Excrement sample transfer device for PCR (Polymerase Chain Reaction) experiment

Abstract

The utility model discloses an excrement sample transfer device for a PCR (Polymerase Chain Reaction) experiment, which comprises a main shell and an excrement box input unit, and the excrement box input unit comprises a sliding base body and a placing cavity; a sample tube input unit; the excrement sample transferring unit is arranged in the internal working area and is used for transferring an excrement part in an excrement box in the excrement box input unit to a sample tube in the sample tube input unit; the device comprises a translation driving assembly, a steering motor fixedly arranged at the output end of the translation driving assembly, a telescopic cylinder fixedly connected with a rotating shaft of the steering motor, and a chuck assembly arranged at the front end of the telescopic cylinder. And the swab rod assembly is mounted in the swab pre-mounting area. Clinical practical application working conditions are considered, common excrement boxes and test tubes are used as input, the operation of transferring excrement in the excrement boxes into the test tubes is achieved through an automatic means, on one hand, manual operation is omitted, the rejection feeling of medical workers on the operation process is avoided, on the other hand, manual operation is replaced with a mechanical mode, and the labor intensity is reduced. And the sample transfer processing efficiency is greatly improved.

Description

TECHNICAL FIELD

[0001] The embodiment of the utility model belongs to the technical field of sample transfer, more particularly, relate to a kind of excrement sample transfer device for PCR experiment. BACKGROUND

[0002] Excrement inspection refers to analyzing excrement by physical, immunological, chemical, biological and other methods, understanding the function of digestive system, and assisting in diagnosing digestive tract infection, hemorrhage, malignant tumor and other diseases. Excrement inspection generally includes PCR nucleic acid examination, microscopic examination, occult blood test and fecal bile determination. Existing bacterial examination is generally microscopic examination, but if in the early stage of pathological change, the pathogenic bacteria or nucleic acid characteristic quantity in excrement sample is less, long time sample processing consumption, easy to cause sample quality change in process, greatly influence the examination result, and further delay the best treatment opportunity. And in clinical operation, sample pretreatment process is complicated, and a large amount of manual operation is required, and the labor intensity of experimental personnel is large, and the experimental result may fluctuate due to the operation difference of experimental personnel, not only lengthen sample processing time, and affect the stability of test result. Therefore, it is of great significance to improve the working efficiency and the accuracy of detection result to propose a kind of excrement sample transfer device for PCR experiment.

[0003] Chinese invention patent CN114323850B discloses a sample pretreatment device and a nucleic acid and fecal sample pretreatment method. The sample pretreatment device includes a machine body assembly, which includes a rack, a TIP rack, a sample tube rack, an uncapping module, a mechanical arm module, a nucleic acid extraction module and a control module. The uncapping module is arranged on the rack and is used to open and close the tube cover of the test tube. The mechanical arm module is arranged on the rack and is used to transfer the test tube and transfer the solution through the TIP gun head. The nucleic acid extraction module is arranged on the rack and is used to extract the nucleic acid product of the sample. According to the sample pretreatment device of the embodiment of the present application, the actions of the mechanical arm module, the uncapping module and the nucleic acid extraction module can be controlled by the control module to perform sample pretreatment, thereby replacing manual operation during sample pretreatment, realizing full-process automation of sample pretreatment, and thereby facilitating reduction of labor intensity of experiment personnel and improvement of stability and consistency of experimental results. In addition, Chinese invention patent CN113188865B discloses a pretreatment device for fecal sample detection in an intensive care unit, which includes a shell and a sample processing cavity. The fecal sample is placed in a sealed disposable sampling cup and is fixed in the sample processing cavity for pretreatment. A bacterial culture mechanism and a liftable sample processing probe are arranged in the shell. The sample processing probe includes a column with a tapered tip at the bottom and has a treatment liquid pipeline in communication with a treatment liquid input pipe. After the treatment liquid pipeline pierces the fecal sample in the disposable sampling cup at the bottom end of the sample processing probe, the treatment liquid is injected into the fecal sample. The present application can process the fecal sample, so that the trace bacteria in the fecal sample are proliferated before inspection, which are more easily detected. Not only is the detection time of the fecal sample greatly shortened and the detection efficiency is improved, but more importantly, the trace pathogenic bacteria contained therein can be more accurately detected, the disease can be detected in advance, and the treatment opportunity is avoided.

