Stem cell cryopreservation ball

Abstract

The utility model provides a stem cell cryopreservation ball, which relates to the technical field of medical instruments and comprises an upper ball body and a lower ball body, the upper ball body and the lower ball body are detachably connected together, the upper ball body and the lower ball body are of a hemispheroid structure, a storage cavity is formed in the lower ball body, a liquid storage cavity is formed in the upper ball body, and a liquid storage cavity is formed in the lower ball body. And the filtering structure is detachably arranged in the lower ball body. The stem cell cryopreservation device solves the problems that the activity is affected due to insufficient contact between stem cells and a protective agent during cryopreservation, the stem cells and the protective agent cannot be directly separated during resuscitation, and the occupied space is large during placement, and the stem cells and the protective agent can be better combined together through the upper ball body and the lower ball body which are arranged, so that a complete ball is formed; the stem cells stored in the cryopreservation balls are more convenient during cryopreservation, the cooling effect is good, cooling is more uniform, the stem cells are more uniformly mixed with a protective agent during cryopreservation due to the spherical shape, and meanwhile the storage space is reduced.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and in particular to a stem cell cryopreservation sphere. Background Technology

[0002] Stem cells, as a type of pluripotent cell naturally present in the human body, also known as seed cells, have a wide range of clinical applications due to their potential for multi-directional differentiation. Therefore, developing a stable and efficient stem cell storage device is essential. Currently, to better maintain the original cellular characteristics and activity of stem cells during storage, the most traditional method is slow freezing / rapid warming. This involves slowly freezing stem cells and then storing them in liquid nitrogen at -196°C, temporarily removing the cells from their growth state. When needed, they are quickly thawed from the liquid nitrogen for application. In laboratory stem cell cryopreservation, a certain amount of cryoprotectant is added to the stem cells, and the cells are resuspended and mixed before being injected into commercially available cell cryopreservation tubes. Following a standardized procedure, the stem cells are slowly frozen and then placed in a liquid nitrogen device for storage. After being frozen in liquid nitrogen, the basic cellular activity of stem cells ceases. However, the freezing and warming processes during cryopreservation and thawing may cause a decrease in various indicators compared to before freezing, and a certain percentage of cell activity may be lost, significantly reducing thawing efficiency. In addition, because cryoprotectants need to be added, when using commercially available cryovials, centrifugation is required during thawing to remove the cryoprotectants from the cells before they can be used clinically.

[0003] Most existing stem cells are stored in cryopreservation tubes. However, the tubular storage method does not allow for sufficient contact between the stem cells and the cryoprotectant, which affects the activity of the stem cells. Furthermore, the cryoprotectant cannot be directly separated from the stem cells during thawing, and the tubes take up a lot of space when placed in the cryopreservation tube. Utility Model Content

[0004] The purpose of this invention is to provide a stem cell cryopreservation sphere that solves the problems of insufficient contact between stem cells and the cryoprotectant during cryopreservation affecting their activity, the inability to directly separate stem cells from the cryoprotectant during thawing, and the large space required for placement.

[0005] The above-mentioned technical objective of this utility model is achieved through the following technical solution:

[0006] A stem cell cryopreservation sphere includes: an upper sphere and a lower sphere;

[0007] The upper sphere and the lower sphere are detachably connected together, and the upper sphere and the lower sphere are hemispherical structures;

[0008] The storage cavity is located inside the lower sphere;

[0009] The storage cavity is arranged in the interior of the upper sphere;

[0010] The filtering structure is detachably arranged in the lower sphere.

[0011] According to the stem cell cryopreservation sphere, the filtering structure is a filter plate, the filter plate is in a circular plate structure, the filter screen on the filter plate only allows the protective agent to pass through, and the filter screen on the filter plate is in a radioactive distribution.

[0012] According to the stem cell cryopreservation sphere, a taking handle is fixedly arranged at the middle position of the surface of the filter plate.

[0013] According to the stem cell cryopreservation sphere, a connecting ring is fixedly arranged on the surface of one end of the lower sphere corresponding to the upper sphere, a threaded strip is arranged on the outer surface of the connecting ring, a clamping ring is fixedly arranged on one end of the upper sphere corresponding to the lower sphere, a threaded groove is arranged in the inner wall of the clamping ring corresponding to the connecting ring, and the threaded strip and the threaded groove are matched with each other.

