Protease purification production device

Abstract

The utility model provides a protease purification production device, which belongs to the field of production devices, and comprises a separation tank, a liquid discharge pipe, a sealing cover, a stirring separation mechanism, a material scraping mechanism, a sedimentation tank and a material conveying mechanism, in the scheme, a rotating shaft rotates to drive a filter cylinder A and a filter cylinder B to rotate, and a support disc is arranged to support a mounting cylinder; the mounting cylinder is movably arranged on the rotating shaft in a sleeving manner, so that the mounting cylinder is convenient to disassemble and separate, the mounting cylinder is in threaded connection to the rotating shaft through the locking screw, the mounting cylinder can be fixed to the rotating shaft, the rotating shaft rotates to drive the mounting cylinder to rotate, and the mounting cylinder rotates to drive the filter cylinder A and the filter cylinder B to rotate at the same time; the protease can be primarily separated and purified through the filter drum B, and can be secondarily separated and purified through the filter drum A, so that the problem that filtered impurities cannot be easily removed by a protease purification production device in the prior art can be solved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of production device, more particularly to a protease purification production device. BACKGROUND

[0002] In the production of protease needs to be purified and separated, briefly speaking is that protease in the extraction process first to break the biological tissue, and then take the supernatant and add reagent, then the liquid is filled into enzyme extraction equipment, filter out impurities through the equipment, get high protease content liquid.But the present stage protease purification extraction equipment to the filtration efficiency of solution is low, the problem of impurity removal effect is poor, needs improvement.

[0003] The authorized publication number "CN215916704U" records "a protease purification device, including the sedimentation cylinder, the filter box and the guide pipe between the sedimentation cylinder and the filter box;The sedimentation cylinder is tapered and a thread line is formed on the inner circumferential wall, the upper part of the sedimentation cylinder is provided with a feed pipe, and the lower part of the sedimentation cylinder is communicated with the sediment tank, and the lower part of the sediment tank is provided with a blowdown valve;The upper part of the filter box is rotatably provided with a transmission sleeve, the upper end of the transmission sleeve is located above the filter box and is connected with the upper driving device for driving the rotation, the lower end of the transmission sleeve extends into the interior of the filter box and is fixedly connected with the first upper connecting plate, and the middle of the first upper connecting plate is provided with a through hole with the same inner diameter as the transmission sleeve;The utility model can automatically realize the centrifugal filtration and twice rotary screen filtration of the protease purification solution, effectively improve the filtration efficiency in the protease purification production process, and ensure the impurity removal precision".

[0004] The above-mentioned patent improves the filtration efficiency in the protease purification production process, but the above-mentioned patent is not easy to remove the filtered impurities, because of long time filtration, the filtration effect is reduced, thereby affecting the efficiency of protease purification. Utility model content

[0005] The utility model aims at providing a protease purification production device, which aims at solving the problem that the protease purification production device in the prior art is not easy to remove the filtered impurities.

[0006] To achieve the above object, the utility model provides the following technical scheme:

[0007] A protease purification production device, comprising:

[0008] A separation tank;

[0009] A liquid discharge pipe fixedly connected to the bottom of the separation tank;

[0010] A sealing cover detachably connected to the top of the separation tank;

[0011] A stirring and separating mechanism is arranged on the separation tank, and is used for solid-liquid separation of the protease;

[0012] A scraping mechanism is arranged on the sealing cover;

[0013] A precipitation tank is arranged on one side of the separation tank;

[0014] A material conveying mechanism is arranged on the separation tank and the precipitation tank.

[0015] As a preferred scheme of the present application, the stirring and separating mechanism comprises:

[0016] A driving component is arranged on the separation tank;

[0017] A separating component is arranged on the driving component.

[0018] As a preferred scheme of the present application, the driving component comprises a driving motor, a U-shaped plate, a rotating shaft and a supporting disc, the rotating shaft is rotatably connected to the lower inner wall of the separation tank, one end of the rotating shaft rotatably penetrates the separation tank and extends to the outside of the separation tank, the supporting disc is fixedly connected to the circumferential surface of the rotating shaft, the U-shaped plate is fixedly connected to the bottom of the separation tank, the driving motor is fixedly connected to the bottom of the U-shaped plate, and the output end of the driving motor rotatably penetrates the U-shaped plate and is fixedly connected with the rotating shaft.

[0019] As a preferred scheme of the present application, the separating component comprises a filter cylinder A, a filter cylinder B, a mounting cylinder and a locking screw, the mounting cylinder is movably sleeved on the rotating shaft, the locking screw is movably inserted on the mounting cylinder, one end of the locking screw is threadedly connected to the rotating shaft, the filter cylinder A is fixedly connected to the circumferential surface of the mounting cylinder and rotatably arranged in the separation tank, and the filter cylinder B is fixedly connected to the circumferential surface of the mounting cylinder and located on the inside of the filter cylinder A.

