Multi-row micro-fluidic chip monomer and chip group for nucleic acid extraction

Abstract

The utility model belongs to the field of micro-fluidic chips, and particularly relates to a multi-row micro-fluidic chip monomer and a chip group for nucleic acid extraction. The micro-fluidic chip monomer comprises a reaction carrier and a plurality of three-way valves integrated on the reaction carrier, and is characterized in that each three-way valve is provided with a first opening, a second opening and a third opening, each air hole in the reaction carrier is connected with the second opening of one three-way valve, and the first opening and the third opening of each three-way valve are located on the same straight line. The first and third opening directions of the three-way valves are vertical to the plane of the reaction carrier, and the three-way valves are arranged in parallel; connection matching structures are arranged on the reaction carriers and are used for connecting the reaction carriers in a laminated manner; the first opening and the third opening of the three-way valve are provided with matched connection structures. All the monomers are sequentially connected through a connection matching structure arranged on the reaction carrier and matching connection structures of the first opening and the third opening of the three-way valve at the air holes in the same position to form a chip group. According to the multi-row micro-fluidic chip provided by the utility model, a plurality of micro-fluidic chip monomers can be freely assembled according to actual working requirements of various working scenes and the number of samples, so that the simultaneous processing of a plurality of samples is realized.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of micro -fluidic chip, concretely relates to a kind of for nucleic acid extraction multi -row micro -fluidic chip monomer and chip group. BACKGROUND

[0002] Miniaturized micro -fluidic chip nucleic acid extraction technology can be complex nucleic acid extraction process concentrated on a chip, has become one of increasingly important tools in the field of nucleic acid extraction.

[0003] The micro -fluidic nucleic acid extraction chip equipment currently used is directly connected to the device with single micro -fluidic chip, and only one micro -fluidic chip (one sample) can be processed each time, so the sample processing efficiency is low, and it is difficult to meet the actual demand of simultaneous detection of multiple samples. SUMMARY

[0004] To solve the deficiencies of the prior art, the utility model provides a kind of multi -row micro -fluidic chip monomer and chip group, can handle multiple samples after sample addition simultaneously after being connected to equipment.

[0005] To achieve the above purpose, the technical scheme adopted by the utility model is:

[0006] A kind of multi -row micro -fluidic chip monomer for nucleic acid extraction, including reaction carrier and several three-way valves integrated on reaction carrier, the three-way valve has first, second, third opening, every gas hole on reaction carrier is connected with the second opening of a three-way valve, the first, third opening of three-way valve is on the same straight line, the direction of the first, third opening of three-way valve is perpendicular to the plane of reaction carrier, and each three-way valve is arranged in parallel;Reaction carrier is provided with connection matching structure for laminating connection of reaction carrier;The first, third opening of the three-way valve has matching connection structure.

[0007] Precise micro valve is arranged in the gas hole, and the flow direction of gas and liquid in the chip channel is realized by micro valve and micro pump of host computer.

[0008] Other settings on the reaction carrier can be designed regularly according to the need of extraction process, for example, a plurality of independent liquid storage cavities, sample addition cavities, main reaction cavities, a plurality of gas holes, waste liquid cavities and storage cavities are provided;Each independent liquid storage cavity, sample addition cavity, main reaction cavity is communicated with 1 gas hole by micro -flow channel, and a plurality of micro -liquid path channels are integrated on the reaction carrier, and the main reaction cavity is communicated with each independent liquid storage cavity, sample addition cavity, waste liquid cavity and storage cavity by micro -liquid path channel.

[0009] More specifically, taking the magnetic bead method as an example, the liquid storage cavity is 5, which is lysis liquid cavity, magnetic bead suspension liquid cavity, washing liquid cavity, eluent cavity and buffer cavity;Then the gas hole is 7.

