Batch liquid adding and changing device for cell culture

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell culture batch liquid adding and changing device which comprises a base, a support is fixedly installed on the upper side of the base, sliding grooves are formed in the left end and the right end of the support respectively, sliding blocks are installed in the two sliding grooves in a sliding mode respectively, and a bidirectional electric telescopic rod is fixedly installed in the support. The left end and the right end of the bidirectional electric telescopic rod are connected with two sets of sliding blocks, second electric telescopic rods are installed in the lower ends of the two sets of sliding blocks, and multi-channel pipettes are fixedly installed at the lower ends of the two sets of second electric telescopic rods. According to the utility model, a plurality of culture containers are inserted into a plurality of sockets on a placement rack on the right side of a mounting plate; a second electric telescopic rod in a sliding block on the right side of a base drives a multi-channel pipettor and a plurality of groups of pipettes to move downwards to be inserted into the plurality of groups of culture containers; therefore, the culture solution in the multiple groups of culture containers inserted into the placement rack on the right side of the mounting plate can be extracted.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture technical field, concretely is a cell culture batch liquid adding and changing device. BACKGROUND

[0002] Cell culture constitutes the basis of cell biology research. With the continuous division and metabolic activity of cells, in order to ensure the normal growth of cells, the culture medium must be added and replaced regularly, and this process includes cell liquid adding and changing. The traditional cell changing step includes: first, using a pipette to suck the old culture medium, and then adding new culture medium into the culture container. However, when handling a large number of culture containers, the technician has to repeatedly perform pipetting operations in the clean bench, which not only is inefficient and cumbersome, but also may increase the risk of cell contamination. Therefore, we propose a cell culture batch liquid adding and changing device to solve the above problems. SUMMARY

[0003] The utility model aims at providing a cell culture batch liquid adding and changing device to solve the problem of having to repeatedly perform pipetting operations in the clean bench when handling a large number of culture containers, which not only is inefficient and cumbersome, but also may increase the risk of cell contamination.

[0004] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a cell culture batch liquid adding and changing device, comprising a base, a bracket is fixedly installed on the upper side of the base, a sliding slot is formed in the left and right ends of the bracket, a sliding block is slidingly installed in each of the two groups of sliding slots, a bidirectional electric telescopic rod is fixedly installed in the bracket, the left and right ends of the bidirectional electric telescopic rod are connected with the two groups of sliding blocks, a second electric telescopic rod is installed in the lower end of each of the two groups of sliding blocks, a multi-channel pipette is fixedly installed at the lower end of each of the two groups of second electric telescopic rods, a plurality of pipettes are movably installed at the lower end of the multi-channel pipette, a rotating assembly is arranged on the base, a locking assembly is arranged in the left end of the base, and the locking assembly is connected with the rotating assembly.

[0005] Preferably, the two groups of sliding slots are symmetrically arranged left and right, and the opposite sides of the two groups of sliding blocks are spaced apart from the inner walls of the left and right sides of the bracket by the same distance.

[0006] Preferably, the rotating assembly comprises a rotating shaft, the rotating shaft is rotatably installed in the base, an installation plate is fixedly installed at the upper end of the rotating shaft, a placing rack is fixedly installed at the left and right ends of the installation plate, a plurality of sockets are arranged in each of the two groups of placing racks, and the number of the plurality of sockets in each of the two groups of placing racks is consistent with the number of the plurality of pipettes at the lower end of each of the two groups of multi-channel pipettes.

[0007] Preferably, the two groups of racks are arranged on the lower side of the two groups of multi-channel pipettes, and the two groups of racks and the two groups of multi-channel pipettes are arranged in parallel.

[0008] Preferably, the locking assembly comprises a first movable slot arranged in the left end of the base, a spring arranged in the first movable slot, a limiting block movably arranged at the opening of the first movable slot, limiting grooves arranged at the left and right ends of the mounting plate, a second movable slot arranged in the left end of the base, and a pressing plate fixedly arranged at the lower left end of the limiting block and sliding in the second movable slot.

[0009] Preferably, the lower end of each of the two groups of limiting slots and the upper end of the limiting block are arc-shaped, and the inner wall diameter of each of the two groups of limiting slots is consistent with the upper end diameter of the limiting block.

[0010] Preferably, the spacing between the rotating shaft and the limiting grooves at the left and right ends of the mounting plate is consistent, and the rotating shaft is arranged at the lower center of the mounting plate.

