Microfluidic closed microstructure for knee joint motor injury gene detection

Abstract

The embodiment of the utility model discloses a microfluidic closed microstructure for knee joint motor injury gene detection. According to one specific embodiment, the microfluidic closed microstructure for knee joint motor injury gene detection comprises a plastic substrate, wherein a sample injection groove, a liquid buffer chamber, a phase change material chamber, a second preset number of reaction grooves and a waste liquid collection chamber are arranged on the plastic substrate; each reaction tank connecting flow channel in the second preset number of reaction tank connecting flow channels comprises at least one micro-flow valve; the phase-change material chamber comprises a third preset number of microcavities, and phase-change materials contained in the third preset number of microcavities can control closing of micro-flow valves contained in the second preset number of reaction tank connecting runners through phase change. According to the implementation mode, the sealing performance can be improved, the time consumption of gene detection is shortened, the accuracy of gene detection is improved, wasted phase change materials are reduced, and the situation that the microfluidic closed microstructure deforms is reduced.

Description

TECHNICAL FIELD

[0001] Embodiments of the present disclosure relate to the field of microfluidic chips, and in particular, to a microfluidic closed microstructure for detecting knee movement injury genes. BACKGROUND

[0002] Using microfluidic technology to detect the genes of knee movement injury can improve the efficiency of gene detection. At present, when using microfluidic technology to detect the genes of knee movement injury, the commonly used way is to detect the genes of knee movement injury according to a microfluidic chip using mechanical valve sealing technology (a mechanical valve opens and closes a microfluidic channel in a microfluidic chip through mechanical movement) or to detect the genes of knee movement injury according to a microfluidic chip using heat sealing technology (a heating element is used to heat a sealing material to seal a microfluidic channel).

[0003] However, when detecting genes by the commonly used way, the following technical problems often exist:

[0004] When detecting genes by a microfluidic chip using mechanical valve sealing technology, the wear of mechanical parts causes the sealing performance of the microfluidic chip to be low, and the movement of the mechanical valve causes disturbance of liquid in the microfluidic channel, resulting in poor reaction effect. When detecting genes by a microfluidic chip using heat sealing technology, the time-consuming of heating the sealing material is long, and inaccurate temperature control causes the structure of the microfluidic chip to be deformed or the sealing material to be damaged, resulting in waste of the sealing material and long time-consuming of gene detection.

[0005] The above information disclosed in this BACKGROUND section is only for the purpose of enhancing the understanding of the background of the present disclosure and, therefore, it can contain information that does not form the prior art that is already known in this country to those ordinary skilled in the art. SUMMARY

[0006] The summary of the present disclosure is used to introduce the concepts in a simple form, which will be described in detail in the following detailed description. The summary of the present disclosure is not intended to identify key or essential features of the claimed technical solutions, nor is it intended to be used to limit the scope of the claimed technical solutions.

[0007] Some embodiments of the present disclosure propose a microfluidic closed microstructure for detecting knee movement injury genes to solve one or more of the technical problems mentioned in the above BACKGROUND section.

[0008] Some embodiments of the present disclosure provide a microfluidic closed microstructure for knee motion injury gene detection, characterized in that the microfluidic closed microstructure comprises a plastic substrate, a first preset number of third connecting flow channels, a second preset number of reaction groove connecting flow channels, a siphon flow channel and a liquid shunt flow channel, wherein the plastic substrate is provided with a sample injection groove, a liquid buffer chamber, a phase change material chamber, the second preset number of reaction grooves and a waste liquid collection chamber; the lower end of the sample injection groove and the upper end of the liquid buffer chamber are connected by the siphon flow channel; the lower end of the liquid buffer chamber and the waste liquid collection chamber are connected by the liquid shunt flow channel, one side of the liquid shunt flow channel is provided with a first preset number of chamber inlet shunt ports, the other side of the liquid shunt flow channel is provided with a second preset number of groove inlet shunt ports, the lower end of the phase change material chamber and the first preset number of chamber inlet shunt ports are connected by the first preset number of third connecting flow channels, the second preset number of reaction grooves and the second preset number of groove inlet shunt ports are connected by the second preset number of reaction groove connecting flow channels, each of the second preset number of reaction groove connecting flow channels comprises at least one microfluidic valve; the phase change material chamber comprises a third preset number of microcavities, wherein each of the third preset number of microcavities contains phase change material, and the phase change material contained in the third preset number of microcavities can control the closing of each microfluidic valve contained in the second preset number of reaction groove connecting flow channels by phase change.

[0009] Optionally, the microfluidic closed microstructure for knee motion injury gene detection further comprises a sample injection port, a first connecting flow channel, a phase change material injection port and a second connecting flow channel.

[0010] Optionally, the upper end of the sample injection groove and the sample injection port are connected by the first connecting flow channel.

[0011] Optionally, the upper end of the phase change material chamber and the phase change material injection port are connected by the second connecting flow channel.

[0012] Optionally, the reaction grooves in the second preset number of reaction grooves and the groove inlet shunt ports in the second preset number of groove inlet shunt ports are one-to-one corresponding, and the reaction grooves in the second preset number of reaction grooves and the corresponding groove inlet shunt ports are connected by a reaction groove connecting flow channel.

[0013] Optionally, the phase change material is paraffin.

[0014] Optionally, the plastic substrate is circular.