[0014] According to the stem cell cryopreservation sphere, a placing base is arranged on the end of the lower sphere away from the upper sphere, and the placing base is in a horizontal circular disc structure.

[0015] In conclusion, the stem cell cryopreservation sphere has the following beneficial technical effects:

[0016] The upper sphere and the lower sphere are in a hemispherical structure, the upper sphere and the lower sphere are combined to form a complete cryopreservation sphere, the stem cells are placed in the lower sphere when the stem cells are cryopreserved, the protective agent is then added, the filter plate is covered to combine the upper sphere with the lower sphere, the cryopreservation sphere is shaken to make the stem cells and the protective agent contact each other, the cryopreservation effect of the stem cells is better improved, the filter plate is arranged to better separate the stem cells from the protective agent during thawing, the separation is more convenient, and the space is more saved when the stem cells are placed.

[0017] Additional aspects and advantages of the present application will be described in the following description, and will become apparent from the following description, or will be learned from the practice of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0019] Figure 1 is a whole structure schematic view of an embodiment of the present application;

[0020] Figure 2 is a whole side plan view of an embodiment of the present application;

[0021] Figure 3 is a whole bottom plan view of an embodiment of the present application;

[0022] Figure 4 is a whole structure expanded schematic view of an embodiment of the present application;

[0023] Figure 5 is a bottom expanded structure schematic view of an embodiment of the present application;

[0024] Figure 6 is a filter plate structure schematic view of an embodiment of the present application;

[0025] Figure 7 is a filter plate bottom plan view of an embodiment of the present application.

[0026] Reference signs:

[0027] 1, lower sphere; 10, connecting ring; 11, placing base; 12, storage cavity;

[0028] 2, upper sphere; 20, clamping ring; 21, liquid storage cavity;

[0029] 3, filter plate; 30, taking handle. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical scheme and advantages of the present application more clear, the technical scheme of the present application will be described clearly and completely below in combination with the drawings in the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0031] The technical scheme of the present application will be described below in combination with the embodiments shown in the drawings: Figures 1-7

[0032] A stem cell cryopreservation ball comprises an upper sphere 2 and a lower sphere 1, the upper sphere 2 and the lower sphere 1 are detachably connected together, the upper sphere 2 and the lower sphere 1 are hemispherical structures, a storage cavity 12 is arranged in the interior of the lower sphere 1, a liquid storage cavity 21 is arranged in the interior of the upper sphere 2, and a filter structure is detachably arranged in the lower sphere 1.

[0033] ​It can be understood that: the upper sphere 2 and the lower sphere 1 are hemispherical structures, the upper sphere 2 and the lower sphere 1 are combined and connected together to form a complete ball, a liquid storage cavity 21 is arranged in the upper sphere 2, a storage cavity 12 is arranged in the lower sphere 1, and a filter structure is arranged between the upper sphere 2 and the lower sphere 1, and the space of the storage cavity 12 is greater than the space of the liquid storage cavity 21.

[0034] The stem cell cryopreservation ball provided by the embodiment of the utility model, through the upper sphere 2 and the lower sphere 1, the upper sphere 2 and the lower sphere 1 are hemispherical structures, an integrated cryopreservation ball is formed by connecting the upper sphere 2 and the lower sphere 1, then the stem cells are placed in the lower sphere 1, the protective agent is added, the upper sphere 2 is combined on the lower sphere 1 by covering the filter plate 3, then the cryopreservation ball is shaken to make the stem cells and the protective agent contact, the cryopreservation effect of the stem cells is better, meanwhile, the filter plate 3 is arranged, so that the stem cells and the protective agent can be separated better during thawing, then the separation is more convenient, meanwhile, the space is saved during placement.

[0035] According to the stem cell cryopreservation ball provided by the embodiment of the utility model, the filter structure is a filter plate 3, the filter plate 3 is a circular plate structure, the filter screen on the filter plate 3 only allows the protective agent to pass through, and the filter screen on the filter plate 3 is distributed in a radial manner.

