Long-time-sequence microorganism enrichment sampling cup

Abstract

The utility model discloses a long-time sequence microorganism enrichment sampling cup which comprises a cylindrical cup body and a cup cover in threaded sealing connection with the cup body, a multi-stage filtering assembly is arranged in the cup body, liquid injection openings are formed in the cup body and the cup cover, and a multi-stage liquid injection pipeline, a multi-stage liquid outlet pipeline and a pressure relief pipeline are arranged outside the cup body and the cup cover. A plug is arranged at a port of the liquid outlet pipeline; the liquid outlet pipelines and the pressure relief pipelines are located on the same side, and the liquid injection pipelines are located on the opposite sides. The utility model has the beneficial effects that through a three-stage membrane filtering structure, impurities are effectively removed, and the effective concentration of a sampled product is improved; a plurality of liquid injection ports are arranged, after a sample is filtered, multi-stage injection of the stationary liquid is realized, the stationary liquid is ensured to be in full contact with the sample, the impurity content is reduced, and the microorganism purity and the sampling efficiency are improved; a plurality of pressure release valves are arranged, so that after the sample enrichment operation is completed, the pressure of the sampling cup is quickly released, the sampling process is simplified, and the scientific investigation experiment efficiency is improved; the device adopts a modular design, is simple and stable in structure, is convenient to operate and sample, and is particularly suitable for carrying and transporting scenes of underwater vehicles in scientific investigation experiments.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of bioengineering technology, concretely is a long time sequence microorganism enrichment sampling cup. BACKGROUND

[0002] With the deepening of marine scientific research, the collection and enrichment of microbial samples have important research value in the fields of marine biology, environmental science and bioengineering. Marine microorganisms not only play a key role in ecological balance, but also show broad application prospects in the fields of new drug research and development, enzyme engineering and environmental remediation. Therefore, accurately and effectively collecting microbial samples, especially for long-term enrichment, is crucial to improving the quality of scientific research results.

[0003] At present, the existing microbial sampling device usually adopts the filtering and concentrating method for the enrichment of microbial samples. However, the existing technology mainly has the following problems:

[0004] 1. Low filtering efficiency and poor impurity removal effect: The traditional sampling device adopts a single membrane filtration system, which is easy to be mixed with impurities during the filtering process, resulting in low sample concentration, insufficient purity and affecting the accuracy of subsequent experiments.

[0005] 2. Uneven injection of fixing liquid and low microbial purity: The existing device cannot achieve sufficient contact when injecting the fixing liquid, resulting in unsatisfactory fixing effect, high impurity content and affecting the purity of microorganisms and the quality of samples.

[0006] 3. Complicated pressure relief operation, affecting sampling efficiency: The existing sampling equipment lacks effective pressure relief design after completing the filtering and enrichment, resulting in complicated operation and long time consumption during the sampling process, which reduces the efficiency of scientific research.

[0007] 4. Complex structure and poor portability: The structure design of some sampling devices is complex, with poor stability, and it is difficult to adapt to the carrying requirements of underwater carriers in marine scientific research, which brings inconvenience to actual use. INVENTION CONTENTS

[0008] The main purpose of the utility model is to provide a long time sequence microorganism enrichment sampling cup with good impurity removal effect and high effective sample concentration.

[0009] The long time sequence microorganism enrichment sampling cup provided by the utility model comprises a cylindrical cup body and a cup cover in threaded sealing connection with the cup body, a plurality of filtering assemblies are arranged in the cup body, a liquid injection port is formed on the cup body and the cup cover, a plurality of liquid injection pipelines, a plurality of liquid outlet pipelines and a pressure relief pipeline are arranged outside the cup body and the cup cover, a plug is arranged at the port of the liquid outlet pipeline, the liquid outlet pipelines and the pressure relief pipeline are located on the same side, and the liquid injection pipeline is located on the opposite side.

[0010] In an embodiment of the sampling cup, the bottom end of the inner cavity of the cup body is provided with a downward connecting port, in which a one-way valve only allowing liquid injection is arranged.

