Nucleic acid pretreatment kit based on gene methylation detection

Abstract

The utility model discloses a nucleic acid pretreatment agent box based on gene methylation detection, which relates to the technical field of medical supplies and comprises a storage component and a shaking component, the storage assembly comprises a box body and a protection assembly, a box cover is hinged to the top end of the box body, a clamping base is arranged on the front face of the box cover, a handle is arranged on the back face of the box cover, a first buffering pad is arranged on the inner wall of the box cover, and a second buffering pad is arranged on the inner wall of one side of the box body. The shake-up assembly comprises a supporting table and a pop-up assembly; the test tube shaking device has the beneficial effects that the double-shaft motor drives the two cams to rotate through the two transmission rods, the two cams are matched with the first tension spring to realize the reciprocating motion of the shaking shell, the shaking shell drives the test tube to move through the lifting block and the test tube shell, and a gene detection sample and a nucleic acid pretreatment agent in the test tube can be automatically shaken up; the operation is more convenient.

Description

TECHNICAL FIELD

[0001] The utility model relates to a nucleic acid pretreatment kit, especially to a nucleic acid pretreatment kit based on gene methylation detection belongs to medical supplies technical field. BACKGROUND

[0002] DNA methylation changes genetic expression without changing DNA sequence, and is often used for early cancer determination, and the detection sample can be obtained from blood, urine, saliva and other tissue samples, the sample to be detected and the pretreatment agent are put into the reagent tube and shaken, then the reagent tube is sealed and loaded into the reagent box, and then transported to the detection equipment of the laboratory of the inspection department for gene methylation detection.

[0003] Among them, the "pulmonary carcinoma gene methylation detection kit" disclosed in the application number "CN202023084587.9" "includes a mounting plate, and the front outer surface of the mounting plate is provided with a reagent box. The pulmonary carcinoma gene methylation detection kit can limit the reagent bottle placed in the reagent box, is more stable than direct placement, reduces the problem of liquid leakage caused by deviation, and can limit and press the reagent bottle. When the methylation DNA extraction chamber and the detection chamber are not in the same place and the reagent box needs to be transported, the reagent bottle in the reagent box may shake and leak. On the one hand, it avoids the accidental loss caused by high extraction cost and liquid leakage, and on the other hand, it avoids cross infection caused by liquid adhesion when the detection personnel opens the reagent box, effectively enhances the safety protection of the detection personnel, thereby improving the sales volume of the product and enhancing the market competitiveness of the manufacturer and enterprise. Through further retrieval, it is found that the "DNA methylation kit" disclosed in the application number "CN202121566582.1" "includes a reagent box shell, a reagent box body, an insertion body and a protective cover. The inside of the reagent box shell is provided with a reagent box body, and the inside of the reagent box body is provided with an insertion body. The upper end of the reagent box shell is provided with a protective cover. The upper and lower ends of the insertion body are provided with movable grooves, and the movable grooves are movably provided with extrusion blocks through anti-disengagement structures. The inside of the movable groove is provided with a stretchable spring between the inside wall of the movable groove and the side of the extrusion block away from the inner wall of the reagent box body. The problem that the insertion body is not convenient for preventing the reagent tube from being collided is solved, the convenience of operation is improved, the mutual collision between the reagent tube and the inner wall of the insertion body during transportation is prevented, damage is caused, and the protection of the reagent tube by the insertion body is improved.

[0004] However, the above two methods still have the following defects: before storing the collected gene detection samples, the gene detection samples and the nucleic acid pretreatment agent need to be shaken manually, which is not convenient. UTILITY MODEL CONTENT

[0005] The utility model discloses a purpose lies in providing a nucleic acid pretreatment kit based on gene methylation detection to solve the problem that the prior art needs to shake up numerous gene detection samples and nucleic acid pretreatment agents manually before storing the collected gene detection samples.