[0036] Figure 4 And Figure 6 A stem cell cryopreservation ball is implemented in the embodiment, the filter structure is a filter plate 3, and the filter structure can also be a filter layer or other structure with a filtering and separating effect, preferably, the filter plate 3, a filter screen is provided on the filter plate 3, and the filter screen is distributed in a radial manner on the filter plate 3, and the filter screen only allows the protective agent to pass through.

[0037] The stem cell cryopreservation ball provided by the embodiment of the utility model, through the filter structure, the filter structure is arranged as a filter plate 3, a filter screen is provided on the filter plate 3, then the protective agent and the stem cells can be separated better during thawing through the filter screen, then the stem cells can be thawed better without mixing with the protective agent, then the use is more convenient, meanwhile, the filter screen only allows the protective agent to pass through, so that the stem cells are not taken out during separation, the separation effect is better, and the leakage does not occur.

[0038] According to the stem cell cryopreservation ball provided by the embodiment of the utility model, a taking handle 30 is fixedly arranged at the middle position of the surface of the filter plate 3.

[0039] Figure 4 And Figure 6The stem cell cryopreservation ball is provided with a taking handle 30 welded in the middle of the surface of the filter plate 3, and the taking handle 30 is a hollow rectangular structure.

[0040] The stem cell cryopreservation ball can conveniently take and place the filter plate 3, and the filter plate 3 can be taken out to conveniently take out and add stem cells.

[0041] The stem cell cryopreservation ball is provided with a taking handle 30 welded in the middle of the surface of the filter plate 3, and the taking handle 30 is a hollow rectangular structure.

[0042] Figure 1 、 Figure 4 and Figure 5 The stem cell cryopreservation ball is provided with a taking handle 30 welded in the middle of the surface of the filter plate 3, and the taking handle 30 is a hollow rectangular structure.

[0043] The stem cell cryopreservation ball can conveniently take and place the filter plate 3, and the filter plate 3 can be taken out to conveniently take out and add stem cells.

[0044] The stem cell cryopreservation ball is provided with a taking handle 30 welded in the middle of the surface of the filter plate 3, and the taking handle 30 is a hollow rectangular structure.

[0045] Figure 2 and Figure 5The stem cell cryopreservation ball is provided with a placing base 11 arranged at the lower ball 1 far away from the upper ball 2, and the placing base 11 is arranged as a horizontal disc structure without edges and corners at the bottom.

[0046] The stem cell cryopreservation ball is provided with a placing base 11 arranged at the lower ball 1 far away from the upper ball 2, and the placing base 11 is arranged as a horizontal disc structure without edges and corners at the bottom.

[0047] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the utility model, and not to limit them; although the utility model has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the utility model.

Claims

1. A stem cell cryopreserved sphere, characterized by, Include: Upper sphere (2) and lower sphere (1); Upper sphere (2) and lower sphere (1), detachable connection together, the upper sphere (2) and lower sphere (1) are hemispherical structure; Storage cavity (12) is opened in the inside of the lower sphere (1); Liquid storage cavity (21) is opened in the inside of the upper sphere (2); Filter structure, detachable setting in the lower sphere (1).

2. The stem cell cryopreserved sphere of claim 1, wherein: The filter structure is filter plate (3), the filter plate (3) is circular plate structure, the filter screen on the filter plate (3) only allows the protective agent to pass through, the filter screen on the filter plate (3) is in the form of radioactive distribution.

3. The stem cell cryopreserved sphere of claim 2, wherein: The middle position of the surface of the filter plate (3) is fixedly provided with a taking handle (30).

4. The stem cell cryopreserved sphere of claim 1, wherein: The lower sphere (1) is fixedly provided with a connecting ring (10) on the surface of one end corresponding to the upper sphere (2), the outer surface of the connecting ring (10) is provided with a threaded strip, the upper sphere (2) is fixedly provided with a clamping ring (20) on one end corresponding to the lower sphere (1), the inner wall of the clamping ring (20) is provided with a threaded groove corresponding to the connecting ring (10), and the threaded strip and the threaded groove are adapted to each other.

5. The stem cell cryopreserved sphere of claim 1, wherein: The end of the lower sphere (1) away from the upper sphere (2) is provided with a placing base (11), and the placing base (11) is a horizontal circular disc structure.

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