Culture dish for bacteriostatic test

Abstract

The utility model discloses a petri dish for bacteriostasis test, and particularly relates to the technical field of bacteriostasis petri dishes, the petri dish comprises a petri dish body, the top of the petri dish body is in threaded connection with a petri dish cover, a plurality of Oxford cup bodies are fixedly arranged in the petri dish body, a plurality of rectangular through grooves are formed in the middle of each Oxford cup body, and the petri dish cover is in threaded connection with the petri dish body. The diameter of the culture dish body is 90 mm, the height of the culture dish body is 15 mm, the height of the culture dish cover is 13 mm, and the diameter of the Oxford cup body is 6 mm.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of bacteriostatic culture dish, concretely relates to a culture dish for bacteriostatic test. BACKGROUND

[0002] When carrying out bacteriostatic test, culture dish is a common experimental apparatus, which is used for loading culture medium and carrying out microorganism culture. Bacteriostatic test is mainly used for determining the ability of antibiotic, antibacterial peptide and other bacteriostatic substances to inhibit bacterial growth, including qualitative determination diffusion method and quantitative determination dilution method. Diffusion method mainly includes filter paper piece method, flat plate punching method and oxford cup method.

[0003] Oxford cup method is to pour sterilized agar culture medium into culture dish, and wait for it to solidify. Another portion of agar culture medium is cooled to below 50°C, and a proper amount of test bacteria is mixed. The culture medium mixed with test bacteria is added to the solidified culture medium, and wait for it to solidify. Oxford cup is placed vertically on the surface of the culture medium with sterile operation, and is gently pressed to make it in good contact with the surface of the culture medium. The sample to be tested is added in the cup. The culture medium is placed in a 37°C environment for 16-18 hours, and the size of the bacteriostatic ring is observed. The bacteriostatic reagent forms a concentration gradient diffusion area around the oxford cup, in which the bacteria cannot grow in the lowest bacteriostatic concentration range, and this area is called "bacteriostatic ring". The higher the antibacterial activity of the bacteriostatic reagent, the larger the bacteriostatic ring, and the size of the bacteriostatic ring is measured to preliminarily judge the antibacterial ability of the bacteriostatic reagent.

[0004] For example, a kind of oxford cup and culture dish for bacteriostatic experiment provided in Chinese patent application No. CN208532783U, including culture dish cover, culture dish bottom, cup holder and oxford cup, vertical protrusion is arranged on the outer wall upper end of culture dish bottom;The cup holder is inverted disc shape, is arranged on the upper end port of culture dish bottom, the upper surface of cup holder is provided with cup hole, the outer side wall lower part of cup holder is provided with the notch matched with protrusion, the corresponding position of culture dish bottom below oxford cup placement position is marked with scale, and the size of bacteriostatic ring can be observed without measuring it;The bottom of oxford cup is provided with embedded ring, which avoids the phenomenon of bacteriostatic reagent leakage;The corresponding position of culture dish bottom below oxford cup placement position is marked with number, and only the number corresponding to the number is needed when adding bacteriostatic reagent, the number of protrusion and notch is 3, the upper end of oxford cup is provided with handle, and the handle is vertical flat, which is not easy to turn over when being clamped by tweezers. The technical scheme is used for oxford cup and culture dish for bacteriostatic experiment, and the cup holder part makes the oxford cup placement process easy, at the same time, the honeycomb layout of cup hole on cup holder also maximizes the use of the surface of solid culture medium.

[0005] However, the following problems still exist in this technical solution: the Oxford cup is picked up with tweezers and placed into the petri dish. If the Oxford cup is not installed properly, it is easy to fall off and leak liquid. The agar is also easy to perforate the bottom and leak liquid. Moreover, the operation is relatively troublesome. Utility Model Content

[0006] The purpose of this invention is to provide a culture dish for antibacterial testing. An Oxford cup is fixedly installed inside the culture dish body, eliminating the need for an Oxford cup or drilling inside the culture dish body. This prevents leakage, reduces operational procedures and equipment usage, and improves experimental efficiency and accuracy. Furthermore, the rubber pad provides cushioning in case the culture dish body is dropped, enhancing its protective performance, preventing damage upon impact, and extending its service life, thus addressing the aforementioned shortcomings in the technology.

