Drug sensitivity testing device

Abstract

The utility model relates to a drug sensitivity test device, which is characterized in that before the test is started, a growth identification light source is utilized to irradiate a culture component, and a light source detection module receives growth information obtained from the number of original specimens after irradiation so as to judge whether the culture is finished or not; the method comprises the following steps: diluting a sample, adding the diluted sample into a reaction tank of a reagent disc, integrally placing the diluted sample into a detection setting tank to start a test, sequentially irradiating the same reaction tank with a first detection light source and a second detection light source with different wavelengths after a reaction, and finally converting color information obtained from an AST special indicator into wavelength information by a spectrum processing module to obtain a detection result. Therefore, the growth condition and the detection result of the specimen can be accurately judged in a simple and rapid manner, and the detection result is analyzed by the two detection light sources and the spectrum processing module, so that manual interpretation is avoided, and a more accurate result can be obtained through cross comparison.

Description

TECHNICAL FIELD

[0001] The utility model provides a kind of drug sensitivity test device of simple and fast mode accurate judgment drug sensitivity test growth condition of specimen before and after test and detection result. BACKGROUND

[0002] Antimicrobial Susceptibility Testing (AST), or antimicrobial susceptibility testing, is a test designed to assess the susceptibility of bacteria, fungi or other microorganisms to antimicrobial drugs (such as antibiotics) in order to select the most effective treatment drug.

[0003] AST experiments require detection of specimen concentration or specimen volume before and after the experiment. The pre-experiment detection is aimed at the microbial culture condition, and the microorganisms in the culture dish must reach a certain concentration to provide the required amount of specimen for subsequent experiments. Post-experiment detection is aimed at the reaction condition of microorganisms and antibiotics, and the number of microorganisms after reaction must be confirmed to determine whether the antibiotic meets the experimental requirements.

[0004] The above-mentioned specimen detection of AST experiment has the following problems and deficiencies that need to be improved:

[0005] First, when detecting the number of microorganisms in the culture dish, the microorganisms are usually grown for several or tens of hours under specific temperature conditions, and the culture time is used as a judgment of whether the microorganisms have reached a certain concentration. However, considering the growth conditions and conditions of different microorganisms, using such a general culture method to judge whether the microorganisms are growing normally, even with a reminder function, still requires manual inspection of the results. If there is human interpretation of the growth conditions, the culture dish will be repeatedly removed and reinserted into the space suitable for growth. This will result in judgment errors, unknown specimen concentration or specimen volume, and the risk of infection from repeated removal and inspection.

[0006] Second, the traditional AST experiment result determination is by Kirby-Bauer disc diffusion method, and the sensitivity of the specimen to the antibiotic is determined by measuring the size of the inhibition zone. This method has a high risk of misjudgment. Although there is a way to determine the color depth after the color indicator reacts, it still belongs to manual inspection, and the accuracy and correctness of the experimental results need to be discussed.

[0007] How to solve the above-mentioned problems and deficiencies of the conventional method is the direction of research and improvement that the applicant and relevant manufacturers in this industry are eager to improve. SUMMARY

[0008] The utility model discloses a main purpose at: utilize the design of growth discernment light source and light source detection module, can accurately judge the growth condition of the specimen in the culture assembly, avoid artificial judgment and reduce the infection risk, and can know the concentration of specimen growth simultaneously.

[0009] The utility model discloses a main purpose at: utilize the design of first detection light source, second detection light source and spectrum processing module, with the cross comparison of different wavelength illumination, the color information of the special indicator of drug sensitivity test is converted into wavelength information, and the detection result is obtained quickly and accurately.

