Plant pathogen pathogenic gene target screening device
By designing a fixation and positioning system for the conical flask, the problem of displacement and tipping of the flask during shaker oscillation was solved, thus achieving stability in microbial culture and accuracy in detection results, and providing a stable experimental model.
Patent Information
- Application Number
- CN202522326239.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-03
- Publication Date
- 2025-12-09
- Estimated Expiration
- 2035-11-03
AI Technical Summary
In traditional experiments, conical flasks are prone to displacement or tipping over during high-speed shaking in a shaker, affecting the accuracy of experimental results and the uniformity of the microbial culture medium.
A device for screening pathogenic gene targets of plant pathogens was designed. The device uses a first fixing component and a second fixing component to clamp and fix the conical flask to prevent it from shifting or tipping over during shaking. The device also uses a positioning component to ensure stable position, thereby achieving physical isolation of microorganisms and directional delivery of active substances.
This effectively avoids shaking of the conical flask and spillage of the culture medium, ensuring the stability of microbial culture and the accuracy of subsequent gene expression detection, and providing a stable and controllable experimental model.
Smart Images
Figure CN223646540U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of physical pathology technology, specifically to a device for screening pathogenic gene targets of plant pathogens. Background Technology
[0002] With the development of molecular biology techniques, screening pathogenic gene targets and developing targeted disease control measures, such as gene silencing control strategies based on RNA interference technology, has become one of the core directions of plant disease control research. In the screening of pathogenic gene target segments of plant pathogens, dsRNA expressed by biocontrol microorganisms such as Trichoderma harzianum can be used to target and regulate pathogenic gene targets. By detecting changes in the expression level of target genes, it can be verified whether the target segment has the potential to control the disease. The key to this research process is to establish a stable experimental system to ensure that the supernatant of biocontrol microorganisms can be continuously injected into the culture medium containing pathogen spores, while ensuring that the two microorganisms do not interfere with each other during the shaking incubation process, and that the stability of the experimental device does not affect the sample processing effect and the accuracy of the detection results.
[0003] Traditional experiments often use simple shakers with conical flasks for sample culture, but lack a dedicated fixing structure for the conical flasks. During the high-speed shaking of the shaker, the conical flasks are prone to displacement, tipping, or violent shaking, which can lead to spillage of the culture medium, damage the growth environment of microorganisms, affect the uniformity of mixing of the supernatant and pathogenic spores, and thus interfere with the subsequent gene expression detection results. Therefore, the artisans in this field provide a device for screening plant pathogenic gene targets to solve the problems mentioned in the background art. Utility Model Content
[0004] The purpose of this invention is to provide a device for screening plant pathogenic gene targets, which solves the problem in the prior art that the conical flask is prone to displacement or even tipping over during the high-speed shaking of the shaker, thus affecting the accuracy of the test results.
[0005] This utility model provides the following technical solution: a plant pathogenic gene target screening device, including a screening component, a first fixing component for preventing the screening component from shaking is provided on the upper side of the screening component, a second fixing component for locking the first fixing component is provided on both sides of the first fixing component, and a positioning component for fixing the position of the opened first fixing component is provided inside the first fixing component; the first fixing component includes two positioning blocks, and a placement groove is opened on the adjacent side of the two positioning blocks, and a rubber pad is fixedly connected to the inner wall of the placement groove.
[0006] As a preferred embodiment of the above technical solution, the screening component includes a workbench, a shaker is placed on the upper part of the workbench, a fixed platform is fixedly connected to the upper part of the shaker, two conical flasks are placed on the upper part of the fixed platform, and connecting pipes are fixedly connected to the lower ends of the two conical flasks. A peristaltic pump is placed on the upper part of the workbench on one side of the shaker, and the ends of the two connecting pipes are fixedly connected to the peristaltic pump. A filter is fixedly connected to the connecting pipe.
[0007] As a preferred embodiment of the above technical solution, the first fixing component includes two positioning blocks. Each of the two positioning blocks has a placement groove adapted to the conical bottle on its adjacent side. A rubber pad is fixedly connected to the inner wall of the placement groove. A guide groove is provided at the upper end of the fixing platform. A guide block is slidably fitted inside the guide groove. The guide block is fixedly connected to the lower end of the positioning block.
