Dynamic cell culture plate

Abstract

The utility model relates to a dynamic cell culture plate which comprises a dynamic cell culture plate body, a plurality of independent dynamic culture areas are arranged on the dynamic cell culture plate body, culture solution pools are arranged at the left end and the right end of each dynamic culture area, and an inlet and an outlet are formed in the centers of the two culture solution pools. A dynamic culture channel is connected between the two culture solution pools, the dynamic culture channel is communicated with the culture solution pools through inlets and outlets, the dynamic culture channel comprises an upper-layer cell culture chamber, a middle-layer elastic film and a lower-layer gas driving chamber, and the elastic film is arranged between the cell culture chamber and the gas driving chamber; and the gas driving chamber is communicated with gas driving equipment through a pipeline. The device is high in flux and simple to operate, and can be driven by gas to simulate various dynamic motion microenvironments.

Description

Technical Field

[0001] This utility model belongs to the field of biomedical engineering, and in particular relates to a dynamic cell culture plate. Background Technology

[0002] With the development of cell biology and organoid technology, three-dimensional cell culture technology is gradually replacing traditional two-dimensional cell culture technology. Currently, many cell types possess strong self-assembly capabilities, such as pluripotent stem cells, tumor cells, and tissue cells. Three-dimensional cell spheres are three-dimensional aggregates formed by the self-assembly of various cells, more closely resembling the structural morphology of in vivo tissue cells and more conducive to the study of their functional mechanisms. Therefore, three-dimensional cell spheres can be used in numerous biological and biomedical research fields, such as developmental biology, pathology, pharmacology, and cancer treatment.

[0003] A search revealed a patent: a dynamic cell culture plate (CN201821730096.7). This dynamic cell culture plate includes a tray, cell culture wells, a drive device, a support base, a drive shaft, gears, a connecting rod, a culture medium tank, pre-drilled holes, and an impeller. In use, activating the drive device causes the gears and connecting rods to rotate via the drive shaft. The impeller at the end of the connecting rod rotates accordingly, causing the culture medium to flow within the culture medium tank. This creates a dynamic environment for the cells in the culture wells, achieving dynamic cell culture. However, this method is relatively complex for cell culture and has limited practicality.

[0004] In view of the above-mentioned shortcomings, the designer has actively researched and innovated in order to create a new type of dynamic cell culture plate with greater industrial application value. Utility Model Content

[0005] To address the aforementioned technical problems, the purpose of this invention is to provide a dynamic cell culture plate.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A dynamic cell culture plate includes a dynamic cell culture plate with multiple independent dynamic culture areas. Each dynamic culture area has a culture medium pool at both ends. An inlet / outlet is located at the center of each of the two culture medium pools. A dynamic culture channel connects the two culture medium pools and communicates with the culture medium pools through the inlet / outlet. The dynamic culture channel includes an upper cell culture chamber, a middle elastic membrane, and a lower gas-driven chamber. An elastic membrane is provided between the cell culture chamber and the gas-driven chamber. The gas-driven chamber is connected to a gas-driven device through a pipe.

[0008] Preferably, in the dynamic cell culture plate, the cell culture chamber has a width of 0.05-3 cm, a height of 0.02-3 cm, and a length equal to the distance between the centers of the two end culture medium pools.

[0009] Preferably, in the aforementioned dynamic cell culture plate, the elastic film has a thickness of 0.1-2 mm and is made of transparent silicone.

[0010] Preferably, in the dynamic cell culture plate, the gas-driven chamber has a width of 0.05-3 cm and a height of 0.02-3 cm.

[0011] Preferably, in the dynamic cell culture plate, the pipe has a connection hole that communicates with a gas-driven device.

[0012] Preferably, the dynamic cell culture plate is formed by injection molding of a polymer material, including but not limited to: PS, PC or PMMA.

[0013] Preferably, in the dynamic cell culture plate, the arrangement of the cell culture plate and the culture medium pool (101) is adapted to a 24-well culture plate, a 48-well culture plate, or a 96-well culture plate.

[0014] Preferably, in the dynamic cell culture plate, the cell culture chamber has a width of 0.5 cm and a height of 0.5 cm, and the elastic film has a thickness of 0.5 mm; the gas-driven chamber has a width of 0.5 cm and a height of 0.5 cm.