[0010] To achieve the above purpose, the structure of the utility model includes: a test device, at least two detection setting slots, a reagent disc, a plurality of reaction tanks for containing an AST special indicator, at least two culture assemblies for containing an original specimen, a growth discernment light source, at least two first detection light sources, at least two second detection light sources with different wavelengths from the first detection light sources, at least one light source detection module, and at least one spectrum processing module. The detection setting slots are arranged in the test device, the reaction tanks are arranged in the reagent disc, each culture assembly is arranged on one side of each detection setting slot, the growth discernment light source is arranged on one side of the culture assembly and irradiates the original specimen, the first detection light source is arranged on one side of the detection setting slot and irradiates any reaction tank, the second detection light source is arranged on one side of the detection setting slot and irradiates the same reaction tank as the first detection light source, the light source detection module receives the growth information obtained from the number of original specimens after the culture assembly is irradiated by the growth discernment light source, and the spectrum processing module receives the color information obtained from the AST special indicator after the reaction tank is irradiated by the first detection light source and the second detection light source, and converts the color information into wavelength information.

[0011] Before the user uses the utility model for drug sensitivity test, the original specimen is first placed in the culture assembly in the test device for pre-culture. During the culture period, the growth discernment light source can be used to irradiate the culture assembly, and the light source detection module receives the growth information obtained from the number of original specimens after the culture assembly is irradiated by the growth discernment light source, to determine whether the culture is a usable specimen. Then, the usable specimen is diluted into a suitable specimen, and is added to the reaction tank of the reagent disc, which is then placed in the detection setting slot of the test device to start the test device. After the reaction is completed, the first detection light source and the second detection light source with different wavelengths are used to irradiate the suitable specimen in the same reaction tank, and finally the spectrum processing module receives the color information of the AST special indicator in the reaction tank after being irradiated, and converts the color information into wavelength information to obtain the detection result. In this way, the growth condition and the detection result of the specimen are accurately determined in a simple and fast manner, and the detection result is analyzed by two detection light sources and the spectrum processing module, which not only avoids manual interpretation, but also obtains more accurate results through cross comparison.

[0012] By the above-mentioned technology, the artificial judgment error, unknown sample concentration or sample volume, easy infection by multiple sample taking and viewing, and low accuracy and correctness of experimental result by artificial viewing can be solved. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 The expanded perspective view of the preferred embodiment of the present application.

[0014] Figure 2 The exploded view of the preferred embodiment of the present application.

[0015] Figure 3 The cross-sectional view of the preferred embodiment of the present application.

[0016] Figure 4 The detection schematic diagram of the culture assembly of the preferred embodiment of the present application.

[0017] Figure 5 The sample adding schematic diagram of the preferred embodiment of the present application.

[0018] Figure 6 The reaction tank detection schematic diagram of the preferred embodiment of the present application.

[0019] Figure 7 The sample recognition schematic diagram of the preferred embodiment of the present application.

[0020] Figure 8 The display schematic diagram of the preferred embodiment of the present application.

[0021] Figure 9 The uniform temperature schematic diagram of the preferred embodiment of the present application.

[0022] Figure 10 The spotlight schematic diagram of the preferred embodiment of the present application.

[0023] SYMBOL DESCRIPTION

[0024] Test device... 1

[0025] Detection setting groove... 11

[0026] Partition member... 12

[0027] Cover body... 13

[0028] Recognition reading part... 14

[0029] Display screen... 15

[0030] Networking module... 16

[0031] Concentrating module...17

[0032] Reagent discs...2

[0033] Reaction tank...21

[0034] AST-specific indicator...211

[0035] Cultivation Components...3

[0036] Growth identification light source...4

[0037] Light source detection module...41

[0038] First detection light source...51

[0039] Second detection light source...52

[0040] Spectrum processing module...53

[0041] High-speed drive module...54

[0042] Electronic devices...6

[0043] Heating device...7

[0044] Temperature equalization channel...71

[0045] Temperature sensor...72

[0046] Original specimen...A1

[0047] Container Identification Unit...A11

[0048] Available Specimens...A2

[0049] Appropriate specimen...A3

Detailed Implementation Methods

[0050] To achieve the above objectives and effects, the technical means and structure adopted by this utility model are described in detail below with reference to the preferred embodiment of this utility model.