[0004] The above-mentioned patent technologies replace manual operation by using automatic devices, and to a certain extent, improve the fecal sample pretreatment efficiency. However, there are still the following technical problems: (1) The patent technology CN113188865B uses a disposable fecal sample placement container. Under normal circumstances, a toilet needs to be reserved for one week for review. The disposable toilet structure cannot meet the storage requirement after being pierced, and therefore the piercing sampling method used in the technology has certain defects; (2) The patent technology CN114323850B integrates the subsequent centrifugation, oscillation and extraction operations of the sampling tube containing the fecal sample, but does not consider the process of transferring the fecal sample from the toilet to the sampling tube. This process not only brings discomfort to medical personnel, but also greatly reduces the overall operation efficiency. Practical new type content

[0005] In view of the above defects or improvement needs of the prior art, the utility model provides a kind of excrement sample transfer device for PCR experiment.Considering clinical practical application working condition, commonly used chamber pot and test tube are used as input, and the transfer operation of excrement in chamber pot to test tube is realized by automatic means, which on the one hand saves manual operation, avoids the repulsion of medical staff to operation process, on the other hand, replaces manual operation with mechanization, greatly improves sample transfer processing efficiency.

[0006] In order to achieve the above purpose, a kind of excrement sample transfer device for PCR experiment, comprising:

[0007] Main shell, including chamber pot inlet and sample tube inlet arranged on the left and right sides of the main shell, internal working area located in the interior of the main shell, swab preloading area arranged on the top of the main shell, swab storage area arranged at the bottom of the main shell;

[0008] Chamber pot input unit horizontally slidingly installed at the chamber pot inlet, comprising sliding base body horizontally sliding with the chamber pot inlet, and placing cavity opened on the upper surface of the sliding base body;Sample tube input unit horizontally slidingly installed at the sample tube inlet;

[0009] Excrement sample transfer unit arranged in the internal working area for transferring excrement in chamber pot in chamber pot input unit to sample tube in sample tube input unit, comprising translation drive assembly, steering motor fixedly arranged on the output end of the translation drive assembly, telescopic cylinder fixedly connected with the rotating shaft of the steering motor, and chuck assembly arranged at the front end of the telescopic cylinder;Swab rod assembly installed in the swab preloading area.

[0010] Preferably, the chamber pot input unit comprises a clamping assembly for clamping a container in the placing cavity.

[0011] Preferably, the clamping assembly comprises:

[0012] Sliding cavity opened on the side wall of the sliding base body, sliding piston horizontally slidingly installed in the sliding cavity, push spring arranged between the sliding piston and the right side of the sliding cavity, and clamping push plate fixedly connected with the left side of the sliding piston through extension rod, and the clamping push plate is located in the placing cavity.

[0013] Preferably, the sample tube input unit and the chamber pot input unit have the same structure.

[0014] Preferably, the translation drive assembly comprises:

[0015] A horizontal translation drive motor fixed to the side of the internal work area, a translation drive screw rod fixed horizontally and coaxially to the output shaft of the translation drive motor, and a translation drive nut linearly driven by the translation drive screw rod; the steering motor is fixedly connected to the translation drive nut.

[0016] Preferably, the chuck assembly comprises:

[0017] A clamping drive motor fixed to the front end of the telescopic cylinder, a reverse screw rod fixed horizontally and coaxially to the clamping drive motor, a pair of drive nut blocks horizontally connected to the reverse screw rod, and a chuck arranged below the drive nut blocks.

[0018] Preferably, the swab storage area side wall is provided with an air outlet.

[0019] Overall, compared with the prior art, the above technical scheme conceived by the present application can achieve the following beneficial effects:

[0020] (1) The PCR experiment fecal sample transfer device of the present application considers the actual application conditions in clinical practice, uses a common toilet and a test tube as input, and realizes the transfer operation of the feces in the toilet to the test tube through automatic means. On the one hand, the device saves manual operation and avoids the rejection of medical staff to the operation process. On the other hand, the device replaces manual operation with mechanization, greatly improving the sample transfer processing efficiency.

[0021] (2) The PCR experiment fecal sample transfer device of the present application additionally provides a clamping assembly in the toilet input unit and the sample tube input unit, and fixes the toilet or the sampling tube relative to the sliding base body by means of the elastic component, thereby preventing the toilet or the sampling tube from shaking during the sliding process of the sliding base body and causing the internal sample to splash out. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 It is a whole structure schematic view of the PCR experiment fecal sample transfer device of the present application;

[0023] Figure 2 It is a chuck assembly structure schematic view of the PCR experiment fecal sample transfer device of the present application;

[0024] Figure 3 It is a toilet input unit structure schematic view of the PCR experiment fecal sample transfer device of the present application;

[0025] In all the drawings, the same reference signs refer to the same technical features, specifically: 1 - main housing, 101 - cassette inlet, 102 - sample tube inlet, 103 - internal working area, 104 - swab pre-loading area, 105 - swab disposal area, 2 - cassette input unit, 201 - sliding base, 202 - placement cavity, 210 - clamping assembly, 211 - sliding cavity, 212 - push spring, 213 - sliding piston, 214 - clamping push plate, 3 - fecal sample transfer unit, 310 - translation drive assembly, 311 - translation drive motor, 312 - translation transmission screw, 313 - translation transmission nut, 320 - telescopic cylinder, 330 - chuck assembly, 331 - clamping drive motor, 332 - reverse screw, 333 - transmission nut block, 334 - chuck, 340 - steering motor, 4 - air outlet, 5 - swab rod assembly, 6 - sample tube input unit. DETAILED DESCRIPTION

[0026] In the description of the present application, it needs to be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0027] In addition, the terms "first", "second" are only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise specifically limited.