[0011] In an embodiment of the sampling cup, the top end of the inner cavity of the cup cover is provided with an upward connecting port, in which a one-way valve only allowing liquid injection is arranged.

[0012] In an embodiment of the sampling cup, the filter assembly is equally arranged in three groups from top to bottom in the inner cavity of the cup body, and each group of filter assemblies comprises a filter bushing, a gasket and a backing plate.

[0013] In an embodiment of the sampling cup, a plurality of filter bushings are arranged on the side wall of the cup body at the same interval, and the upper and lower ends of the filter bushings are provided with O-shaped gaskets; the lower end of the filter bushing is clamped by the gasket at the lower end of the filter bushing and the gasket at the upper end of the lower group of filter bushings.

[0014] In an embodiment of the sampling cup, the liquid injection pipeline and the liquid outlet pipeline are each provided with three parts from top to bottom, and each part is arranged above the filter membrane of each group of filter assemblies.

[0015] The sampling cup has the following beneficial effects:

[0016] 1. The three-stage membrane filtering structure effectively removes impurities and improves the effective concentration of the sampled sample.

[0017] 2. Multiple liquid injection ports are configured, so that after the sample filtering is completed, the multi-stage injection of the fixing liquid is realized, the fixing liquid and the sample are fully contacted, the impurity content is reduced, and the microbial purity and the sampling efficiency are improved.

[0018] 3. Multiple pressure relief valves are arranged, so that after the sample enrichment operation is completed, the sampling cup is quickly depressurized, the sampling process is simplified, and the scientific research and experiment efficiency is improved.

[0019] 4. The modular design is adopted, the structure is simple and stable, the operation and sampling are convenient, and the modular design is particularly suitable for the carrying and transportation scene of the underwater carrier in the scientific research and experiment. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 It is a perspective structural schematic view of an embodiment of the utility model.

[0021] Figure 2 It is a sectional structural schematic view of Figure 1 . DETAILED DESCRIPTION

[0022] With reference to the drawings of the embodiments of the present application, the related technical solutions will be clearly and completely described, obviously, the described embodiments are only a part of the embodiments, rather than all the embodiments. Based on the embodiments of the present application, all the other embodiments obtained by the ordinary skilled in the art without creative labor are within the protection scope of the present application.

[0023] As shown in Figure 1 and Figure 2 The present embodiment discloses a long-time sequence microorganism enrichment sampling cup, which comprises a cup body 1, a cup cover 2 and a filter assembly.

[0024] The cup body 1 is a cylindrical hollow cup, and a threaded circular cup cover 2 is arranged on the cup body 1. The cup cover can be removed after the filtration and enrichment work is completed, which is convenient for subsequent sampling and operation.

[0025] A downward connecting port is arranged at the bottom end of the inner cavity of the cup body 1, and a one-way valve 11 for injecting liquid only is arranged in the connecting port. An upward connecting port is arranged at the top end of the inner cavity of the cup cover 2, and a one-way valve 21 for injecting liquid only is arranged in the connecting port. The one-way valves control the one-way flow of liquid, ensure the smooth progress of the filtration process, and avoid backflow.

[0026] The filter assembly is arranged in the inner cavity of the cup body 1 in three groups from top to bottom at equal intervals. Each group of the filter assembly comprises a filter bushing 31, a gasket 32 and a backing plate 33.

[0027] A plurality of filter bushings 31 are arranged on the side wall of the cup body 1 at the same interval, and an O-shaped gasket 32 is arranged at the upper and lower ends of the filter bushing. A backing plate 33 is arranged below the filter bushing, and the backing plate is clamped by the gasket at the lower end of the filter bushing and the gasket at the upper end of the lower group of filter bushings.

[0028] The three groups of filter assemblies constitute a three-stage filtration system, which gradually removes impurities in the liquid and effectively improves the purity and effective concentration of the sample.

[0029] A liquid injection pipeline 12, a liquid outlet pipeline 13 and a pressure relief pipeline 14 are arranged on the outer wall of the cup body 1 and are in communication.

