Extraction device for full-automatic nucleic acid extraction, amplification and detection

Abstract

The utility model provides an extraction device for full-automatic nucleic acid extraction, amplification and detection, and relates to the technical field of nucleic acid extraction and amplification, the extraction device comprises a bottom frame, the middle part of the upper end of the bottom frame is provided with a chassis, the bottom of the chassis is provided with a Peltier, the bottom of the Peltier is provided with a water cooling disc, and the middle part of the upper end of the chassis is annularly provided with a plurality of groups of inner ring detection jacks; the peripheries of the inner ring detection jacks are correspondingly provided with outer ring detection jacks, and the tops of the inner ring detection jacks and the outer ring detection jacks are respectively provided with an inner ring gear member and an outer ring gear member which are of a hollow structure. According to the nucleic acid extraction amplification detection equipment, the structure of the nucleic acid extraction amplification detection equipment is simplified, the size of the equipment is reduced, the cost of the equipment is reduced, and the driven gear is arranged between the inner ring gear piece and the outer ring gear piece, so that the inner ring gear piece and the outer ring gear piece are driven to rotate in the same direction; therefore, convenience is provided for extraction and amplification of nucleic acid, and the working efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid extraction and amplification technology, specifically to an extraction device for fully automated nucleic acid extraction, amplification, and detection. Background Technology

[0002] The principle of nucleic acid extraction using magnetic beads is based on the same principle as that of silica membrane centrifuge columns. Nanotechnology is used to modify and enhance the surface of superparamagnetic nanoparticles, preparing superparamagnetic silica nanobeads. These magnetic beads can specifically recognize and efficiently bind to nucleic acid molecules at the microscopic interface. Utilizing the superparamagnetism of silica-coated magnetic nanospheres, DNA and RNA can be separated from samples such as blood, animal tissues, food, and pathogenic microorganisms under the influence of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field. This method has applications in multiple fields, including clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology detection, food safety testing, and molecular biology research. Nucleic acid amplification detection technology involves providing in vitro amplification conditions for nucleic acid fragments, causing them to amplify exponentially. Fluorescent dyes or fluorescent labels are added during the amplification process, and the intensity of the fluorescence signal is detected using optical devices. The results of nucleic acid amplification are obtained through the processing and analysis of the fluorescence signal.

[0003] Currently, the existing nucleic acid extraction process is all done manually in the laboratory. The nucleic acid extraction process requires the use of a nucleic acid extractor, but common nucleic acid extractors have the disadvantages of being large in size and having a small sample capacity.

[0004] To address the aforementioned issues, we propose an extraction device for fully automated nucleic acid extraction, amplification, and detection. Utility Model Content

[0005] To address the problems in the background art, this utility model provides an extraction device for fully automated nucleic acid extraction, amplification, and detection.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0007] An extraction device for fully automated nucleic acid extraction, amplification, and detection includes a base frame, a chassis at the upper middle part of the base frame, a Peltier at the bottom of the chassis, a water-cooling plate at the bottom of the Peltier, and the water-cooling plate being fixedly installed at the upper middle part of the base frame.

[0008] The upper center of the chassis is provided with multiple sets of inner ring detection holes in a ring shape. The outer ring detection holes are provided around the inner ring detection holes. Hollow inner ring gear components and outer ring gear components are respectively provided on the top of the inner ring detection holes and the outer ring detection holes. Magnetic suction components are provided on one side of the inner cavity of the inner ring gear components and the outer ring gear components.

[0009] A drive assembly includes a transmission gear driven by a servo motor. The transmission gear is located in the middle of multiple sets of inner ring gears and meshes with all sets of inner ring gears. The drive assembly is used to drive the multiple sets of inner ring gears and outer ring gears to rotate in the same direction.