[0015] Optionally, each microfluidic valve contained in the second preset number of reaction groove connecting flow channels is semicircular.

[0016] Optionally, the diameter of the sample injection port is 2mm.

[0017] Optionally, each of the second preset number of reaction slots is circular in shape, each of the third preset number of microcavities contains phase change material that can undergo phase change at a preset temperature, each of the second preset number of reaction slot connection channels is provided with at least one microvalve made of elastic material and shaped as a semicircle, the sample injection slot, the liquid buffer chamber, the phase change material chamber, the second preset number of reaction slots, and the waste liquid collection chamber are distributed in a diffusion shape with a preset center position, the sample injection slot is located in the innermost circumferential area of the microfluidic closed microstructure for knee joint movement injury gene detection, the phase change material chamber and the liquid buffer chamber are located in the intermediate circumferential area of the microfluidic closed microstructure for knee joint movement injury gene detection, the second preset number of reaction slots and the waste liquid collection chamber are located in the outermost circumferential area of the microfluidic closed microstructure for knee joint movement injury gene detection, in the use state, the microfluidic closed microstructure for knee joint movement injury gene detection is installed on a carrier, and when the carrier is running, the carrier drives the microfluidic closed microstructure for knee joint movement injury gene detection to rotate clockwise or counterclockwise based on the preset center position.

[0018] Optionally, one side of the liquid shunt channel is provided with a target number of inlet slot shunt ports for connecting with the target number of reaction slots, the target number of reaction slots are standby reaction slots, the target number of inlet slot shunt ports are externally covered by a covering assembly, the covering assembly is slidable, in the use state, the slidable covering assembly exposes the inlet slot shunt port to further connect the inlet slot shunt port and the reaction slot, sliding the covering assembly can cover the damaged reaction slot corresponding to the inlet slot port, and a piston is installed on the sample injection port of the microfluidic closed microstructure, in the use state, the piston is pulled out to inject the sample solution.

[0019] Some embodiments of the present disclosure provide a microfluidic closed microstructure for knee movement injury gene detection, which can improve the sealing performance of the microfluidic chip and the reaction effect of the microfluidic chip, reduce the waste of sealing materials and the deformation of the microfluidic chip, and further shorten the time consumption of gene detection. Specifically, the reasons for the poor sealing performance and reaction effect of the microfluidic chip, the waste of sealing materials and the deformation of the microfluidic chip, and the long time consumption of gene detection are as follows: when the microfluidic chip detects genes by using mechanical valve sealing technology, the wear of mechanical parts causes the low sealing performance of the microfluidic chip, and the movement of the mechanical valve causes liquid disturbance in the microfluidic channel, resulting in poor reaction effect. When the microfluidic chip detects genes by using heat sealing technology, the time consumption of heating the sealing material is long, and the inaccurate temperature control causes the deformation of the structure of the microfluidic chip or the damage of the sealing material, resulting in the waste of sealing materials and the long time consumption of gene detection. Based on this, some embodiments of the present disclosure provide a microfluidic closed microstructure for knee movement injury gene detection, which includes a plastic substrate, a first preset number of third connection flow channels, a second preset number of reaction groove connection flow channels, a siphon flow channel and a liquid shunt flow channel. The plastic substrate is provided with a sample injection groove, a liquid buffer chamber, a phase change material chamber, a second preset number of reaction grooves and a waste liquid collection chamber. The lower end of the sample injection groove is connected to the upper end of the liquid buffer chamber through the siphon flow channel. The lower end of the liquid buffer chamber is connected to the waste liquid collection chamber through the liquid shunt flow channel. One side of the liquid shunt flow channel is provided with a first preset number of chamber inlet shunt ports, and the other side of the liquid shunt flow channel is provided with a second preset number of groove inlet shunt ports. The lower end of the phase change material chamber is connected to the first preset number of chamber inlet shunt ports through the first preset number of third connection flow channels. The second preset number of reaction grooves are connected to the second preset number of groove inlet shunt ports through the second preset number of reaction groove connection flow channels. Each of the second preset number of reaction groove connection flow channels includes at least one microfluidic valve. The phase change material chamber includes a third preset number of microcavities. Each of the third preset number of microcavities contains phase change material. The phase change material contained in the third preset number of microcavities can control the closing of each microfluidic valve contained in the second preset number of reaction groove connection flow channels by phase change.In one aspect, instead of closing the microfluidic flow channel by using mechanical valve sealing technology or using thermal sealing technology, after the phase change material is heated, the phase change material changes phase and flows into each microfluidic valve contained in the second preset number of reaction tank connecting flow channels by capillary action and centrifugal force, to fill each microfluidic valve, so that the microfluidic valve is converted to a closed state under the filling and solidification of the phase change material, and the phase change material can push the microfluidic valve to the inlet of the reaction tank to close the reaction tank, and due to the difference between the density of the solution in the reaction tank and the density of the phase change material and the small structure of the microfluidic sealing microstructure for knee joint movement injury gene detection, the flow of the phase change material into the reaction tank is hindered, so that the phase change material cannot enter the reaction tank, thereby achieving automatic sealing of the reaction tank and sealing of the microfluidic flow channel. In the working state, the microfluidic sealing microstructure for knee joint movement injury gene detection is installed on the rotating stage, so that the phase change material can flow into each microfluidic valve contained in the second preset number of reaction tank connecting flow channels by centrifugal force. In another aspect, the microfluidic sealing microstructure is a microstructure, so the microfluidic sealing microstructure has a large surface area to volume ratio, can quickly conduct and dissipate heat, so that the phase change material quickly reaches and maintains the melting point, thereby reducing the time spent on heating the phase change material and the time spent on gene detection, thereby reducing the waste of microfluidic chip materials and the damage to the microfluidic chip structure. In another aspect, the microfluidic sealing microstructure includes a sample injection tank, a liquid buffer chamber, a phase change material chamber, the second preset number of reaction tanks, and a waste liquid collection chamber, so that the integrated design of the microfluidic sealing microstructure can simplify the steps of gene detection, thereby further reducing the time spent on gene detection and improving the accuracy of gene detection. Therefore, the microfluidic sealing microstructure can improve the sealing performance, shorten the time spent on gene detection, improve the accuracy of gene detection, and reduce the waste of phase change materials and reduce the deformation of the microfluidic sealing microstructure. BRIEF DESCRIPTION OF DRAWINGS

