Liquid path system of gene sequencing mechanism

Abstract

The utility model discloses a liquid path system of a gene sequencing mechanism. The liquid path system comprises a reagent bin, a blending transmission pipe group and a heat preservation mechanism, the number of the reagent bins is two, reagent tubes are arranged at the upper ends of the reagent bins, and a blending bin is placed in front of the two reagent bins; the blending transmission pipe group comprises main pipelines, connecting tee joints and first blending pipelines, the main pipelines are arranged at output ports of the reagent bins, the connecting tee joints are mounted at the front ends of the main pipelines through flanges, and the first blending pipelines are mounted at the ends, close to each other, of the two connecting tee joints through flanges; according to the gene sequencing mechanism liquid path system, different reagents can be independently conveyed, the different reagents can also be conveyed after being mixed, meanwhile, when special reagents are conveyed, the temperature in the reagent conveying pipelines can be kept, the situation that the activity of the reagents is reduced due to low temperature is avoided, and the stability of the reagents is improved. And the result of gene sequencing work is influenced.

Description

Technical Field

[0001] This utility model relates to the field of medical equipment technology, specifically to a liquid circuit system for a gene sequencing institution. Background Technology

[0002] Gene sequencing plays a crucial role in disease diagnosis, detecting gene mutations in hereditary diseases and tumors, providing a basis for early diagnosis and precision treatment. Furthermore, gene sequencing can be used in drug development, predicting drug efficacy and adverse reactions by analyzing a patient's genetic information, thus enabling personalized drug therapy.

[0003] In traditional gene sequencing, if different reagents are required, they are usually delivered to a separate delivery tube. If different reagents need to be mixed together, the user needs to mix them externally before pouring them into the tube for delivery.

[0004] There are some problems. The operation is cumbersome when different reagents need to be mixed, and the activity of some special reagents will decrease at low temperatures. Individual transmission pipelines often do not have the function of keeping the temperature warm, thus reducing the efficiency of sequencing work. In this case, workers have to mix these reagents. To address this, we propose a liquid circuit system for gene sequencing institutions. Utility Model Content

[0005] The technical problem to be solved by this utility model is to overcome the existing defects and provide a liquid circuit system for gene sequencing institutions. Different reagents can be transported separately or mixed before transport. At the same time, when transporting special reagents, the internal temperature of the reagent transport pipeline can be maintained to avoid the reduction of reagent activity due to low temperature, which would affect the results of gene sequencing. This can effectively solve the problems in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a liquid circuit system for a gene sequencing institution, including a reagent compartment, a dispensing and transfer tube assembly, and a temperature-preserving mechanism;

[0007] Reagent compartments: There are two of them, and each reagent compartment is equipped with a reagent tube at the top. A dispensing compartment is placed in front of the two reagent compartments.

[0008] The reagent delivery pipeline assembly includes a main pipeline, connecting tees, and a first delivery pipeline. Each reagent compartment has a main pipeline at its output port. A connecting tee is installed at the front end of each main pipeline via a flange. Two connecting tees are connected to a first delivery pipeline via flanges at their closest ends. The front end of each first delivery pipeline penetrates the rear wall of the delivery compartment. A partition component is connected in series at the rear end of each first delivery pipeline to isolate its internal flow path. The outer arc surface of each first delivery pipeline is equipped with a heat-insulating mechanism. Different reagents can be delivered individually or mixed before delivery. Furthermore, when delivering special reagents, the internal temperature of the reagent delivery pipeline can be maintained to prevent low temperatures from reducing reagent activity and affecting the gene sequencing results.

[0009] Furthermore, it also includes a microcontroller, which is located behind the two reagent compartments. The input terminal of the microcontroller is electrically connected to an external power supply for stable control.

[0010] Furthermore, the isolation component is a distribution valve, which is connected in series at the rear end of the first distribution pipeline. The input end of the distribution valve is electrically connected to the output end of the microcontroller, which facilitates opening or closing the internal path of the first distribution pipeline.

[0011] Furthermore, the mixing and transmission pipeline group also includes output pipelines and isolation valves. The output pipelines are all installed at the opposite ends of two connecting tees via flanges. The rear end of each output pipeline is connected in series with an isolation valve. The input end of each isolation valve is electrically connected to the output end of a microcontroller, which facilitates the individual transmission of different reagents or the transmission to the same mixing chamber.

[0012] Furthermore, the insulation mechanism includes an insulation board and a heating wire. The outer arc surface of the output pipe of the first dispensing pipe is wrapped with an insulation board, and the outer arc surface of the insulation board is wound with a heating wire. The input end of the heating wire is electrically connected to the output end of the microcontroller to maintain the temperature inside the dispensing and transmission pipe group.

