Preparation device of agar plate for immunodiffusion test

Abstract

The utility model provides an agar plate preparation device for immunodiffusion test, which comprises a culture dish, a culture dish cover and a quincuncial pile column arranged on the culture dish cover, and further comprises a middle cover, the middle cover is detachably arranged at an opening of the culture dish, the culture dish cover is covered on the middle cover, and the quincuncial pile column is arranged on the culture dish cover. The middle cover is provided with a penetrating opening for the quincuncial pile column to penetrate through, and the middle cover and the culture dish cover are provided with at least one pair of vertical guide structures matched with each other. The opening track of the culture dish cover is limited, so that the inherent shape of an agar plate is prevented from being damaged when the quincuncial pile column is pulled out of the agar plate, and the preparation efficiency and the qualification rate of the agar plate in the immunodiffusion test process are improved.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental detection technology, and in particular to an agar plate preparation device for immunodiffusion assay. Background Technology

[0002] In immunodiffusion assays, a large number of agar plates with perforated patterns are required. The traditional preparation method involves first preparing solid agar, then manually punching holes using a punch, removing the solid agar from the holes, and finally sealing the bottom with a high-temperature metal seal to produce the agar plate for experimentation. Given the numerous inconveniences of the traditional method, various new techniques have been proposed. A relatively simple and efficient solution is disclosed in patent CN 218089573 U, which involves setting up a petri dish and a petri dish lid. The petri dish and the lid with the perforated pattern are fastened together, allowing the perforated pattern, integrally formed with the lid, to be in place before the agar solution solidifies. After solidification, the perforated pattern is removed, resulting in an agar plate with perforated patterns. Compared to the traditional punching method, the agar plate produced by this invention is simpler and more convenient to operate, and has a higher yield rate. However, in this technical solution, attention must be paid to the gripping posture of the petri dish lid during the opening and closing process. When removing the lid, it must be held vertically to avoid damaging the shape of the perforated holes due to lid displacement. Therefore, although the existing technology improves the preparation efficiency and yield rate of agar plates compared to traditional methods, it largely relies on manual operation. During the preparation process, it is unavoidable that shaking or displacement of the petri dish lid can damage the shape of the perforated holes, thus affecting the yield rate and preparation efficiency. Therefore, the existing technology still has significant room for improvement. Utility Model Content

[0003] (a) Technical problems to be solved

[0004] The purpose of this invention is to provide an agar plate preparation device for immunodiffusion assays, aiming to improve the preparation efficiency and pass rate of agar plates in the immunodiffusion assay process through a more convenient and faster technical means.

[0005] (II) Technical Solution

[0006] An agar plate preparation device for immunodiffusion assay includes a petri dish, a petri dish lid, and a stud post disposed on the petri dish lid. It also includes a middle cover, which is detachably installed at the opening of the petri dish. The petri dish lid is placed on the middle cover. The middle cover has a through-hole for the stud post to pass through. The middle cover and the petri dish lid are provided with at least one pair of mutually compatible vertical guide structures.

[0007] Furthermore, the middle cover includes a cover plate and an upper cavity and a lower cavity respectively disposed on the upper and lower sides of the cover plate. The lower cavity is adapted to the culture dish, and the upper cavity is adapted to the cover of the culture dish.

[0008] Furthermore, the guiding structure is a conductor or a guide groove, and the conductor and the guide groove are adapted to each other.

[0009] Furthermore, the culture cap is also provided with a handle.

[0010] Furthermore, the culture dish and / or culture dish lid and / or plum blossom column are made of high borosilicate glass.

[0011] Preferably, when the petri dish lid, middle lid, and petri dish are assembled, the gap between the plum blossom-shaped column and the bottom of the petri dish is not less than 1 mm.

[0012] Preferably, the inner diameter of the petri dish is 85 mm and the inner height is 15 mm; the inner diameter of the petri dish lid is 95 mm and the inner height is 15 cm; the petri dish lid is provided with 49 cylindrical glass rods, the bottom diameter of the glass rods is 2 mm and the height is 14 mm, the glass rods are attached to the petri dish lid in groups of 7 in a plum blossom shape, and the petri dish lid is provided with a total of 7 groups of glass rods arranged in a plum blossom shape.

[0013] Preferably, the glass rod is bonded and fixed to the petri dish lid with silicone adhesive.