[0020] The above and other features, advantages, and aspects of embodiments of the present disclosure will become more apparent by describing in detail some embodiments thereof with reference to the attached drawings. The same or similar components have the same or similar reference numbers throughout the drawings. It is to be understood that the drawings are schematic, and elements and components are not necessarily drawn to scale.

[0021] Figure 1 is a structure diagram of a hollow cavity structure and each flow channel in a microfluidic sealing microstructure for knee joint movement injury gene detection according to some embodiments of the present disclosure;

[0022] Figure 2is a structural schematic diagram of a microfluidic closed microstructure for knee movement injury gene detection of some embodiments of the present disclosure.

[0023] Figure 3 is a structural schematic diagram of a microfluidic closed microstructure placement stage for knee movement injury gene detection of some embodiments of the present disclosure. DETAILED DESCRIPTION

[0024] Embodiments of the present disclosure will be described in more detail with reference to the drawings. Although some embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure can be implemented in various forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that the present disclosure can be more thoroughly and completely understood. It should be understood that the drawings of the present disclosure and the embodiments are only for exemplary purposes and are not intended to limit the scope of protection of the present disclosure.

[0025] It should also be noted that, for the sake of brevity, only the parts of the drawings that are relevant to the present disclosure are shown. The embodiments in the present disclosure and the features in the embodiments can be combined with each other without conflict.

[0026] It should be noted that the terms "first", "second", and the like in the present disclosure are only used to distinguish different devices, modules, or units, and are not intended to limit the functions performed by these devices, modules, or units or the mutual dependency therebetween.

[0027] It should be noted that the terms "one", "multiple" in the present disclosure are illustrative and not restrictive, and those skilled in the art should understand that, unless otherwise explicitly stated in the context, it should be understood as "one or more".

[0028] The names of the messages or information exchanged between the devices in the embodiments of the present disclosure are only for illustrative purposes and are not intended to limit the scope of the messages or information.

[0029] The present disclosure will be described in detail below with reference to the drawings and in conjunction with the embodiments.

[0030] Figure 1 is a structural diagram of a microfluidic closed microstructure for knee movement injury gene detection of an embodiment of the present disclosure. Figure 1 including a sample injection inlet 1, a first connecting flow channel 2, a sample injection groove 3, a siphon flow channel 4, a liquid buffer chamber 5, a liquid shunt flow channel 6, a waste liquid collection chamber 7, a phase change material injection inlet 8, a second connecting flow channel 9, a phase change material chamber 10, a third connecting flow channel 11, a microfluidic valve 12, a reaction groove connecting flow channel 13, and a reaction groove 14.

[0031] In some embodiments, the microfluidic closed microstructure for knee joint movement injury gene detection can include a plastic substrate 01, a first preset number of third connection flow channels 11, a second preset number of reaction groove connection flow channels 13, a siphon flow channel 4 and a liquid shunt flow channel 6. The plastic material of the above-mentioned plastic substrate 01 can include but is not limited to: polymethyl methacrylate (PMMA), polycarbonate (PC), polystyrene (PS) and polypropylene (PP). The materials of the above-mentioned sample injection groove 3, the above-mentioned phase change material chamber 10, the above-mentioned reaction groove 14, the above-mentioned liquid buffer chamber 5 and the waste liquid collection chamber 7 can be the same as the material of the above-mentioned plastic substrate 01. Here, the size of the above-mentioned first preset number of third connection flow channels 11, the second preset number of reaction groove connection flow channels 13, the first connection flow channel 2, the second connection flow channel 9, the siphon flow channel 4 and the liquid shunt flow channel 6 is not specifically limited and can be adjusted according to the needs of gene detection. For example, the width and depth of the siphon flow channel 4 can be in the range of 0.4mm-0.5mm. When the width and depth of the above-mentioned siphon flow channel 4 are in the range of 0.4mm-0.5mm, the blocking effect of the above-mentioned siphon flow channel 4 is better. For example, the width and depth of the first preset number of third connection flow channels 11, the first connection flow channel 2, the second connection flow channel 9 and the liquid shunt flow channel 6 can be about 1mm. For example, the width and depth of each reaction groove connection flow channel 13 in the second preset number of reaction groove connection flow channels 13 can be between 0.2mm-0.4mm. When the width and depth of the reaction groove connection flow channel 13 in the above-mentioned second preset number of reaction groove connection flow channels 13 are in the range of 0.2mm-0.4mm, the reaction groove connection flow channel 13 can achieve better blocking effect. The materials of the above-mentioned first preset number of third connection flow channels 11, the second preset number of reaction groove connection flow channels 13, the first connection flow channel 2, the second connection flow channel 9, the siphon flow channel 4 and the liquid shunt flow channel 6 can include but are not limited to: polymethyl methacrylate (PMMA), polycarbonate (PC), polystyrene (PS), polypropylene (PP) and glass. Here, the specific value of the above-mentioned second preset number is not limited. For example, the second preset number can be 4. It should be noted that the microfluidic closed microstructure for knee joint movement injury gene detection includes various flow channels for transmitting liquid.