[0051] Please see Figures 1 to 6 The figure shown is an unfolded perspective view of a preferred embodiment of the present invention, showing a schematic diagram of the reaction tank inspection. The figure clearly shows that the present invention includes:

[0052] A test device 1;

[0053] At least two detection setting slots 11 are provided in the testing device 1 for respectively setting a reagent disc 2 having a plurality of reaction slots 21 for containing an antimicrobial susceptibility testing (AST) specific indicator 211;

[0054] A partition member 12 is provided between each of the detection setting slots 11, and a cover 13 is movably provided on each of the detection setting slots 11;

[0055] At least two culture assemblies 3 are respectively provided on one side of each of the detection setting slots 11 for containing an original sample A1;

[0056] A growth recognition light source 4 is provided on one side of the culture assembly 3 and irradiates the original sample A1 for confirming the growth condition thereof;

[0057] At least two first detection light sources 51 are respectively provided on one side of each of the detection setting slots 11 and irradiate any of the reaction slots 21;

[0058] At least two second detection light sources 52 having different wavelengths from the first detection light sources 51 are respectively provided on one side of each of the detection setting slots 11 and irradiate the same reaction slots 21 as the first detection light sources 51;

[0059] At least one light source detection module 41 receives growth information obtained from the quantity of the original sample A1 after the culture assembly 3 is irradiated by the growth recognition light source 4; and

[0060] At least one spectrum processing module 53 receives color information obtained from the AST specific indicator 211 after the reaction slots 21 are irradiated by the first detection light sources 51 and the second detection light sources 52, and converts the color information into wavelength information.

[0061] The test device 1 is a box structure with two lids 13 which can be opened independently. The detection setting groove 11 is a groove-shaped containing space in the test device 1, which has a setting area for setting the reagent disc 2. The culture assembly 3 is located at one side of the setting area in the test device 1. In this embodiment, the culture bottles located at the corners of the two detection setting grooves 11 are taken as examples. The growth recognition light source 4 is a light-emitting assembly with a specified wavelength. The light source detection module 41 is a light receiver corresponding to the growth recognition light source 4, and has a chip with a growth state calculation function. In this embodiment, the growth recognition light source 4 and the light source detection module 41 are arranged on the opposite side walls of the setting seat of the culture assembly 3. The first detection light source 51 and the second detection light source 52 are light-emitting assemblies with specified wavelengths. The spectrum processing module 53 is a light receiver corresponding to the first detection light source 51 and the second detection light source 52, and has a chip with a color information conversion function. In this embodiment, the first detection light source 51 and the second detection light source 52 are arranged on the lid 13 of the test device 1, and the irradiation direction is controlled to irradiate in the same reaction groove 21. In principle, the irradiation angle of the first detection light source 51 and the second detection light source 52 is fixed, and the reagent disc 2 can be rotated to irradiate in different reaction grooves 21 each time. The spectrum processing module 53 is arranged on the bottom surface of the test device 1 adjacent to the reaction groove 21. Alternatively, the chips of the light source detection module 41 and the spectrum processing module 53 can be integrated into a processing chip, which is arranged on the test device 1 and electrically connected thereto, to distinguish the light receiving function and the calculation function. The corresponding types of the above components are only examples of the preferred embodiments, and any type with the same function is within the scope of the present application, and is not limited to the above examples.

