Multicolor fluorescence imaging microsphere analysis system

Abstract

The utility model belongs to the technical field of detection, and particularly relates to a multicolor fluorescence imaging microsphere analysis system which comprises a camera, a light source group, a microwell plate and a plurality of groups of microspheres, the microspheres are coupled with fluorescent dyes with three colors, the plurality of groups of microspheres are divided into at least two types, each group of microspheres is coupled with a detection target, and the color proportions of the fluorescent dyes coupled to the outer sides of the at least two types of microspheres are different; a plurality of vertically-through positioning micropores are formed in the microporous plate, and a plurality of groups of microspheres can fall into the positioning micropores; the camera is used for shooting the microspheres on the microwell plate; the light source group is used for exciting the fluorescent dye on the microspheres to irradiate three kinds of light, the light with three wavelengths is matched with the fluorescent dye with three colors respectively, and the number of the positioning micropores is 1536-100000. According to the utility model, the proportion of the fluorescent dyes with different colors coupled on different microspheres can be simply and accurately detected.

Description

Technical Field

[0001] This invention belongs to the field of detection technology, specifically relating to a multicolor fluorescence imaging microsphere analysis system. Background Technology

[0002] In numerous fields such as biomedical detection and chemical analysis, the accurate detection and analysis of different substances has always been a key research focus and a challenge. With the continuous deepening of scientific research and the increasing diversification of practical applications, the requirements for detection technologies are becoming increasingly stringent.

[0003] Traditional detection methods often have many limitations, such as low detection accuracy, complex operation, and inability to accurately detect multiple target substances at the same time, which makes it impossible for traditional detection methods to easily and accurately detect multiple detection targets. Utility Model Content

[0004] The purpose of this invention is to provide a multicolor fluorescence imaging microsphere analysis system that can relatively easily and accurately analyze the proportion of fluorescent dyes on different microspheres, thereby accurately identifying a certain microsphere.

[0005] The specific technical solution adopted by this utility model is as follows:

[0006] A multicolor fluorescence imaging microsphere analysis system includes a camera, a light source group, a microplate, and several groups of microspheres;

[0007] The microspheres are coupled with three colors of fluorescent dyes, and the microspheres in several groups are divided into at least two types. Each type of microsphere is provided with a detection target, and the proportions of the fluorescent dyes coupled to the outside of at least two types of microspheres are different.

[0008] The microporous plate has multiple vertically penetrating positioning microholes, and several sets of microspheres can fall into the multiple positioning microholes.

[0009] The camera is used to photograph the microspheres on the microporous plate;

[0010] The light source group is used to emit three types of light to irradiate the fluorescent dye on the microspheres, and the three wavelengths of light are respectively matched with the three colors of fluorescent dye.

[0011] Furthermore, the number of positioning micropores is 1536-100000.

[0012] Furthermore, the inner diameter of the upper end of the positioning micro-hole is larger than the outer diameter of the microsphere, and the inner diameter of the lower end of the positioning micro-hole is smaller than the inner diameter of the microsphere.

[0013] Furthermore, the microplate is located on the upper side of the camera.

[0014] Furthermore, the light source group includes three lamps, each of which emits light at three different wavelengths.

[0015] Furthermore, a second bracket is fixedly connected to the microporous plate, and the light source group also includes a lower support. A first bracket is fixedly connected to the lower side of the lower support, and a slide is fixedly connected to the lower support. All three lamps are fixedly connected to the slide.

[0016] The technical effects achieved by this utility model are as follows:

[0017] This invention relates to a multicolor fluorescence imaging microsphere analysis system. By using a camera to photograph the fluorescent dyes on microspheres in a microporous plate, the proportion of the three colors of fluorescent dyes on each group of microspheres can be determined. This allows for a relatively simple and accurate analysis of the proportion of fluorescent dyes on different microspheres, thereby accurately identifying a specific microsphere. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the structure of the microporous plate of this utility model;

[0020] Figure 3 This is a schematic diagram of the structure of the light source assembly of this utility model.

[0021] The attached diagram lists the components represented by each number as follows:

[0022] 100. Camera; 200. Light source assembly; 201. Support 1; 202. Lower support; 203. Carriage; 204. Lamp; 300. Microplate; 301. Positioning microholes; 302. Support 2; 400. Microsphere. Detailed Implementation

[0023] To make the objectives and advantages of this utility model clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the following text is merely used to describe one or more specific embodiments of this utility model and does not strictly limit the scope of protection specifically claimed by this utility model.

[0024] like Figure 1-3 As shown, a multicolor fluorescence imaging microsphere analysis system includes a camera 100, a light source group 200, a microplate 300, and several groups of microspheres 400.

[0025] Among them, such as Figure 1As shown, the microporous plate 300 has multiple vertically penetrating positioning microholes 301, the number of which is 1536-100000. The multiple positioning microholes 301 can be arranged in a rectangular array. When several groups of microspheres 400 fall naturally on the upper side of the microporous plate 300, they can randomly fall into the interior of multiple positioning microholes 301, so that the microspheres 400 are locked inside the interior of the positioning microholes 301. Each positioning microhole 301 can only accommodate one group of microspheres 400.

[0026] In order to make it easier for the microsphere 400 to fall into the interior of the positioning microhole 301, the shape of the positioning microhole 301 can be further improved. Specifically, the inner diameter of the upper end of the positioning microhole 301 is larger than the outer diameter of the microsphere 400, and the inner diameter of the lower end of the positioning microhole 301 is smaller than the inner diameter of the microsphere 400.