[0020] As a preferred scheme of the present application, the scraping mechanism comprises a scraper A and a scraper B, the scraper A is fixedly connected to the bottom of the sealing cover and abuts against the inner wall of the filter cylinder A, and the scraper B is fixedly connected to the bottom of the sealing cover and abuts against the filter cylinder B.

[0021] As a preferred scheme of the present application, the material conveying mechanism comprises a material conveying pump, a liquid inlet pipe and a liquid outlet pipe, the material conveying pump is arranged between the separation tank and the precipitation tank, the liquid inlet pipe is fixedly connected between the material conveying pump and the precipitation tank, and the liquid outlet pipe is fixedly connected between the material conveying pump and the sealing cover.

[0022] As a preferred scheme of the present application, the sealing cover is detachably connected to the top of the separation tank through bolts.

[0023] As a preferred scheme of the utility model, the bottom of the separation tank is fixedly connected with three supporting legs.

[0024] Compared with the prior art, the utility model has the beneficial effects that:

[0025] 1、In the scheme, the output end rotation of the driving motor can drive the rotation of the rotating shaft, the rotation of the rotating shaft can drive the rotation of the filter cylinder A and the filter cylinder B, the supporting disc is arranged for supporting the mounting cylinder, the mounting cylinder is movably sleeved on the rotating shaft, so that the mounting cylinder is convenient to disassemble and separate, the locking screw is threadedly connected on the rotating shaft, so that the mounting cylinder can be fixed on the rotating shaft, the rotation of the rotating shaft can drive the rotation of the mounting cylinder, the rotation of the mounting cylinder can simultaneously drive the rotation of the filter cylinder A and the filter cylinder B, the filter cylinder B is arranged to preliminarily separate and purify the protease, the filter cylinder A is arranged to twice separate and purify the protease, and the purity of the protease can be improved through twice separation.

[0026] 2、In the scheme, the scraper A is arranged to scrape the inner wall of the filter cylinder A, the scraper B is arranged to scrape the inner side of the filter cylinder B, so as to prevent the inner wall of the filter cylinder A and the scraper B from being blocked and affecting the purification of the protease, and the setting of the feed pump, the liquid inlet pipe and the liquid outlet pipe can convey the protease after precipitation in the precipitation tank into the separation tank for separation and purification.

[0027] 3、In the scheme, the sealing cover is detachably connected on the top of the separation tank through bolts, so that the filter cylinder A and the filter cylinder B are convenient to take out from the separation tank, and the impurities in the filter cylinder A and the filter cylinder B are convenient to remove. BRIEF DESCRIPTION OF DRAWINGS

[0028] The accompanying drawings are included to provide a further understanding of the utility model, and constitute a part of the specification, and are used together with the embodiments of the utility model to explain the utility model, and do not constitute the limitation to the utility model. In the drawings:

[0029] Figure 1 It is the three-dimensional structure schematic view of the utility model;

[0030] Figure 2 It is the sectional view of the utility model;

[0031] Figure 3 It is the utility model Figure 2 It is the enlarged view of A in the utility model;

[0032] Figure 4 It is the explosion view of the filter cylinder A in the utility model.

[0033] In the figure: 1, separation tank; 2, support foot; 3, drive motor; 4, U-shaped plate; 5, feed pump; 6, sedimentation tank; 7, liquid inlet pipe; 8, liquid outlet pipe; 9, sealing cover; 10, liquid discharge pipe; 11, rotating shaft; 12, support disc; 13, filter cartridge A; 14, scraper A; 15, filter cartridge B; 16, scraper B; 17, mounting cylinder; 18, locking screw. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0035] Embodiment 1

[0036] Please refer to Figures 1-4 The technical solutions provided by the embodiment are as follows:

[0037] A proteinase purification production device, which is composed of a separation tank 1, a liquid discharge pipe 10, a sealing cover 9, a stirring and separating mechanism, a scraping mechanism, a sedimentation tank 6 and a feed mechanism, the liquid discharge pipe 10 is fixedly connected to the bottom of the separation tank 1, the sealing cover 9 is detachably connected to the top of the separation tank 1, and the sedimentation tank 6 is arranged on one side of the separation tank 1.

[0038] In the specific embodiment of the utility model, the liquid discharge pipe 10 is arranged to discharge the separated proteinase, the sealing cover 9 is arranged to cover the top of the separation tank 1, and the sedimentation tank 6 is arranged to precipitate the proteinase.

