Adjustable batch sample embedding mold

Abstract

The utility model provides an adjustable batch sample embedding mold. The adjustable batch sample embedding mold comprises an embedding frame, a plurality of baffles, a plurality of sealing assemblies and a plurality of locking assemblies, according to the embedding mold, the embedding frame can be divided into a plurality of spaces by utilizing the movable baffles, so that biological tissue samples can be conveniently placed in batches. In addition, an extraction assembly is arranged, so that the process of removing the embedded sample from the embedding frame is simplified, and the integrity of the sample is ensured. When the whole embedded sample is placed on the slicing machine base, a plurality of independent tissue sample slices can be obtained at the same time, and the efficiency of the experiment process is remarkably improved. The whole embedded sample is placed on a slicing machine base, and a plurality of separated tissue sample slices can be obtained at the same time. The device is convenient to operate, the time required for embedding and slicing a plurality of samples is greatly shortened, the experiment efficiency is improved, meanwhile, the problem that a single sample is not stably fixed on a slicing machine due to the fact that the size of the embedded single sample is too small is solved, and the accuracy and the reliability of an experiment result are ensured.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental instruments, and in particular to an adjustable batch sample embedding mold. Background Technology

[0002] Tissue embedding refers to the process of immersing a biological tissue sample, after fixation, dehydration, and clearing, in an embedding medium (such as paraffin or resin), which then solidifies to form a firm block. The purpose of embedding is to protect the integrity and stability of the tissue sample, while also facilitating subsequent sectioning.

[0003] In existing technologies, the embedding process generally includes taking a preheated embedding frame and a metal plate, assembling an embedding mold, pouring a small amount of molten wax into the embedding frame as a bottom support, placing the prepared tissue block with the cut side down at the bottom of the frame to ensure the tissue block is stable during the embedding process, continuing to add molten wax to the upper edge of the embedding frame to ensure the tissue block is completely covered, and when the surface molten wax solidifies to a certain thickness, slowly immersing the embedding frame in cold water (ice water can be used in summer) for rapid solidification. After the wax block has completely solidified, the embedding frame is pushed open, and the wax block is removed for subsequent processing.

[0004] However, in practice, biological tissues need to be embedded individually to avoid multiple biological tissues being mixed together, which would affect experimental observation and analysis. If there are multiple biological tissues, they need to be embedded multiple times, which is quite troublesome. Moreover, the commonly used embedding frames are 1.5cm x 1.5cm in length and width, and 0.3cm-0.6cm in height, resulting in a small tissue sample volume. When the sample is cut into sections on a microtome, it is unstable, especially when the sample is cut in half, it becomes very unstable due to the small bottom area of ​​the sample. Utility Model Content

[0005] In view of the shortcomings of the prior art described above, the purpose of this utility model is to provide an adjustable batch sample embedding mold, which can at least solve the problem of the inconvenience of batch embedding biological tissues in the prior art.

[0006] To achieve the above and other related objectives, this utility model provides an adjustable batch sample embedding mold, including an embedding frame, several baffles, several sealing components, and several locking components;

[0007] The side wall of the embedding frame is provided with several longitudinally arranged insertion holes;

[0008] Each baffle is inserted into the side wall of the embedding frame through a corresponding insertion hole and extends into the interior of the embedding frame, dividing the interior space of the embedding frame;

[0009] The sealing assembly is used to prevent liquid inside the embedding frame from leaking from the insertion holes on the side wall of the embedding frame;

[0010] The locking assembly is used to fix the baffle to the embedding frame.

[0011] In one embodiment, each of the sockets is rectangular, and each of the sockets is provided with a sealing ring.

[0012] In one embodiment, both the embedding frame and the baffle are made of transparent material.

[0013] Preferably, scales are provided on the side of the baffle and the side wall of the embedding frame.

[0014] In one embodiment, the baffle is rectangular and adapted to the socket (10).

[0015] Preferably, the front end of the baffle is provided with a handle, and the handle is connected to a label plate.

[0016] In one embodiment, the sealing assembly includes plugs, each of which can be matched with a socket.

[0017] Preferably, each end of the plug is provided with a first connector, and each end of the insertion hole is provided with a first connecting groove that matches the first connector.

[0018] In one embodiment, the locking assembly includes a protrusion connected to the embedding frame. Opposing clamping plates are provided on both sides of the protrusion. A bidirectional screw and a crossbar are provided at the front end of the protrusion. The bidirectional screw drives the two clamping plates to move synchronously, and the crossbar passes through both clamping plates and the protrusion. In one embodiment, the two clamping plates are L-shaped, with their bottoms facing each other, and their opposing sides used to fix the baffle.

