Micro-fluidic biochemical reagent disc ventilation structure

Abstract

The utility model relates to a micro-fluidic biochemical reagent disc ventilation structure, and belongs to the technical field of biochemical analyzers, the micro-fluidic biochemical reagent disc ventilation structure comprises a disc body, a first pasting film and a second pasting film, the disc body is provided with a plurality of blind holes, the blind holes are in one-to-one correspondence with exhaust channels on the disc body, and the first pasting film and the second pasting film are arranged on the disc body. The blind holes are located in the ends, away from the reaction chamber, of the corresponding exhaust channels and communicated with the corresponding exhaust channels, the first pasting film covers the disc body and is used for sealing all the open cavities or channels in the disc body to form a closed structure, a plurality of through holes are formed in the first pasting film, the through holes correspond to the blind holes in the disc body one to one, and the through holes are communicated with the blind holes in the disc body. The through holes are communicated with the corresponding blind holes, the second pasting film is arranged on the first pasting film and covers the through holes in the first pasting film, and a plurality of air outlet channels are formed in the face, close to the first pasting film, of the second pasting film. The method has the advantages that manpower is saved, the preparation time before testing is shortened, and the testing efficiency is effectively improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biochemical analysis instruments, in particular to a microfluidic biochemical reagent disc ventilation structure. BACKGROUND

[0002] A biochemical analyzer is a test instrument for determining biochemical and chemical components in clinical blood or other liquid samples. The reagent disc used with the biochemical analyzer is a carrier for biochemical reactions.

[0003] In related technologies, a microfluidic biochemical reagent disc ventilation structure and biochemical test analysis method are disclosed in Chinese patent document CN117233412A. The microfluidic biochemical reagent disc ventilation structure includes a disc body, a disc cover, a sample addition chamber, a diluent quantitative cavity, a sample quantitative cavity, a mixing chamber, an anti-backflow cavity, an annular channel, a reaction chamber, an annular channel, and an anti-backflow assembly. The anti-backflow assembly includes a ventilation component and an anti-cross contamination component. The ventilation component includes an exhaust channel and a through hole. The exhaust channel is provided on the disc body between the through hole and the reaction chamber and is used to connect the through hole and the reaction chamber. The through hole is provided on the disc body between the reaction chamber and the annular channel. A molecular gas-permeable screen is arranged in the through hole. Under the action of centrifugation, the reagent in the annular channel will first enter the reaction chamber through the anti-backflow channel. The reagent will fill the reaction chamber. The gas in the reaction chamber will enter the through hole through the exhaust channel and be released to the outside. The through hole is connected to the outside through the molecular gas-permeable screen.

[0004] In the related technologies described above, before use, the operator needs to insert a molecular gas-permeable screen into each through hole, which is time-consuming and labor-intensive. Practical new type content

[0005] To help save manpower, shorten the preparation time before testing, and effectively improve the testing efficiency, the present application provides a microfluidic biochemical reagent disc ventilation structure.

[0006] The microfluidic biochemical reagent disc ventilation structure provided by the present application adopts the following technical solution:

[0007] The microfluidic biochemical reagent disc ventilation structure comprises a disc body, a first adhesive film and a second adhesive film, a plurality of blind holes are formed in the disc body, the blind holes correspond to exhaust channels on the disc body one by one, the blind holes are located at one end of the corresponding exhaust channels away from the reaction chamber and are in communication with the corresponding exhaust channels, the first adhesive film is arranged on the disc body and is used for sealing each cavity or channel on the disc body to form a closed structure, a plurality of through holes are formed in the first adhesive film, the through holes correspond to the blind holes on the disc body one by one, the through holes are in communication with the corresponding blind holes, and the second adhesive film is arranged on the first adhesive film and covers the plurality of through holes on the first adhesive film. A plurality of air outlet channels are formed in the second adhesive film close to the first adhesive film, the air outlet channels correspond to the through holes one by one, one end of the air outlet channel is in communication with the corresponding through hole, and the other end is in communication with the external atmosphere.

[0008] Preferably, the second adhesive film comprises an adhesive film layer and a glue layer, the glue layer and the adhesive film layer are sequentially arranged away from the first adhesive film, the glue layer is bonded to the first adhesive film, and the air outlet channels are formed in the glue layer.

[0009] Preferably, the thickness of the glue layer is less than 0.2 mm, and the thickness of the adhesive film layer is 0.05-0.15 mm.