[0032] In some embodiments, the plastic substrate 01 is provided with a sample injection groove 3, a liquid buffer chamber 5, a phase change material chamber 10, a second preset number of reaction grooves 14, and a waste liquid collection chamber 7. The sample injection groove 3 can be used to receive the sample reagent injected from the sample injection port 1. For example, 50 microliters of sample solution can be injected from the sample injection port 1 to the sample injection groove 3. The liquid buffer chamber 5 can be used to slow down the flow rate of the sample reagent flowing from the sample injection groove 3, thereby stabilizing the flow rate of the sample reagent. The phase change material chamber 10 can be used to receive the phase change material injected from the second connecting flow channel 9, and to store the phase change material and the cavity where the phase change material undergoes phase change. The phase change material is a material that can undergo phase change or other reversible phase change material. For example, the phase change material can be paraffin. The phase change material received by the phase change material chamber 10 can undergo phase change in the phase change material chamber 10. Each of the second preset number of reaction grooves 14 is provided with probes and primers for detecting the knee movement injury gene site. The sample reagent can be a blood sample. The waste liquid collection chamber 7 can be used to collect the waste liquid generated in the gene detection or to store the solution remaining after each of the second preset number of reaction grooves 14 is filled.

[0033] In some embodiments, the lower end of the sample injection groove 3 can be connected to the upper end of the liquid buffer chamber 5 through the siphon flow channel 4. The sample reagent in the sample injection groove 3 can be injected into the liquid buffer chamber 5 through the siphon flow channel 4. In use, the stage 02 can control the rotation speed and direction of the microfluidic closed microstructure for knee movement injury gene detection by program, so that the sample solution of the sample injection groove 3 flows into the liquid buffer chamber 5 through the siphon flow channel 4.

[0034] In some embodiments, the lower end of the liquid buffer chamber 5 can be connected to the waste liquid collection chamber 7 through the liquid diversion flow channel 6. One side of the liquid diversion flow channel 6 can be provided with a first preset number of chamber diversion ports. The other side of the liquid diversion flow channel 6 can be provided with a second preset number of groove diversion ports. The lower end of the phase change material chamber 10 can be connected to the first preset number of chamber diversion ports through the first preset number of third connecting flow channels 11. The second preset number of reaction grooves 14 can be connected to the second preset number of groove diversion ports through the second preset number of reaction groove connecting flow channels 13. Each of the second preset number of reaction groove connecting flow channels 13 can include at least one micro flow valve 12. Here, the specific value of the first preset number is not limited. For example, the first preset number can be 3. The micro flow valve 12 is movable under the drive of the phase change material.

[0035] In some embodiments, the phase change material chamber 10 includes a third preset number of microcavities. Each of the third preset number of microcavities contains phase change material. The phase change material contained in the third preset number of microcavities can control the closing of each microfluidic valve 12 contained in the second preset number of reaction groove connecting flow channels 13 by phase change. In use, the microfluidic closed microstructure for knee joint movement injury gene detection is placed behind the stage 02, and a micro-heater is arranged on the stage 02. The micro-heater is arranged directly below the phase change material chamber 10 and can heat the third preset number of microcavities in the phase change material chamber 10. In this regard, the specific value of the third preset number is not limited. For example, the third preset number can be 5. In use, after the phase change material in the phase change material chamber 10 is heated, the phase change material flows into each microfluidic valve contained in the second preset number of reaction groove connecting flow channels 13 by capillary action and the centrifugal force of the stage 02 after phase change, so as to fill each microfluidic valve, thereby converting each microfluidic valve to a closed state under the filling and solidification of the phase change material, and the phase change material can push the microfluidic valve to the inlet of the reaction groove to close the reaction groove. Due to the difference between the density of the solution in the reaction groove and the density of the phase change material and the small structure of the microfluidic closed microstructure for knee joint movement injury gene detection, the flow of the phase change material into the reaction groove is blocked. Therefore, the microfluidic valve and the reaction groove can be closed, and the microfluidic flow channel can be sealed. It should be noted that the purpose of converting each microfluidic valve to a closed state is to reduce the aerosol pollution of the environment and the pollution of other reaction zones during the PCR reaction of thermal cycling of the solution in the microfluidic closed microstructure for knee joint movement injury gene detection, and to reduce the loss of liquid in the reaction groove.