[0008] As a preferred embodiment of the above technical solution, the second fixing component includes a first fixing block and a second fixing block. The first fixing block and the second fixing block are respectively fixedly connected to two positioning blocks. A rotating rod is rotatably sleeved in the middle of the second fixing block. A fixing disk is fixedly connected to the end of the rotating rod away from the first fixing block. An opening is provided in the middle of the first fixing block. A first spring is sleeved on the outside of the rotating rod between the second fixing block and the fixing disk. A slot is provided on the side of the first fixing block away from the second fixing block. A locking block is movably engaged inside the slot. The locking block is fixedly connected to the rotating rod.
[0009] As a preferred embodiment of the above technical solution, the positioning component includes a positioning insert plate, an inner groove is formed inside the positioning block, the positioning insert plate is movably fitted inside the inner groove, a slot is formed at the upper end of the fixing platform, the end of the positioning insert plate is movably inserted into the slot, a groove is formed on one side of the inner groove, a sliding block is movably fitted inside the groove, and the sliding block is fixedly connected to the positioning insert plate.
[0010] As a preferred embodiment of the above technical solution, a first connecting rod is fixedly connected to the inner wall of the groove, a second connecting rod is movably sleeved inside the first connecting rod, the lower end of the second connecting rod extends out of the first connecting rod and is fixedly connected to the upper end of the sliding block, and a second spring located inside the first connecting rod is fixedly connected to the upper end of the second connecting rod.
[0011] Compared with the prior art, the beneficial effects of this utility model are:
[0012] This plant pathogenic gene target screening device uses a first and a second fixing component to clamp and fix the conical flask, thus fixing the relative position between the conical flask and the fixed platform. This prevents the conical flask from shifting, tipping, or violently shaking during shaking, which could lead to spillage of the culture medium. At the same time, it completely separates beneficial bacteria from pathogens in space, fundamentally avoiding bacterial contamination and creating a prerequisite for single-strain research. Furthermore, based on physical isolation, it enables the continuous and targeted delivery of active substances secreted by beneficial bacteria in the conical flask to the pathogen environment, achieving dynamic intervention and providing a stable and controllable dedicated model for experimental operations. Attached Figure Description
[0013] Figure 1 One of the three-dimensional structural schematic diagrams of a plant pathogen pathogenic gene target screening device;
[0014] Figure 2 This is the second three-dimensional structural schematic diagram of a plant pathogen pathogenic gene target screening device;
[0015] Figure 3 This is a schematic diagram of the screening component of a plant pathogen pathogenic gene target screening device.
[0016] Figure 4 A device for screening pathogenic gene targets of plant pathogens Figure 3 A magnified schematic diagram of the local structure at point A;
[0017] Figure 5 A schematic diagram of the first fixed component of a plant pathogen pathogenic gene target screening device;
[0018] Figure 6 A schematic diagram of the structure of the second fixed component of a plant pathogen pathogenic gene target screening device;
[0019] Figure 7 A schematic diagram of the positioning component of a plant pathogen pathogenic gene target screening device;
[0020] Figure 8 A device for screening pathogenic gene targets of plant pathogens Figure 7 A magnified view of the structure at point B in the middle;
[0021] Figure 9 This is a schematic diagram of the first and second connecting rods of a plant pathogen pathogenic gene target screening device.
[0022] In the diagram: 1. Screening component; 11. Workbench; 12. Shaker; 13. Fixed platform; 14. Conical flask; 15. Connecting pipe; 16. Peristaltic pump; 17. Filter; 2. First fixed component; 21. Positioning block; 22. Placement groove; 23. Rubber pad; 24. Guide groove; 25. Guide block; 3. Second fixed component; 31. First fixed block; 32. Second fixed block; 33. Rotating rod; 34. Fixed plate; 35. Through port; 36. First spring; 37. Slot; 38. Locking block; 4. Positioning component; 41. Positioning insert plate; 42. Inner groove; 43. Slot; 44. Sliding block; 45. First connecting rod; 46. Second connecting rod; 47. Second spring. Detailed Implementation
[0023] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention.
[0024] Please see Figures 1-9 As shown, this utility model provides a technical solution: a plant pathogenic gene target screening device, including a screening component 1, a first fixing component 2 for preventing the screening component 1 from shaking is provided on the upper side of the screening component 1, a second fixing component 3 for locking the first fixing component 2 is provided on both sides of the first fixing component 2, and a positioning component 4 for fixing the position of the opened first fixing component 2 is provided inside the first fixing component 2.