[0010] Preferably, the glue layer is double-sided adhesive.

[0011] Preferably, the adhesive film layer and the glue layer are both circular arcs, and the adhesive film layer, the glue layer and the disc body are concentrically arranged.

[0012] Preferably, the depth of the blind hole is equal to the depth of the exhaust channel on the disc body.

[0013] Preferably, the through hole is concentrically arranged with the corresponding blind hole, and the diameter of the through hole is greater than the diameter of the corresponding blind hole.

[0014] Preferably, the first adhesive film is a transparent polycarbonate film.

[0015] Preferably, the first adhesive film is bonded to the disc body.

[0016] In summary, the present application has the following beneficial technical effects:

[0017] During the detection process, under the centrifugal action, the reagent in the annular channel enters the reaction chamber, the reagent fills the reaction chamber, and the gas in the reaction chamber is discharged to the exhaust channel and the blind hole. Since the through hole is in communication with the corresponding blind hole, the gas can enter the air outlet channel through the corresponding through hole, and finally be discharged to the external atmosphere through the air outlet channel. By changing the original molecular air-permeable plug into a physical channel, it is not necessary to plug a plurality of molecular air-permeable plugs before testing, which not only saves manpower, but also shortens the preparation time before testing, and effectively improves the testing efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 is a schematic diagram of the overall structure of an embodiment of the present application.

[0019] Figure 2 is an exploded view of the overall structure of an embodiment of the present application.

[0020] Figure 3 is a partial structure sectional view of an embodiment of the present application.

[0021] Reference signs: 1, disc body; 2, first adhesive film; 3, second adhesive film; 4, blind hole; 5, exhaust passage; 6, through hole; 7, gas outlet passage. DETAILED DESCRIPTION

[0022] The following will be described in detail in combination with Figures 1-3 the present application.

[0023] The present application discloses a microfluidic biochemical reagent disc ventilation structure. Referring to Figure 1 and Figure 2 , the microfluidic biochemical reagent disc ventilation structure comprises a disc body 1, a first adhesive film 2 and a second adhesive film 3, the disc body 1 is circular in shape, a plurality of blind holes 4 are formed on the disc body 1, the blind holes 4 correspond one-to-one to exhaust passages 5 on the disc body 1, the blind holes 4 are located at one end of the corresponding exhaust passages 5 away from the reaction chamber and are in communication with the corresponding exhaust passages 5, and the cross section of the blind holes 4 is circular. The blind hole 4 refers to a closed hole form, the bottom of which is not in communication with the outside world, and only one inlet, that is, the blind hole 4 does not penetrate the disc body 1 in the thickness direction of the disc body 1.

[0024] Referring to Figure 2 and Figure 3 , the first adhesive film 2 is circular in shape and has a diameter that is adapted to the diameter of the disc body 1, the first adhesive film 2 is covered on the surface of the disc body 1 and is used to seal the open cavities or channels on the disc body 1 to form a closed structure; a plurality of through holes 6 are formed on the first adhesive film 2, the cross section of the through holes 6 is circular, the through holes 6 correspond one-to-one to the blind holes 4 on the disc body 1, the through holes 6 are located above the corresponding blind holes 4 and are in communication with the corresponding blind holes 4; specifically, the through holes 6 are concentrically arranged with the corresponding blind holes 4 and the diameter of the through holes 6 is greater than the diameter of the corresponding blind holes 4, so as to facilitate the exhaust of gas. Among them, the number, position and size of the through holes 6 on the first adhesive film 2 are customized according to the blind holes 4 on the disc body 1, so as to be applicable to reagent discs of different specifications.

[0025] Referring to Figure 2 and Figure 3The second film 3 is arranged on the side of the first film 2 away from the disc body 1, and covers the plurality of through holes 6 on the first film 2. A plurality of air outlet channels 7 are arranged on the side of the second film 3 close to the first film 2. The air outlet channels 7 correspond to the through holes 6 one by one. One end of the air outlet channel 7 is in communication with the corresponding through hole 6, and the other end is in communication with the atmosphere. Specifically, the end of the air outlet channel 7 away from the corresponding through hole 6 penetrates through the side of the second film 3 close to the center of the disc body 1, so as to be in communication with the atmosphere.

