Central cold and hot water bath supply type multi-path temperature-dividing cell culture device

Abstract

The utility model discloses a central cold and hot water bath supply type multi-path temperature-dividing cell culture device. The central cold and hot water bath supply type multi-path temperature-dividing cell culture device comprises a base, a central hot water supply module, a central cold water supply module, a multi-path hot water inlet pipeline, a multi-path cold water inlet pipeline, a plurality of independent adjustable water mixing valves, a plurality of constant-temperature pipe groups and a plurality of cell culture modules, and the water mixing valve in front of the water inlet of each constant-temperature pipe group is respectively connected with a hot water inlet pipeline and a cold water inlet pipeline. According to different set temperatures of all the constant-temperature pipe sets, the water mixing valve can be correspondingly set, so that the temperature of water flowing out of the water mixing valve and entering a water inlet of each constant-temperature pipe set meets the temperature requirement of each constant-temperature pipe set, and each constant-temperature pipe set forms a constant-temperature maintaining area in a corresponding area of the base. And each cell culture module is matched with the corresponding constant-temperature pipe group on the base, so that cell culture environments with different temperatures are realized.

Description

TECHNICAL FIELD

[0001] The utility model relates to a central cold and hot water bath supply type multi-path temperature division cell culture device especially, belongs to micro scale temperature control and biochemical microenvironment construction technical field. BACKGROUND

[0002] The life science field has extremely strict requirements on temperature. In the laboratory or industrial production, temperature control is crucial to ensure the accuracy of experimental results and the high quality of products, especially for temperature-sensitive samples and raw materials such as protease, and small temperature errors will affect the reliability of experimental results and production quality; cell culture also needs to be carried out under certain temperature conditions, and the suitable culture temperature of most mammalian cells is 37°C, but different cell types and experimental purposes have different temperature requirements, such as some tumor cells grow better at slightly lower than 37°C, and some researchers want to study the changes in biological functions of cells under physiological and pathological conditions (37°C vs. different fever conditions). Therefore, there is an urgent need for a simple structure that can achieve independent and high-precision temperature control of multiple small areas.

[0003] The existing temperature control technology has limitations mainly in the following aspects:

[0004] At the incubator level, traditional constant temperature incubators can maintain the overall internal temperature stable, but cannot achieve different temperature control of each culture dish in the incubator. The latest zoned temperature control incubator can only achieve independent temperature control of a larger area in the incubator, and the system structure becomes extremely complex, the system cost is extremely high, and it is still difficult to achieve accurate temperature control of each culture dish.

[0005] At the culture dish level: although there are cell culture dishes with temperature control function, they are mostly for overall temperature control of a single culture dish, and it is difficult to meet the demand for independent temperature control of each well of a 96-well culture dish.

[0006] The difficulties of small-scale multi-zone temperature control mainly lie in the following aspects:

[0007] Space limitation: taking a 96-well culture dish as an example, the size of this type of culture dish is small and the well spacing is close, and it is necessary to integrate heating, cooling, temperature measurement and temperature control elements in a small space under each well, which is technically difficult.

[0008] Temperature interference: the distance between wells is close, and the temperature control of one well may affect the adjacent wells, and it is a technical challenge to maintain the independence and stability of the temperature of each well.

[0009] Accurate temperature measurement and feedback: the temperature in each hole needs to be accurately measured and fed back to the temperature control system in time. The existing temperature measurement technology and sensor are difficult to meet the requirements in terms of accuracy, response speed, miniaturization and the like.

[0010] System integration and cooperation: different temperature controls of each hole need to be realized by integrating multiple temperature control units, power supplies, control circuits and the like into one body, and problems such as system heat dissipation and electromagnetic compatibility need to be solved to ensure long-term stable operation. Practical new content

[0011] The main purpose of the present application is to provide a central cold and hot water bath supply type multi-path temperature separation cell culture device to overcome the shortcomings in the prior art.

[0012] To achieve the foregoing purposes of the practical new type, the technical solution adopted by the present application comprises:

[0013] The first aspect of the embodiment of the present application provides a central cold and hot water bath supply type multi-path temperature separation cell culture device, which comprises:

[0014] A constant temperature maintaining unit comprises a base, a first fluid medium supply module and a second fluid medium supply module. The base has x constant temperature maintaining areas. Each constant temperature maintaining area is provided with a fluid channel group. The x fluid channel groups are independent of each other. Each fluid channel group is connected with the first fluid medium supply module and the second fluid medium supply module simultaneously. The first fluid medium supply module is used for inputting a first fluid medium with a first temperature into the x fluid channel groups. The second fluid medium supply module is used for inputting a second fluid medium with a second temperature into the x fluid channel groups. The flow of the first fluid medium inputted into the x fluid channel groups by the first fluid medium supply module and the flow of the second fluid medium inputted into the x fluid channel groups by the second fluid medium supply module can be independently adjusted. The first fluid medium and the second fluid medium are mixed in the fluid channel group to form a third fluid medium with a third temperature. The x fluid channels and the third fluid medium in the fluid channel form x constant temperature heating structures. In the present application, the second temperature is less than or equal to the third temperature, and the third temperature is less than or equal to the first temperature.

[0015] A cell culture test unit comprises x cell culture test modules. The x cell culture test modules are arranged on the base and correspond to the x constant temperature maintaining areas of the base respectively. Each cell culture test module is in heat conduction cooperation with a constant temperature heating structure. The constant temperature heating structure is used for maintaining the environmental temperature of the cell culture test module in cooperation therewith at a fourth temperature. The fourth temperature is less than or equal to the third temperature. x is greater than or equal to 1.

[0016] The second aspect of the embodiment of the utility model provides a kind of cell culture device, it includes:

[0017] Constant temperature keeping unit, including base and x constant temperature heating structure;

[0018] Cell culture test unit, including x cell culture test module, x cell culture test module is set on the base, the cell culture test module is in heat conduction with one constant temperature heating structure, the constant temperature heating structure is used to keep the ambient temperature of the cell culture test module matched with it at predetermined temperature, the cell culture test module includes test module and sealing cover, the test module has culture cavity and liquid seal groove around the culture cavity, the sealing cover is openably arranged in the liquid seal groove, and completely covers the sample port of the culture cavity, when sealing liquid is arranged in the liquid seal groove and sealing liquid immerses the bottom part of the sealing cover, the sealing cover is sealed with the test module, and, the sealing cover also has water-blocking breathable membrane, the water-blocking breathable membrane covers above the sample port, wherein, x≥1.