[0036] Optionally, as shown in Figure 1 the microfluidic closed microstructure for knee joint movement injury gene detection further includes a sample injection inlet 1, a first connecting flow channel 2, a phase change material injection inlet 8, and a second connecting flow channel 9. The sample injection inlet 1 can be used to inject a sample. The first connecting flow channel 2 can be used to connect the sample injection inlet 1 and the sample injection groove 3. The phase change material injection inlet 8 can be used to inject phase change material into the phase change material chamber 10. The second connecting flow channel 9 can be used to connect the phase change material chamber 10 and the phase change material injection inlet 8. In this regard, the diameters of the sample injection inlet 1 and the phase change material injection inlet 8 are not limited. For example, the diameters of the sample injection inlet 1 and the phase change material injection inlet 8 can be about 2 mm.

[0037] Optionally, as shown in Figure 1As shown, the upper end of the sample injection groove 3 is connected to the sample injection port 1 through the first connecting flow channel 2.

[0038] Optionally, as shown, Figure 1 As shown, the upper end of the phase change material chamber 10 is connected to the phase change material injection port 8 through the second connecting flow channel 9.

[0039] Optionally, as shown, Figure 1 As shown, the reaction groove 14 in the second preset number of reaction grooves 14 corresponds to the in-groove shunt port in the second preset number of in-groove shunt ports, and the reaction groove 14 in the second preset number of reaction grooves 14 and the corresponding in-groove shunt port are connected through a reaction groove connecting flow channel 13.

[0040] Optionally, the phase change material can be paraffin wax. Here, the specific type of phase change material is not limited.

[0041] Optionally, as shown, Figure 2 As shown, the plastic substrate 01 can be circular. Here, the shape of the plastic substrate 01 is not limited and can be adjusted according to experimental requirements.

[0042] Optionally, as shown, Figure 1 As shown, each micro flow valve 12 contained in the second preset number of reaction groove connecting flow channels 13 is semicircular. Here, the shape of each micro flow valve 12 contained in the second preset number of reaction groove connecting flow channels 13 is not limited and can be adjusted according to experimental requirements.

[0043] Optionally, as shown, Figure 1 As shown, the diameter of the sample injection port 1 can be about 2 mm.

[0044] Optionally, as shown, Figure 1 or Figure 2As shown, the shape of each of the second preset number of reaction grooves 14 can be circular. The phase change material contained in each of the third preset number of microcavities can change phase at a preset temperature. The at least one microfluidic valve 12 provided in each of the second preset number of reaction groove connecting flow channels 13 is made of an elastic material. The sample injection groove 3, the liquid buffer chamber 5, the phase change material chamber 10, the second preset number of reaction grooves 14, and the waste liquid collection chamber 7 are distributed in a diffusion shape with a preset center position 03. The sample injection groove 3 is located in the innermost circumferential area of the microfluidic closed microstructure for knee movement injury gene detection. The phase change material chamber 10 and the liquid buffer chamber 5 are located in the intermediate circumferential area of the microfluidic closed microstructure for knee movement injury gene detection. The second preset number of reaction grooves 14 and the waste liquid collection chamber 7 are located in the outermost circumferential area of the microfluidic closed microstructure for knee movement injury gene detection. The preset center position 03 can be hollow. The phase change material chamber 10 is provided around the second preset number of reaction grooves 14, and the distance between the phase change material chamber 10 and the second preset number of reaction grooves 14 is close. In use, the microfluidic closed microstructure for knee movement injury gene detection is installed on the carrier 02, and when the carrier 02 operates, the carrier 02 drives the microfluidic closed microstructure for knee movement injury gene detection to rotate clockwise or counterclockwise based on the preset center position 03. The shape of each of the second preset number of reaction grooves 14 is not specifically limited. The size of the reaction groove 14 is not specifically limited and can be set according to the specific detection scene. The phase change material contained in each of the third preset number of microcavities can change phase at a preset temperature. The specific temperature value of the preset temperature is not limited. The preset temperature is set according to the type of phase change material, for example, the phase change material is paraffin, and the preset temperature is the temperature at which paraffin changes phase. The at least one microfluidic valve 12 provided in each of the second preset number of reaction groove connecting flow channels 13 is made of an elastic material and has a semicircular shape. The microfluidic valve 12 can be made of rubber or polydimethylsiloxane (PDMS) material. The carrier 02 can be used to fix the microfluidic closed microstructure for knee movement injury gene detection and drive the microfluidic closed microstructure for knee movement injury gene detection to rotate clockwise or counterclockwise. The preset center position 03 of the microfluidic closed microstructure for knee movement injury gene detection can be used to connect with the carrier 02. The carrier 02 can be connected with a temperature control system and a motor. The rotation speed and direction of the carrier 02 are adjustable.The carrier 02 can include a tray 04. The tray 04 can include the micro-heater. The temperature control system can be used to control the micro-heater included in the tray 04 to heat the phase change material chamber 10 of the microfluidic closed microstructure for the knee motion injury gene detection. The motor of the carrier 02 can be arranged at the center of the carrier 02. In use, the motor speed and direction of the carrier 02 can be controlled to make the sample solution flow from the sample injection groove 3 to the liquid buffer chamber 5 through the siphon flow channel 4, make the sample solution in the liquid buffer chamber 5 inject into the second preset number of reaction grooves 14, and make the phase change material in the phase change material chamber 10 fill and solidify the microfluidic valve 12 in the second preset number of reaction groove connection flow channels 13, so that the microfluidic valve 12 is switched to a closed state, the second preset number of reaction grooves 14 are closed, the microfluidic closed microstructure for the knee motion injury gene detection is automatically detected, and the waste liquid flows into the waste liquid collection chamber 7. In use, when the microfluidic valve 12 is not driven and filled by the phase change material in the phase change material chamber 10, the microfluidic valve 12 is in an open state, when the phase change material in the phase change material chamber 10 is in a liquid state due to heating, the microfluidic valve 12 is switched to a closed state due to the filling and solidification of the liquid phase change material, so that the microfluidic valve 12 is in a closed state, and the microfluidic valve 12 can be automatically closed. The microfluidic valve 12 can be driven by the phase change material to move to the inlet of the reaction groove 14 to close the reaction groove 14, the density of the phase change material is different from the density of the solution in the reaction groove 14, and the structure of the microfluidic closed microstructure for the knee motion injury gene detection is small, which causes a large resistance to the phase change material flowing into the reaction groove 14, so that the phase change material does not flow into the reaction groove 14, and the reaction groove 14 is further closed.

