Reaction device for polypeptide synthesis
By introducing electric heating tubes and cleaning components into the peptide synthesis reaction device, the problem of residue removal was solved, temperature control and cleaning functions were realized, equipment performance and synthesis efficiency were improved, and service life was extended.
Patent Information
- Application Number
- CN202520054828.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-10
- Publication Date
- 2025-12-30
- Estimated Expiration
- 2035-01-10
AI Technical Summary
Existing peptide synthesis reaction equipment lacks self-cleaning capabilities, resulting in reagent and byproduct residues that affect equipment performance and lifespan, increase maintenance costs, and reduce synthesis efficiency and quality.
A reaction apparatus for peptide synthesis was designed, equipped with an electric heating tube and a cleaning assembly. The temperature of the reactor is regulated by the electric heating tube and the air is blown by a pump. The residue is removed by the cleaning assembly using a brush and water flow.
It achieves precise control of reactor temperature, ensuring that the temperature inside the reactor is within the optimal range, and can efficiently and thoroughly remove residual reagents and by-products, extending equipment life, maintaining equipment performance, and improving synthesis efficiency and quality.
Smart Images
Figure CN223732776U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to polypeptide synthesis technical field, specifically a polypeptide synthesis reaction device. BACKGROUND
[0002] The process of polypeptide synthesis is the condensation reaction of amino acids, and the peptide chain of different amino acids is synthesized. The impurities in the reagent can affect the smoothness of the synthesis instrument liquid pipe and the effective closure of the electromagnetic valve, causing the mixing of amino acids and solvents, affecting the purity of polypeptides or the change of long peptide chains.
[0003] Some existing polypeptide synthesis reaction devices adopt a rotating reaction tank mode, which promotes the catalytic reaction of polypeptides by shaking to achieve the purpose of synthesis. However, in actual operation, such reaction devices generally have a defect, i.e., they are not equipped with a self-cleaning function. This makes it difficult to effectively remove the residual reagents in the tank and the by-products generated during the synthesis process. If these residues are not timely treated, they will gradually accumulate inside the equipment, which not only greatly reduces the performance of the equipment, but also is likely to cause equipment failure, shortens the service life of the equipment, increases maintenance costs and downtime, and negatively affects the efficiency and quality of polypeptide synthesis.
[0004] Therefore, the utility model provides a polypeptide synthesis reaction device to solve the above problems. UTILITY MODEL CONTENTS
[0005] (I) Technical problems solved
[0006] The utility model provides a polypeptide synthesis reaction device, aiming to solve the problems raised in the background art.
[0007] (II) Technical solutions
[0008] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a heat preservation box, a wear-resistant outer tube fixedly installed on the inner top wall of the heat preservation box, a stepped end provided at the inner arc surface top end of the wear-resistant outer tube, an air outlet provided at the outer arc surface side edge of the wear-resistant outer tube, an air inlet provided at the lower surface middle part of the wear-resistant outer tube, a heating plate sleeved on the inner arc surface of the wear-resistant outer tube, the heating plate being attached to the inner arc surface of the wear-resistant outer tube, a heat-conducting plate placed on the lower surface of the heating plate, the surface of the heat-conducting plate being close to the air inlet, the air inlet and the air outlet being placed vertically, a reaction tube movably sleeved on the stepped end of the top of the wear-resistant outer tube, a bent end provided at the inner arc surface bottom of the reaction tube, an inner container movably sleeved on the upper surface of the bent end, and an arc-shaped end provided on the upper surface of the inner container.
[0009] As a preferred technical scheme of the present application, the arc-shaped end is arranged on the surface of the bent end, one end of the inner container is connected to the inside of the wear-resistant outer tube, and the electric heating pipe is arranged at the gap between the bent end and the wear-resistant outer tube.
[0010] As a preferred technical scheme of the present application, the electric heating pipe is arranged on the outer arc surface of the inner container, the number of the reaction tubes is several, the air guide pipe is connected to the inside of the air inlet, and one end of the air guide pipe is connected to the outside of the heat preservation box.
[0011] As a preferred technical scheme of the present application, the air guide pipe is connected to the outside of the heat preservation box, and the pump is fixedly arranged on one side of the heat preservation box, and the temperature sensor is fixedly arranged on the upper surface of the heat preservation box.
[0012] As a preferred technical scheme of the present application, the upper surface of the reaction tube is movably sleeved with the cleaning assembly, the cleaning assembly comprises a connecting piece movably sleeved on the top of the reaction tube, and the inner arc surface of the top of the connecting piece is provided with a rubber ring.
[0013] As a preferred technical scheme of the present application, the inner top wall of the connecting piece is movably connected with a bearing near the middle part of the rubber ring, the inner arc surface of the bearing is movably connected with a rotating rod, the outer arc surface of one end of the rotating rod is fixedly provided with a brush, and one end of the brush is connected to the inside of the reaction tube.