[0019] The third aspect of the embodiment of the utility model provides a kind of central cold and hot water bath supply formula multi-path temperature division cell culture device, it includes:

[0020] Constant temperature keeping unit, including base, first fluid medium supply module, second fluid medium supply module, the base has x constant temperature keeping area, each constant temperature keeping area is provided with one fluid channel group, x fluid channel group is independent of each other, each fluid channel group is simultaneously connected with first fluid medium supply module, second fluid medium supply module, first fluid medium supply module is used to input first fluid medium with first temperature in x fluid channel group, second fluid medium supply module is used to input second fluid medium with second temperature in x fluid channel group, the flow of first fluid medium inputted in x fluid channel group by first fluid medium supply module, the flow of second fluid medium inputted in x fluid channel group by second fluid medium supply module can be independently adjusted, first fluid medium and second fluid medium are mixed to form third fluid medium with third temperature in fluid channel group, x fluid channel and third fluid medium in the fluid channel form x constant temperature heating structure, wherein, second temperature≤third temperature≤first temperature, second temperature

[0021] The cell culture test unit comprises x cell culture test modules, the x cell culture test modules are arranged on the base and correspond to x constant temperature maintaining areas of the base respectively, each cell culture test module is in heat conducting cooperation with the constant temperature heating structure, the constant temperature heating structure is used for maintaining the ambient temperature of the cell culture test module cooperating with the constant temperature heating structure at a fourth temperature, the fourth temperature is less than or equal to the third temperature, the cell culture test module comprises a test module and a sealing cover, the test module has a culture cavity and a liquid seal groove arranged around the culture cavity, the sealing cover is arranged in the liquid seal groove in an openable mode and covers the sample port of the culture cavity completely, when the sealing liquid is arranged in the liquid seal groove and the bottom part of the sealing cover is immersed in the sealing liquid, the sealing cover is in sealing cooperation with the test module, and the sealing cover further has a water-resisting and air-permeating film covering above the sample port, and x is greater than or equal to 1.

[0022] Compared with the prior art, the advantages of the utility model include:

[0023] The cell culture test unit comprises x cell culture test modules, the x cell culture test modules are arranged on the base and correspond to x constant temperature maintaining areas of the base respectively, each cell culture test module is in heat conducting cooperation with the constant temperature heating structure, the constant temperature heating structure is used for maintaining the ambient temperature of the cell culture test module cooperating with the constant temperature heating structure at a fourth temperature, the fourth temperature is less than or equal to the third temperature, the cell culture test module comprises a test module and a sealing cover, the test module has a culture cavity and a liquid seal groove arranged around the culture cavity, the sealing cover is arranged in the liquid seal groove in an openable mode and covers the sample port of the culture cavity completely, when the sealing liquid is arranged in the liquid seal groove and the bottom part of the sealing cover is immersed in the sealing liquid, the sealing cover is in sealing cooperation with the test module, and the sealing cover further has a water-resisting and air-permeating film covering above the sample port, and x is greater than or equal to 1.

[0024] The cell culture test unit comprises x cell culture test modules, the x cell culture test modules are arranged on the base and correspond to x constant temperature maintaining areas of the base respectively, each cell culture test module is in heat conducting cooperation with the constant temperature heating structure, the constant temperature heating structure is used for maintaining the ambient temperature of the cell culture test module cooperating with the constant temperature heating structure at a fourth temperature, the fourth temperature is less than or equal to the third temperature, the cell culture test module comprises a test module and a sealing cover, the test module has a culture cavity and a liquid seal groove arranged around the culture cavity, the sealing cover is arranged in the liquid seal groove in an openable mode and covers the sample port of the culture cavity completely, when the sealing liquid is arranged in the liquid seal groove and the bottom part of the sealing cover is immersed in the sealing liquid, the sealing cover is in sealing cooperation with the test module, and the sealing cover further has a water-resisting and air-permeating film covering above the sample port, and x is greater than or equal to 1.

[0025] The cell culture test unit comprises x cell culture test modules, the x cell culture test modules are arranged on the base and correspond to x constant temperature maintaining areas of the base respectively, each cell culture test module is in heat conducting cooperation with the constant temperature heating structure, the constant temperature heating structure is used for maintaining the ambient temperature of the cell culture test module cooperating with the constant temperature heating structure at a fourth temperature, the fourth temperature is less than or equal to the third temperature, the cell culture test module comprises a test module and a sealing cover, the test module has a culture cavity and a liquid seal groove arranged around the culture cavity, the sealing cover is arranged in the liquid seal groove in an openable mode and covers the sample port of the culture cavity completely, when the sealing liquid is arranged in the liquid seal groove and the bottom part of the sealing cover is immersed in the sealing liquid, the sealing cover is in sealing cooperation with the test module, and the sealing cover further has a water-resisting and air-permeating film covering above the sample port, and x is greater than or equal to 1. BRIEF DESCRIPTION OF DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the accompanying drawings needed to be used in the embodiments or prior art description will be briefly introduced. Obviously, the accompanying drawings in the following description are only some embodiments described in the present application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0027] Figure 1 、 Figure 2 、 Figure 3 is a structure schematic diagram of a central cold and hot water bath supply type multi-path temperature division cell culture device provided by the embodiment 1 of the present application;

[0028] Figure 4 is a structure schematic diagram of a cell culture device provided by the embodiment 2 of the present application;

[0029] Figure 5 is a structure schematic diagram of a single cell culture test module in a cell culture device provided by the embodiment 2 of the present application;

[0030] Figure 6 is a structure schematic diagram of a cell culture device provided by the embodiment 2 of the present application;

[0031] Figure 7 is a structure schematic diagram of a central cold and hot water bath supply type multi-path temperature division cell culture device provided by the embodiment 3 of the present application. DETAILED DESCRIPTION

[0032] In view of the deficiencies in the prior art, the present inventors have long studied and practiced a large amount of experiments, and thus have proposed the technical solutions of the present application. The technical solutions, implementation processes and principles will be further explained as follows.

