Experimental device suitable for water layer distribution of zooplankton in water body

Abstract

The utility model discloses an experimental device suitable for water layer distribution of zooplankton in a water body, which comprises an upper-layer water tank, a middle-layer water tank and a lower-layer water tank which are respectively provided with a water outlet valve; the upper-layer water tank and the middle-layer water tank are connected through one butterfly valve and one plastic hoop, the middle-layer water tank and the lower-layer water tank are also connected through one butterfly valve and one plastic hoop, the plastic hoops are arranged on the outer sides of the butterfly valves in a sleeving mode and used for reinforcing the bottoms of the water tanks on the corresponding layers so as to prevent the water tanks from cracking, and the butterfly valves achieve the purpose of separating different water layers; the experimental device can simulate water body parameters according to different water body requirements so as to explore the water layer distribution condition of zooplankton, and can better control environmental variables in the water body so as to explore the influence of a single factor on the zooplankton distribution condition, thereby mastering the distribution rule so as to formulate an ecological prevention and control strategy for harmful zooplankton.

Description

TECHNICAL FIELD

[0001] The utility model belongs to ecological environment technical field, especially suitable for water body plankton water layer distribution's experimental apparatus. BACKGROUND

[0002] Zooplankton is a kind of heterotrophic organism that floats or weakly moves in water, usually small invertebrates or the larva of chordates. Zooplankton is diverse, from unicellular protozoa to multicellular crustaceans and cnidarians, covering almost all major animal phyla, mainly including protozoa, rotifers, cladocerans and copepods. Zooplankton cannot autonomously manufacture organic matter and mainly feeds on phytoplankton, bacteria and organic detritus, playing an important role in aquatic ecosystems. They are not only an important bait for fish and other aquatic animals, but also an indicator organism for water quality and ecological environment, and a bridge connecting producers and higher trophic levels.

[0003] Nowadays, there are various means for collecting and quantifying zooplankton, mainly including plankton nets and water sampling and filtering. Although the above sampling surveys can generally reflect the quantity and distribution of various or specific zooplankton in water layers, they are limited by various conditions such as sampling time, dissolved oxygen content, temperature, light and other conditions, resulting in large differences in the distribution of zooplankton in different water layers or regions of the same water body, and the distribution of sample quantity cannot well represent the water layer preference of zooplankton under the same conditions. Or when zooplankton aggregates in the water body, the success of sampling survey depends largely on the selection of sampling points and the operation of the collection process. The method of using plankton nets to collect zooplankton quantitatively is prone to cause water layer disturbance and is not suitable for water layer distribution investigation. Therefore, from the perspective of establishing a water layer distribution model, a simulated water layer that can control a single or specific environmental factor can be designed to eliminate sampling errors and explore the distribution device of zooplankton. UTILITY MODEL CONTENT

[0004] The utility model provides a kind of experimental device suitable for water body plankton water layer distribution, which can simulate water body parameters according to different water body requirements to explore the water layer distribution of zooplankton, and can better control the environmental variables in water body to explore the influence of single factor on the distribution of zooplankton, so as to accurately grasp its distribution rule, which is also conducive to formulating ecological control strategy for harmful plankton.

[0005] To solve the above problems, the technical scheme provided by the utility model is as follows:

[0006] This utility model embodiment provides an experimental device suitable for the water layer distribution of zooplankton in aquatic bodies, including an upper water tank (1-1), a middle water tank (1-2) and a lower water tank (1-3), wherein each of the upper water tank (1-1), the middle water tank (1-2) and the lower water tank (1-3) is provided with a water outlet valve (2);

[0007] The upper water tank (1-1) and the middle water tank (1-2) are connected by a butterfly valve (3) and a plastic clamp (4). The middle water tank (1-2) and the lower water tank (1-3) are also connected by a butterfly valve (3) and a plastic clamp (4). The plastic clamp (4) is fitted on the outside of the butterfly valve (3) to reinforce the bottom of the corresponding water tank to prevent it from cracking. The butterfly valve (3) is used to separate different water layers.

[0008] The height of the upper water tank (1-1), the middle water tank (1-2) and the lower water tank (1-3) is 500mm. The height of the circular outer shell (3-3) of the butterfly valve (3) is 160mm. The inner diameter of the circular outer shell (3-3) is 200mm. The outlet valve (2) is located 100mm above the adjacent butterfly valve (3). The height of the plastic hoop (4) is 50mm.

[0009] In an optional embodiment of this utility model, the butterfly valve (3) includes a handle (3-1), a fixing plate (3-2), a circular outer shell (3-3), a rotating shaft (3-4), and a circular butterfly plate (3-5); the rotating shaft (3-4) and the circular butterfly plate (3-5) are connected, and the rotating shaft (3-4) is also connected to the handle (3-1); one side of the fixing plate (3-2) is connected to the handle (3-1), and the other side is connected to the circular outer shell (3-3); the circular butterfly plate (3-5) is located inside the cavity of the circular outer shell (3-3).