[0045] The optional embodiment above is one of the inventive points of the embodiments of the present disclosure, and solves the technical problem of mechanical valve movement causing disturbance of liquid, long heating time of the microfluidic chip, long detection time, and deformation of the microfluidic chip. The factors of mechanical valve movement causing disturbance of liquid, long heating time of the microfluidic chip, long detection time, and deformation of the microfluidic chip are as follows: using a mechanical valve in the microfluidic chip, closing the microfluidic chip through the mechanical valve, or using a heat sealing technology to close the microfluidic chip, the movement of the mechanical valve will cause disturbance of liquid, and using the heat sealing technology to close the microfluidic chip will cause long heating time of the microfluidic chip, long detection time, and deformation of the microfluidic chip. If the above factors are solved, the disturbance of liquid caused by mechanical valve movement can be reduced, the heating time of the microfluidic chip can be shortened, the detection time can be shortened, and the deformation of the microfluidic chip can be reduced. In order to achieve this effect, the microfluidic valve provided in the microfluidic closed microstructure is made of an elastic material, and the closure of the provided microfluidic valve is achieved by phase change of a phase change material. Instead of using a mechanical valve or a heat sealing technology, the microfluidic closed microstructure is distributed outward from the preset center position 03, is a sealing structure, and can automatically mix and inject liquid into each chamber through the rotation speed and direction of the stage 02, reducing the operation difficulty and shortening the detection time. Therefore, the microfluidic valve in the microfluidic closed microstructure can reduce the disturbance of liquid, shorten the heating time of the microfluidic chip, further shorten the detection time, and reduce the deformation of the microfluidic chip.

[0046] Optionally, as Figure 1 or Figure 2As shown, one side of the liquid distribution channel 6 is provided with a target number of inlet groove distribution ports, which are used to connect with the target number of reaction grooves 14. The target number of reaction grooves 14 are standby reaction grooves. The target number of inlet groove distribution ports are externally covered by a covering assembly. The covering assembly is slidable. In the use state, the slidable covering assembly exposes the inlet groove distribution ports to further connect the inlet groove distribution ports with the reaction grooves 14. Sliding the covering assembly can cover the inlet groove corresponding to the damaged reaction groove. The sample injection port 1 of the microfluidic closed microstructure is provided with a piston. In the use state, the piston is pulled out to inject the sample solution. The target number of inlet groove distribution ports are used to connect with the target number of reaction grooves 14. The target number of reaction grooves 14 can be standby reaction grooves. The target number of inlet groove distribution ports are connected with the target number of reaction grooves 14 through the target number of reaction groove connection channels 13. The inlet groove distribution ports in the target number of inlet groove distribution ports are the same as the inlet groove distribution ports in the second preset number of inlet groove distribution ports. The reaction grooves in the target number of reaction grooves 14 are the same as the reaction grooves in the second preset number of reaction grooves 14. The reaction groove connection channels in the target number of reaction groove connection channels 13 are the same as the reaction groove connection channels in the second preset number of reaction groove connection channels 13. Therefore, the target number of reaction grooves 14, the target number of reaction groove connection channels 13, and the target number of inlet groove distribution ports will not be described again. One of the target number of inlet groove distribution ports is connected with one of the target number of reaction grooves 14 through one reaction groove connection channel 13. The covering assembly can be used to cover the target number of inlet groove distribution ports. In the use state, the slidable covering assembly exposes the inlet groove distribution ports to connect the reaction grooves 14. If the reaction grooves 14 connected with the microfluidic closed microstructure for knee joint movement injury gene detection are damaged, the slidable covering assembly can be used to cover the inlet groove distribution ports corresponding to the damaged reaction grooves 14, and a standby reaction groove 14 is installed at the reserved inlet groove distribution port for gene detection. For example, the covering assembly can be a sliding cover. The covering assembly (not shown in the figure) can slide left and right. The materials of the piston and the covering assembly can be the same as the material of the plastic substrate 01. When the microfluidic closed microstructure for knee joint movement injury gene detection is not used, the piston (not shown in the figure) can be used to plug the sample injection port 1 to reduce the pollution inside the microfluidic closed microstructure for knee joint movement injury gene detection.It should be noted that when sliding or adding the above-mentioned cover assembly, the cavities and flow channels in the microfluidic closed microstructure for knee joint movement injury gene detection can be detached from the above-mentioned plastic substrate 01 for sliding or adding. Each cavity and flow channel in the above-mentioned plastic substrate 01 can be detached. And the above-mentioned plastic substrate 01 has a reserved groove for installing a reserved reaction tank (not shown in the figure).