[0033] A first aspect of the embodiments of the present application provides a central cold and hot water bath supply type multi-path temperature division cell culture device, which comprises:

[0034] The constant temperature holding unit comprises a base, a first fluid medium supply module and a second fluid medium supply module. The base has x constant temperature holding areas, each of which is provided with a fluid channel group. The x fluid channel groups are independent of each other. Each fluid channel group is simultaneously connected with the first fluid medium supply module and the second fluid medium supply module. The first fluid medium supply module is used for inputting a first fluid medium with a first temperature into the x fluid channel groups. The second fluid medium supply module is used for inputting a second fluid medium with a second temperature into the x fluid channel groups. The flow of the first fluid medium input into the x fluid channel groups by the first fluid medium supply module and the flow of the second fluid medium input into the x fluid channel groups by the second fluid medium supply module can be independently adjusted. The first fluid medium and the second fluid medium are mixed in the fluid channel groups to form a third fluid medium with a third temperature. The x fluid channels and the third fluid medium in the fluid channels form x constant temperature heating structures. The second temperature is less than or equal to the third temperature, and the third temperature is less than or equal to the first temperature.

[0035] The cell culture test unit comprises x cell culture test modules. The x cell culture test modules are arranged on the base and correspond to the x constant temperature holding areas of the base, respectively. Each cell culture test module is in heat transfer cooperation with a constant temperature heating structure. The constant temperature heating structure is used for keeping the environment temperature of the cell culture test module in cooperation with the constant temperature heating structure at a fourth temperature. The fourth temperature is less than or equal to the third temperature, and x is greater than or equal to 1.

[0036] It should be noted that the environment temperature of the cell culture test module can be understood as the temperature of the culture medium in the cell culture test module.

[0037] Further, each fluid channel group comprises y flow channels, and y is greater than or equal to 1. When y is equal to 1, at least part of the flow channel is in a wave shape or a spiral shape structure. When y is greater than or equal to 2, the y flow channels are connected with each other. The y flow channels are arranged side by side or cross each other. Alternatively, z flow channels in the y flow channels are arranged side by side or cross each other. The flow channel is in a straight line type, an arc line type, a wave shape or a spiral shape structure, and y is greater than z and z is greater than or equal to 2.

[0038] Further, each fluid channel group has a fluid medium inlet and a fluid medium outlet. The first fluid medium supply module comprises x first fluid medium input pipes. The second fluid medium supply module comprises x second fluid medium input pipes. The fluid medium inlet of each fluid channel group is connected with a first fluid medium input pipe and a second fluid medium input pipe. The first fluid medium input pipe and the second fluid medium input pipe are both provided with a flow adjusting valve.

[0039] Further, the fluid medium inlet of each of the fluid channel groups is connected to a first fluid medium input pipe and a second fluid medium input pipe through a water mixing valve.

[0040] In a more specific embodiment, the central cold and hot water bath supplied multi-path temperature divided cell culture device further comprises a fluid medium recycling processing unit, the fluid medium recycling processing unit comprises a collection and storage module, a temperature adjusting module and a fluid medium circulating module, the fluid medium outlets of the x fluid channel groups are connected to the collection and storage module through fluid medium output pipes, the temperature adjusting module is used for adjusting the temperature of the fluid medium collected by the collection and storage module to a first temperature and / or a second temperature, and the fluid medium circulating module is used for delivering the fluid medium with the temperature adjusted to the first temperature to the first fluid medium supply module and delivering the fluid medium with the temperature adjusted to the second temperature to the second fluid medium supply module.

[0041] In a more specific embodiment, the central cold and hot water bath supplied multi-path temperature divided cell culture device further comprises a temperature monitoring unit, which is used for monitoring at least one of the temperature of the first fluid medium output by the first fluid medium supply module, the temperature of the second fluid medium output by the second fluid medium supply module, the temperature of the third medium in the fluid channel group and the temperature in the cell culture test module.

[0042] A second aspect of the embodiment of the utility model provides a cell culture device, which comprises:

[0043] The constant temperature maintaining unit comprises a base and x constant temperature heating structures.

[0044] The cell culture test unit comprises x cell culture test modules, the x cell culture test modules are arranged on the base, the cell culture test module is in heat conduction cooperation with the constant temperature heating structure, the constant temperature heating structure is used for maintaining the environment temperature of the cell culture test module cooperating with the constant temperature heating structure at a predetermined temperature, the cell culture test module comprises a test module and a sealing cover, the test module has a culture cavity and a liquid seal groove arranged around the culture cavity, the sealing cover is arranged in the liquid seal groove in an openable mode and covers the sample port of the culture cavity completely, when the liquid seal groove is arranged with a sealing liquid and the sealing liquid immerses the bottom part of the sealing cover, the sealing cover is in sealing cooperation with the test module, and the sealing cover further has a water-blocking and air-permeable film, the water-blocking and air-permeable film covers above the sample port, and x is greater than or equal to 1.

[0045] In a more specific embodiment, the cell culture device further comprises a sealing liquid supplementing unit, the sealing liquid supplementing unit is connected to the liquid seal groove of the x test modules and is used for injecting the sealing liquid into the liquid seal groove.

[0046] Further, the sealing liquid supplementing unit comprises a sealing liquid supply module and a sealing liquid supplementing tank, the sealing liquid supply module is directly connected with the sealing liquid supplementing tank, the sealing liquid supplementing tank is connected with x liquid sealing tanks, and the liquid level height in the sealing liquid supplementing tank is always consistent with that of the x liquid sealing tanks.

[0047] Further, the sealing liquid supplementing tank is arranged on the base, and a sealing liquid valve is arranged on a supplementing pipe between the liquid sealing tank and the sealing liquid supplementing tank.

[0048] Further, a liquid level sensor is arranged in the sealing liquid supplementing tank.

[0049] Exemplarily, the water-blocking and air-permeable membrane can be a polytetrafluoroethylene (PTFE) membrane, a Gore-Tex (a multifunctional fabric invented and produced by W.L. Gore & Associates, Inc.), a specially treated non-woven fabric, etc., which are known in the art and will not be specifically described herein.