[0011] Preferably, the inner wall of each of the plurality of sockets on the two groups of racks is provided with a protective pad made of rubber.

[0012] Compared with the prior art, the device has the advantages that: the second electric telescopic rod in the slider on the right side of the base drives the multi-channel pipette and the plurality of pipettes to move downward and insert into the plurality of culture containers, so that the culture solution in the plurality of culture containers inserted into the rack on the right side of the mounting plate can be extracted, then the mounting plate and the two groups of racks are rotated, the extracted culture containers are moved to the left side of the base, and then the second electric telescopic rod in the slider on the left side of the base drives the multi-channel pipette and the plurality of pipettes to move downward and insert into the plurality of culture containers, so as to add new culture solution to the culture containers, which not only improves the convenience and safety of operation, but also avoids the repetition of the liquid adding and replacing process, and these advantages make the device have a wide application prospect in the field of cell culture, especially in the case of a large number of culture containers required for experiments, which can significantly improve the work efficiency and the reliability of experimental results. BRIEF DESCRIPTION OF DRAWINGS

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0014] Figure 1It is a structure front view schematic diagram of the utility model.

[0015] Figure 2 It is a structure front view schematic diagram of the utility model.

[0016] Figure 3 It is a structure left view schematic diagram of the utility model.

[0017] Figure 4 It is a structure left view schematic diagram of the utility model Figure 2 It is a structure left view schematic diagram of the utility model.

[0018] In the figure: 1, base; 2, support; 3, sliding groove; 4, sliding block; 5, two-way electric telescopic rod; 6, second electric telescopic rod; 7, multi-channel pipettor; 8, pipette; 9, rotating shaft; 10, mounting plate; 11, placing rack; 12, socket; 13, first movable slot; 14, spring; 15, limiting block; 16, limiting slot; 17, second movable slot; 18, pressing plate. Specific implementation

[0019] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0020] Please refer to Figures 1-4 The utility model provides an embodiment: a cell culture batch liquid adding and replacing device, including base 1, the upside fixed mounting of base 1 has support 2, the left and right two ends in support 2 are all set up sliding groove 3, two groups of sliding groove 3 are all slidingly installed with sliding block 4, the fixed mounting of two-way electric telescopic rod 5 in support 2, the left and right two ends of two-way electric telescopic rod 5 are connected with two groups of sliding block 4, the lower end in two groups of sliding block 4 is all installed with second electric telescopic rod 6, the lower end of two groups of second electric telescopic rod 6 is all fixedly installed with multi-channel pipettor 7, the lower end of multi-channel pipettor 7 is movably installed with multiple pipettes 8, and rotating assembly is arranged on base 1, locking assembly is arranged in the left end of base 1, and the locking assembly is connected with the rotating assembly, the device realizes the accurate control to the culture container through the collaborative work of rotating assembly and locking assembly. Specifically, the rotating assembly can drive base 1 to rotate around its axis, so that the cell culture container can be uniformly filled or replaced by two groups of multi-channel pipettors 7 and multiple pipettes 8. And the locking assembly ensures the stable fixation of the rotating assembly at a specific angle, prevents the position change of the cell culture container caused by rotation during liquid adding and replacing, and guarantees the accuracy and safety of operation.

[0021] Further, the two groups of sliding grooves 3 are symmetrically arranged left and right, and the opposite sides of the two groups of sliding blocks 4 are arranged at the same distance from the inner walls of the left and right sides of the support 2. This structure ensures that the culture container can uniformly receive the filling or replacement of the culture solution during the filling and replacement process, avoiding the problem of inaccurate liquid filling or replacement due to inconsistent spacing.

[0022] Further, the rotating assembly includes a rotating shaft 9 rotatably installed in the base 1, and the upper end of the rotating shaft 9 is fixedly installed with a mounting plate 10. The left and right ends of the mounting plate 10 are each fixedly installed with a placement rack 11. A plurality of sockets 12 are arranged in the two groups of placement racks 11. The number of the plurality of sockets 12 in the two groups of placement racks 11 is consistent with the number of the plurality of pipettes 8 at the lower end of the two groups of multichannel pipettors 7. This structure rotates the two groups of placement racks 11 and the mounting plate 10 about the rotating shaft 9, thereby changing the positions of the placement racks 11 at the left and right ends of the mounting plate 10, facilitating the filling and replacement of the culture container.