[0007] To achieve the above objectives, this utility model provides the following technical solution: a culture dish for antibacterial testing, comprising a culture dish body, a culture dish lid threadedly connected to the top of the culture dish body, and multiple Oxford cups fixedly disposed inside the culture dish body. Each Oxford cup has multiple rectangular through grooves in the middle, the through grooves being connected to the Oxford cups. The diameter of the culture dish body is 90mm, the height of the culture dish body is 15mm, the height of the culture dish lid is 13mm, the diameter of the Oxford cup is 6mm, the height of the Oxford cup is 15mm, the length of the through groove is 5mm, the height of the Oxford cup above the through groove is 8mm, the height of the Oxford cup below the through groove is 2mm, and 200-300 microliters of antibacterial reagent can be added inside the Oxford cup.

[0008] Preferably, a first support plate is fixedly provided on the outside of the culture dish body, a second support plate is fixedly provided on the top of the first support plate, and an insertion groove is provided at the bottom of the culture dish lid for limiting the position between the culture dish body and the culture dish lid.

[0009] Preferably, the bottom of the culture dish body is provided with a rubber pad to enhance the protective performance of the culture dish body and prevent it from being damaged when dropped.

[0010] Preferably, the inner wall of one side of the culture dish body is machined with graduation lines for measuring the thickness of the culture agar.

[0011] The technical effects and advantages provided by this utility model in the above technical solution are as follows:

[0012] Agar medium can enter the Oxford cup body through the groove on the cup body. The height of the agar medium inside the petri dish body should be higher than the groove to prevent the antibacterial agent inside the Oxford cup body from leaking out. The antibacterial agent inside the Oxford cup body seeps into the agar medium and diffuses through the agar medium to reach the agar medium outside the Oxford cup body. Within a certain concentration range, it inhibits or kills the growth of microorganisms, thus enabling antibacterial effect testing. The petri dish body is placed at 37°C and incubated for 16-18 hours. The size of the inhibition zone near each Oxford cup body is observed through the petri dish body. In this invention, the Oxford cup body is fixedly installed inside the petri dish body, eliminating the need to add Oxford cups or drill holes inside the petri dish body. This prevents leakage, reduces operation procedures and equipment usage, and improves the efficiency and accuracy of the experiment.

[0013] A rubber pad is installed at the bottom of the petri dish. The rubber pad has good elasticity and can cushion the petri dish when it is dropped, which enhances the protective performance of the petri dish, prevents damage when the petri dish is dropped, and extends the service life of the petri dish. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0015] Fig. 1 This is a front view of the present invention;

[0016] Fig. 2 This is a bottom view of the present invention;

[0017] Fig. 3 This is a cross-sectional view of the petri dish body and the petri dish lid of this utility model;

[0018] Fig. 4 This is a schematic diagram of the structure of the petri dish lid of this utility model, which is located away from the petri dish body;

[0019] Fig. 5 This is a schematic diagram of the internal structure of the petri dish body of this utility model;

[0020] Fig. 6 This is a schematic diagram of the structure of the rubber pad of this utility model, which is located away from the petri dish body.

[0021] Explanation of reference numerals in the attached figures:

[0022] 1. Petri dish body, 2. Petri dish lid, 3. Oxford cup body, 4. Through groove, 5. First support plate, 6. Second support plate, 7. Scale line, 8. Insertion groove, 9. Rubber pad. Detailed Implementation

[0023] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings.

[0024] This utility model provides, for example Figs. 1-6 The illustrated petri dish for antibacterial testing includes a petri dish body 1, a petri dish lid 2 threadedly connected to the top of the petri dish body 1, and multiple Oxford cup bodies 3 fixedly disposed inside the petri dish body 1. Each Oxford cup body 3 has multiple rectangular through slots 4 connected to it. The petri dish body 1 has a diameter of 90 mm and a height of 15 mm. The petri dish lid 2 has a height of 13 mm, and the Oxford cup body 3 has a diameter of 6 mm. The height of the cup body 3 is 15mm, the length of the through groove 4 is 5mm, the height of the Oxford cup body 3 above the through groove is 8mm, and the height of the Oxford cup body 3 below the through groove 4 is 2mm. A first support plate 5 is fixedly provided on the outside of the petri dish body 1, and a second support plate 6 is fixedly provided on the top of the first support plate 5. An insertion groove 8 is opened at the bottom of the petri dish lid 2. Graduation lines 7 are processed on one side of the inner wall of the petri dish body 1. 200-300 microliters of antibacterial reagent can be added inside the Oxford cup body 3.

