Suspension cell culture bag

Abstract

The utility model discloses a suspension cell culture bag which comprises a supporting base, a limiting box is installed at one end of the supporting base, a cell bag is arranged in the limiting box, a material injection opening is formed in the upper portion of the cell bag, and a driving motor is installed at one end of the limiting box. The discharging pipe is installed at the bottom of the cell bag, a control valve is installed on the discharging pipe, a sampling bin is connected to the lower portion of the discharging pipe, a sampling opening is formed in the lower portion of the sampling bin, and a sealing plug is installed below the sampling opening. Compared with an existing device, the suspension cell culture bag has the advantages that the cell bag can be supported and limited through the limiting box, and the driving motor can drive the limiting box to rotate, so that the cell bag can be shaken, cells in the cell bag can be uniformly mixed with a culture solution, and cell sap in the sampling bin can be sampled through the sampling port; therefore, the condition of the cells can be known in time.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture technical field, concretely is a kind of suspension cell culture bag. BACKGROUND

[0002] Suspension cell is the cell that cell growth does not depend on support surface, cell growth does not depend on support surface, and it is in suspension state growth in culture solution, and it needs to use suspension cell culture bag in suspension cell culture process.

[0003] The Chinese patent with the authorized publication number CN220116568U discloses a kind of suspension cell culture bag, including bag body, injection pipe is equipped on bag body, injection pipe end is injection port, the bag body is divided into multiple independent partitions by at least one adhesion zone, and each partition is equipped with a sampling port.The suspension cell culture bag provided by the utility model can minimize the occurrence of situations such as no cell growth in new bag, insufficient cell purity, etc., so that subsequent cell experiments can be carried out normally.

[0004] Although the suspension cell culture bag in the above can minimize the occurrence of situations such as no cell growth in new bag, insufficient cell purity, etc., but suspension cell culture cannot be supported and fixed, and during cell culture process, the device is relatively shareholder, it is not convenient to make the cells in suspension cell culture bag mix evenly, which affects the development of cells, and sampling observation is not convenient during culture process, therefore, we propose a kind of suspension cell culture bag. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a kind of suspension cell culture bag, to solve the problem that suspension cell culture cannot be supported and fixed in the above background art, and during cell culture process, the device is relatively shareholder, it is not convenient to make the cells in suspension cell culture bag mix evenly, which affects the development of cells, and sampling observation is not convenient during culture process.

[0006] To achieve the above object, the utility model provides the following technical scheme: a kind of suspension cell culture bag, comprising:

[0007] supporting seat, one end of the supporting seat is equipped with limit box, and cell bag is placed in the inside of limit box, injection port is arranged above the cell bag, and drive motor is installed in one end of limit box;

[0008] downcomer, which is installed at the bottom of the cell bag, the control valve is installed on the downcomer, the sampling bin is connected below the downcomer, and the sampling port is arranged below the sampling bin, and the sealing plug is installed below the sampling port.

[0009] Preferably, the injection port and the cell bag are in communication with each other, and the size of the cell bag matches the size of the limit box.

[0010] Preferably, the limiting box and the cell bag form a semi-enclosed structure, and the cell bag and the limiting box are detachably connected.

[0011] Preferably, the cell bag is further provided with:

[0012] An injection pipe opening is mounted on one side above the cell bag, and a sealing cover is mounted on the injection pipe opening.

[0013] Preferably, the injection pipe opening and the cell bag are in communication with each other, the sealing cover and the injection pipe opening are detachably connected, and the driving motor and the limiting box form a rotating structure.

[0014] Preferably, the cell bag forms a communication structure between the blanking pipe and the sampling bin, and the control valve is fixedly connected with the blanking pipe.

[0015] Preferably, the sampling bin and the sampling port are in communication with each other, and the sealing plug and the sampling port are detachably connected.

[0016] Compared with the prior art, the utility model provides a kind of suspended cell culture bag, with the following beneficial effects:

[0017] The utility model can support and limit the cell bag by limiting box, driving motor can drive limiting box to rotate, so as to shake the cell bag, it is convenient for the cell in cell bag to mix evenly, the cell liquid in sampling bin can be sampled by sampling port, so as to be convenient for understanding the condition of cell in time, avoid that suspended cell culture cannot be supported and fixed, and in the process of cell culture, device is relative shareholder, it is not convenient to make the cell in suspended cell culture bag mix evenly, influence the development of cell, and it is inconvenient to sample and observe in the process of culture. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is overall structure schematic diagram of the utility model;

[0019] Figure 2 It is structure schematic diagram of cell bag of the utility model;

[0020] Figure 3 It is structure schematic diagram of support seat and limiting box of the utility model;

[0021] Figure 4 It is side view structure schematic diagram of the utility model.

[0022] In the drawing: 1, support seat;2, limiting box;3, cell bag;4, injection port;5, injection pipe opening;6, control valve;7, blanking pipe;8, sampling bin;9, sampling port;10, sealing plug;11, driving motor;12, sealing cover. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the utility model will be apparently and completely described in connection with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all the other embodiments obtained by the ordinary skilled in the art without creative labor belong to the scope of protection of the utility model.