[0047] The optional embodiment above solves the technical problems of the pollution prone to occur in the microfluidic closed microstructure for knee joint movement injury gene detection placed, and the microfluidic closed microstructure for knee joint movement injury gene detection cannot be used again if the reaction tank is damaged, causing waste of the microfluidic closed microstructure for knee joint movement injury gene detection, and the microfluidic closed microstructure for knee joint movement injury gene detection is customized, and if the number of reaction tanks is not enough, it needs to be re-customized and prepared, causing poor flexibility of using the microfluidic closed microstructure for knee joint movement injury gene detection, as one of the inventive points of the embodiments of the present disclosure. The factors that cause the waste of the microfluidic closed microstructure for knee joint movement injury gene detection, the pollution prone to occur in the microfluidic closed microstructure for knee joint movement injury gene detection placed, and the poor flexibility of using the microfluidic closed microstructure for knee joint movement injury gene detection are as follows: the number of reaction tanks is fixed after the microfluidic closed microstructure for knee joint movement injury gene detection is prepared and completed, and the reaction tank cannot be added again, and if the reaction tank is damaged, the microfluidic closed microstructure for knee joint movement injury gene detection cannot be used for gene detection again, and the idle microfluidic closed microstructure for knee joint movement injury gene detection is prone to internal pollution. If the above factors are solved, the waste of the microfluidic closed microstructure for knee joint movement injury gene detection can be reduced, the internal pollution of the microfluidic closed microstructure for knee joint movement injury gene detection can be reduced, and the flexibility of using the microfluidic closed microstructure for knee joint movement injury gene detection can be improved. In order to achieve this effect, the target number of inlet groove distribution ports are arranged on one side of the liquid distribution channel 6, and the target number of inlet groove distribution ports are covered by the covering assembly. When it is necessary to add a reaction tank 14, the covering assembly is slid to add it. When the reaction tank 14 of the microfluidic closed microstructure for knee joint movement injury gene detection is damaged, the covering assembly can be slid to cover the inlet groove distribution port corresponding to the damaged reaction tank 14, so that the covered reaction tank 14 is not used in the detection process. The piston is installed at the sample inlet port 1 of the microfluidic closed microstructure for knee joint movement injury gene detection, so that the internal pollution of the microfluidic closed microstructure for knee joint movement injury gene detection in the idle state can be reduced. Therefore, the waste of the microfluidic closed microstructure for knee joint movement injury gene detection can be reduced, the internal pollution of the microfluidic closed microstructure for knee joint movement injury gene detection can be reduced, and the flexibility of using the microfluidic closed microstructure for knee joint movement injury gene detection can be improved.

[0048] Some embodiments of the present disclosure provide a microfluidic closed microstructure for knee motion injury gene detection, which can improve the sealing performance of the microfluidic chip and the reaction effect of the microfluidic chip, reduce the waste of sealing materials and the deformation of the microfluidic chip, and further shorten the time consumption of gene detection. Specifically, the reasons for poor sealing performance and poor reaction effect of the microfluidic chip, waste of sealing materials and deformation of the microfluidic chip, and long time consumption of gene detection are as follows: when the microfluidic chip detects genes by using mechanical valve sealing technology, the wear of mechanical parts causes the sealing performance of the microfluidic chip to be low, and the movement of the mechanical valve causes liquid disturbance in the microfluidic channel, resulting in poor reaction effect. When the microfluidic chip detects genes by using heat sealing technology, the time consumption of heating the sealing material is long, and the inaccuracy of temperature control causes the structure of the microfluidic chip to deform or the sealing material to be damaged, resulting in waste of sealing materials and long time consumption of gene detection. Based on this, some embodiments of the present disclosure provide a microfluidic closed microstructure for knee motion injury gene detection, which includes a plastic substrate, a first preset number of third connection flow channels, a second preset number of reaction groove connection flow channels, a siphon flow channel and a liquid shunt flow channel. The plastic substrate is provided with a sample injection groove, a liquid buffer chamber, a phase change material chamber, a second preset number of reaction grooves and a waste liquid collection chamber; the lower end of the sample injection groove and the upper end of the liquid buffer chamber are connected by the siphon flow channel; the lower end of the liquid buffer chamber and the waste liquid collection chamber are connected by the liquid shunt flow channel, one side of the liquid shunt flow channel is provided with a first preset number of chamber inlet shunt ports, the other side of the liquid shunt flow channel is provided with a second preset number of groove inlet shunt ports, the lower end of the phase change material chamber and the first preset number of chamber inlet shunt ports are connected by the first preset number of third connection flow channels, the second preset number of reaction grooves and the second preset number of groove inlet shunt ports are connected by the second preset number of reaction groove connection flow channels, and each reaction groove connection flow channel in the second preset number of reaction groove connection flow channels includes at least one microfluidic valve; the phase change material chamber includes a third preset number of microcavities, each microcavity in the third preset number of microcavities contains phase change material, and the phase change material contained in the third preset number of microcavities can control the closing of each microfluidic valve contained in the second preset number of reaction groove connection flow channels by phase change.In one aspect, instead of closing the microfluidic channel by using mechanical valve closing technology or using thermal sealing technology, after the phase change material is heated, the phase change material changes phase and flows into each microfluidic valve contained in the second preset number of reaction tank connecting flow channels by capillary action and centrifugal force to fill each microfluidic valve, so that the microfluidic valve is converted to a closed state under the filling and solidification of the phase change material, and the phase change material can push the microfluidic valve to the inlet of the reaction tank to close the reaction tank, and due to the difference between the density of the solution in the reaction tank and the density of the phase change material and the small structure of the microfluidic closed microstructure for knee joint movement injury gene detection, the flow of the phase change material into the reaction tank is hindered, so that the phase change material does not enter the reaction tank, thereby achieving automatic closing of the reaction tank and sealing of the microfluidic channel. In the working state, the microfluidic closed microstructure for knee joint movement injury gene detection is installed on the rotating stage, so that the phase change material can flow into each microfluidic valve contained in the second preset number of reaction tank connecting flow channels by centrifugal force. In another aspect, the microfluidic closed microstructure is a microstructure, so the microfluidic closed microstructure has a large surface area to volume ratio, can quickly conduct and dissipate heat, so that the phase change material quickly reaches and maintains the melting point, thereby reducing the time spent on heating the phase change material and the time spent on gene detection, thereby reducing the waste of microfluidic chip materials and the damage to the microfluidic chip structure. In another aspect, the microfluidic closed microstructure includes a sample injection tank, a liquid buffer chamber, a phase change material chamber, the second preset number of reaction tanks and a waste liquid collection chamber, so that the integrated design of the microfluidic closed microstructure can simplify the steps of gene detection, thereby further shortening the time spent on gene detection and improving the accuracy of gene detection. Therefore, the microfluidic closed microstructure can improve the sealing performance, shorten the time spent on gene detection, improve the accuracy of gene detection, and reduce the waste of phase change material and reduce the deformation of the microfluidic closed microstructure.