[0023] Further, the two groups of placement racks 11 are located below the two groups of multichannel pipettors 7, and the two groups of placement racks 11 and the two groups of multichannel pipettors 7 are arranged in parallel. This structure allows the culture container to more stably receive the filling or replacement of the culture solution during the filling and replacement process, thereby improving the stability and efficiency of the operation. In addition, due to the parallel arrangement of the placement racks 11 and the multichannel pipettors 7, the culture solution can be accurately filled or replaced.

[0024] Further, the locking assembly includes a first movable slot 13 formed in the left end of the base 1, a spring 14 installed in the first movable slot 13, a limiting block 15 movably installed at the opening of the first movable slot 13, a limiting slot 16 formed at the left and right ends of the mounting plate 10, a second movable slot 17 formed in the left end of the base 1, and a pressing plate 18 fixedly installed at the lower left end of the limiting block 15 and sliding in the second movable slot 17. When the mounting plate 10 and the two groups of placement racks 11 are rotated, the pressing plate 18 drives the limiting block 15 to slide out of the limiting slot 16 at the left end of the mounting plate 10, thereby rotating the mounting plate 10 and the two groups of placement racks 11. Conversely, the limiting block 15 is inserted into the limiting slot 16, thereby fixing the mounting plate 10 and the two groups of placement racks 11, ensuring that the rotating assembly does not accidentally rotate during the filling and replacement process, thereby ensuring the safety and accuracy of the operation.

[0025] Further, the lower end openings of the two sets of limiting grooves 16 and the upper end edges of the limiting blocks 15 are arc-shaped, and the inner wall diameters of the two sets of limiting grooves 16 are consistent with the upper end diameters of the limiting blocks 15. This structure enables the limiting blocks 15 to smoothly slide into or out of the limiting grooves 16, thereby achieving locking and unlocking of the rotating assembly. This design not only simplifies the operation process, but also reduces wear and tear on the assembly due to the smooth transition of the arc-shaped edges, thereby prolonging the service life of the device.

[0026] Further, the spacing between the rotating shaft 9 and the limiting grooves 16 at the left and right ends of the mounting plate 10 is consistent, and the rotating shaft 9 is located at the lower center of the mounting plate 10. This structure ensures that the limiting grooves 16 at the left and right ends of the mounting plate 10 remain in the same position on the left and right sides of the base 1 when the mounting plate 10 rotates on the rotating shaft 9.

[0027] Further, the inner walls of the multiple sets of sockets 12 on the two sets of placement racks 11 are provided with protective pads made of rubber. This structure can prevent the culture containers inserted into the multiple sets of sockets 12 from shaking when the mounting plate 10 and the two sets of placement racks 11 rotate, so that the culture containers fit tightly with the inner walls of the multiple sets of sockets 12, thereby protecting the culture containers from shaking and breaking.

[0028] Working principle: When adding liquid and replacing liquid in the culture containers, Figure 2 , Figure 3 and Figure 4As shown, first, the multiple sets of culture containers are inserted into the multiple sets of sockets 12 of the multiple sets of racks 11 at the right end of the mounting plate 10, the second electric telescopic rod 6 in the slider 4 at the right end of the bracket 2 drives the multichannel pipettor 7 and the multiple sets of pipettes 8 to move downward, then the multiple sets of pipettes 8 are inserted into the multiple sets of culture containers to extract the culture solution, then the second electric telescopic rod 6 drives the multichannel pipettor 7 and the multiple sets of pipettes 8 to slide back to the original position, the right end of the telescopic end of the bidirectional electric telescopic rod 5 drives the slider 4 to slide in the sliding groove 3 at the right end of the bracket 2, and the multichannel pipettor 7 and the multiple sets of pipettes 8 move to the right end of the bracket 2 with the slider 4, then the culture solution extracted from the multichannel pipettor 7 and the multiple sets of pipettes 8 is discharged, then the right end of the telescopic end of the bidirectional electric telescopic rod 5 drives the slider 4 to slide in the sliding groove 3 at the right end of the bracket 2, and the multichannel pipettor 7 and the multiple sets of pipettes 8 move back to the original position with the slider 4, the pressing plate 18 is pressed, the pressing plate 18 drives the limiting block 15 to slide in the first movable groove 13 and the second movable groove 17, the limiting block 15 slides into the first movable groove 13 and slides out of the limiting groove 16 at the left end of the mounting plate 10, then the mounting plate 10 and the two sets of racks 11 are rotated, and the rack 11 at the right end of the mounting plate 10 moves to the left end of the base 1, then the second electric telescopic rod 6 in the slider 4 at the left end of the bracket 2 drives the multichannel pipettor 7 and the multiple sets of pipettes 8 to move downward, then the multiple sets of pipettes 8 are inserted into the multiple sets of culture containers to add culture to the multiple sets of culture containers, and the rack 11 at the left end of the mounting plate 10 moves to the right end of the base 1, a new set of culture containers is inserted into the multiple sets of sockets 12 on the rack 11, and the above operation is repeated, so that a large number of culture containers can be added with liquid and exchanged with liquid, and the above is the whole working principle of the utility model.

