Exosome separation and extraction device

Abstract

The utility model relates to the technical field of separation and extraction devices, and discloses an exosome separation and extraction device which comprises a centrifugal machine body, a centrifugal plate, a centrifugal tube, a centrifugal pump, a centrifugal pump and a control system. A moving assembly is arranged in the centrifugal machine body. The moving assembly comprises an electric push rod fixedly connected to the interior of the centrifugal machine body. When the centrifugal machine is used, the electric push rod is started to push the moving plate to move upwards, so that the centrifugal tube can be pushed out upwards from the inside of the centrifugal disc, the extending part of the upper end of the centrifugal tube is lengthened, the centrifugal tube is convenient to take, and meanwhile, when the moving plate moves upwards, the moving seat is pushed to drive the test tube rack to move out of the inside of the centrifugal machine body; and the limiting assembly is used for limiting, so that an operator can conveniently place a test tube on which supernate is to be placed in the test tube rack without walking back to an experiment table to extract the supernate and then returning to the centrifugal machine body for centrifuging, and the operation is convenient.

Description

TECHNICAL FIELD

[0001] The utility model relates to separating and extracting device technical field, concretely is a kind of exosome separating and extracting device. BACKGROUND

[0002] Exosome refers to small membrane bubble including complex RNA and protein, and various cells can secrete exosome under normal and pathological conditions.It is mainly derived from the multivesicular body formed by endocytic lysosome microparticle, and is released into extracellular matrix after the fusion of multivesicular body outer membrane and cell membrane, and the most commonly used method for extracting exosome is ultracentrifugation method, which needs to use centrifugal equipment to purify exosome.

[0003] After the working of the separating device ends, the centrifuge tube is generally removed from the separating device, and in the existing centrifugal device, the upper end of the centrifuge tube is less exposed and is attached to the separating device, so that it is difficult to hold the centrifuge tube after centrifugation, which is inconvenient and has low applicability, and to some extent, it affects processing.In related technology, a lifting disc is arranged in the centrifugal device, the centrifuge tube is pushed upward from the inside of the separating device by lifting the lifting disc upward, the top of the centrifuge tube is moved upward, so that the centrifuge tube is conveniently taken, but the lifting disc has a single function, and when using ultracentrifugation method for centrifugation, it needs to be centrifuged three times, and the supernatant needs to be taken each time, the desktop test tube rack is far away and has no fixing device, and once the test tube is accidentally touched during extraction, the centrifuged material may be spilled, which affects the extraction efficiency. UTILITY MODEL CONTENT

[0004] (I) Technical problem solved

[0005] In view of the deficiencies of the prior art, the utility model provides an exosome separating and extracting device, which has the advantages of convenient centrifuge tube taking and providing test tube placing structure after centrifugation, and solves the problems of difficult centrifuge tube taking and tedious operation of taking supernatant by operating personnel in the existing exosome separating and extracting device.

[0006] (II) Technical solution

[0007] To achieve the above object, the utility model provides the following technical scheme: an exosome separation and extraction device, include: centrifuge body, its inside is provided with centrifugal disc, and the inside of centrifugal disc is placed with centrifugal tube, the inside of centrifuge body is provided with moving component, moving component includes: electric push rod, fixedly connected with the inside of centrifuge body, moving plate, sliding connection is established in the inside of centrifuge body, and the bottom of moving plate is fixedly connected with the top output end of electric push rod, connecting block, fixedly connected with the four corners of moving plate, first guide rod, fixedly connected with the inside of centrifuge body, and the connecting block is slidingly connected with the first guide rod, moving seat, sliding connection is established in one side of centrifuge body, test tube rack, fixedly connected with the inside of moving seat, spring return mechanism, set up in both sides of moving seat.

[0008] In some embodiments, the spring return mechanism comprises: a sliding block fixedly connected to both ends of the moving seat; a second guide rod fixedly connected to the inside of the centrifuge body, the second guide rod being slidingly connected with the sliding block, and a spring being sleeved outside the second guide rod.

[0009] In some embodiments, a limiting component is arranged on the top of the centrifuge body; the limiting component comprises: a limiting block slidingly connected to the inside of the centrifuge body; side sliding blocks fixedly connected to both sides of the limiting block; a third guide rod fixedly connected to the inside of the centrifuge body, the third guide rod being slidingly connected with the side sliding blocks, and a spring being sleeved outside the third guide rod; and a limiting groove arranged on the top of the moving seat.

[0010] In some embodiments, the bottom of the limiting block protrudes out of the inside of the centrifuge body, the protruding part is provided with an inclined surface, and the shape and size of the limiting groove are the same as those of the bottom protruding part of the limiting block.