[0025] Pour the agar medium into the petri dish body 1. Determine the volume of the poured agar according to the scale line 7 inside the petri dish body 1. Then pour 200-300 microliters of antibacterial reagent into the Oxford cup body. Immediately insert the insertion slot 8 at the bottom of the petri dish lid 2 into the outside of the second support plate 6 at the top of the first support plate 5. Then rotate the petri dish lid 2 to fix the petri dish lid 2 to the top of the petri dish body 1. The petri dish lid 2 can prevent external dust from entering the petri dish body 1 and avoid affecting the experimental results.

[0026] Agar medium can enter the interior of Oxford cup 3 through the groove 4 on the cup body 3. The height of the agar medium inside the petri dish body 1 should be higher than the groove 4 to prevent leakage of the antibacterial agent inside the Oxford cup 3. The antibacterial agent inside the Oxford cup 3 seeps into the agar medium and diffuses through the agar medium to the agar medium outside the Oxford cup 3, inhibiting or killing the growth of microorganisms within a certain concentration range. This allows for antibacterial effect testing. The petri dish body 1 is placed at 37°C and incubated for 16-18 hours. The size of the inhibition zone near each Oxford cup 3 is observed through the petri dish body 1. In this invention, the Oxford cup 3 is fixedly installed inside the petri dish body 1, eliminating the need to add Oxford cups or drill holes inside the petri dish body 1. This prevents leakage, reduces operation procedures and equipment usage, and improves the efficiency and accuracy of the experiment.

[0027] like Figs. 1-6 As shown, the bottom of the petri dish body 1 is provided with a rubber pad 9. The rubber pad 9, with its good elasticity, can play a buffering role when the petri dish body 1 is dropped, which enhances the protective performance of the petri dish body 1, prevents the petri dish body 1 from being damaged when it falls, and extends the service life of the petri dish body 1.

[0028] The foregoing description only illustrates certain exemplary embodiments of the present invention. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A culture dish for antibacterial testing, comprising a culture dish body (1), characterized in that: The top of the petri dish body (1) is threadedly connected to the petri dish lid (2); The petri dish body (1) is fixedly provided with multiple Oxford cup bodies (3), and each Oxford cup body (3) has multiple rectangular through grooves (4) in the middle, and the through grooves (4) are connected to the Oxford cup body (3).

2. The culture dish for antibacterial testing according to claim 1, characterized in that: The diameter of the petri dish body (1) is 90 mm, the height of the petri dish body (1) is 15 mm, and the height of the petri dish lid (2) is 13 mm.

3. The culture dish for antibacterial testing according to claim 1, characterized in that: The diameter of the Oxford cup body (3) is 6mm, the height of the Oxford cup body (3) is 15mm, the length of the through groove (4) is 5mm, the height of the Oxford cup body (3) above the through groove (4) is 8mm, and the height of the Oxford cup body (3) below the through groove (4) is 2mm.

4. The culture dish for antibacterial testing according to claim 1, characterized in that: The petri dish body (1) is fixedly provided with a first support plate (5), and a second support plate (6) is fixedly provided on the top of the first support plate (5). The bottom of the petri dish cover (2) is provided with an insertion groove (8).

5. A culture dish for antibacterial testing according to claim 4, characterized in that: The insertion slot (8) is adapted to the second support plate (6).

6. The culture dish for antibacterial testing according to claim 1, characterized in that: The bottom of the petri dish body (1) is provided with a rubber pad (9).

7. A culture dish for antibacterial testing according to claim 1, characterized in that: The inner wall of one side of the petri dish body (1) is machined with graduation lines (7).

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