[0025] When the conical flask 14 is shaken, the position of the conical flask 14 is fixed by the first fixing component 2 to prevent the conical flask 14 from shaking. When the conical flask 14 is fixed by the first fixing component 2, the position of the first fixing component 2 is locked by the second fixing component 3 to increase the fixing effect. When the conical flask 14 is placed, the position of the opened first fixing component 2 is fixed by the positioning component 4 to facilitate user operation.
[0026] As one implementation method in this embodiment, please refer to Figures 1-3 As shown, the screening component 1 includes a workbench 11, a shaker 12 is placed on the upper end of the workbench 11, a fixed platform 13 is fixedly connected to the upper end of the shaker 12, two conical flasks 14 are placed on the upper end of the fixed platform 13, and connecting pipes 15 are fixedly connected to the lower ends of the two conical flasks 14. A peristaltic pump 16 is placed on the upper end of the workbench 11 and located on one side of the shaker 12. The ends of the two connecting pipes 15 are fixedly connected to the peristaltic pump 16, and filters 17 are fixedly connected to the connecting pipes 15.
[0027] During the operation, the staff first mixes the plant pathogen or biocontrol microorganism sample to be tested with the culture medium in a certain proportion, and then puts it into two conical flasks 14. The conical flasks 14 are then placed in the designated position on the fixed platform 13 in the screening component 1. At the same time, the connecting pipe 15 at the lower end of the conical flask 14 is connected to the peristaltic pump 16 on the workbench 11 to ensure that the subsequent liquid transfer channel is unobstructed. The two conical flasks 14 physically separate the two microorganisms to avoid bacterial mixing. Meanwhile, the filter 17 can filter out the biocontrol microorganism cells. The peristaltic pump 16 continuously and directionally delivers the active substances secreted by the biocontrol microorganisms to the pathogen culture medium to ensure the accuracy of the subsequent molecular detection data.
[0028] As one implementation method in this embodiment, please refer to Figures 1-5 As shown, the first fixing component 2 includes two positioning blocks 21. Each of the adjacent sides of the two positioning blocks 21 is provided with a placement groove 22 that is adapted to the conical bottle 14. A rubber pad 23 is fixedly connected to the inner wall of the placement groove 22. A guide groove 24 is provided at the upper end of the fixing platform 13. A guide block 25 is slidably sleeved inside the guide groove 24. The guide block 25 is fixedly connected to the lower end of the positioning block 21.
[0029] After placing the conical flask 14 in the designated position, push the two positioning blocks 21 in the first fixing component 2 so that the guide block 25 at the lower end of the positioning block 21 slides along the guide groove 24 on the fixing platform 13 until the placement groove 22 on the adjacent side of the two positioning blocks 21 completely fits the outer wall of the conical flask 14. At this time, the rubber pad 23 on the inner wall of the placement groove 22 will be in close contact with the conical flask 14, which can not only limit the conical flask 14, but also reduce the impact on the conical flask 14 when the shaker 12 vibrates through the elastic buffering effect of the rubber pad 23.
[0030] As one implementation method in this embodiment, please refer to Figures 1-6 As shown, the second fixing component 3 includes a first fixing block 31 and a second fixing block 32. The first fixing block 31 and the second fixing block 32 are respectively fixedly connected to two positioning blocks 21. A rotating rod 33 is rotatably sleeved in the middle of the second fixing block 32. A fixing plate 34 is fixedly connected to the end of the rotating rod 33 away from the first fixing block 31. An opening 35 is opened in the middle of the first fixing block 31. A first spring 36 is sleeved on the outside of the rotating rod 33, located between the second fixing block 32 and the fixing plate 34. A slot 37 is opened on the side of the first fixing block 31 away from the second fixing block 32. A locking block 38 is movably engaged inside the slot 37. The locking block 38 is fixedly connected to the rotating rod 33.
[0031] When the two positioning blocks 21 clamp the conical bottle 14, the fixed plate 34 at the end of the rotating rod 33 is pushed first, so that the rotating rod 33 drives the locking block 38 to move closer to the first fixed block 31. At this time, the first spring 36 sleeved on the outside of the rotating rod 33 is compressed. At the same time, one end of the rotating rod 33 passes through the through-hole 35 on the first fixed block 31. Then the rotating rod 33 is rotated so that the locking block 38 is aligned with the slot 37 on the first fixed block 31. Then the fixed plate 34 is released. Under the action of the return elastic force of the first spring 36, the rotating rod 33 drives the locking block 38 to insert into the slot 37, so as to achieve stable locking of the two positioning blocks 21.
