101 described in this invention is made of transparent silicone.

[0032] like Figure 2 As shown, the working principle of this utility model is as follows:

[0033] (1) Select a suitable cell culture plate and place the plug body 10 vertically in one hole 20 of the cell culture plate. At this time, the "O" seal ring 103 will form a sealed chamber 201 between the bottom surface of the transparent elastic film and the bottom of the plate. The side wall channel 104 becomes the only air outlet of the sealed chamber.

[0034] (2) Surface modification of the elastic film at the bottom of the plug-in body using extracellular matrix;

[0035] (3) Inoculate the required cells into the insert and let it stand so that the cells adhere to the upper surface of the elastic membrane;

[0036] (4) After cell adhesion and growth, connect an adjustable frequency gas pump (positive or negative pressure) to the top of the channel. The transparent elastic film 101 can be stretched by the change of gas pressure, thereby realizing the dynamic culture requirements.

[0037] Example 1

[0038] Based on the embodiments, in this utility model, the outer diameter of the plug-in body 10 is adapted to the 12-well cell culture plate. The thickness of the elastic film 101 is 0.5 mm, the difference between the depth of the pores of the cell culture plate and the height above the plug-in body 10 is 5 mm, and the inner diameter of the side wall channel is 1 mm and connected to the pagoda connector structure.

[0039] Example 2

[0040] Based on the embodiments, in this utility model, the outer diameter of the plug-in body 10 is adapted to the 24-well cell culture plate. The thickness of the elastic film 101 is 0.5 mm, the difference between the depth of the pores of the cell culture plate and the height above the plug-in body 10 is 3 mm, and the inner diameter of the side wall channel is 1 mm and connected to the pagoda connector structure.

[0041] Example 3

[0042] Based on the embodiments, in this utility model, the outer diameter of the plug-in body 10 is adapted to the 6-well cell culture plate. The thickness of the elastic film 101 is 0.5 mm, the difference between the depth of the pores of the cell culture plate and the height above the plug-in body 10 is 5 mm, and the inner diameter of the side wall channel is 1 mm and connected to the pagoda connector structure.

[0043] Example 4

[0044] Based on Example 1, and in conjunction with specific implementation methods, the specific operations are as follows:

[0045] (1) Select a 12-well cell culture plate and vertically place the plug body 10 in one of the wells 20 of the cell culture plate. At this time, the “O” seal ring 10 will form a sealed chamber 201 between the bottom surface of the transparent elastic film and the bottom of the well plate. At this time, the side wall channel 104 becomes the only air outlet of the sealed chamber.

[0046] (2) Surface modification of the transparent elastic film at the bottom of the plug-in using collagen (5mg / ml);

[0047] (3) Inoculate intestinal epithelial cells (Caco-2) into the insert and let it stand so that the cells adhere to the upper surface of the elastic membrane;

[0048] (4) After cell adhesion growth, an adjustable frequency gas pump is connected to the top of the sidewall channel. The gas pump operates at a frequency of 30 times / minute, causing the transparent elastic film 101 to deform downward under the gas pressure, thereby simulating a dynamic microenvironment.

[0049] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0050] In the description of this application, it should be noted that the terms "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use. They are used only for the convenience of describing this application and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0051] Furthermore, terms such as "horizontal" and "vertical" do not imply that components must be absolutely horizontal or vertical, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0052] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set up," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0053] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A stretchable cell culture plug, comprising a plug body (10), characterized in that: The plug body (10) has a ring structure. A transparent elastic film (101) is connected to the bottom of the ring structure plug body (10). A buckle (102) is connected to the upper side of the plug body (10) along the outer side. An "O" ring seal (103) is connected to the lower side of the plug body (10) along the outer side. A side wall channel (104) that penetrates the "O" ring seal (103) is connected to the side wall of the plug body (10). The bottom of the side wall channel (104) protrudes from the bottom of the plug body (10). The thickness of the transparent elastic film (101) is 0.1-1 mm, and the inner diameter of the side wall channel (104) is 1-3 mm.

2. The stretchable cell culture plug according to claim 1, characterized in that: The outer diameter of the plug body (10) is adapted to 6-well, 12-well, 24-well or 48-well cell culture plates.

3. The stretchable cell culture plug according to claim 2, characterized in that: The depth of the pores in the cell culture plate is greater than the height of the insert body (10), with a height difference of 1-10 mm.

4. The stretchable cell culture plug according to claim 1, characterized in that: The top of the sidewall channel (104) protrudes from the top of the plug body (10), and the top of the sidewall channel (104) is connected to a through hole, a pagoda connector or a Luer connector.

5. A stretchable cell culture plug according to claim 1, characterized in that: The plug-in body (10) is injection molded from a polymer material, including but not limited to: PS, PC or PMMA.

6. The stretchable cell culture plug according to claim 1, characterized in that: The plug body (10) and the elastic film (101) are connected by adhesive, hot pressing or laser welding.

7. The stretchable cell culture plug according to claim 1, characterized in that: The elastic film (101) is made of transparent silicone.

8. A stretchable cell culture insert according to any one of claims 1 to 7, characterized in that: The outer diameter of the plug body (10) is adapted to the 12-well cell culture plate, wherein the thickness of the elastic film (101) is 0.5 mm, the difference between the depth of the pore diameter of the cell culture plate and the height above the plug body (10) is 5 mm, and the inner diameter of the side wall channel is 1 mm and connected to the pagoda connector structure.

9. A stretchable cell culture insert according to any one of claims 1 to 7, characterized in that: The outer diameter of the plug body (10) is adapted to the 24-well cell culture plate, wherein the thickness of the elastic film (101) is 0.5 mm, the difference between the depth of the pore diameter of the cell culture plate and the height above the plug body (10) is 3 mm, and the inner diameter of the side wall channel is 1 mm and connected to the pagoda connector structure.

10. A stretchable cell culture plug according to any one of claims 1 to 7, characterized in that: The outer diameter of the plug body (10) is adapted to the 6-well cell culture plate, wherein the thickness of the elastic film (101) is 0.5 mm, the depth of the pores of the cell culture plate is 5 mm higher than the height of the plug body (10), and the inner diameter of the side wall channel is 1 mm and connected to the pagoda connector structure.

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