[0039] Specifically, a driving component is arranged on the separation tank 1, the driving component comprises a drive motor 3, a U-shaped plate 4, a rotating shaft 11 and a support disc 12, the rotating shaft 11 is rotatably connected to the lower inner wall of the separation tank 1, one end of the rotating shaft 11 rotatably penetrates the separation tank 1 and extends to the outside of the separation tank 1, the support disc 12 is fixedly connected to the circumferential surface of the rotating shaft 11, the U-shaped plate 4 is fixedly connected to the bottom of the separation tank 1, the drive motor 3 is fixedly connected to the bottom of the U-shaped plate 4, and the output end of the drive motor 3 rotatably penetrates the U-shaped plate 4 and is fixedly connected with the rotating shaft 11.

[0040] In the specific embodiment of the utility model, the output end of the drive motor 3 can drive the rotating shaft 11 to rotate, the rotating shaft 11 can drive the filter cartridge A 13 and the filter cartridge B 15 to rotate, and the support disc 12 is arranged to support the mounting cylinder 17.

[0041] Specifically, the separating component is arranged on the driving component, the separating component comprises filter cylinder A 13, filter cylinder B 15, mounting cylinder 17 and locking screw 18, the mounting cylinder 17 is movably sleeved on the rotating shaft 11, the locking screw 18 is movably inserted on the mounting cylinder 17, one end of the locking screw 18 is threadedly connected on the rotating shaft 11, the filter cylinder A 13 is fixedly connected on the circumferential surface of the mounting cylinder 17, and the filter cylinder A 13 is rotatably arranged in the separating tank 1, and the filter cylinder B 15 is fixedly connected on the circumferential surface of the mounting cylinder 17 and located on the inner side of the filter cylinder A 13.

[0042] In the embodiment of the utility model, the mounting cylinder 17 is movably sleeved on the rotating shaft 11, so that the mounting cylinder 17 is convenient to disassemble and separate, the locking screw 18 is threadedly connected on the rotating shaft 11, so that the mounting cylinder 17 can be fixed on the rotating shaft 11, the rotating shaft 11 can drive the mounting cylinder 17 to rotate, the mounting cylinder 17 can drive the filter cylinder A 13 and the filter cylinder B 15 to rotate simultaneously, the filter cylinder B 15 can be arranged to preliminarily separate and purify protease, the filter cylinder A 13 can be arranged to twice separate and purify protease, and the purity of protease can be improved through twice separation.

[0043] Specifically, the scraping mechanism is arranged on the sealing cover 9, and the scraping mechanism comprises scraper A 14 and scraper B 16; the scraper A 14 is fixedly connected to the bottom of the sealing cover 9 and is attached to the inner wall of the filter cylinder A 13; and the scraper B 16 is fixedly connected to the bottom of the sealing cover 9 and is attached to the filter cylinder B 15.

[0044] In the embodiment of the utility model, the scraper A 14 is arranged to scrape the inner wall of the filter cylinder A 13, the scraper B 16 is arranged to scrape the inner side of the filter cylinder B 15, and the inner walls of the filter cylinder A 13 and the filter cylinder B 16 are prevented from being blocked, so as to affect the purification of protease.

[0045] Specifically, the material conveying mechanism is arranged on the separating tank 1 and the sedimentation tank 6, and the material conveying mechanism comprises a material conveying pump 5, a liquid inlet pipe 7 and a liquid outlet pipe 8; the material conveying pump 5 is arranged between the separating tank 1 and the sedimentation tank 6; the liquid inlet pipe 7 is fixedly connected between the material conveying pump 5 and the sedimentation tank 6; and the liquid outlet pipe 8 is fixedly connected between the material conveying pump 5 and the sealing cover 9.

[0046] In the embodiment of the utility model, the material conveying pump 5, the liquid inlet pipe 7 and the liquid outlet pipe 8 are arranged, so that the protease in the sedimentation tank 6 after sedimentation can be conveyed into the separating tank 1 for separation and purification.

[0047] Specifically, the sealing cover 9 is detachably connected to the top of the separating tank 1 by bolts.

[0048] In the embodiment of the utility model, the sealing cover 9 is detachably connected to the top of the separation tank 1 through bolts, so that the filter cartridge A 13 and the filter cartridge B 15 are convenient to take out from the separation tank 1, and impurities inside the filter cartridge A 13 and the filter cartridge B 15 are convenient to remove.

[0049] Specifically, the bottom of the separation tank 1 is fixedly connected with three supporting legs 2.

[0050] In the embodiment of the utility model, the supporting legs 2 are arranged to support the separation tank 1.