[0032] As one implementation method in this embodiment, please refer to Figures 1-9 As shown, the positioning component 4 includes a positioning insert plate 41, an inner groove 42 is provided inside the positioning block 21, the positioning insert plate 41 is movably fitted inside the inner groove 42, a slot 43 is provided at the upper end of the fixing platform 13, the end of the positioning insert plate 41 is movably inserted into the slot 43, a groove is provided on one side of the inner groove 42, a sliding block 44 is movably fitted inside the groove, the sliding block 44 is fixedly connected to the positioning insert plate 41, a first connecting rod 45 is fixedly connected to the inner wall of the groove, a second connecting rod 46 is movably fitted inside the first connecting rod 45, the lower end of the second connecting rod 46 extends out of the first connecting rod 45 and is fixedly connected to the upper end of the sliding block 44, and a second spring 47 located inside the first connecting rod 45 is fixedly connected to the upper end of the second connecting rod 46.
[0033] When the experiment is over and it is necessary to collect the conical flask 14 sample or replace it with a new experimental sample, first release the lock of the second fixing component 3, and then push the positioning block 21 to move it to both sides along the guide groove 24. At this time, in order to avoid the positioning block 21 moving too much or shaking randomly, the position of the opened first fixing component 2 can be fixed by the positioning component 4. When the two positioning blocks 21 slide to the designated position, the elastic thrust applied by the second spring 47 to the second connecting rod 46 causes the positioning insert plate 41 to press down. When the positioning insert plate 41 moves to the position of the slot 43, the elastic thrust applied by the second spring 47 causes the end of the positioning insert plate 41 to be inserted into the slot 43, thereby fixing the position of the positioning block 21 and preventing the positioning block 21 from slipping. During the sliding process of the positioning insert plate 41, the sliding block 44 guides the positioning insert plate 41 by sliding inside the groove, increasing the uniformity of the force on the positioning insert plate 41 and preventing the positioning insert plate 41 from getting stuck due to uneven force, making the device more stable.
[0034] Working principle: Before conducting the screening experiment, the entire device is placed on the workbench 1. The plant pathogens and biocontrol microorganisms to be tested are mixed with the culture medium in a certain proportion and then placed into two conical flasks 14. The conical flasks 14 are then placed at the designated position on the fixed platform 13 in the screening assembly 1. At the same time, the connecting pipe 15 at the lower end of the conical flask 14 is connected to the peristaltic pump 16 on the workbench 11 to ensure that the subsequent liquid transmission channel is unobstructed.
[0035] After the two conical flasks 14 are placed in the designated position, push the two positioning blocks 21 so that the guide block 25 at the lower end of the positioning block 21 slides along the guide groove 24 on the fixed platform 13 until the placement groove 22 on the adjacent side of the two positioning blocks 21 is completely in contact with the outer wall of the conical flask 14. At this time, the rubber pad 23 on the inner wall of the placement groove 22 will be in close contact with the conical flask 14, which on the one hand limits the conical flask 14, and on the other hand reduces the impact on the conical flask 14 when the shaker 12 vibrates through the elastic buffering effect of the rubber pad 23.
[0036] After the positioning block 21 clamps the conical bottle, the fixing plate 34 at the end of the rotating rod 33 is pushed first, so that the rotating rod 33 drives the locking block 38 to move closer to the first fixing block 31. At this time, the first spring 36 sleeved on the outside of the rotating rod 33 is compressed. At the same time, one end of the rotating rod 33 passes through the through-hole 35 on the first fixing block 31. Then the rotating rod 33 is rotated so that the locking block 38 is aligned with the slot 37 on the first fixing block 31. Then the fixing plate 34 is released. Under the action of the return elastic force of the first spring 36, the rotating rod 33 drives the locking block 38 to insert into the slot 37, so as to achieve stable locking of the two positioning blocks 21.