[0010] On the basis, the utility model further provides a kind of for nucleic acid extraction multi-arrangement microfluidic chip group, the chip group includes multiple above-mentioned multi-arrangement microfluidic chip monomer, each monomer is sequentially connected by the connecting cooperation structure being provided on reaction carrier and the cooperation connection structure of the first, third opening of three-way valve at the same position air hole, to form the chip group with multilayer reaction carrier, and the same position three-way valve on each reaction carrier is sequentially connected to form gas passage.

[0011] When using, after microfluidic chip is added sample and is placed into microfluidic chip extraction equipment, the gas source channel of equipment is connected with chip air hole by three-way valve, the connection channel between liquid storage cavity and air hole, liquid storage cavity and reaction pool, liquid adding cavity and air hole and liquid adding cavity and reaction pool, equipment can realize the liquid path flow and control between liquid storage cavity and reaction pool, liquid adding cavity and reaction pool, magnetic bead cavity and reaction pool, reaction pool and waste liquid cavity, reaction pool and storage cavity by the micro-control of the air pressure of each air hole by differential pressure, to realize the processes such as cracking, washing, elution, purification in nucleic acid extraction process, produce high-purity nucleic acid end product, temporarily store in the liquid storage cavity of microfluidic chip, wait for transfer or detection.

[0012] Precise microvalve is built in each air hole, realizes liquid driving by positive pressure and negative pressure, is used to control the accurate flow of airflow, simultaneously prevents gas from crossing flow between different chips.

[0013] The connecting cooperation structure on the reaction carrier of a specific microfluidic chip monomer can adopt fixed buckle form: for example, there are four buckles for fixing relative position on four corners of each microfluidic chip, when several chips need to be assembled, accurate positioning is realized by corresponding buckle on four corners of each microfluidic chip, and mutual buckling forms a parallel whole.

[0014] Three-way valve in the utility model is the key of the technology.When monomer microfluidic chip is connected with equipment, the gas source channel of equipment is connected with chip air hole by improved three-way valve, the valve in the direction of the first second two openings participating in connection is automatically opened, and the third opening direction not participating in connection is automatically closed.Further, sealing facility is arranged on three-way valve.

[0015] When the equipment of microfluidic nucleic acid extraction chip needs to process multiple samples at a time, after each sample is added to a monomer microfluidic chip, multiple microfluidic chip monomers are connected by three-way valve, all participating connection holes are automatically opened, and the third opening hole not participating in connection of the last microfluidic chip is automatically closed.All participating reaction chips are successfully connected in parallel on microfluidic chip extraction equipment by three-way valve, to realize the communication with equipment gas source.The sealing facility integrated on three-way valve can be automatically sealed.

[0016] Compared with the prior art, the utility model has the advantages that the multi-row micro-fluidic chip provided by the utility model can be freely assembled according to the actual work needs of various work scenes and the number of samples, so that the simultaneous processing of multiple samples is realized. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is a micro-fluidic chip monomer schematic diagram of the utility model.

[0018] Figure 2 It is a reaction carrier plane schematic diagram of the micro-fluidic chip monomer.

[0019] Figure 3 It is a single three-way valve working schematic diagram.

[0020] Figure 4 It is a multiple three-way valve connection schematic diagram.

[0021] 1: sample adding cavity, 2: air hole, 3: lysis liquid cavity, 4: magnetic bead suspension cavity, 5: washing liquid cavity, 6: eluent cavity, 7: buffer cavity, 8: fixed buckle, 9: main reaction cavity, 10: waste liquid cavity, 11: storage cavity, 21: first opening, 22: second opening, 23: third opening, 100: reaction carrier, 200: three-way valve. DETAILED DESCRIPTION

[0022] In order to better understand the technical scheme of the utility model, the following will be specifically described in conjunction with the drawings, but should not be understood as limiting the protection scope of the utility model.

[0023] Example one

[0024] A kind of multi-row micro-fluidic chip monomer for nucleic acid extraction, including reaction carrier and several three-way valves integrated on reaction carrier, the three-way valve has first, second, third opening, every air hole on reaction carrier is connected with the second opening of a three-way valve, the first, third opening of three-way valve is on same straight line, the first, third opening direction of three-way valve is perpendicular to reaction carrier plane, each three-way valve is parallel arrangement;Reaction carrier is provided with connection matching structure, for reaction carrier lamination connection;The first, third opening of the three-way valve has matching connection structure;The micro valve is arranged in the air hole.The following is described in conjunction with the drawings.