[0014] (III) Beneficial Effects

[0015] This invention proposes an agar plate preparation device for immunodiffusion assays. By incorporating a central cover, the device is mounted on a culture dish. The culture dish lid is inserted into the culture dish through the central cover. At least one pair of mutually compatible vertical guide structures on the central cover and the culture dish lid ensure that the culture dish lid opens vertically. By limiting the opening trajectory of the culture dish lid, the device prevents damage to the inherent shape of the agar plate when the agar plate is pulled out, thereby improving the preparation efficiency and yield of agar plates in the immunodiffusion assay. Attached Figure Description

[0016] Figure 1 This is the basic structural component of the agar plate preparation device of this utility model;

[0017] Figure 2 This is a front view of the bottom of the petri dish lid in this utility model;

[0018] Figure 3 This is a schematic diagram of the structure in the first embodiment of the improved agar plate preparation device of this utility model;

[0019] Figure 4This is a cross-sectional structural diagram of the middle cover of the agar plate preparation device of this utility model;

[0020] Figure 5 This is a schematic diagram of the structure in the second embodiment of the improved agar plate preparation device of this utility model.

[0021] The main attached figures are: 1. Petri dish; 2. Petri dish lid; 3. Plum blossom column; 4. Middle cover; 41. Through-hole; 42. Cover plate; 43. Upper cavity; 44. Lower cavity; 5. Guide structure; 51. Conductor; 52. Guide groove; 6. Handle. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0023] The basic culture dish 1 used in this invention is made of high borosilicate glass, such as... Figure 1 The agar plate preparation apparatus shown consists of two basic parts: a culture dish body and a culture dish lid. The inner diameter of the culture dish body is precisely set to 85 mm, and the inner height is 15 mm; the inner diameter of the culture dish lid is 95 mm, and the inner height reaches 15 cm. To meet the specific requirements of immunodiffusion assays for agar plate preparation, the culture dish lid was specifically modified, combined with the attached... Figure 1 , 2 As shown, 49 cylindrical glass rods are firmly bonded to the inside of the petri dish 1 lid using silicone adhesive. This adhesive is specifically designed for bonding high borosilicate glass, offering significant advantages such as high bonding strength, excellent anti-aging properties, and outstanding high-temperature resistance, while fully complying with environmental standards. These glass rods are made of the same material as petri dish 1, with a base diameter of 2mm and a height of 14mm. They are bonded in groups of seven, arranged in a standard quincunx pattern to the inside of the petri dish 1 lid. A total of seven groups are bonded to the entire petri dish 1 lid, also arranged in a quincunx pattern, with evenly distributed spacing between each group of glass rods and between groups. When the petri dish 1 lid, middle lid 4, and petri dish 1 are assembled, the gap between the quincunx posts 3 and the bottom of petri dish 1 is no less than 1mm, forming a hole bottom. This not only effectively ensures that the glass rods will not fall off during frequent use but also provides a highly precise molding mold for the subsequent preparation of agar plates. Furthermore, a handle 6 is specially added to the petri dish 1 lid, greatly facilitating the handling and operation by laboratory personnel.

[0024] Furthermore, the main design feature of this utility model lies in the middle cover 4, combined with the attached... Figure 3 , 4 As shown, in the first embodiment, the middle cover 4 structure includes a cover plate 42 and an upper cavity 43 and a lower cavity 44 respectively disposed on the upper and lower sides of the cover plate 42. The size of the lower cavity 44 is precisely adapted to the petri dish 1, while the upper cavity 43 fits perfectly with the cover of the petri dish 1. In other embodiments, the material of the middle cover 4 can be either a sturdy and lightweight plastic or metal material, or high borosilicate glass; this ensures that it will not deform during use, thereby stably performing its guiding function. A mutually adapted vertical guiding structure 5 is provided between the middle cover 4 and the cover of the petri dish 1, using a conductor 51 and a guide groove 52 as the guiding structure 5, thereby ensuring that the two can fit tightly together and achieve a smooth guiding effect.

[0025] The structure of the guide groove 52 and the conductor 51 includes, but is not limited to, the following: Figure 3 The first embodiment shown and Figure 5 The second embodiment shown, including the two embodiments described above, includes various guide structures 5, all of which are included within the protection scope of this utility model.

[0026] During device assembly, the lower cavity 44 of the middle cover 4 must first be precisely aligned with the opening of the culture dish 1 to ensure a tight connection and prevent loosening during subsequent operations. Next, place the culture dish 1 cover onto the middle cover 4, ensuring the studs 3 (glass rods) on the cover are precisely aligned with the through-hole 41 on the middle cover 4. Then, slowly lower the culture dish 1 cover until it is completely flush with the middle cover 4. At this point, the guide structure 5 works in concert to strictly restrict the movement of the culture dish 1 cover to the vertical direction only.