[0010] Preferably, a gear mounting plate is provided at the upper center of the chassis. The gear mounting plate is located at the top of the inner ring detection hole and the outer ring detection hole. The transmission gear is movably mounted at the upper center of the gear mounting plate. The inner ring gear and the outer ring gear are both movably mounted on the upper surface of the gear mounting plate and distributed around the transmission gear. The inner ring gear and the outer ring gear are connected by a driven gear.

[0011] Preferably, a gear top cover is provided at the upper center of the gear mounting plate, and the gear top cover is used to limit the top of the inner ring gear, the outer ring gear and the driven gear.

[0012] Preferably, a top plate is provided at the upper center of the gear top cover, and multiple sets of inner ring through holes and multiple sets of outer ring through holes are provided in an annular shape at the inner center of the top plate. The inner ring through holes are correspondingly located at the upper center of the inner ring detection insertion hole, and the outer ring through holes are correspondingly located at the upper center of the outer ring detection insertion hole. A heating element is provided between the inner ring through holes and the outer ring through holes.

[0013] Preferably, the bottom of the water cooling plate is provided with a water inlet pipe and a water outlet pipe.

[0014] Preferably, the servo motor is mounted at the bottom center of the gear mounting plate.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] In this solution, by setting the inner and outer ring gear components into a ring mounting structure, the number of nucleic acid extraction and amplification units is increased, while the structure of the nucleic acid extraction and amplification detection equipment is simplified, the equipment volume is reduced, and the equipment cost is lowered. Furthermore, by setting a driven gear between the inner and outer ring gear components, when the servo motor is started to drive the transmission gear to rotate, the inner and outer ring gear components are driven to rotate in the same direction, thereby providing convenience for nucleic acid extraction and amplification and improving work efficiency.

[0017] In addition, by setting a water-cooling plate at the bottom of the Peltier, the cooling liquid circulates in the water-cooling plate during the nucleic acid amplification process, which effectively improves the uniformity of heat dissipation and cooling at the bottom of the Peltier, thereby improving the quality of nucleic acid extraction and amplification. Attached Figure Description

[0018] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0019] Figure 2 This is an exploded view of the present invention;

[0020] Figure 3 This is a schematic diagram of the inner ring gear component and the outer ring gear component in this utility model;

[0021] Figure 4 This utility model Figure 3 Exploded view;

[0022] Figure 5 This is a schematic diagram of the chassis structure in this utility model;

[0023] Figure 6 This is a bottom structural diagram of the present invention.

[0024] In the diagram: 1. Base frame; 2. Water cooling plate; 21. Inlet pipe; 22. Outlet pipe; 3. Peltier; 4. Chassis; 41. Inner ring detection socket; 42. Outer ring detection socket; 5. Gear mounting plate; 51. Transmission gear; 511. Servo motor; 52. Inner ring gear component; 53. Outer ring gear component; 54. Driven gear; 6. Gear top cover; 7. Top plate; 71. Inner ring through hole; 72. Outer ring through hole; 73. Heating element. Detailed Implementation

[0025] The technical solution in this application embodiment is to solve the problems mentioned in the background art, and the overall idea is as follows:

[0026] Example: Refer to Figure 1 - Figure 6 As shown, an extraction device for fully automated nucleic acid extraction, amplification and detection according to this embodiment includes a base frame 1, a base plate 4 is provided at the middle of the upper end of the base frame 1, a Peltier 3 is provided at the bottom of the base plate 4, a water cooling plate 2 is provided at the bottom of the Peltier 3, and the water cooling plate 2 is fixedly installed at the middle of the upper end of the base frame 1.

[0027] The upper middle part of the chassis 4 is provided with multiple sets of inner ring detection holes 41 in a ring shape. The outer ring detection holes 42 are provided around the inner ring detection holes 41 respectively. Hollow inner ring gear component 52 and outer ring gear component 53 are respectively provided on the top of the inner ring detection holes 41 and the outer ring detection holes 42. Magnetic suction component is provided on one side of the inner cavity of the inner ring gear component 52 and the outer ring gear component 53.

