Miniature fermentation tank

Abstract

The utility model discloses a miniature fermentation tank which comprises a stirring structure, a tank body, a tank cover and a tank bottom, the top of the tank body is covered with the tank cover, the tank bottom is in sealed connection with the bottom of the tank body, the stirring structure is arranged in the tank body and rotationally connected with the tank bottom, the stirring structure is provided with a magnetic attraction part, the tank cover is provided with a liquid taking opening, an air inlet pipe and an exhaust pipe, the air inlet pipe is connected with a connecting pipe, and the bottom of the connecting pipe extends into the bottom of the tank body. The cell culture tank has the advantages that the cell culture solution is filled in the tank body, cells are cultured in the cell culture solution, the gas inlet pipe can convey gases such as oxygen into the tank body, other gases generated in the tank body can be exhausted from the exhaust pipe, and after the cells in the tank body are cultured for a period of time, part of liquid can be taken out from the liquid taking opening; meanwhile, a culture solution can be added into the tank body through the liquid taking opening. The multi-batch seed selection tank has the advantages of simple structure, small tank body volume, small reagent amount, low cost and suitability for multi-batch seed selection of different types in a short time.

Description

TECHNICAL FIELD

[0001] The utility model relates to microorganism culture technical field especially a kind of micro-fermenter. BACKGROUND

[0002] In the microorganism culture process, it needs in short time, multiple batches, different kinds of selection, the volume of current fermenter is relatively large, the amount of reagent used is relatively more, cost is relatively high, not suitable for short time, different kinds of multiple batches selection. UTILITY MODEL CONTENTS

[0003] The utility model solves the technical problem in at, aiming at the deficiency of prior art, provide a kind of micro-fermenter.

[0004] To solve the above technical problem, the utility model adopts the following technical solutions.

[0005] A kind of micro-fermenter, including stirring structure, tank body, tank cover and tank bottom;The tank cover covers in the top of tank body, the tank bottom is sealedly connected with the bottom of tank body, the stirring structure is set in tank body and is rotationally connected with tank bottom, the stirring structure is equipped with magnetic attraction piece, the tank cover is equipped with liquid taking port, air inlet pipe and exhaust pipe, the air inlet pipe is connected with connecting pipe, the bottom of connecting pipe extends into the bottom of tank body.

[0006] A preferred scheme is that the micro-fermenter further includes a temperature detector, the probe of the temperature detector extends into the tank body, and the connector of the temperature detector extends out of the tank body through the tank cover.

[0007] A preferred scheme is that the inner side of the tank bottom is provided with a PH detection structure and a dissolved oxygen detection structure.

[0008] A preferred scheme is that the liquid taking port is plugged with a sealing plug.

[0009] A preferred scheme is that the inner wall of the tank body is provided with a spoiler along the height direction.

[0010] A preferred scheme is that the tank cover is threadedly connected with the top of the tank body.

[0011] The micro fermentation tank provided by the embodiment of the utility model has at least the following beneficial effects: the cell culture solution is contained in the tank body, cells are cultured in the cell culture solution, the air inlet pipe can deliver oxygen and other gases into the tank body, other gases generated in the tank body can be discharged from the air outlet pipe, and part of the liquid can be taken out from the liquid taking port after the cells in the tank body are cultured for a period of time; meanwhile, the cell culture solution can also be added into the tank body through the liquid taking port.

[0012] The above description is only a summary of the technical scheme of the utility model, in order to more clearly understand the technical means of the utility model, the content of the specification can be implemented, and in order to make the above and other purposes, characteristics and advantages of the utility model more obvious and easy to understand, the following preferred embodiments are described in detail, and the drawings are as follows. BRIEF DESCRIPTION OF DRAWINGS

[0013] Fig. 1 It is the perspective view of the utility model;

[0014] Fig. 2 It is the sectional view of the utility model;

[0015] Fig. 3 It is the explosion view of the utility model. DETAILED DESCRIPTION

[0016] In order to explain the idea and purpose of the present application, the present application will be further described in combination with the drawings and specific embodiments.

[0017] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terms used in the specification of the application are only for the purpose of describing specific embodiments and are not intended to limit the application; the specification and claims of the application and the above drawing description of the terms "include" and "have" and any modification thereof are intended to cover non-exclusive inclusion. The specification and claims of the application or the above drawings of the terms "first", "second", "left", "right" and the like are used to distinguish different objects, not to describe a specific order.