[0006] To realize above-mentioned purpose, the utility model provides the following technical scheme: a nucleic acid pretreatment kit based on gene methylation detection, including receiving subassembly and shake even subassembly, the receiving subassembly includes box body and protection subassembly, the top of box body is hinged with box cover, the front of box cover is equipped with the seat, the back of box cover is equipped with the handle, the inner wall of box cover is equipped with first buffer pad, the inner wall of one side of box body is equipped with second buffer pad, the shake even subassembly includes support platform and pop -on subassembly, the inner wall of bottom of box body is provided with support platform, the top of support platform is equipped with double -shaft motor through motor frame, two transmission shafts of double -shaft motor all are equipped with transmission rod, and the surface of two transmission rods all is equipped with cam, and the surface sliding connection of two cams has shake even shell.

[0007] As a preferred technical scheme of the utility model, the first tension spring is arranged between the shake even shell and the box body, the surface of the shake even shell is slidingly connected with two guide blocks, the front surface of one of the guide blocks is connected with the inner wall of the front surface of the box body, the back surface of the other guide block is connected with the inner wall of the back surface of the box body, one side of the shake even shell is provided with a sliding cylinder, the inner wall of the sliding cylinder is slidingly connected with a sliding rod, one end of the sliding rod is connected with the inner wall of the box body, the sliding rod and the sliding cylinder are arranged in the first tension spring, the inner walls of the two sides of the shake even shell are both provided with a stop block, and the third buffer pad is arranged between the two stop blocks and the shake even shell.

[0008] As a preferred technical scheme of the utility model, the pop -on subassembly includes a connecting block, the connecting block is arranged on the inner wall of the back surface of the shake even shell, the surface of the connecting block is slidingly connected with a lifting block, the surface of the connecting block is provided with a sliding hole, the inner wall of the sliding hole is slidingly connected with a pressing rod, one end of the pressing rod is provided with a moving frame, the second tension spring is arranged between the moving frame and the connecting block, the second tension spring is arranged on the outer side of the pressing rod, and the inner wall of the moving frame is slidingly connected with an E-shaped plate.

[0009] As a preferred technical scheme of the utility model, the E-shaped plate and the lifting block are hinged with four first transmission plates through a hinge seat, and the E-shaped plate and the box body are hinged with four second transmission plates through a hinge seat.

[0010] As a preferred technical scheme of the utility model, the surface of the lifting block is slidably connected with the inner wall of the shaking shell, the surface of the lifting block is respectively provided with a first through hole and a second through hole, the inner wall of the first through hole is provided with a test tube shell, the inner wall of the second through hole is provided with a reagent bottle shell, the inner wall of the bottom end of the reagent bottle shell and the inner wall of the bottom end of the test tube shell are both provided with rubber pads, and the contact parts of the lifting block and the two third buffer pads are both slidably connected.

[0011] As a preferred technical scheme of the utility model, the inner wall of the box body is provided with a storage battery, the contact parts of the box body and the two transmission rods are both rotatably connected through bearings, the other side of the box body is provided with a charging port electrically connected with the storage battery, the other side of the box body is provided with two T-shaped grooves, the front surface of the box body is provided with a buckle clamped with the clamping seat, and the top end of the box body is provided with a switch panel.

[0012] As a preferred technical scheme of the utility model, the protection assembly comprises a protection shell, the protection shell is slidably arranged on the other side of the box body, one side of the protection shell is provided with two T-shaped blocks slidably connected with the T-shaped grooves, the inner wall of the protection shell is provided with two iron sheets, the front surface of the protection shell is slidably connected with a protection plate, the inner wall of the protection plate is provided with a magnet sheet adsorbed with the iron sheet, one side of the protection plate is connected with the other side of the box body, the protection shell is sleeved on the outside of the charging port, and the top end and the bottom end of the protection shell are both provided with anti-skid lines.

[0013] Compared with the related art, the nucleic acid pretreatment kit based on gene methylation detection has the following beneficial effects:

[0014] 1. The double-shaft electric motor drives the two cams to rotate through the two transmission rods, the reciprocating motion of the shaking shell can be realized through the cooperation of the two cams and the first tension spring, the test tube shell drives the test tube to move through the lifting block, the gene detection sample and the nucleic acid pretreatment agent in the test tube can be automatically shaken, and the utility model is more convenient.