[0037] When the experiment is over and the conical flask 14 sample needs to be collected or a new experimental sample needs to be replaced, first release the lock of the second fixing component 3, and then push the positioning block 21 to move it to both sides along the guide groove 24. At this time, in order to avoid the positioning block 21 moving too much or shaking randomly, the position of the opened first fixing component 2 can be fixed by the positioning component 4. When the two positioning blocks 21 slide to the designated position, the elastic thrust applied by the second spring 47 to the second connecting rod 46 causes the positioning insert plate 41 to press down. When the positioning insert plate 41 moves to the position of the slot 43, the elastic thrust applied by the second spring 47 causes the end of the positioning insert plate 41 to be inserted into the slot 43, thereby fixing the position of the positioning block 21 and preventing the positioning block 21 from slipping. During the sliding process of the positioning insert plate 41, the sliding block 44 guides the positioning insert plate 41 by sliding inside the groove, increasing the uniformity of the force on the positioning insert plate 41 and preventing the positioning insert plate 41 from getting stuck due to uneven force, making the device more stable.
[0038] The above embodiments are only used to illustrate the technical solution of this utility model, and are not intended to limit it.
Claims
1. A device for screening plant pathogenic gene targets, comprising a screening component (1), characterized in that: The upper side of the filtering component (1) is provided with a first fixing component (2) to prevent the filtering component (1) from shaking. Both sides of the first fixing component (2) are provided with a second fixing component (3) to lock the first fixing component (2). Inside the first fixing component (2) is a positioning component (4) to fix the position of the opened first fixing component (2). The first fixing component (2) includes two positioning blocks (21), and each of the two positioning blocks (21) has a placement groove (22) on its adjacent side. A rubber pad (23) is fixedly connected to the inner wall of the placement groove (22).
2. The plant pathogenic gene target screening device according to claim 1, characterized in that: The screening component (1) includes a workbench (11), a shaker (12) is placed on the upper end of the workbench (11), a fixed platform (13) is fixedly connected to the upper end of the shaker (12), two conical flasks (14) are placed on the upper end of the fixed platform (13), the two conical flasks (14) are placed in two placement slots (22) respectively, and the lower ends of the two conical flasks (14) are fixedly connected to connecting pipes (15). A peristaltic pump (16) located on one side of the shaker (12) is placed on the upper end of the workbench (11), and the ends of the two connecting pipes (15) are fixedly connected to the peristaltic pump (16). A filter (17) is fixedly connected to the connecting pipes (15).
3. The plant pathogen pathogenic gene target screening device according to claim 2, characterized in that: The upper end of the fixed platform (13) is provided with a guide groove (24), and a guide block (25) is slidably sleeved inside the guide groove (24). The guide block (25) is fixedly connected to the lower end of the positioning block (21).
4. The plant pathogen pathogenic gene target screening device according to claim 3, characterized in that: The second fixing component (3) includes a first fixing block (31) and a second fixing block (32). The first fixing block (31) and the second fixing block (32) are respectively fixedly connected to two positioning blocks (21). A rotating rod (33) is rotatably sleeved in the middle of the second fixing block (32). A fixing plate (34) is fixedly connected to one end of the rotating rod (33) away from the first fixing block (31). A through-hole (35) is opened in the middle of the first fixing block (31). A first spring (36) is sleeved on the outside of the rotating rod (33) between the second fixing block (32) and the fixing plate (34). A slot (37) is opened on the side of the first fixing block (31) away from the second fixing block (32). A locking block (38) is movably locked inside the slot (37). The locking block (38) is fixedly connected to the rotating rod (33).
5. The plant pathogenic gene target screening device according to claim 3, characterized in that: The positioning component (4) includes a positioning insert plate (41), an inner groove (42) is provided inside the positioning block (21), the positioning insert plate (41) is movably fitted inside the inner groove (42), a slot (43) is provided at the upper end of the fixed platform (13), the end of the positioning insert plate (41) is movably inserted into the slot (43), a groove is provided on one side of the inner groove (42), a sliding block (44) is movably fitted inside the groove, and the sliding block (44) is fixedly connected to the positioning insert plate (41).
6. The plant pathogenic gene target screening device according to claim 5, characterized in that: The inner wall of the groove is fixedly connected to a first connecting rod (45), and a second connecting rod (46) is movably sleeved inside the first connecting rod (45). The lower end of the second connecting rod (46) extends out of the first connecting rod (45) and is fixedly connected to the upper end of the sliding block (44). The upper end of the second connecting rod (46) is fixedly connected to a second spring (47) located inside the first connecting rod (45).