[0028] In the present application, unless otherwise specifically defined and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the communication between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0029] In order to make the purpose, technical scheme and advantages of the utility model clearer and more apparent, the utility model will be described in further detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the utility model and not to limit the utility model. In addition, the technical features involved in each embodiment of the utility model described below can be combined with each other as long as they do not conflict with each other.

[0030] As Figures 1-3 shown in the utility model embodiment, the kind of PCR experiment uses excrement sample transfer device, including:

[0031] The main shell 1 includes the toilet entrance 101 and the sample tube entrance 102 arranged on the left and right sides of the main shell 1, the internal working area 103 located in the main shell 1, the swab preloading area 104 arranged on the top of the main shell 1, the swab discard area 105 arranged at the bottom of the main shell 1;

[0032] The toilet input unit 2 horizontally slidingly installed at the toilet entrance 101 includes the sliding base body 201 horizontally sliding with the toilet entrance 101, and the placement cavity 202 is opened on the upper surface of the sliding base body 201, and the sample tube input unit 6 is horizontally slidingly installed at the sample tube entrance 102;

[0033] The excrement sample transfer unit 3 arranged in the internal working area 103 is used to transfer the excrement in the toilet in the toilet input unit 2 to the sample tube in the sample tube input unit 6, which includes the translation drive assembly 310, the steering motor 340 fixedly arranged on the output end of the translation drive assembly 310, the telescopic cylinder 320 fixedly connected with the rotating shaft of the steering motor 340, the chuck assembly 330 arranged at the front end of the telescopic cylinder 320, and the swab rod assembly 5 installed in the swab preloading area 104.

[0034] As Figure 1 and Figure 3 shown in the utility model embodiment, the toilet input unit 2 includes the clamping assembly 210 for clamping the container in the placement cavity 202.

[0035] As Figure 1 and Figure 3 shown in the utility model embodiment, the clamping assembly 210 includes:

[0036] The sliding cavity 211 is opened on the side wall of the sliding base body 201, the sliding piston 213 is horizontally slidingly installed in the sliding cavity 211, the thrust spring 212 is arranged between the sliding piston 213 and the right side surface of the sliding cavity 211, the clamping push plate 214 is fixedly connected to the left side surface of the sliding piston 213 through the extension rod, and the clamping push plate 214 is located in the placement cavity 202.

[0037] As Figure 1 shown in the utility model embodiment, the sample tube input unit 6 and the convenient box input unit 2 structure same composition.

[0038] As Figure 1 shown in the utility model embodiment, the translation drive assembly 310, including:

[0039] Horizontal fixed in the inside work area 103 side plane translation drive motor 311, keep horizontal direction with the translation drive motor 311 output shaft coaxial fixed connection translation transmission screw rod 312, with the translation transmission screw rod 312 keep linear transmission translation transmission nut 313, the steering motor 340 with translation transmission nut 313 fixed connection.

[0040] As Figure 1 and Figure 2 shown in the utility model embodiment, the chuck assembly 330, including:

[0041] Fixed in the telescopic cylinder 320 front end clamping drive motor 331, keep horizontal direction with the clamping drive motor 331 keep coaxial fixed connection reverse screw rod 332, a pair of transmission nut block 333 that horizontal transmission connection in the reverse screw rod 332, be equipped with chuck 334 in the transmission nut block 333 below.

[0042] As Figure 1 shown in the utility model embodiment, the swab discard storage area 105 side wall is equipped with exhaust port 4.

[0043] The utility model discloses a working principle: first, the excrement box is placed in the placing cavity 202 in the convenient box input unit 2, and the excrement sample sampling tube is placed in the placing cavity 202 of sample tube input unit 6, and then the convenient box input unit 2 and sample tube input unit 6 are pushed into the internal working area 103, then, start the system, control the rotation of the translation drive motor 311, thereby drive the horizontal movement of the translation transmission nut 313, control the rotation of the steering motor 340 simultaneously, and make the chuck assembly 330 towards the swab rod assembly 5 in the upper direction, then control the extension of the telescopic cylinder 320, and control the chuck assembly 330 clamping action cooperation, complete the clamping operation of a swab, then control the rotation of the steering motor 340, make the chuck assembly 330 towards the lower direction, and control the chuck assembly 330 to move to the excrement box directly above, thereby control the extension of the telescopic cylinder 320, make the front end of the swab contact and adhere with the excrement, then retract the telescopic cylinder 320, then, control the chuck assembly 330 to move to the test tube directly above, and control the extension of the telescopic cylinder 320 again, insert the swab into the test tube preloaded with physiological saline, complete the excrement sample transfer, then, make the chuck assembly 330 move to the entrance of the swab storage area 105 directly above, and control the chuck assembly 330 to release the clamping of the swab, make the swab fall into the swab storage area 105, complete a sample transfer.