[0015] 2. When the box cover is opened, the compression rod loses the limitation of the box cover, the second tension spring is shortened and drives the moving frame to move upwards, the test tube shell and the reagent bottle shell are driven to move upwards through the E-shaped plate, the first transmission plate and the lifting block, the height of the test tube and the reagent bottle is lifted, the height of the top end of the test tube and the height of the top end of the reagent bottle are both higher than the height of the top end plane of the box body, and the test tube and the reagent bottle are convenient to take off.

[0016] 3. The protection shell is limited by the T-shaped groove through the T-shaped block, the protection shell is fixedly connected with the protection plate through the iron sheet and the magnet sheet, the protection shell separates the charging port from the outside, and the protection of the charging port is realized. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is a structural schematic view of the utility model.

[0018] Figure 2 is a cross-sectional structure schematic view of the utility model;

[0019] Figure 3 is a cross-sectional structure schematic view of the utility model from one side;

[0020] Figure 4 is a cross-sectional structure schematic view of the utility model from the other side;

[0021] Figure 5 is a back structure schematic view of the utility model;

[0022] Figure 6 is a structure schematic view of the E-shaped plate of the utility model;

[0023] Figure 7 is a structure schematic view of the protective assembly of the utility model;

[0024] Figure 8 is a top view cross-sectional structure schematic view of the protective assembly of the utility model.

[0025] In the figure: 1, storage assembly; 101, box body; 102, box cover; 103, card seat; 104, first buffer pad; 105, second buffer pad; 106, storage battery; 107, buckle; 108, switch panel; 2, shake evenly assembly; 201, support table; 202, double-shaft motor; 203, transmission rod; 204, cam; 205, shake evenly shell; 206, first tension spring; 207, guide block; 208, sliding cylinder; 209, sliding rod; 210, stop block; 211, third buffer pad; 3, pop-out assembly; 301, connecting block; 302, lifting block; 303, sliding hole; 304, pressing rod; 305, moving frame; 306, second tension spring; 307, E-shaped plate; 308, first transmission plate; 309, second transmission plate; 310, first through hole; 311, second through hole; 312, test tube shell; 313, reagent bottle shell; 4, protective assembly; 401, protective shell; 402, T-shaped block; 403, protective plate. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0027] Please refer to Figures 1-8The utility model provides a nucleic acid pretreatment kit based on gene methylation detection, including receiving subassembly 1 and shake even subassembly 2,

[0028] Receiving subassembly 1 includes box body 101 and protection assembly 4, the top of box body 101 is hinged with box cover 102, the front of box cover 102 is fixedly provided with clamping seat 103, clamping seat 103 is provided with two, the back of box cover 102 is fixedly provided with handle, the inner wall of box cover 102 is fixedly provided with first buffer pad 104, the inner wall of one side of box body 101 is fixedly provided with second buffer pad 105,

[0029] The inner wall of box body 101 is fixedly provided with battery 106, the contact part of box body 101 with two transmission rods 203 is all connected through bearing rotation, the other side of box body 101 is fixedly provided with the charging port of electric connection with battery 106, the other side of box body 101 is provided with two T-shaped grooves, the front of box body 101 is fixedly provided with the buckle 107 of clamping with clamping seat 103, buckle 107 is provided with two, the top of box body 101 is fixedly provided with switch panel 108, the clamping of buckle 107 with clamping seat 103 realizes the fixing of box cover 102 with box body 101, the surface of switch panel 108 is equipped with double-shaft motor switch, double-shaft motor 202 will be electrically connected with battery 106 through double-shaft motor switch,

[0030] Specifically, when box cover 102 is attached with box body 101, clamping seat 103 can be clamped with buckle 107, the fixing of box cover 102 with box body 101 is realized, first buffer pad 104 will separate the test tube arranged inside test tube shell 312 with box cover 102, and the test tube is protected, and the first buffer pad 104 will protect the pretreatment reagent bottle arranged inside reagent bottle shell 313, and second buffer pad 105 separates the inner wall of one side of box body 101 from shake even shell 205, to avoid that shake even shell 205 directly collides with box body 101, and it is safer.