[0050] A third aspect of the embodiment of the utility model provides a central cold and hot water bath supply type multi-path temperature dividing cell culture device, it includes:

[0051] The constant temperature maintaining unit comprises a base, a first fluid medium supply module and a second fluid medium supply module, the base has x constant temperature maintaining areas, each constant temperature maintaining area is provided with a fluid channel group, x fluid channel groups are independent of each other, each fluid channel group is connected with the first fluid medium supply module and the second fluid medium supply module simultaneously, the first fluid medium supply module is used for inputting first fluid medium with a first temperature into x fluid channel groups, the second fluid medium supply module is used for inputting second fluid medium with a second temperature into x fluid channel groups, the flow of first fluid medium inputted into x fluid channel groups by the first fluid medium supply module and the flow of second fluid medium inputted into x fluid channel groups by the second fluid medium supply module can be independently adjusted, the first fluid medium and the second fluid medium are mixed in the fluid channel group to form third fluid medium with a third temperature, x constant temperature heating structures are formed by x fluid channels and the third fluid medium in the fluid channel, wherein, second temperature ≤ third temperature ≤ first temperature, second temperature < first temperature.

[0052] The cell culture test unit comprises x cell culture test modules, which are arranged on the base and correspond to x constant temperature maintaining areas of the base respectively. Each cell culture test module is in heat conducting cooperation with the constant temperature heating structure, which is used to maintain the environment temperature of the cell culture test module in cooperation with the constant temperature heating structure at a fourth temperature, which is less than the third temperature. The cell culture test module comprises a test module and a sealing cover. The test module has a culture cavity and a liquid seal groove arranged around the culture cavity. The sealing cover is arranged in the liquid seal groove and covers the sample port of the culture cavity completely. When the liquid seal groove is filled with sealing liquid and the bottom part of the sealing cover is immersed in the sealing liquid, the sealing cover is in sealing cooperation with the test module. The sealing cover also has a water-resistant and air-permeable film, which covers the sample port. x is greater than or equal to 1.

[0053] It should be noted that the environment temperature of the cell culture test module can be understood as the temperature of the culture medium in the cell culture test module.

[0054] Further, each fluid channel group comprises y flow channels, y is greater than or equal to 1. When y is equal to 1, at least part of the flow channel is in wave or spiral structure. When y is greater than or equal to 2, the y flow channels are connected, arranged side by side or intersected. Alternatively, z flow channels in the y flow channels are arranged side by side or intersected. The flow channel is in straight line, arc, wave or spiral structure. y is greater than z, and z is greater than or equal to 2.

[0055] Further, each fluid channel group has a fluid medium inlet and a fluid medium outlet. The first fluid medium supply module comprises x first fluid medium input pipes, and the second fluid medium supply module comprises x second fluid medium input pipes. The fluid medium inlet of each fluid channel group is connected with a first fluid medium input pipe and a second fluid medium input pipe. The first fluid medium input pipe and the second fluid medium input pipe are provided with flow regulating valves.

[0056] Further, the fluid medium inlet of each fluid channel group is connected with a first fluid medium input pipe and a second fluid medium input pipe through a water mixing valve.

[0057] In a more specific embodiment, the central cold and hot water bath supplied multi-path temperature separated cell culture device further comprises a fluid medium recycling processing unit, the fluid medium recycling processing unit comprises a collection storage module, a temperature adjusting module and a fluid medium circulating module, the fluid medium outlets of the x fluid channel groups are connected with the collection storage module through fluid medium output pipes, the temperature adjusting module is used for adjusting the temperature of the fluid medium collected by the collection storage module to a first temperature and / or a second temperature, and the fluid medium circulating module is used for delivering the fluid medium with the adjusted first temperature to the first fluid medium supply module and delivering the fluid medium with the adjusted second temperature to the second fluid medium supply module.

[0058] In a more specific embodiment, the central cold and hot water bath supplied multi-path temperature separated cell culture device further comprises a temperature monitoring unit, the temperature monitoring unit is used for monitoring at least one of the temperature of the first fluid medium output by the first fluid medium supply module, the temperature of the second fluid medium output by the second fluid medium supply module, the temperature of the third medium in the fluid channel group and the temperature in the cell culture test module, and the temperature monitoring unit comprises a temperature sensor, a temperature table and the like.

[0059] In a more specific embodiment, the central cold and hot water bath supplied multi-path temperature separated cell culture device further comprises a sealing liquid supplementing unit, the sealing liquid supplementing unit is connected with the liquid sealing grooves of the x test modules and is used for injecting sealing liquid into the liquid sealing grooves.

[0060] Further, the sealing liquid supplementing unit comprises a sealing liquid supply module and a sealing liquid supplementing groove, the sealing liquid supply module is directly connected with the sealing liquid supplementing groove, the sealing liquid supplementing groove is connected with the x liquid sealing grooves, and the liquid levels in the x liquid sealing grooves and the sealing liquid supplementing groove are always consistent.

[0061] Further, the sealing liquid supplementing groove is arranged on the base, and a sealing liquid valve is arranged on a supplementing pipe between the liquid sealing groove and the sealing liquid supplementing groove.

[0062] Further, a liquid level sensor is arranged in the sealing liquid supplementing groove.

[0063] Further, the sealing liquid supply module is the same as the first fluid medium supply module or the second fluid medium supply module.

[0064] The technical scheme, implementation process and principles will be further explained in combination with the accompanying drawings and specific implementation cases. Unless specifically stated, the pump, various function valves, liquid level sensors, temperature sensors, controllers, numerical control systems, circuit structures and the like mentioned in the embodiments of the present application are known in the art and can be obtained from the market. The structure, product model and working principle of the structure are not limited.

[0065] It should be noted that the first fluid medium, the second fluid medium and the third fluid medium used to realize the constant-temperature heating structure in the embodiments of the present application are the same fluid medium, and the three are only different in temperature. The "first", "second" and "third" are only used to distinguish them in naming. The fluid medium used to realize temperature control in the embodiments of the present application can be water, oil, silicone oil or glycerol aqueous solution, and the sealing liquid used to realize liquid sealing can also be water, oil and the like.