[0019] Furthermore, both sides facing each other are covered with anti-slip mats.

[0020] In one embodiment, the bottom of the embedding frame is provided with an extraction component, the extraction component includes a base plate, the base plate is laid flat on the bottom surface of the embedding frame, a second connector is provided at the center of the lower surface of the base plate, and the bottom of the embedding frame is provided with a second connecting hole that matches the second connector.

[0021] Preferably, the bottom of the base plate is provided with a sealing gasket and several supporting members, the bottom of the embedding frame is provided with a third recessed hole that matches the supporting members, the side of the base plate is provided with an upwardly extending scraper, and the sealing gasket and the scraper are respectively wrapped around both ends of the base plate.

[0022] More preferably, the lower end of the second connector is provided with a third connecting hole for connecting to an external crank handle.

[0023] This utility model has the following beneficial effects:

[0024] This invention presents an adjustable batch sample embedding mold that divides the embedding frame into multiple spaces for easy batch placement of biological tissue samples. It also includes an extraction component for easy removal of the embedded samples from the embedding frame, improving work efficiency. After the samples are fixed, the entire embedded sample can be glued to the microtome base, simultaneously obtaining multiple separate tissue sample sections. This adjustable batch sample embedding mold is easy to operate, significantly saving time in embedding multiple samples and sectioning, improving efficiency, and also avoiding the problem of small embedded samples being unstable on the microtome. Attached Figure Description

[0025] Figure 1 This is a perspective view of an adjustable batch sample embedding mold according to the present invention.

[0026] Figure 2 This is a perspective view of the embedding frame of an adjustable batch sample embedding mold according to an embodiment of the present invention.

[0027] Figure 3 This is a perspective view of the baffle of an adjustable batch sample embedding mold in one embodiment of the present invention.

[0028] Figure 4 This is a perspective view of the sealing component of an adjustable batch sample embedding mold in one embodiment of the present invention.

[0029] Figure 5 An adjustable batch sample embedding mold in one embodiment of this utility model Figure 1 Enlarged view of section A in the middle;

[0030] Figure 6 An exploded view of the locking assembly of an adjustable batch sample embedding mold in one embodiment of this utility model;

[0031] Figure 7 This is a cross-sectional view of the extraction component of an adjustable batch sample embedding mold in one embodiment of the present invention;

[0032] Figure 8 This is a perspective view of the extraction component of an adjustable batch sample embedding mold in one embodiment of the present invention.

[0033] Figure 9 The image shows a bottom-view perspective view of the extraction component of an adjustable batch sample embedding mold in one embodiment of this utility model.

[0034] Figure 10 This is a sectional perspective view of the first connecting member of an adjustable batch sample embedding mold in one embodiment of the present invention.

[0035] In the picture:

[0036] 1-Embedded frame; 10-Insertion hole; 11-Sealing ring; 12-First connecting groove; 13-Second connecting hole; 14-Third recessed hole;

[0037] 2-Baffle; 21-Handle; 22-Label plate;

[0038] 3-Sealing assembly; 30-Plug; 31-First connector;

[0039] 4-Locking assembly; 40-Protrusion; 41-Double-acting screw; 42-Clamping plate; 43-Crossbar;

[0040] 5-Extraction component; 50-Base plate; 51-Second connector; 52-Sealing gasket; 53-Support component; 54-Scraper. Detailed Implementation

[0041] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification.

[0042] Please see Figures 1 to 10 It should be understood that the structures, proportions, sizes, etc., illustrated in the accompanying drawings are merely for illustrative purposes to aid those skilled in the art and are not intended to limit the scope of this invention. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in proportions, or adjustments to size, without affecting the effectiveness and purpose of this invention, should still fall within the scope of the disclosed technical content. Furthermore, the terms "upper," "lower," "left," "right," "middle," and "one" used in this specification are merely for clarity and not intended to limit the scope of this invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of this invention.

[0043] like Figure 1-6 As shown, this utility model provides an adjustable batch sample embedding mold, including an embedding frame 1, several baffles 2, several sealing components 3, and several locking components 4.