[0027] like Figure 1 As shown, the camera 100 is used to photograph the microspheres 400 on the microporous plate 300, and the microporous plate 300 is preferably located on the upper side of the camera 100. At this time, the camera 100 takes pictures from the lower side of the microporous plate 300, so that it can only take pictures of the lower side of the microspheres 400 that fall into the positioning microhole 301, so as to achieve the purpose of only photographing the microspheres 400 that fall into the positioning microhole 301.

[0028] There are several ways to mount the microperforated plate 300 on the upper side of the camera 100, such as suspending or fixing with a bracket. In this technical solution, bracket fixing is selected. Specifically, a bracket 302 is fixedly connected to the microperforated plate 300, and the microperforated plate 300 can be fixed by the bracket 302.

[0029] The microspheres 400 are coupled with three colors of fluorescent dyes. Several groups of microspheres 400 are divided into at least two types, and the proportions of the fluorescent dyes coupled to the outside of the at least two types of microspheres 400 are different. Each group of microspheres 400 is provided with a detection target, and the detection targets of multiple groups of microspheres 400 are different.

[0030] Fluorescent dyes are composed of fluorescent molecules, meaning that each type of microsphere 400 corresponds to a detection target. By detecting the proportion of fluorescent dye on the outside of microsphere 400, a certain type of microsphere 400 can be accurately identified, and thus the type of detection target can be determined.

[0031] By photographing the fluorescent dye on the microspheres 400 on the microplate 300 with camera 100, the proportion of the three colors of fluorescent dye on each group of microspheres 400 can be determined, thereby identifying the type of detection target.

[0032] In this context, the detection target can be DNA.

[0033] Among them, the light source group 200 is used to irradiate three kinds of light to irradiate the fluorescent dyes on the microspheres 400. The three kinds of light have different wavelengths, and the three wavelengths of light are respectively matched with the three fluorescent dyes. The three colors of light excite the three fluorescent dyes to produce fluorescence.

[0034] Specifically, the light source group 200 includes three lamps 204, which emit light of three different wavelengths. At this time, the microsphere 400 can be irradiated by activating the three lamps 204 in sequence, and the camera 100 can be activated to take pictures.

[0035] like Figure 1 and Figure 3 As shown, there are multiple ways to assemble the lamps 204, such as hanging or bracket fixing. In this technical solution, bracket fixing is selected. Specifically, the light source group 200 also includes a lower support 202. A bracket 201 is fixedly connected to the lower side of the lower support 202 to support the lower support 202. A slide 203 is fixedly connected to the lower support 202. All three lamps 204 are fixedly connected to the slide 203 to provide support for the lamps 204.

[0036] The working principle of this utility model is as follows:

[0037] When several sets of microspheres 400 fall naturally on the upper side of the microporous plate 300, they can fall into the interior of multiple positioning microholes 301, thereby making the microspheres 400 snap into the interior of the positioning microholes 301.

[0038] The microsphere 400 can be illuminated by activating three lamps 204 in sequence, and the camera 100 can be activated to take pictures respectively.

[0039] By photographing the fluorescent dye on the microspheres 400 on the microplate 300 with camera 100, the proportion of the three colors of fluorescent dye on each group of microspheres 400 can be determined, thus accurately identifying a certain type of microsphere 400 and determining the type of detection target.

[0040] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model, unless otherwise specified or limited, shall be implemented using conventional methods in the field.

Claims

1. A multi-color fluorescent imaging microsphere resolving system, characterized in that: The application relates to a camera (100), a light source group (200), a micro-hole plate (300) and a plurality of groups of microspheres (400). The microspheres (400) are coupled with three colors of fluorescent dyes, the plurality of groups of microspheres (400) are divided into at least two groups, each group of microspheres (400) is provided with one detection target, and the color proportion of the fluorescent dyes coupled outside the microspheres (400) in the at least two groups is different. The micro-hole plate (300) is provided with a plurality of positioning micro-holes (301) penetrating through the micro-hole plate (300) from top to bottom, and the plurality of groups of microspheres (400) can fall into the plurality of positioning micro-holes (301). The number of the positioning micro-holes (301) is 1536-100000, the inner diameter of the upper end of the positioning micro-holes (301) is larger than the outer diameter of the microspheres (400), and the inner diameter of the lower end of the positioning micro-holes (301) is smaller than the inner diameter of the microspheres (400). The camera (100) is used for photographing the microspheres (400) on the micro-hole plate (300). The light source group (200) is used for radiating three kinds of light for irradiating the fluorescent dyes on the microspheres (400), and the three kinds of light with different wavelengths are matched with the three colors of fluorescent dyes respectively. 2.The multi-color fluorescent imaging microsphere analysis system of claim 1, wherein: The micro-hole plate (300) is located on the upper side of the camera (100). 3.The multi-color fluorescent imaging microsphere analysis system of claim 1, wherein: The light source group (200) comprises three lamps (204), and the three lamps (204) radiate three kinds of light with different wavelengths respectively. 4.The multi-color fluorescent imaging microsphere analysis system of claim 3, wherein: The micro-hole plate (300) is fixedly connected with a bracket two (302), the light source group (200) further comprises a lower support (202), the lower side of the lower support (202) is fixedly connected with a bracket one (201), the lower support (202) is fixedly connected with a sliding frame (203), and the three lamps (204) are all fixedly connected on the sliding frame (203).

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