[0031] Shake even subassembly 2 includes support table 201 and pop-up assembly 3, support table 201 is fixedly arranged on the inner wall at the bottom of box body 101, double-shaft motor 202 is fixedly arranged on the top of support table 201 through motor bracket, the two transmission shafts of double-shaft motor 202 are fixedly provided with transmission rod 203, the surfaces of two transmission rods 203 are fixedly provided with cam 204, two cams 204 are slidably connected with shake even shell 205, start double-shaft motor 202, double-shaft motor 202 will drive two transmission rods 203 to rotate, two transmission rods 203 will drive two cams 204 to rotate, cam 204 will push and shove shake even shell 205, and the transverse movement of shake even shell 205 is realized,

[0032] The first tension spring 206 is fixed between the shell 205 and the box body 101, the shell 205 is provided with two first tension springs 206, the surface of the shell 205 is slidably connected with two guide blocks 207, one of the guide blocks 207 is fixedly connected with the inner wall of the front surface of the box body 101, the back surface of the other guide block 207 is fixedly connected with the inner wall of the back surface of the box body 101, one side of the shell 205 is fixedly provided with a sliding cylinder 208, the sliding cylinder 208 is provided with two, the inner wall of the sliding cylinder 208 is slidably connected with a sliding rod 209, one end of the sliding rod 209 is fixedly connected with the inner wall of the box body 101, the sliding rod 209 and the sliding cylinder 208 are arranged in the first tension spring 206, the inner wall of both sides of the shell 205 is fixedly provided with a stop block 210, the two stop blocks 210 and the shell 205 are fixedly provided with a third buffer pad 211, the first tension spring 206 is elongated when the shell 205 is pushed, the first tension spring 206 is shortened and exerts a pulling force on the shell 205 when the convex part of the cam 204 is away from the shell 205, so as to make the shell 205 fit the cam 204, realize the reciprocating motion of the shell 205, the sliding cylinder 208 and the sliding rod 209 limit the deformation degree of the first tension spring 206, avoid the bending of the first tension spring 206 when it is contracted, prolong its service life, the guide block 207 guides and limits the vertical direction of the shell 205, improves the stability of the shell 205 when moving;

[0033] Specifically, first start the double-shaft motor 202, the double-shaft motor 202 drives the two transmission rods 203 to rotate, the two transmission rods 203 drive the two cams 204 to rotate, the two cams 204 push the shell 205, realize the horizontal movement of the shell 205, the first tension spring 206 is elongated when the shell 205 is pushed, the first tension spring 206 is shortened and exerts a pulling force on the shell 205 when the convex part of the cam 204 is away from the shell 205, so as to make the shell 205 fit the cam 204, realize the reciprocating motion of the shell 205, the shell 205 drives the test tube to move through the lifting block 302 and the test tube shell 312, can automatically shake the gene detection sample and nucleic acid pretreatment agent in the test tube, more convenient.

[0034] The ejection assembly 3 includes a connecting block 301 fixedly arranged on the inner wall of the back surface of the shell 205, the surface of the connecting block 301 is slidably connected with a lifting block 302, the surface of the connecting block 301 is provided with a sliding hole 303, the inner wall of the sliding hole 303 is slidably connected with a pressing rod 304, one end of the pressing rod 304 is fixedly provided with a moving frame 305, the second tension spring 306 is fixedly arranged between the moving frame 305 and the connecting block 301, the second tension spring 306 is arranged outside the pressing rod 304, the inner wall of the moving frame 305 is slidably connected with an E-shaped plate 307, the pressing rod 304 is pressed when the box cover 102 rotates downward, the pressing rod 304 pushes the moving frame 305 to move downward, the moving frame 305 moves downward and exerts pressure on the E-shaped plate 307;