[0066] Embodiment 1

[0067] Please refer to Figure 1 , Figure 2 and Figure 3 , a central cold and hot water bath supply type multi-path temperature separation cell culture device, comprising a base 110, x constant-temperature tube groups 120 (i.e. x fluid channel groups, the lumen inside the constant-temperature tube group is the fluid channel, the same below), a hot water supply module 210 (i.e. a first fluid medium supply module, the same below), a cold water supply module 310 (i.e. a second fluid medium supply module, the same below), a hot water inlet pipeline 220, a cold water inlet pipeline 320 and x cell culture test modules,

[0068] The x constant-temperature tube groups 120 are arranged at intervals in the inside of the base 110. Each constant-temperature tube group 120 has one water inlet (i.e. a fluid medium inlet, the same below) and one water outlet (i.e. a fluid medium inlet, the same below). The hot water supply module 210 is in communication with the hot water inlet pipeline 220, and the hot water inlet pipeline 220 is in communication with the x water inlets. The cold water supply module 310 is in communication with the cold water inlet pipeline 320, and the cold water inlet pipeline 320 is in communication with the x water inlets. The hot water provided by the hot water supply module 210 and the cold water provided by the cold water supply module 310 are mixed in proportion in the x constant-temperature tube groups 120. The constant-temperature tube groups 120 and the water inside the constant-temperature tube groups 120 form a constant-temperature heating structure. Each constant-temperature heating structure forms a constant-temperature maintaining area in the base 110. The x cell culture test modules are arranged on the base 110 and are in heat-conducting cooperation with the x constant-temperature heating structures. Each constant-temperature heating structure independently maintains the environmental temperature of the cell culture test module cooperating therewith constant, Figure 1x = 4 in the central hot and cold water bath supply type multi-path temperature separation cell culture device shown in the middle.

[0069] It should be noted that the cell culture test module in the embodiment is a functional module capable of realizing cell culture and monitoring and collecting related parameters in the culture medium, which can be known in the art, and will not be described as an improvement point in the embodiment. The ambient temperature of the cell culture test module mainly refers to the temperature of the culture medium in the cell culture test module.

[0070] In the embodiment, the constant temperature pipe group 120 is a heat conducting pipe group. Exemplarily, the constant temperature pipe group 120 can be a metal pipe, for example, a copper pipe, etc. The part of the base 110 directly contacting the constant temperature pipe group 120 and the cell culture test module is also formed of a material with good heat conductivity, and the material of the base 110 between the adjacent two constant temperature pipe groups 120 is preferably a heat insulation material to reduce the temperature influence between the adjacent two constant temperature maintaining areas. Exemplarily, the material with good heat conductivity in the base 110 can be metal or ceramic, etc., and the heat insulation material in the base 110 can be aerogel, glass fiber, etc. These materials and components achieving the heat conducting function and the heat insulation function can be realized by the materials and technologies known in the art, which will not be explained and limited too much herein.

[0071] In the embodiment, the hot water supply module 210 includes a hot water tank and a hot water supply pump, the hot water inlet pipeline 220 includes a hot water main pipe 221 and x hot water branch pipes 222, the hot water tank is communicated with the hot water main pipe 221 through the hot water supply pump, each hot water branch pipe 222 is respectively communicated with the hot water main pipe 221 and the water inlet of a constant temperature pipe group 120, and a flow valve is arranged on the hot water branch pipe 222. A flow valve or an on-off valve, etc. can also be arranged on the hot water main pipe 221. Specifically, the hot water tank can be integrated with a heating mechanism and a temperature sensor. The temperature sensor is used to monitor the temperature of the hot water in the hot water tank, and the heating mechanism is used to heat the water in the hot water tank to keep the temperature of the hot water in the hot water tank at a first temperature. It should be noted that the temperature sensor can be directly connected with the heating mechanism to form a temperature feedback circuit structure. The temperature sensor, the heating mechanism, and the signal feedback between the heating mechanism and the temperature sensor are known in the art, which will not be limited too much herein.

[0072] In the present embodiment, the structure of the cold water supply module 310 is substantially the same as that of the hot water supply module 210, and the cold water inlet pipeline 320 includes one cold water main pipe 321 and x cold water branch pipes 322. The cold water tank is in communication with the cold water main pipe 321 via a cold water supply pump. Each cold water branch pipe 322 is in communication with the cold water main pipe 321 and the water inlet of one constant temperature pipe group 120, respectively. The cold water branch pipe 322 is provided with a flow valve. The cold water main pipe 321 can also be provided with a flow valve or an on-off valve, etc. Of course, the water inlet of each constant temperature pipe group 120 is in communication with one cold water branch pipe 322 and one hot water branch pipe 222 via one mixing valve, respectively. The cold water and the hot water are mixed in the mixing valve and then enter the constant temperature pipe group 120. Specifically, the cold water tank can be integrated with a refrigeration mechanism and a temperature sensor. The temperature sensor is used to monitor the temperature of the cold water in the cold water tank. The refrigeration mechanism is used to heat the water in the cold water tank to keep the temperature of the cold water in the cold water tank at a second temperature.

[0073] In the present embodiment, each constant temperature pipe group 120 includes one constant temperature water pipe. The middle part of the constant temperature water pipe serves as the main functional part. The middle part of the constant temperature water pipe presents a meandering wave shape structure. The hot water with the first temperature input via the hot water branch pipe 222 and the cold water with the second temperature input via the cold water branch pipe 322 are mixed in the constant temperature water pipe and form water with a third temperature. The third temperature is between the first temperature and the second temperature. Through the flow valve, the flow rates of the hot water with the first temperature and the cold water with the second temperature entering each constant temperature water pipe can be adjusted to change the third temperature of the water in the constant temperature water pipe, so as to form different heating temperatures in the x constant temperature maintaining areas. It can be understood that when the flow rate of the cold water or the hot water input into a certain constant temperature water pipe is 0, the temperature of the water in the constant temperature water pipe is the first temperature or the second temperature.

