Bionic flora parallel fermentation system
By using a parallel fermentation system with multiple fermenters, the problem of unstable fermentation ratio of biomimetic microbial communities was solved, achieving efficient preparation and quality stability of biomimetic microbial communities. The method involves parallel fermentation with multiple fermenters and proportional mixing followed by centrifugal freeze-drying.
Patent Information
- Application Number
- CN202422986049.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-04
- Publication Date
- 2025-12-26
- Estimated Expiration
- 2034-12-04
AI Technical Summary
Existing technologies struggle to achieve stable proportions and structures in biomimetic microbial community fermentation, while single-strain fermentation suffers from problems due to the excessively large number of microorganisms and the difficulty in achieving it.
A biomimetic microbial community was prepared by using a parallel fermentation system with multiple fermenters to ferment each strain separately, then mixing them in the required proportions and centrifuging and freeze-drying them.
It achieves stable proportions and convenient preparation of biomimetic microbial communities, avoids the problem of excessive quantities in single-strain fermentation, and improves fermentation efficiency and product quality stability.
Smart Images
Figure CN223723118U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of bionic bacterial population fermentation processing and production technology, and particularly relates to a bionic bacterial population parallel fermentation system. BACKGROUND
[0002] Human microecology is highly related to human health. After a long biological evolution process, normal bacterial population is in a symbiotic state with human body and establishes a close relationship with human body, which plays a very important role in promoting the perfection of human physiological functions. They have formed a unity of interdependence, mutual benefit, mutual coordination and mutual restraint. At present, human microecological intervention has developed from the initial dietary intervention, supplement of probiotics and prebiotics intervention to the present fecal bacteria transplantation intervention. Fecal bacteria transplantation, as a way of replacing the whole human intestinal flora, has been widely used in clinical effect. However, fecal bacteria transplantation has disadvantages such as unstable donor bacterial population and trace risk factors of disease treatment. In order to solve the disadvantages of natural fecal bacteria donors, artificial bionic bacterial population has obvious advantages. That is, after screening and culturing of beneficial bacteria in human body, the bacterial population is compounded according to the structure of healthy human bacterial population to serve as a donor for bacterial population replacement, so as to realize the stability of the quality of the donor bacterial population and avoid the risk of disease treatment. However, as the bionic bacterial population is composed of dozens to hundreds of bacteria, it is difficult to obtain a bacterial population with stable proportion structure by mixed fermentation. Single bacterial fermentation has the problem of being too large in quantity and difficult to realize. SUMMARY
[0003] The bionic bacterial population parallel fermentation system provided by the utility model is connected in parallel by multiple fermentation tanks, and multiple bacterial species are simultaneously fermented at one time, and then multiple bacterial species are mixed and centrifuged to prepare the bionic bacterial population.
[0004] The bionic bacterial population parallel fermentation system comprises a fermentation tank, a fermentation tank interlayer, a fermentation tank discharge pipeline, an exhaust pipeline, an interlayer water outlet pipeline, a steam pipeline and an interlayer water inlet pipeline. The fermentation tank is arranged in the fermentation tank interlayer, and a stirring motor is fixed to the top end of the fermentation tank interlayer. The stirring motor is connected to stirring blades through a stirring shaft. The tank body is connected to the steam pipeline and the exhaust pipeline. The tank body is provided with a fermentation tank steam valve and a fermentation exhaust valve. The bottom of the tank body is connected to the fermentation tank discharge pipeline, and the tank body is provided with a fermentation tank discharge valve. The fermentation tank interlayer is connected to the interlayer water inlet pipeline and provided with an interlayer inlet valve. The fermentation tank interlayer is also connected to the interlayer water outlet pipeline and provided with an interlayer outlet valve.
[0005] The steam pipeline is connected to the fermentation tank discharge pipeline, and the steam pipeline is provided with a fermentation tank discharge pipeline steam valve and a parallel fermentation tank discharge valve.
[0006] The stirring shaft side wall is provided with multiple stirring blades, and the number of the stirring blades is three.
[0007] The fermentation tanks are in parallel fermentation at least three.
[0008] The utility model has compared with prior art has the advantage that: the utility model discloses a bionic bacterial population fermentation system first carries out fermentation to each bacterial strain of probiotic bacteria in fermentation tank respectively, so that it can obtain good growth, then mixes and centrifugal freeze-drying after the proportion required, can conveniently obtain the bionic bacterial population that imitates human body beneficial bacterial population structure. BRIEF DESCRIPTION OF DRAWINGS
[0009] Figure 1 It is a structure schematic diagram of the utility model discloses a bionic bacterial population parallel fermentation system.