[0035] Four first transmission plates 308 are hingedly connected between the E-shaped plate 307 and the lifting block 302 through the hinge seat, and four second transmission plates 309 are hingedly connected between the E-shaped plate 307 and the box body 101 through the hinge seat. When the E-shaped plate 307 is pressed by the moving frame 305, the E-shaped plate 307 will move obliquely downward due to the limitation of the first transmission plate 308, the second transmission plate 309 and the hinge seat. The E-shaped plate 307 slides relative to the moving frame 305. The E-shaped plate 307 pulls down the lifting block 302 through the first transmission plate 308, lowers the height of the lifting block 302, the test tube shell 312 and the reagent bottle shell 313, and promotes the test tube and the reagent bottle to retract into the inside of the uniform shaking shell 205.

[0036] The surface of the lifting block 302 is slidingly connected with the inner wall of the uniform shaking shell 205. The surface of the lifting block 302 is respectively provided with a first through hole 310 and a second through hole 311. The first through hole 310 is provided with twelve first through holes 310, and the second through hole 311 is provided with three second through holes 311. The inner wall of the first through hole 310 is fixedly provided with the test tube shell 312, and the inner wall of the second through hole 311 is fixedly provided with the reagent bottle shell 313. The inner wall of the bottom end of the reagent bottle shell 313 and the inner wall of the bottom end of the test tube shell 312 are both fixedly provided with rubber pads. The contact part of the lifting block 302 with the two third buffer pads 211 is slidingly connected. The reagent bottle shell 313 is used for accommodating the test tube or the empty test tube which has collected the genetic detection sample. The reagent bottle shell 313 accommodates the pretreatment reagent bottle. The rubber pads protect the bottom of the test tube and the bottom end of the reagent bottle.

[0037] Specifically, when the box cover 102 is opened, the pressing rod 304 will lose the limit of the box cover 102. At this time, the second tension spring 306 will be shortened and drive the moving frame 305 to move upward. The moving frame 305 will slide along the surface of the E-shaped plate 307 and drive the E-shaped plate 307 to move upward. The E-shaped plate 307 drives the lifting block 302 to move upward through the first transmission plate 308. The lifting block 302 drives the test tube shell 312 and the reagent bottle shell 313 to move upward, which raises the height of the test tube and the reagent bottle. The height of the top end of the test tube and the height of the top end of the reagent bottle are both higher than the height of the top end plane of the box body 101, which facilitates the removal of the test tube and the reagent bottle. The lifting block 302 is stopped by the third buffer pad 211, and the stop block 210 limits the lifting block 302 through the third buffer pad 211 to prevent it from separating from the uniform shaking shell 205.

[0038] The protection assembly 4 comprises a protection shell 401 which is slidingly arranged on the other side of the box body 101, two T-shaped blocks 402 which are slidingly arranged in the T-shaped slots are arranged on one side of the protection shell 401, two iron sheets are arranged on the inner wall of the protection shell 401, a protection plate 403 is slidingly connected to the front of the protection shell 401, a magnet sheet is arranged on the inner wall of the protection plate 403 and is attracted to the iron sheet, one side of the protection plate 403 is fixedly connected to the other side of the box body 101, the protection shell 401 is arranged outside the charging port, and anti-skid lines are arranged on the top end and the bottom end of the protection shell 401.

[0039] Specifically, the protection shell 401 is limited by the T-shaped blocks 402 in the T-shaped slots, the protection shell 401 is fixedly connected to the protection plate 403 through the iron sheets and the magnet sheet, the protection shell 401 separates the charging port from the outside, thereby protecting the charging port, when the charging port needs to be used, the protection shell 401 is clamped by the anti-skid lines, then the protection shell 401 is pulled longitudinally, the T-shaped blocks 402 are driven to slide along the T-shaped slots longitudinally, the protection shell 401 is separated from the protection plate 403, and the charging port is exposed, which is very convenient.