[0015] Preferably, in the aforementioned dynamic cell culture plate, the cell culture chamber has a width of 0.5 cm and a height of 0.1 cm, and the elastic film has a thickness of 0.2 mm; the gas-driven chamber has a width of 0.5 cm and a height of 0.6 cm.

[0016] Preferably, in the aforementioned dynamic cell culture plate, the cell culture chamber has a width of 1.5 cm and a height of 0.5 cm, and the elastic film has a thickness of 1.0 mm; the gas-driven chamber has a width of 1.5 cm and a height of 0.7 cm.

[0017] By means of the above solution, this utility model has at least the following advantages:

[0018] This invention features high throughput and simple operation. It utilizes gas-driven simulation to create various dynamic microenvironments, and its culture plate structure is compatible with existing cell culture plates, making it compatible with most existing detection equipment and providing a user-friendly experience.

[0019] The above description is only an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0020] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a schematic diagram of the structure of this utility model;

[0022] Figure 2 This is a partial enlarged view of utility model A. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0024] Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0025] Example

[0026] like Figure 1 and Figure 2As shown, a dynamic cell culture plate includes a dynamic cell culture plate 10, on which multiple independent dynamic culture zones 100 are provided. Each dynamic culture zone 100 has a culture medium pool 101 at both its left and right ends. An inlet / outlet is located at the center of each of the two culture medium pools 101. A dynamic culture channel connects the two culture medium pools 101, communicating with the culture medium pools 101 through the inlet / outlet. The dynamic culture channel includes an upper cell culture chamber 102, a middle elastic membrane 103, and a lower gas layer. An elastic membrane 103 is provided between the driving chamber 104, the cell culture chamber 102, and the gas-driven chamber 104. The gas-driven chamber 104 is connected to the gas-driven device through a pipe 105. The cell culture chamber 102 has a width of 0.05-3 cm, a height of 0.02-3 cm, and a length equal to the distance between the centers of the two culture medium pools. The elastic membrane 103 has a thickness of 0.1-2 mm and is made of transparent silicone. The gas-driven chamber 104 has a width of 0.05-3 cm and a height of 0.02-3 cm.

[0027] The pipe 105 described in this utility model has a connection hole 106 that communicates with a gas-driven device.

[0028] The cell culture plate 10 described in this invention is formed by injection molding of a polymer material, including but not limited to: PS, PC or PMMA.

[0029] The arrangement of the cell culture plate 10 and culture medium pool 101 described in this invention is adapted to 24-well, 48-well, or 96-well culture plates, thereby improving applicability.

[0030] Example 1

[0031] Based on the embodiments, in this utility model, the cell culture chamber 102 has a width of 0.5 cm and a height of 0.5 cm, the elastic film 103 has a thickness of 0.5 mm, and the gas-driven chamber 104 has a width of 0.5 cm and a height of 0.5 cm.

[0032] Example 2

[0033] Based on the embodiments, in this utility model, the cell culture chamber 102 has a width of 0.5 cm and a height of 0.1 cm, the elastic film 103 has a thickness of 0.2 mm, and the gas-driven chamber 104 has a width of 0.5 cm and a height of 0.6 cm.

[0034] Example 3

[0035] Based on the embodiments, in this utility model, the cell culture chamber 102 has a width of 1.5 cm and a height of 0.5 cm, the elastic film 103 has a thickness of 1.0 mm, and the gas-driven chamber 104 has a width of 1.5 cm and a height of 0.7 cm.

[0036] Example 4

[0037] Based on the structure of any one of Examples 1 to 3, the specific operation for intestinal epithelial cells is as follows:

[0038] (1) Sterilization treatment of dynamic cell culture plates;

[0039] (2) The upper surface of the elastic film in the organ-on-a-chip was modified with collagen (0.5 mg / ml);

[0040] (3) Add the required intestinal epithelial cells (Caco-2) into the upper cell culture chamber through the cell seeding well (inlet and outlet), and let stand so that the cells adhere to the upper surface of the elastic membrane;

[0041] (4) Add the cell culture medium to the culture medium pool;

[0042] (5) After Caco-2 cells adhere and grow, connect the culture plate to an adjustable frequency gas pump. After turning on the gas pump, apply pressure to the lower gas drive chamber at a frequency of 30 times / minute. This causes the middle layer film to deform downward under the gas pressure, thereby simulating a dynamic microenvironment.