[0013] Furthermore, a temperature sensor is installed on the upper side of the mixing chamber. The output of the temperature sensor is electrically connected to the output of the microcontroller, and the input of the temperature sensor is electrically connected to an external power supply, which facilitates monitoring of the ambient temperature.

[0014] Furthermore, it also includes a transfer pump and a dispensing output pump. The transfer pumps are connected in series in the middle of the main pipeline. The front end of the dispensing chamber is provided with a second dispensing pipeline. The middle of the second dispensing pipeline is connected in series with a dispensing output pump. The input ends of the dispensing output pump and the transfer pump are both electrically connected to the output end of the microcontroller, ensuring stable reagent transfer.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows: The liquid circuit system of this gene sequencing institution has the following advantages:

[0016] The combination of the barrier valve and the mixing valve inside the mixing and delivery tube assembly allows different reagents to flow into the mixing chamber when they need to be mixed. At the same time, it can maintain the temperature inside the tube assembly to prevent the reagent activity from decreasing due to low temperature, which would reduce the efficiency of gene sequencing. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of this utility model;

[0018] Figure 2 This is a schematic diagram of the rear side of the present invention;

[0019] Figure 3 This is a schematic diagram of the front side plane of the insulation mechanism of this utility model.

[0020] In the diagram: 1. Reagent compartment, 2. Mixing and transfer tubing assembly, 21. Main pipe, 22. Connecting tee, 23. Output pipe, 24. First mixing pipe, 25. Barrier valve, 26. Mixing valve, 3. Insulation mechanism, 31. Insulation board, 32. Heating wire, 4. Transfer pump, 5. Reagent tube, 6. Microcontroller, 7. Mixing compartment, 8. Second mixing pipe, 9. Mixing output pump, 10. Temperature sensor. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Please see Figure 1-3 This embodiment provides a technical solution: a liquid circuit system for a gene sequencing institution, including a reagent compartment 1, a dispensing and transfer tube assembly 2, and a temperature-preserving mechanism 3;

[0023] Reagent compartment 1: There are two of them. Each reagent compartment is equipped with a reagent tube 5 at the top and also includes a microcontroller 6. The microcontroller 6 is located behind the two reagent compartments 1. The input terminal of the microcontroller 6 is electrically connected to an external power supply. A mixing compartment 7 is placed in front of the two reagent compartments 1. A temperature sensor 10 is installed on the upper side of the mixing compartment 7. The output terminal of the temperature sensor 10 is electrically connected to the output terminal of the microcontroller 6, and the input terminal of the temperature sensor 10 is electrically connected to an external power supply.