[0074] In the present embodiment, temperature sensors can also be arranged in the x constant temperature pipe groups 120 and the x cell culture test modules to monitor the temperature, respectively. The working parameters of the flow valves on the cold water branch pipes 322 and the hot water branch pipes 222 are adjusted according to the monitored temperature to finally realize the adjustment of the environmental temperature of the x cell culture test modules. It can be understood that in order to realize the automation of temperature monitoring and temperature adjustment, a controller can be arranged. The controller is electrically connected with all the temperature sensors and the flow valves in the device and realizes real-time monitoring and adjustment of the working parameters of the flow valves according to the monitored temperature information. Of course, the controller and the related numerical control program and circuit structure, etc. are known in the art and are not particularly limited here.

[0075] In the present embodiment, the water discharged from the water outlets of the x constant-temperature tube groups 120 can be directly discharged, can be discharged to a water storage tank, or can be transported to a circulating water tank. That is, the central hot and cold water bath supply type multi-path constant-temperature cell culture device in the present embodiment can further comprise a hot water circulating water tank and a cold water circulating water tank. The water outlets of the x constant-temperature tube groups 120 are respectively in communication with the hot water circulating water tank and the cold water circulating water tank. The hot water circulating water tank is further in communication with the hot water tank, and the cold water circulating water tank is further in communication with the cold water tank. The hot water circulating water tank is integrated with a heating mechanism and a circulating pump, and can heat the water in the hot water circulating water tank to a first temperature and then transport the water back to the hot water tank. The cold water circulating water tank is integrated with a refrigeration mechanism and a circulating pump, and can cool the water in the cold water circulating water tank to a second temperature and then transport the water back to the cold water tank.

[0076] It should be noted that the cold water provided by the cold water supply module 310 is preferably cold water (i.e., ice water mixture) at 0°C. The cold water at 0°C does not have temperature fluctuations, so the temperature fluctuations of the mixed warm water can be reduced, and it is easier to form a temperature-stable temperature zone in the base, thereby providing a more stable cell culture environment.

[0077] Embodiment 2

[0078] Please refer to Figure 4 A cell culture device comprising: a constant-temperature base 400 and x cell culture test modules 500, the x cell culture test modules 500 being arranged on the constant-temperature base 400 and being in heat-conducting cooperation with the constant-temperature base 400, the constant-temperature base 400 being used to maintain the environmental temperature of the x cell culture test modules 500 at a predetermined temperature, Figure 4 The cell culture device in the present embodiment shown in the above table has x = 4. The constant-temperature base 400 in the present embodiment can adopt a structure known in the art, which can be an electric heating constant-temperature base or a water bath heating constant-temperature base, etc., which is not limited herein.

[0079] In the present embodiment, please refer to Figure 4 、 Figure 5The cell culture testing module 500 comprises a testing module 510 and a sealing cover 540, the testing module 510 is arranged on the constant temperature base 400, the testing module 510 has a culture cavity 520 and a liquid seal groove 530 arranged around the culture cavity 520, the sealing cover 540 is arranged in the liquid seal groove 530 in an openable manner and covers the sample port of the culture cavity 520 completely, the sealing cover 540 also has a water-blocking and air-permeable film which covers above the sample port, when the sealing liquid is arranged in the liquid seal groove 530 and the sealing liquid immerses the bottom part of the sealing cover 540, the sealing cover 540 is sealed with the testing module 510, so that the culture cavity 520 is configured as a relatively sealed environment for only gas exchange. In the embodiment, the sealing cover 540 can be rotatably connected with the constant temperature base 400 through a rotating shaft, and the sealing cover 540 can be switched between the open state and the closed state by rotating the sealing cover 540.

[0080] In the embodiment, please refer to Figure 4 、 Figure 5 and Figure 6 In order to improve the efficiency of the liquid seal of the cell culture testing module 500, the cell culture device in the embodiment further comprises a sealing liquid supply module, a sealing liquid supplement tank 610 and a supplement pipeline 630, the sealing liquid supplement tank 610 is arranged on the constant temperature base 400, the sealing liquid supply module, the sealing liquid supplement tank 610 and the liquid seal grooves 530 of the x cell culture testing modules 500 are communicated through the supplement pipeline 630, the sealing liquid provided by the sealing liquid supply module is transported to the sealing liquid supplement tank 610 and the x liquid seal grooves 530, since the sealing liquid supplement tank 610 and the x liquid seal grooves 530 are directly communicated, the liquid levels of the sealing liquid in the sealing liquid supplement tank 610 and the x liquid seal grooves 530 will always remain the same, the liquid level of the sealing liquid in the x liquid seal grooves 530 can be known by observing the liquid level of the sealing liquid in the sealing liquid supplement tank 610, so that the accurate regulation and control of the delivery amount of the sealing liquid of the x liquid seal grooves 530 can be realized.

[0081] In the embodiment, the liquid supplement pipeline 630 comprises a main liquid supplement pipeline 631 and x liquid supplement branch pipelines 632, the main liquid supplement pipeline 631 is communicated with the liquid seal supplement tank 610, the x liquid supplement branch pipelines 632 are respectively communicated with the main liquid supplement pipeline 631, the x liquid supplement branch pipelines 632 are also respectively communicated with the x liquid seal tanks 530, and a liquid inlet valve is arranged on each liquid supplement branch pipeline 632, of course, a flow valve can also be arranged on the main liquid supplement pipeline 631, and the liquid level of the sealing liquid in each liquid seal tank 530 can also be controlled through the flow valve on the main liquid supplement pipeline 631 and the liquid inlet valve on the liquid supplement branch pipeline 632, so that the sealing liquid is prevented from overflowing or polluting the cell culture test module 500. Specifically, in order to accurately know the liquid level of the sealing liquid in the liquid seal tank 530, a liquid level sensor 620 is also arranged in the liquid seal supplement tank 610, the liquid level in the liquid seal supplement tank 610 is monitored through the liquid level sensor 620, and thus the liquid level in the x liquid seal tanks 530 is known. Of course, the liquid level sensor 620 can also be connected with the sealing liquid supply module, and the sealing liquid supply module can start or stop the sealing liquid supply according to the information fed back by the liquid level sensor 620.

[0082] In the embodiment, the x liquid seal tanks 530 can also be connected with the sealing liquid supply module through a sealing liquid circulation pipeline, and the sealing liquid in the x liquid seal tanks 530 can be transported back to the sealing liquid supply module through the sealing liquid circulation pipeline, and the circulation use of the sealing liquid is realized.