[0027] When preparing agar plates, it is essential to strictly adhere to experimental standards. An appropriate amount of agar raw material must be accurately weighed and heated to a completely melted state using suitable heating equipment, ensuring the resulting agar solution is uniform and free of lumps. Then, the melted agar solution is slowly and steadily poured along the edge of the assembled petri dish 1. During this process, extreme care must be taken to prevent overflow and maintain a clean experimental environment. Next, the petri dish 1 containing the agar solution is placed on a stable and vibration-free experimental platform and allowed to cool and solidify naturally. During this period, it is crucial to ensure the apparatus is free from any external interference to avoid adversely affecting the quality of the agar plate. After the agar solution has completely solidified, the experimenter uses the handle 6 to lift the lid of the petri dish 1 vertically and steadily upwards. Due to the constraint of the guide structure 5, the lid of the petri dish 1 can only move vertically, allowing the studs 3 to be vertically pulled out of the agar plate, thus obtaining a complete agar plate with a plum blossom-shaped perforation. Finally, the prepared agar plate is carefully removed for the immunodiffusion experiment. Simultaneously, the used culture dish 1, its lid, and the inner lid 4 are thoroughly cleaned and strictly disinfected to ensure adequate preparation for the next experiment and to guarantee that the experimental equipment remains clean and hygienic at all times. Through the above detailed and rigorous implementation method, the advantages of this novel agar plate preparation device for immunodiffusion experiments are fully demonstrated, significantly improving the preparation efficiency and yield of agar plates, and effectively meeting the professional needs of immunodiffusion experiments.

[0028] This invention proposes an agar plate preparation device for immunodiffusion assays. It includes a central cover 4, which is mounted on a culture dish 1. The culture dish 1 lid is inserted into the culture dish 1 via the central cover 4. At least one pair of mutually compatible vertical guide structures 5 on the central cover 4 and the culture dish 1 lid ensure that the culture dish 1 lid opens vertically. By limiting the opening trajectory of the culture dish 1 lid, the inherent shape of the agar plate is prevented from being damaged when the agar plate is pulled out, thereby improving the preparation efficiency and pass rate of agar plates in the immunodiffusion assay.

[0029] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. An apparatus for preparing agar plates for an immunodiffusion assay, comprising a petri dish, a petri dish lid, and a stud column disposed on the petri dish lid, characterized in that, It also includes a middle cover, which is detachably installed at the opening of the petri dish. The petri dish cover is placed on the middle cover. The middle cover has a through hole for the plum blossom stake to pass through, and the middle cover and the petri dish cover have at least one pair of mutually compatible vertical guide structures.

2. The agar plate preparation apparatus for immunodiffusion assay according to claim 1, characterized in that, The middle cover includes a cover plate and an upper cavity and a lower cavity respectively disposed on the upper and lower sides of the cover plate. The lower cavity is adapted to the culture dish, and the upper cavity is adapted to the cover of the culture dish.

3. The apparatus for preparing agar plates for immunodiffusion assay according to claim 1 or 2, characterized in that, The guiding structure is a conductor or a guide groove, and the conductor and the guide groove are compatible.

4. The agar plate preparation apparatus for immunodiffusion assay according to claim 1, characterized in that, The culture cap is also equipped with a handle.

5. The agar plate preparation apparatus for immunodiffusion assay according to claim 1, characterized in that, The culture dish and / or culture dish lid and / or plum blossom stake are made of high borosilicate glass.

6. The agar plate preparation apparatus for immunodiffusion assay according to claim 1, characterized in that, When the petri dish lid, middle lid and petri dish are assembled, the gap between the plum blossom-shaped column and the bottom of the petri dish is not less than 1 mm.

7. The agar plate preparation apparatus for immunodiffusion assay according to claim 1, characterized in that, The petri dish has an inner diameter of 85 mm and an inner height of 15 mm; the petri dish lid has an inner diameter of 95 mm and an inner height of 15 cm; the petri dish lid contains 49 cylindrical glass rods, each with a bottom diameter of 2 mm and a height of 14 mm. The glass rods are attached to the petri dish lid in groups of 7 in a quincunx pattern. The petri dish lid has a total of 7 groups of glass rods arranged in a quincunx pattern.

8. The agar plate preparation apparatus for immunodiffusion assay according to claim 7, characterized in that, The glass rod is bonded and fixed to the petri dish lid with silicone adhesive.

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