[0030] The liquid outlet pipeline 13 and the pressure relief pipeline 14 are located on the same side, and the liquid injection pipeline 12 is located on the opposite side.

[0031] The liquid injection pipeline 12 and the liquid outlet pipeline 13 are each provided with three places from top to bottom, and are each correspondingly arranged above the three groups of filter membranes of the filter assembly 3. The multi-stage liquid injection port design ensures that the fixing liquid and the sample are in full contact, reduces the impurity content, and further improves the purity of the microorganism sample.

[0032] A plug that can be manually removed is placed at the port of the liquid outlet pipeline 13.

[0033] The use method of the sampling cup is as follows:

[0034] 1. The seawater or other microbial sample liquid is pumped into the outer shell through the top of the sampling cup and enters the three-stage filtration system;

[0035] 2. The liquid passes through the three groups of filtration assemblies in the three-stage filtration system in turn;

[0036] 3. The fixed liquid is injected into the filtration system in multiple stages through the liquid injection pipelines arranged at different positions of the outer shell, and the filtration enrichment operation is performed;

[0037] 4. After the filtration enrichment operation is completed, the plugs at the outlet pipe ports are taken out in turn, and the pressure is released through the pressure relief valve arranged on the outer shell;

[0038] 5. After the pressure relief is completed, the cup cover is taken out, and the sample can be taken out for operation, which is convenient for subsequent experimental analysis.

[0039] The long-time sequence microbial enrichment sampling cup has the following characteristics:

[0040] 1. Through the three-stage membrane filtration design, impurities are gradually removed, and the effective concentration and purity of the sample are improved.

[0041] 2. Multiple liquid injection ports ensure that the fixed liquid and the sample are in full contact, realize efficient fixation, and improve the sample quality.

[0042] 3. The pressure relief valve design simplifies the pressure relief operation, improves the sampling efficiency, and is suitable for rapid operation of field scientific investigation experiments.

[0043] 4. The modular design makes the overall structure simple and stable, easy to operate, and is particularly suitable for carrying out scientific investigation experiments on underwater carriers.

[0044] Finally, it should be pointed out that the above only describes the preferred embodiments of the present application and is not intended to limit the present application. Although the foregoing embodiments have been described in detail, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent replacements to some technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A long time-series microorganism enrichment sampling cup, characterized by: It includes a cylindrical cup body and a cup cover threadedly sealed with the cup body, a multi-stage filter assembly is arranged in the cup body, a liquid inlet is formed on the cup body and the cup cover, a multi-stage liquid injection pipeline, a multi-stage liquid outlet pipeline and a pressure relief pipeline are arranged outside the cup body and the cup cover, a plug is arranged at the end of the liquid outlet pipeline, each liquid outlet pipeline and pressure relief pipeline is located on the same side, and the liquid injection pipeline is located on the opposite side.

2. The long duration microorganism concentration sampling cup of claim 1, wherein: A downward connecting port is arranged at the bottom end of the inner cavity of the cup body, and a one-way valve only allowing liquid injection is arranged in the connecting port.

3. The long duration microorganism concentration sampling cup of claim 1, wherein: An upward connecting port is arranged at the top end of the inner cavity of the cup cover, and a one-way valve only allowing liquid injection is arranged in the connecting port.

4. The long duration microorganism concentration sampling cup of claim 1, wherein: Three groups of filter assemblies are equidistantly arranged in the inner cavity of the cup body from top to bottom, each group of filter assemblies includes a filter bushing, a gasket and a backing plate.

5. The long duration microorganism concentration sampling cup of claim 4, wherein: A plurality of filter bushings are equidistantly arranged on the side wall of the cup body, and O-shaped gaskets are arranged at the upper and lower ends of the filter bushings; the backing plate is arranged below the filter bushings, and the backing plate is clamped by the gasket at the lower end of the filter bushing and the gasket at the upper end of the lower filter bushing.

6. The long duration microorganism concentration sampling cup of claim 4, wherein: The liquid injection pipeline and the liquid outlet pipeline are each provided with three parts from top to bottom, and each part is arranged above the filter membrane of each stage of filter assemblies.

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