[0083] Embodiment 3

[0084] Please refer to Figure 7 The cell culture test module in the embodiment is basically same as that in embodiment 1, and the difference is that the cell culture test module in the embodiment adopts the cell culture test module in embodiment 2.

[0085] The cell culture device provided by the embodiment of the utility model adopts the mode of constant temperature circulating water control on the base, creates a relatively stable temperature environment for the cell culture test module, specifically, with the help of controllable cold water and hot water supply, the cold water and the hot water are mixed in the fluid passage group in the base to obtain temperature constant warm water, at the same time, by controlling the flow of each hot water and each cold water, the temperature of the water in the fluid passage group can be changed, not only the environmental temperature of each test module can be accurately adjusted, thereby providing relatively constant temperature conditions for the test, but also multiple temperature zones with different constant temperatures can be formed in the base to create different test temperature environments in the same test device.

[0086] The cell culture device provided by the embodiment of the utility model utilizes the liquid supplement pump to supplement the sealing liquid to the sealing liquid tank, after the test module is installed, the sealing liquid valve is opened, the sealing liquid flows into the sealing tank, and liquid sealing is realized.

[0087] It should be understood that the above embodiments are only to illustrate the technical concept and characteristics of the present application, the purpose is to enable those skilled in the art to understand the content of the present application and to implement it, and cannot limit the protection scope of the present application. Any equivalent changes or modifications made in accordance with the spirit and essence of the present application shall be covered within the protection scope of the present application.

Claims

1. A central cold and hot water bath supplied multi-path temperature-divided cell culture device, characterized in that, The application relates to a constant-temperature holding unit and a cell culture testing unit. The constant-temperature holding unit comprises a base, a first fluid medium supply module and a second fluid medium supply module, the base is provided with x constant-temperature holding areas, each constant-temperature holding area is provided with a fluid channel group, x fluid channel groups are independent of each other, each fluid channel group is connected with the first fluid medium supply module and the second fluid medium supply module at the same time, the first fluid medium supply module is used for inputting first fluid medium with a first temperature into the x fluid channel groups, the second fluid medium supply module is used for inputting second fluid medium with a second temperature into the x fluid channel groups, the flow of the first fluid medium input into the x fluid channel groups by the first fluid medium supply module and the flow of the second fluid medium input into the x fluid channel groups by the second fluid medium supply module can be independently adjusted, the first fluid medium and the second fluid medium are mixed in the fluid channel group to form third fluid medium with a third temperature, x constant-temperature heating structures are formed by the x fluid channels and the third fluid medium in the fluid channels, wherein the second temperature is less than or equal to the third temperature and less than the first temperature. The cell culture testing unit comprises x cell culture testing modules, the x cell culture testing modules are arranged on the base and correspond to the x constant-temperature holding areas of the base respectively, each cell culture testing module is in heat conduction cooperation with a constant-temperature heating structure, the constant-temperature heating structure is used for keeping the environmental temperature of the cell culture testing module cooperating with the constant-temperature heating structure at a fourth temperature, the fourth temperature is less than or equal to the third temperature, and x is greater than or equal to 1.

2. The central water bath multi-channel temperature-controlled cell culture device according to claim 1, wherein: Each fluid channel group comprises y flow channels, y is greater than or equal to 1, at least part of the flow channels is in a wave shape or a spiral shape when y is equal to 1, the y flow channels are connected with each other when y is greater than or equal to 2, the y flow channels are arranged side by side or cross each other, or z flow channels in the y flow channels are arranged side by side or cross each other, the flow channels are in a straight line type, an arc line type, a wave shape or a spiral shape, y is greater than z and z is greater than or equal to 2.

3. The multi-pass temperature-controlled cell culture device of claim 1 or 2, wherein: Each fluid channel group is provided with a fluid medium inlet and a fluid medium outlet, the first fluid medium supply module comprises x first fluid medium input pipes, the second fluid medium supply module comprises x second fluid medium input pipes, the fluid medium inlet of each fluid channel group is connected with a first fluid medium input pipe and a second fluid medium input pipe, and flow adjusting valves are arranged on the first fluid medium input pipes and the second fluid medium input pipes; And / or, the fluid medium inlet of each fluid channel group is connected with a first fluid medium input pipe and a second fluid medium input pipe through a water mixing valve. And / or, the central cold and hot water bath supply type multi-path temperature-divided cell culture device further comprises a fluid medium recycling processing unit, the fluid medium recycling processing unit comprises a collection and storage module, a temperature adjusting module and a fluid medium circulating module, the fluid medium outlets of the x fluid channel groups are connected with the collection and storage module through fluid medium output pipes, the temperature adjusting module is used for adjusting the temperature of the fluid medium collected by the collection and storage module to a first temperature and / or a second temperature, and the fluid medium circulating module is used for delivering the fluid medium with the temperature adjusted to the first temperature to the first fluid medium supply module and delivering the fluid medium with the temperature adjusted to the second temperature to the second fluid medium supply module. And / or, the central cold and hot water bath supply type multi-path temperature-divided cell culture device further comprises a temperature monitoring unit, which is used for monitoring at least one of the temperature of the first fluid medium output by the first fluid medium supply module, the temperature of the second fluid medium output by the second fluid medium supply module, the temperature of the third medium in the fluid channel group and the temperature in the cell culture test module.

4. A cell culture device, characterized by, Comprise: A constant temperature maintaining unit comprising a base and x constant temperature heating structures; A cell culture test unit comprising x cell culture test modules, the x cell culture test modules are arranged on the base, and each cell culture test module is in heat conduction cooperation with one constant temperature heating structure, the constant temperature heating structure is used for maintaining the environment temperature of the cell culture test module cooperating with the constant temperature heating structure at a predetermined temperature, the cell culture test module comprises a test module and a sealing cover, the test module has a culture cavity and a liquid seal groove arranged around the culture cavity, the sealing cover is arranged in the liquid seal groove in an openable manner and covers the sample port of the culture cavity completely, the sealing cover is in sealing cooperation with the test module when sealing liquid is arranged in the liquid seal groove and the bottom part of the sealing cover is immersed in the sealing liquid, and the sealing cover further has a water-blocking and air-permeable film covering above the sample port, wherein x is greater than or equal to 1.