[0028] The drive assembly includes a transmission gear 51, which is driven by a servo motor 511. The transmission gear 51 is located in the middle of multiple sets of inner ring gears 52 and is meshed with all sets of inner ring gears 52. The drive assembly is used to drive the multiple sets of inner ring gears 52 and outer ring gears 53 to rotate in the same direction.

[0029] The servo motor 511 can drive the transmission gear 51 to rotate, thereby driving multiple sets of inner ring gears 52 and multiple sets of outer ring gears 53 to rotate in the same direction. This drives the magnetic attracting components on one side of the inner ring gears 52 and outer ring gears 53 to rotate around the nucleic acid test tube placed in the inner ring detection socket 41 and outer ring detection socket 42, so that the magnetic beads in the nucleic acid test tube are attracted and rotated, thereby completing the nucleic acid extraction process.

[0030] A gear mounting plate 5 is provided at the upper center of the chassis 4. The gear mounting plate 5 is located at the top of the inner ring detection hole 41 and the outer ring detection hole 42. The transmission gear 51 is movably mounted at the upper center of the gear mounting plate 5. The inner ring gear 52 and the outer ring gear 53 are both movably mounted on the upper surface of the gear mounting plate 5 and are distributed around the transmission gear 51. The inner ring gear 52 and the outer ring gear 53 are connected by a driven gear 54. The driven gear 54 meshes with the inner ring gear 52 and the outer ring gear 53 respectively, so that when the inner ring gear 52 rotates, it drives the driven gear 54 to rotate. Then, the rotation of the driven gear 54 drives the outer ring gear 53 to rotate, thereby realizing that the inner ring gear 52 and the outer ring gear 53 rotate in the same direction.

[0031] A gear top cover 6 is provided at the upper center of the gear mounting plate 5. The gear top cover 6 is used to limit the top of the inner ring gear 52, the outer ring gear 53 and the driven gear 54, thereby improving the stability of the inner ring gear 52, the outer ring gear 53 and the driven gear 54 during rotation.

[0032] In some examples, a top plate 7 is provided at the upper center of the gear top cover 6. The inner center of the top plate 7 is provided with multiple sets of inner ring through holes 71 and multiple sets of outer ring through holes 72 in an annular shape. The inner ring through holes 71 are correspondingly provided at the upper center of the inner ring detection insertion hole 41, and the outer ring through holes 72 are correspondingly provided at the upper center of the outer ring detection insertion hole 42. A heating element 73 is provided between the inner ring through holes 71 and the outer ring through holes 72.

[0033] In some examples, the bottom of the water-cooling plate 2 is provided with an inlet pipe 21 and an outlet pipe 22. During the amplification of nucleic acids, the coolant flows into the water-cooling plate 2 through the inlet pipe 21 and then flows out from the outlet pipe 22, thereby forming a water-cooling cycle and achieving uniform heat dissipation and cooling of the bottom of the Peltier 3.

[0034] In some examples, the servo motor 511 is mounted at the bottom center of the gear mounting plate 5.

[0035] The working principle of this utility model is as follows:

[0036] When nucleic acid amplification is required, the nucleic acid test tube is first inserted into the inner ring detection socket 41 and the outer ring detection socket 42 through the inner ring through hole 71 and the outer ring through hole 72. Then, the servo motor 511 is started, which drives the transmission gear 51 to rotate, thereby driving the inner ring gear 52 and the outer ring gear 53 to rotate in the same direction. This causes the magnetic suction component to rotate around the outside of the nucleic acid test tube, so as to adsorb the magnetic beads inside the nucleic acid test tube and drive them to rotate, thereby completing the nucleic acid extraction process.

[0037] In addition, the Peltier 3 is started for heating to amplify the nucleic acid test tube. During the heating process, the water cooling plate 2 located at the bottom of the Peltier 3 begins to circulate and cool, so as to achieve uniform heat dissipation and cooling of the bottom of the Peltier 3, thereby effectively improving the quality of nucleic acid amplification.