[0014] (Three) beneficial effects
[0015] 1. By arranging the electric heating pipe, the temperature of the reaction tube is greatly adjusted, by arranging the pump, air is blown to the cavity between the inner container and the wear-resistant outer tube, and by arranging the heating plate, the temperature of the air is changed, so that the temperature of the inner container is slightly adjusted, and the two are matched with each other, so that the temperature in the reactor is adjusted and controlled, the temperature in the reactor is kept in the best temperature range required by the internal reaction, and the efficiency of the synthesis reaction is improved.
[0016] 2. By the cleaning assembly, the residual reagents and by-products on the inner wall of the reaction tube can be efficiently and completely removed, so that the reaction tube can be restored to a clean state after each round of polypeptide synthesis reaction, and reliable hardware support is provided for subsequent synthesis operation, the good performance of the equipment is effectively maintained, and the service life of the equipment is prolonged. BRIEF DESCRIPTION OF DRAWINGS
[0017] Figure 1 It is a schematic view of the reaction tube structure of a polypeptide synthesis reaction device;
[0018] Figure 2 It is a schematic view of the overall cross-sectional structure of a polypeptide synthesis reaction device;
[0019] Figure 3It is a kind of polypeptide synthesis reaction device's cleaning component structure schematic view;
[0020] Figure 4 It is a kind of polypeptide synthesis reaction device's overall plan structure schematic view;
[0021] Figure 5 It is a kind of polypeptide synthesis reaction device's incubator structure schematic view.
[0022] In the figure: 1, incubator;2, wear-resistant outer tube;201, ladder end;202, exhaust port;203, air inlet;3, heating plate;4, heat-conducting plate;5, reaction tube;501, bending end;6, inner container;601, arc end;7, electric heating tube;8, air duct;9, pump;10, temperature sensor;11, cleaning component;111, connecting piece;112, rubber ring;113, bearing;114, rotating rod;115, brush. DETAILED DESCRIPTION
[0023] The technical scheme in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.
[0024] The present application provides a kind of polypeptide synthesis reaction device, such as Figures 1 to 5As shown, including the incubator 1, the inner top wall of the incubator 1 is fixedly installed with the wear-resistant outer tube 2, the inner arc surface top end of the wear-resistant outer tube 2 is provided with the stepped end 201, the outer arc surface side edge of the wear-resistant outer tube 2 is provided with the air outlet 202, the lower surface middle part of the wear-resistant outer tube 2 is provided with the air inlet 203, the inner arc surface of the wear-resistant outer tube 2 is sleeved with the heating plate 3, the heating plate 3 is attached to the inner arc surface of the wear-resistant outer tube 2, the lower surface of the heating plate 3 is placed with the heat conduction plate 4, the surface of the heat conduction plate 4 is close to the air inlet 203, the air inlet 203 and the air outlet 202 are vertically placed, the top stepped end 201 of the wear-resistant outer tube 2 is movably sleeved with the reaction tube 5, the inner arc surface bottom of the reaction tube 5 is provided with the bent end 501, the upper surface of the bent end 501 is movably sleeved with the inner container 6, the upper surface of the inner container 6 is provided with the arc-shaped end 601, the arc-shaped end 601 is placed on the surface of the bent end 501, one end of the inner container 6 penetrates and connects to the inside of the wear-resistant outer tube 2, the gap between the bent end 501 and the wear-resistant outer tube 2 is provided with the electric heating tube 7, the electric heating tube 7 is placed on the outer arc surface of the inner container 6, the number of the reaction tube 5 is several, the several air inlets 203 are penetrated and connected with the air guide pipe 8, one end of the air guide pipe 8 penetrates to the outside of the incubator 1, the air guide pipe 8 penetrates to the outside of the incubator 1 and is fixedly installed with the pump 9, the pump 9 is placed on one side of the incubator 1, the temperature sensor 10 is fixedly installed on the upper surface side edge of the incubator 1.
[0025] In use, when the reaction tube 5 needs to be heated to a large extent, the inner container 6 is heated by the heating plate 3, the electric heating tube 7 is turned off when the temperature approaches the preset value, the pump 9 is started, air is blown into the cavity between the wear-resistant outer tube 2 and the inner container 6, the air temperature is changed by adjusting the working power of the electric heating tube 7, the temperature in the inner container 6 is adjusted slightly, and finally the temperature in the inner container 6 meets the optimum temperature for the reaction.
[0026] The polypeptide synthesis reactor adjusts the temperature of the reaction tube 5 to a large extent by setting the electric heating tube 7, adjusts the temperature in the inner container 6 to a small extent by setting the pump 9 to blow air into the cavity between the inner container 6 and the wear-resistant outer tube 2 and changing the air temperature by the heating plate 3, and adjusts the temperature in the reactor to a small extent by the two, so as to keep the temperature in the reactor within the optimum temperature range required for the internal reaction and improve the efficiency of the synthesis reaction.