[0024] Dispensing and transmission pipeline assembly 2 includes a main pipeline 21, a connecting tee 22, and a first dispensing pipeline 24. The output port of reagent chamber 1 is equipped with a main pipeline 21. The front end of each main pipeline 21 is fitted with a connecting tee 22 via a flange. The ends of two connecting tees 22 closest to each other are fitted with a first dispensing pipeline 24 via flanges. The front end of each first dispensing pipeline 24 penetrates the rear wall of the dispensing chamber 7. The rear end of each first dispensing pipeline 24 is connected in series with a partition assembly to isolate its internal flow. The outer arc surface of each first dispensing pipeline 24 is equipped with a heat insulation mechanism 3. The partition assembly is a dispensing valve 26, which is connected in series to the rear end of the first dispensing pipeline 24. The input end of each dispensing valve 26 is electrically connected to the output end of a microcontroller 6. The dispensing and transmission pipeline assembly 2 also includes a transmission... The outlet pipe 23 and the isolation valve 25 are both installed at opposite ends of two connecting tees 22 via flanges. Isolation valves 25 are connected in series at the rear ends of each outlet pipe 23. The input ends of the isolation valves 25 are electrically connected to the output end of the microcontroller 6. The insulation mechanism 3 includes an insulation board 31 and a heating wire 32. The outer arc surface of the outlet pipe 23 of the first mixing pipe 24 is wrapped with the insulation board 31, which is an inner rock wool board wrapped with stainless steel. Heating wires 32 are wound around the outer arc surface of the insulation board 31. The input ends of the heating wires 32 are electrically connected to the output end of the microcontroller 6. It also includes a transfer pump 4 and a mixing output pump 9. The transfer pump 4 is connected in series in the middle of the main pipe 21. A second mixing pipe 8 is provided at the front end of the mixing chamber 7. A dispensing output pump 9 is connected in series in the middle of pipe 8. The input terminals of dispensing output pump 9 and transfer pump 4 are both electrically connected to the output terminal of microcontroller 6. Workers add different reagents into the two reagent chambers 1 through reagent tube 5. Then, the worker operates microcontroller 6 to operate dispensing valve 26, closing it so that reagents cannot pass through the first dispensing pipe 24. Next, the worker operates microcontroller 6 to operate blocking valve 25, opening it. Then, the worker operates microcontroller 6 to operate transfer pump 4, which transfers the reagents from the two reagent chambers 1 through main pipe 21 and connecting tee 22 to the output pipe 23. The reagents are then directly discharged into other equipment through output pipe 23. When reagents need to be mixed, the worker operates microcontroller 6... The blocking valve 25 is activated, and when it closes, the reagent cannot pass through the output pipe 23. Then, the operator controls the microcontroller 6 to activate the mixing valve 26, opening it. The reagent flowing through the connecting tee 22 is then blocked by the blocking valve 25 and enters the first mixing pipe 24, subsequently flowing into the mixing chamber 7. After a period of time, the operator controls the microcontroller 6 to activate the mixing output pump 9. The mixing output pump 9 then discharges the mixed reagent from the mixing chamber 7 to another device through the second mixing pipe 8. Note that some special reagents, such as polymerases and other biological enzymes, are highly temperature-sensitive. During gene sequencing, these enzymes require suitable temperatures to achieve optimal activity.To ensure the smooth progress of the sequencing reaction, when the ambient temperature is low, the rock wool inside the insulation plate 31 forms an insulation layer to prevent external cold air from exchanging heat with the internal pipes of the mixing and transfer tube assembly 2, which would affect the reagents during the transfer process. When the external ambient temperature continues to drop, the temperature sensor 10 detects this and transmits a signal to the microcontroller 6. After receiving the signal, the microcontroller 6 sends a control command to the heating wire 32. The heating wire 32 operates and begins to heat the outer stainless steel of the insulation plate 31. After the stainless steel absorbs the heat from the heating wire 32, after a period of time, the internal rock wool plate exchanges heat with the outer stainless steel layer after absorbing the heat. The cotton board then transfers this heat to the inner stainless steel layer. At this point, the temperature of the stainless steel inner layer of the insulation board 31 exceeds the temperature of the internal pipes of the mixing and distribution tube assembly 2. The pipes inside the distribution tube assembly 2 also begin to heat up through heat exchange with the insulation board 31. This increase in internal temperature prevents a decrease in reagent activity due to low temperature, thus avoiding a decline in gene sequencing efficiency. When different reagents need to be mixed, the valves work together to allow different reagents to flow into the mixing chamber, while simultaneously maintaining the internal temperature of the distribution tube assembly 2 to prevent a decrease in reagent activity due to low temperature, which would otherwise reduce gene sequencing efficiency.

[0025] The working principle of the liquid circuit system for gene sequencing institutions provided by this utility model is as follows: A worker inserts different reagents into the two reagent chambers 1 through reagent tube 5. Then, the worker operates the microcontroller 6 to activate the dispensing valve 26, closing it so that the reagents cannot pass through the first dispensing pipe 24. Next, the worker operates the microcontroller 6 to activate the blocking valve 25, opening it. Then, the worker operates the microcontroller 6 to activate the transfer pump 4, which transfers the reagents from the two reagent chambers 1 through the main pipe 21 and connecting tee 22 to the output pipe 23. At this point, the reagents are directly discharged through the output pipe 23. When reagents need to be mixed in other equipment, the worker operates the microcontroller 6 to activate the blocking valve 25, closing it and preventing reagents from passing through the output pipe 23. Then, the worker operates the microcontroller 6 to activate the mixing valve 26, opening it. The reagents flowing through the tee 22 are then blocked by the blocking valve 25 and enter the first mixing pipe 24, subsequently entering the mixing chamber 7. After a period of time, the worker operates the microcontroller 6 to activate the mixing output pump 9, which then uses the second mixing pipe 8 to mix the reagents inside the mixing chamber 7. The reagents are discharged to another device. Some special reagents, such as polymerases and other biological enzymes, are highly sensitive to temperature. During gene sequencing, these enzymes require a suitable temperature to achieve optimal activity and ensure the smooth progress of the sequencing reaction. When the ambient temperature is low, the rock wool inside the insulation plate 31 forms an insulation layer to prevent external cold air from exchanging heat with the internal pipes of the dispensing and transfer tube assembly 2, which could affect the reagents during the transfer process. When the external ambient temperature continues to decrease, the temperature sensor 10 detects this and transmits a signal to the microcontroller 6. Upon receiving the signal, the microcontroller 6 sends a signal to the heating wire 32. A control command is issued, and the heating wire 32 operates and begins to heat the outer stainless steel of the insulation plate 31. After the stainless steel absorbs the heat from the heating wire 32, after a period of time, the inner rock wool board exchanges heat with the outer stainless steel after absorbing the heat. Then, the rock wool board transfers this heat to the inner stainless steel. At this time, the temperature of the inner stainless steel of the insulation plate 31 exceeds the temperature of the internal pipes of the distribution and transfer tube group 2. The internal pipes of the distribution and transfer tube group 2 also begin to heat up through heat exchange with the insulation plate 31. At this time, the internal temperature of the internal pipes of the distribution and transfer tube group 2 increases, which avoids the reduction of reagent activity due to low temperature, thus preventing a decrease in the efficiency of gene sequencing.