[0049] The above description is only some of the preferred embodiments of the present disclosure and an explanation of the principles of the technology used. Those skilled in the art should understand that the scope of the invention involved in the embodiments of the present disclosure is not limited to the technical solutions formed by the specific combinations of the above technical features, and should also cover other technical solutions formed by any combination of the above technical features or equivalent features without departing from the above inventive concept. For example, the above features are replaced with each other to form a technical solution with similar functions disclosed in the embodiments of the present disclosure (but not limited to).

Claims

1. A microfluidic closed microstructure for detecting a knee joint motion injury gene, characterized in that, The microfluidic closed microstructure comprises a plastic substrate, a first preset number of third connecting flow channels, a second preset number of reaction tank connecting flow channels, a siphon flow channel and a liquid shunt flow channel, wherein The plastic substrate is provided with a sample injection groove, a liquid buffer chamber, a phase change material chamber, the second preset number of reaction tanks and a waste liquid collection chamber; The lower end of the sample injection groove is connected to the upper end of the liquid buffer chamber through the siphon flow channel; The lower end of the liquid buffer chamber is connected to the waste liquid collection chamber through the liquid shunt flow channel, one side of the liquid shunt flow channel is provided with a first preset number of chamber shunt inlets, the other side of the liquid shunt flow channel is provided with a second preset number of tank shunt inlets, the lower end of the phase change material chamber is connected to the first preset number of chamber shunt inlets through the first preset number of third connecting flow channels, the second preset number of reaction tanks are connected to the second preset number of tank shunt inlets through the second preset number of reaction tank connecting flow channels, and each of the second preset number of reaction tank connecting flow channels comprises at least one microflow valve. The phase change material chamber comprises a third preset number of microcavities, wherein each of the third preset number of microcavities contains phase change material, and the phase change material contained in the third preset number of microcavities can control the closing of each microflow valve contained in the second preset number of reaction tank connecting flow channels through phase change.

2. The microfluidic closed microstructure for detecting the knee joint movement injury gene according to claim 1, wherein, The microfluidic closed microstructure for detecting knee joint movement injury genes further comprises a sample injection port, a first connecting flow channel, a phase change material injection port and a second connecting flow channel.

3. The microfluidic closed microstructure for detecting the knee joint movement injury gene according to claim 2, characterized in that, The upper end of the sample injection groove is connected to the sample injection port through the first connecting flow channel.

4. The microfluidic closed microstructure for detecting the knee joint movement injury gene according to claim 2, wherein, The upper end of the phase change material chamber is connected to the phase change material injection port through the second connecting flow channel.

5. The microfluidic closed microstructure for detecting the knee joint movement injury gene according to claim 1, wherein, Each of the second preset number of reaction tanks corresponds to one of the second preset number of tank shunt inlets, and each of the second preset number of reaction tanks and the corresponding tank shunt inlet are connected through a reaction tank connecting flow channel.

6. The microfluidic closed microstructure for detecting a knee joint motion injury gene according to claim 1, wherein, The phase change material is paraffin.

7. The microfluidic closed microstructure for detecting the knee joint movement injury gene according to claim 1, wherein, The plastic substrate is circular.

8. The microfluidic closed microstructure for detecting the knee joint movement injury gene according to claim 1, wherein, Each of the microflow valves contained in the second preset number of reaction tank connecting flow channels is semicircular.

9. The microfluidic closed microstructure for detecting the knee joint movement injury gene according to claim 2, wherein, The diameter of the sample injection port is 2mm.

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