[0044] The embedding frame 1 is generally about 70*50*40mm in size, which meets the length of the blade on the microtome and the vertical movement of the microtome. Its front sidewall has several longitudinally arranged insertion holes 10, each hole 10 being the same size and evenly distributed. Each baffle 2 can be inserted into the sidewall of the embedding frame 1 through an insertion hole 10, thus dividing the interior of the embedding frame 1 into several independent spaces. After the baffle 2 is inserted into the embedding frame 1, a gap remains between its rear end and the rear sidewall of the embedding frame 1 to facilitate the flow of the embedding agent. The sealing assembly 3 is used to prevent liquid (e.g., embedding agent) inside the embedding frame 1 from leaking from the insertion holes 10 on the sidewall of the embedding frame 1. The locking assembly 4 is used to fix the baffle 2 in a certain position in the embedding frame 1, preventing the baffle 2 from shifting when the biological tissue sample is moved, thereby avoiding mixing of adjacent biological tissues.

[0045] For one embodiment, please refer to Figure 1-3 Each insertion hole 10 is rectangular, matching the rectangular baffle 2 to facilitate the insertion and movement of the baffle 2. Each insertion hole 10 is equipped with a sealing ring 11, which typically surrounds the insertion hole 10. The outer wall of the sealing ring 11 has a groove surrounding it, allowing the edge of the insertion hole 10 to fit precisely within the groove. Preferably, the sealing ring 11 is made of an elastic material such as silicone to ensure a tight seal after the baffle 2 is inserted into the insertion hole 10, preventing leakage of the embedding agent from the insertion hole 10 when it is added.

[0046] For one embodiment, please refer to Figure 1-3 Both the embedding frame 1 and the baffle 2 are made of transparent material, and scales are provided on the side of the baffle 2 and the side wall of the embedding frame 1. The scales are painted with a darker color to contrast with the transparent color of the baffle 2 and the embedding frame 1, facilitating observation. The scales allow for adjustment of the sample's position when placing biological samples, ensuring that the front / back and left / right positions of the sample are as flat as possible.

[0047] For one embodiment, please refer to Figure 1-3 To match the square embedding frame 1, the baffle 2 is rectangular. The baffle 2 and the embedding frame 1 work together to facilitate the division of the internal space of the embedding frame 1, thereby effectively distinguishing biological tissue samples. Furthermore, the front end of the baffle 2 is equipped with a handle 21, which allows researchers to manually push or pull to adjust the insertion depth of the baffle 2 into the embedding frame 1. The handle 21 is connected to a label plate 22, allowing for the recording of sample information (such as sample origin and embedding time) by writing sample information on a label and attaching it to the label plate 22.

[0048] For one embodiment, please refer to Figure 1 and Figure 4When the biological tissue is large, one or more baffles 2 need to be removed to create a larger space, facilitating the placement of the larger biological tissue. The insertion holes 10 left after removing the baffles 2 need to be sealed using a sealing assembly 3. The sealing assembly 3 includes a plug 30, the size of which matches each insertion hole 10. The plug 30 is a protrusion with the same height and thickness as the baffle 2, used to insert into the insertion hole 10 to replace the position of the baffle 2 and prevent embedding agent leakage. Both ends of the plug 30 are provided with first connectors 31, and both ends of each insertion hole 10 are provided with first connecting grooves 12 that match the first connectors 31. Preferably, the first connectors 31 are fastening bolts, and the first connecting grooves 12 are correspondingly set as threaded grooves. In use, the plug 30 is inserted into the insertion hole 10, two fastening bolts are passed through both sides of the plug 30, and tools are used to fix the fastening bolts in the threaded grooves, thereby completing the sealing operation at the insertion hole 10.

[0049] For one embodiment, please refer to Figure 1 , Figure 5 and Figure 6 The locking assembly 4 includes a protrusion 40 connected to the embedding frame 1. The protrusion 40 has an L-shaped cross-section, with the shorter side of the L facing downwards. Opposing clamping plates 42 are provided on both sides of the protrusion 40. A bidirectional screw 41 and a crossbar 43 are provided at the front end of the protrusion 40. The bidirectional screw 41 is rotatably connected to the protrusion 40 and its outer surface is threadedly connected to the clamping plates 42. The bidirectional screw 41 is used to drive the two clamping plates 42 to move synchronously. The crossbar 43 passes through both clamping plates 42 and the protrusion 40 simultaneously. The crossbar 43 is fixedly connected to the protrusion 40 and slidably connected to the clamping plates 42.