[0026] In use, the customized first film 2 is arranged on the surface of the disc body 1, so that the through holes 6 on the first film 2 are aligned with the corresponding blind holes 4, and the through holes 6 are in communication with the corresponding blind holes 4. Then, the second film 3 is arranged on the surface of the first film 2, so that the air outlet channels 7 are in communication with the corresponding through holes 6. During detection, when the disc body 1 is subjected to centrifugal force, the reagent in the annular channel enters the reaction chamber. The reagent fills the reaction chamber and discharges the gas in the reaction chamber to the exhaust channel 5 and the blind hole 4. Since the through holes 6 are in communication with the corresponding blind holes 4, the gas can enter the air outlet channel 7 through the corresponding through hole 6, and finally be discharged to the atmosphere through the air outlet channel 7. The original molecular air permeable plug is replaced by a physical channel, so that the operator does not need to insert a plurality of molecular air permeable plugs on the disc body 1 before testing. This not only saves manpower, but also shortens the preparation time before testing, and effectively improves the testing efficiency. The blind hole 4 can accommodate excess reagent in the reaction chamber, so as to prevent the reagent from overflowing from the through hole 6.

[0027] Referring to Figure 2 The first film 2 can be provided with a through hole according to needs. For example, a sample adding port (not shown in the figure) in communication with the sample adding chamber on the disc body 1 is arranged on the first film 2, so as to facilitate adding a test sample to the sample adding chamber. Meanwhile, a plurality of buffer grooves are arranged on the side of the first film 2 close to the disc body 1. The buffer grooves correspond to the reaction chambers on the disc body 1 one by one, and are in communication with the corresponding reaction chambers, which facilitates the mixed sample to enter the reaction chamber on the disc body 1.

[0028] Referring to Figure 2 and Figure 3 The second film 3 includes a film layer and a glue layer. The film layer and the glue layer are both circular arcs, and the film layer, the glue layer and the disc body 1 are concentrically arranged. The glue layer and the film layer are sequentially connected in a direction away from the first film 2. The glue layer is bonded to the first film 2, so as to connect the second film 3 and the first film 2. The air outlet channel 7 is arranged on the glue layer.

[0029] Referring to Figure 2 and Figure 3The thickness of the adhesive layer is less than 0.2 mm, and the thickness of the film layer is 0.05-0.15 mm. Specifically, the thickness of the adhesive layer is 0.15 mm, and the thickness of the film layer is 0.1 mm. Since the adhesive layer is very thin, it can effectively prevent bacteria, dust and other particles in the external atmosphere from entering the disc body 1, thereby ensuring the accuracy of the test.

[0030] With reference to Figure 2 Specifically, the film layer is a polycarbonate film or a polypropylene film, i.e., the film layer is made of a PC film or a PT film; the adhesive layer is double-sided adhesive tape, and the film layer is attached to the first film 2 by the double-sided adhesive tape. The double-sided adhesive tape is removed at the position corresponding to the through hole 6, so as to form an air outlet channel 7 between the film layer and the first film 2. The double-sided adhesive tape not only has low cost, is easy to obtain and convenient to use, but also can support the film layer. By removing the double-sided adhesive tape at the position corresponding to the through hole 6, the air outlet channel 7 is formed conveniently, and the discharge of gas is ensured. In addition, the double-sided adhesive tape has a small thickness, which can effectively prevent bacteria, dust and other particles in the external atmosphere from entering the disc body 1.

[0031] With reference to Figure 2 The second film 3 is offset from other through holes on the first film 2 or has a notch to avoid interference, so as to facilitate the normal use of the reagent disc.

[0032] With reference to Figure 2 and Figure 3 The depth of the blind hole 4 is equal to the depth of the air outlet channel 5 on the disc body 1. The depth of the blind hole 4 and the depth of the air outlet channel 5 refer to the length of the blind hole 4 and the air outlet channel 5 in the thickness direction of the disc body 1. When the gas enters the blind hole 4 from the air outlet channel 5, it can be quickly discharged from the blind hole 4.

[0033] With reference to Figure 1 The first film 2 is a transparent polycarbonate film, i.e., a PC film. The use of the high-transparency PC film has good dimensional stability and dimensional accuracy, so as to accurately cover the disc body 1 with the first film 2 during use, and align the through hole 6 with the corresponding blind hole 4.

[0034] With reference to Figure 1 and Figure 2 Further, the first film 2 is attached to the disc body 1 by an adhesive or double-sided adhesive tape. The attachment between the first film 2 and the disc body 1 helps to improve the sealing between the first film 2 and the disc body 1, and ensures that each open cavity or channel on the disc body 1 forms a closed structure.