[0026] It is worth noting that the transfer pump 4 and the distribution output pump 9 disclosed in the above embodiments can be model MSAF070P, the temperature sensor 10 can be model WZPB-241, the barrier valve 25 and the distribution valve 26 can be model Q911F-16P, and the microcontroller 6 can be model S7-200. The microcontroller 6 controls the operation of the transfer pump 4, the distribution output pump 9, the barrier valve 25 and the distribution valve 26 using methods commonly used in the prior art.

[0027] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A liquid circuit system for a gene sequencing facility, characterized in that: It includes a reagent compartment (1), a dispensing and transfer tube assembly (2), and a heat preservation mechanism (3); Reagent compartment (1): There are two of them. Each reagent compartment (1) is equipped with a reagent tube (5) at the top. A mixing compartment (7) is placed in front of the two reagent compartments (1). The mixing and transmission pipe assembly (2) includes a main pipe (21), a connecting tee (22) and a first mixing pipe (24). The output port of the reagent chamber (1) is provided with a main pipe (21). The front end of the main pipe (21) is connected to a connecting tee (22) through a flange. The two connecting tees (22) are connected to a first mixing pipe (24) through a flange at their closest ends. The front end of the first mixing pipe (24) penetrates the rear side wall of the mixing chamber (7). The rear end of the first mixing pipe (24) is connected in series with a partition component for blocking its internal flow. The outer arc surface of the first mixing pipe (24) is provided with a heat preservation mechanism (3).

2. The liquid circuit system of the gene sequencing facility according to claim 1, characterized in that: It also includes a microcontroller (6), which is located behind the two reagent compartments (1), and the input terminal of the microcontroller (6) is electrically connected to an external power supply.

3. The liquid circuit system of the gene sequencing facility according to claim 2, characterized in that: The isolation component is a regulating valve (26), and the regulating valve (26) is connected in series at the rear end of the first regulating pipe (24). The input end of the regulating valve (26) is electrically connected to the output end of the microcontroller (6).

4. The liquid circuit system of the gene sequencing facility according to claim 2, characterized in that: The distribution and transmission pipeline group (2) also includes an output pipeline (23) and a blocking valve (25). The output pipeline (23) is installed at the opposite ends of two connecting tees (22) through flanges. The rear end of the output pipeline (23) is connected in series with a blocking valve (25). The input end of the blocking valve (25) is electrically connected to the output end of the microcontroller (6).

5. The liquid circuit system of the gene sequencing facility according to claim 4, characterized in that: The heat preservation mechanism (3) includes a heat preservation plate (31) and a heating wire (32). The outer arc surface of the output pipe (23) of the first distribution pipe (24) is wrapped with the heat preservation plate (31). The outer arc surface of the heat preservation plate (31) is wrapped with the heating wire (32). The input end of the heating wire (32) is electrically connected to the output end of the microcontroller (6).

6. The liquid circuit system of the gene sequencing facility according to claim 2, characterized in that: A temperature sensor (10) is installed on the upper side of the mixing chamber (7). The output end of the temperature sensor (10) is electrically connected to the output end of the microcontroller (6), and the input end of the temperature sensor (10) is electrically connected to an external power supply.

7. The liquid circuit system of the gene sequencing facility according to claim 2, characterized in that: It also includes a transfer pump (4) and a dispensing output pump (9). The transfer pump (4) is connected in series in the middle of the main pipeline (21). The front end of the dispensing chamber (7) is provided with a second dispensing pipeline (8). The dispensing output pump (9) is connected in series in the middle of the second dispensing pipeline (8). The input ends of the dispensing output pump (9) and the transfer pump (4) are both electrically connected to the output end of the microcontroller (6).

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