[0050] In use, by rotating one end of the bidirectional screw 41 (both ends of the bidirectional screw 41 can be equipped with corresponding knobs), the bidirectional screw 41 rotates, causing the clamping plates 42 to move in opposite directions along the crossbar 43, thus fixing the baffle 2 between the two clamping plates 42. Both clamping plates 42 are designed with an L-shaped structure, and their bottom facing sides are covered with anti-slip pads. The anti-slip pads facilitate the increase of friction between the plates and the baffle 2, thereby achieving the fixing effect. Preferably, the protective pads are rubber pads.

[0051] For one embodiment, please refer to Figure 7-10The bottom of the embedding frame 1 is equipped with an extraction component 5, which is used to push the embedded sample out of the embedding frame 1, improving operational efficiency. The extraction component 5 includes a base plate 50, which is laid flat on the inner bottom surface of the embedding frame 1 to support the embedded sample. In the unused state, the base plate 50 is located below the baffle 2 to avoid interfering with the movement of the baffle 2. A second connector 51 is provided at the center of the lower surface of the base plate 50, and a second connecting hole 13 matching the second connector 51 is provided at the bottom of the embedding frame 1. The second connector 51 has a cylindrical structure and is rotatably connected to the bottom surface of the embedding frame 1. The outer surface of the second connector 51 is provided with a thread. After the second connector 51 is threadedly connected to the second connecting hole 13, it fixes the base plate 50. Below the base plate 50, there is a sealing gasket 52 and several support members 53. The sealing gasket 52 is used to enhance the sealing between the base plate 50 and the bottom of the embedding frame 1 to prevent the embedding agent from leaking from the bottom of the embedding frame 1. The support members 53 are used to support the whole device and enhance the structural stability.

[0052] In one embodiment, there are four support members 53, forming four support legs. The bottom of the embedding frame 1 is provided with a third recess 14 that matches the support member 53. The side of the base plate 50 is provided with an upwardly extending scraper 54 for scraping off the embedding agent adhering to the inner sidewall of the embedding frame 1. The scraper 54 surrounds the base plate 50. The cross-section of the scraper 54 is a right-angled triangle with the inclined surface facing upward. One right-angled side is attached to the sidewall of the embedding frame 1, and the other right-angled side is fixedly connected to the base plate 50. The sealing gasket 52 surrounds the base plate 50. The lower end of the second connector 51 is provided with a third connecting hole for connecting to an external crank handle. In use, the external crank handle is connected to the second connector 51. Turning the crank handle causes the second connector 51 to rotate, separating the second connector 51 from the second connecting hole 13. Then, pushing the crank handle causes the base plate 50 to rise through the second connector 51, pushing the embedded sample out of the embedding frame 1. During the rise of the base plate 50, the scraper 54 scrapes off the embedding agent adhering to the inner wall of the embedding frame 1.

[0053] The specific usage process of this utility model is as follows: Insert the baffle 2 into the embedding frame 1 (creating several fluid-connected spaces within the embedding frame 1), place multiple biological tissue samples from different sources into the frame (ensuring that the biological tissue samples are completely isolated from each other by the baffle 2, so that after the samples are fixed, they will not come into contact with each other; only the embedding agent between the partially sealed parts will solidify into one, avoiding sample mixing), use the locking assembly 4 to fix the baffle 2, and adjust the position of the biological tissue samples according to the scale. Use the sealing assembly 3 to seal the unused insertion holes 10, and pour in the embedding agent. After the samples are fixed, remove the baffle 2. Connect and rotate the second connector 51 through the crank handle to separate the second connector 51 from the embedding frame 1, and push the crank handle to push the base plate 50 upward through the second connector 51, pushing the embedded sample out of the embedding frame 1. Then, fix the embedded sample as a whole on the microtome base for slicing, thereby obtaining multiple separated tissue sample slices.

[0054] In summary, compared with existing technologies, it has the following beneficial effects:

[0055] 1. This utility model proposes an adjustable batch sample embedding mold that can divide the embedding frame into multiple spaces for easy batch placement of biological tissue samples. It also includes an extraction component to facilitate the removal of embedded samples from the embedding frame, improving work efficiency. After the samples are fixed, the entire embedded sample can be glued to the microtome base, simultaneously obtaining multiple separate tissue sample sections. This adjustable batch sample embedding mold is easy to operate, significantly saving time in embedding multiple samples and sectioning, improving efficiency, and also avoiding the problem of small embedded samples being unstable on the microtome.