[0051] The working principle or working process of the proteinase purification production device is as follows: the output end of the driving motor 3 is rotated to drive the rotating shaft 11 to rotate, the rotating shaft 11 is rotated to drive the filter cartridge A 13 and the filter cartridge B 15 to rotate, the supporting disc 12 is arranged to support the mounting cylinder 17, the mounting cylinder 17 is movably sleeved on the rotating shaft 11, so that the mounting cylinder 17 is convenient to disassemble and separate, the locking screw 18 is threadedly connected to the rotating shaft 11, so that the mounting cylinder 17 is fixed on the rotating shaft 11, the rotating shaft 11 is rotated to drive the mounting cylinder 17 to rotate, the mounting cylinder 17 is rotated to simultaneously drive the filter cartridge A 13 and the filter cartridge B 15 to rotate, the filter cartridge B 15 is arranged to preliminarily separate and purify the proteinase, the filter cartridge A 13 is arranged to secondarily separate and purify the proteinase, the scraper A 14 is arranged to scrape the inner wall of the filter cartridge A 13, and the scraper B 16 is arranged to scrape the inner side of the filter cartridge B 15.

[0052] Finally, it should be pointed out that: the above is only the preferred embodiment of the utility model, and is not used to limit the utility model, although the utility model has been described in detail with reference to the foregoing embodiments, for those skilled in the art, still can modify the technical scheme recorded in the foregoing embodiments, or make equivalent replacement to part of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model should be included in the protection scope of the utility model.

Claims

1. A device for the purification and production of a protease, characterized in that, The utility model provides a kind of proteinase separation and purification device, including: Separation tank (1); Liquid discharge pipe (10) is fixedly connected to the bottom of separation tank (1); Sealing cover (9) is detachably connected to the top of separation tank (1); Stirring separation mechanism is arranged on separation tank (1), and the stirring separation mechanism is used for proteinase solid-liquid separation; Scraping mechanism is arranged on sealing cover (9); Sedimentation tank (6) is arranged on one side of separation tank (1); Material conveying mechanism is arranged on separation tank (1) and sedimentation tank (6).

2. The apparatus for purifying and producing a protease according to claim 1, wherein The stirring separation mechanism includes: Driving component is arranged on separation tank (1); Separation component is arranged on driving component.

3. A device for the purification of a protease according to claim 2, characterized in that: The driving component includes driving motor (3), U-shaped plate (4), rotating shaft (11) and support disc (12), the rotating shaft (11) is rotatably connected to the lower inner wall of separation tank (1), and one end of the rotating shaft (11) is rotatably penetrated through separation tank (1) and extends to the outside of separation tank (1), the support disc (12) is fixedly connected to the circumferential surface of the rotating shaft (11), the U-shaped plate (4) is fixedly connected to the bottom of separation tank (1), the driving motor (3) is fixedly connected to the bottom of U-shaped plate (4), and the output end of the driving motor (3) is rotatably penetrated through U-shaped plate (4) and is fixedly connected with the rotating shaft (11).

4. The apparatus for purifying and producing a protease according to claim 3, wherein: The separation component includes filter cartridge A (13), filter cartridge B (15), mounting cylinder (17) and locking screw (18), the mounting cylinder (17) is movably sleeved on the rotating shaft (11), the locking screw (18) is movably inserted on the mounting cylinder (17), and one end of the locking screw (18) is threadedly connected to the rotating shaft (11), the filter cartridge A (13) is fixedly connected to the circumferential surface of the mounting cylinder (17), and the filter cartridge A (13) is rotatably arranged in separation tank (1), the filter cartridge B (15) is fixedly connected to the circumferential surface of the mounting cylinder (17), and the filter cartridge B (15) is located on the inside of the filter cartridge A (13).

5. A device for the purification and production of a protease according to claim 4, characterized in that: The scraping mechanism includes scraper A (14) and scraper B (16), the scraper A (14) is fixedly connected to the bottom of sealing cover (9), and the scraper A (14) is attached to the inner wall of filter cartridge A (13), the scraper B (16) is fixedly connected to the bottom of sealing cover (9), and the scraper B (16) is attached to filter cartridge B (15).

6. A device for the purification and production of a protease according to claim 5, characterized in that: The material conveying mechanism includes material conveying pump (5), liquid inlet pipe (7) and liquid outlet pipe (8), the material conveying pump (5) is arranged between separation tank (1) and sedimentation tank (6), the liquid inlet pipe (7) is fixedly connected between the material conveying pump (5) and the sedimentation tank (6), and the liquid outlet pipe (8) is fixedly connected between the material conveying pump (5) and the sealing cover (9).

7. A device for the purification and production of a protease according to claim 6, characterized in that: The sealing cover (9) is detachably connected to the top of separation tank (1) by bolts.

8. A device for the purification and production of a protease according to claim 7, characterized in that: The bottom of the separation tank (1) is fixedly connected with three supporting legs (2).

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