[0062] According to the above description, the structure of the present technology can be understood, and according to the corresponding cooperation of the structure, the growth condition and the detection result of the specimen before and after the AST test can be accurately judged in a simple and fast manner. As shown in the figure, before the user uses the drug sensitivity test of the present application, the original specimen A1 is first placed in the culture assembly 3 in the test device 1 for pre-culture. During the culture period, the growth recognition light source 4 can be used to irradiate the culture assembly 3, and the growth information obtained from the number change of the original specimen A1 is received by the light source detection module 41 to determine whether the original specimen A1 has been cultured into a usable specimen A2. The light source detection module 41 in this embodiment uses the turbidimetry method for detection. The turbidimetry method is a method for evaluating the concentration of suspended particles in a solution. Therefore, the light emitted by the growth recognition light source 4 passes through the culture assembly 3, and the light intensity passing through the bacterial solution is measured by the light source detection module 41. The decrease of the light intensity is proportional to the concentration of the specimen in the bacterial solution. The McFarland standard turbidity can be used to standardize the concentration of the bacterial solution to ensure the consistency of the concentration of the bacterial solution in different experiments. Alternatively, a standard curve of known specimen concentration can be established to compare the detection data with the standard curve, so as to determine the concentration of the specimen in the bacterial solution. This ensures that the specimen is stable and has sufficient concentration before the test, so as to avoid invalid test.

[0063] Then, the usable specimen A2 is diluted with culture solution to form a proper specimen A3, and is added to the reaction groove 21 of the reagent disc 2. The detection setting groove 11 of the test device 1 is used to start the test device 1. In order to make the reaction conditions of each reaction groove 21 the same, an equal amount of AST special indicator 211 is arranged in the reaction groove 21, and the centrifugal force is used to evenly distribute the proper specimen A3 to each reaction groove 21. The diluted proper specimen A3 is more conducive to the above distribution action. The dilution of the usable specimen A2 can also make the baseline of the bacterial solution concentration consistent and more accurately control the concentration of the proper specimen A3 before the reaction.

[0064] After the reaction is completed, the testing device 1 automatically irradiates the proper test object A3 in the same reaction tank 21 with the first detection light source 51 and the second detection light source 52 of different wavelengths in sequence. Since the proper test object A3 produces color changes after reacting with the AST special indicator 211, the color changes are different according to different AST special indicators 211. For example, when the AST special indicator 211 uses resazurin indicator, it produces three color changes of blue, purple and pink. When the AST special indicator 211 uses 2,3,5-chlorinated triphenyl tetrazolium indicator, it produces color changes of colorless, light pink and deep pink. This embodiment uses the AST special indicator 211 using resazurin indicator as an example. Resazurin indicator is a kind of redox indicator, which is blue in color. Therefore, the color of the test object before growth is blue. Once the NADH dehydrogenase in the test object mitochondria is reduced to purple or pink, NAD is nicotinamide (Nicotinamide Adenine Dinucleotide, NAD), dehydrogenase coenzyme, and NADH is the reduced state of nicotinamide. Therefore, the color before the reaction is detected by the first detection light source 51 with an absorbance value OD 600-580 nm, and the color after the reaction is detected by the second detection light source 52 with an absorbance value OD 580-560 nm. When the AST special indicator 211 uses 2,3,5-chlorinated triphenyl tetrazolium indicator, it is detected by an absorbance value OD 245-248 nm.

[0065] After the first detection light source 51 and the second detection light source 52 irradiate the AST special indicator 211, the color information is transmitted to the spectral processing module 53 from the reaction tank 21. The spectral processing module 53 receives the irradiated color information and converts it into wavelength information to obtain the detection result. The detection result is no longer a general color, but a specific number. Therefore, by comparing the wavelength information before and after the reaction, abnormal data can be easily excluded, or the data result with the most obvious judgment standard can be selected. Since the color change of the AST special indicator 211 is gradual, the color information converted into wavelength information is actually a spectral interval. As shown in Table 1, the best detection wavelengths of the present application are 600 nm of the first detection light source 51 and 570 nm of the second detection light source 52. For example, the wavelength information of four groups of bacterial liquid before and after the reaction is (0.928,

[0066] 0.198), (0.666, 0.43), (0.535, 0.566) and (0.377, 0.523), thus, the color change determined by human judgment can have errors, and the closer the data before and after the reaction, the more obvious the antibacterial effect of the antibiotic. Therefore, the growth status and detection results of the sample can be accurately determined in a simple and fast manner, and the detection results are analyzed by two detection light sources and the spectrum processing module 53, which not only avoids manual interpretation, but also can obtain more accurate results through cross comparison.