[0044] In the utility model embodiment, considering the clinical actual application working condition, commonly used convenient box and test tube are taken as input, and the transfer operation of excrement in the convenient box to the test tube is realized through automatic means, the device avoids the repulsion of medical staff to the operation process on the one hand, and replaces manual operation in a mechanized mode, greatly improves the sample transfer processing efficiency.

[0045] In addition, in the utility model, the chucking assembly 210 is additionally arranged in the convenient box input unit 2 and the sample tube input unit 3, the convenient box or the sampling tube is relatively fixed with the sliding base body through the elastic component, so that the technical problem that the convenient box or the sampling tube shakes and causes the internal sample to splash during the sliding process of the sliding base body is solved.

[0046] The above is only the preferred embodiment of the present application, and does not limit the present application, and any modification, equivalent replacement and improvement made within the spirit and principle of the present application should be included in the protection scope of the present application. The above is only the preferred embodiment of the present application, and it should be pointed out that, for ordinary skilled in the art, on the premise of not departing from the technical principles of the present application, a plurality of improvements and modifications can be made, and these improvements and modifications should be regarded as the protection scope of the present application.

Claims

1. A fecal sample transfer device for PCR experiments, characterized by, The utility model relates to a fecal sample collection device, including: A main shell (1) including a toilet bowl inlet (101) and a sample tube inlet (102) arranged on the left and right sides of the main shell (1), an internal working area (103) located inside the main shell (1), a swab preloading area (104) arranged on the top of the main shell (1), and a swab storage area (105) arranged at the bottom of the main shell (1); A toilet bowl input unit (2) horizontally slidingly installed at the toilet bowl inlet (101), including a sliding base body (201) horizontally sliding with the toilet bowl inlet (101), and a placement cavity (202) opened on the upper surface of the sliding base body (201); and a sample tube input unit (6) horizontally slidingly installed at the sample tube inlet (102); A fecal sample transfer unit (3) arranged in the internal working area (103) for transferring the fecal matter in the toilet bowl in the toilet bowl input unit (2) to the sample tube in the sample tube input unit (6), including a translation driving assembly (310), a steering motor (340) fixedly arranged at the output end of the translation driving assembly (310), a telescopic cylinder (320) fixedly connected with the rotating shaft of the steering motor (340), and a chuck assembly (330) arranged at the front end of the telescopic cylinder (320); and a swab rod assembly (5) installed in the swab preloading area (104).

2. The fecal sample transfer device for PCR experiments according to claim 1, characterized in that, The toilet bowl input unit (2) includes a clamping assembly (210) for clamping the container in the placement cavity (202).

3. The device of claim 2, wherein, The clamping assembly (210) includes: a sliding cavity (211) opened on the side wall of the sliding base body (201), a sliding piston (213) horizontally slidingly installed in the sliding cavity (211), a push spring (212) arranged between the sliding piston (213) and the right side of the sliding cavity (211), and a clamping push plate (214) fixedly connected with the left side of the sliding piston (213) through an extension rod, the clamping push plate (214) being located in the placement cavity (202).

4. The fecal sample transfer device for PCR experiments according to claim 3, characterized in that, The sample tube input unit (6) has the same structure as the toilet bowl input unit (2).

5. The device of claim 1, wherein, The translation driving assembly (310) includes: a translation driving motor (311) horizontally fixed to the side of the internal working area (103), a translation transmission screw rod (312) fixedly connected with the output shaft of the translation driving motor (311) in a coaxial manner in the horizontal direction, and a translation transmission nut (313) linearly driven with the translation transmission screw rod (312); the steering motor (340) is fixedly connected with the translation transmission nut (313).

6. The fecal sample transfer device for PCR experiments of claim 1, wherein, The chuck assembly (330) includes: a clamping driving motor (331) fixedly arranged at the front end of the telescopic cylinder (320), a reverse screw rod (332) fixedly connected with the clamping driving motor (331) in a coaxial manner in the horizontal direction, a pair of transmission nut blocks (333) horizontally transmissionally connected with the reverse screw rod (332), and a chuck (334) arranged below the transmission nut blocks (333).

7. The device according to any one of claims 1 to 6, wherein An air outlet (4) is arranged on the side wall of the swab storage area (105).

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