[0010] In an optional embodiment of this utility model, a 20mm diameter air hole (5) is provided below the water outlet valve (2) of the middle layer water tank (1-2) and the lower layer water tank (1-3) to balance the pressure inside and outside the tank body, so that the water in the lower layer water tank (1-3) can flow out smoothly. The air hole (5) can be filled with a rubber stopper or a one-way air valve for pressure control.

[0011] In one optional embodiment of this utility model, the upper water tank (1-1), the middle water tank (1-2), and the lower water tank (1-3) are all acrylic transparent pipes.

[0012] Compared with the prior art, the present invention provides an experimental device suitable for the water layer distribution of zooplankton in aquatic bodies, which has the following beneficial effects: (1) The experimental device can simulate the vertical distribution of zooplankton in natural water layers, prevent sampling errors caused by sampling location and human factors, and is more objective than traditional sampling surveys. (2) The experimental device uses stratified water outlets, and the addition of butterfly valves between each water layer can effectively prevent water flow disturbances in each water layer from causing errors. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0014] Figure 1 This is a schematic diagram of the structural composition of an experimental apparatus suitable for the water layer distribution of zooplankton in water, provided in an embodiment of this application.

[0015] Figure 2 A front view of a butterfly valve in an experimental apparatus for the distribution of zooplankton in water, provided in an embodiment of this application.

[0016] Figure 3 A side view of a butterfly valve in an experimental apparatus for the distribution of zooplankton in water, provided in an embodiment of this application.

[0017] Figure 4 This is a physical diagram of an experimental apparatus suitable for the water layer distribution of zooplankton in water, provided as an embodiment of this application.

[0018] Figure 5 This is a schematic diagram showing the relationship between the DNA copy number of Ichthyophthirius multifiliis and the water layer distribution in an experimental apparatus suitable for the distribution of zooplankton in aquatic layers, provided in an embodiment of this application. Detailed Implementation

[0019] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application.

[0020] like Figure 1 , Figure 2 , Figure 3 and Figure 4As shown, this utility model embodiment provides an experimental device suitable for the water layer distribution of zooplankton in water bodies, including an upper water tank 1-1, a middle water tank 1-2 and a lower water tank 1-3. The upper water tank 1-1, the middle water tank 1-2 and the lower water tank 1-3 are all acrylic transparent pipes, and the three acrylic transparent pipes form a self-made layered water tank.

[0021] Each of the upper water tank 1-1, the middle water tank 1-2, and the lower water tank 1-3 is equipped with a water outlet valve 2. The upper water tank 1-1 and the middle water tank 1-2 are connected by a butterfly valve 3 and a plastic clamp 4, and the middle water tank 1-2 and the lower water tank 1-3 are also connected by a butterfly valve 3 and a plastic clamp 4. The plastic clamp 4 is fitted over the butterfly valve 3 to reinforce the bottom of the corresponding water tank layer and prevent cracking. The butterfly valve 3 is used to separate different water layers. For example, the plastic clamp 4 between the upper water tank 1-1 and the middle water tank 1-2 prevents the bottom of the upper water tank 1-1 from cracking, and the plastic clamp 4 between the middle water tank 1-2 and the lower water tank 1-3 prevents the bottom of the middle water tank 1-2 from cracking.

[0022] The upper water tank 1-1, middle water tank 1-2, and lower water tank 1-3 are all 500mm high. The circular outer shell 3-3 of the butterfly valve 3 is 160mm high, and the inner diameter of the circular outer shell 3-3 is 200mm. The outlet valve 2 is located 100mm above the adjacent butterfly valve 3. The plastic clamp 4 is 50mm high. In this embodiment, the three 500mm acrylic transparent pipes are connected by two 200mm diameter butterfly valves and assembled into a whole water tank with a total length of 1500mm. The length of the three acrylic transparent pipes can be adjusted according to the actual depth of the natural water body.

[0023] A 20mm diameter air hole 5 is provided below the water outlet valve 2 of the middle water tank 1-2 and the lower water tank 1-3 to balance the pressure inside and outside the tank and allow water to flow out of the lower water tank 1-3 smoothly. The air hole 5 can be filled with a rubber plug or a one-way air valve for pressure control.

[0024] like Figure 2 and Figure 3 As shown, the butterfly valve 3 includes a handle 3-1, a fixing plate 3-2, a circular outer shell 3-3, a rotating shaft 3-4, and a circular butterfly plate 3-5. The rotating shaft 3-4 is connected to the circular butterfly plate 3-5, and the rotating shaft 3-4 is also connected to the handle 3-1. One side of the fixing plate 3-2 is connected to the handle 3-1, and the other side is connected to the circular outer shell 3-3. The circular butterfly plate 3-5 is located inside the cavity of the circular outer shell 3-3. In this embodiment, the butterfly valve 3 can control the opening and closing degree of the circular butterfly plate 3-5 by rotating the external handle 3-1, thereby achieving the purpose of separating different water layers.