[0040] In use, first, the gene detection samples are collected, and an appropriate amount of methylation pretreatment agent is added into the inside of the test tube in which the gene detection samples have been collected, the test tube is slid into the test tube shell 312, the test tube is arranged, and the operation is repeated until the quantity requirement of the gene detection samples is met, then the box cover 102 is rotated downward, the box cover 102 extrudes the pressing rod 304 when being rotated downward, the pressing rod 304 pushes the moving frame 305 to move downward, the moving frame 305 moves downward and exerts pressure on the E-shaped plate 307, the E-shaped plate 307 moves obliquely downward due to the limitation of the first transmission plate 308, the second transmission plate 309 and the hinge seat, the E-shaped plate 307 relatively slides with the moving frame 305, the E-shaped plate 307 pulls the lifting block 302 downward through the first transmission plate 308, the height of the lifting block 302, the test tube shell 312 and the reagent bottle shell 313 is lowered, the test tube and the reagent bottle are driven to retract into the inside of the uniform shaking shell 205, the height of the top end of the test tube and the height of the top end of the reagent bottle are lower than the height of the top end of the uniform shaking shell 205, and the first buckle 107 is buckled with the first clamping seat 103;

[0041] Then start the double-shaft motor 202, the double-shaft motor 202 will drive two transmission rods 203 to rotate, two transmission rods 203 will drive two cams 204 to rotate, two cams 204 will push the shaking shell 205, realize the lateral movement of the shaking shell 205, the first tension spring 206 is elongated when the shaking shell 205 is pushed, when the convex part of the cam 204 is away from the shaking shell 205, the first tension spring 206 shortens and exerts a pulling force on the shaking shell 205, prompting the shaking shell 205 to fit the cam 204, realizing the reciprocating motion of the shaking shell 205, the shaking shell 205 drives the test tube to move through the lifting block 302 and the test tube shell 312, and can automatically shake the gene detection sample and nucleic acid pretreatment agent in the test tube, which is more convenient.

[0042] It should be noted that when the box cover 102 is opened, the pressure rod 304 loses the limit of the box cover 102, at this time the second tension spring 306 will shorten and drive the moving frame 305 to move upwards, the moving frame 305 drives the lifting block 302 to move upwards through the E-shaped plate 307 and the first transmission plate 308, the lifting block 302 drives the test tube shell 312 and the reagent bottle shell 313 to move upwards, which raises the height of the test tube and the reagent bottle, so that the height of the top end of the test tube and the height of the top end of the reagent bottle are both higher than the height of the top end plane of the box body 101, facilitating the removal of the test tube and the reagent bottle, the lifting block 302 is stopped by the third buffer pad 211, the stop block 210 limits the lifting block 302 through the third buffer pad 211, preventing it from being separated from the shaking shell 205, the protective shell 401 is limited by the T-shaped groove through the T-shaped block 402, the protective shell 401 is fixedly connected with the protective plate 403 through the iron sheet and the magnet sheet, and the protective shell 401 separates the charging port from the outside, realizing the protection of the charging port.

[0043] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and modifications can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid pre-treatment kit based on detection of genetic methylation, characterized by, Comprising The storage assembly (1) comprises a box body (101) and a protection assembly (4), the top end of the box body (101) is hinged with a box cover (102), the front surface of the box cover (102) is provided with a card seat (103), the back surface of the box cover (102) is provided with a handle, the inner wall of the box cover (102) is provided with a first buffer pad (104), and the inner wall of one side of the box body (101) is provided with a second buffer pad (105); The uniform shaking assembly (2) comprises a support table (201) and a pop-up assembly (3), the support table (201) is arranged on the inner wall of the bottom end of the box body (101), the top end of the support table (201) is provided with a double-shaft motor (202) through a motor bracket, the two transmission shafts of the double-shaft motor (202) are provided with transmission rods (203), the surfaces of the two transmission rods (203) are provided with cams (204), and the surfaces of the two cams (204) are slidably connected with a uniform shaking shell (205).