[0056] 2. This utility model provides an adjustable batch sample embedding mold. Through a movable baffle and the insertion holes on the side wall of the embedding frame, the embedding frame can be divided into multiple spaces, which facilitates the batch placement of biological tissue samples and achieves the purpose of batch embedding of biological tissues.

[0057] 3. This utility model provides an adjustable batch sample embedding mold. By setting an extraction component at the bottom of the embedding frame, the process of removing embedded samples from the embedding frame is simplified, improving operational efficiency and ensuring sample integrity. When the entire embedded sample is placed on the slicer base, multiple independent tissue sample slices can be obtained simultaneously, significantly improving the efficiency of the experimental process.

[0058] 4. This utility model provides an adjustable batch sample embedding mold. During subsequent operations, the embedded sample can be fixed to the microtome base using glue, simultaneously obtaining multiple separate tissue sample sections. Furthermore, this adjustable batch sample embedding mold is convenient to operate, significantly reducing the time required for embedding and sectioning multiple samples, improving experimental efficiency. It also solves the problem of unstable fixation on the microtome due to the small volume of a single embedded sample, ensuring the accuracy and reliability of experimental results.

[0059] The above embodiments are merely illustrative of the principles and effects of this utility model and are not intended to limit the scope of this utility model. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of this utility model. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this utility model should still be covered by the claims of this utility model.

Claims

1. An adjustable batch sample embedding mold, characterized in that, The embedding mold includes: an embedding frame (1), several baffles (2), several sealing components (3), and several locking components (4); The embedding frame (1) has several longitudinally arranged insertion holes (10) on its side wall; each baffle (2) is inserted into the side wall of the embedding frame (1) through the corresponding insertion hole (10) and extends into the interior of the embedding frame (1) to divide the interior space of the embedding frame (1); the sealing assembly (3) is used to prevent the liquid in the embedding frame (1) from leaking from the insertion holes (10) on the side wall of the embedding frame (1); the locking assembly (4) is used to fix the baffle (2) on the embedding frame (1); The side of the baffle (2) and the side wall of the embedding frame (1) are both equipped with scales. The scales are set so that the position of the biological sample can be adjusted according to the scales around it when placing the sample, ensuring that the front, back and left or right positions of the sample are flat.

2. The embedding mold according to claim 1, characterized in that: Each of the sockets (10) is rectangular, and each of the sockets (10) is provided with a sealing ring (11).

3. The embedding mold according to claim 1, characterized in that: Both the embedding frame (1) and the baffle (2) are made of transparent material.

4. The adjustable batch sample embedding mold according to claim 1, characterized in that: The baffle (2) is rectangular and is adapted to the socket (10).

5. The embedding mold according to claim 1, characterized in that: The sealing assembly (3) includes a plug (30), each of which can be matched with a socket (10).

6. The embedding mold according to claim 1, characterized in that: The locking assembly (4) includes a protrusion (40) connected to the embedding frame (1), and the two sides of the protrusion (40) are respectively provided with opposing clamping plates (42). The front end of the protrusion (40) is provided with a bidirectional screw (41) and a crossbar (43). The bidirectional screw (41) is used to drive the two clamping plates (42) to move synchronously, and the crossbar (43) passes through the two clamping plates (42) and the protrusion (40) at the same time.

7. The embedding mold according to claim 6, characterized in that: Both clamps (42) are L-shaped structures with their bottoms facing each other, and their opposing sides are used to fix the baffle (2).

8. The embedding mold according to claim 1, characterized in that: The bottom of the embedding frame (1) is provided with an extraction component (5), which includes a base plate (50). The base plate (50) is laid flat on the bottom surface of the embedding frame (1). A second connector (51) is provided at the center of the lower surface of the base plate (50). The bottom of the embedding frame (1) is provided with a second connecting hole (13) that matches the second connector (51).

9. The embedding mold according to claim 8, characterized in that: The bottom plate (50) is provided with a sealing gasket (52) and several support members (53). The bottom of the embedding frame (1) is provided with a third recess (14) that matches the support member (53). The side of the bottom plate (50) is provided with an upwardly extending scraper (54). The scraper (54) surrounds the bottom plate (50). The cross-section of the scraper (54) is a right triangle with the inclined surface facing upward. One right-angled side is attached to the side wall of the embedding frame (1), and the other right-angled side is fixedly connected to the bottom plate (50). The sealing gasket (52) surrounds the bottom plate (50).

10. The embedding mold according to claim 8, characterized in that: The second connector (51) has a third connecting hole at the lower center for connecting to an external crank handle.

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