[0025] Taking the ciliated larval stage of Ichthyophthirius multifiliis (predatory stage) as an example, this paper introduces the method of using an experimental apparatus suitable for the distribution of zooplankton in aquatic layers, including the following steps:

[0026] (1) Collection of zooplankton to be tested: Ichthyophthirius multifiliis cysts were collected in 50ml petri dishes and incubated at 25℃. After 16h, the predators were collected after hatching from the cysts, and the insect density was calculated.

[0027] (2) Adjust all butterfly valves to the open position, fill half a tank of water (about 24L), and then inject the concentrated solution of Ichthyophthirius multifiliis predator into the tank according to the experimental requirements (about 20,000 insects / tank). Then fill the tank with water and transfer it to a specific environment to ensure that the experimental conditions are consistent. Let it stand for 1, 2 and 3 hours respectively to allow the insects to be fully distributed in the tank.

[0028] (3) Sample collection: Slowly close the butterfly valves of each layer to reduce disturbance to the water layer. After each layer of water is released from the outlet, it is filtered through a 5μm pore size cellulose acetate membrane. Then, the DNA on the filter membrane is extracted and purified using the Water DNA Isolation Kit (Foregene, China). All DNA samples are stored in a -80℃ freezer for subsequent quantitative detection.

[0029] (4) qPCR quantification: DNA samples were amplified using specific primers and probes, and the amplification products were then introduced into the pESI-T vector. Plasmids were extracted using the SanPrep small volume plasmid extract kit and then linearized using HindIII restriction endonuclease. The plasmid DNA concentration was detected using the dsDNA high sensitivity assay kit with a Qubit 3.0 fluorometer. Finally, the linear plasmid DNA concentration was converted to copy number using the online tool DNA CopyNumber and DilutionCalculator.

[0030] (5) Analyze the data and count the differences in copy number between different water layers to estimate the number of Ichthyophthirius multifiliis predators distributed between each water layer.

[0031] like Figure 5 The results of the top-level illumination experiment showed that the gene abundance of Ichthyophthirius multifiliis was higher in the upper water layer at 1 hour and 2 hours, and the distribution of predators differed significantly between the treatment groups at different time points.

[0032] In summary, although the present invention has been disclosed above with reference to preferred embodiments, the above preferred embodiments are not intended to limit the present invention. Those skilled in the art can make various modifications and refinements without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention shall be determined by the scope defined in the claims.

Claims

1. An experimental apparatus suitable for the water layer distribution of zooplankton in aquatic bodies, characterized in that, It includes an upper water tank (1-1), a middle water tank (1-2) and a lower water tank (1-3), and each of the upper water tank (1-1), the middle water tank (1-2) and the lower water tank (1-3) is equipped with a water outlet valve (2); The upper water tank (1-1) and the middle water tank (1-2) are connected by a butterfly valve (3) and a plastic hoop (4). The middle water tank (1-2) and the lower water tank (1-3) are also connected by a butterfly valve (3) and a plastic hoop (4). The plastic hoop (4) is fitted on the outside of the butterfly valve (3) to reinforce the bottom of the corresponding water tank to prevent it from cracking. The butterfly valve (3) is used to separate different water layers; The height of the upper water tank (1-1), the middle water tank (1-2) and the lower water tank (1-3) is 500mm. The height of the circular outer shell (3-3) of the butterfly valve (3) is 160mm. The inner diameter of the circular outer shell (3-3) is 200mm. The outlet valve (2) is located 100mm above the adjacent butterfly valve (3). The height of the plastic hoop (4) is 50mm.

2. The experimental apparatus for the water layer distribution of zooplankton in water bodies according to claim 1, characterized in that, The butterfly valve (3) includes a handle (3-1), a fixing plate (3-2), a circular outer shell (3-3), a rotating shaft (3-4), and a circular butterfly plate (3-5); the rotating shaft (3-4) and the circular butterfly plate (3-5) are connected, and the rotating shaft (3-4) is also connected to the handle (3-1); one side of the fixing plate (3-2) is connected to the handle (3-1), and the other side is connected to the circular outer shell (3-3); the circular butterfly plate (3-5) is located inside the cavity of the circular outer shell (3-3).

3. The experimental apparatus for the water layer distribution of zooplankton in water bodies according to claim 1, characterized in that, The middle water tank (1-2) and the lower water tank (1-3) have 20mm diameter air holes (5) below their outlet valves (2) to balance the pressure inside and outside the tank and allow water to flow out of the lower water tank (1-3) smoothly. The air holes (5) can be filled with rubber plugs or one-way valves for pressure control.

4. The experimental apparatus for the water layer distribution of zooplankton in water bodies according to claim 1, characterized in that, The upper water tank (1-1), the middle water tank (1-2), and the lower water tank (1-3) are all transparent acrylic pipes.

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