[0025] The multi-row micro-fluidic chip monomer schematic diagram is as Figure 1, including a reaction carrier 100 and seven micro three-way valves 200 installed on the outside of seven air holes of the reaction carrier, and the three-way valves are arranged in parallel; the length of the three-way valves is basically the same as the thickness of the reaction carrier; the reaction carrier is provided with a connecting and matching structure for connecting, fixing and positioning the reaction carrier in layers, and the three-way valves on each air hole are also connected to each other through the self-provided bayonet. Figure 2 , taking five liquid storage cavities as an example, the reaction carrier 100 has five liquid storage cavities (lysis liquid cavity 3, magnetic bead suspension liquid cavity 4, washing liquid cavity 5, elution liquid cavity 6 and buffer liquid cavity 7), a sample adding cavity 1, a main reaction cavity 9, seven air holes 2, a waste liquid cavity 10 and a storage cavity 11; the air holes 2 are provided with micro valves, and the seven air holes 2 are respectively communicated with the lysis liquid cavity 3, the magnetic bead suspension liquid cavity 4, the washing liquid cavity 5, the elution liquid cavity 6, the buffer liquid cavity 7, the sample adding cavity 1 and the main reaction cavity 9 through micro flow channels; a plurality of micro liquid path channels are integrated inside the micro fluidic chip, and the main reaction cavity 9 is communicated with the liquid storage cavities (lysis liquid cavity 3, magnetic bead suspension liquid cavity 4, washing liquid cavity 5, elution liquid cavity 6 and buffer liquid cavity 7), the sample adding cavity 1, the waste liquid cavity 10 and the storage cavity 11 through the micro liquid path channels. The blue line is the air passage, and the green line is the liquid passage. The collected sample is added to the sample adding cavity 1, the sample adding hole is provided with a structure for preventing sample backflow, and the cover is closed after sample adding; there are seven air holes 2, each air hole is provided with a precise micro valve for controlling the accurate flow of air flow and switching positive pressure and negative pressure to realize liquid driving; after sample adding, the lysis liquid in the lysis liquid cavity 3 is pushed by air pressure and injected into the sample adding cavity 1; there are four fixing buckles 8 on four corners of each micro fluidic chip monomer for fixing the relative position, and when several chip monomers need to be assembled, precise positioning is realized through the corresponding buckles on the four corners of each micro fluidic chip, and the buckles are tightly buckled to each other to form a parallel whole.

[0026] Figure 3 is a single three-way valve schematic diagram, the three-way valve 200 has a first opening 21, a second opening 22 and a third opening 23, the first opening 21 is an air passage inlet, and the third opening 23 is a matching connection structure, the device gas source interface structure is the same as the third opening 23, which can smoothly realize the matching connection with the first opening 21 and the third opening 23, and in operation, the three-way valve is connected with the device gas source through the first opening 21, and the gas flows into the three-way valve from the first opening 21; the second opening 22 of the three-way valve is an air passage outlet, which is communicated with the air hole of the micro fluidic chip, and the gas flows into the micro fluidic chip through the second opening 22 and controls the liquid flow direction in the chip. The arrow indicates the flow direction of the working air flow of the single micro fluidic chip. The third opening 23 of the three-way valve is also a gas passage, and when the single micro fluidic chip is used for nucleic acid extraction, the third opening 23 is automatically closed.