[0011] In some embodiments, the top of the limiting block extends above the centrifuge body, and a through groove is arranged on the top of the limiting block.

[0012] In some embodiments, the moving seat, the test tube rack and the limiting component form a group, two groups of the moving seat, the test tube rack and the limiting component are symmetrically arranged on both sides of the centrifuge body.

[0013] In some embodiments, an inclined surface is arranged on the side of the moving seat close to the moving plate, and the bottom of the inclined surface is in contact with the side of the moving plate.

[0014] In some embodiments, the top of the first guide rod is at the same height as the bottom of the centrifugal disc.

[0015] (Three) beneficial effects

[0016] Compared with the prior art, the exosome separation and extraction device has the following advantages

[0017] Beneficial effects:

[0018] When the utility model is used, only need to start electric push rod, push mobile board to move upwards, can push centrifugal tube from the inside of centrifugal disc upwards, make centrifugal tube upper end stretch out part lengthen, convenient for the taking of centrifugal tube, simultaneously, when mobile board moves upwards, will push mobile seat to drive test tube rack to move from the inside of centrifugal machine body and limit through the limiting component, convenient for operating personnel to place test tube to be placed supernatant in test tube rack inside, do not have to walk back to experiment desktop to extract supernatant, return to centrifugal machine body place to carry out centrifugation, convenient operation. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is the structural schematic diagram of the utility model;

[0020] Figure 2 It is the internal structure schematic diagram of centrifugal machine body of the utility model;

[0021] Figure 3 It is the structural schematic diagram of mobile assembly of the utility model;

[0022] Figure 4 It is the structural schematic diagram of limiting component of the utility model.

[0023] In the drawing:

[0024] 11, centrifugal machine body;12, centrifugal disc;

[0025] 2, mobile assembly;21, electric push rod;22, mobile board;23, connecting block;24, No. 1 guide rod;25, mobile seat;26, test tube rack;27, spring return mechanism;271, sliding block;272, No. 2 guide rod;

[0026] 3, limiting component;31, limiting block;32, side sliding block;33, No. 3 guide rod;34, limiting groove. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0028] It should be noted that all directionality indications in the embodiments of the present application are only used to explain the relative positional relationship, movement condition and the like between components in a certain posture, and if the certain posture changes, the directionality indications also change accordingly.

[0029] In the present application, unless specifically defined and limited otherwise, the terms "connection", "fixation" and the like should be understood in a broad sense, for example, "fixation" can be fixed connection, or detachable connection, or integral; can be mechanical connection, or electrical connection; can be direct connection, or indirect connection through intermediate medium; can be internal connection of two elements or interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0030] In addition, the description involving "first", "second" and the like in the present application is only for the purpose of description, and cannot be understood as indicating or implying the relative importance of the indicated technical features or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features. In addition, the technical solutions of various embodiments can be combined with each other, but it must be based on the fact that a person skilled in the art can realize it, when the combination of technical solutions contradicts each other or cannot be realized, it should be considered that the combination of technical solutions does not exist, and is not within the protection scope required by the present application.

[0031] In use, after centrifugation, the operator takes out the centrifugal tube, moves the supernatant of the centrifugal tube to the tabletop, and then takes the supernatant to the centrifuge for centrifugation, which is repeated three times, and then ultracentrifugation is performed.

[0032] In the related art, a lifting disc is arranged in the centrifugal device, the centrifugal tube is pushed upward from the inside of the separation device by lifting the lifting disc upward, the top of the centrifugal tube is moved upward, thereby facilitating the taking of the centrifugal tube, but the function of the lifting disc is relatively single, and when using the ultracentrifugation method for centrifugation, three times of centrifugation are required, and the supernatant needs to be taken each time. The tabletop test tube rack is far away and has no fixing device, and once an accidental touch occurs during extraction, the centrifuged material may be spilled, causing the extraction efficiency to be affected.

[0033] In order to solve the problems in the related art to some extent, the embodiment of the present application provides an exosome separation and extraction device. When in use, only the electric push rod 21 needs to be started to push the moving plate 22 to move upwards, the centrifugal tube can be pushed out from the inside of the centrifugal disc 12 upwards, the part of the centrifugal tube extending out of the centrifugal disc 12 is lengthened, the centrifugal tube is convenient to take, and at the same time, when the moving plate 22 moves upwards, the moving seat 25 is pushed to move the test tube rack 26 out of the inside of the centrifuge body 11 and is limited by the limiting assembly 3, the operator can conveniently place the test tube for placing supernatant in the inside of the test tube rack 26, it is not necessary to go back to the experiment desktop to extract the supernatant and then go back to the centrifuge body 11 to centrifuge, and the operation is convenient.