2. The nucleic acid pre-treatment kit based on gene methylation detection according to claim 1, characterized in that: First tension springs (206) are arranged between the uniform shaking shell (205) and the box body (101), the surfaces of the two guide blocks (207) are slidably connected with the uniform shaking shell (205), one of the guide blocks (207) is connected with the inner wall of the front surface of the box body (101), the other guide block (207) is connected with the inner wall of the back surface of the box body (101), one side of the uniform shaking shell (205) is provided with a sliding cylinder (208), the inner wall of the sliding cylinder (208) is slidably connected with a sliding rod (209), one end of the sliding rod (209) is connected with the inner wall of the box body (101), and the sliding rod (209) and the sliding cylinder (208) are arranged in the first tension spring (206). The inner walls of the two sides of the uniform shaking shell (205) are provided with stop blocks (210), and the third buffer pads (211) are arranged between the two stop blocks (210) and the uniform shaking shell (205).

3. The nucleic acid pre-treatment kit based on gene methylation detection according to claim 2, characterized in that: The pop-up assembly (3) comprises a connecting block (301), the connecting block (301) is arranged on the inner wall of the back surface of the uniform shaking shell (205), the surface of the connecting block (301) is slidably connected with a lifting block (302), the surface of the connecting block (301) is provided with a sliding hole (303), the inner wall of the sliding hole (303) is slidably connected with a pressing rod (304), one end of the pressing rod (304) is provided with a moving frame (305), the second tension spring (306) is arranged between the moving frame (305) and the connecting block (301), the second tension spring (306) is arranged on the outside of the pressing rod (304), and the inner wall of the moving frame (305) is slidably connected with an E-shaped plate (307).

4. The nucleic acid pre-treatment kit based on gene methylation detection according to claim 3, characterized in that: Four first transmission plates (308) are hinged between the E-shaped plate (307) and the lifting block (302) through a hinge seat, and four second transmission plates (309) are hinged between the E-shaped plate (307) and the box body (101) through a hinge seat.

5. The nucleic acid pre-treatment kit based on gene methylation detection according to claim 3, characterized in that: The surface of the lifting block (302) is in sliding connection with the inner wall of the mixing shell (205), the surface of the lifting block (302) is respectively provided with a first through hole (310) and a second through hole (311), the inner wall of the first through hole (310) is provided with a test tube shell (312), the inner wall of the second through hole (311) is provided with a reagent bottle shell (313), the inner wall of the bottom end of the reagent bottle shell (313) and the inner wall of the bottom end of the test tube shell (312) are both provided with rubber pads, and the contact parts of the lifting block (302) and the two third buffer pads (211) are both in sliding connection.

6. The nucleic acid pre-treatment kit based on gene methylation detection according to claim 1, characterized in that: The inner wall of the box body (101) is provided with a battery (106), the contact parts of the box body (101) and the two transmission rods (203) are both rotationally connected through bearings, the other side of the box body (101) is provided with a charging port in electrical connection with the battery (106), the other side of the box body (101) is provided with two T-shaped grooves, the front side of the box body (101) is provided with a buckle (107) in clamping connection with the clamping seat (103), and the top end of the box body (101) is provided with a switch panel (108).

7. The nucleic acid pre-treatment kit based on gene methylation detection according to claim 6, characterized in that: The protection assembly (4) comprises a protection shell (401), the protection shell (401) is slidably arranged on the other side of the box body (101), one side of the protection shell (401) is provided with two T-shaped blocks (402) in sliding connection with the T-shaped grooves, the inner wall of the protection shell (401) is provided with two iron sheets, the front side of the protection shell (401) is slidably connected with a protection plate (403), the inner wall of the protection plate (403) is provided with a magnet sheet in adsorption connection with the iron sheet, one side of the protection plate (403) is connected with the other side of the box body (101), the protection shell (401) is sleeved on the outside of the charging port, and the top end and the bottom end of the protection shell (401) are both provided with anti-skid lines.

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