[0029] It is apparent for those skilled in the art that the utility model is not limited to the details of the above-mentioned exemplary embodiments, and can be realized in other specific forms without departing from the spirit or basic features of the utility model. Therefore, the embodiments should be regarded as exemplary and non-limiting from any point of view, and the scope of the utility model is defined by the appended claims rather than the above description, and therefore all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the utility model. Any reference signs in the claims should not be regarded as limiting the involved claims.

Claims

1. A cell culture batch liquid exchange device, comprising a base (1), characterized in that: The upper side of the base (1) is fixedly installed with a support (2), the left and right ends of the support (2) are both provided with a sliding groove (3), two groups of sliding grooves (3) are both slidably installed with a sliding block (4), the support (2) is fixedly installed with a two-way electric telescopic rod (5), the left and right ends of the two-way electric telescopic rod (5) are connected with two groups of sliding blocks (4), the lower ends of two groups of sliding blocks (4) are both installed with a second electric telescopic rod (6), the lower ends of two groups of second electric telescopic rods (6) are both fixedly installed with a multi-channel pipettor (7), the lower end of the multi-channel pipettor (7) is movably installed with a plurality of pipettes (8), a rotating assembly is arranged on the base (1), a locking assembly is arranged in the left end of the base (1), and the locking assembly is connected with the rotating assembly.

2. The cell culture batch liquid exchange device according to claim 1, wherein: The two groups of sliding grooves (3) are symmetrically arranged, and the opposite sides of the two groups of sliding blocks (4) are spaced apart from the inner walls of the left and right sides of the support (2) by the same distance.

3. The cell culture batch liquid exchange device according to claim 1, wherein: The rotating assembly comprises a rotating shaft (9), the rotating shaft (9) is rotatably installed in the base (1), the upper end of the rotating shaft (9) is fixedly installed with a mounting plate (10), the left and right ends of the mounting plate (10) are both fixedly installed with a placing rack (11), a plurality of sockets (12) are arranged in two groups of placing racks (11), and the number of the plurality of sockets (12) in the two groups of placing racks (11) is consistent with the number of the plurality of pipettes (8) at the lower end of the two groups of multi-channel pipettors (7).

4. The cell culture batch liquid exchange device according to claim 3, wherein: The two groups of placing racks (11) are below the two groups of multi-channel pipettors (7), and the two groups of placing racks (11) and the two groups of multi-channel pipettors (7) are arranged in parallel.

5. The cell culture batch liquid exchange device according to claim 3, wherein: The locking assembly comprises a first movable slot (13), the first movable slot (13) is arranged in the left end of the base (1), the first movable slot (13) is installed with a spring (14), the opening of the first movable slot (13) is movably installed with a limiting block (15), the left and right ends of the mounting plate (10) are both provided with a limiting slot (16), the left end of the base (1) is provided with a second movable slot (17), the lower left side of the limiting block (15) is fixedly installed with a pressing plate (18), and the pressing plate (18) slides in the second movable slot (17).

6. The cell culture batch liquid exchange device according to claim 5, wherein: The lower end openings of the two groups of limiting slots (16) and the upper end edges of the limiting blocks (15) are both arc-shaped, and the diameters of the inner walls of the two groups of limiting slots (16) are consistent with the diameters of the upper ends of the limiting blocks (15).

7. The cell culture batch liquid exchange device according to claim 3, wherein: The distance between the rotating shaft (9) and the limiting slots (16) at the left and right ends of the mounting plate (10) is consistent, and the rotating shaft (9) is located at the lower center of the mounting plate (10).

8. The cell culture batch liquid exchange device according to claim 3, wherein: The inner walls of the plurality of sockets (12) on the two groups of placing racks (11) are provided with rubber protective pads.

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