[0010] As shown in the figure: 1, fermentation tank, 2, fermentation tank interlayer, 3, fermentation tank steam valve, 4, fermentation tank exhaust valve, 6, interlayer outlet valve, 7, interlayer import valve, 8, fermentation tank discharge pipeline, 9, fermentation tank discharge valve, 10, fermentation tank discharge pipeline steam valve, 11, exhaust pipeline, 12, interlayer outlet pipeline, 13, steam pipeline, 14, interlayer inlet pipeline, 15, parallel fermentation tank discharge valve, 101, stirring shaft, 102, stirring motor, 103, stirring paddle. DETAILED DESCRIPTION
[0011] The utility model will be further explained in detail in combination with the drawings.
[0012] Combination Figure 1 A bionic bacterial population parallel fermentation system, including fermentation tank 1, fermentation tank interlayer 2, fermentation tank discharge pipeline 8, exhaust pipeline 11, interlayer outlet pipeline 12, steam pipeline 13 and interlayer inlet pipeline 14, the fermentation tank 1 is placed in fermentation tank interlayer 2, and the top is fixed with stirring motor 102, is connected with stirring paddle 103 through stirring shaft 101, and the tank body is connected with steam pipeline 13 and exhaust pipeline 11, is equipped with fermentation tank steam valve 3 and fermentation exhaust valve 4 respectively, is connected with fermentation tank discharge pipeline 8 in the bottom, is equipped with fermentation tank discharge valve 9, the fermentation tank interlayer 2 is connected with interlayer inlet pipeline 14, is equipped with interlayer import valve 7, is connected with interlayer outlet pipeline 12, and is equipped with interlayer outlet valve 6.
[0013] The steam pipeline 13 is connected with the fermentation pipe discharge pipeline 8, is equipped with fermentation tank discharge pipeline steam valve 10 and parallel fermentation tank discharge valve 15.
[0014] The multiple stirring paddles 103 of the stirring shaft 101 side wall, the number of the stirring paddle is three groups.
[0015] The fermentation tank 1 is in parallel fermentation at least three.
[0016] The utility model discloses when implementing, three strains are fermented in three fermenting tanks respectively, the steam valve 3 and fermentation exhaust valve 4 of opening fermenting tank release steam into fermenting tank 1, and temperature reaches 115 DEG C, keeps 30 minutes after, closes fermenting tank steam valve 3, opens interlayer import valve 7 and interlayer export valve 6, and the constant temperature water of 37 DEG C-39 DEG C is passed in, and the strain is connected when fermenting tank 1 is cooled to 39 DEG C, keeps the temperature of the fermentation broth in fermenting tank 1 for 37 DEG C-39 DEG C, opens stirring motor 102, and keeps the speed for 15-20 every minute, carries out constant temperature fermentation, and after fermenting 8-12 hours, when the fermentation broth OD600 value reaches 1.5, the interlayer constant temperature water temperature is reduced to 10 degrees Celsius, and according to OD600 value, each fermenting tank bacteria liquid discharge speed is calculated, so that the proportion of each bacteria in the final mixed fermentation broth is the required proportion. Opening fermenting tank discharge pipeline steam valve 10 and parallel fermenting tank discharge valve 15, steam sterilization is carried out to fermenting tank discharge pipeline 8 for 15 minutes, and then fermenting tank discharge pipeline steam valve 10 is closed, fermenting tank discharge valve 9 is opened, and the fermentation broth in each fermenting tank is evenly discharged from fermenting tank discharge pipeline 8, and then after the multi-strain biomimetic microbial population is harvested by centrifuge, it can be freeze-dried.
Claims
1. A biomimetic parallel fermentation system for microbial communities, characterized in that, The fermentation tank includes a fermentation tank (1), a fermentation tank jacket (2), a fermentation tank discharge pipe (8), an exhaust pipe (11), a jacket water outlet pipe (12), a steam pipe (13), and a jacket water inlet pipe (14). The fermentation tank (1) is placed inside the fermentation tank jacket (2). A stirring motor (102) is fixed at the top and connected to a stirring blade (103) via a stirring shaft (101). The tank body is connected to the steam pipe (13) and the exhaust pipe (11), and is equipped with a fermentation tank steam valve (3) and a fermentation exhaust valve (4), respectively. The bottom is connected to the fermentation tank discharge pipe (8) and is equipped with a fermentation tank discharge valve (9). The fermentation tank jacket (2) is connected to the jacket water inlet pipe (14) and is equipped with a jacket inlet valve (7). It is connected to the jacket water outlet pipe (12) and is equipped with a jacket outlet valve (6).
2. The biomimetic microbial community parallel fermentation system according to claim 1, characterized in that, The steam pipe (13) is connected to the fermentation pipe discharge pipe (8), and is equipped with a steam valve (10) for the fermentation tank discharge pipe and a parallel fermentation tank discharge valve (15).
3. The biomimetic microbial community parallel fermentation system according to claim 1, characterized in that, The fermentation tanks (1) mentioned above are in parallel fermentation at least three times.