[0038] This invention provides convenience for nucleic acid extraction and amplification and improves work efficiency by providing a driven gear 54 between the inner ring gear 52 and the outer ring gear 53. When the servo motor 511 is started to drive the transmission gear 51 to rotate, the inner ring gear 52 and the outer ring gear 53 will rotate in the same direction.

[0039] In addition, by setting a water-cooling plate 2 at the bottom of the Peltier 3, the cooling liquid circulates in the water-cooling plate 2 during the nucleic acid amplification process, thereby effectively improving the uniformity of heat dissipation and cooling at the bottom of the Peltier 3, and thus improving the quality of nucleic acid extraction and amplification.

[0040] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. An extraction device for fully automated nucleic acid extraction, amplification, and detection, characterized in that, Includes a base frame (1), a chassis (4) is provided at the middle of the upper end of the base frame (1), a Peltier (3) is provided at the bottom of the chassis (4), a water cooling plate (2) is provided at the bottom of the Peltier (3), and the water cooling plate (2) is fixedly installed at the middle of the upper end of the base frame (1); The upper middle part of the chassis (4) is provided with multiple sets of inner ring detection holes (41) in a ring shape. The outer ring detection holes (42) are provided around the inner ring detection holes (41). Hollow inner ring gear components (52) and outer ring gear components (53) are respectively provided on the top of the inner ring detection holes (41) and the outer ring detection holes (42). Magnetic suction components are provided on one side of the inner cavity of the inner ring gear components (52) and the outer ring gear components (53). The drive assembly includes a transmission gear (51) driven by a servo motor (511). The transmission gear (51) is located in the middle of multiple sets of inner ring gears (52) and meshes with multiple sets of inner ring gears (52). The drive assembly is used to drive multiple sets of inner ring gears (52) and outer ring gears (53) to rotate in the same direction.

2. The extraction device for fully automated nucleic acid extraction, amplification, and detection according to claim 1, characterized in that, A gear mounting plate (5) is provided at the upper middle part of the chassis (4). The gear mounting plate (5) is located at the top of the inner ring detection hole (41) and the outer ring detection hole (42). The transmission gear (51) is movably mounted at the upper middle part of the gear mounting plate (5). The inner ring gear component (52) and the outer ring gear component (53) are both movably mounted on the upper surface of the gear mounting plate (5) and distributed around the transmission gear (51). The inner ring gear component (52) and the outer ring gear component (53) are connected by a driven gear (54).

3. The extraction device for fully automated nucleic acid extraction, amplification, and detection according to claim 2, characterized in that, A gear top cover (6) is provided at the upper middle part of the gear mounting plate (5). The gear top cover (6) is used to limit the top of the inner ring gear (52), the outer ring gear (53) and the driven gear (54).

4. The extraction device for fully automated nucleic acid extraction, amplification, and detection according to claim 3, characterized in that, A top plate (7) is provided at the upper middle part of the gear top cover (6). The inner middle part of the top plate (7) is provided with multiple sets of inner ring through holes (71) and multiple sets of outer ring through holes (72) in an annular shape. The inner ring through holes (71) are respectively provided at the upper middle part of the inner ring detection socket (41), and the outer ring through holes (72) are respectively provided at the upper middle part of the outer ring detection socket (42). A heating element (73) is provided between the inner ring through holes (71) and the outer ring through holes (72).

5. The extraction device for fully automated nucleic acid extraction, amplification, and detection according to claim 4, characterized in that, The bottom of the water cooling plate (2) is provided with a water inlet pipe (21) and a water outlet pipe (22).

6. The extraction device for fully automated nucleic acid extraction, amplification, and detection according to claim 5, characterized in that, The servo motor (511) is mounted at the bottom center of the gear mounting plate (5).

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