[0027] The upper surface of the reaction tube 5 is movably sleeved with the cleaning assembly 11, the cleaning assembly 11 includes a connecting piece 111 movably sleeved on the top of the reaction tube 5, a rubber ring 112 is arranged on the inner arc surface top of the connecting piece 111, a bearing 113 is movably connected to the inner top wall of the connecting piece 111 close to the middle part of the rubber ring 112, a rotating rod 114 is movably connected to the inner arc surface of the bearing 113, a brush 115 is fixedly installed on the outer arc surface of one end of the rotating rod 114, and one end of the brush 115 penetrates into the reaction tube 5.
[0028] In the design of the device, the brush 115 is arranged between the reaction tube 5 and the inner container 6, and meanwhile, one end of the bearing 113 extends outward, penetrates the connecting piece 111, and is provided with a micro motor, when the micro motor is started, the bearing 113 is driven to rotate, and then the rubber ring 112 connected therewith can be attached to the inner arc surface of the reaction tube 5, the reaction tube 5 is stably arranged in the reasonable interval between the rubber ring 112 and the bearing 113, in this way, with the rotation of the outer ring of the bearing 113, the brush 115 connected therewith also rotates synchronously at high speed, the brush 115 continuously contacts the inner surface of the reaction tube 5, clean water is timely introduced, and the residual reagent and by-product on the inner wall of the reaction tube 5 are efficiently and completely removed by the scouring force of the water flow and the mechanical friction cleaning effect of the brush 115, the two complement each other, the reaction tube 5 can be restored to a clean state after each round of polypeptide synthesis reaction, reliable hardware guarantee is provided for subsequent synthesis operation, the good performance of the equipment is effectively maintained, and the service life of the equipment is prolonged.
[0029] The above merely describes a preferred specific embodiment of the present application, but the protection scope of the present application is not limited to this, any person skilled in the art can make equivalent replacement or change according to the technical scheme and the inventive concept of the present application within the technical range disclosed by the present application, which should be covered in the protection scope of the present application.
Claims
1. A reaction apparatus for polypeptide synthesis comprising an incubator (1), characterised in that: The inner top wall of the incubator (1) is fixedly provided with a wear-resistant outer tube (2), the inner arc top end of the wear-resistant outer tube (2) is provided with a stepped end (201), the outer arc side edge of the wear-resistant outer tube (2) is provided with an air outlet (202), the lower surface of the wear-resistant outer tube (2) is provided with an air inlet (203), the inner arc surface of the wear-resistant outer tube (2) is provided with a heating plate (3), the heating plate (3) is attached to the inner arc surface of the wear-resistant outer tube (2), the lower surface of the heating plate (3) is provided with a heat-conducting plate (4), the surface of the heat-conducting plate (4) is close to the air inlet (203), the air inlet (203) and the air outlet (202) are vertically placed, the top stepped end (201) of the wear-resistant outer tube (2) is movably sleeved with a reaction tube (5), the inner arc bottom of the reaction tube (5) is provided with a bent end (501), the upper surface of the bent end (501) is movably sleeved with an inner container (6), and the upper surface of the inner container (6) is provided with an arc end (601).
2. The polypeptide synthesis reaction apparatus according to claim 1, wherein: The arc end (601) is placed on the surface of the bent end (501), one end of the inner container (6) penetrates and is connected to the inside of the wear-resistant outer tube (2), and the gap between the bent end (501) and the wear-resistant outer tube (2) is provided with an electric heating tube (7).
3. The polypeptide synthesis reaction apparatus according to claim 2, wherein: The electric heating tube (7) is placed on the outer arc surface of the inner container (6), the number of the reaction tube (5) is several, the air inlet (203) is provided with a plurality of air guide pipes (8), one end of the air guide pipe (8) penetrates to the outside of the incubator (1).
4. The polypeptide synthesis reaction apparatus according to claim 3, wherein: The air guide pipe (8) penetrates to the outside of the incubator (1) and is fixedly provided with a pump (9), the pump (9) is placed on one side of the incubator (1), and a temperature sensor (10) is fixedly installed on the upper surface of the incubator (1).
5. The polypeptide synthesis reaction apparatus according to claim 3, wherein: The upper surface of the reaction tube (5) is movably sleeved with a cleaning assembly (11), the cleaning assembly (11) comprises a connecting piece (111) movably sleeved on the top of the reaction tube (5), and the inner arc top of the connecting piece (111) is provided with a rubber ring (112).
6. The polypeptide synthesis reaction apparatus according to claim 5, wherein: The inner top wall of the connecting piece (111) is movably connected with a bearing (113) close to the middle part of the rubber ring (112), the inner arc surface of the bearing (113) is movably connected with a rotating rod (114), the outer arc surface of one end of the rotating rod (114) is fixedly provided with a brush (115), and one end of the brush (115) penetrates into the reaction tube (5).