[0067] Table 1

[0068] Pre-reaction colour Blue Blue Blue Blue Post-reaction colour Red Purple Deep purple Blue OD570 value 0.928 0.666 0.535 0.37 OD600 value 0.198 0.43 0.566 0.523 Colour determination Red Purple Purple Blue Spectral region 480-600 480-640 500-640 500-640

[0069] In addition, the test device 1 has two detection setting grooves 11, a partition member 12 is arranged between the two detection setting grooves 11, and each has a cover 13, so that the two detection setting grooves 11 can be independently operated, the partition member 12 can isolate the two detection setting grooves 11 to avoid mutual infection of the samples, and the cover 13 can not only have the functions of dustproof and anti-pollution, but also remind the user of the working state of the test device 1, such as a safety mechanism that can be started only when the cover 13 is closed. Therefore, the operation of the test device 1 is more convenient and efficient.

[0070] The test device 1 has a high-speed driving module 54, which rotates the reagent disc 2 to drive the bubbles generated in the reaction groove 21 away from the center position before the first detection light source 51 and the second detection light source 52 are actuated. The high-speed driving module 54 is a chip installed with control software (such as arranged on one side of the spectrum processing module 53), or can be integrated into the chip of the spectrum processing module 53, which can be implemented in cooperation with the timer and the rotating motor in the test device 1.

[0071] After the sample is mixed with the AST special indicator 211, it needs to be left for a period of time for reaction, and bubbles can be generated in the biochemical reaction process, which can cause the light of the first detection light source 51 and the second detection light source 52 to deviate, thereby affecting the reading signal action of the spectrum processing module 53. Therefore, after the standing time ends and before the first detection light source 51 and the second detection light source 52 are actuated, the high-speed driving module 54 in the test device 1 is used to rotate the reagent disc 2 at a high speed for a short time, so as to use the centrifugal force to drive the bubbles in the reaction groove 21 away from the center position, thereby ensuring that the spectrum processing module 53 can correctly and stably receive the light source signal.

[0072] Please refer to Figure 8 and Figure 9As shown, is the body recognition and display schematic diagram of another preferred embodiment of the utility model, from the figure can be clearly seen, this embodiment and the above-mentioned embodiment are similar, only the test device 1 outside is equipped with an identification reading part 14, the identification reading part 14 is the combination of lens and identification module in this embodiment, by this, before starting the test device 1, the container identification part A11 of the original sample A1 is read by the identification reading part 14 outside the test device 1, wherein the identification module can carry out the identification of the container identification part A11 such as text, bar code or QR code, so that the user can confirm whether the use disc item is correct when carrying out the sample culture action, which can avoid the misjudgment problem of the naked eye identification of the user, improve the overall operation convenience and efficiency, and because the identification action does not contact the test device 1, the identification action can also avoid affecting the operation stability of the test device 1, of course, the judgment basis can be established in the database first The data of the corresponding material, will not be elaborated.

[0073] In addition, the test device 1 is provided with a display screen 15, which is electrically connected to the spectrum processing module 53, and a networking module 16 is provided in the test device 1, and the detection result of the spectrum processing module 53 is transmitted to an electronic device 6, and the chip of the light source detection module 41 and the spectrum processing module 53 is integrated into a processing chip in this embodiment, which is arranged on the test device 1 and is electrically connected thereto. In this way, the color information, wavelength information or detection result obtained by the spectrum processing module 53 can be directly displayed on the display screen 15, so that the user does not need to connect an additional transmission line or connect to other display equipment, and the experimental results can be more directly known, and the color information, wavelength information or detection result obtained by the spectrum processing module 53 can be transmitted to a remote electronic device 6 such as a mobile phone by using the networking module 16, and the content on the display screen 15 can be displayed on the screen of the electronic device 6.