[0027] Figure 4The connection diagram of multiple three-way valves is shown, and three three-way valves 200 are taken as an example. The three-way valves are connected to each other through the matching connection structure of the first opening 21 and the third opening 23. The first opening 21 of the first three-way valve of each group of chip array is connected to the gas source of the equipment. The second opening 22 of the three-way valve is the gas passage outlet, which is connected to the internal gas passage of the microfluidic chip unit. The third opening 23 of the three-way valve is the gas passage outlet. When the microfluidic chip is assembled, the third opening 23 of the previous three-way valve is connected to the first opening 21 of the next three-way valve. After that, the gas passage outlets of the third openings 23 of the first to the second-to-last three-way valves are automatically opened, while the third opening 23 of the three-way valve of the last microfluidic chip unit remains closed, realizing the connection of the group of chip array to the gas source of the equipment through the improved three-way valves. Figure 4 The gas flow direction is shown by arrows.

[0028] A microfluidic chip group is taken as an example, and each microfluidic chip unit has seven gas holes, so seven micro three-way valves are integrated. When the chip is assembled, the reaction carriers 100 of the three microfluidic chip units are connected in layers through the fixing buckle 8. The three-way valves at the same position on each reaction carrier are connected in turn through the first opening and the third opening, forming seven groups of three-way valve arrays. The first opening of the first three-way valve of each group of three-way valves is directly connected to the gas passage of the host.

Claims

1. A multi-row microfluidic chip monomer for nucleic acid extraction, comprising a reaction carrier and a plurality of three-way valves integrated on the reaction carrier, characterized in that, The three-way valve has first, second and third openings, each gas hole on the reaction carrier is connected with the second opening of one three-way valve, the first and third openings of the three-way valve are on the same straight line, the directions of the first and third openings of the three-way valve are perpendicular to the plane of the reaction carrier, and the three-way valves are arranged in parallel; the reaction carrier is provided with a connecting and matching structure for connecting and stacking the reaction carriers; the first and third openings of the three-way valve have a matching connecting structure.

2. The multi-row microfluidic chip monomer for nucleic acid extraction according to claim 1, characterized in that, The gas hole is provided with a precision micro valve, and the micro valve and a micro pump of a host computer are used to realize accurate control of the flow direction of gas and liquid in the chip channel. 3.The multi-connection micro-fluidic chip monomer for nucleic acid extraction of claim 2, wherein, The reaction carrier is provided with a plurality of independent liquid storage cavities, sample adding cavities, main reaction cavities, a plurality of gas holes, waste liquid cavities and storage cavities; the independent liquid storage cavities, the sample adding cavities and the main reaction cavities are respectively connected with one gas hole through micro flow channels; the reaction carrier is integrated with a plurality of micro liquid path channels; and the main reaction cavities are respectively connected with the independent liquid storage cavities, the sample adding cavities, the waste liquid cavities and the storage cavities through the micro liquid path channels.

4. The multi-row microfluidic chip monomer for nucleic acid extraction according to claim 3, characterized in that, The reaction carrier is provided with five independent liquid storage cavities, namely, a lysis liquid cavity, a magnetic bead suspension liquid cavity, a washing liquid cavity, an elution liquid cavity and a buffer liquid cavity; and the gas hole is seven.

5. A multi-row microfluidic chip set for nucleic acid extraction, characterized by, A plurality of the microfluidic chip monomers for nucleic acid extraction according to claim 1 are connected in series through the connecting and matching structure on the reaction carrier and the matching connecting structure of the first and third openings of the three-way valves at the same position gas hole.

6. The multi-cascade microfluidic chip set for nucleic acid extraction according to claim 5, wherein, The gas hole is provided with a micro valve.

7. The multi-cascade microfluidic chip set for nucleic acid extraction according to claim 6, wherein, The reaction carrier is provided with a plurality of independent liquid storage cavities, sample adding cavities, main reaction cavities, a plurality of gas holes, waste liquid cavities and storage cavities; the independent liquid storage cavities, the sample adding cavities and the main reaction cavities are respectively connected with one gas hole through micro flow channels; the reaction carrier is integrated with a plurality of micro liquid path channels; and the main reaction cavities are respectively connected with the independent liquid storage cavities, the sample adding cavities, the waste liquid cavities and the storage cavities through the micro liquid path channels.

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