5. The cell culture device of claim 4, wherein, Further comprise: A sealing liquid supplementing unit, which is connected with the liquid seal grooves of the x test modules and is used for injecting sealing liquid into the liquid seal grooves.

6. The cell culture device of claim 5, wherein: The sealing liquid supplementing unit comprises a sealing liquid supply module and a sealing liquid supplementing tank, the sealing liquid supply module is directly connected with the sealing liquid supplementing tank, the sealing liquid supplementing tank is connected with the x liquid seal grooves, and the liquid level height in the sealing liquid supplementing tank is always consistent with that in the x liquid seal grooves; And / or, the sealing liquid supplementing tank is arranged on the base, and a sealing liquid valve is arranged on a supplementing pipe between the liquid seal groove and the sealing liquid supplementing tank; And / or, a liquid level sensor is arranged in the sealing liquid supplementing tank.

7. A central cold and hot water bath supplied multi-pass temperature split cell culture device, characterized in that, Comprise: The constant temperature holding unit comprises a base, a first fluid medium supply module and a second fluid medium supply module, the base has x constant temperature holding areas, each of the constant temperature holding areas is provided with a fluid channel group, x fluid channel groups are independent of each other, each of the fluid channel groups is connected with the first fluid medium supply module and the second fluid medium supply module at the same time, the first fluid medium supply module is used for inputting a first fluid medium with a first temperature into x fluid channel groups, the second fluid medium supply module is used for inputting a second fluid medium with a second temperature into x fluid channel groups, the flow of the first fluid medium input into x fluid channel groups by the first fluid medium supply module and the flow of the second fluid medium input into x fluid channel groups by the second fluid medium supply module can be independently adjusted, the first fluid medium and the second fluid medium are mixed in the fluid channel group to form a third fluid medium with a third temperature, x fluid channels and the third fluid medium in the fluid channel form x constant temperature heating structures, wherein the second temperature is less than or equal to the third temperature and the third temperature is less than or equal to the first temperature, and the second temperature is less than the first temperature; The cell culture test unit comprises x cell culture test modules, the x cell culture test modules are arranged on the base and correspond to the x constant temperature holding areas of the base respectively, each of the cell culture test modules is in heat conduction cooperation with a constant temperature heating structure, the constant temperature heating structure is used for keeping the ambient temperature of the cell culture test module cooperating with the constant temperature heating structure at a fourth temperature, the fourth temperature is less than or equal to the third temperature, the cell culture test module comprises a test module and a sealing cover, the test module has a culture cavity and a liquid seal groove arranged around the culture cavity, the sealing cover is arranged in the liquid seal groove in an openable mode and covers the sample port of the culture cavity completely, when the liquid seal groove is provided with a sealing liquid and the sealing liquid immerses the bottom part of the sealing cover, the sealing cover is in sealing cooperation with the test module, and the sealing cover further has a water-resisting and air-permeating film covering above the sample port, and x is greater than or equal to 1.

8. The central hot and cold water bath supplied multi-path temperature divided cell culture device according to claim 7, wherein: Each of the fluid channel groups comprises y flow channels, y is greater than or equal to 1, at least part of the flow channels is in a wave shape or a spiral shape when y is equal to 1, y flow channels are connected with each other when y is greater than 2, the y flow channels are arranged side by side or cross each other, or z flow channels in the y flow channels are arranged side by side or cross each other, the flow channels are in a straight line type, an arc line type, a wave shape or a spiral shape, y is greater than z and z is greater than or equal to 2.

9. The multi-pass temperature-controlled cell culture device of claim 7 or 8, wherein: Each of the fluid channel groups has a fluid medium inlet and a fluid medium outlet, the first fluid medium supply module comprises x first fluid medium input pipes, the second fluid medium supply module comprises x second fluid medium input pipes, the fluid medium inlet of each of the fluid channel groups is connected with a first fluid medium input pipe and a second fluid medium input pipe, and flow adjusting valves are arranged on the first fluid medium input pipes and the second fluid medium input pipes. And / or, the fluid medium inlet of each of the fluid channel groups is connected to a first fluid medium input pipe and a second fluid medium input pipe through a water mixing valve; And / or, the central cold and hot water bath supplied multi-path temperature divided cell culture device further comprises a fluid medium recycling processing unit, the fluid medium recycling processing unit comprises a collection and storage module, a temperature adjusting module and a fluid medium circulating module, the fluid medium outlets of the x fluid channel groups are connected to the collection and storage module through a fluid medium output pipe, the temperature adjusting module is used for adjusting the temperature of the fluid medium collected by the collection and storage module to a first temperature and / or a second temperature, and the fluid medium circulating module is used for delivering the fluid medium with the temperature adjusted to the first temperature to the first fluid medium supply module and delivering the fluid medium with the temperature adjusted to the second temperature to the second fluid medium supply module; And / or, the central cold and hot water bath supplied multi-path temperature divided cell culture device further comprises a temperature monitoring unit, the temperature monitoring unit is used for monitoring at least one of the temperature of the first fluid medium output by the first fluid medium supply module, the temperature of the second fluid medium output by the second fluid medium supply module, the temperature of the third medium in the fluid channel group and the temperature in the cell culture test module.

10. The central hot and cold water bath-supplied multi-path temperature-divided cell culture device according to claim 7, wherein, Further comprising: A sealing liquid supplementing unit, the sealing liquid supplementing unit is connected to the liquid sealing grooves of the x test modules and is used for injecting sealing liquid into the liquid sealing grooves; And / or, the sealing liquid supplementing unit comprises a sealing liquid supply module and a sealing liquid supplementing groove, the sealing liquid supply module is directly connected to the sealing liquid supplementing groove, the sealing liquid supplementing groove is connected to the x liquid sealing grooves, and the liquid levels in the x liquid sealing grooves and the sealing liquid supplementing groove are always consistent; And / or, the sealing liquid supplementing groove is arranged on the base, and a sealing liquid valve is arranged on a supplementing pipe between the liquid sealing groove and the sealing liquid supplementing groove; And / or, a liquid level sensor is arranged in the sealing liquid supplementing groove; And / or, the sealing liquid supply module is the same as the first fluid medium supply module or the second fluid medium supply module.

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