[0043] Example 5

[0044] Based on the structure of any one of Examples 1 to 3, the specific operations for cardiomyocytes are as follows:

[0045] (1) Sterilize dynamic cell culture plates;

[0046] (2) The upper surface of the elastic film in the organ-on-a-chip was modified with collagen (0.5 mg / ml);

[0047] (3) Add the required iPSCs-induced differentiated cardiomyocytes into the upper cell culture chamber through the cell seeding well, and let them stand so that the cells adhere to the upper surface of the elastic membrane.

[0048] (4) Add the cell culture medium to the culture medium pool;

[0049] (5) After the iPSCs-induced differentiated cardiomyocytes adhere and grow, the culture plate is connected to an adjustable frequency gas pump. After the gas pump is turned on, pressure is applied to the lower gas drive chamber at a frequency of 60 times / minute, so that the intermediate membrane deforms downward under the action of gas pressure, thereby simulating a dynamic microenvironment.

[0050] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0051] In the description of this application, it should be noted that the terms "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use. They are used only for the convenience of describing this application and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0052] Furthermore, terms such as "horizontal" and "vertical" do not imply that components must be absolutely horizontal or vertical, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0053] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set up," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0054] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A dynamic cell culture plate, characterized in that: The system includes a dynamic cell culture plate (10), which has multiple independent dynamic culture areas (100). Each dynamic culture area (100) has a culture medium pool (101) at both ends. An inlet and outlet are provided at the center of the two culture medium pools (101). A dynamic culture channel is connected between the two culture medium pools (101). The dynamic culture channel is connected to the culture medium pools (101) through the inlet and outlet. The dynamic culture channel includes an upper cell culture chamber (102), a middle elastic membrane (103), and a lower gas-driven chamber (104). An elastic membrane (103) is provided between the cell culture chamber (102) and the gas-driven chamber (104). The gas-driven chamber (104) is connected to a gas-driven device through a pipe (105).

2. The dynamic cell culture plate according to claim 1, characterized in that: The cell culture chamber (102) has a width of 0.05-3 cm, a height of 0.02-3 cm, and a length equal to the distance between the centers of the two culture medium pools.

3. The dynamic cell culture plate according to claim 1, characterized in that: The elastic film (103) has a thickness of 0.1-2 mm and is made of transparent silicone.

4. A dynamic cell culture plate according to claim 1, characterized in that: The width of the gas-driven chamber (104) is 0.05-3 cm and the height is 0.02-3 cm.

5. A dynamic cell culture plate according to claim 1, characterized in that: The pipe (105) is provided with a connection hole (106) that communicates with the gas-driven device.

6. A dynamic cell culture plate according to claim 1, characterized in that: The cell culture plate (10) is formed by injection molding of a polymer material, including but not limited to: PS, PC or PMMA.

7. A dynamic cell culture plate according to claim 1, characterized in that: The arrangement of the cell culture plate (10) and culture medium pool (101) is adapted to a 24-well culture plate, a 48-well culture plate or a 96-well culture plate.

8. A dynamic cell culture plate according to any one of claims 1 to 7, characterized in that: The cell culture chamber (102) has a width of 0.5 cm and a height of 0.5 cm, and the elastic membrane (103) has a thickness of 0.5 mm; the gas-driven chamber (104) has a width of 0.5 cm and a height of 0.5 cm.

9. A dynamic cell culture plate according to any one of claims 1 to 7, characterized in that: The cell culture chamber (102) has a width of 0.5 cm and a height of 0.1 cm, and the elastic membrane (103) has a thickness of 0.2 mm; the gas-driven chamber (104) has a width of 0.5 cm and a height of 0.6 cm.

10. A dynamic cell culture plate according to any one of claims 1 to 7, characterized in that: The cell culture chamber (102) has a width of 1.5 cm and a height of 0.5 cm, and the elastic membrane (103) has a thickness of 1.0 mm; the gas-driven chamber (104) has a width of 1.5 cm and a height of 0.7 cm.

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