[0024] Please refer to Figures 1-3 A kind of suspension cell culture bag, comprising: support seat 1, one end of support seat 1 is equipped with limiting box 2, and the inside of limiting box 2 is placed with cell bag 3, the top of cell bag 3 is provided with injection port 4, injection port 4 is communicated with cell bag 3, and the size of cell bag 3 and limiting box 2 is matched;Cell and cell culture solution can be put into cell bag 3 by injection port 4;Limiting box 2 and cell bag 3 form semi-enclosed structure, and cell bag 3 and limiting box 2 are detachably connected;Limiting box 2 can support and limit cell bag 3;One end of limiting box 2 is equipped with drive motor 11;Downpipe 7 is installed at the bottom of cell bag 3, control valve 6 is installed on downpipe 7, cell bag 3 forms communication structure between downpipe 7 and sampling bin 8, and control valve 6 is fixedly connected with downpipe 7;Through downpipe 7, cell liquid in cell bag 3 can be conveniently circulated to sampling bin 8, and control valve 6 can conveniently open and close downpipe 7;Sampling bin 8 is connected below downpipe 7, sampling port 9 is arranged below sampling bin 8, and sealing plug 10 is installed below sampling port 9;Sampling bin 8 and sampling port 9 are communicated, and sealing plug 10 and sampling port 9 are detachably connected;Through sampling port 9, cell liquid in sampling bin 8 can be sampled, so that the condition of cell can be conveniently understood in time, and sealing plug 10 can conveniently open and close sampling port 9.

[0025] Please refer to Figure 1 、 3 And 4, a kind of suspension cell culture bag, comprising: injection pipe port 5, it is installed on the side above cell bag 3, sealing cover 12 is installed on injection pipe port 5;Injection pipe port 5 can conveniently inject nutrient solution etc. in cell bag 3 during cell culture process, and sealing cover 12 can guarantee the sealing property of injection pipe port 5;Injection pipe port 5 and cell bag 3 are communicated, and sealing cover 12 and injection pipe port 5 are detachably connected, and drive motor 11 and limiting box 2 form rotating structure;Drive motor 11 can drive limiting box 2 to rotate, so as to shake cell bag 3, and facilitate the mixture of cell and culture solution in cell bag 3.

[0026] Working principle: when using the suspension cell culture bag, firstly, the cells and cell culture solution are put into the cell bag 3 through the feeding opening 4, and then the cell bag 3 is placed into the limiting box 2, so that the cell bag 3 is supported and limited; secondly, the nutrient solution and the like are injected into the cell bag 3 through the injection pipe opening 5 during the cell culture process, and the sealing cover 12 can ensure the sealing of the injection pipe opening 5; then the driving motor 11 can drive the limiting box 2 to rotate, so that the cell bag 3 can be shaken, and the cells in the cell bag 3 can be mixed with the culture solution uniformly; finally, when sampling is needed, the control valve 6 is opened, the cell solution in the cell bag 3 is conveniently flowed into the sampling bin 8 through the discharging pipe 7, and the cell solution in the sampling bin 8 can be sampled through the sampling opening 9, so that the condition of the cells can be understood in time, and the sealing plug 10 can conveniently open and close the sampling opening 9, which is the working principle of the suspension cell culture bag.

[0027] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A suspension cell culture bag, characterized in that, Include: Support seat (1), one end of support seat (1) is installed with limit box (2), and cell bag (3) is arranged in limit box (2), injection port (4) is arranged above cell bag (3), and drive motor (11) is installed at one end of limit box (2); The blanking tube (7) is installed at the bottom of the cell bag (3), the control valve (6) is installed on the blanking tube (7), the sampling bin (8) is connected below the blanking tube (7), the sampling port (9) is arranged below the sampling bin (8), and the sealing plug (10) is installed below the sampling port (9).

2. The suspension cell culture bag of claim 1, wherein The injection port (4) and the cell bag (3) are communicated with each other, and the size of the cell bag (3) and the limit box (2) is matched.

3. The suspension cell culture bag of claim 1, wherein The limit box (2) and the cell bag (3) constitute a semi-enclosed structure, and the cell bag (3) and the limit box (2) are detachably connected.

4. The suspension cell culture bag of claim 1, wherein, The cell bag (3) is also provided with: Injection tube port (5) is installed on one side above the cell bag (3), sealing cover (12) is installed on the injection tube port (5).

5. The suspension cell culture bag of claim 4, wherein, The injection tube port (5) and the cell bag (3) are communicated with each other, the sealing cover (12) and the injection tube port (5) are detachably connected, and the drive motor (11) and the limit box (2) constitute a rotating structure.

6. The suspension cell culture bag of claim 1, wherein, The cell bag (3) constitutes a communication structure between the blanking tube (7) and the sampling bin (8), and the control valve (6) and the blanking tube (7) are fixedly connected.

7. The suspension cell culture bag of claim 1, wherein The sampling bin (8) and the sampling port (9) are communicated with each other, and the sealing plug (10) and the sampling port (9) are detachably connected.

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