[0018] Reference to an "embodiment" herein means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are they necessarily mutually exclusive of one another. As will be apparent to those of ordinary skill in the art, embodiments described herein can be combined with other embodiments.

[0019] As shown in Figs. 1-3 embodiments of the application provide a micro-fermentation tank, comprising a stirring structure 1, a tank body 2, a tank cover 3 and a tank bottom 4; the tank cover 3 covers the top of the tank body 2, the tank bottom 4 is sealingly connected with the bottom of the tank body 2, the stirring structure 1 is arranged in the tank body 2 and is rotationally connected with the tank bottom 4, the stirring structure 1 is provided with a magnetic attraction piece 14, the tank cover 3 is provided with a liquid taking port 5, an air inlet pipe 6 and an air outlet pipe 7, the air inlet pipe 6 is connected with a connecting pipe 8, and the bottom of the connecting pipe 8 extends into the bottom of the tank body 2.

[0020] As shown in Figs. 1-3 , the tank body 2 is filled with cell culture solution, cells are cultured in the cell culture solution, the air inlet pipe 6 can transport oxygen and other gases into the tank body 2, other gases generated in the tank body 2 can be discharged from the air outlet pipe 7, and part of the liquid can be taken out through the liquid taking port 5 after the cells in the tank body 2 are cultured for a period of time; at the same time, culture solution can also be added into the tank body 2 through the liquid taking port 5. The stirring structure 1 is provided with a magnetic attraction piece 14, and an external magnetic member is attracted to the magnetic attraction piece 14 on the stirring structure 1, so that when the external magnetic member rotates, the magnetic attraction piece 14 on the stirring structure 1 is driven to rotate.

[0021] As shown in Figs. 1-3 , the micro-fermentation tank further comprises a temperature detector 9, a probe of the temperature detector 9 extends into the tank body 2, and a connector of the temperature detector 9 extends out of the tank body 2 through the tank cover 3. The temperature detector 9 can detect the temperature in the tank body 2, and the temperature in the tank body 2 can be known in real time.

[0022] As shown in Figs. 1-3 , the inner side of the tank bottom 4 is provided with a PH detection structure 10 and a dissolved oxygen detection structure 11. The PH detection structure 10 can detect the PH value of the culture solution in the tank body 2, and the dissolved oxygen detection structure 11 can detect the oxygen content of the culture solution in the tank body 2.

[0023] As shown in Figs. 1-3 , the liquid taking port 5 is plugged with a sealing plug 12. When liquid is not needed to be taken, the sealing plug 12 plugs the liquid taking port 5.

[0024] As shown in Figs. 1-3As shown in the figure, the inner wall of the tank body 2 is provided with a spoiler 13 along the height direction. When the stirring structure 1 rotates, the culture solution collides with the spoiler 13, so that the stirring of the culture solution is more uniform.

[0025] As shown in the figure, the tank cover 3 is threadedly connected with the top of the tank body 2. The tank cover 3 is convenient to open. Figs. 1-3

[0026] The above is the specific embodiment of the present application, it should be pointed out, for those skilled in the ordinary technical personnel in the art, without departing from the principles of the present application, under the premise of, can also make a number of improvements and refinements, these improvements and refinements also regarded as the protection scope of the present application.​

Claims

1. A miniature fermenter, characterized in that, It includes a stirring structure, a tank body, a tank cover, and a tank bottom; the tank cover is closed on the top of the tank body, the tank bottom is sealed to the bottom of the tank body, the stirring structure is disposed in the tank body and rotatably connected to the tank bottom, the stirring structure is provided with a magnetic suction element, the tank cover is provided with a liquid inlet, an air inlet pipe and an air outlet pipe, the air inlet pipe is connected to a connecting pipe, and the bottom of the connecting pipe extends into the bottom of the tank body.

2. The micro fermenter according to claim 1, characterized in that, The micro fermenter also includes a temperature detector, the probe of which extends into the tank, and the connector of which extends out of the tank through the lid.

3. The micro fermenter according to claim 1, characterized in that, The inner side of the tank bottom is equipped with a pH detection structure and a dissolved oxygen detection structure.

4. The micro fermenter according to claim 1, characterized in that, The liquid extraction port is fitted with a sealing plug.

5. The micro fermenter according to claim 1, characterized in that, The inner wall of the tank is equipped with baffles along the height direction.

6. The micro fermenter according to claim 1, characterized in that, The can lid is threadedly connected to the top of the can body.

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