[0034] The present application will be described below in combination with the drawings and with reference to specific embodiments:

[0035] In combination Figures 1-4 , the embodiment of the present application provides an exosome separation and extraction device, which comprises: a centrifuge body 11, the inside of the centrifuge body 11 is provided with a centrifugal disc 12, and the inside of the centrifugal disc 12 is provided with a centrifugal tube; the inside of the centrifuge body 11 is provided with a moving assembly 2; the moving assembly 2 comprises: an electric push rod 21, which is fixedly connected to the inside of the centrifuge body 11; a moving plate 22, which is slidably connected to the inside of the centrifuge body 11, and the bottom of the moving plate 22 is fixedly connected with the top output end of the electric push rod 21; a connecting block 23, which is fixedly connected to the four corners of the moving plate 22; a first guide rod 24, which is fixedly connected to the inside of the centrifuge body 11, and the connecting block 23 is slidably connected with the first guide rod 24; a moving seat 25, which is slidably connected to one side of the centrifuge body 11; a test tube rack 26, which is fixedly connected to the inside of the moving seat 25; and a spring return mechanism 27, which is arranged on both sides of the moving seat 25.

[0036] When in use, after the centrifugation of the test tube in the inside of the centrifugal disc 12 is completed, the electric push rod 21 is started to push the moving plate 22 to move upwards, in the process of moving upwards of the moving plate 22, the connecting block 23 slides along the first guide rod 24, the first guide rod 24 limits the sliding of the connecting block 23, the connecting block 23 is offset, and then the moving plate 22 is prevented from being offset when moving, with the moving plate 22 moving upwards, the bottom of the centrifugal tube in the inside of the centrifugal disc 12 is pushed, the centrifugal tube is pushed upwards, the part of the centrifugal tube extending out of the centrifugal disc 12 is lengthened, the centrifugal tube is convenient to take, with the moving upwards of the moving plate 22, the side slope of the moving seat 25 is gradually pressed, and then the moving seat 25 is pushed to move towards the outside of the centrifuge body 11, until the test tube rack 26 is completely pushed out of the inside of the centrifuge body 11, the test tube rack 26 can conveniently place the test tube beside the centrifuge body 11, and then the supernatant in the centrifugal tube is convenient to move and take, the stability of the test tube rack 26 is ensured by the moving seat 25, and the risk of the test tube rack 26 moving after being touched is reduced.

[0037] In some embodiments, the spring return mechanism 27 comprises: sliding blocks 271 fixedly connected to both ends of the moving seat 25; a second guide rod 272 fixedly connected to the inside of the centrifuge body 11, the second guide rod 272 being in sliding connection with the sliding blocks 271, and the outside of the second guide rod 272 being sleeved with a spring; when the moving plate 22 moves upward, the moving seat 25 moves away from the inside of the centrifuge body 11, at this time, the sliding blocks 271 will compress the second guide rod 272; when centrifuging, the moving plate 22 moves downward, gradually releasing the pressure on one side of the moving seat 25, and the moving seat 25 is pushed by the sliding blocks 271 to the inside of the centrifuge body 11 under the action of the springs outside the second guide rod 272 on both sides, so as to put the test tube rack 26 into the centrifuge body 11 during centrifuging, preventing the test tube rack 26 from being collided by the operator.

[0038] In some embodiments, the top of the centrifuge body 11 is provided with a limiting assembly 3; the limiting assembly 3 comprises: a limiting block 31 in sliding connection with the inside of the centrifuge body 11; side sliding blocks 32 fixedly connected to both sides of the limiting block 31; a third guide rod 33 fixedly connected to the inside of the centrifuge body 11, the third guide rod 33 being in sliding connection with the side sliding blocks 32, and the outside of the third guide rod 33 being sleeved with a spring; and a limiting groove 34 formed in the top of the moving seat 25.

[0039] In use, when the moving seat 25 moves toward the outside of the centrifuge body 11, the moving seat 25 will contact one side of the limiting block 31, the limiting block 31 is retracted into the inside of the centrifuge body 11 by pressing one side of the limiting block 31, and then the side sliding blocks 32 compress the springs outside the third guide rod 33; when the limiting groove 34 reaches directly below the limiting block 31, the moving seat 25 no longer presses the limiting block 31, and the limiting block 31 is pushed into the limiting groove 34 by the side sliding blocks 32 under the action of the springs outside the third guide rod 33, thereby limiting the moving seat 25 and ensuring the stability of the moving seat 25.

[0040] In some embodiments, the bottom of the limiting block 31 protrudes out of the inside of the centrifuge body 11, the protruding part is provided with an inclined surface, and the shape and size of the limiting groove 34 are the same as those of the protruding part of the bottom of the limiting block 31, thereby enhancing the stability of the limiting of the moving seat 25.