[0074] Please refer to Figure 9 and Figure 10As shown, it is the temperature uniformity diagram and the light condensing diagram of another preferable embodiment of the utility model, from the figure can be clear, this embodiment and the above-mentioned embodiment are big same, only on the testing device 1 with a heating device 7, and a heating device 7 side and communicate each detection setting groove 11's temperature uniformity channel 71, and according to each detection setting groove 11 in a temperature detector 72, to maintain the temperature in each detection setting groove 11. Therefore, two temperature detectors 72 will detect the temperature in detection setting groove 11 respectively, and according to the temperature set by heating device 7 heating, only when some factors (such as one side in the open cover operation) cause temperature to reduce, in order to avoid the influence of the reduced temperature to the temperature in another detection setting groove 11, as long as any temperature detector 72 feedback temperature is lower than the set value, testing device 1 will automatically start heating device 7, blow out the hot air of the set temperature value, and through the temperature uniformity channel 71 of the communication of two detection setting grooves 11, hot air is sent to two detection setting grooves 11, in this way, the lower temperature can be warmed by hot air, and the normal temperature is not affected because the hot air temperature is equivalent to it, and the purpose of maintaining the temperature uniformity in two detection setting grooves 11 is achieved.

[0075] In addition to the first detection light source 51 and the second detection light source 52 side in the same detection setting groove 11, a light condensing assembly 17 is arranged, so that the light is concentrated in the reaction tank 21, the light condensing assembly 17 in the embodiment is a kind of cover body of truncated cone, the inner side wall has the effect of reflecting light, only the front end can be transparent, the front end can be hollowed out or be provided with convex lens to condense light, in this way, convergent beam can be achieved, so that detection light source converges and accurately projects in reagent disc reaction tank 21, avoids scattering influence, so that spectral processing module 53 more accurately collects data.

[0076] The above-mentioned is only the preferable embodiment of the utility model, not therefore limit the patent range of the utility model, so for the simple modification and equivalent structural change of the utility model specification and drawing content, should be contained in the patent range of the utility model.

Claims

1. A device for drug sensitivity testing, characterized in that, The test device comprises: a test device; at least two detection setting slots arranged in the test device for arranging a reagent disc respectively, and the reagent disc has a plurality of reaction slots for containing an indicator for drug sensitivity test; at least two culture assemblies arranged on one side of each detection setting slot for containing an original sample; a growth identification light source arranged on one side of each culture assembly and irradiating the original sample for confirming its growth condition; at least two first detection light sources arranged on one side of each detection setting slot and irradiating any reaction slot; at least two second detection light sources with different wavelengths from the first detection light sources arranged on one side of each detection setting slot and irradiating the same reaction slot as the first detection light sources; at least one light source detection module for receiving the growth information obtained from the quantity of the original sample after the culture assembly is irradiated by the growth identification light source; and at least one spectrum processing module for receiving the color information obtained from the indicator after the reaction slot is irradiated by the first detection light source and the second detection light source, and converting the color information into wavelength information.

2. The apparatus for drug sensitivity testing of claim 1, wherein, The test device is provided with an identification reading part outside, and the test device is provided with a display screen electrically connected to the spectrum processing module, and the test device is provided with a networking module for transmitting the detection results of the spectrum processing module to an electronic device.

3. The apparatus for drug sensitivity testing of claim 1, wherein, The test device is provided with a heating device and a uniform temperature channel arranged on one side of the heating device and connected to each detection setting slot, and the temperature in each detection setting slot is maintained according to a temperature detector in each detection setting slot.

4. The apparatus for drug sensitivity testing of claim 1, wherein, The first detection light source and the second detection light source on one side of the same detection setting slot are provided with a light condensing assembly to concentrate light in the reaction slot.

5. The apparatus for drug sensitivity testing of claim 1, wherein, The test device is provided with a high-speed driving module to rotate each reagent disc to drive the bubbles generated in each reaction slot away from the center position before each first detection light source and each second detection light source is actuated.

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