[0035] Since the cavities and channels on the disc body 1 are processed by microfabrication technology, and the biochemical analyzer combines microfluidic technology, high-throughput biochemical analysis of a small amount of sample can be realized.

[0036] The implementation principle of the embodiment of the present application is that when in use, the customized first adhesive film 2 is attached to the surface of the disc body 1 by an adhesive or double-sided tape, the through hole 6 on the first adhesive film 2 is aligned with the corresponding blind hole 4, the through hole 6 and the corresponding blind hole 4 are communicated, then the second adhesive film 3 is attached to the surface of the first adhesive film 2 by an adhesive layer, one end of the gas outlet channel 7 is communicated with the corresponding through hole 6, and the other end is communicated with the atmosphere.

[0037] During the detection process, under the centrifugal action, the reagent in the annular channel enters the reaction chamber, the reagent fills the reaction chamber, and the gas in the reaction chamber is discharged to the exhaust channel 5 and the blind hole 4, then the gas enters the gas outlet channel 7 through the corresponding through hole 6, and finally is discharged to the atmosphere from the gas outlet channel 7. The original molecular air-permeable plug is replaced by a physical channel in the present application, so that the operator does not need to install multiple molecular air-permeable plugs on the disc body 1 before testing, not only saving manpower, but also shortening the preparation time before testing, and effectively improving the testing efficiency.

[0038] The above are preferred embodiments of the present application, and are not intended to limit the protection scope of the present application, therefore: any equivalent changes made according to the structure, shape, principle of the present application should be covered within the protection scope of the present application.

Claims

1. A ventilation structure of a microfluidic biochemical reagent disc, characterized in that: The application relates to a disc body (1), a first adhesive film (2) and a second adhesive film (3), a plurality of blind holes (4) are formed in the disc body (1), the blind holes (4) correspond to exhaust passages (5) on the disc body (1) one by one, the blind holes (4) are located at one end of the corresponding exhaust passages (5) far from a reaction chamber and are communicated with the corresponding exhaust passages (5), the first adhesive film (2) is covered on the disc body (1) and is used for sealingly forming a closed structure of each cavity or passage on the disc body (1), a plurality of through holes (6) are formed in the first adhesive film (2) and correspond to the blind holes (4) on the disc body (1) one by one, the through holes (6) are communicated with the corresponding blind holes (4), the second adhesive film (3) is arranged on the first adhesive film (2) and covers the plurality of through holes (6) on the first adhesive film (2), a plurality of air outlet passages (7) are formed in one side of the second adhesive film (3) close to the first adhesive film (2), the air outlet passages (7) correspond to the through holes (6) one by one, one end of the air outlet passages (7) is communicated with the corresponding through holes (6), and the other end is communicated with the atmosphere.

2. The microfluidic biochemical reagent disc ventilation structure according to claim 1, characterized in that: The second adhesive film (3) comprises an adhesive film layer and a glue layer, the glue layer and the adhesive film layer are sequentially arranged in a direction away from the first adhesive film (2), the glue layer is bonded with the first adhesive film (2), and the air outlet passages (7) are formed in the glue layer.

3. The microfluidic biochemical reagent disc ventilation structure according to claim 2, characterized in that: The thickness of the glue layer is less than 0.2 mm, and the thickness of the adhesive film layer is 0.05-0.15 mm.

4. The microfluidic biochemical reagent disc ventilation structure according to claim 2, characterized in that: The glue layer is double-sided adhesive.

5. The microfluidic biochemical reagent disc ventilation structure according to claim 2, characterized in that: The adhesive film layer and the glue layer are both circular arc-shaped, and the adhesive film layer, the glue layer and the disc body (1) are concentrically arranged.

6. The microfluidic biochemical reagent disc ventilation structure according to claim 1, characterized in that: The depth of the blind hole (4) is equal to the depth of the exhaust passage (5) on the disc body (1).

7. The microfluidic biochemical reagent disc ventilation structure according to claim 1, characterized in that: The through hole (6) is concentrically arranged with the corresponding blind hole (4), and the diameter of the through hole (6) is greater than that of the corresponding blind hole (4).

8. The microfluidic biochemical reagent disc ventilation structure according to claim 1, characterized in that: The first adhesive film (2) is a transparent polycarbonate film.

9. The microfluidic biochemical reagent disc ventilation structure according to claim 1, characterized in that: The first adhesive film (2) is bonded with the disc body (1).

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