[0041] In some embodiments, the top of the limiting block 31 extends above the centrifuge body 11, and the top of the limiting block 31 is provided with a through slot, facilitating pulling the limiting block 31 to pull the limiting block 31 out of the inside of the limiting groove 34, and facilitating unlocking the moving seat 25.

[0042] In some embodiments, the mobile seat 25, the test tube rack 26 and the limiting assembly 3 are a group, two groups are arranged on both sides of the centrifuge body 11, and are symmetrically arranged, the number of test tube racks 26 is increased, and the operator can further classify the used and unused test tubes, and the use is convenient.

[0043] In some embodiments, the mobile seat 25 is provided with an inclined surface on one side close to the mobile plate 22, and the bottom of the inclined surface is in contact with one side of the mobile plate 22, the inclined surface can be pressed by the movement of the mobile plate 22, and the mobile seat 25 is driven to move.

[0044] In some embodiments, the top of the first guide rod 24 is at the same height as the bottom of the centrifugal disc 12, and the movement of the mobile plate 22 is limited, so that the mobile plate 22 is prevented from pushing the centrifugal disc 12 when moving upwards.

[0045] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "an example", "a specific example" or "some examples" means that the specific features, structures, materials or characteristics described in combination with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in the present specification.

[0046] In addition, the technical solutions of each embodiment can be combined with each other, but it must be based on the realization of ordinary skilled in the art, when the combination of technical solutions appears contradictory or cannot be realized, it should be considered that the combination of technical solutions does not exist, also not within the scope of protection required by the present application.

[0047] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations of the embodiments can be made without departing from the principles and spirits of the present application, the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. An exosome separation and extraction device, characterized in that, include: A centrifuge body (11) has a centrifuge tray (12) inside, and centrifuge tubes are placed inside the centrifuge tray (12); a moving component (2) is provided inside the centrifuge body (11); the moving component (2) includes: An electric push rod (21) is fixedly connected to the inside of the centrifuge body (11); a movable plate (22) is slidably connected to the inside of the centrifuge body (11), and the bottom of the movable plate (22) is fixedly connected to the top output end of the electric push rod (21); a connecting block (23) is fixedly connected to the four corners of the movable plate (22); a first guide rod (24) is fixedly connected to the inside of the centrifuge body (11), and the connecting block (23) is slidably connected to the first guide rod (24); a movable seat (25) is slidably connected to one side of the centrifuge body (11); a test tube rack (26) is fixedly connected to the inside of the movable seat (25); and a spring reset mechanism (27) is provided on both sides of the movable seat (25).

2. The exosome separation and extraction device according to claim 1, characterized in that, The spring reset mechanism (27) includes: A sliding block (271) is fixedly connected to both ends of the movable seat (25); a second guide rod (272) is fixedly connected to the inside of the centrifuge body (11), the second guide rod (272) is slidably connected to the sliding block (271), and a spring is sleeved on the outside of the second guide rod (272).

3. The exosome separation and extraction device according to claim 1, characterized in that, A limiting component (3) is provided on the top of the centrifuge body (11); the limiting component (3) includes: A limiting block (31) is slidably connected to the inside of the centrifuge body (11); a side slider (32) is fixedly connected to both sides of the limiting block (31); a third guide rod (33) is fixedly connected to the inside of the centrifuge body (11), the third guide rod (33) is slidably connected to the side slider (32), and a spring is sleeved on the outside of the third guide rod (33); a limiting groove (34) is opened on the top of the movable seat (25).

4. The exosome separation and extraction device according to claim 3, characterized in that: The bottom of the limiting block (31) extends into the interior of the centrifuge body (11), the extended part is provided with an inclined surface, and the shape and size of the limiting groove (34) are the same as the bottom extended part of the limiting block (31).

5. The exosome separation and extraction device according to claim 3, characterized in that: The top of the limiting block (31) extends above the centrifuge body (11), and the top of the limiting block (31) has a through groove.

6. The exosome separation and extraction device according to claim 3, characterized in that: The movable seat (25), the test tube rack (26) and the limiting component (3) are a set, and two sets are provided on both sides of the centrifuge body (11), and they are arranged symmetrically.

7. The exosome separation and extraction device according to claim 1, characterized in that: The movable seat (25) has an inclined surface on the side near the movable plate (22), and the bottom of the inclined surface is in contact with one side of the movable plate (22).

8. The exosome separation and extraction device according to claim 1, characterized in that: The top of the first guide rod (24) is at the same height as the bottom of the centrifugal disc (12).

Images